{"id":38026,"date":"2016-11-23T06:30:43","date_gmt":"2016-11-22T22:30:43","guid":{"rendered":"\/\/m.iemloyee.com\/?p=38026"},"modified":"2016-11-22T16:24:52","modified_gmt":"2016-11-22T08:24:52","slug":"%e5%ad%a6%e6%9c%af%e5%b9%b2%e8%b4%a7%e4%b8%a8%e5%bd%b1%e5%93%8dgpcsec%e6%b5%8b%e8%af%95%e7%9a%84%e5%9b%a0%e7%b4%a0","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=38026","title":{"rendered":"\u5b66\u672f\u5e72\u8d27\u4e28\u5f71\u54cdGPC\/SEC\u6d4b\u8bd5\u7684\u56e0\u7d20"},"content":{"rendered":"

\u5f15\u8a00<\/strong>\uff1a<\/span><\/p>\n

GPC \u7528\u6237\u7ecf\u5e38\u4f1a\u9047\u5230\u8fd9\u6837\u7684\u95ee\u9898\uff1a\u901a\u8fc7GPC\u5f97\u5230\u7684\u5e73\u5747\u5206\u5b50\u91cf\u548c\u9884\u60f3\/\u4f30\u7b97\u7684\u5206\u5b50\u91cf\u5dee\u522b\u5f88\u5927\u3002\u5728GPC\u7cfb\u7edf\u6d4b\u8bd5\u4e2d\u5bfb\u627e\u89e3\u51b3\u8fd9\u4e2a\u95ee\u9898\u7684\u7b54\u6848\u662f\u4e00\u4e2a\u8270\u5de8\u7684\u4efb\u52a1\uff0c\u4f46\u662f\u5fc5\u9700\u53bb\u9762\u5bf9\u3002\u6211\u4eec\u7684\u4efb\u52a1\u662f\u53bb\u5bfb\u627e\u4e00\u4e2a\u7b26\u5408\u903b\u8f91\u7684\u65b9\u5f0f\uff0c\u901a\u8fc7\u68c0\u9a8c\u90a3\u4e9b\u5f71\u54cdGPC\u6d4b\u8bd5\u7684\u91cd\u8981\u56e0\u7d20\uff0c\u6700\u5927\u7a0b\u5ea6\u7684\u51cf\u5c0fGPC\u6d4b\u8bd5\u8fc7\u7a0b\u4e2d\u53ef\u80fd\u4f1a\u4ea7\u751f\u7684\u8bef\u5dee\uff0c\u4f7f\u5f97\u6d4b\u8bd5\u7ed3\u679c\u7684\u51c6\u786e\u6027\u5f97\u5230\u4fdd\u969c\u3002<\/p>\n

Gel Permeation Chromatography (GPC)\uff0c\u4e5f\u79f0\u4f5cSize Exclusion Chromatography (SEC) \u6216\u8005Gel Filtration Chromatography (GFC)\uff0c\u662f\u4e00\u79cd\u6839\u636e\u5c3a\u5bf8\u5206\u79bb\u9ad8\u5206\u5b50\u6750\u6599\u7684\u8272\u8c31\u6280\u672f\u3002GPC\u67f1\u5b50\u5206\u79bb\u539f\u7406\u662f\u57fa\u4e8e\u5206\u5b50\u5927\u5c0f\u800c\u4e0d\u662f\u5206\u5b50\u91cf\uff08Mw\uff09\uff0c\u5f53\u6837\u54c1\u4ece\u8272\u8c31\u67f1\u4e2d\u6d17\u8131\u51fa\u6765\uff0c\u4f7f\u7528\u4e00\u4e2a\u6216\u591a\u4e2a\u68c0\u6d4b\u5668\u5bf9\u5176\u8fdb\u884c\u68c0\u6d4b\uff0c\u901a\u8fc7\u4f20\u7edf\u6821\u6b63\u66f2\u7ebf\uff0c\u901a\u7528\u6821\u6b63\u66f2\u7ebf\u6216\u8005\u591a\u68c0\u6d4b\u5668\u8054\u7528\u8ba1\u7b97\u5f97\u5230\u4e0d\u540c\u7684\u7269\u7406\u53c2\u6570\u7684\u5206\u5e03\uff0c\u6bd4\u5982\u5206\u5b50\u91cf\uff0c\u5206\u5b50\u5c3a\u5bf8\uff08\u5305\u62ec\u56de\u65cb\u534a\u5f84\uff08Rg\uff09\u548c\u6d41\u4f53\u529b\u5b66\u4f53\u79ef\u534a\u5f84\uff08Rh\uff09\uff09\uff0c\u7c98\u5ea6\u7279\u6027\uff0c\u540c\u65f6\u8fd8\u6709\u9ad8\u5206\u5b50\u7ed3\u6784\uff0c\u6784\u8c61\uff0c\u7f14\u5408\uff0c\u5171\u805a\u7ec4\u6210\u7b49\u4fe1\u606f\u3002<\/p>\n

