{"id":81023,"date":"2017-05-20T06:30:28","date_gmt":"2017-05-19T22:30:28","guid":{"rendered":"\/\/m.iemloyee.com\/?p=81023"},"modified":"2017-05-16T21:54:32","modified_gmt":"2017-05-16T13:54:32","slug":"%e5%9b%bd%e5%ae%b6%e7%ba%b3%e7%b1%b3%e7%a7%91%e5%ad%a6%e4%b8%ad%e5%bf%83%e9%99%88%e6%98%a5%e8%8b%b1acs-nano%ef%bc%9a%e6%89%8b%e6%80%a7%e8%a1%a8%e9%9d%a2%e5%86%b3%e5%ae%9a%e8%bd%ac%e9%93%81%e8%9b%8b","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=81023","title":{"rendered":"\u56fd\u5bb6\u7eb3\u7c73\u79d1\u5b66\u4e2d\u5fc3\u9648\u6625\u82f1ACS Nano\uff1a\u624b\u6027\u8868\u9762\u51b3\u5b9a\u8f6c\u94c1\u86cb\u767d\u53d6\u5411\u53ca\u5176\u4e0e\u53d7\u4f53\u7684\u76f8\u4e92\u4f5c\u7528"},"content":{"rendered":"

\u3010\u5f15\u8a00\u3011<\/strong><\/p>\n

\u7eb3\u7c73\u9897\u7c92\u66b4\u9732\u751f\u7406\u73af\u5883\u65f6\uff0c\u5f62\u6210\u201c\u86cb\u767d\u8d28\u7535\u6655\u201d\uff0c\u5176\u6781\u5927\u5730\u51b3\u5b9a\u4e86\u5b83\u4eec\u7684\u751f\u7269\u547d\u8fd0\u3002\u86cb\u767d\u8d28\u7684\u5438\u9644\u53ef\u80fd\u53d7\u7eb3\u7c73\u9897\u7c92\u624b\u6027\u8868\u9762\u7684\u5f71\u54cd\uff1b\u7136\u800c\uff0c\u7814\u7a76\u53ef\u7528\u4e8e\u86cb\u767d\u8d28\u4e0e\u7eb3\u7c73\u9897\u7c92\u7684\u624b\u6027\u8868\u9762\u7684\u76f8\u4e92\u4f5c\u7528\uff0c\u5176\u6f5c\u5728\u7684\u673a\u5236\u4ecd\u7136\u5c1a\u672a\u89e3\u51b3\u3002\u672c\u6587\u5236\u5b9a\u4e86\u4e00\u79cd\u5b9a\u91cf\u5206\u6790\u8f6c\u94c1\u86cb\u767d\u5bf9\u7528D-\u9752\u9709\u80fa\uff0cL-\u9752\u9709\u80fa\u548c\u5916\u6d88\u65cb\u9752\u9709\u80fa\u5b98\u80fd\u5316\u7684\u91d1\u7eb3\u7c73\u9897\u7c92\u7684\u5438\u9644\u548c\u6784\u8c61\u7279\u5f81\u7684\u7b56\u7565\u3002\u5229\u7528\u77f3\u82f1\u5fae\u91cf\u5929\u5e73\u5e73\u53f0\u76d1\u6d4b\u5438\u9644\u7684\u8f6c\u94c1\u86cb\u767d\u4e0eHEK\u7ec6\u80de\u884d\u751f\u8102\u8d28\u4f53\u4e2d\u8f6c\u94c1\u86cb\u767d\u53d7\u4f53\u7684\u76f8\u4e92\u4f5c\u7528\u3002\u7ed3\u679c\u8868\u660e\uff0c\u7eb3\u7c73\u9897\u7c92\u7684\u624b\u6027\u8868\u9762\u51b3\u5b9a\u4e86\u8f6c\u94c1\u86cb\u767d\u7684\u53d6\u5411\u548c\u6784\u8c61\uff0c\u5176\u968f\u540e\u5f71\u54cd\u8f6c\u8fd0\u86cb\u767d\u4e0e\u5176\u53d7\u4f53\u5728\u7ec6\u80de\u819c\u4e0a\u7684\u76f8\u4e92\u4f5c\u7528\u548c\u8bc6\u522b\u3002\u8f6c\u94c1\u86cb\u767d\u88ab\u5e7f\u6cdb\u7528\u4f5c\u764c\u75c7\u6cbb\u7597\u548c\u8bca\u65ad\u4e2d\u7684\u80bf\u7624\u9776\u5411\u914d\u4f53\uff0c\u56e0\u4e3a\u8f6c\u94c1\u86cb\u767d\u53d7\u4f53\u5728\u5404\u79cd\u7c7b\u578b\u7684\u764c\u7ec6\u80de\u7684\u7ec6\u80de\u819c\u4e0a\u8fc7\u8868\u8fbe\u3002\u56e0\u6b64\uff0c\u672c\u7ed3\u679c\u5c06\u6709\u52a9\u4e8e\u5728\u751f\u7406\u73af\u5883\u4e2d\u6269\u5927\u5177\u6709\u624b\u6027\u8868\u9762\u7684\u7eb3\u7c73\u9897\u7c92\u7684\u751f\u7269\u5b66\u8ba4\u8bc6\u7684\u77e5\u8bc6\uff0c\u5e76\u63d0\u4f9b\u5bf9\u6cbb\u7597\u6027\u7eb3\u7c73\u9897\u7c92\u7684\u5408\u7406\u8bbe\u8ba1\u7684\u6d1e\u5bdf\u3002<\/p>\n

