{"id":264377,"date":"2025-01-06T06:18:53","date_gmt":"2025-01-05T22:18:53","guid":{"rendered":"\/\/m.iemloyee.com\/?p=264377"},"modified":"2025-01-05T21:19:09","modified_gmt":"2025-01-05T13:19:09","slug":"science%ef%bc%9a%e6%99%af%e8%a7%82%e5%88%86%e6%9e%90%e9%ab%98%e6%95%88%e7%ad%9b%e9%80%89pet%e6%b0%b4%e8%a7%a3%e9%85%b6","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=264377","title":{"rendered":"Science\uff1a\u666f\u89c2\u5206\u6790\u9ad8\u6548\u7b5b\u9009PET\u6c34\u89e3\u9176"},"content":{"rendered":"

\u4e00\u3001\u3010\u79d1\u5b66\u80cc\u666f\u3011<\/strong>\u00a0 <\/strong><\/p>\n

\u805a\u5bf9\u82ef\u4e8c\u7532\u9178\u4e59\u4e8c\u9187\u916f\uff08PET\uff09\u662f\u4e00\u79cd\u5e7f\u6cdb\u4f7f\u7528\u7684\u5851\u6599\u6750\u6599\uff0c\u56e0\u5176\u8010\u4e45\u6027\u548c\u5e7f\u6cdb\u7684\u5e94\u7528\u800c\u96be\u4ee5\u81ea\u7136\u964d\u89e3\uff0c\u5bfc\u81f4\u4e25\u91cd\u7684\u73af\u5883\u6c61\u67d3\u95ee\u9898\u3002\u8fd1\u5e74\u6765\uff0c\u79d1\u5b66\u5bb6\u4eec\u81f4\u529b\u4e8e\u5f00\u53d1\u80fd\u591f\u6709\u6548\u964d\u89e3PET\u7684\u9176\uff0c\u5373PET\u6c34\u89e3\u9176\uff08PETases\uff09\uff0c\u4ee5\u5b9e\u73b0\u5851\u6599\u7684\u751f\u7269\u56de\u6536\u5229\u7528\u3002<\/p>\n

\u5c3d\u7ba1\u5df2\u6709\u4e00\u4e9bPET\u964d\u89e3\u9176\u88ab\u53d1\u73b0\uff0c\u4f8b\u5982\u6765\u81ea\u7ec6\u83ccIdeonella sakaiensis\u7684IsPETase\u9176\u548c\u53f6\u5806\u80a5\u89d2\u8d28\u9176\uff08LCC\uff09\uff0c\u4f46\u8fd9\u4e9b\u9176\u7684\u964d\u89e3\u6548\u7387\u548c\u5de5\u4e1a\u5e94\u7528\u6f5c\u529b\u4ecd\u6709\u9650\u3002\u6b64\u5916\uff0c\u81ea\u7136\u754c\u4e2d\u53ef\u80fd\u5b58\u5728\u66f4\u591a\u5177\u6709PET\u964d\u89e3\u80fd\u529b\u7684\u9176\uff0c\u4f46\u76ee\u524d\u7f3a\u4e4f\u6709\u6548\u7684\u65b9\u6cd5\u6765\u8bc6\u522b\u548c\u8bc4\u4f30\u8fd9\u4e9b\u9176\u7684\u6f5c\u529b\u3002\u56e0\u6b64\uff0c\u672c\u6587\u65e8\u5728\u901a\u8fc7\u4e00\u79cd\u65b0\u7684\u65b9\u6cd5\u2014\u2014\u666f\u89c2\u5206\u6790\u6846\u67b6\uff0c\u6765\u7cfb\u7edf\u5730\u8bc4\u4f30\u548c\u7b5b\u9009\u5177\u6709PET\u964d\u89e3\u80fd\u529b\u7684\u9176\uff0c\u4ee5\u671f\u53d1\u73b0\u66f4\u9ad8\u6548\u3001\u66f4\u5177\u5de5\u4e1a\u5e94\u7528\u6f5c\u529b\u7684PET\u964d\u89e3\u9176\u3002<\/p>\n

