{"id":85989,"date":"2017-06-13T06:30:06","date_gmt":"2017-06-12T22:30:06","guid":{"rendered":"\/\/m.iemloyee.com\/?p=85989"},"modified":"2017-06-10T12:25:08","modified_gmt":"2017-06-10T04:25:08","slug":"%e6%96%af%e5%9d%a6%e7%a6%8f%e5%a4%a7%e5%ad%a6nat-nanotech-%ef%bc%9a%e6%8e%a2%e6%b5%8b%e6%b4%bb%e7%bb%86%e8%83%9e%e5%86%85%e5%90%9e%e4%bd%9c%e7%94%a8%e8%86%9c%e6%9b%b2%e7%8e%87%e7%9a%84%e7%ba%b3","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=85989","title":{"rendered":"\u65af\u5766\u798f\u5927\u5b66Nat. Nanotech.\uff1a\u63a2\u6d4b\u6d3b\u7ec6\u80de\u5185\u541e\u4f5c\u7528\u819c\u66f2\u7387\u7684\u7eb3\u7c73\u7ea7\u64cd\u4f5c"},"content":{"rendered":"

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j\uff09\u4eceDNM2-GFP\u76844\u5206\u949f\u5f71\u7247\u4e2d\u622a\u53d6\u7684\u65f6\u95f4\u5e73\u5747\u7684\u56fe\u7247\u663e\u793aDNM2-GFP\u8868\u73b0\u51fa\u5bf9\u5c16\u9510\u7684\u7eb3\u7c73\u67f1\u7684\u5f3a\u70c8\u504f\u597d\uff0c\u4f46\u5728\u66f4\u5927\u534a\u5f84\u7eb3\u7c73\u67f1\uff08\u76f8\u540c\u7ec6\u80de\u4e2d\uff09\u7684\u6761\u4ef6\u4e0b\uff0c\u8fd9\u79cd\u504f\u597d\u7a0b\u5ea6\u5c06\u4f1a\u51cf\u5f31\u3002\u6bd4\u4f8b\u5c3a\uff1a10\u03bcm\u3002<\/p>\n

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i\uff09\u7528CellMask Deep Red\u67d3\u8272\u7eb3\u7c73CUI\u9635\u5217\u4e0a\u7684\u7ec6\u80de\u7684\u56fe\u50cf\uff0c\u5176\u663e\u793a\u7ec6\u80de\u819c\u5305\u88f9\u5728\u7eb3\u7c73C\u548c\u7eb3\u7c73U\u7ed3\u6784\u7684\u5185\u5916\u8868\u9762\u3002\u6bd4\u4f8b\u5c3a\uff1a2\u03bcm\u3002<\/p>\n

j\uff09\u4ece4\u5206\u949f\u5f71\u7247\u4e2d\u622a\u53d6\u7684\u65f6\u95f4\u5e73\u5747\u7684\u8367\u5149\u56fe\u7247\u663e\u793a\uff0c\u7f51\u683c\u86cb\u767d\u548c\u53d1\u52a8\u86cb\u767d2\u66f4\u504f\u597d\u7eb3\u7c73C\u548c\u7eb3\u7c73U\u7684\u672b\u7aef\u548c\u6b63\u819c\u66f2\u7387\u7684\u5916\u8868\u9762\u3002\u6bd4\u4f8b\u5c3a\uff0c2\u03bcm\u3002<\/p>\n

k\uff0951\u5bf9\u7eb3\u7c73C\u548c\u7eb3\u7c73U\u4e0a\u7684CLTA-RFP\u548cDNM2-GFP\u7684\u5e73\u5747\u56fe\u50cf\u6e05\u695a\u5730\u663e\u793a\u4e86\u5176\u7aef\u90e8\u548c\u5916\u8868\u9762\u4e0a\u7684\u79ef\u805a\u66f4\u591a\u3002\u6bd4\u4f8b\u5c3a\uff1a2\u03bcm\u3002<\/p>\n

l\uff09\u4ee5\u80de\u8d28\u4f5c\u4e3a\u53c2\u8003\uff0c\u5728\u7eb3\u7c73C\u548c\u7eb3\u7c73U\u7ed3\u6784\u7684\u672b\u7aef\uff0c\u5916\u8868\u9762\u548c\u5185\u8868\u9762\u7684\u91cf\u5316\u8367\u5149\u5f3a\u5ea6\u3002\u7edf\u8ba1\u5b66\u5206\u6790\u663e\u793a\u5728\u672b\u7aef\u3001\u5916\u8868\u9762\u4e0e\u5185\u8868\u9762\u7684\u5dee\u5f02\u663e\u8457\u3002P\u503c\u5982\u56fe\u6240\u793a\u3002<\/p>\n

\u56fe3 \u5229\u7528\u7eb3\u7c73\u68d2\u9635\u5217\u63a2\u6d4b\u4e0d\u540c\u79cd\u7c7b\u5185\u541e\u86cb\u767d\u7684\u66f2\u7387\u7075\u654f\u5ea6<\/strong><\/p>\n

