{"id":63914,"date":"2017-02-07T14:00:50","date_gmt":"2017-02-07T06:00:50","guid":{"rendered":"\/\/m.iemloyee.com\/?p=63914"},"modified":"2017-02-06T12:56:32","modified_gmt":"2017-02-06T04:56:32","slug":"adv-mater-%ef%bc%9a%e7%94%9f%e7%89%a9%e7%bb%93%e6%9e%84%e7%9a%84%e6%82%ac%e6%b5%ae%e5%88%b6%e9%80%a0","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=63914","title":{"rendered":"Adv. Mater.\uff1a\u751f\u7269\u7ed3\u6784\u7684\u60ac\u6d6e\u5236\u9020"},"content":{"rendered":"

\u3010\u5f15\u8a00\u3011<\/strong><\/span><\/p>\n

\u957f\u4e45\u4ee5\u6765\uff0c\u7814\u7a76\u4eba\u5458\u8bd5\u56fe\u4f7f\u7528\u4e0e\u7ec6\u80de\u5916\u57fa\u8d28(ECM)\u5177\u6709\u7ed3\u6784\u76f8\u4f3c\u6027\u7684\u5206\u79bb\u7ec6\u80de\u548c\u805a\u5408\u7269\u6c34\u51dd\u80f6\u7684\u7ec4\u5408\u6765\u91cd\u73b0\u7ec4\u7ec7\u7ed3\u6784\u3002\u73b0\u6709\u7684\u51dd\u80f6\u6d47\u94f8\u65b9\u6cd5\u53ea\u80fd\u5b9e\u73b0\u5b8f\u89c2\u51e0\u4f55\u63a7\u5236\u3002\u52a0\u5c42\u5236\u9020(ALM)\u53ef\u521b\u9020\u51fa\u66f4\u590d\u6742\u7684\u7ed3\u6784\uff0c\u5b9e\u73b0\u5bf9\u6574\u4e2a\u7ed3\u6784\u4e2d\u7684\u7ec6\u80de\u548c\u5176\u5b83\u91cd\u8981\u7ec4\u5206\u5206\u5e03\u7684\u63a7\u5236\u3002\u4f46\u8f6f\u8d28\u6750\u6599\u7684ALM\u8868\u73b0\u51fa\u76f8\u5bf9\u4f4e\u7684\u590d\u6742\u6027\u3002\u4f7f\u7528\u60ac\u6d6e\u4ecb\u8d28\u7684\u52a0\u5c42\u5236\u9020\uff0c\u53ef\u5f97\u5230\u590d\u6742\u7684\u7ed3\u6784\uff0c\u4f46\u65e0\u6cd5\u5171\u6c89\u79ef\u591a\u79cd\u7ec6\u80de\u7c7b\u578b\uff0c\u4e14\u7531\u4e8e\u60ac\u6d6e\u4ecb\u8d28\u96be\u4ee5\u4ece\u6210\u54c1\u90e8\u4ef6\u4e2d\u5b8c\u5168\u53bb\u9664\uff0c\u5c06\u4e0d\u9002\u4e8e\u4e34\u5e8a\u4f7f\u7528\u3002<\/p>\n