\u3010\u6210\u679c\u7b80\u4ecb\u3011<\/strong><\/p>\n

\u8fd1\u65e5\uff0c\u56fd\u5bb6\u7eb3\u7c73\u79d1\u5b66\u4e2d\u5fc3<\/span>\u9648\u6625\u82f1<\/span><\/span>\uff08<\/strong>\u901a\u8baf\u4f5c\u8005\uff09<\/strong>\u5728ACS Nano<\/strong>\u4e0a\u53d1\u8868\u4e86\u4e00\u7bc7\u9898\u4e3a \u201cChiral Surface of Nanoparticles Determines the Orientation of Adsorbed Transferrin and Its Interaction with Receptors\u201d<\/strong>\u7684\u6587\u7ae0\u3002\u8be5\u7814\u7a76\u56e2\u961f\u5f00\u53d1\u51fa\u67e0\u6aac\u9178\u8fd8\u539fHAuCl4<\/sub>\u5408\u621014nm\u7684AuNP\uff0c\u5c06\u5177\u6709\u4e0d\u540c\u5c3a\u5bf8\u7684\u67e0\u6aac\u9178\u76d0\u4fdd\u62a4\u7684AuNP\uff08Cit-AuNP\uff09\u4e0e\u8fc7\u91cf\u7684D-Pen\u6216L-Pen\u6216D-Pen\u548cL-Pen<\/span>\u7684\u6df7\u5408\u7269\u6df7\u5408\u4e4b\u540e\u79bb\u5fc3\uff0c\u5f97\u5230\u9752\u9709\u80fa\u4fee\u9970\u7684AuNPs\uff08Pen-AuNP\uff09\u5e76\u5229\u7528\u8367\u5149\u6dec\u706d\u6d4b\u91cf\u4e0eTf\u7684\u76f8\u4e92\u4f5c\u7528\u3002\u5229\u7528\u9752\u9709\u80fa\u7684D\uff0cL\u548c\u5916\u6d88\u65cb\u5f02\u6784\u4f53\u4fee\u997013.6\u00b10.9nm AuNPs\u7684\u8868\u9762\u3002\u63a2\u8ba8\u4e86\u5236\u5907\u7684\u624b\u6027\u8868\u9762\u5bf9Tf\u5438\u9644\u7684\u5f71\u54cd\u3002\u4f7f\u7528\u4e86QCM-D\uff0c\u8367\u5149\uff0cCD\uff0cFT-IR\uff0cAFM\u548cDLS\u6280\u672f\u7b49\u6280\u672f\u89c2\u5bdf\u70ed\u529b\u5b66\uff0c\u5438\u9644\u65b9\u5411\u548c\u7ed3\u6784\u7279\u5f81\u4e4b\u95f4\u7684\u5fae\u5999\u5dee\u5f02\u3002\u540c\u65f6\u4f7f\u7528\u77f3\u82f1\u5fae\u91cf\u5929\u5e73\u5e73\u53f0\u6765\u6d4b\u91cf\u5438\u9644\u5728AuNPs\u7684\u624b\u6027\u8868\u9762\u4e0a\u7684Tf\u548c\u5728HEK293\u7ec6\u80de\u884d\u751f\u7684\u8102\u8d28\u4f53\u4e2d\u8868\u8fbe\u7684TfR\u7684\u76f8\u4e92\u4f5c\u7528\u3002<\/p>\n

