{"id":37136,"date":"2016-09-01T08:00:45","date_gmt":"2016-09-01T00:00:45","guid":{"rendered":"\/\/m.iemloyee.com\/?p=37136"},"modified":"2016-08-31T23:23:53","modified_gmt":"2016-08-31T15:23:53","slug":"adv-mater-%e6%b5%8e%e5%8d%97%e5%a4%a7%e5%ad%a6-%e5%88%a9%e7%94%a8%e6%af%94%e7%8e%87%e8%8d%a7%e5%85%89%e6%8e%a2%e9%92%88%e5%ae%9e%e7%8e%b0%e5%9c%a8%e4%bd%93%e5%86%85%e5%af%b9h2o2%e7%9a%84%e8%bf%91","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=37136","title":{"rendered":"Adv. Mater. \u6d4e\u5357\u5927\u5b66-\u5229\u7528\u6bd4\u7387\u8367\u5149\u63a2\u9488\u5b9e\u73b0\u5728\u4f53\u5185\u5bf9H2O2\u7684\u8fd1\u7ea2\u5916\u548c\u53cc\u5149\u5b50\u6210\u50cf"},"content":{"rendered":"

\u3010\u5f15\u8a00\u3011<\/strong><\/p>\n

\u6d3b\u6027\u6c27\u7c07\uff08ROS\uff09\u662f\u4e00\u7c7b\u5bf9\u751f\u7269\u5206\u5b50\u5177\u6709\u5f88\u9ad8\u53cd\u5e94\u6027\u7684\u542b\u6c27\u5206\u5b50\uff0cROS\u5728\u5f88\u591a\u751f\u7406\u548c\u75c5\u7406\u5b66\u8fdb\u7a0b\u4e2d\u626e\u6f14\u7740\u91cd\u8981\u89d2\u8272\u3002\u8fc7\u6c27\u5316\u6c22\uff08H2O2\uff09\u662f\u4e3b\u8981\u7684\u4e00\u79cdROS\uff0c\u5b83\u662f\u7ec6\u80de\u751f\u957f\u3001\u7e41\u6b96\u548c\u5206\u5316\u7684\u91cd\u8981\u4fe1\u4f7f\u3002\u4f46\u662f\u8fc7\u91cf\u7684H2O2\u901a\u5e38\u9884\u793a\u7740\u75be\u75c5\uff0c\u5982\u764c\u75c7\u3001\u795e\u7ecf\u8870\u9000\u548c\u5fc3\u8840\u7ba1\u75be\u75c5\u7b49\u3002\u56e0\u6b64\u53d1\u5c55\u80fd\u68c0\u6d4b\u4f53\u5185\u7684H2O2\u7684\u65b9\u6cd5\u81f3\u5173\u91cd\u8981\u3002<\/p>\n

\u8367\u5149\u63a2\u9488\u662f\u751f\u7269\u5206\u5b50\u68c0\u6d4b\u5e38\u7528\u7684\u624b\u6bb5\uff0c\u4f46\u662f\u76ee\u524d\u7684\u8367\u5149\u63a2\u9488\u5927\u591a\u662f\u57fa\u4e8e\u5f3a\u5ea6\u578b\u7684\uff08\u8367\u5149\u5f3a\u5ea6\u53d8\u5f3a\u6216\u53d8\u5f31\uff09\u3002\u4e3a\u4e86\u89e3\u51b3\u8fd9\u4e00\u95ee\u9898\uff0c\u5df2\u7ecf\u7814\u7a76\u51fa\u4e86\u51e0\u79cd\u9488\u5bf9H2O2\u7684\u6bd4\u7387\u8367\u5149\u63a2\u9488\uff0c\u4f46\u662f\u8fd8\u5b58\u5728\u51e0\u4e2a\u7f3a\u70b9\uff1a\u7b2c\u4e00\uff0c\u7279\u5f02\u6027\u4e0d\u5f3a\uff0c\u9664\u4e86\u8ddfH2O2\u53cd\u5e94\u5916\u8fd8\u80fd\u8ddfNO\u53cd\u5e94\uff1b\u7b2c\u4e8c\uff0c\u8fd9\u4e9b\u63a2\u9488\u7ecf\u5e38\u662f\u5355\u5149\u5b50\u6fc0\u53d1\u6216\u8005\u53d1\u5c04\u7684\u8367\u5149\u5728\u53ef\u89c1\u5149\u8303\u56f4\u5185\uff0c\u7ec4\u7ec7\u7a7f\u900f\u80fd\u529b\u6709\u9650\uff1b\u7b2c\u4e09\uff0c\u53d1\u5c04\u4f4d\u79fb\u5c0f\uff0c\u4f7f\u5f97\u63a2\u9488\u548c\u53cd\u5e94\u4ea7\u7269\u7684\u8367\u5149\u76f8\u4e92\u91cd\u53e0\u3002<\/p>\n

