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1.\u7b80\u4ecb<\/strong><\/p>\n

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2.\u523a\u6fc0\u54cd\u5e94DNA\u6c34\u51dd\u80f6\u7684\u5236\u5907<\/strong><\/p>\n

\u4e24\u79cd\u901a\u7528\u65b9\u6cd5\u7528\u4e8e\u7ec4\u88c5\u523a\u6fc0\u54cd\u5e94\u6027DNA\u6c34\u51dd\u80f6\u3002\u7b2c\u4e00\u79cd\u7b56\u7565\u662f\u901a\u8fc7\u591a\u9f7fDNA\u5355\u5143\u4ea4\u8054\u5f62\u6210\u5168DNA\u6c34\u51dd\u80f6\uff0c\u53ef\u4ee5\u5305\u62ec\u5728\u4ea4\u8054\u7f51\u7edc\u523a\u6fc0\u54cd\u5e94\u6027\u7ec4\u5206\u4e2d\u3002\u5c3d\u7ba1\u5df2\u7ecf\u62a5\u9053\u4e86\u5168DNA\u6c34\u51dd\u80f6\u7684\u4e00\u4e9b\u5e94\u7528\uff0c\u4f8b\u5982\uff0c\u6c34\u51dd\u80f6\u4e9a\u5355\u4f4d\u901a\u8fc7\u8367\u5149\u6838\u9178\u7a33\u5b9a\u7684Ag+<\/sup>\u7eb3\u7c73\u7c07\u7684\u4ea4\u8054\uff0c\u4f46\u662f\u7531\u5168DNA\u4f5c\u4e3a\u529f\u80fd\u6750\u6599\u7ec4\u6210\u7684\u523a\u6fc0\u54cd\u5e94\u6c34\u51dd\u80f6\u7684\u6548\u76ca\u7531\u4e8e\u6838\u9178\u6210\u5206\u7684\u6210\u672c\u800c\u53d7\u5230\u9650\u5236\u3002\u89e3\u51b3\u4e0e\u5168DNA\u523a\u6fc0\u54cd\u5e94\u6838\u9178\u57fa\u6c34\u51dd\u80f6\u76f8\u5173\u4e0d\u8db3\u7684\u7b2c\u4e8c\u79cd\u65b9\u6cd5\u4e3b\u8981\u662f\u5c06\u529f\u80fd\u6027\u6838\u9178\u4e0e\u805a\u5408\u7269\u94fe\u5982\u805a\u4e19\u70ef\u9170\u80fa\u76f8\u8fde\u63a5\uff0c\u5982\u56fe1\u6240\u793a\u3002\u6839\u636e\u8fd9\u79cd\u65b9\u6cd5\uff0c\u5728\u5408\u9002\u89e6\u53d1\u5242\u7684\u5b58\u5728\u4e0b\uff0c\u4ea4\u8054\u529f\u80fd\u6027\u6838\u9178\u4fee\u9970\u7684\u805a\u5408\u7269\u94fe\u5f62\u6210\u523a\u6fc0\u54cd\u5e94\u6c34\u51dd\u80f6\u3002\u6c34\u51dd\u80f6\u7cfb\u7edf\u5728\u53d7\u5230\u53cd\u5411\u89e6\u53d1\u65f6\u53c8\u4f1a\u4f7f\u5f97\u6838\u9178\u4ea4\u8054\u5355\u5143\u89e3\u79bb\u5f97\u5230\u805a\u5408\u7269\u6eb6\u6db2\u3002\u56fe1\u4e3b\u8981\u5c55\u793a\u4e86\u523a\u6fc0\u54cd\u5e94\u6027\u4e19\u70ef\u9170\u80fa-DNA\u6c34\u51dd\u80f6\u7684\u51e0\u4e2a\u4f8b\u5b50\u3002\u56fe1A\u6240\u793a\uff0c\u5728\u7b2c\u4e00\u4e2a\u4f8b\u5b50\u4e2d\uff0c\u4e19\u70ef\u9170\u80fa\u94fe\u7528\u4e19\u70ef\u9178\u6838\u9178\u4fee\u9970\uff0c\u663e\u793a\u51fa\u81ea\u8eab\u4e92\u8865\u6027\u548c\u5185\u90e8C-C\u4e0d\u5339\u914d\u3002\u5c3d\u7ba1\u81ea\u4e92\u8865\u78b1\u57fa\u7684\u6570\u91cf\u4e0d\u8db3\u4ee5\u4ea7\u751f\u7a33\u5b9a\u7684\u4ea4\u8054\u6c34\u51dd\u80f6\u7ed3\u6784\uff0c\u4f46\u662f\u901a\u8fc7\u6dfb\u52a0Ag+<\/sup>\u5f62\u6210C-Ag+<\/sup>-C\u6865\uff0c\u534f\u540c\u7a33\u5b9a\u4e86\u4ea4\u8054\u5355\u5143\u3002\u5f62\u6210\u7684\u6c34\u51dd\u80f6\u8868\u73b0\u51fa\u9ad8\u50a8\u80fd\u6a21\u91cf\u548c\u4f4e\u635f\u8017\u6a21\u91cf\u3002\u5728\u6c34\u51dd\u80f6\u4e2d\u52a0\u5165\u534a\u80f1\u80fa\u9664\u53bb\u4ea4\u8054\u5355\u5143\u7684Ag+<\/sup>\u79bb\u5b50\uff0c\u5bfc\u81f4\u6c34\u51dd\u80f6\u89e3\u79bb\u6210\u805a\u5408\u7269\u6eb6\u6db2\uff0c\u8be5\u5faa\u73af\u53ef\u901a\u8fc7\u52a0\u5165\u9002\u5f53\u7684\u89e6\u53d1\u5242\u800c\u91cd\u590d(\u56fe1B)\u3002<\/p>\n

G-\u56db\u94fe\u4f53\/18-\u51a0-6-\u919a\u523a\u6fc0\u54cd\u5e94\u7684\u53ef\u9006\u4e19\u70ef\u9170\u80fa\u6c34\u51dd\u80f6\u7cfb\u7edf\u7684\u7814\u7a76\u5448\u73b0\u5728\u56fe1C\u4e2d\u3002\u4f7f\u7528\u4e19\u70ef\u9170\u80fa\u5171\u805a\u7269\u94fe\uff0cPA<\/sub>\u548c\u5bcc\u542bG\u7684\u4e19\u70ef\u9170\u80fa\u57fa\u6838\u9178\u5b98\u80fd\u5316\u7684\u4e19\u70ef\u9170\u80fa\u94fe\u7ec4\u88c5\u5f62\u6210\u53ef\u8f6c\u6362\u7684\u6c34\u51dd\u80f6\u57fa\u8d28\u3002\u5f53K+<\/sup>\u5b58\u5728\u65f6\uff0c\u6539\u6027\u7684\u805a\u5408\u7269\u94fe\u81ea\u7ec4\u88c5\u6210K+<\/sup>\u7a33\u5b9a\u7684G\u56db\u94fe\u4f53\u4ea4\u8054\u7684\u6c34\u51dd\u80f6\u3002\u752818-\u51a0-6\u919a\u5904\u7406\u6c34\u51dd\u80f6\u6d88\u9664G-\u56db\u94fe\u4f53\u5355\u5143\u4e2d\u7684K+<\/sup>\u79bb\u5b50\uff0c\u5bfc\u81f4\u6c34\u51dd\u80f6\u89e3\u79bb\u3002<\/p>\n