\u4e8c\u3001\u3010\u521b\u65b0\u6210\u679c\u3011<\/strong><\/p>\n

\u8fd1\u65e5\uff0c\u97e9\u56fd\u7814\u7a76\u4eba\u5458\u5f00\u53d1\u4e86\u4e00\u79cd\u57fa\u4e8e\u81ea\u7136\u5e8f\u5217\u805a\u7c7b\u6846\u67b6\u7684\u666f\u89c2\u5206\u6790\u65b9\u6cd5\uff0c\u7528\u4e8e\u8bc4\u4f30\u548c\u7b5b\u9009\u5177\u6709PET\u964d\u89e3\u80fd\u529b\u7684\u9176\u3002\u8be5\u65b9\u6cd5\u901a\u8fc7\u6784\u5efa\u4e00\u4e2a\u5305\u542b170\u4e2a\u805a\u7c7b\u548c1894\u4e2a\u8282\u70b9\u7684\u8bed\u4e49\u7f51\u7edc\uff0c\u6db5\u76d6\u4e86\u5df2\u77e5\u7684\u5927\u591a\u6570PETases\u3002\u901a\u8fc7\u4e24\u8f6e\u7b5b\u9009\uff0c\u7814\u7a76\u8005\u53d1\u73b0\u4e86\u4e09\u4e2a\u5177\u6709\u9ad8PET\u964d\u89e3\u6f5c\u529b\u7684\u9176\u7cfb\u8c31\uff08C3\u3001C25\u548cC158\uff09\uff0c\u5e76\u4ece\u4e2d\u8bc6\u522b\u51fa\u4e24\u79cd\u5177\u6709\u9ad8\u6d3b\u6027\u7684PET\u964d\u89e3\u9176\uff1aMipa-P\u548cKubu-P\u3002\u8fd9\u4e9b\u9176\u5728PET\u5206\u89e3\u901f\u5ea6\u548c\u8010\u4e45\u6027\u65b9\u9762\u8868\u73b0\u51fa\u8272\u3002\u7814\u7a76\u8005\u5bf9Mipa-P\u548cKubu-P\u8fdb\u884c\u4e86\u7406\u6027\u5de5\u7a0b\u6539\u9020\uff0c\u751f\u6210\u4e86Mipa-PM19\u548cKubu-PM12\u53d8\u4f53\u3002\u7279\u522b\u662fKubu-PM12\uff0c\u5728\u9ad8\u5e95\u7269\u8d1f\u8377\u548c\u5de5\u4e1a\u6761\u4ef6\u4e0b\u7684PET\u964d\u89e3\u6027\u80fd\u8d85\u8fc7\u4e86\u73b0\u6709\u7684\u5de5\u7a0b\u57fa\u51c6\u9176\u3002<\/p>\n

\"\"<\/p>\n

\u56fe<\/strong>1 PETase<\/strong>\u5019\u9009\u7269\u7684\u666f\u89c2\u5206\u6790\u548c\u5206\u6790\u7a0b\u5e8f\u3002\u4f7f\u7528\u6307\u5b9a\u641c\u7d22\u8303\u56f4\u5185\u7684\u79cd\u5b50\u5e8f\u5217\u51c6\u5907\u916f\u57fa\u5851\u6599\u6c34\u89e3\u9176\uff08<\/strong>EPH<\/strong>\uff09\u5e93\u3002\u666f\u89c2\u65b9\u6cd5\u5982\u4e0b\u8fdb\u884c\uff1a\u6210\u5bf9\u8ddd\u79bb\u8ba1\u7b97\u3001\u90bb\u57df\u5206\u6790\u548c\u5e03\u5c40\u3002\u5206\u522b\u4f7f\u7528\u5206\u5c42\u548c\u805a\u7c7b\u62bd\u6837\u5728\u56fe\u4e0a\u8fdb\u884c\u521d\u7b5b\u548c\u590d\u7b5b\uff0c\u4ee5\u8ffd\u8e2a\u8be5\u86cb\u767d\u5bb6\u65cf\u7684\u9002\u5e94\u5ea6\u666f\u89c2\u3002<\/strong>TPA<\/strong>\uff0c\u5bf9\u82ef\u4e8c\u7532\u9178\uff1b<\/strong>MHET<\/strong>\uff0c\u5355<\/strong>(2-<\/strong>\u7f9f\u4e59\u57fa<\/strong>)<\/strong>\u5bf9\u82ef\u4e8c\u7532\u9178\uff1b<\/strong>BHET<\/strong>\uff0c\u53cc<\/strong>(2-<\/strong>\u7f9f\u4e59\u57fa<\/strong>)<\/strong>\u5bf9\u82ef\u4e8c\u7532\u9178\u3002<\/strong>\u00a9 2025 Science<\/strong><\/p>\n