[\u6ce8\uff1aTf- Transferrin \u8f6c\u94c1\u86cb\u767d\uff1bD-Pen\u4e3a<\/span>\u00a0D-\u9752\u9709\u80fa\u00a0\u00a0L<\/span>-Pen \u4e3aL-\u9752\u9709\u80fa\u00a0 ]<\/span><\/strong><\/p>\n

\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/strong><\/p>\n

\u56fe<\/strong>1\u00a0<\/strong>\u624b\u6027\u5206\u5b50\u5bf9<\/strong>AuNP<\/strong>\u8868\u9762\u7684\u4fee\u9970\u53ca\u5176\u4e0e<\/strong>Tf<\/strong>\u7684\u76f8\u4e92\u4f5c\u7528\u7684\u793a\u610f\u56fe<\/strong><\/p>\n

\"\"<\/p>\n

\u624b\u6027\u5206\u5b50\u5bf9AuNP\u8868\u9762\u7684\u4fee\u9970\u53ca\u5176\u4e0eTf\u7684\u76f8\u4e92\u4f5c\u7528\u7684\u793a\u610f\u56fe\u3002<\/p>\n

\u56fe<\/strong>2 Pen-AuNPs<\/strong>\u4e0a<\/strong>Tf<\/strong>\u7535\u6655\u5f62\u6210\u7684\u5b9a\u91cf\u5206\u6790<\/strong><\/p>\n

\"\"<\/p>\n

\uff08a\uff09AuNP\uff08D\uff09-Tf\uff08a\uff09\u7684\u6d41\u4f53\u52a8\u529b\u5b66\u534a\u5f84\u6d4b\u91cf\u503c\u4f5c\u4e3a\u6d53\u5ea6\u7684\u51fd\u6570\u7684Tf\uff1b<\/p>\n

\uff08b\uff09AuNP\uff08L\uff09-Tf\uff08b\uff09\u7684\u6d41\u4f53\u52a8\u529b\u5b66\u534a\u5f84\u6d4b\u91cf\u503c\u4f5c\u4e3a\u6d53\u5ea6\u7684\u51fd\u6570\u7684Tf\uff1b<\/p>\n

\uff08c\uff09AuNP\uff08D\/L\uff09-Tf\uff08c\uff09\u7684\u6d41\u4f53\u52a8\u529b\u5b66\u534a\u5f84\u6d4b\u91cf\u503c\u4f5c\u4e3a\u6d53\u5ea6\u7684\u51fd\u6570\u7684Tf\uff1b<\/p>\n

\uff08d\uff09\u5728Tf\u7684\u516d\u4e2a\u4fa7\u9762\u4e0a\u5177\u6709\u7535\u8377\u548c\u7ed3\u5408-TfR\u76f8\u5173\u6b8b\u57fa\u7684\u6c28\u57fa\u9178\u7684\u5206\u5e03\u3002\u4f7f\u7528\u5177\u6709\u5f69\u8272\u7f16\u7801\u7c7b\u578b\u7684\u7535\u8377\uff08\u7ea2\u8272\uff0c\u8d1f\u7535\u8377;\u84dd\u8272\uff0c\u6b63\u7535\u8377;\u7070\u8272\uff0c\u65e0\u7535\u8377\uff09\u7684Tf\uff08PDB ID\uff1a4X1B\uff09\u7684\u7a7a\u95f4\u586b\u5145\u6a21\u578b\u63cf\u8ff0\u4e86Tf\u76843D\u7ed3\u6784\u3002\u9ec4\u8272\u6807\u8bb0\u7684\u6b8b\u57fa\u4ee3\u8868Tf\u7684C\u6ce2\u548cN\u6ce2\u7684\u7ed3\u5408TfR\u76f8\u5173\u6b8b\u57fa\uff1b<\/p>\n

