{"id":63133,"date":"2017-01-31T06:30:37","date_gmt":"2017-01-30T22:30:37","guid":{"rendered":"\/\/m.iemloyee.com\/?p=63133"},"modified":"2017-01-29T10:54:32","modified_gmt":"2017-01-29T02:54:32","slug":"angew-chem-int-ed-%ef%bc%9a%e9%80%9a%e8%bf%87fret%e6%88%90%e5%83%8f%e5%ae%9e%e6%97%b6%e7%9b%91%e6%b5%8b%e7%ba%b3%e7%b1%b3%e9%a2%97%e7%b2%92%e7%9a%84%e5%bd%a2%e6%88%90","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=63133","title":{"rendered":"Angew. Chem. Int. Ed. \uff1a\u901a\u8fc7FRET\u6210\u50cf\u5b9e\u65f6\u76d1\u6d4b\u7eb3\u7c73\u9897\u7c92\u7684\u5f62\u6210"},"content":{"rendered":"

\u3010\u5f15\u8a00\u3011<\/strong><\/p>\n

FRET\uff08F\u00f6rster Resonance Energy Transfer\/Fluorescence Resonance Energy Transfer\uff09\u5373\u8367\u5149\u80fd\u91cf\u5171\u632f\u8f6c\u79fb\uff0c\u6307\u7684\u662f\u4e24\u4e2a\u751f\u8272\u56e2\u95f4\u7684\u80fd\u91cf\u5171\u632f\u8f6c\u79fb\u73b0\u8c61\u3002\u8be5\u73b0\u8c61\u5bf9\u5fae\u5c0f\u8ddd\u79bb\u7684\u53d8\u5316\u5341\u5206\u654f\u611f\u3002\u4ee5\u6b64\u4e3a\u57fa\u7840\u53d1\u5c55\u7684FRET\u6280\u672f\uff0c\u901a\u8fc7\u6d4b\u91cf\u80fd\u91cf\u8f6c\u79fb\u6548\u7387\uff0c\u53ef\u8ba1\u7b97\u51fa\u8367\u5149\u751f\u8272\u56e2\u95f4\u7684\u8ddd\u79bb\uff08\u7ea61~10nm\uff09\uff0c\u6545\u53ef\u5e38\u7528\u4e8e\u68c0\u6d4b\u751f\u7269\u5206\u5b50\u7684\u7eb3\u7c73\u7ea7\u8ddd\u79bb\u53ca\u8ddd\u79bb\u53d8\u5316\u3002<\/p>\n

\u81ea\u7ec4\u88c5\u7eb3\u7c73\u9897\u7c92\uff08Self-assembled nanoparticles\uff0c\u7b80\u79f0SANP\uff09\u5728\u751f\u7269\u533b\u836f\u65b9\u9762\u6709\u7740\u5e7f\u6cdb\u7684\u5e94\u7528\uff0c\u5176\u5728\u6210\u50cf\u4ee5\u53ca\u836f\u7269\u4f20\u8f93\u4e0a\u7684\u4f7f\u7528\u63d0\u9ad8\u4e86\u533b\u7597\u8bca\u65ad\u53ca\u6551\u6cbb\u6c34\u5e73\u3002\u800c\u4e86\u89e3\u81ea\u7ec4\u88c5\u7eb3\u7c73\u9897\u7c92\u7684\u5f62\u6210\u8fc7\u7a0b\u5bf9\u5176\u8bbe\u8ba1\u548c\u751f\u4ea7\u6709\u5341\u5206\u91cd\u8981\u7684\u610f\u4e49\u3002\u4e00\u76f4\u4ee5\u6765\uff0c\u5bf9\u81ea\u7ec4\u88c5\u7eb3\u7c73\u9897\u7c92\u7684\u5f62\u6210\u8fc7\u7a0b\u90fd\u662f\u57fa\u4e8e\u5bf9\u6700\u7ec8\u4ea7\u7269\u7684\u8868\u5f81\u3002\u7136\u800c\uff0c\u5bf9\u8be5\u8fc7\u7a0b\u7684\u5b9e\u65f6\u89c2\u5bdf\u624d\u66f4\u6709\u5229\u4e8e\u52a0\u6df1\u5bf9\u5176\u7684\u4e86\u89e3\uff0c\u4ece\u800c\u63d0\u9ad8\u7eb3\u7c73\u6750\u6599\u7684\u8bbe\u8ba1\u4e0e\u8bbe\u5907\u65b9\u6cd5\u3002<\/p>\n

