{"id":78186,"date":"2017-04-28T13:40:08","date_gmt":"2017-04-28T05:40:08","guid":{"rendered":"\/\/m.iemloyee.com\/?p=78186"},"modified":"2017-04-28T13:42:21","modified_gmt":"2017-04-28T05:42:21","slug":"%e6%b5%8b%e8%af%95%e5%b9%b2%e8%b4%a7%e5%86%b7%e5%86%bb%e6%89%ab%e6%8f%8f%e7%94%b5%e9%95%9c%e6%8a%80%e6%9c%af%e7%9a%84%e5%ba%94%e7%94%a8","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=78186","title":{"rendered":"\u6d4b\u8bd5\u5e72\u8d27|\u51b7\u51bb\u626b\u63cf\u7535\u955c\u6280\u672f\u7684\u5e94\u7528"},"content":{"rendered":"<p><strong> 1. \u51b7\u51bb\u626b\u63cf\u7535\u955c\u6982\u8ff0<\/strong><\/p>\n<p>\u5e38\u89c4\u626b\u63cf\u7535\u955c\u8981\u6c42\u6240\u89c2\u5bdf\u7684\u6837\u54c1\u65e0\u6c34\uff0c\u800c\u4e00\u4e9b\u6837\u54c1\u5728\u5e72\u71e5\u8fc7\u7a0b\u4e2d\u4f1a\u53d1\u751f\u7ed3\u6784\u53d8\u5316\uff0c\u81f4\u4f7f\u65e0\u6cd5\u89c2\u5bdf\u5176\u771f\u5b9e\u7ed3\u6784\u3002\u51b7\u51bb\u626b\u63cf\u7535\u955c\u6280\u672f\u80fd\u591f\u5bf9\u542b\u6c34(\u6eb6\u5242)\u6837\u54c1\u8fdb\u884c\u6210\u50cf\u548c\u5206\u6790\uff0c\u5b9e\u73b0\u5bf9\u4e8e\u6db2\u6001\u6837\u54c1(\u5982\u4e73\u6db2\u3001\u56ca\u6ce1\u7b49)\u53ca\u4e0d\u8010\u7535\u5b50\u675f\u635f\u4f24\u6837\u54c1\u7684\u5fae\u89c2\u5f62\u8c8c\u7684\u89c2\u5bdf\u3002\u5728\u51b7\u51bb\u626b\u63cf\u7535\u955c\u89c2\u5bdf\u524d\uff0c\u6db2\u6001\u6837\u54c1\u7ecf\u8fc7\u4f4e\u6e29\u51b7\u51bb\u56fa\u5b9a\u3001\u65ad\u88c2\u3001\u9540\u819c(\u55b7\u91d1\/\u55b7\u78b3)\u7b49\u5236\u6837\u5904\u7406\u540e\uff0c\u518d\u901a\u8fc7\u51b7\u51bb\u4f20\u8f93\u7cfb\u7edf\u5c06\u5176\u7f6e\u4e8e\u7535\u955c\u5185\u7684\u51b7\u53f0\uff08\u6e29\u5ea6\u53ef\u81f3-185\u2103\u5373\u53ef\u8fdb\u884c\u89c2\u5bdf\u3002\u5176\u4e2d\uff0c\u5feb\u901f\u51b7\u51bb\u6280\u672f\u53ef\u4f7f\u6c34\u5728\u4f4e\u6e29\u72b6\u6001\u4e0b\u5448\u73bb\u7483\u6001\uff0c\u51cf\u5c11\u51b0\u6676\u7684\u4ea7\u751f\uff0c\u4ece\u800c\u4e0d\u5f71\u54cd\u6837\u54c1\u672c\u8eab\u7ed3\u6784\uff0c\u51b7\u51bb\u4f20\u8f93\u7cfb\u7edf\u4fdd\u8bc1\u6837\u54c1\u4f20\u8f93\u8fc7\u7a0b\u4e2d\u4fdd\u6301\u4f4e\u6e29\u72b6\u6001\uff0c\u6574\u4e2a\u7cfb\u7edf\u4ece\u5236\u6837\u5230\u89c2\u5bdf\u90fd\u5728\u4f4e\u6e29\u4e0b\u8fdb\u884c\uff0c\u5c3d\u53ef\u80fd\u4fdd\u6301\u6837\u54c1\u5728\u6db2\u76f8\u4e2d\u7684\u539f\u4f4d\u7ed3\u6784\u3002<\/p>\n<p><strong> 2. \u7cfb\u7edf\u7ec4\u6210\u53ca\u6d41\u7a0b<\/strong><\/p>\n<p>\u51b7\u51bb\u626b\u63cf\u7535\u955c\u7cfb\u7edf\u5305\u62ec\u51b7\u51bb\u56fa\u5b9a\u3001\u51b7\u51bb\u65ad\u88c2\u3001\u5347\u534e\u53ca\u5bfc\u7535\u6027\u55b7\u6d82\u7b49\u5236\u6837\u8bbe\u5907\uff0c\u51b7\u51bb\u4f20\u8f93\u88c5\u7f6e\u53ca\u626b\u63cf\u7535\u955c\u7684\u51b7\u53f0\uff0c\u6837\u54c1\u9996\u5148\u7ecf\u8fc7\u9ad8\u538b\u51b7\u51bb\u6216\u6db2\u6c2e\u6ce5\u65b9\u6cd5\u8fdb\u884c\u51b7\u51bb\u56fa\u5b9a\uff0c\u7136\u540e\u5728\u51b7\u51bb\u6761\u4ef6\u4e0b\u4f20\u8f93\u5230\u771f\u7a7a\u6e85\u5c04\u4eea\u4e2d\u8fdb\u884c\u65ad\u88c2\uff0c\u9732\u51fa\u6837\u54c1\u65b0\u9c9c\u7684\u65ad\u88c2\u9762\uff0c\u5e76\u6839\u636e\u6837\u54c1\u60