\u672c\u6587\u91c7\u7528\u4e86\u4e00\u79cd\u57fa\u4e8e\u81ea\u7136\u5e8f\u5217\u805a\u7c7b\u6846\u67b6\u7684\u666f\u89c2\u5206\u6790\u65b9\u6cd5\u6765\u8bc4\u4f30\u548c\u7b5b\u9009\u5177\u6709PET\u964d\u89e3\u80fd\u529b\u7684\u9176\u3002\u9996\u5148\uff0c\u7814\u7a76\u8005\u4eceGenBank\u6570\u636e\u5e93\u4e2d\u68c0\u7d22\u4e8625,418\u4e2a\u975e\u5197\u4f59\u5e8f\u5217\uff0c\u901a\u8fc7PSI-BLAST\u641c\u7d22\u4f7f\u7528\u79cd\u5b50\u5e8f\u5217\u6784\u5efa\u521d\u59cb\u5e8f\u5217\u5e93\u3002\u63a5\u7740\uff0c\u5229\u7528Wilbur\u548cLipman\u7684\u8ddd\u79bb\u77e9\u9635\u65b9\u6cd5\u8fdb\u884c\u6210\u5bf9\u8ddd\u79bb\u8ba1\u7b97\uff0c\u5e76\u901a\u8fc7\u90bb\u57df\u5206\u6790\u6a21\u5757\u63a7\u5236\u7f51\u7edc\u7684\u4e25\u683c\u6027\uff0c\u751f\u6210\u4e00\u4e2a\u5305\u542b170\u4e2a\u805a\u7c7b\u548c1894\u4e2a\u8282\u70b9\u7684\u8bed\u4e49\u7f51\u7edc\u3002\u7136\u540e\uff0c\u4f7f\u7528Cytoscape\u8f6f\u4ef6\u7684Prefuse force-directed-cl\u5e03\u5c40\u7b97\u6cd5\u5c06\u5e8f\u5217\u6620\u5c04\u5230\u4e8c\u7ef4\u62d3\u6251\u7ed3\u6784\u4e2d\uff0c\u4ee5\u4fbf\u5168\u9762\u5c55\u793a\u5e8f\u5217\u4e4b\u95f4\u7684\u5173\u7cfb\u3002\u6700\u540e\uff0c\u901a\u8fc7\u5206\u5c42\u62bd\u6837\u548c\u805a\u7c7b\u62bd\u6837\u65b9\u6cd5\uff0c\u4ece\u6784\u5efa\u7684\u666f\u89c2\u4e2d\u9009\u62e9\u4ee3\u8868\u6027\u8282\u70b9\u8fdb\u884c\u5b9e\u9a8c\u9a8c\u8bc1\uff0c\u4ee5\u8bc4\u4f30\u5176PET\u964d\u89e3\u6d3b\u6027\u548c\u70ed\u7a33\u5b9a\u6027\u3002\u8fd9\u79cd\u65b9\u6cd5\u4e3a\u7cfb\u7edf\u8bc4\u4f30\u5927\u91cf\u9176\u7684\u9002\u5e94\u5ea6\u63d0\u4f9b\u4e86\u6709\u6548\u7684\u5de5\u5177\u548c\u6846\u67b6\uff0c\u6709\u52a9\u4e8e\u53d1\u73b0\u548c\u5de5\u7a0b\u5316\u5177\u6709\u5de5\u4e1a\u5e94\u7528\u6f5c\u529b\u7684PET\u964d\u89e3\u9176\u3002<\/p>\n

