{"id":77473,"date":"2017-06-11T06:30:10","date_gmt":"2017-06-10T22:30:10","guid":{"rendered":"\/\/m.iemloyee.com\/?p=77473"},"modified":"2017-06-08T17:17:13","modified_gmt":"2017-06-08T09:17:13","slug":"%e5%85%b1%e8%81%9a%e7%84%a6%e6%98%be%e5%be%ae%e9%95%9c%e4%b8%ad%e8%8d%a7%e5%85%89%e5%9b%a2%e7%9a%84%e5%85%b1%e5%ae%9a%e4%bd%8d","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=77473","title":{"rendered":"\u5b66\u672f\u5e72\u8d27\u4e28\u5171\u805a\u7126\u663e\u5fae\u955c\u4e2d\u8367\u5149\u56e2\u7684\u5171\u5b9a\u4f4d"},"content":{"rendered":"<p>\u5728\u591a\u6807\u8367\u5149\u6837\u54c1\u56fe\u50cf\u4e2d\uff0c\u56e0\u4e24\u4e2a\u6216\u591a\u4e2a\u8367\u5149\u56e2\u5728\u663e\u5fae\u7ed3\u6784\u4e2d\u8ddd\u79bb\u5f88\u8fd1\uff0c\u7ecf\u5e38\u4f1a\u6709\u53d1\u5c04\u4fe1\u53f7\u53e0\u52a0\uff0c\u8fd9\u79cd\u6548\u5e94\u5c31\u79f0\u4e3a\u5171\u5b9a\u4f4d\u3002\u76ee\u524d\uff0c\u9ad8\u7279\u5f02\u6027\u5408\u6210\u8367\u5149\u56e2\u548c\u7ecf\u5178\u514d\u75ab\u8367\u5149\u6280\u672f\u7684\u5e94\u7528\u3001\u7cbe\u5bc6\u5149\u5207\u6280\u672f\u7684\u5e94\u7528\u3001\u5171\u805a\u7126\u548c\u591a\u5149\u5b50\u663e\u5fae\u955c\u63d0\u4f9b\u7684\u6570\u5b57\u56fe\u50cf\u5904\u7406\u6280\u672f\u7b49\u5927\u5927\u63d0\u9ad8\u4e86\u751f\u7269\u6837\u54c1\u4e2d\u5171\u5b9a\u4f4d\u68c0\u6d4b\u7684\u80fd\u529b\u3002<\/p>\n<p><a href=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/06\/1-7.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-85837\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/06\/1-7.png\" alt=\"\" width=\"650\" height=\"236\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/06\/1-7.png 650w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/06\/1-7-300x109.png 300w\" sizes=\"(max-width: 650px) 100vw, 650px\" \/><\/a><\/p>\n<p style=\"text-align: center;\"><strong> \u56fe 1<\/strong><\/p>\n<p style=\"text-align: left;\">\u5171\u5b9a\u4f4d\u5728\u751f\u7269\u8868\u8ff0\u4e0a\u662f\u8fd9\u6837\u5b9a\u4e49\u7684\uff1a\u4e24\u4e2a\u6216\u591a\u4e2a\u4e0d\u540c\u7684\u5206\u5b50\u4f4d\u4e8e\u6837\u54c1\u4e0a\u540c\u4e00\u4e2a\u7269\u7406\u4f4d\u7f6e\u3002\u5bf9\u663e\u5fae\u955c\u4e2d\u770b\u5230\u7684\u7ec4\u7ec7\u5207\u7247\u3001\u5355\u4e2a\u7ec6\u80de\u6216\u4e9a\u7ec6\u80de\u5668\u5b98\uff0c\u5171\u5b9a\u4f4d\u610f\u5473\u7740\u4e0d\u540c\u7684\u5206\u5b50\u8fde\u63a5\u5230\u540c\u4e00\u4e2a\u53d7\u4f53\u4e0a\uff1b\u5728\u6570\u5b57\u56fe\u50cf\u65b9\u9762\uff0c\u8fd9\u4e2a\u672f\u8bed\u6307\u7684\u662f\u4e0d\u540c\u8367\u5149\u5206\u5b50\u53d1\u5c04\u7684\u989c\u8272\u5206\u4eab\u56fe\u50cf\u4e2d\u7684\u540c\u4e00\u4e2a\u50cf\u7d20\u3002\u5728\u5171\u805a\u7126\u663e\u5fae\u955c\u4e2d\uff0c\u6837\u54c1\u88ab\u8bb0\u5f55\u6210\u542b\u6709\u5f88\u591a\u50cf\u5143\u7684\u591a\u7ef4\u9635\u5217\u6570\u5b57\u56fe\u50cf\uff0c\u6bcf\u4e2a\u50cf\u5143\u4ee3\u8868\u4e00\u4e2a\u4e09\u7ef4\u50cf\u7d20\u3002\u50cf\u5143\u7684\u5c3a\u5bf8\uff08\u6216\u68c0\u6d4b\u5355\u5143\uff09\u7531\u7269\u955c\u7684\u6570\u503c\u5b54\u5f84\u3001\u7167\u660e\u6ce2\u957f\u4ee5\u53ca\u5171\u7126\u68c0\u6d4b\u9488\u5b54\u76f4\u5f84\u7b49\u51b3\u5b9a\u3002\u56e0\u6b64\uff0c\u5728\u4e00\u4e2a\u6837\u54c1\u4e2d\u4e24\u4e2a\u8367\u5149\u63a2\u9488\u7684\u5171\u5b9a\u4f4d\uff0c\u6bd4\u5982\u53d1\u7eff\u5149\u7684 Alexa Fluor488\uff0c\u53d1\u6a58\u7ea2\u8272\u5149\u7684 Cy3\uff0c\u5728\u56fe\u50cf\u4e2d\u5c31\u662f\u7531\u542b\u6709\u7ea2\u8272\u548c\u7eff\u8272\u4e24\u8005\u8d21\u732e\u7684\u50cf\u7d20\u8868\u793a\uff08\u7ecf\u5e38\u4ea7\u751f\u5404\u79cd\u5404\u6837\u7684\u6a58\u8272\u548c\u9ec4\u8272\uff09\u3002<\/p>\n<p style=\"text-align: left;\">\u4e3e\u4e00\u4e2a\u4f8b\u5b50\uff0c \u56fe 1 \u7684\u4e00\u7cfb\u5217\u56fe\u8868\u660e\u4e86\u9aa8\u9abc\u808c\u52a8\u86cb\u767d\u548c\u9ecf\u7740\u6591\u86cb\u767d\u4fa7\u5411\u5149\u5b66\u5e73\u9762\u4e0a\u7684\u5171\u5b9a\u4f4d\uff08\u6fc0\u5149\u626b\u63cf\u5171\u805a\u7126\u663e\u5fae\u955c\u4e2d\u7684 XY \u9762\uff09\u3002\u8fd9\u4e9b\u5171\u5b9a\u4f4d\u70b9\u53ef\u4f5c\u4e3a\u808c\u52a8\u86cb\u767d\u4e1d\u7684\u6210\u6838\u4f4d\u70b9\uff0c\u4e5f\u53ef\u4f5c\u4e3a\u5916\u90e8\u4ecb\u8d28\u3001\u8d28\u819c\u548c\u808c\u52a8\u86cb\u767d\u9aa8\u67b6\u4e4b\u95f4\u7684\u4ea4\u8054\u5242\u3002\u56fe 1\uff08 a\uff09\u662f Alexa Fluor 568 \u901a\u9053\uff08\u76ee\u6807\u5bf9\u8c61\u662f\u9ecf\u7740\u6591\u86cb\u767d\uff09\uff0c\u7531 543nm \u7684\u6c26\u6c16\u6fc0\u5149\u5668\u6fc0\u53d1\uff1b\u800c Figure1\uff08 b\uff09\u662f Alexa Fluor488 \u901a\u9053(\u76ee\u6807\u5bf9\u8c61\u662f\u4e1d\u72b6\u808c\u52a8\u86cb\u767d)\uff0c\u7531 488nm \u7684\u6c29\u79bb\u5b50\u6fc0\u5149\u5668\u6fc0\u53d1\uff1b Figure1\uff08 c\uff09\u662f\u524d\u9762\u4e24\u5e45\u56fe\u7684\u53e0\u52a0\uff0c\u8868\u660e\u4e24\u79cd\u8367\u5149\u4fe1\u53f7\u5728\u4e1d\u72b6\u808c\u52a8\u86cb\u767d\u7ea4\u7ef4\u672b\u7aef\u7684\u5171\u5b9a\u4f4d\u3002\u5fc5\u987b\u6307\u51fa\u7684\u662f\uff0c\u5171\u5b9a\u4f4d\u5e76\u4e0d\u6307\u7684\u662f\u5177\u6709\u76f8\u4f3c\u53d1\u5c04\u5149\u8c31\u7684\u8367\u5149\u56e2\u51fa\u73b0\u5728\u5408\u6210\u56fe\u50cf\u7684\u540c\u4e00\u4e2a\u50cf\u7d20\u4e0a\u3002\u51c6\u786e\u7684\u5171\u5b9a\u4f4d\u5206\u6790\u53ea\u6709\u5728\u8367\u5149\u56e2\u7684\u8367\u5149\u53d1\u5c04\u5149\u8c31\u8db3\u591f\u5206\u79bb\u4e14\u6ee4\u8272\u7247\uff08\u6216\u5149\u8c31\u72ed\u7f1d\u5bbd\u5ea6\uff09\u6b63\u786e\u8bbe\u7f6e\u7684\u60c5\u51b5\u4e0b\u624d\u6709\u53ef\u80fd\u3002\u8367\u5149\u56e2\u53d1\u5c04\u5149\u8c31\u4e4b\u95f4\u7684\u5927\u91cf\u53e0\u52a0\u6216\u6ee4\u8272\u7247\u7ec4\u5408\u7684\u4e0d\u6b63\u786e\u4f7f\u7528\uff0c\u90fd\u53ef\u80fd\u5bfc\u81f4\u5149\u8c31\u4e32\u8272\uff0c\u8fd9\u79cd\u60c5\u51b5\u4e0b\u5171\u5b9a\u4f4d\u7684\u6d4b\u91cf\u5c31\u6ca1\u6709\u610f\u4e49\u3002\u4e3a\u4e86\u907f\u514d\u4ea7\u751f\u5171\u5b9a\u4f4d\u5047\u8c61\uff0c\u8367\u5149\u56e2\u5fc5\u987b\u4e0e\u7167\u660e\u5149\u6e90\u7684\u5149\u8c31\u8ba4\u771f\u5339\u914d\uff08\u5171\u805a\u7126\u4e2d\u662f\u6fc0\u5149\u7ebf\uff09\uff0c\u6765\u83b7\u5f97\u6700\u5927\u6fc0\u53d1\u6548\u7387\uff0c\u540c\u65f6\u5728\u53d1\u5c04\u5149\u8c31\u4e4b\u95f4\u4fdd\u6301\u4e00\u5b9a\u7684\u5206\u79bb\u5ea6\u3002\u5728\u5927\u591a\u6570\u60c5\u51b5\u4e0b\uff0c\u5bf9\u5171\u5b9a\u4f4d\u5206\u6790\u6765\u8bf4\uff0c\u8367\u5149\u56e2\u7684\u9009\u62e9\u5bf9\u83b7\u5f97\u6ee1\u610f\u7ed3\u679c\u6781\u4e3a\u91cd\u8981\u3002<\/p>\n<p style=\"text-align: left;\">\u5728\u56fe 2 \u4e2d\uff0c\u5bf9 Alexa Fluor \u67d3\u6599\u5bb6\u65cf\u7684\u5149\u8c31\u53e0\u52a0\u4f5c\u4e86\u6bd4\u8f83\uff0c\u8fd9\u5728\u5171\u5b9a\u4f4d\u5b9e\u9a8c\u4e2d\u662f\u5f88\u6709\u7528\u7684\u3002\u4e3a\u4e86\u6bd4\u8f83\uff0c\u6240\u6709\u7684\u53d1\u5c04\u5149\u8c31\u90fd\u505a\u4e86\u5f52\u4e00\u5316\uff0c\u53e0\u52a0\u533a\u57df\u7528\u7070\u8272\u9634\u5f71\u663e\u793a\u3002\u5728\u56fe 2(a) \u4e2d\uff0c\u7eff\u8272\u8367\u5149\u67d3\u6599 Alexa Fluor 488 \u548c\u6a59\u9ec4\u8272\u8367\u5149\u67d3\u6599Alexa Fluor 555 \u5728\u5cf0\u6ce2\u957f\u5904\u663e\u793a\u5f88\u660e\u663e\u7684\u5206\u79bb\uff0c\u4eba\u773c\u4e5f\u5f88\u5bb9\u6613\u80fd\u591f\u533a\u5206\u3002\u7136\u800c\uff0c\u5149\u8c31\u53e0\u52a0\uff08\u7070\u8272\u9634\u5f71\u533a\u57df\uff09\u8868\u660e\u5728 Alexa Fluor 555 \u7684\u53d1\u5c04\u5cf0\u4e0a\u5f88\u660e\u663e\u6709 Alexa\u00a0Fluor488 \u7684\u53d1\u5c04\u5149\u8c31\uff08\u7528\u4e00\u6761\u9ed1\u7ebf\u6807\u793a\uff0c\u4ece\u53d1\u5c04\u5cf0\u5230\u6a2a\u5750\u6807\uff09\u3002\u5f53 Alexa Fluor\u00a0488 \u7684\u8367\u5149\u53d1\u5c04\u5f3a\u5ea6\u660e\u663e\u6bd4 Alexa Fluor 555 \u7684\u8367\u5149\u53d1\u5c04\u5f3a\u65f6\uff0c\u8367\u5149\u4fe1\u53f7\u8fd9\u4e48\u9ad8\u7a0b\u5ea6\u7684\u4e32\u8272\u4f7f\u5f97\u8367\u5149\u63a2\u9488\u7684\u5206\u79bb\u5f88\u96be\u3002\u5f88\u591a\u56e0\u7d20\u90fd\u4f1a\u5bfc\u81f4\u8fd9\u79cd\u60c5\u51b5\u53d1\u751f\uff0c\u6bd4\u5982\u8367\u5149\u56e2\u6807\u7684\u7269\u6d53\u5ea6\u7684\u5de8\u5927\u5dee\u5f02\u3002\u56e0\u6b64\uff0c\u5728\u5171\u5b9a\u4f4d\u5b9e\u9a8c\u4e2d\uff0c\u8fd9\u4e9b\u63a2\u9488\u7684\u7ec4\u5408\u5c31\u5e94\u8be5\u907f\u514d\uff0c\u6216\u53ea\u6709\u5f53\u56fe\u50cf\u5728\u591a\u901a\u9053\u5171\u7126\u6a21\u5f0f\u91c7\u96c6\u4e0b\u624d\u53ef\u4ee5\u4f7f\u7528\uff0c\u8fd9\u6837\u53ef\u4ee5\u964d\u4f4e\u6216\u6d88\u9664\u4e32\u8272\u3002<\/p>\n<p style=\"text-align: left;\"><a href=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/06\/\u56fe2.