\u56fe1D\u63cf\u8ff0\u4e86\u901a\u8fc7H2<\/sub>O2<\/sub>\u5b9e\u73b0ABTS2\u2212<\/sup>\u5f00\/\u5173\u53ef\u8c03\u8282\u6c27\u5316\uff0c\u5f62\u6210\u8840\u7ea2\u7d20\/K+<\/sup>\u7a33\u5b9a\u7684G-\u56db\u94fe\u4f53\u4ea4\u8054\u7684\u6c34\u51dd\u80f6(\u5f00)\u548c\u5176\u572818-\u51a0-6-\u919a\u5b58\u5728\u4e0b\u7684\u89e3\u79bb(\u5173)\u3002\u7c7b\u4f3c\u5730\uff0c\u4f7f\u7528\u5149\u5f02\u6784\u5316\u5076\u6c2e\u82ef\u5355\u5143\u5236\u5907\u53ef\u4ee5\u5f97\u5230\u5149\u54cd\u5e94\u6027DNA\u57fa\u6c34\u51dd\u80f6\uff0c\u5982\u56fe1E\u6240\u793a\u3002\u901a\u8fc7\u4f4e\u542b\u91cf\u53cc\u4e19\u70ef\u9170\u80fa\u4ea4\u8054\u5242\u4ea4\u8054\u4e19\u70ef\u9170\u80fa\u805a\u5408\u7269\u94fe\uff0cPB<\/sub>\u548cPC<\/sub>\u5f97\u5230\u4f4e\u521a\u5ea6\u7684\u4e19\u70ef\u9170\u80fa\u6c34\u51dd\u80f6\u3002\u805a\u5408\u7269PB<\/sub>\u7528\u987a\u5f0f\u5076\u6c2e\u82ef\u5355\u5143\u5b98\u80fd\u5316\u7684\u6838\u9178\u7cfb\u94fe\u4fee\u9970\uff0c\u805a\u5408\u7269\u94fePC<\/sub>\u5305\u62ec\u4e92\u8865\u7cfb\u94fe\u3002\u901a\u8fc7\u5c06\u53cd\u5f0f\u5076\u6c2e\u82ef\u5355\u5143\u9006\u5411\u5149\u5f02\u6784\u5316\u4e3a\u987a\u5f0f\u5f02\u6784\u4f53\u72b6\u6001(\u03bb= 365nm)\uff0c\u53cc\u94fe\u4f53\u6865\u8fde\u5355\u5143\u5206\u79bb\u4ea7\u751f\u4f4e\u521a\u6027\u7684\u6c34\u51dd\u80f6\uff0c\u540c\u65f6\uff0c\u7cfb\u7edf\u53ef\u4ee5\u518d\u6b21\u5728\u8f83\u9ad8\u521a\u5ea6(\u8f83\u4f4e\u4f53\u79ef)\u548c\u8f83\u4f4e\u521a\u5ea6(\u8f83\u9ad8\u4f53\u79ef)\u4e4b\u95f4\u5faa\u73af(\u56fe1F)\u3002<\/p>\n

\"\"<\/p>\n

\u56fe1 \u6c34\u51dd\u80f6\u7684\u5236\u5907\u53ca\u523a\u6fc0\u54cd\u5e94<\/strong><\/p>\n

(A) <\/strong>\u805a\u4e19\u70ef\u9170\u80fa\/\u6838\u9178\u523a\u6fc0\u54cd\u5e94\u6c34\u51dd\u80f6\u7684\u5408\u6210\uff0c\u5728Ag+<\/sup>\u79bb\u5b50\u548c\u534a\u80f1\u80fa\u4f5c\u4e3a\u89e6\u53d1\u5242\u7684\u6761\u4ef6\u4e0b\u5b9e\u73b0\u5faa\u73af\u6c34\u51dd\u80f6-\u6eb6\u6db2\u8f6c\u53d8
\n (B) <\/strong>\u00a0Ag+<\/sup>\u79bb\u5b50\/\u534a\u80f1\u80fa\u89e6\u53d1\u7684\u4e19\u70ef\u9170\u80fa\u6c34\u51dd\u80f6\u7684\u6d41\u53d8\u8868\u5f81
\n (C) <\/strong>\u50ac\u5316\u53ef\u5207\u6362\u7684\u8840\u7ea2\u7d20\/G-\u56db\u94fe\u4f53HRP-\u6a21\u62dfDNAzyme\u6c34\u51dd\u80f6\u7684\u5408\u6210
\n (D)<\/strong> \u5faa\u73afON\/OFF\u6fc0\u6d3b\u8840\u7ea2\u7d20\/G-\u56db\u94fe\u4f53\u529f\u80fd\u5316\u6c34\u51dd\u80f6\u7684\u50ac\u5316\u529f\u80fd
\n (E) <\/strong>\u5149\u8bf1\u5bfc\u7684\u53ef\u9006\u6c34\u51dd\u80f6-\u6eb6\u6db2\u7684\u8f6c\u53d8
\n (F) <\/strong>\u5149\u54cd\u5e94DNA\u57fa\u6c34\u51dd\u80f6\u4f53\u79ef\u53d8\u5316<\/p>\n

3.\u523a\u6fc0\u54cd\u5e94DNA\u6c34\u51dd\u80f6\u5e94\u7528<\/strong><\/p>\n

3.1 \u70ed\u654f\u6027DNA\u57fa\u523a\u6fc0\u54cd\u5e94\u6c34\u51dd\u80f6<\/strong><\/p>\n