\u5fae\u6d41\u63a7\u6280\u672f\u7684\u53d1\u5c55\u63d0\u4f9b\u4e86\u4e00\u4e2a\u7814\u7a76\u81ea\u7ec4\u88c5\u7eb3\u7c73\u9897\u7c92\u5f62\u6210\u7684\u7edd\u4f73\u5e73\u53f0\u3002\u5728\u5fae\u6d41\u63a7\u6280\u672f\u4e0b\uff0c\u81ea\u7ec4\u88c5\u7eb3\u7c73\u9897\u7c92\u5f62\u6210\u8fc7\u7a0b\u4ece\u968f\u65f6\u95f4\u53d8\u5316\u7684\u8fc7\u7a0b\u8f6c\u53d8\u4e3a\u7a7a\u95f4\u5206\u5e03\uff0c\u4ece\u800c\u4e3a\u6210\u50cf\u53ca\u5206\u6790\u63d0\u4f9b\u4e86\u4fbf\u5229\u3002<\/p>\n

\u3010\u6210\u679c\u7b80\u4ecb\u3011<\/strong><\/p>\n

\u5728Angewandte Chemie International Edition\u4e0a\u520a\u767b\u7684Real-Time Monitoring of Nanoparticle Formation by FRET Imaging\u4e00\u6587\u4e2d\uff0c\u897f\u5948\u5c71\u4f0a\u574e\u533b\u5b66\u9662\u7684Willem J. M. Mulder\u6559\u6388\u3001 Yiming Zhao\u535a\u58eb\uff08\u5171\u540c\u901a\u8baf\u4f5c\u8005\uff09<\/strong>\u7b49\u628a\u5fae\u6d41\u63a7\u7eb3\u7c73\u7c92\u5b50\u5408\u6210\u6cd5\u4ee5\u53caFRET\u663e\u5fae\u6210\u50cf\u6280\u672f\u7ed3\u5408\u8d77\u6765\uff0c\u5b9e\u73b0\u4e86\u7eb3\u7c73\u9897\u7c92\u7684\u81ea\u7ec4\u88c5\u8fc7\u7a0b\u7684\u5b9e\u65f6\u53ef\u89c6\u5316\u3002\u8fd9\u4e2a\u65b0\u6280\u672f\u53ef\u4ee5\u5e94\u7528\u5728\u7eb3\u7c73\u4e73\u3001\u5d4c\u6bb5\u5171\u805a\u7269\u8f7d\u836f\u80f6\u675f\u7b49\u4e0d\u540c\u7684\u7eb3\u7c73\u7cfb\u7edf\u4e2d\u3002\u8be5\u6587\u7ae0\u5c55\u793a\u4e86\u8fd9\u4e2a\u65b0\u6280\u672f\u5728\u5bf9\u5f71\u54cd\u7eb3\u7c73\u9897\u7c92\u5f62\u6210\u4e3b\u8981\u56e0\u7d20\u7684\u7814\u7a76\u4e2d\u7684\u4f18\u52bf\u3002<\/p>\n