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-85838\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/06\/\u56fe2.png\" alt=\"\" width=\"650\" height=\"317\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/06\/\u56fe2.png 650w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/06\/\u56fe2-300x146.png 300w\" sizes=\"(max-width: 650px) 100vw, 650px\" \/><\/a><\/p>\n<p style=\"text-align: center;\"><strong> \u56fe 2<\/strong><\/p>\n<p style=\"text-align: left;\">Alexa Fluor \u63a2\u9488\u4e4b\u95f4\u7684\u5149\u8c31\u53e0\u52a0\u7a0b\u5ea6\u4f1a\u968f\u7740\u63a2\u9488\u53d1\u5c04\u5cf0\u4e4b\u95f4\u7684\u8ddd\u79bb\u589e\u52a0\u800c\u4e0b\u964d\uff0c\u5982 Figure2(b)\u6240\u793a\u3002\u5728\u8fd9\u79cd\u60c5\u51b5\u4e0b\uff0c Alexa Fluor 488 \u548c\u6df1\u7ea2\u8272\u67d3\u6599 Alexa\u00a0Fluor 633 \u4e0e Figure2 (a)\u6bd4\u8f83\uff0c\u91cd\u53e0\u533a\u57df\u660e\u663e\u964d\u4f4e\u3002\u8fd9\u4e24\u79cd\u67d3\u6599\u4eba\u773c\u90fd\u5f88\u5bb9\u6613\u533a\u5206\uff0c\u5149\u8c31\u91cd\u53e0\u7a0b\u5ea6\u4f4e\u5728\u5171\u5b9a\u4f4d\u5b9e\u9a8c\u4e2d\u53ef\u4f7f\u4e32\u8272\u6700\u5c0f\u5316\uff0c\u5982\u6bcf\u4e2a\u63a2\u9488\u7684\u6d53\u5ea6\u76f8\u4f3c\u7684\u8bdd\uff0c\u5e94\u8be5\u53ef\u4ee5\u4ea7\u751f\u8f83\u597d\u7684\u7ed3\u679c\uff08\u6ce8\u610f\uff1a\u6df1\u7ea2\u8272\u7684\u8367\u5149\u67d3\u6599 Alexa Fluor 633 \u5728\u4f4e\u6d53\u5ea6\u65f6\u901a\u8fc7\u663e\u5fae\u955c\u76ee\u955c\u5f88\u96be\u89c2\u5bdf\u5230\uff09\u3002 Alexa Fluor 633 \u53ef\u88ab\u7ea2\u8272\u6c26\u6c16\u6fc0\u5149\u5668\u7684 633nm \u7ebf\u6700\u6709\u6548\u7684\u6fc0\u53d1\uff0c\u4e5f\u53ef\u88ab\u9ec4\u8272\u6c26\u6c16\u6fc0\u5149\u5668\u7684 594nm \u6fc0\u53d1\u3002\u6216\u8bb8\uff0c\u5728 Alexa\u00a0Fluor\u67d3\u6599\u53d1\u5c04\u7684\u53ef\u89c1\u5149\u533a\u57df\uff0c\u6700\u597d\u7684\u5149\u8c31\u5206\u79bb\u662f Alexa Fluor 488\u548c Alexa Fluor\u00a0647 \u7684\u7ed3\u5408\uff08\u56fe\u4e2d\u672a\u663e\u793a\uff09\u3002\u5b9e\u9645\u4e0a\uff0c\u5728\u8fd9\u4e9b\u67d3\u6599\u4e4b\u95f4\u6ca1\u6709\u5149\u8c31\u91cd\u53e0\uff0c\u5373\u4f7f\u6837\u54c1\u542b\u6709\u8fc7\u91cf\u7684 Alexa Fluor 488\uff0c\u5e94\u8be5\u4e5f\u6ca1\u6709\u4e32\u8272\u3002\u5177\u6709\u8fd9\u4e9b\u7279\u70b9\u7684\u8367\u5149\u63a2\u9488\u662f\u5171\u805a\u7126\u663e\u5fae\u955c\u5206\u6790\u5171\u5b9a\u4f4d\u7684\u7406\u60f3\u9009\u62e9\u3002<\/p>\n<p style=\"text-align: left;\">\u5728\u5171\u805a\u7126\u663e\u5fae\u955c\u4e2d\uff0c\u6d4b\u5b9a\u5171\u5b9a\u4f4d\u7684\u80fd\u529b\u53d7\u9650\u4e8e\u5149\u5b66\u7cfb\u7edf\u7684\u5206\u8fa8\u7387\u53ca\u7528\u4e8e\u7167\u660e\u6837\u54c1\u7684\u5165\u5c04\u5149\u6ce2\u957f\u3002\u5bbd\u573a\u8367\u5149\u548c\u5171\u805a\u7126\u663e\u5fae\u955c\u7406\u8bba\u5206\u8fa8\u7387\u7ea6\u4e3a 200nm\uff0c\u4f46\u5728\u5b9e\u9645\u4e0a\uff0c\u7531\u4e8e\u5404\u79cd\u539f\u56e0\u8fd9\u4e2a\u6570\u964d\u5230 400nm \u548c 600nm \u4e4b\u95f4\uff0c\u539f\u56e0\u5305\u62ec\u663e\u5fae\u955c\u5149\u8def\u672a\u5b8c\u5168\u5bf9\u51c6\u3001\u5149\u5b66\u6298\u5c04\u7387\u6ce2\u52a8\u3001\u5149\u5b66\u50cf\u5dee\u53ca\u6837\u54c1\u5236\u5907\u7684\u4e0d\u5408\u9002\u3002\u7136\u800c\uff0c\u5bf9\u4e8e\u4e00\u4e2a\u5df2\u7ecf\u5b8c\u5168\u8c03\u597d\u7684\u5171\u805a\u7126\u663e\u5fae\u955c\u6765\u8bf4\uff0c\u4e24\u4e2a\u8367\u5149\u5206\u5b50\u662f\u5426\u8fde\u63a5\u5230\u540c\u4e00\u4e2a\u76ee\u6807\u5bf9\u8c61\u4e0a\uff0c\u6216\u8005\u5b83\u4eec\u662f\u5426\u5b9a\u4f4d\u5728\u540c\u4e00\u4e2a\u5668\u5b98\u4e0a\u53d7\u5149\u5b66\u5206\u8fa8\u7387\u5f71\u54cd\u3002\u5171\u5b9a\u4f4d\u7684\u8bb8\u591a\u5b9e\u9a8c\u662f\u56f4\u7ed5\u9ad8\u7279\u5f02\u6027\u7684\u5408\u6210\u63a2\u9488\u548c\u6297\u4f53\u8fdb\u884c\u7684\uff0c\u6807\u8bb0\u76ee\u6807\u662f\u5bb9\u6613\u533a\u5206\u7684\u5c40\u90e8\u7684\u3001\u660e\u786e\u7684\u7ec6\u80de\u7ed3\u6784\u3002<\/p>\n<p style=\"text-align: left;\">\u5bf9\u4e8e\u539a\u5ea6\u5c0f\u4e8e 5\u03bcm \u7684\u6837\u54c1\uff0c\u6bd4\u5982\u8d34\u58c1\u7ec6\u80de\u6216\u5f88\u8584\u7684\u7ec4\u7ec7\u5207\u7247\uff0c\u5728\u5e38\u89c4\u7684\u5bbd\u573a\u8367\u5149\u663e\u5fae\u955c\u4e0b\uff0c\u5b9a\u91cf\u7684\u5171\u5b9a\u4f4d\u5206\u6790\u4e00\u822c\u662f\u53ef\u4ee5\u7684\u3002\u7136\u800c\uff0c\u5bf9\u4e8e\u539a\u6837\u54c1\uff0c\u56fe\u50cf\u5e94\u4ee5\u5177\u6709\u4e00\u5b9a\u8f74\u5411\u5c3a\u5bf8\u7684\u5149\u5b66\u5207\u7247\u6765\u8bb0\u5f55\uff0c\u6765\u5206\u6790\u770b\u8d77\u6765\u5171\u5b9a\u4f4d\u7684\u8367\u5149\u56e2\u662f\u5426\u771f\u6b63\u4f4d\u4e8e\u540c\u4e00\u4e2a\u4fa7\u5411\u7126\u5e73\u9762\u4e0a\uff0c\u6216\u5728 Z 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left;\">\u6837\u54c1\u4e2d\u8367\u5149\u56e2\u5171\u5b9a\u4f4d\u7684\u7a0b\u5ea6\u662f\u901a\u8fc7\u6bd4\u8f83\u4e00\u5e45\u56fe\u4e0a\u6bcf\u4e2a\u50cf\u7d20\u4f4d\u7f6e\u7684\u989c\u8272\u503c\u6d4b\u5b9a\u7684\u3002\u5206\u6790\u7684\u7b2c\u4e00\u6b65\u5c31\u662f\u8981\u663e\u793a\u8fdb\u884c\u5171\u5b9a\u4f4d\u6d4b\u91cf\u7684\u56fe\uff0c\u4e00\u822c\u4ee5\u4e24\u4e2a\u72ec\u7acb\u901a\u9053\u7684\u5408\u6210\u56fe\u663e\u793a\u3002\u5f53\u5206\u6790\u591a\u6807\u6837\u54c1\u65f6\uff0c\u5728\u4e00\u6b21\u8ba1\u7b97\u4e2d\uff0c\u53ea\u80fd\u5904\u7406\u4e24\u79cd\u4f2a\u5f69\uff0c\u4f46\u6240\u6709\u4f2a\u5f69\u6392\u5217\u4e4b\u540e\u90fd\u53ef\u4ee5\u914d\u5bf9\u7528\u4e8e\u5171\u5b9a\u4f4d\u5206\u6790\u3002\u7531\u4e8e\u4f20\u7edf\u4e0a\u4f7f\u7528\u6c29\u79bb\u5b50\u6fc0\u5149\u5668\u3001\u6c2a-\u6c29\u79bb\u5b50\u6fc0\u5149\u5668\u53ca\u6c26\u6c16\u6fc0\u5149\u5668\uff0c\u8fd9\u4e9b\u6fc0\u5149\u5668\u80fd\u591f\u6709\u6548\u5730\u6fc0\u53d1\u5728\u84dd\u8272\u548c\u7eff\u8272\u533a\u57df\u6709\u5f3a\u70c8\u5438\u6536\u7684\u8367\u5149\u56e2\uff0c\u56e0\u6b64\u9009\u62e9\u7ea2\u7eff\u989c\u8272\u5bf9\u4f5c\u4e3a\u5171\u805a\u7126\u8367\u5149\u989c\u8272\u3002\u6b64\u5916\uff0c\u4eba\u773c\u5bf9\u7eff\u8272\u548c\u7ea2\u8272\u8272\u8c03\u66f4\u4e3a\u654f\u611f\u3002<\/p>\n<p style=\"text-align: left;\">\u56fe\u50cf\u7684\u5171\u5b9a\u4f4d\u5206\u6790\u4e00\u822c\u7ecf\u5e38\u7528\u6563\u70b9\u56fe\u8868\u793a\uff08 scatterplot\uff09\uff0c\u8fd9\u4e2a\u56fe\u5c06\u4e24\u5957\u6570\u636e\u5173\u8054\u8d77\u6765\u3002\u6563\u70b9\u56fe\u4ee5\u4e8c\u7ef4\u56fe\u7684\u5f62\u5f0f\u63cf\u8ff0\u4e86\u4e00\u5e45\u56fe\u6216\u4e00\u4e2a\u611f\u5174\u8da3\u533a\u57df\u6bcf\u4e2a\u50cf\u7d20\u5904\u4e00\u4e2a\u901a\u9053\u5bf9\u53e6\u4e00\u4e2a\u901a\u9053\u7684\u5f3a\u5ea6\u503c\uff08\u89c1\u56fe 3 \u548c\u56fe 4\uff09\u3002\u4f5c\u56fe\u65f6\u5176\u4e2d\u4e00\u4e2a\u901a\u9053\uff08\u901a\u5e38\u662f\u7eff\u8272\uff09\u4f5c\u4e3a x \u8f74\uff0c\u800c\u53e6\u4e00\u4e2a\u901a\u9053\uff08\u901a\u5e38\u662f\u7ea2\u8272\uff09\u4f5c\u56fe\u65f6\u4f5c\u4e3a y \u8f74\uff0c\u5728\u6a2a\u5750\u3002\u6807\u548c\u7eb5\u5750\u6807\u4e0a\u5f3a\u5ea6\u8303\u56f4\u662f 0\u223c4095. \u56e0\u6b64\uff0c\u5408\u6210\u56fe\u7684\u6bcf\u4e2a\u50cf\u7d20\u70b9\u90fd\u6709\u4e00\u5bf9\u5f3a\u5ea6\u503c\u3002\u5206\u6790\u6bcf\u4e00\u5bf9\u5f3a\u5ea6\u503c\u4ea7\u751f\u7684\u5206\u5e03\u56fe\u6848\uff0c\u80fd\u591f\u8bc6\u522b\u8367\u5149\u56e2\u7684\u5171\u5b9a\u4f4d\u3001\u533a\u5206\u80cc\u666f\u3001\u4e32\u8272\u3001\u5149\u6f02\u767d\u7b49\u3002<\/p>\n<p style=\"text-align: left;\"><a href=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/06\/\u56fe3.