\u901a\u8fc7\u4e24\u4e2a\u4fe1\u53f7\u89e6\u53d1\u57fa\u4e8e\u523a\u6fc0\u54cd\u5e94\u6838\u9178\u7684\u6c34\u51dd\u80f6\u7684\u80fd\u529b\u4f7f\u5f97\u80fd\u591f\u5728\u591a\u4e2a\u8f93\u5165\u548c\u6761\u4ef6\u4e0b\u63a7\u5236\u53ef\u9006\u7684\u6c34\u51dd\u80f6-\u6eb6\u80f6\u8f6c\u53d8\u548c\u523a\u6fc0\u6307\u5b9a\u7684\u4f53\u79ef\u8f6c\u53d8\u3002\u805a(N-\u5f02\u4e19\u57fa\u4e19\u70ef\u9170\u80fa)\uff0cpNIPAM\uff0c\u572832o<\/sup>C\u4e0b\u53ef\u4ee5\u5b9e\u73b0\u53ef\u9006\u7684\u6c34\u51dd\u80f6\u548c\u56fa\u4f53\u8fc7\u6e21\u7684\u70ed\u654f\u6027\u805a\u5408\u7269\u3002\u4e0e\u4ec5\u5177\u6709\u53ef\u9006\u7684\u51dd\u80f6\u5230\u56fa\u4f53\u8f6c\u53d8\u7684\u5171\u4ef7\u4ea4\u8054\u7684pNIPAM\u6c34\u51dd\u80f6\u76f8\u53cd\uff0cDNA\u529f\u80fd\u5316pNIPAM\u53ef\u4ee5\u5728\u6eb6\u6db2-\u6c34\u51dd\u80f6-\u56fa\u6001\u53ef\u9006\u8f6c\u53d8\u3002\u5982\u56fe2A\u6240\u793a\uff0c\u4f7f\u7528\u5bcc\u542b\u4e19\u70ef\u9170\u80fa\u80de\u5627\u5576\u7684\u7cfb\u94fe\u5b98\u80fd\u5316\u7684NIPAM\u94fe\u3002\u5728pH=7.5\u65f6\uff0c\u805a\u5408\u7269\u4ee5\u6eb6\u6db2\u72b6\u6001\u5b58\u5728\uff1b\u7cfb\u7edf\u9178\u5316\u81f3pH = 5.2\uff0c\u5bcc\u542b\u80de\u5627\u5576\u7684\u7cfb\u94fe\u6784\u578b\u6210i-\u6a21\u4f53\u5355\u5143\uff0c\u4ea4\u8054pNIPAM\u94fe\u5f62\u6210\u6c34\u51dd\u80f6\u3002\u901a\u8fc7\u4e2d\u548c\u7cfb\u7edf\u9178\u6027\uff0c\u53ef\u4f7fi-\u6a21\u4f53\u4ea4\u8054\u89e3\u79bb\uff0c\u53d8\u6210\u6eb6\u6db2\u72b6\u6001\uff0c\u5e76\u4e14\u5728pH=7.5\u548cpH=5.2\u65f6\u7cfb\u7edf\u7684\u5faa\u73af\u5904\u7406\u663e\u793a\u6eb6\u6db2\u548c\u6c34\u51dd\u80f6\u72b6\u6001\u4e4b\u95f4\u7684\u53ef\u9006\u8fc7\u6e21(\u56fe2B)\u3002\u5c06\u6c34\u51dd\u80f6\u57fa\u8d28\u52a0\u70ed\u81f345o<\/sup>C\u5c06\u6c34\u51dd\u80f6\u8f6c\u53d8\u6210\u6536\u7f29\u7684\u56fa\u4f53\uff0c\u540c\u65f6\u5c06\u56fa\u4f53\u51b7\u5374\u81f325o<\/sup>C\u5bfc\u81f4\u6c34\u51dd\u80f6\u6eb6\u80c0\u6062\u590d(\u56fe2B)\u3002\u76f8\u4f3c\u7684\u8f6c\u53d8\u901a\u8fc7Ag+<\/sup>\u79bb\u5b50\u4ea4\u8054\u6838\u9178\u529f\u80fd\u5316\u7684pNIPAM\u5171\u805a\u7269\u94fe\uff0c\u8bf1\u5bfc\u5f62\u6210\u7cfb\u94fe\u95f4\u7684C-Ag+<\/sup>-C\u6865\u8fde\u53cc\u8054\u4f53\u3002\u6240\u5f97\u6c34\u51dd\u80f6\u5728\u52a0\u70ed\u81f345o<\/sup>C\u65f6\u8f6c\u53d8\u4e3a\u56fa\u4f53\u72b6\u6001\uff0c\u5e76\u5728\u7528\u534a\u80f1\u80fa\u5904\u7406\u65f6\u89e3\u79bb\u4e3a\u6eb6\u6db2\u72b6\u6001(\u56fe2C)\u3002<\/p>\n

\"\"<\/p>\n

\u56fe2 \u70ed\u654f\u6027DNA\u57fa\u523a\u6fc0\u54cd\u5e94pNIPAM\u6c34\u51dd\u80f6\u7684\u5408\u6210<\/strong><\/p>\n

3.2 \u836f\u7269\u63a7\u91ca<\/strong><\/p>\n

\u523a\u6fc0\u54cd\u5e94\u6027DNA\u6c34\u51dd\u80f6\u7684\u8fdb\u4e00\u6b65\u5e94\u7528\u5305\u62ec\u4f7f\u7528\u529f\u80fd\u6c34\u51dd\u80f6\u4f5c\u4e3a\u4f20\u611f\u548c\u63a7\u91ca\u57fa\u8d28\u3002\u6b64\u7c7b\u7cfb\u7edf\u7684\u4ee3\u8868\u6027\u5b9e\u4f8b\u5305\u62ec\u901a\u8fc7\u805a\u5408\u7269\u94fe\u4e0e\u6838\u9178\u9002\u4f53\u6216\u91d1\u5c5e\u79bb\u5b50\u4f9d\u8d56\u6027DNA\u9176\/\u5e95\u7269\u590d\u5408\u7269\u4ea4\u8054\u800c\u4ea7\u751f\u7684\u6c34\u51dd\u80f6\uff0c\u8fd9\u4e9b\u5df2\u7ecf\u88ab\u7528\u4e8e\u611f\u6d4b\u86cb\u767d\u8d28\uff0c\u4f8b\u5982\u51dd\u8840\u9176\uff0c\u6216\u91d1\u5c5e\u79bb\u5b50\u3002\u4fe1\u53f7\u89e6\u53d1\u6c34\u51dd\u80f6\u5411\u6eb6\u6db2\u76f8\u7684\u8f6c\u53d8\u4e5f\u63d0\u4f9b\u4e86\u91ca\u653e\u6c34\u51dd\u80f6\u8d1f\u8f7d\u5206\u5b50\u7684\u901a\u7528\u624b\u6bb5\u3002\u6c34\u51dd\u80f6\u901a\u8fc7DNA\u9176\u89e6\u53d1\u5668\u7684\u5faa\u73af\u6eb6\u89e3\u7528\u4e8e\u91ca\u653e\u9176\u8d1f\u8f7d\u548c\u6fc0\u6d3b\u9176\u7ea7\u8054(\u56fe3)\u3002\u5c06\u4e09\u79cd\u9176\uff0c\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176(\u03b2-Gal)\uff0c\u8461\u8404\u7cd6\u6c27\u5316\u9176(GOx)\u548cHRP\u88c5\u8f7d\u5728\u7531\u4e09\u79cd\u4e0d\u540c\u7684\u91d1\u5c5e\u79bb\u5b50\u4f9d\u8d56\u6027DNA\u9176\/\u5e95\u7269\u4ea4\u8054\u7684\u4e09\u79cd\u4e0d\u540c\u7684\u523a\u6fc0\u6027\u4e19\u70ef\u9170\u80faDNA\u6c34\u51dd\u80f6\u4e2d(\u56fe3A)\u3002\u03b2-Gal\u56fa\u5b9a\u5728\u7531Zn2+<\/sup>\u4f9d\u8d56\u6027DNA\u9176\u7a33\u5b9a\u7684\u6c34\u51dd\u80f6\u4e2d\uff0cGOx\u88abMg2+<\/sup>\u4f9d\u8d56\u6027DNA\u9176\u56fa\u5b9a\uff0cHRP\u88abCu2+<\/sup>\u4f9d\u8d56\u6027DNA\u9176\u56fa\u5b9a\u3002\u5728\u4e73\u7cd6\u4f5c\u4e3a\u5e95\u7269\u548cABTS2-<\/sup>\u7684\u5b58\u5728\u4e0b\uff0c\u5e76\u4e14\u5728\u52a0\u5165\u6240\u6709\u4e09\u79cd\u91d1\u5c5e\u79bb\u5b50Zn2+<\/sup>\uff0cMg2+<\/sup>\u548cCu2+<\/sup>\u540e\uff0c\u4e09\u4e2a\u9176\u7ea7\u8054\u88ab\u6d3b\u5316(\u56fe3B)\u3002\u5f97\u5230\u7684\u7740\u8272ABTS–<\/sup>\u63d0\u4f9b\u751f\u7269\u50ac\u5316\u7ea7\u8054\u7684\u8bfb\u51fa\u4fe1\u53f7(\u56fe3C)\u3002<\/p>\n