c5\u51b5\u8fdb\u884c\u5347\u534e\u9632\u6b62\u8868\u9762\u88ab\u51b0\u5305\u8986\uff0c\u4e4b\u540e\u8fdb\u884c\u5bfc\u7535\u6027\u55b7\u6d82\uff0c\u81f3\u6b64\u5236\u6837\u5b8c\u6210\uff0c\u901a\u8fc7\u51b7\u51bb\u4f20\u8f93\u7cfb\u7edf\u5c06\u6837\u54c1\u7f6e\u4e8e\u626b\u63cf\u7535\u955c\u7684\u51b7\u53f0\u8fdb\u884c\u89c2\u5bdf\u3002\u5206\u6790\u6d4b\u8bd5\u4e2d\u5fc3\u7535\u955c\u7ec4\u73b0\u6709\u7684\u51b7\u51bb\u626b\u63cf\u7535\u955c\u7cfb\u7edf\u5305\u62ec\u9ad8\u538b\u51b7\u51bb\u4eea Leica EM HPM100\u3001\u51b7\u51bb\u4f20\u8f93\u6837\u54c1\u6746 Leica EM VCT100\u3001\u79bb\u5b50\u6e85\u5c04\u4eea Leica EM ACE600\u3001\u81ea\u5236\u7684\u6db2\u6c2e\u6ce5\u51b7\u51bb\u88c5\u7f6e\u3001\u51b7\u51bb\u626b\u63cf\u7535\u955c\u7528\u7684\u51b7\u53f0\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-78187 size-full\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/1-12.png\" alt=\"\" width=\"1129\" height=\"432\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/1-12.png 1129w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/1-12-300x115.png 300w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/1-12-768x294.png 768w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/1-12-1024x392.png 1024w\" sizes=\"(max-width: 1129px) 100vw, 1129px\" \/><\/p>\n<p>\u9ad8\u538b\u51b7\u51bb\u4eea<br \/>\n Leica EM HPM100Leica \u00a0 \u00a0 \u51b7\u51bb\u4f20\u8f93\u6837\u54c1\u6746 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0\u79bb\u5b50\u6e85\u5c04\u4eea \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u51b7\u51bb\u626b\u63cf\u7535\u955c\u51b7\u53f0<br \/>\n \u6db2\u6c2e\u6ce5\u51b7\u51bb\u88c5\u7f6e \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0Leica EM VCT100 \u00a0 \u00a0 \u00a0Leica EM ACE600<br \/>\n Nitrogen slush freezer<\/p>\n<p><strong> 2.1 \u51b7\u51bb\u56fa\u5b9a<\/strong><\/p>\n<p><strong>2.1.1 \u9ad8\u538b\u51b7\u51bb\u56fa\u5b9a<\/strong><\/p>\n<p>\u51b7\u51bb\u56fa\u5b9a\u7684\u76ee\u6807\u662f\u5728\u4f4e\u6e29\u4e0b\u4f7f\u6db2\u6001\u6837\u54c1\u4e2d\u7684\u6c34\u4e0d\u7ed3\u6676\u800c\u5448\u73bb\u7483\u6001\uff0c\u8fd9\u6837\u65e2\u80fd\u5c06\u6837\u54c1\u5728\u6db2\u76f8\u7684\u72b6\u6001\u56fa\u5b9a\uff0c\u53c8\u907f\u514d\u4e86\u6c34\u7ed3\u6676\u5f15\u8d77\u6837\u54c1\u7ed3\u6784\u7684\u7834\u574f\u3002\u56e0\u6b64\uff0c\u51b7\u51bb\u56fa\u5b9a\u7684\u5173\u952e\u662f\u8981\u963b\u6b62\u51b0\u6676\u5f62\u6210\uff0c\u51b7\u51bb\u56fa\u5b9a\u662f\u5426\u6210\u529f\uff0c\u53d6\u51b3\u4e8e\u51b7\u51bb\u8fc7\u7a0b\u8fc5\u901f\u901a\u8fc7\u4e00\u4e2a\u6e29\u5ea6\u8303\u56f4\uff0c\u5373\u6c34\u5f00\u59cb\u7ed3\u6676\u7684\u6e29\u5ea6\u5230\u5f00\u59cb\u91cd\u7ed3\u6676\u7684\u6e29\u5ea6\u533a\u95f4\uff0c\u6b64\u533a\u95f4\u53d7\u73af\u5883\u538b\u529b\u3001\u6837\u54c1\u6d53\u5ea6\u7b49\u56e0\u7d20\u7684\u5f71\u54cd\u3002\u5982\u56fe 1<sup>[1]<\/sup>\u6240\u793a\uff0c\u7b2c\u4e00\u884c\u662f\u5e38\u538b\u4e0b\u7684\u7eaf\u6c34\uff0c\u4ece 273 K \u5f00\u59cb\u7ed3\u6676\uff0c\u56e0\u8003\u8651\u5230\u8fc7\u51b7\u6c34\u7684\u4f5c\u7528\uff0c\u6240\u4ee5\u4ece 