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-85839\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/06\/\u56fe3.png\" alt=\"\" width=\"650\" height=\"504\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/06\/\u56fe3.png 650w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/06\/\u56fe3-300x233.png 300w\" sizes=\"(max-width: 650px) 100vw, 650px\" \/><\/a><\/p>\n<p style=\"text-align: center;\"><strong> \u56fe 3<\/strong><\/p>\n<p style=\"text-align: left;\">\u56fe 3 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\u7684\u6563\u70b9\u56fe\uff0c\u4e24\u4e2a\u901a\u9053\u5171\u5b9a\u4f4d\u7684\u7a0b\u5ea6\u5728\u6bcf\u4e2a\u6563\u70b9\u56fe\u7684\u5de6\u4e0b\u89d2\u7528\u767d\u8272\u5b57\u6bcd\u548c\u6570\u5b57\u8868\u793a\u3002\u56fe 3a \u5171\u5b9a\u4f4d\u7a0b\u5ea6\u76f8\u5bf9\u8f83\u5c0f\uff08\u53ea\u6709\u767e\u5206\u4e4b\u51e0\uff09\uff0c\u5728\u56fe 3b \u548c\u56fe 3c \u4e2d\u5171\u5b9a\u4f4d\u7a0b\u5ea6\u9010\u6e10\u589e\u52a0\uff0c\u5171\u5b9a\u4f4d\u7cfb\u6570\u5206\u522b\u662f 30%\u548c85%\u3002<\/p>\n<p style=\"text-align: left;\">\u5171\u805a\u7126\u663e\u5fae\u955c\u53ca\u914d\u4ef6\u5236\u9020\u5546\u63d0\u4f9b\u7684\u8f6f\u4ef6\u53ef\u5bf9\u8367\u5149\u56e2\u5171\u5b9a\u4f4d\u8fdb\u884c\u6563\u70b9\u56fe\u5206\u6790\u3002\u56fe 4 \u663e\u793a\u4e86\u4e00\u7cfb\u5217\u5206\u6790\u56fe\uff0c\u8fd9\u4e2a\u56fe\u662f\u5370\u5ea6\u9e82\u9e7f\u76ae\u80a4\u6210\u7ea4\u7ef4\u7ec6\u80de\uff0c\u7528 AlexaFluor568 \u67d3\u8272\uff08\u6807\u8bb0\u5bf9\u8c61\u662f\u9ecf\u7740\u6591\u86cb\u767d\uff0c\u7ea2\u8272\u901a\u9053\uff09\uff0c\u540c\u65f6\u7528 Alexa Fluor488\u67d3\u8272\uff08\u6807\u8bb0\u5bf9\u8c61\u662f\u7ea4\u7ef4\u72b6\u808c\u52a8\u86cb\u767d\uff0c\u7eff\u8272\u901a\u9053\uff09\u3002\u5728\u6563\u70b9\u56fe\u4e2d\u9009\u62e9\u4e00\u4e2a\u611f\u5174\u8da3\u533a\u57df\u8fdb\u884c\u5206\u6790\uff0c\u5728\u56fe 4a \u4e2d\u7528\u767d\u989c\u8272\u7684\u957f\u65b9\u5f62\u4e3a\u8fd9\u4e2a\u611f\u5174\u8da3\u533a\u57df\u8bbe\u5b9a\u4e86\u4fe1\u53f7\u7684\u9608\u503c\uff0c\u5927\u591a\u6570\u5171\u5b9a\u4f4d\u5206\u6790\u8f6f\u4ef6\u90fd\u53ef\u4ee5\u8fdb\u884c\u8fd9\u79cd\u529f\u80fd\u5206\u6790\u3002<\/p>\n<p style=\"text-align: left;\">\u611f\u5174\u8da3\u533a\u57df\u7684\u6c34\u5e73\u8fb9\u754c\u548c\u7ad6\u76f4\u8fb9\u754c\u5e94\u8be5\u6392\u9664\u80cc\u666f\u4fe1\u53f7\uff0c\u80cc\u666f\u6cbf\u7740\u6563\u70b9\u56fe\u7684 x\u8f74\u548c y \u8f74\u56e2\u7c07\u3002\u53ea\u6709\u88ab\u5305\u62ec\u5728\u6240\u9009\u62e9\u533a\u57df\u8fb9\u754c\u5185\u7684\u50cf\u7d20\u4fe1\u53f7\u624d\u80fd\u8fdb\u884c\u5171\u5b9a\u4f4d\u5206\u6790\u3002\u6837\u54c1\u4e0a\u91cd\u53e0\u7684\u50cf\u7d20\u533a\u57df\u5f88\u5bb9\u6613\u8f6c\u53d8\u6210\u5171\u5b9a\u4f4d\u4e8c\u5143\u9608\u503c\u50cf(\u56fe 4b)\uff0c\u8fd9\u4e2a\u56fe\u8fd8\u53ef\u4ee5\u548c\u5171\u805a\u7126\u56fe\u53e0\u52a0\u5728\u4e00\u8d77\uff0c\u505a\u4e00\u4e2a\u5171\u5b9a\u4f4d map. \u56fe 4c \u663e\u793a\u7684\u5171\u5b9a\u4f4d map \u56fe\u7528\u767d\u989c\u8272\u663e\u793a\u4e86\u5171\u5b9a\u4f4d\u533a\u57df\uff0c\u5bf9\u5927\u591a\u6570\u8f6f\u4ef6\u8fd9\u4e2a\u989c\u8272\u5f88\u5bb9\u6613\u53d8\u6210\u5176\u4ed6\u989c\u8272\uff0c\u76f8\u5bf9\u4e8e\u539f\u6765\u7684\u4f2a\u5f69\uff0c\u5bf9\u6bd4\u5ea6\u66f4\u9ad8\u4e00\u4e9b\u3002<\/p>\n<p style=\"text-align: left;\"><a href=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/06\/\u56fe4.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-85840\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/06\/\u56fe4.png\" alt=\"\" width=\"650\" height=\"262\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/06\/\u56fe4.png 650w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/06\/\u56fe4-300x121.png 300w\" sizes=\"(max-width: 650px) 100vw, 650px\" \/><\/a><\/p>\n<p style=\"text-align: center;\"><strong> \u56fe 4<\/strong><\/p>\n<p style=\"text-align: left;\">\u6b63\u5982\u4e0a\u9762\u6240\u8ba8\u8bba\u7684\uff0c\u5728\u5171\u805a\u7126\u56fe\u4e2d\u5bf9\u8367\u5149\u56e2\u5171\u5b9a\u4f4d\u7684\u5b9a\u91cf\u6d4b\u5b9a\uff0c\u53ef\u901a\u8fc7\u6563\u70b9\u56fe\u548c\u611f\u5174\u8da3\u533a\u57df\u7684\u4fe1\u606f\u83b7\u5f97\u3002\u4ece\u6574\u4e2a\u6563\u70b9\u56fe\u7684\u4fe1\u606f\uff0c\u53ef\u83b7\u5f97\u5f88\u591a\u53d8\u91cf\u503c\u3002Pearson\u2032s \u7cfb\u6570\u5c31\u662f\u7528\u4e8e\u5206\u6790\u6574\u4e2a\u6563\u70b9\u56fe\u7684\u8bf8\u591a\u53d8\u91cf\u4e2d\u7684\u4e00\u4e2a\uff0c\u4e3a\u63cf\u8ff0\u4e24\u5e45\u56fe\u4e4b\u95f4\u91cd\u53e0\u7a0b\u5ea6\uff0c\u5728\u8bc6\u522b\u4e00\u5e45\u56fe\u50cf\u548c\u53e6\u4e00\u5e45\u56fe\u50cf\u7684\u5339\u914d\u7a0b\u5ea6\u4e0a\uff0c Pearson\u2032s, R(r)\u7cfb\u6570\u662f\u6807\u51c6\u6280\u672f\u4e4b\u4e00\u3002 Pearson\u2032s \u76f8\u5173\u7cfb\u6570\u6839\u636e\u4e0b\u9762\u65b9\u7a0b\u8ba1\u7b97\uff1a<\/p>\n<p style=\"text-align: left;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-77478 size-medium\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/QQ\u622a\u56fe20170424105207-300x82.png\" alt=\"\" width=\"300\" height=\"82\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/QQ\u622a\u56fe20170424105207-300x82.png 300w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/QQ\u622a\u56fe20170424105207.png 644w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/p>\n<p style=\"text-align: left;\"><b>S1 <\/b>\u662f\u7b2c\u4e00\u4e2a\u901a\u9053\u6bcf\u4e2a\u50cf\u7d20\u7684\u5f3a\u5ea6\u503c\uff0c\u800c <b>S2 <\/b>\u662f\u7b2c\u4e8c\u4e2a\u901a\u9053\u6bcf\u4e2a\u50cf\u7d20\u7684\u5f3a\u5ea6\u503c\u3002<b>S1(average)<\/b>\u548c<b>S2(average)<\/b>\u5206\u522b\u662f\u7b2c\u4e00\u4e2a\u901a\u9053\u548c\u7b2c\u4e8c\u4e2a\u901a\u9053\u5e73\u5747\u50cf\u7d20\u5f3a\u5ea6\u503c\u3002\u5728 Pearson\u2032s \u7cfb\u6570\u91cc\uff0c\u539f\u59cb\u50cf\u7d20\u5f3a\u5ea6\u503c\u51cf\u53bb\u5e73\u5747\u50cf\u7d20\u5f3a\u5ea6\u503c\u3002\u7ed3\u679c\uff0c\u7cfb\u6570\u503c\u8303\u56f4\u4ece-1\u5230 1\uff0c -1 \u8868\u793a\u56fe\u50cf\u7684\u50cf\u7d20\u4e4b\u95f4\u5b8c\u5168\u6ca1\u6709\u91cd\u53e0\uff0c\u800c 1 \u8868\u793a\u5b8c\u7f8e\u7684\u56fe\u50cf\u91cd\u53e0\u3002Pearson\u2032s\u76f8\u5173\u7cfb\u6570\u53ea\u89e3\u91ca\u4e86\u4e24\u4e2a\u56fe\u50cf\u4e4b\u95f4\u5f62\u72b6\u7684\u76f8\u4f3c\u6027\uff0c\u800c\u4e0e\u56fe\u50cf\u50cf\u7d20\u5f3a\u5ea6\u503c\u65e0\u5173\u3002\u7136\u800c\uff0c\u5f53\u628a\u8fd9\u4e2a\u7cfb\u6570\u503c\u5e94\u7528\u5230\u5171\u5b9a\u4f4d\u5206\u6790\u65f6\uff0c\u6f5c\u5728\u7684\u8d1f\u503c\u96be\u4ee5\u89e3\u91ca\uff0c\u9700\u8981\u7528\u53e6\u4e00\u79cd\u65b9\u6cd5\u89e3\u91ca\u7ed3\u679c\u3002<\/p>\n<p style=\"text-align: left;\">\u7528\u4e8e\u8ba1\u7b97\u53e6\u4e00\u76f8\u5173\u7cfb\u6570\u7684\u8f83\u7b80\u5355\u7684\u6280\u672f\uff0c\u9700\u8981\u53bb\u6389\u539f\u59cb\u50cf\u7d20\u5f3a\u5ea6\u503c\u548c\u5e73\u5747\u50cf\u7d20\u5f3a\u5ea6\u503c\u8fd9\u4e2a\u5dee\u51cf\u9879\u3002 \u6b63\u5f0f\u5b9a\u4e49\u4e3a Overlap \u7cfb\u6570\uff08 R\uff09 \uff0c\u8fd9\u4e2a\u503c\u8303\u56f4\u4ece 0 \u5230 1\uff0c\u5728\u56fe\u50cf\u5206\u6790\u4e2d\u5bf9\u5f3a\u5ea6\u53d8\u5316\u4e0d\u654f\u611f\u3002 Overlap \u7cfb\u6570\u5b9a\u4e49\u4e3a\uff1a<\/p>\n<p style=\"text-align: left;\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-77479 aligncenter\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/QQ\u622a\u56fe20170424105348-300x111.png\" alt=\"\" width=\"300\" height=\"111\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/QQ\u622a\u56fe20170424105348-300x111.png 300w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/QQ\u622a\u56fe20170424105348.png 