\u60ac\u6d6e\u5236\u9020\u6cd5\u53ef\u5236\u5907\u5177\u6709\u4e25\u683c\u5f62\u6001\u5b9a\u4e49\u3001\u673a\u68b0\u6027\u80fd\u548c\u5316\u5b66\u6027\u80fd\u7684\u590d\u6742\u8f6f\u7ed3\u6784\u3002\u51dd\u80f6\u9897\u7c92\u4e4b\u95f4\u7684\u5f3a\u8868\u9762\u76f8\u4e92\u4f5c\u7528\u5728\u5176\u7d27\u5bc6\u63a5\u89e6\u65f6\u5f62\u6210\u77ed\u7a0b\u7c98\u8fde\uff0c\u5bfc\u81f4\u7cca\u72b6\u6750\u6599\u53d8\u7a20\u3002\u9897\u7c92\u4e4b\u95f4\u5f62\u6210\u7684\u76f8\u4e92\u4f5c\u7528\u5141\u8bb8\u9897\u7c92\u6750\u6599\u652f\u6491\u7c7b\u4f3c\u5bc6\u5ea6(\u6216\u66f4\u9ad8)\u7684\u7b2c\u4e8c\u76f8\u3002\u7531\u4e8e\u51dd\u80f6\u9897\u7c92\u662f\u79bb\u6563\u5b9e\u4f53\uff0c\u4e0d\u6c61\u67d3\u6240\u5236\u9020\u6837\u54c1\u7684\u8868\u9762\u3002\u4f7f\u7528\u9897\u7c92\u72b6\u51dd\u80f6\u4f5c\u4e3a\u60ac\u6d6e\u5242\uff0c\u4ee5\u7c7b\u4f3c\u4e8e\u53d1\u80b2\u4e2d\u7684\u80ce\u513f\u5728\u7f8a\u6c34\u4e2d\u60ac\u6d6e\u7684\u65b9\u5f0f\u652f\u6491\u6837\u54c1\u3002\u8fd9\u79cd\u65b9\u6cd5\u53ef\u7528\u4e8e\u751f\u4ea7\u590d\u6742\u7684\u8f7d\u6709\u7ec6\u80de\u7684\u7ed3\u6784\u3002<\/p>\n

\u3010\u6210\u679c\u7b80\u4ecb\u3011<\/span><\/strong><\/p>\n

\u8fd1\u65e5\uff0c\u82f1\u56fd\u4f2f\u660e\u7ff0\u5927\u5b66<\/span>Liam M. Grover<\/span>\u548c\u82f1\u56fd\u54c8\u5fb7\u5c14\u83f2\u65af\u5927\u5b66<\/span><\/strong>Alan M. Smith<\/strong><\/span> (\u5171\u540c\u901a\u8baf\u4f5c\u8005)<\/strong><\/span>\u7b49\u4eba\u4f7f\u7528\u5728\u5ba4\u6e29\u4e0b\u7a33\u5b9a\u7684\u81ea\u6108\u5408\u51dd\u80f6\u9897\u7c92(\u901a\u5e38\u79f0\u4e3a\u6d41\u4f53\u6216\u526a\u5207\u51dd\u80f6)\u4f5c\u4e3a\u201c\u5e8a\u4f53\u201d\uff0c\u4e3a\u6574\u4e2a\u90e8\u4ef6\u63d0\u4f9b\u652f\u6491\u3002\u901a\u8fc7\u80f6\u51dd\u6750\u6599\u5206\u6563\u5230\u652f\u6491\u6d41\u4f53\u51dd\u80f6\u9897\u7c92\u4e4b\u95f4\u7684\u95f4\u9699\u4e2d\u5f62\u6210\u6700\u7ec8\u7ed3\u6784\u3002\u5b9e\u73b0\u4e86\u76f8\u5bf9\u590d\u6742\u7684\u7ed3\u6784\u5316\uff0c\u540c\u65f6\u63d0\u4f9b\u8db3\u591f\u7684\u652f\u6491\u4ee5\u9632\u6b62\u7ed3\u6784\u5728\u5176\u81ea\u8eab\u91cd\u91cf\u4e0b\u584c\u9677\u3002\u5f62\u6210\u7684\u652f\u67b6\u7ed3\u6784\uff0c\u53ef\u901a\u8fc7\u6e29\u548c\u5730\u65bd\u52a0\u526a\u5207\u79fb\u9664\u652f\u6491\u76f8\uff0c\u540c\u65f6\u4fdd\u6301\u57f9\u517b\u7269\u4e2d\u4eba\u539f\u4ee3\u8f6f\u9aa8\u7ec6\u80de\u7fa4\u7684\u5b58\u6d3b\u529b\u3002\u60ac\u6d6e\u5236\u9020\u6cd5\u53ef\u4ee5\u5f62\u6210\u5177\u6709\u4eff\u5929\u7136\u8f6f\u9aa8\u73af\u5883\u7684\u5404\u5411\u5f02\u6027\u673a\u68b0\u6027\u80fd\u7684\u590d\u5408\u6c34\u51dd\u80f6\u7ed3\u6784\u3002\u8fd9\u4e9b\u652f\u67b6\u53ef\u4ee5\u7528\u4e8e\u751f\u4ea7\u9aa8\u8f6f\u9aa8\u585e\u4ee5\u5e94\u5bf9\u9010\u5e74\u589e\u52a0\u7684\u5168\u5c42\u8f6f\u9aa8\u7f3a\u635f\u75be\u75c5\u3002\u8be5\u6210\u679c\u4ee5\u201cSuspended Manufacture of Biological Structures<\/a><\/strong><\/span>\u201d\u4e3a\u9898\u4e8e2017\u5e742\u67081\u65e5\u53d1\u8868\u5728\u671f\u520aAdvanced Materials<\/span><\/strong>\u4e0a\u3002<\/p>\n