GPC\u6280\u672f<\/strong>\uff1a<\/span><\/p>\n

\u4f20\u7edf\u6821\u6b63\u66f2\u7ebf<\/strong>\uff1a\u4f7f\u7528\u4e00\u4e2a\u6d53\u5ea6\u68c0\u6d4b\u5668\u548c\u4e00\u4e2a\u6821\u6b63\u7684GPC\u67f1\u5b50\uff0c\u63d0\u4f9b\u76f8\u5bf9\u5206\u5b50\u91cfMw\u548c\u5206\u6563\u7a0b\u5ea6\u3002\u4f18\u70b9\uff1a\u7b80\u5355\u3001\u4fbf\u6377\u3001\u5e7f\u6cdb\u4f7f\u7528\u3001\u76f8\u5bf9\u4fbf\u5b9c\u3002\u7f3a\u70b9\uff1a\u68c0\u6d4b\u6837\u54c1\u53ea\u6709\u548c\u6807\u6837\u4e3a\u76f8\u540c\u7269\u8d28\u5206\u5b50\u91cf\u624d\u51c6\u786e\u3001\u5ffd\u7565\u7ed3\u6784\u5dee\u5f02\uff0c\u6240\u6709\u7684\u6807\u6837\u548c\u6837\u54c1\u90fd\u5047\u60f3\u4e3a\u7ebf\u6027\u7ed3\u6784\uff0c\u4e0d\u8003\u8651\u652f\u94fe\uff0c\u7f14\u5408\u548c\u5176\u4ed6\u7ed3\u6784\u4fee\u9970\u3002<\/p>\n

\u901a\u7528\u6821\u6b63\u66f2\u7ebf<\/strong>\uff1a\u4f7f\u7528\u7c98\u5ea6\u68c0\u6d4b\u5668\uff0c\u6d53\u5ea6\u68c0\u6d4b\u5668\u548c\u6821\u51c6\u7684GPC\u67f1\u5b50\uff0c\u63d0\u4f9b\u771f\u5b9e\u5206\u5b50\u91cf\uff0c\u5206\u5b50\u5c3a\u5bf8\uff0c\u7279\u6027\u7c98\u5ea6\uff0c\u4ee5\u53ca\u6784\u8c61\uff0c\u7f14\u5408\uff0c\u652f\u5316\u5ea6\u4fe1\u606f\u3002\u4f18\u70b9\uff1a\u63d0\u4f9b\u4e86\u771f\u5b9e\u7684\u4e0d\u4f9d\u8d56\u4e8e\u6807\u51c6\u7269\u7684\u5206\u5b50\u91cf\uff0c\u7c98\u5ea6\u68c0\u6d4b\u5668\u63d0\u4f9b\u4e86\u5176\u4ed6\u9644\u52a0\u4fe1\u606f\uff0c\u5982\u7ed3\u6784\u3001\u6784\u8c61\u3001\u7f14\u5408\u3001\u63a5\u679d\uff0c\u8fd9\u4e9b\u90fd\u662f\u4f20\u7edf\u6821\u6b63\u6240\u4e0d\u80fd\u63d0\u4f9b\u7684\u3002<\/p>\n