\"\"<\/p>\n

\u56fe3 \u901a\u8fc7\u4e09\u79cd\u4e0d\u540c\u6c34\u51dd\u80f6\u7684\u91d1\u5c5e\u79bb\u5b50\u89e3\u79bb\u6fc0\u6d3b\u4e09\u9176\u50ac\u5316\u7ea7\u8054<\/strong><\/p>\n

3.3 \u5f62\u72b6\u8bb0\u5fc6\u7cfb\u7edf<\/strong><\/p>\n

\u523a\u6fc0\u54cd\u5e94\u6027DNA\u6c34\u51dd\u80f6\u53ef\u4ee5\u4f5c\u4e3a\u529f\u80fd\u6750\u6599\u6765\u5b9a\u5236\u5f62\u72b6\u8bb0\u5fc6\u7cfb\u7edf\u3002\u591a\u79cd\u89e6\u53d1\u5668\u53ef\u9006\u91cd\u6784\u6838\u9178\u7ed3\u6784\u4e3a\u8bbe\u8ba1\u5f62\u72b6\u8bb0\u5fc6DNA\u6c34\u51dd\u80f6\u57fa\u8d28\u63d0\u4f9b\u4e86\u4e30\u5bcc\u7684\u65b9\u6cd5\uff0c\u5982\u56fe4A\u6240\u793a\u663e\u793a\u4e86pH\u54cd\u5e94\u5f62\u72b6\u8bb0\u5fc6\u6c34\u51dd\u80f6\u7684\u5408\u6210\u3002\u5c06\u65e0\u5b9a\u5f62\u805a\u5408\u7269\u6eb6\u6db2\u5728\u4e09\u89d2\u5f62\u6a21\u5177\u4e2d\u8c03\u8282\u81f3\u9178\u6027pH(pH=5.0)\uff0c\u5f97\u5230\u901a\u8fc7\u53cc\u94fe\u4f5311\/11\u548ci-\u6a21\u4f53\u4ea4\u805410\/10\u534f\u540c\u7a33\u5b9a\u7684\u6c34\u51dd\u80f6\u3002\u8be5\u6c34\u51dd\u80f6\u901a\u8fc7\u5faa\u73afpH\u523a\u6fc0\u53ef\u4ee5\u5b9e\u73b0\u6210\u578b\u6c34\u51dd\u80f6\u548c\u65e0\u5f62\u72b6\u51c6\u6db2\u6001\u4e4b\u95f4\u7684\u53ef\u9006\u8f6c\u53d8(\u56fe4B)\u3002<\/p>\n

\u901a\u8fc7\u8fd9\u79cd\u523a\u6fc0\u89e6\u53d1\u5f62\u72b6\u8bb0\u5fc6DNA\u6c34\u51dd\u80f6\u7684\u6982\u5ff5\u6765\u53d1\u5c55\u901a\u8fc7\u5176\u4ed6\u523a\u6fc0\u89e6\u53d1\u7684\u4e0d\u540c\u5f62\u72b6\u8bb0\u5fc6\u6c34\u51dd\u80f6\uff0c\u4f8b\u5982pH\u89e6\u53d1\u7684C-G\u2022C+<\/sup>\u6216T-A\u2022T\u4e09\u91cd\u6865\uff0cK+<\/sup>\u7a33\u5b9a\u7684G-\u56db\u94fe\u4f53\/Kryptofix [2.2.2]\u6216Ag+<\/sup>\u7a33\u5b9a\u7684C-Ag+<\/sup>-C-\u6865\u63a5\/\u534a\u80f1\u80fa\u3002\u56fe4C\u4e3e\u4f8b\u8bf4\u660e\u4e86K+<\/sup>\u79bb\u5b50\/\u51a0\u919a\u89e6\u53d1\u7684\u5f62\u72b6\u8bb0\u5fc6\u6c34\u51dd\u80f6\u7684\u7ec4\u88c5\u3002\u5982\u56fe4D\uff0c\u901a\u8fc7G-\u56db\u94fe\u4f53\u5355\u5143\u4e0e\u51a0\u919a\u7684\u5206\u79bb\u8bf1\u5bfc\u5b9a\u5f62\u548c\u65e0\u5b9a\u5f62\u72b6\u6001\u7684\u5faa\u73af\u8f6c\u53d8\uff0c\u800cK+<\/sup>\u79bb\u5b50\u7684\u518d\u52a0\u6210\u4f7fG-\u56db\u94fe\u4f53\u6865\u518d\u751f\u4ece\u800c\u6062\u590d\u6210\u5f62\u6c34\u51dd\u80f6\u3002\u5f62\u72b6\u8bb0\u5fc6DNA\u6c34\u51dd\u80f6\u7684\u6982\u5ff5\u8fdb\u4e00\u6b65\u6269\u5c55\u5230\u7ec4\u88c5\u6742\u5316\u5f62\u72b6\u8bb0\u5fc6\u6c34\u51dd\u80f6\uff0c\u5141\u8bb8\u5b9a\u5411\u89e6\u53d1\uff0c\u76ee\u6807\u5728\u6742\u5316\u6c34\u51dd\u80f6\u7ed3\u6784\u5185\u7684\u53ef\u9006\u8f6c\u53d8\uff0c\u6a2a\u8de8\u5b9a\u5f62\u6c34\u51dd\u80f6\u548c\u65e0\u5b9a\u5f62\u6db2\u4f53\u76f8\u3002<\/p>\n