\u3010\u6210\u679c\u7b80\u4ecb\u3011<\/strong><\/p>\n

\u6d4e\u5357\u5927\u5b66\u8367\u5149\u63a2\u9488\u4e0e\u751f\u7269\u533b\u5b66\u6210\u50cf\u7814\u7a76\u6240\u7684\u6797\u4f1f\u82f1\u6559\u6388\uff08\u901a\u8baf\u4f5c\u8005\uff09\u7684\u56e2\u961f\u8bbe\u8ba1\u4e86\u4e00\u79cd\u6bd4\u7387\u8367\u5149\u63a2\u9488\u6765\u5bf9H2O2\u8fdb\u884c\u540c\u65f6\u7684\u8fd1\u7ea2\u5916\u548c\u53cc\u5149\u5b50\u6210\u50cf\uff08\u4e0b\u6587\u7528BC\u4ee3\u8868\u6587\u4e2d\u8bbe\u8ba1\u7684\u63a2\u9488\uff09\u3002BC\u542b\u6709\u7279\u6b8a\u7684H2O2\u53cd\u5e94\u4f4d\u70b9\uff0c\u80fd\u4e0eH2O2\u53cd\u5e94\uff08\u5176\u5b83\u751f\u7269\u5206\u5b50\u5219\u51e0\u4e4e\u4e0d\u53cd\u5e94\uff09\uff0c\u53cd\u5e94\u540eBC 472nm\u5904\u7684\u8367\u5149\u5f3a\u5ea6\u589e\u52a0\uff0c\u800c693nm\u5904\u7684\u8367\u5149\u5f3a\u5ea6\u5219\u51cf\u5f31\uff0c\u8fd9\u4e24\u79cd\u8367\u5149\u7684\u6bd4\u4f8b\u4e0eH2O2\u7684\u6d53\u5ea6\u5448\u7ebf\u6027\u5173\u7cfb\u3002\u540c\u65f6BC\u4e0eH2O2\u53cd\u5e94\u540e\u53ef\u4ee5\u91ca\u653e\u4e00\u79cd\u53cc\u5149\u5b50\u8367\u5149\u67d3\u6599\uff0c\u67d3\u6599\u53ef\u4ee5\u5438\u6536760nm\u7684\u8fd1\u7ea2\u5916\u5149\u800c\u53d1\u51fa\u7eff\u5149\uff0c\u4ece\u800c\u5bf9H2O2\u8fdb\u884c\u6210\u50cf\u3002BC\u5bf9H2O2\u5177\u6709\u5f88\u9ad8\u7684\u9009\u62e9\u6027\uff0c\u53ef\u4ee5\u62d3\u5c55\u7528\u6765\u5bf9\u5176\u5b83ROS\u8fdb\u884c\u6210\u50cf\u3002<\/p>\n

\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/strong><\/p>\n

\u56fe1 \u8367\u5149BC\u4e0eH2O2\u53cd\u5e94\u5bfc\u81f4\u7684\u5438\u6536\u548c\u53d1\u5c04\u5149\u7684\u53d8\u5316<\/strong><\/p>\n