231 K \u5f00\u59cb\u7ed3\u6676\uff0c\u5f53\u6e29\u5ea6\u4f4e\u4e8e\u91cd\u7ed3\u6676\u6e29\u5ea6 136 K \u65f6\uff0c\u7ed3\u6676\u8fc7\u7a0b\u505c\u6b62\u3002\u7b2c\u4e8c\u884c\u662f\u5e38\u538b\u4e0b\u7684\u6837\u54c1\uff0c\u901a\u5e38\u6837\u54c1\u5305\u542b\u6eb6\u8d28\u4f1a\u964d\u4f4e\u6837\u54c1\u7684\u7194\u70b9\uff08\u4e0e\u6eb6\u8d28\u6d53\u5ea6\u6709\u5173\u7cfb\uff09\uff0c\u540c\u65f6\u63d0\u9ad8\u91cd\u7ed3\u6676\u6e29\u5ea6\uff0c\u4f7f\u5f97\u7ed3\u6676\u7684\u6e29\u5ea6\u533a\u95f4\u51cf\u5c0f\u3002\u7b2c\u4e09\u884c\u662f\u65bd\u52a0 204.5 MPa \u538b\u529b\u7684\u6837\u54c1\uff0c\u53ef\u4ee5\u770b\u51fa\uff0c\u65bd\u52a0\u9ad8\u538b\u4f1a\u8fdb\u4e00\u6b65\u964d\u4f4e\u6837\u54c1\u7684\u7194\u70b9\u4e14\u63d0\u9ad8\u91cd\u7ed3\u6676\u7684\uff0c\u6240\u4ee5\u7ed3\u6676\u7684\u6e29\u5ea6\u533a\u95f4\u8fdb\u4e00\u6b65\u51cf\u5c0f\u3002<\/p>\n<p>\u56e0\u6b64\uff0c\u53ef\u7528\u9ad8\u538b\u51b7\u51bb\u7684\u65b9\u5f0f\u5c06\u6db2\u6001\u6837\u54c1\u8fdb\u884c\u5feb\u901f\u51b7\u51bb\u56fa\u5b9a\uff0c\u5177\u4f53\u5982\u56fe 2<sup>[1]<\/sup>\u6240\u793a\uff0c\u5728 10ms \u5185\uff0c\u538b\u529b\u7531\u5e38\u538b\u77ac\u95f4\u5347\u9ad8\u81f3\u7ea6 200 MPa\uff0c\u540c\u65f6\u7528\u6db2\u6c2e\u4f7f\u6837\u54c1\u6e29\u5ea6\u8fc5\u901f\u4e0b\u964d\uff0c\u5f53\u901a\u8fc7\u6c34\u7684\u7ed3\u6676\u533a\u95f4\uff0c\u76f4\u5230\u6e29\u5ea6\u4f4e\u4e8e\u7ea6 136 K \u540e\uff0c\u5373\u5f62\u6210\u73bb\u7483\u6001\u7684\u6c34\u3002\u5bf9\u4e8e 200 \u00b5m <sup>[3]<\/sup>\u539a\u7684\u6837\u54c1\uff0c\u6837\u54c1\u4e2d\u5fc3\u533a\u57df\u901a\u8fc7\u8fd9\u4e00\u6e29\u5ea6\u533a\u95f4\u5927\u7ea6\u9700\u8981 30 ms\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-78188 size-full\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/2-13.png\" alt=\"\" width=\"830\" height=\"327\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/2-13.png 830w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/2-13-300x118.png 300w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/2-13-768x303.png 768w\" sizes=\"(max-width: 830px) 100vw, 830px\" \/><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-78189 size-full\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/3-12.png\" alt=\"\" width=\"900\" height=\"658\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/3-12.png 900w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/3-12-300x219.png 300w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/3-12-768x561.png 768w\" sizes=\"(max-width: 900px) 100vw, 900px\" \/><\/p>\n<p>\u5177\u4f53\u64cd\u4f5c\u6d41\u7a0b\u5982\u4e0b\uff0c\u7528\u79fb\u6db2\u67aa\u5c06\u5f85\u6d4b\u6837\u54c1\u6ef4\u5165\u51b7\u51bb\u6837\u54c1\u5939\u7247\u7684\u4e0b\u7247\uff0c\u4e0b\u7247\u4e2d\u6709\u51f9\u69fd\uff0c\u53ef\u50a8\u5b58\u4e00\u5b9a\u91cf\u7684\u6db2\u4f53\u6837\u54c1\uff0c\u7136\u540e\u76d6\u4e0a\u5939\u7247\u4e0a\u7247\uff0c\u7ec4\u6210\u4e00\u4e2a\u4e09\u660e\u6cbb\u7ed3\u6784\uff0c\u4e0a\u4e0b\u4e24\u7247\u7684\u6750\u8d28\u5747\u4e3a\u94dc\u9540\u91d1\uff0c\u4e4b\u540e\u5c06\u8f7d\u6709\u6837\u54c1\u7684\u6574\u4e2a\u5939\u7247\u7f6e\u4e8e\u7eff\u8272\u7684\u56fa\u5b9a\u7247\u4e0a\uff0c\u63a8\u5165\u9ad8\u538b\u51b7\u51bb\u4eea\u4e2d\u5373\u53ef\u8fdb\u884c\u9ad8\u538b\u51b7\u51bb\u56fa\u5b9a\uff08\u56fe 