\"\"<\/p>\n

\u56fe4 pH\u54cd\u5e94\u5f62\u72b6\u8bb0\u5fc6\u4e19\u70ef\u9170\u80fa-DNA\u6c34\u51dd\u80f6<\/strong><\/p>\n

(A) <\/strong>pH\u54cd\u5e94\u5f62\u72b6\u8bb0\u5fc6\u4e19\u70ef\u9170\u80fa-DNA\u6c34\u51dd\u80f6\u7684\u5408\u6210
\n (B) <\/strong>\u5faa\u73afpH\u8bf1\u5bfc\u7cfb\u7edf\u5728\u4e09\u89d2\u5f62\u6c34\u51dd\u80f6\u548c\u51c6\u6db2\u6001\u65e0\u5b9a\u5f62\u6001\u95f4\u8f6c\u53d8
\n (C) <\/strong>\u901a\u8fc7G-\u56db\u94fe\u4f53\u548c\u53cc\u94fe\u6865\u63a5\u5355\u5143\u534f\u540c\u7a33\u5b9a\u7684\u4e19\u70ef\u9170\u80fa-DNA\u6c34\u51dd\u80f6\u7684\u5408\u6210\u548c\u5f62\u72b6\u8bb0\u5fc6\u6027\u8d28
\n (D) <\/strong>\u5206\u522b\u7528K+<\/sup>\u548c\u51a0\u919a\u5904\u7406\u65f6\uff0c\u6210\u5f62\u4e09\u89d2\u5f62\u6c34\u51dd\u80f6\u548c\u65e0\u5b9a\u5f62\u51c6\u6db2\u6001\u4e4b\u95f4\u5faa\u73af\u8f6c\u53d8<\/p>\n

\u4e0a\u8ff0\u8fd9\u79cd\u7ec4\u88c5\u6982\u5ff5\u5728\u56fe5\u4e2d\u901a\u8fc7\u4e09\u81c2\u7bad\u5934\u7ed3\u6784\u7684\u7ec4\u88c5\u4e3a\u4f8b\u5448\u73b0\u3002\u6240\u6709\u4e09\u4e2a\u7bad\u5934\u5305\u62ec\u81ea\u4e92\u8865\u53cc\u94feDNA\u686514\/14\u4f5c\u4e3a\u6c34\u51dd\u80f6\u6865\u8fde\u5355\u5143\uff0c\u4f7f\u5176\u6210\u4e3a\u4ece\u521a\u6027\u6c34\u51dd\u80f6\u5230\u65e0\u5b9a\u5f62\u51c6\u6db2\u6001\u7684\u4efb\u4f55\u8f6c\u53d8\u7684\u6c38\u4e45\u8bb0\u5fc6\u3002\u56fe5B\u63cf\u7ed8\u4e86\u4f7f\u7528\u94fe24\/25\u4f5c\u4e3a\u57df\u95f4\u6865\u7684\u8fb9\u7f18\u4ea4\u8054\u5c06\u4e09\u4e2a\u7bad\u5934\u7ed3\u6784\u5728\u6a21\u5177\u4e2d\u7684\u9010\u6b65\u7ec4\u88c5\u3002\u5c06\u4e09\u4e2a\u7bad\u5934\u7ed3\u6784\u65bd\u52a0\u5230\u76f8\u5e94\u7684\u71c3\u6599\/\u53cd\u71c3\u6599\u94fe\u4ee5\u53ca\u9002\u5f53\u7684pH\u4f7f\u5f97\u81c2\u5728\u6210\u5f62\u548c\u65e0\u5f62\u72b6\u6001\u4e4b\u95f4\u5faa\u73af\u53d8\u6362\uff0c\u5982\u56fe5C\u6240\u793a\u3002<\/p>\n

\"\"<\/p>\n

\u56fe5 \u6c34\u51dd\u80f6\u7ec4\u88c5\u53ca\u5f62\u6001\u8f6c\u53d8<\/strong><\/p>\n

(A,B) <\/strong>\u4e09\u4e2a\u7bad\u5934\u5f62\u72b6\u7684\u6742\u5316\u6c34\u51dd\u80f6\u7ed3\u6784\u7684\u7ec4\u88c5\u793a\u610f\u56fe
\n (C) <\/strong>\u5728\u9002\u5f53\u89e6\u53d1\u7684\u5b58\u5728\u4e0b\uff0c\u533a\u57df\u53ef\u63a7\u7684\u51dd\u80f6-\u51c6\u6db2\u4f53\u8f6c\u53d8<\/p>\n

\u57fa\u4e8eDNA\u7684\u5f62\u72b6\u8bb0\u5fc6\u6c34\u51dd\u80f6\u7684\u6982\u5ff5\u901a\u8fc7\u8bbe\u8ba1\u7531\u4e24\u4e2a\u6b63\u4ea4\u89e6\u53d1\u5668\u89e6\u53d1\u7684\u5f62\u72b6\u8bb0\u5fc6\u6c34\u51dd\u80f6\u800c\u5f97\u5230\u8fdb\u4e00\u6b65\u5f00\u53d1(\u56fe6)\u3002\u5728\u8fd9\u4e9b\u7cfb\u7edf\u4e2d\uff0c\u6210\u5f62\u7684\u6c34\u51dd\u80f6\u901a\u8fc7\u4e24\u4e2a\u534f\u540c\u6a21\u4f53\u4ea4\u8054\u3002\u4e00\u4e2a\u4ea4\u8054\u5143\u4ef6\u5305\u62ec\u81ea\u4e92\u8865\u6865\u63a5\u5668\uff0c\u5e76\u4e14\u7b2c\u4e8c\u4ea4\u8054\u5355\u5143\u901a\u8fc7\u4e24\u4e2a\u4e0d\u540c\u7684\u6b63\u4ea4\u89e6\u53d1\u5668\u5206\u79bb\u548c\u6062\u590d\u3002\u56fe6A\u63cf\u7ed8\u4e86\u7531pH\u548cAg+<\/sup>\u79bb\u5b50\u4f5c\u4e3a\u6b63\u4ea4\u89e6\u53d1\u7684\u5f62\u72b6\u8bb0\u5fc6\u6c34\u51dd\u80f6\u3002\u901a\u8fc7\u5faa\u73af\u4f7f\u7528pH\u6216Ag+<\/sup>\/\u534a\u80f1\u80fa\u4f5c\u4e3a\u6b63\u4ea4\u89e6\u53d1\uff0c\u6c34\u51dd\u80f6\u5728\u6210\u5f62\u6c34\u51dd\u80f6\u548c\u65e0\u5b9a\u5f62\u51c6\u6db2\u6001\u4e4b\u95f4\u53ef\u9006\u8f6c\u5316(\u56fe6B)\u3002\u76f8\u7c7b\u4f3c\u7684\u7cfb\u7edf\u4f7f\u7528Pb2+<\/sup>\u6216Sr2+<\/sup>\u79bb\u5b50\u4f5c\u4e3a\u6b63\u4ea4\u89e6\u53d1\u5668\u7528\u4e8e\u6784\u5efa\u57fa\u4e8e\u53cc\u94fe\u4f53(\u6c38\u4e45\u8bb0\u5fc6)\u548cG-\u56db\u94fe\u4f53\u4f5c\u4e3a\u7a33\u5b9a\u6210\u5f62\u6c34\u51dd\u80f6\u7ed3\u6784\u7684\u534f\u540c\u4ea4\u8054\u5355\u5143\u7684\u5f62\u72b6\u8bb0\u5fc6\u6c34\u51dd\u80f6(\u56fe6C,D)\u3002<\/p>\n