\"\"<\/p>\n

\u56fe<\/strong>2 PETase<\/strong>\u5019\u9009\u7269\u7684\u666f\u89c2\u5206\u6790\u3002\u6bcf\u4e2a\u8282\u70b9\u4ee3\u8868\u4e00\u4e2a\u7279\u5b9a\u7684\u5929\u7136\u5e8f\u5217\uff08\u8282\u70b9\u603b\u6570\u4e3a<\/strong>1894<\/strong>\uff09\u3002\u6839\u636e\u5728<\/strong>30<\/strong>\u00b0<\/strong>C<\/strong>\u4e0b\u5e73\u5747<\/strong>PET<\/strong>\u964d\u89e3\u6d3b\u6027\u5bf9\u6d4b\u8bd5\u8282\u70b9\u8fdb\u884c\u7740\u8272\uff0c\u8fb9\u7f18\u4ee5\u6d45\u7070\u8272\u663e\u793a\u3002\u4e09\u4e2a\u672a\u63a2\u7d22\u7684\u805a\u7c7b\u7528\u7eff\u8272\u65d7\u5e1c\u7a81\u51fa\u663e\u793a\uff0c\u4e24\u4e2a\u5f3a\u6548<\/strong>PETases Mipa-P<\/strong>\u548c<\/strong>Kubu-P<\/strong>\u7528\u7bad\u5934\u6307\u793a\u5e76\u7528\u8282\u70b9\u7f16\u53f7\u6807\u8bb0\u3002\u57fa\u51c6\u8282\u70b9\u7528\u7bad\u5934\u6307\u793a\u5e76\u76f8\u5e94\u6807\u8bb0\u3002<\/strong>\u00a9 2025 Science<\/strong><\/p>\n

\"\"<\/p>\n

\u56fe<\/strong>3 PETase<\/strong>\u7b5b\u9009\u3002<\/strong>(A) <\/strong>\u4f7f\u7528\u5206\u5c42\u62bd\u6837\u8fdb\u884c<\/strong>PETase<\/strong>\u521d\u7b5b\u3002\u4ece<\/strong>PET<\/strong>\u91ca\u653e\u53ef\u68c0\u6d4b\u5355\u4f53\u7684\u8282\u70b9\u548c\u6d3b\u6027\u53ef\u5ffd\u7565\u7684\u8282\u70b9\u5206\u522b\u7528\u7070\u8272\u548c\u9ed1\u8272\u5706\u5708\u8868\u793a\u3002\u6d3b\u52a8\u6570\u636e\u7684\u6807\u51c6\u5dee\uff08<\/strong>SD<\/strong>\uff09\u4ee5\u6761\u5f62\u56fe\u8868\u793a\u3002\u7194\u70b9\uff08<\/strong>Tm<\/sub><\/strong>\uff09\u503c\u7528\u5706\u5708\u8868\u793a\uff0c\u4e0e\u76f8\u5e94\u7684<\/strong>Boltzmann Tm<\/sub><\/strong>\u503c\u7528\u6761\u5f62\u56fe\u8fde\u63a5\u8868\u793a\u3002<\/strong>IsPETase<\/strong>\u3001<\/strong>LCC<\/strong>\u548c\u4e09\u4e2a\u672a\u63a2\u7d22\u805a\u7c7b\u4e2d\u7684\u8282\u70b9\u6309\u56fe\u4e2d\u6240\u793a\u7684\u989c\u8272\u65b9\u6848\u7740\u8272\u3002<\/strong>(B) <\/strong>\u4f7f\u7528\u805a\u7c7b\u62bd\u6837\u8fdb\u884c<\/strong>PETase<\/strong>\u590d\u7b5b\u3002<\/strong>C3<\/strong>\u3001<\/strong>C25<\/strong>\u548c<\/strong>C158<\/strong>\u4e2d\u7684\u8282\u70b9\u5728\u6d3b\u6027<\/strong>-Tm<\/sub><\/strong>\u56fe\u4e0a\u663e\u793a\u3002\u516b\u4e2a\u5408\u683c\u8282\u70b9\u88ab\u6807\u8bb0\uff0c\u521d\u7b5b\u4e2d\u7684\u4ee3\u8868\u6027\u8282\u70b9\u901a\u8fc7\u6807\u8bb0\u5f62\u72b6\u548c\u5728\u6807\u7b7e\u4e2d\u6dfb\u52a0\u661f\u53f7\u6765\u533a\u5206\u3002\u9634\u5f71\u4ee3\u8868\u8282\u70b9\u4e4b\u95f4\u7684\u8fb9\u7f18\u8fde\u63a5\u3002<\/strong>IsPETase<\/strong>\u548c<\/strong>LCC<\/strong>\u4e4b\u95f4\u7684\u8fde\u63a5\u7ebf\u7528\u865a\u7ebf\u8868\u793a\u3002<\/strong>(C) <\/strong>\u4e09\u4e2a\u672a\u63a2\u7d22\u805a\u7c7b\u7684\u7f51\u7edc\u3002\u56fe\u7684\u7ed8\u5236\u65b9\u5f0f\u4e0e\u56fe<\/strong>2<\/strong>\u76f8\u540c\u3002\u5408\u683c\u8282\u70b9\u7528\u5b9e\u7ebf\u7bad\u5934\u6307\u793a\uff0c\u521d\u7b5b\u4e2d\u672a\u88ab\u9009\u4e3a\u5408\u683c\u7684\u8282\u70b9\u7528\u865a\u7ebf\u7bad\u5934\u6307\u793a\u3002<\/strong>\u00a9 2025 Science<\/strong><\/p>\n