3\uff09\uff0c\u6bcf\u6b21\u8fdb\u884c\u4e00\u6b21\u9ad8\u538b\u51b7\u51bb\uff0c\u4eea\u5668\u4f1a\u751f\u6210\u4e00\u6761\u51b7\u51bb\u66f2\u7ebf\uff0c\u6839\u636e\u66f2\u7ebf\u4e2d\u8fbe\u5230\u76ee\u6807\u538b\u529b\u548c\u6e29\u5ea6\u7684\u65f6\u95f4\u53ef\u521d\u6b65\u5224\u65ad\u51b7\u51bb\u56fa\u5b9a\u7684\u6548\u679c\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-78190 size-full\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/4-11.png\" alt=\"\" width=\"1027\" height=\"519\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/4-11.png 1027w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/4-11-300x152.png 300w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/4-11-768x388.png 768w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/4-11-1024x517.png 1024w\" sizes=\"(max-width: 1027px) 100vw, 1027px\" \/><\/p>\n<p><strong> 2.1.2 \u6db2\u6c2e\u6ce5\u51b7\u51bb\u56fa\u5b9a<\/strong><\/p>\n<p>\u9ad8\u538b\u51b7\u51bb\u56fa\u5b9a\u7684\u65b9\u6cd5\u867d\u7136\u80fd\u8f83\u597d\u7684\u4f7f\u6c34\u56fa\u5b9a\u5728\u73bb\u7483\u6001\uff0c\u4f46\u662f\u9ad8\u538b\u4f1a\u5f71\u54cd\u67d0\u4e9b\u6837\u54c1\u7684\u5fae\u89c2\u7ed3\u6784\uff0c\u800c\u4e14\u9ad8\u538b\u51b7\u51bb\u4eea\u4ef7\u683c\u6602\u8d35\u3002\u8fd8\u53ef\u5e94\u7528\u6db2\u6c2e\u65b9\u5f0f\u8fdb\u884c\u51b7\u51bb\u56fa\u5b9a\uff0c\u5373\u5c06\u6db2\u6c2e\u7f6e\u4e8e\u771f\u7a7a\u4e2d\uff0c\u5728\u4e00\u5b9a\u7684\u8d1f\u538b\u4e0b\uff08\u901a\u5e38200Pa\uff09\u00a0<sup>[3]<\/sup>\uff0c\u6db2\u6c2e\u7531\u6db2\u6001\u9010\u6e10\u53d8\u4e3a\u56fa\u6001\uff0c\u5f62\u6210\u6db2\u6c2e\u6ce5\u3002\u7531\u4e8e\u83b1\u987f\u5f17\u7f57\u65af\u7279\u6548\u5e94\uff08\u6307\u6db2\u4f53\u4e0d\u4f1a\u6da6\u6e7f\u7099\u70ed\u7684\u8868\u9762\uff0c\u800c\u4ec5\u4ec5\u5728\u5176\u4e0a\u5f62\u6210\u4e00\u4e2a\u84b8\u6c7d\u5c42\u7684\u73b0\u8c61\uff09\uff0c\u5f53\u76f4\u63a5\u5c06\u6837\u54c1\u653e\u5165\u6db2\u6c2e\u4e2d\u65f6\uff0c\u6837\u54c1\u5468\u56f4\u7684\u6db2\u6c2e\u4f1a\u7acb\u5373\u6cb8\u817e\uff0c\u5c06\u6837\u54c1\u5305\u88f9\u5728\u7edd\u7f18\u7684\u6c2e\u6c14\u4e2d\uff0c\u4ece\u800c\u4f7f\u6837\u54c1\u4e0d\u80fd\u7acb\u5373\u964d\u6e29\uff0c\u6c34\u6210\u4e3a\u7ed3\u6676\u6001\u3002\u800c\u5c06\u6837\u54c1\u7f6e\u4e8e\u6db2\u6c2e\u6ce5\u4e2d\uff0c\u7531\u4e8e\u6db2\u6c2e\u6ce5\u4e0d\u5b58\u5728\u6cb8\u817e\u73b0\u8c61\uff0c\u6240\u4ee5\u6837\u54c1\u4e0d\u4f1a\u6536\u5230\u83b1\u987f\u5f17\u7f57\u65af\u7279\u6548\u5e94\u7684\u5f71\u54cd\uff0c\u53ef\u8fc5\u901f\u964d\u6e29\u901a\uff0c \u4f7f\u5f97\u6c34\u5206\u5b50\u6765\u4e0d\u53ca\u91cd\u6392\u6210\u6676\u4f53\uff0c\u4ece\u800c\u5f62\u6210\u73bb\u7483\u6001\u7684\u6c34\u3002 \u5982\u679c\u662f\u6db2\u4f53\u6837\u54c1\uff0c\u53ef\u7528\u5939\u7247\u88c5\u8f7d\u6837\u54c1\u76f4\u63a5\u6295\u5165\u5230\u6db2\u6c2e\u6ce5\u4e2d\u8fdb\u884c\u51b7\u51bb\u56fa\u5b9a\uff0c\u56fa\u4f53\u6837\u54c1\u5219\u53ef\u76f4\u63a5\u7528\u4f4e\u6e29\u80f6\u7c98\u5728\u6837\u54c1\u53f0\u4e0a\u6295\u5165\u6db2\u6c2e\u6ce5\u4e2d\u8fdb\u884c\u56fa\u5b9a\u3002<\/p>\n<p><strong> 