\u6839\u636e\u8be5\u6587\u7ae0\u4f5c\u8005\u7684\u8bbe\u8ba1\uff0c\u81ea\u7ec4\u88c5\u7eb3\u7c73\u9897\u7c92\u5728\u5fae\u6d41\u63a7\u88c5\u7f6e\u4e2d\u901a\u8fc7\u8fde\u7eed\u6d41\u5f62\u6210\uff1b\u540c\u65f6\uff0cFRET\u663e\u5fae\u6210\u50cf\u6280\u672f\u4e5f\u5e94\u7528\u5728\u8be5\u88c5\u7f6e\u4e2d\u3002\u4e3a\u4e86\u5b9e\u73b0\u8fd9\u4e2a\u8bbe\u8ba1\u5e76\u6d4b\u8bd5\u5176\u666e\u9002\u6027\uff0c\u4f5c\u8005\u5728\u4e0d\u540c\u5408\u6210\u73af\u5883\u3001\u6750\u6599\u7ec4\u6210\u4e0b\u5bf9\u4e09\u79cd\u88ab\u5e7f\u6cdb\u4f7f\u7528\u7684\u81ea\u7ec4\u88c5\u7eb3\u7c73\u9897\u7c92\u7cfb\u7edf\uff1a\u7eb3\u7c73\u4e73\u3001\u805a\u5408\u7269\u80f6\u675f\u3001\u91cd\u7ec4\u7684\u9ad8\u5bc6\u5ea6\u8102\u86cb\u767d\u8fdb\u884c\u4e86\u7814\u7a76\uff1b\u5e76\u5bf9\u6bcf\u4e00\u4e2a\u7cfb\u7edf\u90fd\u5f15\u5165\u4e86\u4e0d\u540c\u7684FRET\u4f9b\u4f53-\u53d7\u4f53\u5bf9\uff0c\u5bf9\u591a\u4e2a\u5f71\u54cd\u81ea\u7ec4\u88c5\u7eb3\u7c73\u9897\u7c92\u5408\u6210\u7684\u56e0\u7d20\u8fdb\u884c\u4e86\u7814\u7a76\u3002\u8be5\u6587\u7ae0\u5c55\u793a\u4e86\u4f5c\u8005\u5bf9\u4e0a\u8ff0\u4e09\u79cd\u4e0d\u540c\u7684\u81ea\u7ec4\u88c5\u7eb3\u7c73\u9897\u7c92\u5f62\u6210\u8fc7\u7a0b\u8fdb\u884c\u5b9e\u65f6\u53ef\u89c6\u5316\u7684\u6210\u679c\uff0c\u4ece\u800c\u4ecb\u7ecd\u4e86\u901a\u8fc7FRET\u6210\u50cf\u5bf9\u7eb3\u7c73\u9897\u7c92\u7684\u5f62\u6210\u8fdb\u884c\u5b9e\u65f6\u89c2\u5bdf\u7684\u6280\u672f\u3002\u8be5\u6280\u672f\u6709\u671b\u52a0\u6df1\u4eba\u4eec\u5bf9\u5404\u79cd\u7c7b\u578b\u7eb3\u7c73\u9897\u7c92\u7684\u5408\u6210\u3001\u5f62\u6210\u673a\u5236\u4ee5\u53ca\u529f\u80fd\u7684\u4e86\u89e3\u4e0e\u7814\u7a76\u3002<\/p>\n

\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/strong><\/p>\n

\u56fe1 \u5bf9\u7eb3\u7c73\u4e73\u5f62\u6210\u8fc7\u7a0b\u7684\u5b9e\u65f6\u89c2\u6d4b<\/strong><\/p>\n

\"\"<\/a>
\n(a) FRET\u673a\u5236\u53ca\u5b9e\u9a8c\u88c5\u7f6e\u793a\u610f\u56fe\u3002<\/p>\n

(b) \u4e0d\u540cDiI\/DiO\u6d53\u5ea6\u6bd4\u4f8b\u7eb3\u7c73\u4e73\u7684\u53d1\u5c04\u5149\u8c31\uff0c\u6fc0\u53d1\u5149\u6ce2\u957f\u4e3a458nm\u3002<\/p>\n

(c) FRET\/DiO\u5cf0\u503c\u6bd4\u4e0eDiI\/DiO\u6d53\u5ea6\u6bd4\u7684\u76f8\u5173\u6027\u3002<\/p>\n