457w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/p>\n<p style=\"text-align: left;\">\u5206\u5b50\u662f\u4e24\u4e2a\u901a\u9053\u5f3a\u5ea6\u7684\u4e58\u79ef\u548c\uff0c\u53ea\u6709\u5f53\u540c\u4e00\u4e2a\u50cf\u7d20\u7684\u4e24\u4e2a\u901a\u9053\u503c\u4e0e\u5171\u5b9a\u4f4d\u76f8\u5173\u65f6\uff0c\u5206\u5b50\u624d\u4f1a\u7ed9\u51fa\u4e00\u4e2a\u5f88\u6709\u610f\u4e49\u7684\u503c\uff08\u4e24\u4e2a\u901a\u9053\u7684\u50cf\u7d20\u5f3a\u5ea6\u503c\u90fd\u5927\u4e8e 0\uff09\u3002\u56e0\u6b64\uff0c\u65b9\u7a0b\uff08 2\uff09\u7684\u5206\u5b50\u4e0e\u5171\u5b9a\u4f4d\u7684\u50cf\u7d20\u6570\u6210\u6b63\u6bd4\u3002\u540c\u7406\uff0c Overlap \u65b9\u7a0b\u7684\u5206\u6bcd\u6b63\u6bd4\u4e8e\u56fe\u50cf\u4e24\u4e2a\u901a\u9053\u7ec4\u5206\u7684\u50cf\u7d20\u6570\uff0c\u800c\u4e0d\u8003\u8651\u5171\u5b9a\u4f4d\u662f\u5426\u5b58\u5728\uff08\u6ce8\u610f\uff1a\u7ec4\u5206\u5b9a\u4e49\u4e3a\u901a\u9053 1 \u548c\u901a\u9053 2 \u7ea2\u989c\u8272\u548c\u7eff\u989c\u8272\u7684\u56fe\u6216\u50cf\u7d20\u9635\u5217\uff09\u3002 Overlap \u7cfb\u6570\u7684\u4e00\u4e2a\u4e3b\u8981\u4f18\u70b9\u5c31\u662f\u5bf9\u4e00\u4e2a\u56fe\u50cf\u4e0a\u5404\u79cd\u7ec4\u5206\u7684\u4fe1\u53f7\u5f3a\u5ea6\u5dee\u76f8\u5bf9\u4e0d\u654f\u611f\uff0c\u4fe1\u53f7\u5f3a\u5ea6\u5dee\u7ecf\u5e38\u662f\u7531\u8367\u5149\u56e2\u7684\u6d53\u5ea6\u5dee\u3001\u5149\u6f02\u767d\u3001\u91cf\u5b50\u6548\u7387\u53d8\u5316\u53ca\u975e\u7b49\u6548\u7684\u7535\u5b50\u901a\u9053\u8bbe\u7f6e\u7b49\u4ea7\u751f\u7684\u3002<\/p>\n<p style=\"text-align: left;\">\u4f7f\u7528 Overlap \u7cfb\u6570\u6700\u91cd\u8981\u7684\u7f3a\u70b9\u662f\uff1a\u901a\u9053\u4e4b\u95f4\u7ec4\u5206\u50cf\u7d20\u6570\u6bd4\u4f8b\u4f1a\u5f3a\u70c8\u5f71\u54cdOverlap \u7cfb\u6570\u503c\u3002\u4e3a\u4e86\u51cf\u5c11\u8fd9\u79cd\u4f9d\u8d56\u6027\uff0c Overlap \u7cfb\u6570\u5206\u6210\u4e24\u4e2a\u4e0d\u540c\u7684\u5b50\u7cfb\u6570\uff0c\u770b k(1)\u548c k(2)\uff0c\u4ee5\u4e24\u4e2a\u72ec\u7acb\u7684\u53c2\u91cf\u6765\u8868\u8fbe\u5171\u5b9a\u4f4d\u7684\u7a0b\u5ea6\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-77480 \" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/QQ\u622a\u56fe20170424105456-300x54.png\" alt=\"\" width=\"322\" height=\"58\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/QQ\u622a\u56fe20170424105456-300x54.png 300w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/QQ\u622a\u56fe20170424105456-768x137.png 768w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/QQ\u622a\u56fe20170424105456.png 874w\" sizes=\"(max-width: 322px) 100vw, 322px\" \/> Overlap \u7cfb\u6570 k(1)\u548c k(2)\u63cf\u8ff0\u4e86\u901a\u9053\u4e4b\u95f4\u5f3a\u5ea6\u7684\u5dee\u5f02\uff0c k(1)\u5bf9\u901a\u9053 2\uff08\u7eff\u8272\u4fe1\u53f7\uff09 \u5f3a\u5ea6\u5dee\u654f\u611f\uff0c\u800c k(2)\u7ebf\u6027\u4f9d\u8d56\u4e8e\u901a\u9053 1 \u50cf\u7d20\u7684\u5f3a\u5ea6\u503c\u3002\u56e0\u6b64\uff0c\u73b0\u5728\u63cf\u8ff0\u7684\u65b9\u7a0b\u80fd\u591f\u8bf4\u660e\u91cd\u53e0\u5ea6\uff0c\u4e5f\u80fd\u89e3\u91ca\u989c\u8272\u901a\u9053\u4e4b\u95f4\u7684\u5f3a\u5ea6\u5dee\u5f02\u3002\u5728\u56fe\u50cf\u7684\u5171\u5b9a\u4f4d\u533a\u57df\uff0c\u4e3a\u4e86\u4f30\u8ba1\u5176\u4e2d\u4e00\u4e2a\u989c\u8272\u901a\u9053\u5bf9\u6574\u4e2a\u5171\u5b9a\u4f4d\u8367\u5149\u7684\u8d21\u732e\uff0c\u5b9a\u4e49\u4e86\u53e6\u5916\u4e00\u5957\u5171\u5b9a\u4f4d\u7cfb\u6570 m(1)\u548c m(2)\u3002<\/p>\n<p style=\"text-align: left;\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-77481 aligncenter\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/QQ\u622a\u56fe20170424105600-300x84.png\" alt=\"\" width=\"300\" height=\"84\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/QQ\u622a\u56fe20170424105600-300x84.png 300w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/QQ\u622a\u56fe20170424105600.png 611w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/p>\n<p style=\"text-align: left;\">\u5171\u5b9a\u4f4d\u7cfb\u6570 m(1)\u7528\u4e8e\u63cf\u8ff0\u901a\u9053 1 \u5bf9\u5171\u5b9a\u4f4d\u533a\u57df\u7684\u8d21\u732e\uff0c\u800c\u5171\u5b9a\u4f4d\u7cfb\u6570 m(2)\u7528\u4e8e\u63cf\u8ff0\u901a\u9053 2 \u5bf9\u5171\u5b9a\u4f4d\u533a\u57df\u7684\u8d21\u732e\u3002\u6ce8\u610f\uff0c\u5982 S2(i)\u5927\u4e8e 0 \u65f6\uff0c\u53d8\u91cf S1(i,coloc)\u7b49\u4e8e S1(i)\uff1b\u5bf9\u4e8e\u53d8\u91cf S2(i,coloc)\u4e5f\u662f\u8fd9\u6837\u7684\u3002\u76f8\u5bf9\u4e8e\u6bcf\u4e2a\u8367\u5149\u901a\u9053\u7684\u603b\u8367\u5149\u91cf\u6765\u8bf4\uff0c\u8fd9\u4e9b\u7cfb\u6570\u6b63\u6bd4\u4e8e merge \u56fe\u50cf\u4e2d\u6bcf\u4e2a\u8367\u5149\u901a\u9053\u5171\u5b9a\u4f4d\u8367\u5149\u56e2\u7684\u8367\u5149\u91cf\u3002\u5373\u4f7f\u5f53\u4e24\u4e2a\u8367\u5149\u901a\u9053\u7684\u4fe1\u53f7\u5f3a\u5ea6\u660e\u663e\u5728\u4e0d\u540c\u7684\u6863\u6b21\uff0c\u5171\u5b9a\u4f4d\u7cfb\u6570 m(1)\u548c m(2)\u4e5f\u80fd\u6d4b\u5b9a\u3002<\/p>\n<p style=\"text-align: left;\">\u53e6\u4e00\u5bf9\u5171\u5b9a\u4f4d\u7cfb\u6570\u53ef\u5bf9\u6563\u70b9\u56fe\u4e0a\u5b9a\u4e49\u7684\u611f\u5174\u8da3\u533a\u57df\u5185\u50cf\u7d20\u5f3a\u5ea6\u8303\u56f4\u8fdb\u884c\u8ba1\u7b97\u3002\u7cfb\u6570 M(1)\u7528\u4e8e\u63cf\u8ff0\u901a\u9053 1 \u8367\u5149\u56e2\u5bf9\u5171\u5b9a\u4f4d\u533a\u57df\u7684\u8d21\u732e\uff0c\u7cfb\u6570 M(2)\u7528\u4e8e\u63cf\u8ff0\u901a\u9053 2 \u8367\u5149\u56e2\u5bf9\u5171\u5b9a\u4f4d\u533a\u57df\u7684\u8d21\u732e\u3002<\/p>\n<p style=\"text-align: left;\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-77482 aligncenter\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/QQ\u622a\u56fe20170424105745-300x81.png\" alt=\"\" width=\"300\" height=\"81\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/QQ\u622a\u56fe20170424105745-300x81.png 300w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/QQ\u622a\u56fe20170424105745.png 572w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/p>\n<p style=\"text-align: left;\">\u5f0f\u4e2d\uff0c\u5982 S2(i)\u4f4d\u4e8e\u611f\u5174\u8da3\u533a\u57df\u9608\u503c\u8303\u56f4\u5185\uff0c S1(i,coloc)\u7b49\u4e8e S1(i)\uff1b\u5982 S2(i)\u4ee3\u8868\u7684\u50cf\u7d20\u5728\u611f\u5174\u8da3\u533a\u57df\u9608\u503c\u5916\uff0c S1(i,coloc)\u7b49\u4e8e 0.\u76f8\u4f3c\u5730\uff0c \u5982 S1(i)\u4f4d\u4e8e\u611f\u5174\u8da3\u533a\u57df\u9608\u503c\u8303\u56f4\u5185\uff0c S2(i,coloc)\u7b49\u4e8e S2(i)\uff1b\u5982 S1(i)\u4ee3\u8868\u7684\u50cf\u7d20\u5728\u611f\u5174\u8da3\u533a\u57df\u9608\u503c\u5916\uff0c S2(i,coloc)\u7b49\u4e8e 0. \u6362\u53e5\u8bdd\u8bf4\uff0c\u5bf9\u6bcf\u4e00\u4e2a\u901a\u9053\u6765\u8bf4\uff0c\u5206\u5b50\u4ee3\u8868\u8fd9\u4e2a\u901a\u9053\u6240\u6709\u50cf\u7d20\uff08\u4e14\u6bcf\u4e2a\u50cf\u7d20\u5728\u53e6\u4e00\u4e2a\u901a\u9053\u4e5f\u6709\u5f3a\u5ea6\u503c\uff09\u7684\u5f3a\u5ea6\u548c\uff0c\u800c\u5206\u6bcd\u4ee3\u8868\u8fd9\u4e2a\u901a\u9053\u7684\u6240\u6709\u50cf\u7d20\u7684\u5f3a\u5ea6\u548c\u3002\u76f8\u5bf9\u4e8e\u6bcf\u4e2a\u901a\u9053\u7684\u603b\u8367\u5149\u91cf\u6765\u8bf4\uff0c\u8fd9\u4e9b\u7cfb\u6570\u4e0e\u6bcf\u4e2a\u901a\u9053\u5171\u5b9a\u4f4d\u5bf9\u8c61\u7684\u7684\u8367\u5149\u91cf\u6210\u6b63\u6bd4\u3002<\/p>\n<p style=\"text-align: left;\">\u5927\u591a\u6570\u5546\u4e1a\u5171\u5b9a\u4f4d\u5206\u6790\u8f6f\u4ef6\u90fd\u80fd\u8ba1\u7b97\u4e0a\u9762\u6240\u63cf\u8ff0\u7684\u53c2\u6570\uff0c\u5305\u62ec Pearson\u2032s \u76f8\u5173\u7cfb\u6570\u3001\u603b overlap \u7cfb\u6570\u4ee5\u53ca k(X)\uff0c m(X)\u548c M(x)\u5171\u5b9a\u4f4d\u7cfb\u6570\u3002\u6b64\u5916\uff0c\u8bb8\u591a\u5206\u6790\u8f6f\u4ef6\u5305\u542b\u7684\u7b97\u6cd5\u53ef\u8fdb\u884c\u80cc\u666f\u6821\u6b63\uff0c\u4ea7\u751f\u6574\u5e45\u56fe\u7684\u6563\u70b9\u56fe\uff0c\u4e14\u53ef\u5728\u4e00\u4e2a\u53cc\u901a\u9053\u5408\u6210\u56fe\u6216\u5171\u805a\u7126\u56fe\u7684\u611f\u5174\u8da3\u533a\u57df\u8fdb\u884c\u8ba1\u7b97\u3002\u4ece\u8fd9\u4e9b\u8f6f\u4ef6\u4e2d\u83b7\u5f97\u7684\u6700\u91cd\u8981\u7684\u6570\u636e\u5c31\u662f\u5171\u5b9a\u4f4d\u7cfb\u6570\uff0c\u8fd9\u610f\u5473\u7740\u4fe1\u53f7\u4e4b\u95f4\u91cd\u53e0\u7684\u76f8\u5bf9\u7a0b\u5ea6\u3002\u4f8b\u5982\uff0c\u901a\u9053 