2.2 \u51b7\u51bb\u65ad\u88c2\/\u55b7\u6d82<\/strong><\/p>\n<p>\u5c06\u5e94\u7528\u9ad8\u538b\u51b7\u51bb\u6216\u6db2\u6c2e\u6ce5\u51b7\u51bb\u56fa\u5b9a\u7684\u6837\u54c1\u5728\u6db2\u6c2e\u4e2d\u8f6c\u79fb\u81f3\u51b7\u51bb\u6837\u54c1\u53f0\u88c5\u8f7d\u88c5\u7f6e\u4e2d\uff0c\u5728\u5176\u4e2d\u5c06\u51b7\u51bb\u597d\u7684\u6837\u54c1\u88c5\u8f7d\u4e8e\u51b7\u51bb\u4f20\u8f93\u6837\u54c1\u6746\u4e0a\u3002\u7136\u540e\u901a\u8fc7\u51b7\u51bb\u4f20\u8f93\u6837\u54c1\u6746\u5c06\u8f7d\u6709\u6837\u54c1\u7684\u6837\u54c1\u53f0\u8f6c\u79fb\u81f3 EM ACE600 \u6e85\u5c04\u4eea\u4e2d\uff08\u56fe 4\uff09\u5e94\u7528\u6e85\u5c04\u4eea\u4e2d\u7684\u5200\u5bf9\u6837\u54c1\u8fdb\u884c\u51b7\u51bb\u65ad\u88c2\uff0c\u9732\u51fa\u6837\u54c1\u65b0\u9c9c\u7684\u65ad\u9762\uff0c\u5bf9\u4e8e\u6709\u4e9b\u6837\u54c1\uff0c\u5728 173K \u4e0b\u8fdb\u884c\u5347\u534e\uff0c\u907f\u514d\u6837\u54c1\u88ab\u6c34\u5305\u88f9\u8986\u76d6\uff0c\u4e4b\u540e\u8fdb\u884c\u5bfc\u7535\u6027\u55b7\u6d82\uff0c\u907f\u514d\u5728\u626b\u63cf\u7535\u955c\u89c2\u5bdf\u8fc7\u7a0b\u4e2d\u653e\u7535\u73b0\u8c61\u7684\u53d1\u751f(\u56fe 5\uff09\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-78191 size-full\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/5-8.png\" alt=\"\" width=\"966\" height=\"452\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/5-8.png 966w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/5-8-300x140.png 300w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/5-8-768x359.png 768w\" sizes=\"(max-width: 966px) 100vw, 966px\" \/><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-78192 size-full\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/6-6.png\" alt=\"\" width=\"965\" height=\"568\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/6-6.png 965w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/6-6-300x177.png 300w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/6-6-768x452.png 768w\" sizes=\"(max-width: 965px) 100vw, 965px\" \/><\/p>\n<p><strong> 2.3 \u51b7\u51bb\u626b\u63cf\u7535\u955c\u89c2\u5bdf<\/strong><\/p>\n<p>\u901a\u8fc7\u51b7\u51bb\u4f20\u8f93\u6837\u54c1\u6746\u5c06\u65ad\u88c2\u3001\u55b7\u6d82\u597d\u7684\u6837\u54c1\u8f6c\u79fb\u81f3\u626b\u63cf\u7535\u955c\u7684\u51b7\u53f0\uff0c\u51b7\u53f0\u662f\u7531\u6db2\u6c2e\u964d\u6e29\u7ecf\u94dc\u5e26\u5bfc\u6e29\u6765\u63a7\u5236\u6e29\u5ea6\uff08 123K\uff09\uff0c\u5728\u626b\u63cf\u7535\u955c\u89c2\u5bdf\u65f6\uff0c\u901a\u5e38\u5e94\u7528\u8f83\u4f4e\u7684\u52a0\u901f\u7535\u538b\u548c\u8f83\u5c0f\u7684\u675f\u6d41\uff0c\u4ee5\u51cf\u5f31\u653e\u7535\u73b0\u8c61\u548c\u7535\u5b50\u675f\u5bf9\u6837\u54c1\u7684\u635f\u4f24\u3002\u7531\u4e8e\u6574\u4e2a\u4f20\u8f93\u53ca\u89c2\u5bdf\u8fc7\u7a0b\u90fd\u4fdd\u6301\u5728\u4f4e\u6e29\u72b6\u6001\uff0c\u6240\u4ee5\u5c3d\u53ef\u80fd\u7684\u4fdd\u6301\u4e86\u6837\u54c1\u5728\u6db2\u76f8\u7684\u539f\u4f4d\u7ed3\u6784\u3002<\/p>\n<p><strong> 