\uff08e\uff09Tf\u865a\u62df\u7acb\u65b9\u4f53\u6bcf\u4fa7\u7684\u5e26\u7535\u6c28\u57fa\u9178\u7684\u7edf\u8ba1\u5206\u5e03\uff1b<\/p>\n

\uff08f\uff09\u7531QCM-D\u76d1\u6d4b\u7684\u5438\u9644\u5728Pen-AuNP\u548cSLB\u4e0a\u7684Tf\uff08POPC:POEPC\uff0c8:2\uff09\u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u793a\u610f\u56fe\uff1b<\/p>\n

\uff08g\uff09\u5b75\u80b23\u5c0f\u65f6\u540e\uff0c\u5c06\u5177\u67091\u03bcm\u626b\u63cf\u5c3a\u5bf8\u7684Tf\u5438\u9644\u7684Pen-AuNP\uff08150\u03bcg\/mL\uff09\u5438\u9644\u5230POPC\/POEPC\u53cc\u5c42\u4e0a\u7684AFM\u6210\u50cf\uff1b<\/p>\n

\uff08h\uff09\u5c06Tf\u5438\u9644\u7684Pen-AuNP\u5438\u9644\u5230SLB\u4e0a\u7684QCM-D\u7ed3\u679c\u3002<\/p>\n

\u56fe<\/strong>3 Pen-AuNPs<\/strong>\u76f8\u4e92\u4f5c\u7528\u524d\u540e<\/strong>Tf<\/strong>\u4e8c\u7ea7\u7ed3\u6784\u7684<\/strong>CD<\/strong>\u548c<\/strong>FTIR<\/strong>\u5206\u6790<\/strong><\/p>\n

\"\"<\/p>\n

\uff08a\uff09\u4e0ePen-AuNPs\u76f8\u4e92\u4f5c\u7528\u524d\u540eTf\u7684\u4e8c\u7ea7\u7ed3\u6784\u7684CD\u5149\u8c31\uff1b<\/p>\n

\uff08b\uff09\u5728\u86cb\u767d\u8d28\u4e8c\u7ea7\u7ed3\u6784\u7814\u7a76\u4e2d\u9009\u62e9\u7684\u539f\u59cb\u8c31\uff082000-1300com-1<\/sup>\uff09\u4f5c\u4e3a\u7814\u7a76\u7684\u533a\u57df\uff1b<\/p>\n

\uff08c-g\uff09\u5438\u9644\u5728\uff08b\uff09\u7684AuNPs\u7684\u624b\u6027\u8868\u9762\u4e0a\u7684Tf\u7684\u9170\u80faI\u5149\u8c31\u7684\u66f2\u7ebf\u62df\u5408\u5012\u7f6eSD\u548c\u66f2\u7ebf\u62df\u5408\u7684\u5012\u7f6eFSD\uff0c\u66f2\u7ebf\u62df\u5408\u4f7f\u7528OMNIC\u8f6f\u4ef6\u8fdb\u884c\u3002<\/p>\n

\u56fe<\/strong>4\u00a0<\/strong>\u7eb3\u7c73\u624b\u6027\u8868\u9762\u5bf9<\/strong>Tf-TfR<\/strong>\u76f8\u4e92\u4f5c\u7528\u7684\u5f71\u54cd<\/strong><\/p>\n

\"\"<\/p>\n

\uff08a\uff09\u5b9e\u9a8c\u8bbe\u8ba1\u539f\u7406\u603b\u4f53\u6846\u67b6\u56fe\uff1b<\/p>\n

\uff08b\uff09COOH\u5305\u88ab\u7684\u82af\u7247\u88abEDC\/NHS\u6fc0\u6d3b\u540e\uff0c\u5c06\u82af\u7247\u4f9d\u6b21\u66b4\u9732\u4e8ePen-AuNP\uff08\u6b65\u9aa41\uff09;<\/p>\n

\uff08c\uff09\uff08b\uff09\uff08a\uff09\u4e2dQCM-D\u6d4b\u91cf\u7684\u9891\u7387\u53d8\u5316\uff08\u0394F\uff09\u7684\u793a\u610f\u56fe\uff1b<\/p>\n