\u591a\uff08\u4e09\u6216\u56db\uff09\u68c0\u6d4b\u5668\u8054\u7528<\/strong>\uff1a\u4f7f\u7528\u5149\u6563\u5c04\u68c0\u6d4b\u5668\uff0c\u7c98\u5ea6\u68c0\u6d4b\u5668\uff0c\u6298\u5149\u6307\u6570\u68c0\u6d4b\u5668\uff0c\u6216\u7d2b\u5916\u68c0\u6d4b\u5668\u3002\u63d0\u4f9b\u7edd\u5bf9\u5206\u5b50\u91cf\uff0c\u5206\u5b50\u5c3a\u5bf8\uff0c\u7279\u6027\u7c98\u5ea6\uff0c\u4ee5\u53ca\u6784\u8c61\uff0c\u7f14\u5408\uff0c\u652f\u5316\u5ea6\u7b49\u4fe1\u606f\u3002\u4f18\u70b9\uff1a\u5149\u6563\u5c04\u68c0\u6d4b\u5668\u63d0\u4f9b\u4e86\u7edd\u5bf9\u5206\u5b50\u91cf\u4e0d\u9700\u8981\u8272\u8c31\u67f1\u6821\u6b63\uff0c\u7c98\u5ea6\u68c0\u6d4b\u5668\u540c\u65f6\u63d0\u4f9b\u7279\u6027\u7c98\u5ea6\u548c\u7ed3\u6784\u4fe1\u606f\uff0c\u5982\u6784\u578b\uff0c\u94fe\u521a\u6027\uff0c\u652f\u5316\u5ea6\uff0c\u7f14\u5408\u7b49\uff0c\u5bf9\u4e8eRg>10 nm\u7684\u9ad8\u5206\u5b50\u53ef\u68c0\u6d4bRg\uff0c\u53ef\u68c0\u6d4b\u4efb\u610f\u5c3a\u5bf8\u7684\u9ad8\u5206\u5b50\u6750\u6599\u7684Rh\u3002<\/p>\n

\u591a\u68c0\u6d4b\u5668\u8054\u7528GPC\u786c\u4ef6\u914d\u7f6e\u793a\u610f\u56fe<\/strong>\uff1a<\/span><\/p>\n

 <\/p>\n

\"%e5%a4%9a%e6%a3%80%e6%b5%8b%e5%99%a8%e8%81%94%e7%94%a8gpc%e7%a1%ac%e4%bb%b6%e9%85%8d%e7%bd%ae%e7%a4%ba%e6%84%8f%e5%9b%be-2\"<\/a><\/p>\n

GPC \u9a8c\u8bc1\u8fc7\u7a0b\u4e2d\u7684\u8d28\u91cf\u63a7\u5236<\/strong><\/span><\/p>\n

\uff081\uff09 \u627e\u5230 \u201c\u57fa\u672c\u95ee\u9898\u5143\u7d20\u201d<\/strong> \uff1a\u8ba1\u7b97\u6240\u9700\u53c2\u6570\uff0c\u8ba1\u7b97\u8fc7\u7a0b \uff1b\u6d41\u901f\uff0c\u6837\u54c1\u6d53\u5ea6\uff0c\u8fdb\u6837\u4f53\u79ef\uff0c\u8272\u8c31\u67f1 \uff1b\u4eea\u5668\u51b2\u6d17\uff0c\u6e05\u6d01\uff0c\u566a\u58f0\u6c34\u5e73\u3002<\/p>\n

\uff082\uff09 \u901a\u8fc7\u6807\u51c6\u6837\u54c1\u68c0\u67e5\u4eea\u5668\u7684\u6027\u80fd<\/strong> \uff1a\u4eea\u5668\u5e38\u6570\uff0c\u4fe1\u53f7\u54cd\u5e94\u3002<\/p>\n

\uff083\uff09\u6570\u636e\u5904\u7406 \u2013 \u9a8c\u8bc1<\/strong> \uff1a\u6d4b\u8bd5\u4e00\u4e2a\u901a\u5e38\u4f7f\u7528\u7684\u6807\u51c6\u6837\u54c1\u7528\u4e8e\u68c0\u9a8c\u3002<\/p>\n

\u76f4\u63a5\u53d8\u91cf<\/strong>\uff1a\uff08\u57fa\u672c\u8003\u8651\u56e0\u7d20\uff09\u8d28\u91cf, \u7c98\u5ea6\u8ba1\u548c\u5149\u6563\u5c04\u68c0\u6d4b\u5668\u5e38\u6570, \u6821\u6b63\u66f2\u7ebf\u3002<\/p>\n