\"\"<\/p>\n

a\uff09CME\u4e2d\u4e0d\u540c\u9636\u6bb5\u86cb\u767d\u8d28\u7684\u793a\u610f\u56fe\u3002<\/p>\n

b-o.\u9ad8\u500d\u8367\u5149\u56fe\u50cf\uff08\u7eff\u8272\uff1b\u6bd4\u4f8b\u5c3a\uff1a2\u03bcM\uff09\u663e\u793a\u7684\u662f\u57286\u4e2a\u7eb3\u7c73\u68d2\u4e2d\u4e0d\u540c\u86cb\u767d\u8d28\u7684\u5206\u914d\u60c5\u51b5\u3002\u6570\u767e\u7eb3\u7c73\u7684\u5e73\u5747\u56fe\u50cf\u548c\u6cbf\u7eb3\u7c73\u68d2\u957f\u5ea6\u7684\u5f3a\u5ea6\u5728\u8367\u5149\u56fe\u4e2d\u5982\u56fe\u6240\u793a\u3002<\/p>\n

b\uff09\u819c\u8054\u86cb\u767dmCherry CAAX\u3002<\/p>\n

c\uff09\u67d3\u8272\u5242\u5728\u7eb3\u7c73\u68d2\u7684\u6574\u4e2a\u957f\u5ea6\u4e0a\u76f8\u5bf9\u5747\u5300\u5730\u5206\u5e03\u3002\u516b\u79cd\u6709\u4f9d\u8d56\u6027\u5185\u541e\u4f5c\u7528\u7684\u7f51\u683c\u86cb\u767d\u6210\u5206\u4e2d\u542bepsin1-GFP\u3002<\/p>\n

d\uff09\u8f6c\u94c1\u86cb\u767d\u53d7\u4f53\uff08TfnR-GFP\uff09\u7684\u9ad8\u500d\u8367\u5149\u56fe\u50cf\u3002<\/p>\n

e\uff09\u56fe\u50cf\u663e\u793a\u51fa\u5bf9\u7eb3\u7c73\u68d2\u672b\u7aef\u7684\u8f7b\u5fae\u504f\u597d\u3002\u53e6\u4e00\u65b9\u9762\uff0c\u7528PH-GFP\u63a2\u6d4bPIP2\u3002<\/p>\n

f\uff09amphiphysin1-YFP\u86cb\u767d\u7684\u9ad8\u500d\u8367\u5149\u56fe\u50cf\u3002<\/p>\n

g\uff09CLTA-RFP\u86cb\u767d\u7684\u9ad8\u500d\u8367\u5149\u56fe\u50cf\u3002<\/p>\n

h\uff09DNM2-GFP\u86cb\u767d\u7684\u9ad8\u500d\u8367\u5149\u56fe\u50cf\u3002<\/p>\n

i\uff09AP2-GFP\u86cb\u767d\u7684\u9ad8\u500d\u8367\u5149\u56fe\u50cf\u3002<\/p>\n

j\uff09intersectin-GFP\u86cb\u767d\u7684\u9ad8\u500d\u8367\u5149\u56fe\u50cf\u3002<\/p>\n

k\uff09cortactin-GFP\u86cb\u767d\u7684\u9ad8\u500d\u8367\u5149\u56fe\u50cf\u3002<\/p>\n

i\uff09\u7528\u73af\u80bd\u67d3\u8272\u8fc7\u7684\u808c\u52a8\u86cb\u767d\u9ad8\u500d\u8367\u5149\u56fe\u50cf\u3002<\/p>\n

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p\uff09\u901a\u8fc7\u7eb3\u7c73\u68d2\u672b\u7aef\u4e0e\u7eb3\u7c73\u68d2\u4e2d\u5fc3\u7684\u5f52\u4e00\u5316\u5f3a\u5ea6\u6bd4\uff0c\u5b9a\u91cf\u5f97\u5230\u7684\u7eb3\u7c73\u68d2\u4e0a14\u79cd\u4e0d\u540c\u86cb\u767d\u8d28\/\u67d3\u6599\u7684\u5206\u5e03\u60c5\u51b5\u3002\u5bf9\u4e8e\u6bcf\u4e00\u79cd\u86cb\u767d\u8d28\uff0c\u5176\u5206\u5e03\u662f\u5bf9\u5e73\u5747\u503c\u8d85\u8fc756-2,072\u7684\u7eb3\u7c73\u68d2\u6d4b\u91cf\u5f97\u5230\u7684\u3002\u6bcf\u4e2a\u86cb\u767d\u8d28\u4e0eCellMask\u7684\u7edf\u8ba1\u5b66\u610f\u4e49\uff0c\u901a\u8fc7Welch\u6821\u6b63\u7684\u975e\u914d\u5bf9t\u68c0\u9a8c\u8fdb\u884c\u8bc4\u4f30\u3002<\/p>\n

\u56fe4 \u9884\u5f2f\u66f2\u7684\u819c\u662f\u5185\u541e\u4f5c\u7528\u7684\u4f18\u9009\u4f4d\u70b9<\/strong><\/p>\n

\"\"<\/p>\n

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\u3010\u5c0f\u7ed3\u3011<\/strong><\/p>\n

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\u6587\u732e\u94fe\u63a5\uff1aNanoscale manipulation of membrane curvature for probing endocytosis in live cells<\/a><\/strong>\uff08Nat. Nanotech.\uff0c2017\uff0cDOI:10.1038\/nnano.2017.98\uff09<\/p>\n

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