\"\"<\/p>\n

\u56fe<\/strong>4 Mipa-P<\/strong>\u548c<\/strong>Kubu-P<\/strong>\u4e0e\u57fa\u51c6\u7269\u7684\u6bd4\u8f83\u3002<\/strong>(A) Mipa-P<\/strong>\u548c<\/strong>Kubu-P<\/strong>\u7684\u521d\u59cb\u901f\u7387\u548c\u964d\u89e3\u7a0b\u5ea6\u4e0e\u57fa\u51c6\u7269\u8fdb\u884c\u6bd4\u8f83\u3002\u5728<\/strong>30<\/strong>\u00b0\u81f3<\/strong>70<\/strong>\u00b0<\/strong>C<\/strong>\u7684\u6e29\u5ea6\u8303\u56f4\u5185\u6d4b\u91cf\u9176\u7684\u65f6\u95f4\u8fc7\u7a0b\u6d3b\u6027\u3002\u521d\u59cb\u901f\u7387\u5bf9\u5e94\u4e8e<\/strong>3<\/strong>\u5c0f\u65f6\u7684\u8868\u89c2\u901f\u7387\uff0c\u5e76\u7528\u7070\u8272\u865a\u7ebf\u53ca\u5176\u503c\u8868\u793a\u3002\u4ece\u975e\u7ebf\u6027\u62df\u5408\u4f7f\u7528\u56db\u70b9\u903b\u8f91\u6a21\u578b\u5bfc\u51fa\u7684<\/strong>1<\/strong>\u5c0f\u65f6\u521d\u59cb\u901f\u7387\u503c\u4e5f\u5728\u62ec\u53f7\u4e2d\u6807\u8bb0\uff08\u661f\u53f7\u8868\u793a\u975e\u52a0\u6743\u62df\u5408\uff0c\u56e0\u4e3a\u52a0\u6743\u6a21\u578b\u672a\u6536\u655b\uff09\u3002<\/strong> (B) <\/strong>\u9176\u7684\u521d\u59cb\u901f\u7387\uff08<\/strong>3<\/strong>\u5c0f\u65f6\uff09\u548c\u964d\u89e3\uff08<\/strong>Depol<\/strong>\u3002\uff09\u7a0b\u5ea6\uff08<\/strong>168<\/strong>\u5c0f\u65f6\uff09\u6cbf\u6e29\u5ea6\u53d8\u5316\u3002\u9176\u7684\u521d\u59cb\u901f\u7387\u548c\u964d\u89e3\u7a0b\u5ea6\u6392\u540d\u6cbf\u6e29\u5ea6\u53d8\u5316\u7684\u96f7\u8fbe\u56fe\u4e5f\u663e\u793a\u51fa\u6765\u3002\u4ece<\/strong>1<\/strong>\u5230<\/strong>5<\/strong>\u7684\u6392\u540d\u7528\u6307\u5411\u4e2d\u5fc3\u7684\u8f74\u8868\u793a\u3002<\/strong>\u00a9 2025 Science<\/strong><\/p>\n