\u4e2d\u95f4\u53d8\u91cf<\/strong>\uff1a\uff08\u548c\u76f4\u63a5\u53d8\u91cf\u540c\u6837\u91cd\u8981\uff09 dn\/dc, \u6d41\u901f, \u6837\u54c1\u6d53\u5ea6, \u8fdb\u6837\u4f53\u79ef\uff1b\u5728\u4e00\u4e2a\u7edd\u5bf9\u7684\u6d4b\u8bd5\u8fc7\u7a0b\u4e2d\uff0c\u6240\u6709\u7684\u53d8\u91cf\u90fd\u5fc5\u987b\u662f\u7edd\u5bf9\u6570\u503c\u3002<\/p>\n

\u6027\u80fd\u53c2\u6570<\/strong>\uff1a\uff08\u51c6\u786e\u6027\u548c\u7cbe\u786e\u6027\uff09\u6cf5\u566a\u58f0\uff0c\u68c0\u6d4b\u5668\u566a\u58f0\uff0c\u8f6f\u4ef6\u6574\u5408\uff0c\u8272\u8c31\u67f1\uff0c\u8fdb\u6837\u6280\u672f\u3002<\/p>\n

\u6d41\u901f\u7684\u8d21\u732e<\/strong><\/span><\/p>\n

\uff081\uff09 \u5f71\u54cd\u5206\u8fa8\u7387<\/strong>\uff1a\u592a\u9ad8\u6216\u8005\u592a\u4f4e\u7684\u6d41\u901f\u5bf9\u4e8e\u8f74\u5411\u6e17\u900f\u548c\u5b54\u5f84\u4fdd\u7559\u4e0d\u5229 (\u4fdd\u6301\u6d41\u901f\u5728 0.5 – 1.5 mL\/min: \u6ce8\u610f\u6eb6\u5242\u7684\u7c98\u5ea6)\uff0c\u8d85\u8fc7 3% \u7684\u6539\u53d8\uff0c\u5c06\u5f71\u54cd\u8272\u8c31\u67f1\u7684\u6027\u80fd\u3002<\/p>\n

\uff082\uff09 \u5f71\u54cd\u901a\u8fc7\u6821\u6b63\u66f2\u7ebf\u7684\u5230\u7684\u7ed3\u679c<\/strong>\uff1a1% \u7684\u6d41\u901f\u8bef\u5dee\u9020\u6210 10% \u4ee5\u4e0a\u7684\u5206\u5b50\u91cf\u8bef\u5dee\u3002<\/p>\n

\uff083\uff09\u5f71\u54cd\u6d53\u5ea6\uff0c\u7c98\u5ea6\u548c\u5149\u6563\u5c04\u68c0\u6d4b\u5668\u8ba1\u7b97<\/strong>\uff1a\u4ea7\u751f\u7684\u8bef\u5dee\u6b63\u6bd4\u4e8e\u6d41\u901f\u8bef\u5dee\u3002<\/p>\n

\u6d53\u5ea6\u7684\u8d21\u732e<\/strong><\/span><\/p>\n

\uff081\uff09 \u5f71\u54cd\u5206\u8fa8\u7387<\/strong>\uff1a\u7c98\u6027\u6307\u8fdb\/Viscous Fingering\uff0c\u8272\u8c31\u67f1\u8fc7\u91cf\u4e0a\u6837\u3002<\/p>\n

\uff082\uff09 \u5bf9\u901a\u7528\u6821\u6b63\u7684\u5f71\u54cd<\/strong>\uff1a\u7c98\u5ea6\u8bef\u5dee\u6b63\u6bd4\u4e8e\u6d53\u5ea6\u8bef\u5dee\uff0c\u5206\u5b50\u91cf\u8bef\u5dee\u548c\u6d53\u5ea6\u8bef\u5dee\u5012\u6570\u6210\u6b63\u6bd4 \uff0cMark-Houwink \u8bef\u5dee\u548c\u6d53\u5ea6\u8bef\u5dee\u7684\u5e73\u65b9\u6210\u6b63\u6bd4\u3002<\/p>\n

\uff083\uff09 \u5bf9\u4e8e\u5149\u6563\u5c04\u7684\u5f71\u54cd<\/strong>\uff1a \u5206\u5b50\u91cf\u8bef\u5dee\u6b63\u6bd4\u4e8e\u6d53\u5ea6\u8bef\u5dee\uff0cdn\/dc \u8bef\u5dee\u548c\u5206\u5b50\u91cf\u8bef\u5dee\u7684\u5e73\u65b9\u6210\u6b63\u6bd4\u3002<\/p>\n