\uff08d\uff09\u6b65\u9aa43\u7684\u9891\u7387\u53d8\u5316\u76f4\u65b9\u56fe\uff0c\u5373\u5438\u9644\u5230Pen-AuNP\u4e0a\u7684Tf\u548c\u5728\u7ec6\u80de\u6765\u6e90\u7684\u8102\u8d28\u4f53\u4e0a\u8868\u8fbe\u7684TfR\u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u3002<\/p>\n

\u3010\u5c0f\u7ed3\u3011<\/strong><\/p>\n

\u8be5\u7814\u7a76\u56e2\u961f\u901a\u8fc7\u624b\u6027\u8868\u9762\u7684NPs\u5bf9Tf\u548cTfR\u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u7684\u5f71\u54cd\u53ef\u4ee5\u4e0eTf\u5728\u624b\u6027\u7eb3\u7c73\u754c\u9762\u7684\u5438\u9644\u884c\u4e3a\u76f8\u5173\u3002Tf\u572814nm NPs\u7684\u4e0d\u540c\u624b\u6027\u8868\u9762\u4e0a\u7684\u5438\u9644\u4e0d\u4f1a\u53d1\u751f\u81ea\u53d1\u548c\u653e\u70ed\u8fc7\u7a0b\u3002\u5438\u9644\u5728AuNPs\u4e0d\u540c\u624b\u6027\u8868\u9762\u4e0a\u7684Tf\u7684\u5dee\u5f02\u4f53\u73b0\u5728\u5438\u9644\u53d6\u5411\u548c\u6784\u8c61\u53d8\u5316\u4e2d\u3002\u540c\u65f6\uff0cTf\u5728\u4e0eAuNPs\u7684\u4e0d\u540c\u624b\u6027\u8868\u9762\u76f8\u4e92\u4f5c\u7528\u540e\uff0c\u4e8c\u6b21\u7ed3\u6784\u53d1\u751f\u4e0d\u540c\u7a0b\u5ea6\u7684\u53d8\u5316\u3002\u6b64\u5916\uff0c\u7814\u7a76\u8868\u660e\uff1aNPs\u7684\u8868\u9762\u66f2\u7387\u5bf9\u624b\u6027\u7eb3\u7c73\u754c\u9762\u7684\u86cb\u767d\u8d28\u7684\u5438\u9644\u53d6\u5411\u548c\u5c42\u539a\u5ea6\u6709\u663e\u7740\u7684\u5f71\u54cd\u3002\u52a0\u6df1\u5bf9\u751f\u7406\u73af\u5883\u4e2d\u5177\u6709\u624b\u6027\u8868\u9762NPs\u7684\u751f\u7269\u5b66\u7279\u5f81\u7684\u7406\u89e3\u3002<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1a<\/strong>Chiral Surface of Nanoparticles Determines the Orientation of Adsorbed Transferrin and Its Interaction with Receptors<\/a>\uff08ACS Nano, 2017, DOI: 10.1021\/acsnano.7b00200\uff09<\/p>\n