1 \u7684\u8367\u5149\u56e2\u5171\u5b9a\u4f4d\u7cfb\u6570\u4e3a 0.75 \u610f\u5473\u7740\u901a\u9053 1 \u4e2d\u542b\u6709\u901a\u9053 2 \u7ec4\u5206\u7684\u5f3a\u5ea6\u503c\u5360\u901a\u9053 1 \u603b\u7684\u5f3a\u5ea6\u503c\u662f 75%\u3002\u8fd9\u662f\u4e00\u4e2a\u76f8\u5bf9\u6bd4\u8f83\u9ad8\u7684\u5171\u5b9a\u4f4d\u7a0b\u5ea6\u3002\u540c\u6837\u5730\uff0c\u5bf9\u901a\u9053 2 \u5171\u5b9a\u4f4d\u7cfb\u6570\u4e3a 0.25 \u610f\u5473\u7740\u660e\u663e\u964d\u4f4e\u7684\u5171\u5b9a\u4f4d\u3002<\/p>\n<p style=\"text-align: left;\"><span style=\"color: #ff0000;\"><strong> \u5171\u5b9a\u4f4d\u5206\u6790\u4e2d\u7684\u5047\u8c61\u53ca\u6837\u54c1\u8003\u8651<\/strong><\/span><\/p>\n<p style=\"text-align: left;\">\u5171\u5b9a\u4f4d\u5206\u6790\u9047\u5230\u7684\u4e00\u4e2a\u6700\u91cd\u8981\u7684\u95ee\u9898\u5c31\u662f\u7531\u53d1\u5c04\u5149\u8c31\u53e0\u52a0\u3001\u81ea\u8367\u5149\uff08\u4e3b\u8981\u5b58\u5728\u4e8e\u7ec4\u7ec7\u6837\u54c1\uff09\u3001\u975e\u7279\u5f02\u6027\u6297\u4f53\u6216\u5408\u6210\u8367\u5149\u67d3\u6599\u67d3\u8272\u5f15\u8d77\u7684\u5149\u8c31\u4e32\u8272\u3002\u8367\u5149\u5171\u632f\u80fd\u91cf\u8f6c\u79fb\u5bf9\u4e8e\u5149\u8c31\u6709\u53e0\u52a0\u7684\u5171\u5b9a\u4f4d\u8367\u5149\u56e2\u6765\u8bf4\u4e5f\u662f\u4e00\u79cd\u6f5c\u5728\u7684\u5047\u8c61\u3002\u5f53\u89c2\u5bdf\u6807\u8bb0\u6709\u7eff\u8272\u3001\u7ea2\u8272\u8367\u5149\u63a2\u9488\u7684\u6837\u54c1\u65f6\uff0c\u4efb\u4f55\u8fd9\u6837\u4e00\u79cd\u5047\u8c61\u90fd\u80fd\u4ea7\u751f\u770b\u8d77\u6765\u662f\u9ec4\u8272\u6216\u6a59\u8272\u7684\u50cf\u7d20\uff0c\u4f46\u8fd9\u79cd\u989c\u8272\u5e76\u4e0d\u6765\u81ea\u5171\u5b9a\u4f4d\u3002<\/p>\n<p style=\"text-align: left;\">\u5f53\u5bf9\u4e24\u79cd\u6216\u4e24\u79cd\u4ee5\u4e0a\u8367\u5149\u67d3\u6599\u6807\u8bb0\u7684\u6837\u54c1\u8fdb\u884c\u6210\u50cf\u65f6\uff0c\u6700\u5e38\u89c1\u7684\u95ee\u9898\u5c31\u662f\u8367\u5149\u4e32\u8272\u95ee\u9898\u3002\u4f8b\u5982\uff0c\u5f53\u7528\u4f20\u7edf\u7684\u7eff\u8272\u548c\u7ea2\u8272\u8367\u5149\u63a2\u9488\u8367\u5149\u7d20\u548c\u7f57\u4e39\u660e\u8fdb\u884c\u53cc\u6807\u65f6\uff0c\u4e32\u8272\u53ea\u6709\u7528\u6700\u4f18\u7684\u8367\u5149\u6ee4\u8272\u955c\u7ec4\u624d\u80fd\u964d\u4f4e\uff0c\u4f46\u7edd\u4e0d\u4f1a\u5b8c\u5168\u53bb\u6389\u3002\u8fd9\u662f\u56e0\u4e3a\u8fd9\u6837\u4e00\u4e2a\u4e8b\u5b9e\uff1a\u8fd9\u4e9b\u67d3\u6599\u90fd\u6709\u5f88\u5bbd\u7684\u5438\u6536\u548c\u53d1\u5c04\u5149\u8c31\uff0c\u5149\u8c31\u4e4b\u95f4\u6709\u660e\u663e\u7684\u91cd\u53e0\u3002\u56e0\u6b64\uff0c\u6fc0\u53d1\u8367\u5149\u7d20\u7684\u6c29\u79bb\u5b50\u6fc0\u5149\u5668\u7684 488nm \u7ebf\u4e5f\u80fd\u6fc0\u53d1\u7f57\u4e39\u660e\uff0c\u5c3d\u7ba1\u6fc0\u53d1\u7a0b\u5ea6\u8f83\u4f4e\u3002\u800c\u4e14\uff0c\u5728\u4e3a\u7f57\u4e39\u660e\u9884\u8bbe\u7684\u5149\u7535\u500d\u589e\u7ba1\u901a\u9053\u6216\u5bbd\u573a\u6ee4\u8272\u955c\u5957\u7ec4\u4e2d\u4e5f\u4f1a\u68c0\u6d4b\u5230\u8367\u5149\u7d20\u7684\u8367\u5149\u53d1\u5c04\uff0c\u751a\u81f3\u5728\u6ca1\u6709\u5171\u5b9a\u4f4d\u7684\u5730\u65b9\uff0c\u4ea7\u751f\u770b\u8d77\u6765\u662f\u9ec4\u8272\u6216\u6a59\u8272\u7684\u50cf\u7d20\u3002\u8981\u7528\u8fd9\u4e9b\u67d3\u6599\u6216\u7c7b\u4f3c\u67d3\u6599\u8fdb\u884c\u5171\u5b9a\u4f4d\u5b9e\u9a8c\u65f6\uff0c\u4e32\u8272\u95ee\u9898\u5fc5\u987b\u5b8c\u5168\u6d88\u9664\u3002<\/p>\n<p style=\"text-align: left;\">\u6210\u63a2\u9488\u514b\u670d\uff0c\u6bd4\u5982 Alexa Fluor \u7cfb\u5217\u6216 Cy \u7cfb\u5217\u67d3\u6599\u3002\u8fd9\u4e9b\u4e13\u95e8\u8bbe\u8ba1\u7684\u6709\u673a\u5206\u5b50\u8868\u73b0\u51fa\u5f88\u7a84\u7684\u53d1\u5c04\u5149\u8c31\uff08\u4e0e\u4f20\u7edf\u63a2\u9488\u76f8\u6bd4\uff09\u3001\u4e0e\u5f27\u5149\u706f\u548c\u6fc0\u5149\u7ebf\u76f8\u5339\u914d\u7684\u8f83\u5927\u7684\u5438\u6536\u7cfb\u6570\u3001\u8f83\u9ad8\u7684\u91cf\u5b50\u4ea7\u7387\u3001\u964d\u4f4e\u7684\u5149\u6f02\u767d,\u4e14\u8367\u5149\u53d1\u5c04\u5bf9\u73af\u5883\u53d8\u91cf\u7684\u4f9d\u8d56\u6027\u8f83\u4f4e\u3002\u6b64\u5916\uff0c\u8fd9\u4e9b\u5148\u8fdb\u63a2\u9488\u7684\u6fc0\u53d1\u5149\u8c31\u7ebf\u6a2a\u8de8\u5927\u7ea6 400nm\uff0c\u4ece\u7d2b\u5916\u5230\u8fd1\u7ea2\u5916\u6709\u4e00\u4e2a\u5f88\u5bbd\u7684\u9009\u62e9\uff0c\u4ee5\u5339\u914d\u7167\u660e\u5149\u6e90\uff0c\u4f7f\u5f97\u53d1\u5c04\u5149\u8c31\u53e0\u52a0\u6700\u5c0f\u3002<\/p>\n<p style=\"text-align: left;\">\u81ea\u53d1\u8367\u5149\uff08\u5bf9\u7532\u919b\u56fa\u5b9a\u7684\u7ec4\u7ec7\u6837\u54c1\u5c24\u4e3a\u663e\u8457\uff09\u4ea7\u751f\u7684\u95ee\u9898\u5728\u8868\u73b0\u4e0a\u4e0e\u4e32\u8272\u7c7b\u4f3c\u3002\u6709\u81ea\u53d1\u8367\u5149\u7684\u6837\u54c1\u6fc0\u53d1\u540e\u7ecf\u5e38\u4f1a\u5728\u5176\u4ed6\u901a\u9053\u68c0\u6d4b\u51fa\u8367\u5149\u53d1\u5c04\uff0c\u4f7f\u5f97\u5230\u7684\u7167\u7247\u770b\u8d77\u6765\u6709\u5171\u5b9a\u4f4d\u8367\u5149\u56e2\u3002\u5982\u7528\u6297\u4f53\u548c\u5408\u6210\u8367\u5149\u56e2\u5bf9\u80cc\u666f\u8fc7\u5ea6\u67d3\u8272\uff0c\u4e5f\u4f1a\u5728\u4e24\u4e2a\u8367\u5149\u56e2\u975e\u7279\u5f02\u6027\u6807\u8bb0\u660e\u663e\u7684\u5730\u65b9\u4ea7\u751f\u770b\u8d77\u6765\u50cf\u5171\u5b9a\u4f4d\u7684\u56fe\u50cf\u3002\u8fd9\u4e2a\u5047\u8c61\u53ef\u4ee5\u901a\u8fc7\u8ba4\u771f\u5730\u5236\u5907\u6837\u54c1\u3001\u7528\u5408\u9002\u7684 control \u76d1\u6d4b\u5b9e\u9a8c\u65b9\u6848\u6765\u907f\u514d\u6216\u660e\u663e\u964d\u4f4e\u3002<\/p>\n<p style=\"text-align: left;\">\u53ea\u6709\u5f53\u6240\u6709\u7684\u4e32\u8272\u3001\u81ea\u53d1\u8367\u5149\u3001\u975e\u7279\u5f02\u6027\u8367\u5149\u95ee\u9898\u90fd\u6d88\u9664\u6389\u540e\uff0c\u5171\u5b9a\u4f4d\u7684\u7814\u7a76\u624d\u662f\u51c6\u786e\u7684\u3002\u5728\u6fc0\u5149\u626b\u63cf\u5171\u805a\u7126\u663e\u5fae\u955c\u4e2d\uff0c\u6700\u6709\u6548\u7684\u65b9\u6cd5\u5c31\u662f\u5229\u7528\u5e8f\u5217\u6fc0\u53d1\uff0c\u5e76\u7528\u7a84\u7684\u5e26\u901a\u53d1\u5c04\u6ee4\u8272\u7247\u6216\u7a84\u7684\u72ed\u7f1d\u5bbd\u5ea6\uff08\u5149\u8c31\u578b\uff09\u6536\u96c6\u53d1\u5c04\u8367\u5149\u3002\u540c\u65f6\uff0c\u5e94\u8be5\u68c0\u67e5\u5355\u6807\u7684 control \u6837\u54c1\uff0c\u4ee5\u786e\u4fdd\u5b8c\u5168\u6d88\u9664\u4e86\u4e32\u8272\uff0c\u672a\u67d3\u8272\u7684 control \u5728\u76d1\u6d4b\u81ea\u8367\u5149\u65b9\u9762\u662f\u5f88\u6709\u6548\u7684\u3002<\/p>\n<p style=\"text-align: left;\"><a href=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/06\/\u56fe5.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-85841\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/06\/\u56fe5.png\" alt=\"\" width=\"650\" height=\"494\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/06\/\u56fe5.png 650w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/06\/\u56fe5-300x228.png 300w\" sizes=\"(max-width: 650px) 100vw, 650px\" \/><\/a><\/p>\n<p style=\"text-align: center;\"><strong>\u56fe5<\/strong><\/p>\n<p style=\"text-align: left;\">\u6fc0\u5149\u626b\u63cf\u5171\u805a\u7126\u663e\u5fae\u955c\u4e2d\u5404\u79cd\u6837\u54c1\u4e32\u8272\u7684\u95ee\u9898\u53ca\u5176\u6821\u6b63\u5728\u56fe 5 \u4e2d\u663e\u793a\u3002\u56fe 5\uff08 a\uff09\u4e2d\u7684\u7ea4\u7ef4\u539f\u7ec6\u80de\uff0c Alexa Fluor488 \u7eff\u8272\u8367\u5149\u4e32\u8272\u8fdb\u5165 Mito Tracker \u7ea2\u8272\u901a\u9053\uff0c\u5f53\u6837\u54c1\u7528 488 \u6fc0\u5149\u548c 543 \u6fc0\u5149\u540c\u65f6\u626b\u63cf\u65f6\uff0c\u4f1a\u4ea7\u751f\u9ec4\u8272\u7684\u808c\u52a8\u86cb\u767d\u4e1d\u3002\u5e8f\u5217\u626b\u63cf\u548c\u68c0\u6d4b\uff08\u56fe 5d\uff09\u6d88\u9664\u4e86\u4e32\u8272\u5f71\u54cd\u3002\u540c\u6837\u5730\uff0c\u5728\u7528 Cy3 \u548c\u6838\u63a2\u9488 