\"\"<\/p>\n

\u56fe<\/strong>5 Mipa-PM19<\/strong>\u548c<\/strong>Kubu-PM12<\/strong>\u7684\u6027\u80fd\u3002<\/strong>(A) Mipa-PM19<\/strong>\u548c<\/strong>Kubu-PM12<\/strong>\u7684\u6676\u4f53\u7ed3\u6784\u3002\u7ed3\u6784\u4ee5\u7070\u8272\u5361\u901a\u6a21\u578b\u663e\u793a\uff0c\u50ac\u5316\u4e09\u8054\u4f53\u7528\u9ec4\u8272\u7403\u4f53\u6a21\u578b\u7a81\u51fa\u663e\u793a\u3002\u76f8\u5bf9\u4e8e\u8d77\u59cb\u86cb\u767d\u5e8f\u5217\u7684\u7a81\u53d8\u7528\u6d0b\u7ea2\u8272\u68d2\u6a21\u578b\u533a\u5206\u5e76\u6807\u8bb0\u3002<\/strong>(B) Mipa-PM19<\/strong>\u548c<\/strong>Kubu-PM12<\/strong>\u4e0e\u5176\u91ce\u751f\u578b\u5bf9\u7167\u7269\u7684\u6bd4\u8f83\u3002\u94fe\u63a5\u5706\u5708\u6216\u4ea4\u53c9\u56fe\u8868\u793a\u901a\u8fc7\u5355\u4f53\u91ca\u653e\uff08<\/strong>HPLC<\/strong>\u91c7\u6837\uff09\u6216\u916f\u952e\u65ad\u88c2\uff08<\/strong>NaOH<\/strong>\u6d88\u8017\uff09\u8ba1\u7b97\u7684\u964d\u89e3\u7a0b\u5ea6\u3002<\/strong>(C<\/strong>\u548c<\/strong>D) <\/strong>\u9176\u5728\u9ad8\u5e95\u7269\u8d1f\u8377\u6761\u4ef6\u4e0b\u7684\u964d\u89e3\u6027\u80fd\u3002\u4ee5<\/strong>20%<\/strong>\uff08<\/strong>C<\/strong>\uff09\u548c<\/strong>30%<\/strong>\uff08<\/strong>D<\/strong>\uff09\u7684<\/strong>PET<\/strong>\u8d1f\u8377\u8fdb\u884c\u7684\u751f\u7269\u53cd\u5e94\u5668\u64cd\u4f5c\u5982\u56fe<\/strong>5B<\/strong>\u6240\u793a\u7ed8\u5236\u3002\u6bcf\u4e2a\u53cd\u5e94\u7684\u6700\u7ec8\u91cd\u91cf\u635f\u5931\u4e5f\u4ee5\u767d\u8272\u5706\u5708\u663e\u793a\u3002<\/strong>\u00a9 2025 Science<\/strong><\/p>\n

Mipa-P\u548cKubu-P\u662f\u4e24\u79cd\u5177\u6709PET\u6c34\u89e3\u6d3b\u6027\u7684\u9176\uff0c\u5206\u522b\u6765\u6e90\u4e8eMicromonospora pattaloongensis\u548cKutzneria buriramensis\u3002\u901a\u8fc7\u5bf9\u8fd9\u4e24\u79cd\u9176\u8fdb\u884c\u7406\u6027\u8bbe\u8ba1\u548c\u7a81\u53d8\u5de5\u7a0b\uff0c\u7814\u7a76\u4eba\u5458\u5f00\u53d1\u51fa\u4e86Mipa-PM19\u548cKubu-PM12\u8fd9\u4e24\u4e2a\u53d8\u4f53\u3002Mipa-PM19\u548cKubu-PM12\u5728\u70ed\u7a33\u5b9a\u6027\u65b9\u9762\u663e\u8457\u63d0\u9ad8\uff0cMipa-PM19\u7684\u7194\u70b9\uff08Tm<\/sub>\uff09\u8fbe\u5230\u4e8692.4\u00b0C\uff0c\u6bd4\u91ce\u751f\u578b\u63d0\u9ad8\u4e8624.1\u00b0C\uff0c\u800cKubu-PM12\u7684Tm<\/sub>\u751a\u81f3\u8d85\u8fc7\u4e8699.9\u00b0C\uff0c\u65e0\u6cd5\u5728\u5e38\u89c4\u6e29\u5ea6\u8303\u56f4\u5185\u6d4b\u91cf\u3002\u6b64\u5916\uff0c\u8fd9\u4e24\u79cd\u53d8\u4f53\u5728PET\u6c34\u89e3\u901f\u7387\u548c\u7a0b\u5ea6\u4e0a\u4e5f\u8868\u73b0\u51fa\u8272\uff0c\u7279\u522b\u662f\u5728\u9ad8\u6e29\u6761\u4ef6\u4e0b\uff0cMipa-PM19\u548cKubu-PM12\u7684\u521d\u59cb\u6c34\u89e3\u901f\u7387\u548c\u6700\u7ec8\u6c34\u89e3\u7a0b\u5ea6\u90fd\u9ad8\u4e8e\u5176\u91ce\u751f\u578b\u7248\u672c\u3002\u5728\u5de5\u4e1a\u76f8\u5173\u7684\u9ad8\u5e95\u7269\u8d1f\u8377\u6761\u4ef6\u4e0b\uff0cKubu-PM12\u8868\u73b0\u51fa\u5353\u8d8a\u7684\u6027\u80fd\uff0c\u80fd\u591f\u572870\u00b0C\u4e0b\u5feb\u901f\u6c34\u89e3PET\u3002<\/p>\n