\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/strong><\/span><\/p>\n

\u56fe1\uff1a\u4f7f\u7528\u60ac\u6d6e\u6c89\u79ef\u65b9\u6cd5\u5236\u90203D\u8f6f\u56fa\u4f53\u7ed3\u6784\u793a\u610f\u56fe<\/span><\/strong><\/p>\n

\"\"<\/p>\n

(A) \u5728\u5bb9\u5668\u4e2d\u4ea7\u751f\u201c\u6d41\u4f53-\u51dd\u80f6\u201d\u652f\u6491\u57fa\u8d28<\/p>\n

(B) \u5c06\u7b2c\u4e8c\u76f8\u6324\u5165\u9897\u7c92\u5e8a\u4e2d<\/p>\n

(C) \u53ef\u81ea\u6108\u5408\u7684\u6d41\u4f53\u51dd\u80f6\uff0c\u5728\u4ea4\u8054\u8fc7\u7a0b\u4e2d\u652f\u6491\u51dd\u80f6\u7ed3\u6784<\/p>\n

(D) \u4e00\u65e6\u4ea4\u8054\uff0c\u53ef\u4ee5\u5c06\u7269\u4f53\u4ece\u9897\u7c92\u5e8a\u4e0a\u79fb\u9664<\/p>\n

(E) \u5236\u9020\u88c5\u8f7d\u6709\u7f9f\u57fa\u78f7\u7070\u77f3\u7eb3\u7c73\u9897\u7c92\u7684\u7b80\u5355\u87ba\u65cb\uff0c\u5e76\u4f7f\u7528micro-CT\u6210\u50cf<\/p>\n

\u56fe2\uff1a\u9aa8\u8f6f\u9aa8\u7f3a\u9677\u7684\u6a21\u62df\u7ed3\u6784\u53ca\u7ec6\u80de\u7ec4\u7ec7\u652f\u67b6\u7684\u5236\u5907<\/span><\/strong><\/p>\n