\u901a\u8baf\u4f5c\u8005\u7b80\u4ecb\uff1a<\/strong><\/p>\n

\u9648\u6625\u82f1\uff0c\u56fd\u5bb6\u7eb3\u7c73\u79d1\u5b66\u4e2d\u5fc3\u7814\u7a76\u5458\u3001\u535a\u58eb\u751f\u5bfc\u5e08\u30021996\u5e74\u83b7\u5f97\u534e\u4e2d\u7406\u5de5\u5927\u5b66\u751f\u7269\u533b\u5b66\u5de5\u7a0b\u4e13\u4e1a\u533b\u5b66\u535a\u58eb\u5b66\u4f4d\u30021996\u5e7411\u6708\u81f32006\u5e745\u6708\uff0c\u4e2d\u56fd\u79d1\u5b66\u9662\u9ad8\u80fd\u7269\u7406\u7814\u7a76\u6240\u535a\u58eb\u540e\u5e76\u7559\u6240\u5de5\u4f5c\uff1b2001\u5e749\u6708-2002\u5e749\u6708\uff0c\u745e\u5178\u5361\u7f57\u6797\u65af\u5361\u5927\u5b66\u8bfa\u8d1d\u5c14\u533b\u5b66\u751f\u7269\u5316\u5b66\u7814\u7a76\u6240\u535a\u58eb\u540e\u30022006\u5e746\u6708\u52a0\u5165\u56fd\u5bb6\u7eb3\u7c73\u79d1\u5b66\u4e2d\u5fc3\u3002\u5148\u540e\u4e3b\u6301\u79d1\u6280\u90e8973\u9879\u76ee\u3001\u56fd\u5bb6\u4eea\u5668\u4e13\u9879\u3001\u56fd\u5bb6\u81ea\u7136\u79d1\u5b66\u57fa\u91d1\u3001\u6b27\u76df\u7b2c\u516d\u3001\u7b2c\u4e03\u6846\u67b6\u8ba1\u5212(EU-FP6&FP7)\u3001\u56fd\u9645\u539f\u5b50\u80fd\u673a\u6784\u534f\u8c03\u7814\u7a76\u8ba1\u5212(IAEA)\u7b49\u591a\u9879\u56fd\u5185\u4e0e\u56fd\u9645\u5408\u4f5c\u9879\u76ee\u3002\u4e2d\u56fd\u6bd2\u7406\u5b66\u4f1a\u53ca\u56fd\u9645\u7eaf\u7cb9\u4e0e\u5e94\u7528\u5316\u5b66\u8054\u5408\u4f1a\u5458\uff0c\u62c5\u4efbMetallomics\u3001Nanotoxicology\u3001Particle and Fibre Toxicology\u548cCurrent Drug Metabolism\u7f16\u59d4\u3002\u76f8\u5173<\/span>\u7814\u7a76\u6210\u679c\u5df2\u5728Nat Methods, Nat Commun, Chem Soc Rev, Acc Chem Res, PNAS, Nano Lett, Adv Mater, ACS Nano, Small, Biomaterials\u7b49\u56fd\u9645\u8457\u540d\u520a\u7269\u53d1\u8868\u5b66\u672f\u8bba\u6587150\u4f59\u7bc7\u3002\u4e2d\u56fd\u6388\u6743\u4e13\u522914\u9879\uff0c\u56fd\u9645\u6388\u6743\u4e13\u52291\u9879\u30022008\u5e74\u83b7\u201c\u5317\u4eac\u5e02\u79d1\u5b66\u6280\u672f\u4e8c\u7b49\u5956(\u7b2c\u4e8c\u83b7\u5956\u4eba)\u201d\uff0c2011\u5e74\u83b7\u201c\u4e2d\u56fd\u6807\u51c6\u5316\u6770\u51fa\u4eba\u7269\u2014\u521b\u65b0\u4eba\u7269\u201d\u5956\uff0c2012\u5e74\u83b7\u201c\u56fd\u5bb6\u81ea\u7136\u79d1\u5b66\u4e8c\u7b49\u5956(\u7b2c\u4e8c\u83b7\u5956\u4eba)\u201d\uff0c2014\u5e74\u83b7\u201c\u56fd\u5bb6\u6770\u51fa\u9752\u5e74\u79d1\u5b66\u57fa\u91d1\u201d\u8d44\u52a9\u4ee5\u53ca\u201c\u4e2d\u56fd\u9752\u5e74\u5973\u79d1\u5b66\u5bb6\u5956\u201d\uff0c\u5e76\u5165\u9009\u201c\u56fd\u5bb6\u767e\u5343\u4e07\u4eba\u624d\u5de5\u7a0b\u201d\u3001Thomson Reuters\u516c\u5e03\u7684\u201c2014\u5e74\u5168\u7403\u9ad8\u5f15\u7528\u79d1\u5b66\u5bb6\u201d\u4ee5\u53ca\u201c\u4e2d\u79d1\u9662\u767e\u4eba\u8ba1\u5212\u201d\u3002\u76ee\u524d\u4e3b\u8981\u7814\u7a76\u65b9\u5411\uff1a\uff081\uff09\u9ad8\u6548\u4f4e\u6bd2\u6297\u80bf\u7624\u7eb3\u7c73\u836f\u7269\u7684\u7814\u5236\u53ca\u5176\u4f5c\u7528\u673a\u5236\u7684\u7814\u7a76\uff1b\uff082\uff09\u5178\u578b\u7eb3\u7c73\u6750\u6599\u4e0e\u751f\u7269\u4f53\u76f8\u4e92\u4f5c\u7528\u7684\u89c4\u5f8b\u53ca\u5176\u5f71\u54cd\u56e0\u7d20\uff1b\uff083\uff09\u6838\u5206\u6790\u4e0e\u540c\u6b65\u8f90\u5c04\u6280\u672f\u53ca\u7ec4\u5b66\u6280\u672f\u7528\u4e8e\u751f\u7269\u4f53\u7cfb\u7eb3\u7c73\u9897\u7c92\u66b4\u9732\u548c\u6548\u5e94\u6807\u5fd7\u7269\u7684\u7814\u7a76\uff1b\uff084\uff09\u6613\u611f\u4eba\u7fa4\u73af\u5883\u6c61\u67d3\u7269\u957f\u671f\u66b4\u9732\u7684\u5206\u5b50\u6bd2\u7406\u5b66\u7814\u7a76\u3002<\/p>\n