\"\u56fe\u72472\"<\/p>\n

\uff08A\uff09\u6d53\u5ea6\u4e3a 5 \u00d7 10\u22126 m \u7684BC\u5728\u4e0d\u540cH2O2\u6d53\u5ea6\uff080-180\u5f53\u91cf\uff09\u65f6\u7684\u5438\u6536\u5149\u8c31\uff0c\u63d2\u56fe\uff1a\u81ea\u7136\u5149\u4e0bH2O2\u542b\u91cf\u5206\u522b\u4e3a0\u548c120\u5f53\u91cf\u7684\u6eb6\u6db2\u7684\u7167\u7247\u3002<\/p>\n

\uff08B\uff09PBS\u6eb6\u6db2\u4e2d\uff0c\u6d53\u5ea6\u4e3a 5 \u00d7 10\u22126 m \u7684BC\u5728\u4e0d\u540cH2O2\u6d53\u5ea6\u65f6\u7684\u8367\u5149\u5149\u8c31\uff08\u6fc0\u53d1\u5149\u6ce2\u957f\u4e3a410nm\uff09\uff0c\u63d2\u56fe\uff1a 472 \u548c693 nm\u5904\u8367\u5149\u5f3a\u5ea6\u4e0eH2O2\u6d53\u5ea6\u7684\u5173\u7cfb\uff1b<\/p>\n

\uff08C\uff09472nm\u548c693nm\u5149\u5f3a\u6bd4\u7387\u4e0eH2O2\u6d53\u5ea6\u7684\u7ebf\u6027\u5173\u7cfb\uff0c\u63d2\u56fe\uff1a365 nm\u7d2b\u5916\u5149\u7167\u5c04\u4e0bH2O2\u542b\u91cf\u5206\u522b\u4e3a0\u548c120\u5f53\u91cf\u7684\u6eb6\u6db2\u7684\u7167\u7247\uff1b<\/p>\n

\uff08D\uff09\u7528\u4e0d\u540c\u79cd\u7c7b\u5206\u5b50\u5904\u7406BC\u65f6472nm\u548c693nm\u7684\u5149\u5f3a\u6bd4\u7387\uff0c\u6fc0\u53d1\u5149\u6ce2\u957f\u4e3a410nm\u3002<\/p>\n

\u56fe2 \u7528BC\u68c0\u6d4bRAW 264.7\u5de8\u541e\u566c\u7ec6\u80de\u4ea7\u751f\u7684H2O2<\/strong><\/p>\n

\"\u56fe\u72473\"<\/p>\n

\uff08A\uff09\u5de8\u541e\u566c\u7ec6\u80de\u7684\u56fe\u7247\uff08a1-a4\uff09\u548c\u75285 \u00d7 10\u22126 m BC\u5904\u7406\u7684\u7ec6\u80de\u7684\u56fe\u7247\uff08b1-b4\uff09\u4ee5\u53ca\u75285 \u00d7 10\u22126 m BC + 3\u03bcg mL\u22121 PMA\u5904\u7406\u7684\u7ec6\u80de\u7684\u56fe\u7247\uff08c1-c4\uff0cPMA\u80fd\u523a\u6fc0\u4ea7\u751fH2O2\uff09\uff0c\u7eff\u8272\uff08a1-c1\uff09\u548c\u7ea2\u8272\uff08a2-c2\uff09\u8367\u5149\u901a\u9053\u4e3a\u5355\u5149\u5b50\u6210\u50cf\uff08OP\uff09\u6a21\u5f0f\uff0ca3-c3\u662f\u660e\u573a\u7ec6\u80de\u56fe\u7247\u4e0e\u7eff\u8272\u548c\u7ea2\u8272\u901a\u9053\u5408\u5e76\u7684\u56fe\u7247\uff0ca4-c3\u662f\u53cc\u5149\u5b50\u6210\u50cf\uff08TP\uff09\u6a21\u5f0f\u3002OP\u6210\u50cf\uff1a\u7eff\u8272\uff0c\u03bbex = 488 nm, \u03bbem = 500\u2013550 nm\uff1b\u7ea2\u8272\uff0c\u03bbex = 647 nm, \u03bbem = 663\u2013738 nm\uff1bTP\u6a21\u5f0f\uff0c \u03bbex = 760 nm, \u03bbem = 500\u2013550 nm\uff08\u03bbex\u4e3a\u6fc0\u53d1\u5149\u6ce2\u957f\uff0c\u03bbem\u4e3a\u53d1\u5c04\u8367\u5149\u6ce2\u957f\uff09\u3002<\/p>\n