DRAQ5 \u5bf9\u8001\u9f20\u5927\u80a0\u539a\u5207\u7247\u8fdb\u884c\u540c\u65f6\u626b\u63cf\u65f6\uff08\u56fe 5b\uff09\uff0c\u4e5f\u4f1a\u53d1\u751f\u4e32\u8272\u3002\u5e8f\u5217\u626b\u63cf\u53ef\u4ee5\u53bb\u6389\u4e32\u8272\u5047\u8c61\uff08\u56fe 5e\uff09\uff0c\u800c\u4e32\u8272\u5047\u8c61\u4f1a\u9020\u6210\u865a\u5047\u7684\u5171\u5b9a\u4f4d\u3002<\/p>\n<p style=\"text-align: left;\">\u5f53\u591a\u6807\u6837\u54c1\u7528\u4e24\u79cd\u4ee5\u4e0a\u7684\u6fc0\u5149\u626b\u63cf\u65f6\uff0c\u4e32\u8272\u95ee\u9898\u5728\u597d\u51e0\u4e2a\u901a\u9053\u90fd\u4f1a\u89c2\u5bdf\u5230\uff0c\u56fe 5\uff08 c\uff09\u548c\u56fe 5(f)\u663e\u793a\u4e86\u9f20\u8111\u7684\u4e00\u4e2a\u539a\u5207\u7247\uff0c\u7528 Alexa Fluor488 \u6807\u8bb0\u795e\u7ecf\u4e1d\u3001\u7528 Alexa Fluor 568 \u6807\u8bb0\u795e\u7ecf\u80f6\u8d28\u7ea4\u7ef4\u9178\u6027\u86cb\u767d(GFAP)\uff0c\u7528 DRAQ5 \u67d3\u6838\u3002\u5f53\u6837\u54c1\u540c\u65f6\u7528 3 \u4e2a\u6fc0\u5149\u626b\u63cf\u65f6\uff08\u56fe 5c\uff09\uff0c\u4e32\u8272\u4f1a\u5728\u7b2c\u4e8c\u548c\u7b2c\u4e09\u4e2a\u901a\u9053\u53d1\u751f\uff0c\u5728\u6837\u54c1\u7684\u4e2d\u95f4\u4e1d\u91cc\u5c31\u610f\u5473\u7740\u6709\u53ef\u80fd\u7684\u5171\u5b9a\u4f4d\u3002\u7136\u800c\uff0c\u82e5\u5f00\u59cb\u7528\u5e8f\u5217\u626b\u63cf\u5e76\u6536\u96c6\u6570\u636e\u65f6\uff0c\u795e\u7ecf\u4e1d\u53ea\u51fa\u73b0\u5728\u5b83\u4eec\u5404\u81ea\u7684\u901a\u9053\u91cc\uff08\u56fe 5f\uff09\uff0c\u6d88\u9664\u4e86\u8fd9\u4e9b\u7ed3\u6784\u4e2d\u8367\u5149\u56e2\u5171\u5b9a\u4f4d\u7684\u53ef\u80fd\u3002\u8367\u5149\u5171\u632f\u80fd\u91cf\u8f6c\u79fb(FRET)\uff0c\u7406\u8bba\u4e0a\u53ef\u4ee5\u6d4b\u91cf\u4f4d\u7f6e\u79bb\u5f97\u5f88\u8fd1\u7684\u4e24\u4e2a\u8367\u5149\u56e2\u7684\u8ddd\u79bb\uff0c\u4e5f\u662f\u76d1\u6d4b\u5171\u5b9a\u4f4d\u7684\u4e00\u4e2a\u6f5c\u5728\u6709\u7528\u7684\u5de5\u5177\u3002\u7136\u800c\uff0c\u5728\u5171\u5b9a\u4f4d\u5206\u6790\u4e2d\u8fd9\u4e2a\u73b0\u8c61\u7ecf\u5e38\u4f1a\u5bfc\u81f4\u4e00\u4e9b\u5047\u8c61\uff0c\u9664\u975e\u5728\u5b9e\u9a8c\u7684\u8bbe\u8ba1\u8fc7\u7a0b\u4e2d\uff0c\u5bf9 FRET \u7684\u4e00\u4e9b\u53c2\u6570\u505a\u4e86\u5f88\u597d\u5730\u7406\u89e3\u5e76\u8ba4\u771f\u8fdb\u884c\u4e86\u8003\u8651\u3002\u5c24\u5176\u662f\u5728\u7b2c\u4e00\u4e2a\u8367\u5149\u56e2\u7684\u53d1\u5c04\u5149\u8c31\u548c\u7b2c\u4e8c\u4e2a\u8367\u5149\u56e2\u7684\u6fc0\u53d1\u5149\u8c31\u91cd\u53e0\u7684\u5730\u65b9\uff0c\u7814\u7a76\u8005\u5e94\u8be5\u7279\u522b\u5173\u6ce8\u3002\u8fd9\u4e9b\u6837\u54c1\u4e2d\u7684\u80fd\u91cf\u8f6c\u79fb\u8868\u73b0\u4e3a\uff1a\u7b2c\u4e00\u4e2a\u8367\u5149\u56e2\u7684\u8367\u5149\u53d1\u5c04\u610f\u5916\u5730\u964d\u4f4e\u540c\u65f6\u4f34\u968f\u7740\u7b2c\u4e8c\u4e2a\u8367\u5149\u56e2\u7684\u8367\u5149\u53d1\u5c04\u610f\u5916\u5730\u589e\u52a0\u3002\u7d27\u5bc6\u76f8\u5173\u7684\u8367\u5149\u56e2\u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u53ef\u5bfc\u81f4\u53d1\u5c04\u8367\u5149\u4fe1\u53f7\u7684\u6dec\u706d\uff0c\u8fd9\u4e0e\u73af\u5883\u6761\u4ef6\u6709\u5173\u3002<\/p>\n<p style=\"text-align: left;\">\u5171\u5b9a\u4f4d\u5206\u6790\u4e2d\u8bb8\u591a\u6f5c\u5728\u7684\u95ee\u9898\u90fd\u53ef\u901a\u8fc7\u4ed4\u7ec6\u5173\u6ce8\u6837\u54c1\u5236\u5907\u6280\u672f\u6765\u89c4\u907f\u3002\u6b63\u5982\u4e0a\u9762\u8ba8\u8bba\u7684\uff0c\u8367\u5149\u63a2\u9488\u5e94\u8be5\u9009\u62e9\u53d1\u5c04\u5149\u8c31\u5206\u79bb\u6bd4\u8f83\u5927\u7684\u4e14\u5177\u6709\u6700\u5c0f\u53e0\u52a0\u7684\u7ec4\u5408\u3002\u5982\u679c\u53ef\u80fd\u7684\u8bdd\uff0c\u9009\u62e9\u7eff\u989c\u8272\u7684\u5177\u6709\u7a84\u53d1\u5c04\u7684\u8367\u5149\u56e2\uff08\u6bd4\u5982 Alexa Fluor \u7cfb\u5217\u6216\u91cf\u5b50\u70b9\uff09\u53ca\u7ea2\u989c\u8272\u7684\u5728\u6df1\u7ea2\u533a\u6216\u8fd1\u7ea2\u5916\u533a\u6709\u53d1\u5c04\u7684\u8367\u5149\u56e2\u3002\u539f\u53d1\u6027\u548c\u7ee7\u53d1\u6027\u6297\u4f53\u5fc5\u987b\u68c0\u6d4b\u4ea4\u53c9\u6d3b\u6027\u53ca\u975e\u7279\u5f02\u6027\u80cc\u666f\u67d3\u8272\u3002\u5408\u6210\u8367\u5149\u56e2\u53ca\u6807\u8bb0\u7684\u7ee7\u53d1\u6027\u6297\u4f53\u7684\u6d53\u5ea6\u5e94\u8be5\u4f18\u5316\u4ee5\u786e\u4fdd\u76f8\u4f3c\u7684\u4eae\u5ea6\uff0c\u5c24\u5176\u662f\u5f53\u5bf9\u8c61\u4e30\u5ea6\u6839\u672c\u4e0d\u540c\u65f6\u3002\u5f71\u54cd\u8367\u5149\u56e2\u4eae\u5ea6\u7684\u56e0\u7d20\u5305\u62ec\u6fc0\u53d1\u6548\u7387\u3001\u91cf\u5b50\u4ea7\u7387\u3001\u6d88\u5149\u7cfb\u6570\u3001\u6d53\u5ea6\u53ca\u73af\u5883\u56e0\u7d20\uff0c\u5982 pH\u503c\u3001\u79bb\u5b50\u6d53\u5ea6\u3001\u758f\u6c34\u6027\u53ca\u7c98\u6027\u3002\u5bf9\u6bcf\u4e00\u4e2a\u8367\u5149\u56e2\u90fd\u5e94\u5355\u72ec\u5236\u5907 control \u6837\u54c1\uff0c\u5728\u4e0d\u67d3\u8272\u7684\u60c5\u51b5\u4e0b\uff0c\u5206\u522b\u5206\u6790\u4e32\u8272\u548c\u81ea\u8367\u5149\u3002\u4ed4\u7ec6\u6ce8\u610f\u7740\u8272\u8fc7\u7a0b\u4e2d\u7684\u5fae\u5c0f\u7ec6\u8282\uff0c\u5c31\u4f1a\u964d\u4f4e\u4f7f\u7528\u56fe\u50cf\u5904\u7406\u8f6f\u4ef6\u6062\u590d\u6570\u5b57\u56fe\u50cf\u7684\u5fc5\u8981\u6027\u3002<\/p>\n<p style=\"text-align: left;\"><span style=\"color: #ff0000;\"><strong> \u5171\u5b9a\u4f4d\u5206\u6790\u7684\u5b9e\u9645\u60c5\u51b5<\/strong><\/span><\/p>\n<p style=\"text-align: left;\">\u5728\u5206\u6790\u590d\u6742\u7684\u8367\u5149\u56fe\u50cf\u65f6\uff0c\u4f7f\u7528\u4f2a\u5f69\u8272\u7ec4\u5408\u5f88\u6709\u7528\uff0c\u8fd9\u5728\u4e0a\u9762\u5df2\u7ecf\u8ba8\u8bba\u8fc7\u4e86\u3002\u901a\u5e38\uff0c\u9009\u62e9\u4e24\u4e2a\u5f69\u8272\u901a\u9053\uff0c\u6700\u5e38\u7528\u7684\u662f\u7eff\u8272\u548c\u7ea2\u8272\u3002\u56e0\u6b64\uff0c\u53e0\u52a0\u533a\u57df\u663e\u793a\u9ec4\u8272\u3002\u5176\u4ed6\u989c\u8272\u5bf9\uff0c\u6bd4\u5982\u84dd\u8272\u3001\u7eff\u8272\uff08\u4ea7\u751f\u9752\u8272\uff09\uff0c\u84dd\u8272\u3001\u7ea2\u8272\uff08\u4ea7\u751f\u7d2b\u8272\uff09\u4e5f\u4f1a\u7528\u5230\uff0c\u4f46\u56e0\u4e3a\u53cd\u5c04\u7684\u84dd\u5149\u5bf9\u4eba\u773c\u7075\u654f\u5ea6\u4f4e\uff0c\u8fd9\u4e9b\u989c\u8272\u5bf9\u5728\u5b9e\u9645\u89c2\u5bdf\u4e2d\u5f88\u5c11\u4f7f\u7528\u3002\u4f46\u5728\u8bb8\u591a\u60c5\u51b5\u4e0b\uff0c\u5c24\u5176\u662f\u4e09\u8272\u56fe\u50cf\uff0c\u4e0d\u53ef\u907f\u514d\u4f1a\u4f7f\u7528\u84dd\u8272\u4f2a\u5f69\u8272\u3002<\/p>\n<p style=\"text-align: left;\">\u91c7\u96c6\u56fe\u50cf\u53ca\u663e\u793a\u53c2\u6570\u65b9\u9762\uff0c\u5e94\u8be5\u91c7\u7528\u4e00\u79cd\u6807\u51c6\u4ee5\u4fbf\u4f7f\u5408\u6210\u56fe\u5bf9\u6bcf\u4e00\u4e2a\u8367\u5149\u4fe1\u53f7\u663e\u793a\u540c\u6837\u7684\u52a8\u6001\u8303\u56f4\u548c\u8865\u507f\u3002\u4e8b\u5b9e\u4e0a\uff0c\u6807\u51c6\u7684\u5171\u805a\u7126\u663e\u5fae\u955c\u64cd\u4f5c\u5c06\u8fd9\u79cd\u65b9\u6cd5\u89c6\u4e3a\u65e5\u5e38\u6837\u54c1\u5206\u6790\u65b9\u6cd5\u3002\u4e24\u4e2a\u56fe\u50cf merge \u65f6\uff0c\u5982\u679c\u6536\u96c6\u5230\u7684\u5149\u5b50\u8db3\u591f\uff0c\u53e0\u52a0\u7684\u7ea2\u8272\u7eff\u8272\u4fe1\u53f7\u5c31\u4f1a\u4ea7\u751f\u660e\u786e\u7684\u4eae\u9ec4\u8272\u3002\u5728\u5149\u5b50\u6781\u5c11\u7684\u5b9e\u9a8c\u4e2d\uff0c\u5171\u5b9a\u4f4d\u8367\u5149\u56e2\u4f1a\u4ea7\u751f\u6697\u9ec4\u8272\uff0c\u800c\u5bf9\u80cc\u666f\u5177\u6709\u76f8\u540c\u8d21\u732e\u7684\u7eff\u8272\u548c\u7ea2\u8272\u4f1a\u663e\u793a\u68d5\u8272\u3002<\/p>\n<p style=\"text-align: left;\">\u6bcf\u4e00\u4e2a\u901a\u9053\u7684 offset \u548c gain \u90fd\u5e94\u8be5\u5355\u72ec\u8c03\u8282\uff08\u8bbe\u7f6e\u80cc\u666f\u4e3a 0\uff0c\u9971\u548c\u4e3a 4095\uff09\uff0c\u4ee5\u4fbf\u6bcf\u4e00\u4e2a\u8367\u5149\u56e2\u90fd\u663e\u793a\u5728\u5b8c\u6574\u7684 12 \u4f4d\u8303\u56f4\u91cc\u3002\u7136\u540e\uff0c\u5bf9\u6bcf\u4e2a\u56fe\u50cf\u8fdb\u884c\u5355\u72ec\u5904\u7406\u3002\u5c3d\u7ba1\u8fd9\u662f\u91c7\u96c6\u548c\u663e\u793a\u591a\u8272\u56fe\u50cf\u7684\u4e00\u4e2a\u5f88\u65b9\u4fbf\u7684\u65b9\u6cd5\uff0c\u4f46\u6837\u54c1\u4e2d\u4e24\u4e2a\u4fe1\u53f7\u7684\u5b9e\u9645\u76f8\u5bf9\u5f3a\u5ea6\u6ca1\u6cd5\u6d4b\u5b9a\uff0c\u56e0\u4e3a\u6bcf\u4e2a\u4fe1\u53f7\u7684\u91c7\u96c6\u90fd\u662f\u4e3a\u4e86\u6ee1\u8db3\u6574\u4e2a 