\u56fe2 \u7eb3\u7c73\u4e73\u5f62\u6210\u8fc7\u7a0b\u7684\u5171\u7126\u663e\u5fae\u56fe<\/strong><\/p>\n

\"\"<\/a>
\n(a) \u5fae\u6d41\u63a7\u88c5\u7f6e\u8154\u5185\u8d77\u59cb\u6bb5\u62cd\u6444\u56fe\uff1b\u767d\u8272\u7bad\u5934\u6240\u6307\u4e3a\u78f7\u9178\u76d0\u7f13\u51b2\u6db2\uff08PBS\uff09\u53ca\u4e59\u9187\u6d41\u52a8\u65b9\u5411\u3002<\/p>\n

(b) (a)\u56fe\u4e2d\u6807\u8bb0\u533a\u57df\u7684\u53d1\u5c04\u5149\u8c31\u3002\u80cc\u666f\u989c\u8272\u8868\u660e\u4e86(a)\u56fe\u4e2d\u6240\u7528\u7684\u53d1\u5c04\u6ee4\u8272\u955c\u79cd\u7c7b\u3002<\/p>\n

(c) \u5fae\u6d41\u63a7\u88c5\u7f6e\u8154\u5185\u672b\u5c3e\u6bb5\u62cd\u6444\u56fe\u3002\u56fe\u4e2d\u6240\u6709\u56fe\u7247\u5c3a\u5bf8\u5747\u4e3a1.6\u00d70.8 mm\u3002<\/p>\n

\u56fe3 \u5bf9PLGA-b-PEG\u7eb3\u7c73\u9897\u7c92\u7684\u5f62\u6210\u53ca\u81ea\u53d1\u8f7d\u836f\u8fc7\u7a0b\u7684\u5b9e\u65f6\u76d1\u6d4b<\/strong><\/p>\n

\"\"<\/a>
\n(a-c) \u758f\u6c34\u6027Cy5-OLA \u578b\u836f\u7269\u3002<\/p>\n

(d-f) \u4eb2\u6c34\u6027sulfo-Cy5 \u578b\u836f\u7269\u3002<\/p>\n

(a,d) \u8f7d\u836f\u53caFRET\u673a\u5236\u793a\u610f\u56fe\u3002<\/p>\n

(b,e) \u5fae\u6d41\u63a7\u88c5\u7f6e\u8154\u5185\u672b\u5c3e\u6bb5FRET\/Cy3.5 \u5f3a\u5ea6\u6bd4\u3002\u56fe\u7247\u5c3a\u5bf8\u4e3a1.6\u00d70.8 mm\u3002<\/p>\n

(c,f) (b,e)\u56fe\u4e2d\u6807\u8bb0\u533a\u57df\u7684\u53d1\u5c04\u5149\u8c31\u3002\u80cc\u666f\u989c\u8272\u8868\u660e\u4e86(b,e)\u56fe\u4e2d\u6240\u7528\u7684\u53d1\u5c04\u6ee4\u8272\u955c\u79cd\u7c7b\u3002<\/p>\n

\u56fe4 \u5bf9\u542b\u91cf\u5b50\u70b9\u6838\u91cd\u7ec4\u9ad8\u5bc6\u5ea6\u8102\u86cb\u767d\u5f62\u6210\u8fc7\u7a0b\u7684\u5b9e\u65f6\u76d1\u6d4b<\/strong><\/p>\n

\"\"<\/a>
\n(a) \u9ad8\u5bc6\u5ea6\u8102\u86cb\u767d\u81ea\u7ec4\u88c5\u53caFRET\u5f62\u6210\u8fc7\u7a0b\u793a\u610f\u56fe\u3002<\/p>\n