\uff08B\uff09\u56feA\u4e2d\u76f8\u5bf9\u8367\u5149\u5f3a\u5ea6\u7684\u91cf\u5316\uff1b \u00a0 \u00a0\uff08C\uff09\u4ece\u56feB\u4e2d\u5f97\u5230\u7684\u7eff\u5149\u548c\u7ea2\u5149\u5f3a\u5ea6\u7684\u6bd4\u7387\u3002<\/p>\n

\u56fe3 \u7528\u6591\u9a6c\u9c7c\u5e7c\u866b\u68c0\u9a8cBC\u7684\u6210\u50cf\u80fd\u529b<\/strong><\/p>\n

\"\u56fe\u72474\"<\/p>\n

\uff08A\uff09\u6591\u9a6c\u9c7c\uff08a1-a4\uff09\u548c\u7528 10 \u00d7 10\u22126 m BC\uff08b1-b4\uff09\u4e0e10 \u00d7 10\u22126 m BC + 200 \u00d7 10\u22126 m H2O2\u5904\u7406\u6591\u9a6c\u9c7c\u7684\u56fe\u7247\uff1b\u7eff\u8272\uff08a1-c1\uff09\u548c\u7ea2\u8272\uff08a2-c2\uff09\u8367\u5149\u901a\u9053\u4e3a\u5355\u5149\u5b50\u6210\u50cf\uff08OP\uff09\u6a21\u5f0f\uff0ca3-c3\u662f\u660e\u573a\u7ec6\u80de\u56fe\u7247\u4e0e\u7eff\u8272\u548c\u7ea2\u8272\u901a\u9053\u5408\u5e76\u7684\u56fe\u7247\uff0ca4-c3\u662f\u53cc\u5149\u5b50\u6210\u50cf\uff08TP\uff09\u6a21\u5f0f\u3002OP\u6210\u50cf\uff1a\u7eff\u8272\uff0c\u03bbex = 488 nm, \u03bbem = 500\u2013550 nm\uff1b\u7ea2\u8272\uff0c\u03bbex = 647 nm, \u03bbem = 663\u2013738 nm\uff1bTP\u6a21\u5f0f\uff0c \u03bbex = 760 nm, \u03bbem = 500\u2013550 nm\uff08\u03bbex\u4e3a\u6fc0\u53d1\u5149\u6ce2\u957f\uff0c\u03bbem\u4e3a\u53d1\u5c04\u8367\u5149\u6ce2\u957f\uff09\u3002<\/p>\n

\uff08B\uff09\u56feA\u4e2d\u76f8\u5bf9\u8367\u5149\u5f3a\u5ea6\u7684\u91cf\u5316\uff1b \u00a0\uff08C\uff09\u4ece\u56feB\u4e2d\u5f97\u5230\u7684\u7eff\u5149\u548c\u7ea2\u5149\u5f3a\u5ea6\u7684\u6bd4\u7387\u3002<\/p>\n

\u6ce8\u89e3\u56fe\u793a1\uff1a \u6bd4\u7387\u8367\u5149BC\u7684\u8bbe\u8ba1\u8def\u7ebf\u56fe<\/strong><\/p>\n

\"\u56fe\u72471\"<\/p>\n

\u6ce8\u89e3\u56fe\u793a2\uff1a \u4e0d\u540c\u8367\u5149\u63a2\u9488\u7684\u5bf9\u6bd4<\/strong><\/p>\n