\u8fdb\u6837\u4f53\u79ef\u7684\u8d21\u732e<\/strong><\/span><\/p>\n

\uff081\uff09 \u5f71\u54cd\u5206\u8fa8\u7387<\/strong>\uff1a\u7c98\u6027\u6307\u8fdb\/Viscous Fingering\uff0c\u8272\u8c31\u67f1\u8fc7\u91cf\u4e0a\u6837\uff0c\u521d\u59cb\u5206\u6563\uff0c\u8fdb\u6837\u4f53\u79ef\u8d8a\u5927\uff1a\u7cbe\u786e\u6027\uff08\u91cd\u590d\u6027\uff09\u589e\u52a0\uff0c\u51c6\u786e\u6027\u4e0b\u964d\u3002<\/p>\n

\uff082\uff09\u8ba1\u7b97\u8fc7\u7a0b\u4e2d\u7684\u8bef\u5dee<\/strong>\uff1a\u548c\u6d53\u5ea6\u8bef\u5dee\u76f8\u540c\u3002<\/p>\n

dn\/dc \u7684\u8d21\u732e<\/strong><\/span><\/p>\n

\uff081\uff09 dn\/dc \u5f71\u54cd RI \u4fe1\u53f7<\/strong>\uff1a\u5f88\u5c0f\u7684 dn\/dc \u610f\u5473\u7740 RI \u4fe1\u53f7\u5f88\u4f4e\uff0c\u5bf9\u4e8e\u5bbd\u5206\u5e03\u6837\u54c1\u7684\u6d4b\u8bd5\u5f71\u54cd\u6700\u5927\uff0c\u5206\u5e03\u5bbd\uff0c\u96be\u79ef\u5206\u3002<\/p>\n

\uff082\uff09 dn\/dc\u5927\u5c0f \u4e25\u91cd\u5f71\u54cd LS \u4fe1\u53f7<\/strong>\uff1a\u5149\u6563\u5c04\u4fe1\u53f7\u6b63\u6bd4\u4e8e (dn\/dc)2\u3002<\/p>\n

\uff083\uff09dn\/dc \u4f1a\u626d\u66f2 RI \u548c LS \u4fe1\u53f7<\/strong>\uff1a\u65e0\u89c4\u5171\u805a\u7269\u7684 dn\/dc \u968f\u5206\u5b50\u91cf\u6539\u53d8\u800c\u6539\uff0cRI + LS \u4e0d\u80fd\u76f4\u63a5\u68c0\u6d4b\u5230\u5206\u5b50\u91cf\u548c\u6d53\u5ea6\uff0c\u4ea4\u66ff\u5171\u805a\u7269\u6ca1\u6709\u8fd9\u4e2a\u95ee\u9898\u3002<\/p>\n

\u5b9e\u9645\u5f71\u54cd\u56e0\u7d20<\/strong><\/span><\/p>\n

\uff081\uff09 \u7c98\u5ea6\u68c0\u6d4b\u5668\u5bf9\u4e8e\u9897\u7c92\u7269\u7684\u5b58\u5728\u5e76\u4e0d\u654f\u611f\uff0c\u4f46\u662f\u5bf9\u4e8e\u6c14\u6ce1\u5341\u5206\u654f\u611f<\/strong>\uff1a\u6c14\u6ce1\u5bfc\u81f4\u6bdb\u7ec6\u7ba1\u4e2d\u7684\u4ecb\u8d28\u7684\u53ef\u538b\u7f29\u6027\u589e\u52a0\uff1a\u8fd9\u79cd\u53ef\u538b\u7f29\u6027\u53ef\u4ee5\u8f6c\u5316\u4e3a\u5f88\u5f3a\u7684\u566a\u58f0\uff0c\u9700\u8981\u9002\u5f53\u7684\u6eb6\u5242\u8131\u6c14\u3002\u6c14\u6ce1\u540c\u65f6\u5bfc\u81f4\u8272\u8c31\u67f1\u964d\u7ea7\uff1a\u8272\u8c31\u67f1\u5185\u51dd\u80f6\u586b\u6599\u7684\u81a8\u80c0\u548c\u73af\u5883\u6eb6\u5242\u76f8\u5173\u3002<\/p>\n

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