\"\"<\/p>\n

\u56fe6 \u4f7f\u7528\u4e24\u4e2a\u6b63\u4ea4\u89e6\u53d1\u5668\u6fc0\u6d3b\u5f62\u72b6\u8bb0\u5fc6\u6c34\u51dd\u80f6\uff0c\u4f7f\u5176\u5728\u6210\u5f62\u6c34\u51dd\u80f6\u548c\u51c6\u6db2\u6001\u4e4b\u95f4\u5faa\u73af\u548c\u53ef\u9006\u8f6c\u6362<\/strong><\/p>\n

3.4 \u523a\u6fc0\u54cd\u5e94\u6c34\u51dd\u80f6\u8584\u819c\u6d82\u5c42<\/strong><\/p>\n

\u6c34\u51dd\u80f6\u5728\u8868\u9762\u4e0a\u7684\u6c89\u79ef\uff0c\u7279\u522b\u662f\u523a\u6fc0\u54cd\u5e94\u6027\u6c34\u51dd\u80f6\uff0c\u53ef\u4ee5\u4fc3\u8fdb\u6c34\u51dd\u80f6\u88c5\u7f6e\u7684\u53d1\u5c55\u3002\u6742\u4ea4\u94fe\u5f0f\u53cd\u5e94(HCR)\u8fc7\u7a0b\u4f5c\u4e3a\u5728\u8868\u9762\u4e0a\u7ec4\u88c5\u8584\u523a\u6fc0\u54cd\u5e94\u6c34\u51dd\u80f6\u7684\u901a\u7528\u65b9\u6cd5\u800c\u88ab\u5e7f\u6cdb\u4f7f\u7528\u3002\u8be5\u53cd\u5e94\u5305\u62ec\u4f7f\u7528\u542f\u52a8\u5b50\u6838\u9178\u5f15\u53d1\u7684\u4e24\u4e2a\u53d1\u5939HA<\/sub>\u548cHB<\/sub>\u7684\u4ea4\u53c9\u5f00\u653e\uff0c\u5176\u4e2d\u542f\u52a8\u5b50\u6253\u5f00HA<\/sub>\u4ee5\u4ea7\u751f\u7528\u4e8e\u6253\u5f00HB<\/sub>\u7684\u5355\u94fetoehold\uff0c\u5e76\u4e14\u7ee7\u800c\u4ea7\u751fHB<\/sub>-\u6253\u5f00\u7684\u4ea7\u7269\u5305\u62ec\u6253\u5f00\u53d1\u5939HA<\/sub>\u7684toehold\u533a\u57df\u3002\u53d1\u5939\u7ed3\u6784\u7684\u4ea4\u53c9\u5f00\u53e3\u88ab\u7528\u4e8e\u4ea4\u8054\u6838\u9178\u5b98\u80fd\u5316\u7684\u4e19\u70ef\u9170\u80fa\u94fe\uff0c\u4ee5\u5728\u8868\u9762\u4e0a\u4ea7\u751f\u523a\u6fc0\u54cd\u5e94\u7684\u6c34\u51dd\u80f6\u819c(\u56fe7A)\u3002\u7531\u4e8e\u6865\u8fde\u6c34\u51dd\u80f6\u5305\u62ec\u6e38\u79bb\u7684\u5bcc\u542bG\u7684\u7cfb\u94fe\uff0c\u8fd9\u4e9b\u94fe\u5728K+<\/sup>\u79bb\u5b50\u7684\u5b58\u5728\u4e0b\u7ec4\u88c5\u6210G-\u56db\u94fe\u4f53\uff0c\u5176\u534f\u540c\u5730\u7a33\u5b9a\u6c34\u51dd\u80f6\uff0c\u4ea7\u751f\u521a\u6027\u7684\u6c34\u51dd\u80f6\u3002\u752818-\u51a0\u919a-6\u919a\u5904\u7406\u819c\u4ece\u57fa\u8d28\u4e2d\u9664\u53bbK+<\/sup>\u79bb\u5b50\uff0c\u5f97\u5230\u521a\u6027\u964d\u4f4e\u7684\u6c34\u51dd\u80f6\u819c\u3002\u5fae\u538b\u75d5\u5b9e\u9a8c\u9075\u5faa\u6c34\u51dd\u80f6\u819c\u5728\u521a\u6027\u72b6\u6001\u548c\u4f4e\u521a\u6027\u72b6\u6001\u4e4b\u95f4\u5faa\u73af\u8fc7\u6e21(\u56fe7B)\u3002\u8840\u7ea2\u7d20\u4e0eG-\u56db\u94fe\u4f53\u5355\u5143\u7684\u7ed3\u5408\u4ea7\u751f\u4e86\u7528\u4e8e\u8fd8\u539fH2<\/sub>O2<\/sub>\u7684\u7535\u50ac\u5316\u5355\u5143\uff0c\u56e0\u6b64\u901a\u8fc7\u5728\u7535\u6781\u8868\u9762\u4e0a\u7ec4\u88c5\u8840\u7ea2\u7d20\/G-\u56db\u94fe\u4f53\u5b98\u80fd\u5316\u7684\u6c34\u51dd\u80f6\u8584\u819c\uff0c\u4ee5\u53ca\u968f\u540e\u51a0\u919a\u523a\u6fc0\u8840\u7ea2\u7d20\/G-\u56db\u94fe\u4f53\u7684\u5206\u79bb\uff0c\u8bc1\u660e\u4e86H2<\/sub>O2<\/sub>\u7684\u5faa\u73af\u5f00\/\u5173\u7535\u50ac\u5316\u8fd8\u539f(\u56fe7C)\u3002<\/p>\n

\"\"<\/p>\n

\u56fe7 \u6c34\u51dd\u80f6\u8584\u819c\u7684\u5236\u5907\u53ca\u5e94\u7528<\/strong><\/p>\n