\"\"
\n\u5c06\u88c5\u8f7d\u6709\u539f\u4ee3\u4eba\u8f6f\u9aa8\u7ec6\u80de\u7684\u51b7\u51dd\u80f6\u57fa\u8d28\u901a\u8fc7\u5185\u5f84\u4e3a337\u03bcm\u7684\u76ae\u4e0b\u6ce8\u5c04\u9488\u6324\u5165\u5230\u201c\u6d41\u4f53-\u51dd\u80f6\u201d\u7ed3\u6784\u4e2d\uff0c\u7ef4\u6301\u6574\u4e2a\u7ed3\u6784\u4e2d\u7684\u7ec6\u80de\u6d3b\u529b\u3002\u9488\u5185\u5f84\u7684\u589e\u52a0\u5bfc\u81f4\u5206\u8fa8\u7387\u964d\u4f4e(\u4e2d\u5fc3\uff0c\u9876\u90e8)\uff0c\u57fa\u8d28(\u4e2d\u5fc3\uff0c\u5e95\u90e8)\u7684\u7c98\u5ea6\u964d\u4f4e\u3002\u4ece\u8fd9\u4e9b\u5b9e\u9a8c\u4e2d\u5f97\u51fa\uff0c\u4e3a\u4e86\u5236\u9020\u8f6f\u9aa8\u585e\uff0c\u8bbe\u7f6e\u6d41\u4f53\u7c98\u5ea6\u4e3a0.75Pa s(\u57281s-1<\/sup>\u7684\u526a\u5207\u901f\u7387\u4e0b)\uff0c\u9488\u76f4\u5f84\u4e3a337\u03bcm\u3002\u8be5\u8f6f\u9aa8\u585e\u7684\u8ba1\u7b97\u673a\u6a21\u578b(\u53f3\u680f)\u7531\u4e34\u5e8a\u533b\u751f\u5bf9\u94bb\u51fa\u7f3a\u9677\u7684\u7ec4\u7ec7\u8fdb\u884cCT\u626b\u63cf\u4ea7\u751f\u3002 \u6240\u5f97\u6a21\u578b\u7528\u4e8e\u5236\u9020\u5177\u6709\u5408\u9002\u7684\u8f6f\u9aa8\u548c\u8f6f\u9aa8\u4e0b\u539a\u5ea6\u7684\u8f6f\u9aa8\u585e\u4ee5\u9002\u5408\u7ec4\u7ec7\u7f3a\u9677\u3002<\/p>\n

\u56fe3\uff1a3D\u9aa8\u8f6f\u9aa8\u7f3a\u635f\u6a21\u578b\u7684\u673a\u68b0\u6027\u80fd\u3001\u751f\u7269\u6d3b\u6027\u53ca\u8868\u578b\u7814\u7a76<\/span><\/strong><\/p>\n

\"\"<\/p>\n

(A) \u5728\u7528\u5200\u7247\u5207\u5272\u5e76\u4f7f\u7528\u6d41\u53d8\u4eea\u8fdb\u884c\u673a\u68b0\u8868\u5f81\u4e4b\u524d\uff0c\u5bf9\u4f7f\u7528\u60ac\u6d6e\u5236\u9020\u6cd5\u5236\u5907\u7684\u6837\u54c1\u8fdb\u884c\u57f9\u517b<\/p>\n

(B) \u6784\u5efa\u4f53\u7684\u50a8\u80fd\u6a21\u91cf\u4ece\u7ed3\u6784\u7684\u6838\u5fc3\u201c\u9aa8\u201d\u533a\u57df(\u533a\u57dfC\u548cD)\u5230\u8f6f\u9aa8\u533a\u57df(\u533a\u57dfA\u548cB)\u5448\u73b0\u51fa\u663e\u8457\u51cf\u5c11\u7684\u8d8b\u52bf\u3002\u5728\u5929\u7136\u7ec4\u7ec7\u4e2d\uff0c\u50a8\u80fd\u6a21\u91cf\u4ece\u900f\u660e\u8f6f\u9aa8(\u533a\u57dfA)\u901a\u8fc7\u9aa8\u8f6f\u9aa8\u754c\u9762(\u533a\u57dfB)\u5230\u8f6f\u9aa8\u4e0b\u9aa8(\u533a\u57dfC\u548cD)\u9010\u6e10\u589e\u52a0\u3002\u8be5\u529b\u5b66\u6570\u636e\u53cd\u6620\u4e86\u5728\u5929\u7136\u7ec4\u7ec7\u4e2d\u89c2\u5bdf\u5230\u7684\u8d8b\u52bf\uff0c\u8fd9\u8868\u660e\uff0c\u6709\u53ef\u80fd\u5728\u9650\u5b9a\u51e0\u4f55\u5f62\u72b6\u7684\u540c\u65f6\u786e\u5b9a\u6240\u5f97\u7ed3\u6784\u8868\u73b0\u51fa\u7684\u673a\u68b0\u6027\u80fd \u00a0\u00a0[\u6ce8\uff1a<\/strong>\u5c06\u6784\u5efa\u4f53\u5207\u6210A\u3001B\u3001C\u548cD\u56db\u4e2a\u533a\u57df\uff0c\u5e76\u5728\u6bcf\u4e2a\u90e8\u5206\u4e0a\u8fdb\u884c\u5e94\u529b\u626b\u63cf\u4ee5\u786e\u5b9a\u673a\u68b0\u5f3a\u5ea6\u548c\u5f39\u6027]<\/strong><\/p>\n