\"\u56fe\u72475\"<\/p>\n

\uff08A\uff09\u76ee\u524d\u6700\u901a\u7528\u7684\u4e24\u79cdH2O2\u63a2\u9488\uff1b\uff08B\uff09\u672c\u6587\u8bbe\u8ba1\u7684\u63a2\u9488<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1aSimultaneous Near-Infrared and Two-Photon In Vivo Imaging of H2O2\u00a0Using a Ratiometric Fluorescent Probe based on the Unique Oxidative Rearrangement of Oxonium<\/a>\uff08Adv. Mater., 2016, \u00a0DOI: 10.1002\/adma.201602939\uff09<\/p>\n

\u672c\u6587\u7531\u6750\u6599\u4eba\u751f\u7269\u6750\u6599\u5c0f\u7ec4CZM\u4f9b\u7a3f\uff0c\u6750\u6599\u725b\u7f16\u8f91\u6574\u7406\u3002<\/p>\n

\u53c2\u4e0e\u751f\u7269\u6750\u6599\u8bdd\u9898\u8ba8\u8bba\u6216\u4e86\u89e3\u751f\u7269\u6750\u6599\u5c0f\u7ec4\u62db\u52df\u8be6\u60c5\uff0c\u8bf7\u52a0\u5165\u6750\u6599\u4eba\u751f\u7269\u6750\u6599\u4ea4\u6d41\u7fa4\uff08124806506\uff09\u3002<\/p>\n

\u6750\u6599\u725b\u7f51\u4e13\u6ce8\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u53ca\u884c\u4e1a\u8fdb\u5c55\uff0c\u8fd9\u91cc\u6c47\u96c6\u4e86\u5404\u5927\u9ad8\u6821\u7855\u535a\u751f\u3001\u4e00\u7ebf\u79d1\u7814\u4eba\u5458\u4ee5\u53ca\u884c\u4e1a\u4ece\u4e1a\u8005\uff0c\u5982\u679c\u60a8\u5bf9\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u8fdb\u5c55\uff0c\u89e3\u8bfb\u9ad8\u6c34\u5e73\u6587\u7ae0\u6216\u662f\u8bc4\u8ff0\u884c\u4e1a\u6709\u5174\u8da3\uff0c\u70b9\u6211\u52a0\u5165\u7f16\u8f91\u90e8<\/a>\u3002<\/u><\/p>\n

\u6750\u6599\u4eba\u7f51\u5c0a\u91cd\u6240\u6709\u8fdb\u884c\u77e5\u8bc6\u4f20\u64ad\u7684\u5a92\u4f53\uff0c\u8f6c\u8f7d\u8bf7\u8054\u7cfbtougao@cailiaoren.com<\/p>\n","protected":false},"excerpt":{"rendered":"

\u3010\u5f15\u8a00\u3011 \u6d3b\u6027\u6c27\u7c07\uff08ROS\uff09\u662f\u4e00\u7c7b\u5bf9\u751f\u7269\u5206\u5b50\u5177\u6709\u5f88\u9ad8\u53cd\u5e94\u6027\u7684\u542b\u6c27\u5206\u5b50\uff0cROS\u5728\u5f88\u591a\u751f\u7406\u548c\u75c5\u7406\u5b66\u8fdb\u7a0b\u4e2d\u626e\u6f14\u7740\u91cd\u8981\u89d2\u8272\u3002\u8fc7\u6c27\u5316\u6c22\uff08H2O2\uff09\u662f\u4e3b\u8981\u7684\u4e00\u79cdROS\uff0c\u5b83\u662f…<\/p>\n","protected":false},"author":382,"featured_media":37141,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[215],"tags":[75],"class_list":["post-37136","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-jiedu","tag-biomaterial"],"_links":{"self":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/37136"}],"collection":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/users\/382"}],"replies":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=37136"}],"version-history":[{"count":8,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/37136\/revisions"}],"predecessor-version":[{"id":37760,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/37136\/revisions\/37760"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/media\/37141"}],"wp:attachment":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=37136"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=37136"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=37136"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}