3. \u51b7\u51bb\u626b\u63cf\u7535\u955c\u7684\u5e94\u7528\u5b9e\u4f8b<\/strong><\/p>\n<p><strong> 3.1 \u5fae\u4e73\u6db2<\/strong><\/p>\n<p>\u56fe 6 \u4e3a\u5fae\u4e73\u6db2\u6837\u54c1\u5206\u522b\u7ecf\u9ad8\u538b\u51b7\u51bb\u548c\u6db2\u6c2e\u6ce5\u51b7\u51bb\u56fa\u5b9a\uff0c\u5e76\u7ecf\u51b7\u51bb\u65ad\u88c2\/\u55b7\u6d82\u540e\uff0c\u4f20\u8f93\u5230\u51b7\u51bb\u626b\u63cf\u7535\u955c\u5f97\u5230\u7684\u5f62\u8c8c\u56fe\uff0c\u53ef\u4ee5\u770b\u51fa\u51b7\u51bb\u65b9\u6cd5\u80fd\u591f\u4fdd\u6301\u4e73\u6db2\u4e2d\u6db2\u6ef4\u7684\u7ed3\u6784\uff0c\u6db2\u6ef4\u5927\u5c0f\u4e0d\u4e00\uff0c 2 \u00b5m \u81f3 20 \u00b5m\u3002\u4f46\u662f\u5e94\u7528\u9ad8\u538b\u51b7\u51bb\u65b9\u6cd5\u5236\u5f97\u7684\u6837\u54c1\uff0c\u6db2\u6ef4\u5448\u692d\u7403\u578b\uff0c\u53ef\u80fd\u662f\u7531\u4e8e\u6db2\u6ef4\u53d7\u9ad8\u538b\u6324\u538b\u800c\u53d8\u5f62\u6240\u81f4\uff0c\u800c\u5e94\u7528\u6db2\u6c2e\u6ce5\u5236\u6837\u5f97\u5230\u7684\u6837\u54c1\u6db2\u6ef4\u5448\u7403\u578b\uff0c\u4fdd\u6301\u4e86\u5176\u672c\u6765\u7684\u5f62\u8c8c\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-78193 size-full\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/7-4.png\" alt=\"\" width=\"976\" height=\"421\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/7-4.png 976w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/7-4-300x129.png 300w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/7-4-768x331.png 768w\" sizes=\"(max-width: 976px) 100vw, 976px\" \/><\/p>\n<p><strong> 3.2 \u7eb3\u7c73\u7247\u7a33\u5b9a\u7684\u6c34\u5305\u6cb9\u4f53\u7cfb<\/strong><\/p>\n<p>\u56fe 7 \u4e3a\u7528\u7eb3\u7c73\u7247\u7a33\u5b9a\u7684\u5fae\u4e73\u6db2\u7ecf\u6db2\u6c2e\u6ce5\u51b7\u51bb\u56fa\u5b9a\u3001\u5e76\u7ecf\u51b7\u51bb\u65ad\u88c2\/\u55b7\u6d82\u540e\uff0c\u4f20\u8f93\u5230\u51b7\u51bb\u626b\u63cf\u7535\u955c\u5f97\u5230\u7684\u5f62\u8c8c\u56fe\uff0c\u53ef\u4ee5\u770b\u51fa\u7eb3\u7c73\u7247\u5305\u8986\u5728\u6cb9\u6ef4\u8868\u9762\uff0c\u5e76\u4e14\u6cbf\u4e00\u5b9a\u7684\u65b9\u5411\u6392\u5217\uff0c\u636e\u6b64\u53ef\u4ee5\u5f97\u51fa\u7eb3\u7c73\u7247\u5728\u6c34\u5305\u6cb9\u7a33\u5b9a\u4f53\u7cfb\u4e2d\u7684\u5b58\u5728\u72b6\u6001\uff0c\u4ece\u800c\u63a8\u65ad\u7a33\u5b9a\u673a\u7406\u3002<\/p>\n<p>\u56fe 7 \u4e3a\u7528\u7eb3\u7c73\u7247\u7a33\u5b9a\u7684\u5fae\u4e73\u6db2\u7ecf\u6db2\u6c2e\u6ce5\u51b7\u51bb\u56fa\u5b9a\u3001\u5e76\u7ecf\u51b7\u51bb\u65ad\u88c2\/\u55b7\u6d82\u540e\uff0c\u4f20\u8f93\u5230\u51b7\u51bb\u626b\u63cf\u7535\u955c\u5f97\u5230\u7684\u5f62\u8c8c\u56fe\uff0c\u53ef\u4ee5\u770b\u51fa\u7eb3\u7c73\u7247\u5305\u8986\u5728\u6cb9\u6ef4\u8868\u9762\uff0c\u5e76\u4e14\u6cbf\u4e00\u5b9a\u7684\u65b9\u5411\u6392\u5217\uff0c\u636e\u6b64\u53ef\u4ee5\u5f97\u51fa\u7eb3\u7c73\u7247\u5728\u6c34\u5305\u6cb9\u7a33\u5b9a\u4f53\u7cfb\u4e2d\u7684\u5b58\u5728\u72b6\u6001\uff0c\u4ece\u800c\u63a8\u65ad\u7a33\u5b9a\u673a\u7406\u3002<\/p>\n<p><strong> 3.3 \u78f7\u8102\u3001\u4e8c\u80bd\u5206\u5b50\u81ea\u7ec4\u88c5\u4f53<\/strong><\/p>\n<p>\u56fe 8 \u662f\u78f7\u8102\u3001\u4e8c\u80bd\u5206\u5b50\u81ea\u7ec4\u88c5\u4f53\u7ecf\u6db2\u6c2e\u6ce5\u51b7\u51bb\u56fa\u5b9a\u5e76\u7ecf\u51b7\u51bb\u65ad\u88c2\/\u55b7\u6d82\u540e\uff0c\u4f20\u8f93\u5230\u51b7\u51bb\u626b\u63cf\u7535\u955c\u5f97\u5230\u7684\u5f62\u8c8c\u56fe\uff0c\u53ef\u4ee5\u770b\u51fa\u5206\u5b50\u7ec4\u88c5\u6210\u68d2\u72b6\u7ed3\u6784\uff0c\u68d2\u7684\u7c97\u7ec6\u5728 500nm\uff0c\u6bcf\u6839\u68d2\u53c8\u662f\u7531\u591a\u6839\u7ea4\u7ef4\u7ec4\u6210\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-78194 size-full\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/9-3.png\" alt=\"\" width=\"943\" height=\"416\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/9-3.png 943w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/9-3-300x132.png 300w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/9-3-768x339.png 768w\" sizes=\"(max-width: 943px) 100vw, 943px\" \/><\/p>\n<p><strong> 3.4 \u6c34\u51dd\u80f6<\/strong><\/p>\n<p>\u56fe 9 \u662f\u6c34\u51dd\u80f6\u5206\u522b\u7ecf\u8fc7\u51b7\u51bb\u5e72\u71e5\u4e4b\u540e\u5728\u5e38\u6e29\u626b\u63cf\u7535\u955c\u4e0a\u89c2\u5bdf\u5230\u7684\u5f62\u8c8c\u56fe\u548c\u7ecf\u6db2\u6c2e\u6ce5\u51b7\u51bb\u56fa\u5b9a\u5e76\u7ecf\u51b7\u51bb\u65ad\u88c2\/\u55b7\u6d82\u540e\uff0c\u4f20\u8f93\u5230\u51b7\u51bb\u626b\u63cf\u7535\u955c\u5f97\u5230\u7684\u5f62\u8c8c\u56fe\u3002\u53ef\u4ee5\u770b\u51fa\u51b7\u51bb\u5e72\u71e5\u7684\u6c34\u51dd\u80f6\u5b54\u5f84\u8f83\u5927\u4e14\u7a00\u758f\uff0c\u53ef\u80fd\u662f\u7531\u4e8e\u6c34\u5206\u5728\u771f\u7a7a\u4e2d\u6325\u53d1\u540e\u6837\u54c1\u56e0\u4e3a\u6ca1\u6709\u6c34\u5206\u7684\u586b\u5145\u5bfc\u81f4\u5b54\u584c\u9677\u3002\u800c\u672a\u7ecf\u8fc7\u53ef\u4ee5\u770b\u51fa\u5b54\u5f84\u5f88\u5c0f\u4e14\u5bc6\u96c6\uff0c\u56e0\u4e3a\u771f\u7a7a\u5e72\u71e5\u7684\u6c34\u51dd\u80f6\u5728\u51b7\u51bb\u56fa\u5b9a\u7684\u65f6\u5019\u6709\u6c34\u5206\u586b\u5145\uff0c\u4e0d\u4f1a\u5bfc\u81f4\u6837\u54c1\u584c\u9677\uff0c\u56e0\u6b64\u51b7\u51bb\u626b\u63cf\u7535\u955c\u7684\u65b9\u6cd5\u4fdd\u6301\u4e86\u6c34\u51dd\u80f6\u672c\u8eab\u7684\u7ed3\u6784\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-78195 size-full\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/10-1.png\" alt=\"\" width=\"1145\" height=\"457\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/10-1.png 1145w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/10-1-300x120.png 300w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/10-1-768x307.png 768w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/10-1-1024x409.png 1024w\" sizes=\"(max-width: 1145px) 100vw, 1145px\" \/><\/p>\n<p><strong> \u53c2\u8003\u6587\u732e<\/strong><\/p>\n<p>[1] Dimitri, Vanhecke.; Werner, Graber.; Daniel Studer. <i>Methods in cell biology<\/i>. <b>2008<\/b>,<br \/>\n <i>88<\/i>.151<\/p>\n<p>[2] Pederson, R. C.; Miller, C. D.; Arvidson, J. M.; Blount, K.; Schulze, M. <i>Advances\u00a0<\/i><i>in Cryogenic Engineering Materials<\/i>. New York: Springer US, 1998.<\/p>\n<p>[3] Kanno,H.; Speedy, R.J. and Angell, C.A. <i>Science<\/i>,<b>1975<\/b>,<i>189<\/i>, 880.