(C) \u5e94\u529b\u677e\u5f1b\u6d4b\u91cf\u663e\u793a\u52a0\u5165\u7f9f\u57fa\u78f7\u7070\u77f3\u7684\u5409\u5170\u7cd6\u80f6(GG + HAp)\u4f1a\u6bd4\u5355\u4e00\u7684\u5409\u5170\u7cd6\u80f6(GG)\u8868\u73b0\u51fa\u66f4\u5feb\u7684\u5e94\u529b\u677e\u5f1b\u54cd\u5e94\u00a0 \u00a0[\u6ce8\uff1a<\/strong>\u66f4\u5feb\u5730\u5e94\u529b\u677e\u5f1b\u54cd\u5e94\u4f1a\u66f4\u5927\u7a0b\u5ea6\u7684\u4fc3\u8fdb\u77ff\u5316]<\/strong><\/p>\n

(D) \u6784\u5efa\u4f53\u5728\u4eba\u7ec4\u7ec7\u7f3a\u9677\u5185\u57f9\u517b4\u5468\u540e\u9664\u53bb\uff0c\u56de\u6536\u8f6f\u9aa8(CH)\u3001\u9aa8\u754c\u9762(IF)\u548c\u9aa8\u533a\u57df(OB)\u5185\u7684\u7ec6\u80de\u7528\u4e8e\u5206\u79bbRNA\uff0c\u901a\u8fc7\u5b9a\u65f6\u5b9a\u91cfPCR(qRT-PCR)\u5206\u6790mRNA\u3002\u652f\u67b6\u8f6f\u9aa8\u90e8\u5206\u7684\u7ec6\u80de\u8868\u8fbe\u51fa\u6700\u9ad8\u6c34\u5e73\u7684\u80f6\u539fII\u548c\u805a\u96c6\u86cb\u767d\u805a\u7cd6(ACAN)\uff0c\u9aa8\u533a\u57df\u8868\u8fbe\u51fa\u66f4\u591a\u7684\u80f6\u539fIA1\u3002\u8fd9\u8868\u660e\u6c89\u79ef\u5230\u79bb\u6563\u533a\u57df\u7684\u7ec6\u80de\u7ef4\u6301\u751f\u5b58\u529b\u4e14\u7ef4\u6301\u5176\u8868\u578b<\/p>\n

(E) \u8367\u5149\u514d\u75ab\u7ec4\u7ec7\u5316\u5b66(IHC) (DAPI(\u82ef\u57fa\u5432\u54da)=\u84dd\u8272\uff0c\u805a\u96c6\u86cb\u767d\u805a\u7cd6=\u7eff\u8272)\u663e\u793a\u51fa\uff0c\u5728\u6784\u5efa\u4f53\u7684\u8f6f\u9aa8\u533a\u57df\u4e2d\u4ea7\u751f\u4e86\u805a\u96c6\u86cb\u767d\u805a\u7cd6 \u00a0[\u6ce8\uff1a<\/strong>\u8f6f\u9aa8\u5f62\u6210\u7684\u6807\u5fd7\u7269]<\/strong><\/p>\n