(A) <\/strong>\u4f7f\u7528HCR\u5728Au\u6d82\u8986\u7684\u8868\u9762\u4e0a\u7ec4\u88c5G-\u56db\u8054\u4f53\u523a\u6fc0\u54cd\u5e94\u6027\u6c34\u51dd\u80f6\u8584\u819c
\n (B) <\/strong>\u5728\u51a0\u919a\u5b58\u5728\u4e0b\uff0c\u901a\u8fc7K+<\/sup>\u7a33\u5b9a\u7684G-\u56db\u94fe\u4f53\u4ea4\u8054\u6c34\u51dd\u80f6\u4ee5\u53ca\u5176\u89e3\u79bb\u5b9e\u73b0\u6c34\u51dd\u80f6\u819c\u7684\u521a\u6027\u53ef\u63a7
\n (C)<\/strong> \u901a\u8fc7\u8840\u7ea2\u7d20\/G-\u56db\u94fe\u4f53\u523a\u6fc0\u89e6\u53d1\u63a7\u5236H2<\/sub>O2<\/sub>\u7535\u50ac\u5316\u8fd8\u539f<\/p>\n

3.5 \u6c34\u51dd\u80f6\u5fae\u80f6\u56ca<\/strong><\/p>\n

\u7528\u523a\u6fc0\u54cd\u5e94\u6c34\u51dd\u80f6\u57fa\u8d28\u6d82\u8986\u8868\u9762\u53ef\u4ee5\u5f97\u5230\u7528\u4e8e\u89e6\u53d1\u836f\u7269\u91ca\u653e\u7684\u523a\u6fc0\u54cd\u5e94\u6c34\u51dd\u80f6\u5fae\u80f6\u56ca\u3002\u5fae\u7c73\u7ea7\u539a\u5ea6\u7684\u523a\u6fc0\u54cd\u5e94\u4e19\u70ef\u9170\u80fa-DNA\u6c34\u51dd\u80f6\u819c\u7684\u6c89\u79ef\u5df2\u7ecf\u88ab\u7528\u4e8e\u5408\u6210\u57fa\u677f\u8d1f\u8f7d\u7684\u523a\u6fc0\u6027\u6c34\u51dd\u80f6\u5fae\u80f6\u56ca\uff0c\u4ee5pH\u54cd\u5e94\u6c34\u51dd\u80f6\u5fae\u80f6\u56ca\u7684\u5236\u5907\u8fdb\u884c\u4e3e\u4f8b\u8bf4\u660e(\u56fe8A)\u3002\u8d1f\u8f7dCaCO3<\/sub>\u5fae\u7c92\u57fa\u677f\u4f7f\u7528\u805a\u4e19\u70ef\u80fa\u76d0\u9178\u76d0\u548c\u805a\u4e19\u70ef\u9178\u5c42\u6d82\u8986\u3002\u6c34\u51dd\u80f6\u6d82\u5c42\u901a\u8fc7\u53d1\u5939HC<\/sub>\/HD<\/sub>\u7684\u4ea4\u53c9\u5f00\u653e\u548c\u4e92\u8865\u53cc\u94fe\u4f5336\/37\u5f62\u6210\u7684\u534f\u540c\u53cc\u94fe\u6865\u7a33\u5b9a\u3002\u94fe36\u88ab\u8bbe\u8ba1\u6210\u5305\u542b\u5728\u9178\u6027\u6761\u4ef6(pH=5.0)\u4e0b\u91cd\u6784\u6210\u80fd\u91cf\u7a33\u5b9a\u7684i-\u6a21\u4f53\u7684\u80de\u5627\u5576\u7cfb\u94fe(\u56fe8B)\u3002\u53cc\u94fe\u4f53\u5355\u514336\/37\u7684\u5206\u79bb\u964d\u4f4e\u4e86\u6c34\u51dd\u80f6\u58f3\u7684\u521a\u5ea6\uff0c\u5bfc\u81f4\u5fae\u80f6\u56ca\u58f3\u7684\u6e17\u900f\u6027\u589e\u5f3a\u4ee5\u53ca\u57fa\u8d28\u4e2d\u7269\u8d28\u7684\u91ca\u653e\u3002\u56fe8C\u63cf\u8ff0\u4e86\u5c01\u88c5\u5728\u5fae\u80f6\u56ca\u4e2d\u7684\u5316\u7597\u836f\u7269\u963f\u9709\u7d20\u5728pH = 5.0\u7684\u65f6\u95f4\u4f9d\u8d56\u6027\u91ca\u653e\u3002\u540c\u65f6\uff0c\u4f5c\u8005\u53d1\u73b0\u523a\u6fc0\u54cd\u5e94DNA\u4ea4\u8054\u7684\u4e19\u70ef\u9170\u80fa\u5fae\u80f6\u56ca\u8868\u73b0\u51fa\u51e0\u79cd\u4e0d\u540c\u7684\u6027\u8d28\uff1a(i) \u5fae\u80f6\u56ca\u7684\u58f3\u7ef4\u6301\u5176\u50a8\u5c42\u8fb9\u754c\uff0c\u5e76\u4e14\u5728\u9002\u5f53\u523a\u6fc0\u5b58\u5728\u4e0b\uff0c\u53ef\u4ee5\u5728\u521a\u6027\u548c\u51c6\u6db2\u6001\u4e4b\u95f4\u53ef\u9006\u8f6c\u6362\uff1b(ii) \u4f4e\u5206\u5b50\u91cf\u6216\u5927\u5206\u5b50\u8d1f\u8f7d(\u4f8b\u5982\u9176)\u4e0d\u4f1a\u6269\u6563\u7a7f\u8fc7\u786c\u58f3\u5230\u672c\u4f53\u6eb6\u6db2\uff0c\u800c\u662f\u81ea\u7531\u6e17\u900f\u901a\u8fc7\u51c6\u6db2\u4f53\u58f3\u3002\u56e0\u6b64\uff0c\u523a\u6fc0\u54cd\u5e94DNA\u57fa\u4e19\u70ef\u9170\u80fa\u6c34\u51dd\u80f6\u5fae\u80f6\u56ca\u662f\u5bf9\u529f\u80fd\u6027\u5fae\u8f7d\u4f53\u4f8b\u5982\u805a\u5408\u7269\u56ca\u6ce1\u6216\u5fae\u51dd\u80f6\u7684\u4e00\u4e2a\u8865\u5145\u3002\u4f46\u662f\uff0c\u4e0e\u5141\u8bb8\u4f4e\u5206\u5b50\u91cf\u5e95\u7269\u81ea\u7531\u8fdb\u51fa\u6e17\u900f\u548c\u6291\u5236\u5927\u5206\u5b50\u8d1f\u8f7d\u6269\u6563\u7684\u8fd9\u4e9b\u7cfb\u7edf\u4e0d\u540c\uff0c\u523a\u6fc0\u54cd\u5e94DNA\u57fa\u5fae\u80f6\u56ca\u8868\u73b0\u51fa\u53ef\u63a7\u5236\u6e17\u900f\u7684\u5f00\/\u5173\u3002\u8fd9\u4e9b\u7279\u5f81\u4ee3\u8868\u4e86\u57fa\u4e8eDNA\u7684\u5fae\u51dd\u80f6\u5728\u672a\u6765\u533b\u7597\u5e94\u7528\u5177\u6709\u5176\u7279\u6709\u7684\u4f18\u52bf\u3002<\/p>\n