\u672c\u6587\u7531\u6750\u6599\u4eba\u7f16\u8f91\u90e8\u7eb3\u7c73\u6750\u6599\u5b66\u672f\u7ec4Kevin\u4f9b\u7a3f\uff0c\u6750\u6599\u725b\u7f16\u8f91\u6574\u7406<\/strong>\u3002\u6b22\u8fce\u52a0\u5165\u6750\u6599\u4eba\u7eb3\u7c73\u5b66\u672f\u4ea4\u6d41\u7fa4\uff08228686798\uff09\uff01<\/p>\n

\u6750\u6599\u725b\u7f51\u4e13\u6ce8\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u53ca\u884c\u4e1a\u8fdb\u5c55\uff0c\u8fd9\u91cc\u6c47\u96c6\u4e86\u5404\u5927\u9ad8\u6821\u7855\u535a\u751f\u3001\u4e00\u7ebf\u79d1\u7814\u4eba\u5458\u4ee5\u53ca\u884c\u4e1a\u4ece\u4e1a\u8005\uff0c\u5982\u679c\u60a8\u5bf9\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u8fdb\u5c55\uff0c\u89e3\u8bfb\u9ad8\u6c34\u5e73\u6587\u7ae0\u6216\u662f\u8bc4\u8ff0\u884c\u4e1a\u6709\u5174\u8da3\uff0c\u70b9\u6211\u52a0\u5165\u6750\u6599\u4eba\u7f16\u8f91\u90e8<\/a>\u3002<\/p>\n

\u6750\u6599\u6d4b\u8bd5\uff0c\u6570\u636e\u5206\u6790<\/strong>\uff0c\u4e0a\u6d4b\u8bd5\u8c37<\/a>\uff01<\/p>\n","protected":false},"excerpt":{"rendered":"

\u3010\u5f15\u8a00\u3011 \u7eb3\u7c73\u9897\u7c92\u66b4\u9732\u751f\u7406\u73af\u5883\u65f6\uff0c\u5f62\u6210\u201c\u86cb\u767d\u8d28\u7535\u6655\u201d\uff0c\u5176\u6781\u5927\u5730\u51b3\u5b9a\u4e86\u5b83\u4eec\u7684\u751f\u7269\u547d\u8fd0\u3002\u86cb\u767d\u8d28\u7684\u5438\u9644\u53ef\u80fd\u53d7\u7eb3\u7c73\u9897\u7c92\u624b\u6027\u8868\u9762\u7684\u5f71\u54cd\uff1b\u7136\u800c\uff0c\u7814\u7a76\u53ef\u7528\u4e8e\u86cb\u767d\u8d28\u4e0e\u7eb3\u7c73\u9897\u7c92\u7684…<\/p>\n","protected":false},"author":3440,"featured_media":81036,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[215],"tags":[54,981],"class_list":["post-81023","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-jiedu","tag-making","tag-test"],"_links":{"self":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/81023"}],"collection":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/users\/3440"}],"replies":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=81023"}],"version-history":[{"count":14,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/81023\/revisions"}],"predecessor-version":[{"id":81612,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/81023\/revisions\/81612"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/media\/81036"}],"wp:attachment":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=81023"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=81023"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=81023"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}