12 \u4f4d\u7684\u56fe\u50cf\u6df1\u5ea6\u3002\u56e0\u6b64\uff0c\u67d0\u4e00\u4e2a\u989c\u8272\u901a\u9053\u76f8\u5bf9\u4fe1\u53f7\u5f3a\u5ea6\u7684\u5206\u6790\u8981\u6c42\u5404\u4e2a\u901a\u9053\u4ee5\u540c\u6837\u7684\u91c7\u96c6\u53c2\u6570\u914d\u7f6e\uff0c\u6bd4\u5982\u5149\u7535\u500d\u589e\u7ba1\u7535\u538b\u3001\u589e\u76ca\u548c\u8865\u507f\u7b49\u3002<\/p>\n<p style=\"text-align: left;\">\u5982\u679c\u56fe\u50cf\u91c7\u96c6\u548c\u56fe\u50cf\u5904\u7406\u6280\u672f\u6ca1\u6709\u5e73\u8861\u597d\uff0c\u5728 merge \u7684\u5f69\u8272\u56fe\u4e2d\uff0c\u5c31\u4f1a\u7531\u4e8e\u91c7\u96c6\u8fc7\u7a0b\u5149\u7535\u500d\u589e\u7ba1\u4e0d\u5408\u9002\u7684 Gain\u3001 offset \u914d\u7f6e\u53ca\u56fe\u50cf\u5904\u7406\u8fc7\u7a0b\u4e2d\u5f3a\u5ea6\u76f4\u65b9\u56fe\u7684\u6781\u7aef\u62c9\u4f38\u800c\u5bf9\u5171\u5b9a\u4f4d\u505a\u51fa\u4e0d\u51c6\u786e\u7684\u89e3\u91ca\u3002\u4e3e\u4e2a\u4f8b\u5b50\uff0c\u5982\u679c offset \u503c\u8fc7\u9ad8\uff0c\u5c31\u4f1a\u53d1\u751f\u5f88\u660e\u663e\u7684\u989c\u8272\u5206\u79bb\uff0c\u5728\u4f4e offset \u503c\u4e0b\uff0c\u5728\u540c\u6837\u7684\u7ed3\u6784\u4e0b\uff0c\u89c2\u5bdf\u5230\u7684\u5bf9\u6bd4\u5ea6\u5c31\u964d\u4f4e\u3002\u5bf9\u4e8e\u4e00\u4e9b\u5173\u952e\u5e94\u7528\uff0c ratio \u6210\u50cf\u6280\u672f\u53ef\u7528\u4e8e\u533a\u5206\u4e24\u4e2a\u4fe1\u53f7\u662f\u5171\u5b9a\u4f4d\u8fd8\u662f\u53ea\u662f\u90e8\u5206\u53e0\u52a0\u3002<\/p>\n<p style=\"text-align: left;\"><a href=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/06\/\u56fe6.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-85842\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/06\/\u56fe6.png\" alt=\"\" width=\"640\" height=\"485\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/06\/\u56fe6.png 640w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/06\/\u56fe6-300x227.png 300w\" sizes=\"(max-width: 640px) 100vw, 640px\" \/><\/a><\/p>\n<p style=\"text-align: center;\"><strong>\u56fe6<\/strong><\/p>\n<p style=\"text-align: left;\">\u5bf9\u5b8c\u5168\u6821\u51c6\u597d\u7684\u8367\u5149\u6210\u50cf\u7cfb\u7edf\uff0c\u5f53\u7528\u4e0d\u540c\u7684\u6ee4\u8272\u955c\u7ec4\u65f6\uff0c\u6837\u54c1\u4e0a\u4e00\u4e2a\u70b9\u5728\u68c0\u6d4b\u5668\u4e0a\u7cbe\u786e\u6210\u50cf\u4e3a\u4e00\u4e2a\u70b9\uff0c\u4e5f\u5c31\u662f\u50cf\u7d20\u5bf9\u50cf\u7d20\u3002\u7136\u800c\uff0c\u4e0d\u540c\u989c\u8272\u7684\u901a\u9053 merge \u65f6\uff0c\u7269\u955c\u7684\u8272\u5dee\u6821\u6b63\u4e0d\u591f\u3001\u6ee4\u955c\u5149\u8def\u6ca1\u6709\u5b8c\u5168\u5bf9\u51c6\u90fd\u4f1a\u4f7f\u5f97\u8367\u5149\u4fe1\u53f7\u4e4b\u95f4\u7684\u8bb0\u5f55\u6709\u5dee\u9519\u3002\u5bf9\u5177\u6709\u590d\u6742\u56fe\u6848\u7684\u56fe\u50cf\u6216\u660e\u6697\u4fe1\u53f7\u76f8\u6df7\u7684\u56fe\u50cf\uff0c\u8fd9\u4e2a\u53ef\u80fd\u5c31\u68c0\u6d4b\u4e0d\u5230\u3002\u4f1a\u5f97\u51fa\u8fd9\u6837\u7684\u7ed3\u8bba\uff1a\u5728\u7ed3\u6784\u4e2d\u7684\u4fe1\u53f7\u5206\u5e03\u8981\u4e48\u662f\u660e\u663e\u4e0d\u540c\uff0c\u8981\u4e48\u662f\u90e8\u5206\u53e0\u52a0\u7684\u3002<\/p>\n<p style=\"text-align: left;\">\u5728 merge \u56fe\u50cf\u7684\u5904\u7406\u8fc7\u7a0b\u4e2d\uff0c\u4f4d\u79fb\u95ee\u9898\u53ef\u7528\u8bb8\u591a\u8f6f\u4ef6\u5305\u901a\u8fc7 panning \u64cd\u4f5c\u6062\u590d\u539f\u59cb\u8bb0\u5f55\u3002\u901a\u8fc7 panning \u64cd\u4f5c\u6821\u6b63\u4e00\u7cfb\u5217\u4e0d\u540c\u989c\u8272\u56fe\u7684\u8fc7\u7a0b\u4e2d\uff0c\u9700\u8981\u6837\u54c1\u4e0a\u6709\u4e00\u4e2a\u56fa\u5b9a\u7684\u53c2\u8003\u70b9\uff0c\u8fd9\u4e2a\u53c2\u8003\u70b9\u5728\u6bcf\u4e00\u5c42\u56fe\u4e0a\u90fd\u6709\u3002\u5982\u4e0d\u5b58\u5728\u591a\u6807\u7684\u6837\u54c1\u53c2\u8003\u70b9\uff0c\u5c31\u5c06\u591a\u8272\u7684\u8367\u5149\u5fae\u7403\u7a00\u91ca\u540e\u52a0\u5165\u6837\u54c1\u4e2d\uff0c\u7528\u76d6\u73bb\u7247\u8fdb\u884c\u5c01\u88c5\u524d\uff0c\u5728\u6bcf\u4e2a\u89c6\u91ce\u4e2d\u90fd\u4f1a\u6709\u51e0\u4e2a\u73e0\u5b50\u3002\u5bf9\u4e8e\u4e00\u4e9b\u82db\u523b\u7684\u5e94\u7528\uff0c\u51e0\u4e2a\u5e26\u901a\u4e8c\u8272\u955c\u548c\u53d1\u5c04\u6ee4\u8272\u7247\u53ef\u4e0e\u4e0d\u540c\u7684\u6fc0\u5149\u5668\u6216\u8367\u5149\u56e2\u7279\u5b9a\u7684\u6fc0\u53d1\u6ee4\u8272\u7247\u4e00\u8d77\u4f7f\u7528\u3002\u8fd9\u79cd\u6ee4\u5149\u955c\u7ec4\u7684\u914d\u7f6e\u4e00\u822c\u7528\u5728\u5171\u805a\u7126\u663e\u5fae\u955c\u4e2d\uff0c\u5f53\u5bf9\u989c\u8272\u6821\u6b63\u8981\u6c42\u82db\u523b\u65f6\uff0c\u4e5f\u53ef\u7528\u4e8e\u5bbd\u573a\u8367\u5149\u663e\u5fae\u955c\u3002<\/p>\n<p style=\"text-align: left;\">\u56fe 6 \u663e\u793a\u4e86\u4e0a\u9762\u8ba8\u8bba\u7684\u51e0\u4e2a\u5e38\u89c1\u95ee\u9898\uff0c\u591a\u6807\u6837\u54c1\uff08\u6ce8\u610f\uff1a\u56fe 6 \u4e2d\u6240\u6709\u8367\u5149\u56e2\u90fd\u53ea\u663e\u793a\u7eff\u8272\u548c\u7ea2\u8272\u4e24\u79cd\u4f2a\u5f69\uff09\u7ecf\u5e38\u4f1a\u59a8\u788d\u5171\u5b9a\u4f4d\u7684\u51c6\u786e\u5206\u6790\u3002\u7528\u589e\u5f3a\u9ec4\u8272\u8367\u5149\u86cb\u767d\u878d\u5408\u8fc7\u6c27\u5316\u9176\u8f6c\u67d3\u4eba\u7c7b\u5973\u6027\u9aa8\u8089\u7624\u4e0a\u76ae\u7ec6\u80de\uff0c\u76ee\u6807\u5bf9\u8c61\u662f\u7f29\u6c28\u9178\u5e8f\u5217\uff08\u53d1\u7eff\u8272\u8367\u5149\uff09\uff0c\u968f\u540e\u7528\u514d\u75ab\u8367\u5149\u7684\u65b9\u6cd5\uff0c\u7528\u4e8c\u6b21\u6297\u4f53\u6807\u8bb0 Alexa Fluor568(\u53d1\u7ea2\u5149)\uff0c\u76ee\u6807\u5bf9\u8c61\u662f\u8fc7\u6c27\u5316\u9176\u819c\u86cb\u767d\uff08 PMP-70\uff09\uff0c\u663e\u793a\u5728\u56fe 6\uff08 a\uff09\u548c\u56fe 6\uff08 d\uff09\u4e2d\u3002\u56fe 6\uff08 a\uff09\u4e2d\u7684\u80cc\u666f\u592a\u9ed1\uff0c\u81f4\u4f7f\u6807\u8bb0\u7684\u9ec4\u8272\u8367\u5149\u86cb\u767d\u5f3a\u5ea6\u964d\u4f4e\u3002\u8fd9\u79cd\u9519\u8bef\u4f7f\u5171\u5b9a\u4f4d\u5206\u6790\u53d1\u751f\u504f\u79fb\uff0c\u5bf9\u7eff\u8272\u901a\u9053\u4e2d\u91cd\u53e0\u7684\u50cf\u7d20\u4eba\u4e3a\u4ea7\u751f\u4e86\u8f83\u4f4e\u7684\u68c0\u6d4b\u91cf\u3002\u5408\u9002\u7684\u56fe\u663e\u793a\u5728\u56fe 6\uff08 d\uff09\uff0c\u663e\u793a\u4e86\u660e\u663e\u8f83\u9ad8\u7684\u5171\u5b9a\u4f4d\u7a0b\u5ea6\u3002<\/p>\n<p style=\"text-align: left;\">\u7315\u7334\u80be\u4e0a\u76ae\u7ec6\u80de\u7684\u7ebf\u7c92\u4f53\u7f51\u7edc\u7528 EYFP \u548c HcRed1 \u8367\u5149\u86cb\u767d\uff08\u6b64\u86cb\u767d\u542b\u6709\u7f29\u6c28\u9178\u5e8f\u5217\uff0c\u76ee\u6807\u5bf9\u8c61\u662f\u7ebf\u7c92\u4f53\uff09\u8f6c\u67d3\u3002\u6210\u50cf\u8fc7\u7a0b\u4e2d\uff0c\u975e\u5e38\u4eae\u7684 EYFP \u5927\u5927\u5730\u906e\u76d6\u4e86HcRed1 \u53d1\u5c04\u7684\u8367\u5149\u4fe1\u53f7\uff0c\u5f53\u68c0\u6d4b\u901a\u9053\u7684\u7535\u538b\u3001\u589e\u76ca\u548c\u8865\u507f\u503c\u63a5\u8fd1\u65f6\uff0c HcRed1 \u53d1\u5c04\u7684\u8367\u5149\u4fe1\u53f7\u6bd4 EYFP \u8981\u5f31\u51e0\u4e4e 100 \u500d\uff08\u56fe 6\uff08 b\uff09\uff09\u3002\u8c03\u6574 HcRed1 \u901a\u9053 PMT \u7684\u503c\u53ef\u5e73\u8861\u8367\u5149\u86cb\u767d\u7684\u53d1\u5c04\u5f3a\u5ea6\u4ee5\u514b\u670d\u8fd9\u79cd\u5dee\u5f02\uff0c\u5171\u5b9a\u4f4d\u7a0b\u5ea6\u660e\u663e\u589e\u52a0\uff08\u56fe 6\uff08 e\uff09\uff09\u3002\u56fe 6\uff08 c\uff09\u663e\u793a\u51e0\u4e2a\u53e0\u52a0\u901a\u9053\u91cd\u5408\u4e0d\u597d\uff0c\u56fe\u4e2d\u663e\u793a\u7684\u662f\u5854\u5c14\u7f8a\u5375\u5de2\u7ec6\u80de\uff0c\u7528 AlexaFluor488 \u8026\u5408\u9b3c\u7b14\u73af\u80bd\u67d3\u8272\uff08\u76ee\u6807\u5bf9\u8c61\u662f\u7eff\u8272\u4e1d\u72b6\u808c\u52a8\u86cb\u767d\uff09\uff0c\u7528 Alexa Fluor568\u4e8c\u6b21\u6297\u4f53\u67d3\u8272\uff0c\u76ee\u6807\u5bf9\u8c61\u662f\u7c98\u7740\u6591\u86cb\u767d\u6297\u4f53\uff08\u7c98\u7740\u6591\uff09\u3002\u808c\u52a8\u86cb\u767d\u4e1d\u672b\u7aef\u5e94\u8be5\u4e0e\u6837\u54c1\u4e2d\u7c98\u7740\u86cb\u767d\u5171\u5b9a\u4f4d\uff0c\u4f46\u5728\u56fe 