(b) \u9ad8\u5bc6\u5ea6\u8102\u86cb\u767d\u7ec4\u88c5\u671f\u95f4\u4e8e\u5fae\u6d41\u63a7\u88c5\u7f6e\u8154\u5185\u672b\u5c3e\u6bb5\u62cd\u6444\u7684\u5171\u805a\u7126\u56fe\uff0c\u6b64\u65f6\u6d41\u901f\u4e3a15 mL\/min\uff0c\u56fe\u7247\u5c3a\u5bf8\u4e3a1.6\u00d70.8 mm\u3002<\/p>\n

(c) \u5fae\u6d41\u63a7\u88c5\u7f6e\u53caFRET\u6210\u50cf\u88c5\u7f6e\u793a\u610f\u56fe\u3002<\/p>\n

(d) \u4e0d\u540c\u56de\u8def\u957f\u5ea6\u4e2d\u89c2\u5bdf\u82af\u7247\u7684FRET\/Cy3.5\u5f3a\u5ea6\u6bd4\u53ca\u5bf9\u5e94\u7684\u53d1\u5c04\u5149\u8c31\u3002<\/p>\n

\u3010\u5c0f\u7ed3\u3011<\/strong><\/p>\n

\u7efc\u4e0a\uff0c\u672c\u6587\u63d0\u51fa\u4e86\u4e00\u79cd\u901a\u8fc7\u7ed3\u5408\u5fae\u6d41\u63a7\u7eb3\u7c73\u7c92\u5b50\u5408\u6210\u6cd5\u53caFRET\u663e\u5fae\u6210\u50cf\u6280\u672f\u5b9e\u73b0\u7684\u7eb3\u7c73\u9897\u7c92\u81ea\u7ec4\u88c5\u8fc7\u7a0b\u7684\u5b9e\u65f6\u53ef\u89c6\u5316\u65b9\u6cd5\uff0c\u5e76\u5bf9\u4e09\u79cd\u4e0d\u540c\u7684\u7eb3\u7c73\u7cfb\u7edf\u8fdb\u884c\u6d4b\u8bd5\u3002\u8be5\u65b9\u6cd5\u6709\u52a9\u4e8e\u4eba\u4eec\u5bf9\u5404\u79cd\u7c7b\u578b\u7eb3\u7c73\u9897\u7c92\u7684\u5408\u6210\u3001\u5f62\u6210\u673a\u5236\u4ee5\u53ca\u529f\u80fd\u8fdb\u884c\u66f4\u6df1\u5c42\u6b21\u7684\u7814\u7a76\u3002<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1a<\/strong>Real-Time Monitoring of Nanoparticle Formation by FRET Imaging<\/a>\uff08Angew. Chem. Int. Ed.\uff0c2017\uff0c10.1002\/anie.201611288\uff09<\/p>\n

\u672c\u6587\u7531\u6750\u6599\u4eba\u7f16\u8f91\u90e8\u7eb3\u7c73\u5c0f\u7ec4seleneh\u6574\u7406\u7f16\u8bd1\uff0c\u70b9\u6211\u52a0\u5165\u6750\u6599\u4eba\u7f16\u8f91\u90e8<\/a>\u3002<\/p>\n

\u6750\u6599\u6d4b\u8bd5\uff0c\u6570\u636e\u5206\u6790\uff0c\u4e0a\u6d4b\u8bd5\u8c37<\/a>\uff01<\/p>\n","protected":false},"excerpt":{"rendered":"

\u3010\u5f15\u8a00\u3011 FRET\uff08F\u00f6rster Resonance Energy Transfer\/Fluorescence Resonance Energy Transf…<\/p>\n","protected":false},"author":2494,"featured_media":63149,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[215],"tags":[33],"class_list":["post-63133","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-jiedu","tag-nano"],"_links":{"self":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/63133"}],"collection":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/users\/2494"}],"replies":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=63133"}],"version-history":[{"count":12,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/63133\/revisions"}],"predecessor-version":[{"id":63414,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/63133\/revisions\/63414"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/media\/63149"}],"wp:attachment":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=63133"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=63133"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=63133"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}