\u3010\u5c0f\u7ed3\u3011<\/span><\/strong><\/p>\n

\u901a\u8fc7\u5c06\u51dd\u80f6\u805a\u5408\u7269\u6324\u5165\u57fa\u4e8e\u9897\u7c92\u7684\u652f\u6491\u57fa\u8d28\u6765\u5236\u9020\u76f8\u5bf9\u590d\u6742\u7684\u8f6f\u56fa\u4f53\u7ed3\u6784\u3002\u53ef\u5b9e\u73b0\u8f6f\u56fa\u4f53\u6750\u6599\u7684\u7ed3\u6784\u5316\uff0c\u4f7f\u5b83\u4eec\u5728\u5fae\u5c3a\u5ea6\u4e0a\u8868\u73b0\u51fa\u4e0d\u540c\u7684\u5316\u5b66\u548c\u7269\u7406\u6027\u8d28\u3002\u60ac\u6d6e\u5236\u9020\u53ef\u4ee5\u91cd\u73b0\u7531CT\u626b\u63cf\u5b9a\u4e49\u7684\u9aa8\u8f6f\u9aa8\u533a\u57df\u7684\u7ed3\u6784\u30023D\u6253\u5370\u7684\u7ed3\u6784\u5728\u56db\u5468\u57f9\u517b\u671f\u5185\u4fdd\u6301\u5176\u5f62\u6001\u548c\u673a\u68b0\u7a33\u5b9a\u6027\uff0c\u56fa\u5b9a\u5316\u7ec6\u80de\u4fdd\u6301\u5176\u8868\u578b\u3002\u7814\u7a76\u7ed3\u679c\u8868\u660e\u8fd9\u79cd\u751f\u4ea73D\u7ec4\u7ec7\u6837\u7ed3\u6784\u7684\u65b0\u65b9\u6cd5\u5728\u518d\u751f\u548c\u7814\u7a76\u590d\u6742\u7ec4\u7ec7\u7ed3\u6784\u53ca\u754c\u9762\u7b49\u9886\u57df\u5177\u6709\u5e7f\u9614\u7684\u53d1\u5c55\u524d\u666f\u3002<\/p>\n

\u6587\u732e\u94fe\u63a5<\/span><\/strong>\uff1aSuspended Manufacture of Biological Structures<\/strong><\/span><\/a> (Adv. Mater., 2017, DOI: 10.1002\/adma.201605594)<\/p>\n

\u672c\u6587\u7531\u6750\u6599\u4eba\u751f\u7269\u6750\u6599\u7ec4\u767d\u7693\u4f9b\u7a3f\uff0c\u6750\u6599\u725b\u7f16\u8f91\u6574\u7406\u3002<\/p>\n

\u53c2\u4e0e\u751f\u7269\u6750\u6599\u8bdd\u9898\u8ba8\u8bba\u6216\u4e86\u89e3\u751f\u7269\u6750\u6599\u5c0f\u7ec4\u62db\u52df\u8be6\u60c5\uff0c\u8bf7\u52a0\u5165\u6750\u6599\u4eba\u751f\u7269\u6750\u6599\u4ea4\u6d41\u7fa4(124806506)\u3002
\n\u6750\u6599\u725b\u7f51\u4e13\u6ce8\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u53ca\u884c\u4e1a\u8fdb\u5c55\uff0c\u8fd9\u91cc\u6c47\u96c6\u4e86\u5404\u5927\u9ad8\u6821\u7855\u535a\u751f\u3001\u4e00\u7ebf\u79d1\u7814\u4eba\u5458\u4ee5\u53ca\u884c\u4e1a\u4ece\u4e1a\u8005\uff0c\u5982\u679c\u60a8\u5bf9\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u8fdb\u5c55\uff0c\u89e3\u8bfb\u9ad8\u6c34\u5e73\u6587\u7ae0\u6216\u662f\u8bc4\u8ff0\u884c\u4e1a\u6709\u5174\u8da3\uff0c\u70b9\u6211\u52a0\u5165\u7f16\u8f91\u90e8<\/strong><\/span><\/a>\u3002<\/p>\n

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