6\uff08 c\uff09\u4e2d\uff0c\u4ed6\u4eec\u6ca1\u91cd\u5408\u597d\uff0c\u6b63\u5982\u5728\u56fe\u50cf\u53f3\u4e0b\u89d2\u7528\u7ea2\u8272\u548c\u7eff\u8272\u8367\u5149\u56e2\u6807\u8bb0\u7684\u5fae\u7403\u91cd\u5408\u4e0d\u597d\u4e00\u6837\u3002\u91c7\u96c6\u5b8c\u56fe\u50cf\u540e\uff0c\u8fdb\u884c\u56fe\u50cf\u5904\u7406\u53ef\u5c06\u901a\u9053\u5bf9\u9f50\uff0c\u6062\u590d\u56fe\u50cf\u7684\u4f4d\u7f6e\uff08\u4e5f\u5c31\u662f\u7403\u7684\u4f4d\u7f6e\uff09\uff0c\u4e3a\u5171\u5b9a\u4f4d\u5206\u6790\u63d0\u4f9b\u4e86\u5f88\u597d\u7684\u6837\u672c\u3002<\/p>\n<p style=\"text-align: left;\"><span style=\"color: #ff0000;\"><strong> \u7ed3\u8bba<\/strong><\/span><\/p>\n<p style=\"text-align: left;\">\u5f53\u7814\u7a76\u8367\u5149\u56e2\u5171\u5b9a\u4f4d\u65f6\uff0c\u8981\u8003\u8651\u7684\u6700\u91cd\u8981\u7684\u4e00\u70b9\u662f\uff1a\u786e\u4fdd\u6837\u54c1\u6807\u8bb0\u7684\u8d28\u91cf\u6700\u597d\u3002\u6297\u4f53\u548c\u5408\u6210\u8367\u5149\u56e2\u90fd\u5e94\u8fdb\u884c\u7279\u5f02\u6027\u7684\u5206\u6790\u548c\u9009\u62e9\uff0c\u80cc\u666f\u8981\u4f4e\u3001\u6ca1\u6709\u4ea4\u53c9\u6d3b\u6027\u3002\u4e3a\u4e86\u964d\u4f4e\u4e32\u8272\u5f71\u54cd\uff0c\u53d1\u5c04\u5149\u8c31\u5206\u5f97\u6bd4\u8f83\u5f00\u7684\u8367\u5149\u63a2\u9488\u7ec4\u5408\u6700\u9002\u5408\u505a\u5171\u5b9a\u4f4d\u5b9e\u9a8c\u3002\u8981\u505a\u5408\u9002\u7684 control \u5b9e\u9a8c\uff0c\u5305\u62ec\u7528\u6bcf\u4e2a\u63a2\u9488\u5355\u72ec\u6807\u8bb0\u6837\u54c1\u53ca\u672a\u6807\u8bb0\u7684\u53ea\u6709\u81ea\u53d1\u8367\u5149\u7684\u6837\u54c1\uff0c\u90fd\u5e94\u8be5\u5236\u5907\uff0c\u800c\u4e14\u5728\u771f\u6b63\u7684\u5b9e\u9a8c\u6761\u4ef6\u4e0b\u7ec6\u81f4\u5730\u89c2\u5bdf\u4e32\u8272\u3002\u6837\u54c1\u5236\u5907\u597d\u540e\uff0c\u4eea\u5668\u548c\u8f6f\u4ef6\u7684\u53c2\u6570\u90fd\u8981\u5173\u6ce8\u3002\u6709\u4e00\u70b9\u5f88\u91cd\u8981\uff0c\u8981\u8bb0\u4f4f\uff1a\u6837\u54c1\u5236\u5907\u5f97\u4e0d\u597d\u5e76\u4e0d\u80fd\u901a\u8fc7\u6570\u5b57\u56fe\u50cf\u5904\u7406\u6280\u672f\u514b\u670d\u3002\u5728\u5927\u591a\u6570\u60c5\u51b5\u4e0b\uff0c\u4f18\u5316\u67d3\u8272\u6761\u4ef6\u4f1a\u8282\u7701\u5927\u91cf\u7684\u65f6\u95f4\uff0c\u6700\u7ec8\u4ea7\u751f\u8f83\u597d\u7684\u7ed3\u679c\u3002<\/p>\n<p style=\"text-align: left;\">\u8fdb\u884c\u5171\u5b9a\u4f4d\u5206\u6790\u8fd8\u53d6\u51b3\u4e8e\u4eea\u5668\u7684\u914d\u7f6e\u8981\u6c42\uff0c\u9700\u8981\u6700\u9ad8\u8d28\u91cf\u7684\u6d88\u8272\u5dee\u663e\u5fae\u955c\u7269\u955c\uff0c\u8fd9\u5bf9\u4e8e\u964d\u4f4e\u8272\u5dee\u662f\u5f88\u5fc5\u8981\u7684\uff0c\u8272\u5dee\u4f1a\u5f71\u54cd\u5171\u5b9a\u4f4d\u7684\u5b9a\u91cf\u8ba1\u7b97\u3002\u8bb8\u591a\u751f\u4ea7\u4ea7\u5546\u5f15\u8fdb\u4e86\u5e73\u573a\u590d\u6d88\u8272\u5dee\u7269\u955c\uff0c\u8fd9\u79cd\u7269\u955c\u5bf9\u8272\u5dee\u5728\u6574\u4e2a\u53ef\u89c1\u5149\u8303\u56f4\u5185\uff08 400-700nm\uff09\u8fdb\u884c\u6821\u6b63\uff0c\u53ef\u660e\u663e\u6709\u52a9\u4e8e\u8367\u5149\u663e\u5fae\u955c\u7684\u5b9a\u91cf\u7814\u7a76\u3002\u6240\u6709\u7684\u8367\u5149\u53d1\u5c04\u6ee4\u8272\u7247\u90fd\u5e94\u8be5\u7528\u4e0e\u8367\u5149\u56e2\u53d1\u5c04\u5149\u8c31\u5339\u914d\u7684\u5e26\u901a\u900f\u5c04\u6765\u4f18\u5316\u3002\u8fd9\u662f\u63a7\u5236\u6837\u54c1\u4e2d\u5176\u4ed6\u8367\u5149\u56e2\u4e32\u8272\u6700\u91cd\u8981\u7684\u8003\u8651\u4e4b\u4e00\u3002\u5982\u679c\u5728 control \u6837\u54c1\u4e2d\u68c0\u6d4b\u5230\u4e32\u8272\uff08\u7528\u5355\u4e00\u63a2\u9488\u67d3\u8272\u6216\u672a\u67d3\u8272\u53ea\u6709\u81ea\u53d1\u8367\u5149\uff09\uff0c\u7528\u88c5\u6709 AOTF\uff08 acousto-optic tunable filter\uff09\u7684\u663e\u5fae\u955c\u8fdb\u884c\u591a\u901a\u9053\u5e8f\u5217\u626b\u63cf\u4f1a\u4ea7\u751f\u6700\u4f18\u7684\u7ed3\u679c\u3002<\/p>\n<p style=\"text-align: left;\"><span style=\"color: #ff0000;\"><strong> \u53c2\u8003\u6587\u732e<\/strong><\/span><\/p>\n<p style=\"text-align: left;\">1. Olympus Microcopy resource center<\/p>\n<p style=\"text-align: left;\">2. HandBook of Biological Confocal Microscopy (THIRD EDITION) James B.Pawley<\/p>\n<p style=\"text-align: left;\"><strong><a href=\"http:\/\/www.ceshigu.com\/\" target=\"_blank\" rel=\"noopener noreferrer\">\u6d4b\u8bd5\u8c37<\/a><\/strong>\u662f\u6750\u6599\u4eba\u65d7\u4e0b\u4e13\u6ce8\u6750\u6599\u5206\u6790\u6d4b\u8bd5\u5e73\u53f0\uff0c<strong>\u63d0\u4f9b\u5305\u62ec\u529b\u5b66\u3001\u7269\u7406\u3001\u5316\u5b66\u3001\u7ed3\u6784\u5206\u6790\u7b49\u5206\u6790\u6d4b\u8bd5\u53ca\u5176\u76f8\u5173\u6570\u636e\u5206\u6790<\/strong>\uff0c<strong>\u63d0\u4f9b\u8bba\u6587\u6da6\u8272\u3001\u63d2\u56fe\u7ed8\u5236\u3001\u6750\u6599\u8ba1\u7b97\uff0c\u4e13\u5229\u64b0\u5199\u7b49\u670d\u52a1<\/strong>\u3002\u540c\u65f6\uff0c\u6b22\u8fce\u6709\u4e00\u6280\u4e4b\u957f\u7684\u6750\u6599\u4eba\u5165\u9a7b\u6d4b\u8bd5\u8c37\uff0c\u5411\u5e7f\u5927\u4f1a\u5458\u63d0\u4f9b\u670d\u52a1\uff0c\u626b\u7801\u76f4\u8fbe\u6d4b\u8bd5\u8c37\u670d\u52a1\u4e13\u533a\uff1a<\/p>\n<p style=\"text-align: left;\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-77943 aligncenter\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/1491013294-1-300x300.png\" alt=\"\" width=\"300\" height=\"300\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/1491013294-1-300x300.png 300w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/1491013294-1-150x150.png 150w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/04\/1491013294-1.png 400w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/p>\n<p style=\"text-align: left;\">\u6750\u6599\u725b\u7f16\u8f91\u6574\u7406\u3002<\/p>\n<p style=\"text-align: left;\"><a href=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/05\/\u6d4b\u8bd5\u8c37-\u5e7f\u544a.png\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-85775\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/05\/\u6d4b\u8bd5\u8c37-\u5e7f\u544a.png\" alt=\"\" width=\"740\" height=\"231\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/05\/\u6d4b\u8bd5\u8c37-\u5e7f\u544a.png 740w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/05\/\u6d4b\u8bd5\u8c37-\u5e7f\u544a-300x94.png 300w\" sizes=\"(max-width: 740px) 100vw, 740px\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5728\u591a\u6807\u8367\u5149\u6837\u54c1\u56fe\u50cf\u4e2d\uff0c\u56e0\u4e24\u4e2a\u6216\u591a\u4e2a\u8367\u5149\u56e2\u5728\u663e\u5fae\u7ed3\u6784\u4e2d\u8ddd\u79bb\u5f88\u8fd1\uff0c\u7ecf\u5e38\u4f1a\u6709\u53d1\u5c04\u4fe1\u53f7\u53e0\u52a0\uff0c\u8fd9\u79cd\u6548\u5e94\u5c31\u79f0\u4e3a\u5171\u5b9a\u4f4d\u3002\u76ee\u524d\uff0c\u9ad8\u7279\u5f02\u6027\u5408\u6210\u8367\u5149\u56e2\u548c\u7ecf\u5178\u514d\u75ab\u8367\u5149\u6280\u672f\u7684\u5e94\u7528\u3001\u7cbe\u5bc6&#8230;<\/p>\n","protected":false},"author":30,"featured_media":77484,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"class_list":["post-77473","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-ganhuo"],"_links":{"self":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/77473"}],"collection":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/users\/30"}],"replies":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=77473"}],"version-history":[{"count":15,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/77473\/revisions"}],"predecessor-version":[{"id":85844,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/77473\/revisions\/85844"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/media\/77484"}],"wp:attachment":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=77473"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=77473"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=77473"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}