<\/p>\n<p><strong> \u81f4\u8c22<\/strong><br \/>\n \u611f\u8c22\u5206\u6790\u6d4b\u8bd5\u4e2d\u5fc3\u4e01\u4e3d\u840d\u8001\u5e08\u5bf9\u7a3f\u4ef6\u7684\u591a\u6b21\u5ba1\u8bfb\u548c\u7f16\u8f91\u52a0\u5de5\uff01<br \/>\n \u611f\u8c22\u5206\u6790\u6d4b\u8bd5\u4e2d\u5fc3\u8881\u9707\u8001\u5e08\u5728\u51b7\u51bb\u626b\u63cf\u7535\u955c\u5b9e\u9a8c\u8fc7\u7a0b\u4e2d\u5bf9\u4e8e\u6392\u9664\u4eea\u5668\u6545\u969c\u7684\u5e2e\u52a9\uff01<\/p>\n<p><strong><a href=\"http:\/\/www.ceshigu.com\/\" target=\"_blank\">\u6d4b\u8bd5\u8c37<\/a><\/strong>\u662f\u6750\u6599\u4eba\u65d7\u4e0b\u4e13\u6ce8\u6750\u6599\u5206\u6790\u6d4b\u8bd5\u5e73\u53f0\uff0c<strong>\u63d0\u4f9b\u5305\u62ec\u529b\u5b66\u3001\u7269\u7406\u3001\u5316\u5b66\u3001\u7ed3\u6784\u5206\u6790\u7b49\u5206\u6790\u6d4b\u8bd5\u53ca\u5176\u76f8\u5173\u6570\u636e\u5206\u6790<\/strong>\uff0c<strong>\u63d0\u4f9b\u8bba\u6587\u6da6\u8272\u3001\u63d2\u56fe\u7ed8\u5236\u3001\u6750\u6599\u8ba1\u7b97\uff0c\u4e13\u5229\u64b0\u5199\u7b49\u670d\u52a1<\/strong>\u3002\u540c\u65f6\uff0c\u6b22\u8fce\u6709\u4e00\u6280\u4e4b\u957f\u7684\u6750\u6599\u4eba\u5165\u9a7b\u6d4b\u8bd5\u8c37\uff0c\u5411\u5e7f\u5927\u4f1a\u5458\u63d0\u4f9b\u670d\u52a1\uff0c\u626b\u7801\u76f4\u8fbe\u6d4b\u8bd5\u8c37\u670d\u52a1\u4e13\u533a\uff1a<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-78228\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/1491013294-1-300x300-1.png\" alt=\"\" width=\"300\" height=\"300\" \/><\/p>\n<p>\u672c\u6587\u8f6c\u8f7d\u81ea\u4e2d\u79d1\u9662\u5316\u5b66\u6240\u5206\u6790\u6d4b\u8bd5\u4e2d\u5fc3\uff0c\u6750\u6599\u725b\u7f16\u8f91\u6574\u7406\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>1. \u51b7\u51bb\u626b\u63cf\u7535\u955c\u6982\u8ff0 \u5e38\u89c4\u626b\u63cf\u7535\u955c\u8981\u6c42\u6240\u89c2\u5bdf\u7684\u6837\u54c1\u65e0\u6c34\uff0c\u800c\u4e00\u4e9b\u6837\u54c1\u5728\u5e72\u71e5\u8fc7\u7a0b\u4e2d\u4f1a\u53d1\u751f\u7ed3\u6784\u53d8\u5316\uff0c\u81f4\u4f7f\u65e0\u6cd5\u89c2\u5bdf\u5176\u771f\u5b9e\u7ed3\u6784\u3002\u51b7\u51bb\u626b\u63cf\u7535\u955c\u6280\u672f\u80fd\u591f\u5bf9\u542b\u6c34(\u6eb6\u5242)\u6837\u54c1\u8fdb&#8230;<\/p>\n","protected":false},"author":30,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"class_list":["post-78186","post","type-post","status-publish","format-standard","hentry","category-ganhuo"],"_links":{"self":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/78186"}],"collection":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/users\/30"}],"replies":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=78186"}],"version-history":[{"count":9,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/78186\/revisions"}],"predecessor-version":[{"id":78315,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/78186\/revisions\/78315"}],"wp:attachment":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=78186"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=78186"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=78186"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}