\"\"<\/p>\n

\u56fe8 \u6c34\u51dd\u80f6\u5fae\u80f6\u56ca\u5236\u5907\u53ca\u5e94\u7528<\/strong><\/p>\n

(A)<\/strong> \u57fa\u677f\u8d1f\u8f7dpH\u54cd\u5e94\u4e19\u70ef\u9170\u80fa-DNA\u6c34\u51dd\u80f6\u5fae\u80f6\u56ca\u7684\u5408\u6210
\n (B) <\/strong>\u901a\u8fc7\u5c06\u6c34\u51dd\u80f6\u4ea4\u8054\u5143\u4ef6\u4e4b\u4e00\u91cd\u6784\u6210i-\u6a21\u4f53\u5355\u5143\uff0c\u5b9e\u73b0pH\u8bf1\u5bfc\u5fae\u80f6\u56ca\u58f3\u6d41\u52a8\u6027\u7684\u589e\u5f3a\uff0c\u8fbe\u5230\u8f7d\u4f53pH\u53ef\u5207\u6362\u91ca\u653e
\n (C) <\/strong>\u5728(a)pH=7.2\u548c(b)pH=5.0\u65f6\uff0cpH\u54cd\u5e94\u5fae\u80f6\u56ca\u4e2d\u963f\u9709\u7d20\u4fee\u9970\u7684\u8461\u805a\u7cd6\u7684\u65f6\u95f4\u4f9d\u8d56\u6027\u91ca\u653e\uff0c\u4ee5\u53ca\u4e0d\u540cpH\u503c\u5bf9\u8367\u5149\u8d1f\u8377\u91ca\u653e\u7684\u5f71\u54cd<\/p>\n

\u3010\u7ed3\u8bba\u4e0e\u5c55\u671b\u3011<\/strong><\/p>\n

\u53ef\u8f6c\u6362\u6838\u9178\u7ed3\u6784\u7684\u4e30\u5bcc\u7684\u201c\u5de5\u5177\u7bb1\u201d\u63d0\u4f9b\u4e86\u5927\u91cf\u624b\u6bb5\u6765\u6784\u5efa\u523a\u6fc0\u54cd\u5e94\u6c34\u51dd\u80f6\uff0c\u901a\u8fc7\u53ef\u9006\u6c34\u51dd\u80f6-\u6eb6\u6db2\u76f8\u7684\u8f6c\u6362\u6765\u7ec4\u88c5\u5f62\u72b6\u8bb0\u5fc6\u6c34\u51dd\u80f6\uff0c\u5e76\u8bbe\u8ba1\u5177\u6709\u53ef\u63a7\u521a\u6027\u7684\u6c34\u51dd\u80f6\u3002\u8fd9\u79cd\u523a\u6fc0\u54cd\u5e94DNA\u57fa\u6c34\u51dd\u80f6\u53ef\u4ee5\u4f5c\u4e3a\u529f\u80fd\u57fa\u8d28\u5728\u53ef\u63a7\u836f\u7269\u8f93\u9001\u3001\u4f20\u611f\u548c\u4fe1\u606f\u50a8\u5b58\u7b49\u65b9\u9762\u5177\u6709\u4e0d\u540c\u5e94\u7528\u3002\u5c3d\u7ba1\u5728\u7814\u7a76\u57fa\u4e8eDNA\u7684\u523a\u6fc0\u54cd\u5e94\u6c34\u51dd\u80f6\u65b9\u9762\u53d6\u5f97\u4e86\u5927\u91cf\u8fdb\u5c55\uff0c\u4f46\u4ecd\u7136\u6709\u8bb8\u591a\u6311\u6218\u4e9f\u5f85\u89e3\u51b3\u3002\u4f8b\u5982\uff0c\u8bbe\u8ba1\u5149\u6216\u6c27\u5316\u8fd8\u539f\u53cd\u5e94\u89e6\u53d1\u7684\u6027\u80fd\u53ef\u63a7\u6c34\u51dd\u80f6\u7528\u4e8e\u65b0\u7684\u7535\u50ac\u5316\u6216\u5149\u50ac\u5316\u6750\u6599\u3002\u6b64\u5916\uff0c\u7eb3\u7c73\u6750\u6599(\u91d1\u5c5e\u6216\u534a\u5bfc\u4f53\u7eb3\u7c73\u7c92\u5b50\u6216\u7eb3\u7c73\u68d2)\u6216\u78b3\u6750\u6599(\u78b3\u7eb3\u7c73\u7ba1\/\u77f3\u58a8\u70ef)\u4e0e\u523a\u6fc0\u54cd\u5e94\u6027\u6c34\u51dd\u80f6\u7684\u7ed3\u5408\u53ef\u5236\u5907\u5f97\u5230\u521a\u6027\u53ef\u63a7\u7684\u6742\u5316\u57fa\u8d28\uff0c\u4f8b\u5982\u9650\u5236\u7ec6\u80de\u751f\u957f\u7684\u754c\u9762\u3002\u53e6\u5916\uff0c\u5be1\u6838\u82f7\u9178\u901a\u8fc7\u78b1\u57fa\u5bf9\u4e92\u8865\uff0c\u91d1\u5c5e\u79bb\u5b50\u6216pH\u9a71\u52a8\u6865\u63a5\u5f62\u6210\u53cc\u94fe\u4f53\u6216\u4e09\u94fe\u4f53\u7684\u56fa\u6709\u6027\u8d28\u63d0\u4f9b\u4e86\u5f00\u53d1\u81ea\u6108\u5408\u6c34\u51dd\u80f6\u7684\u624b\u6bb5\u3002\u6700\u540e\uff0c\u7528\u4e8e\u53ef\u63a7\u91ca\u653e(\u4f8b\u5982\u836f\u7269\uff0c\u9999\u6599\uff0c\u98df\u54c1\u6dfb\u52a0\u5242)\u7684\u523a\u6fc0\u54cd\u5e94\u6027\u6c34\u51dd\u80f6\u5fae\u80f6\u56ca\u7684\u5e7f\u6cdb\u5e94\u7528\u4e5f\u662f\u91cd\u8981\u7684\u53d1\u5c55\u65b9\u5411\u3002<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1a<\/strong>Stimuli-Responsive DNA-Based Hydrogels: From Basic Principles to Applications<\/a>(Acc. Chem. Res.,2017, DOI: 10.1021\/acs.accounts.6b00542)<\/p>\n

\u672c\u6587\u7531\u6750\u6599\u4eba\u9ad8\u5206\u5b50\u6750\u6599\u7ec4watermelon\u4f9b\u7a3f\uff0c\u6750\u6599\u725b\u6574\u7406\u7f16\u8f91\u3002<\/p>\n

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