\"\"<\/p>\n

\u56fe<\/strong>6<\/strong>\u4f7f\u7528<\/strong>KubuPM12<\/strong>\u8fdb\u884c\u9176\u50ac\u5316<\/strong>PET<\/strong>\u4e59\u4e8c\u9187\u89e3\u3002<\/strong>(A) <\/strong>\u9176\u50ac\u5316\u4e59\u4e8c\u9187\u89e3\u548c\u5728\u8131\u9170\u5316\u6b65\u9aa4\u7684\u53cd\u5e94\u673a\u5236\u7684\u793a\u610f\u56fe\u3002\u5728\u4e59\u4e8c\u9187\uff08<\/strong>EG<\/strong>\uff09\u6eb6\u5242\u4e0b\uff0c<\/strong>PET<\/strong>\u548c<\/strong>EG<\/strong>\u90fd\u53ef\u4ee5\u4f5c\u4e3a\u9176\u7684\u53d8\u6027\u5242\u548c\u5e95\u7269\u3002<\/strong>(B) <\/strong>\u901a\u8fc7\u5728<\/strong>EG<\/strong>\u4e2d\u9884\u5b75\u80b2\u5bf9\u4e09\u79cd\u9176\u7684\u9176\u50ac\u5316<\/strong>PET<\/strong>\u4e59\u4e8c\u9187\u89e3\u6d3b\u6027\u548c\u5931\u6d3b\u6a21\u5f0f\u8fdb\u884c\u6bd4\u8f83\u3002<\/strong>1<\/strong>\u5929\u53cd\u5e94\u540e<\/strong>BHET<\/strong>\u3001\u5355<\/strong>(2-<\/strong>\u7f9f\u4e59\u57fa<\/strong>)<\/strong>\u5bf9\u82ef\u4e8c\u7532\u9178\uff08<\/strong>MHET<\/strong>\uff09\u548c\u53ef\u5ffd\u7565\u7684\u5bf9\u82ef\u4e8c\u7532\u9178\uff08<\/strong>TPA<\/strong>\uff09\u7684\u6469\u5c14\u5206\u6570\u4ee5\u751c\u751c\u5708\u56fe\u663e\u793a\u3002<\/strong>(C) <\/strong>\u5728\u751f\u7269\u53cd\u5e94\u5668\u89c4\u6a21\u4e0a\u4f7f\u7528<\/strong>Mipa-PM19<\/strong>\u548c<\/strong>Kubu-PM12<\/strong>\u8fdb\u884c\u9176\u50ac\u5316<\/strong>PET<\/strong>\u4e59\u4e8c\u9187\u89e3\u3002\u6e29\u5ea6\u6761\u4ef6\u5982\uff08<\/strong>B<\/strong>\uff09\u4e2d\u6807\u8bb0\u3002<\/strong>\u00a9 2025 Science<\/strong><\/p>\n

\u8be5\u7814\u7a76\u7b5b\u9009\u51fa\u4e24\u79cd\u5177\u6709\u9ad8\u6d3b\u6027\u7684PET\u964d\u89e3\u9176\uff1aMipa-P\u548cKubu-P\uff0c\u5bf9Mipa-P\u548cKubu-P\u8fdb\u884c\u4e86\u7406\u6027\u5de5\u7a0b\u6539\u9020\uff0c\u751f\u6210\u4e86Mipa-PM19\u548cKubu-PM12\u53d8\u4f53\uff0cKubu-PM12\u5728\u5de5\u4e1a\u6761\u4ef6\u4e0b\u7684\u8868\u73b0\u5c24\u4e3a\u7a81\u51fa\uff0c\u80fd\u591f\u5728\u9ad8\u5e95\u7269\u8d1f\u8377\u548c\u4e59\u4e8c\u9187\u4f5c\u4e3a\u6eb6\u5242\u7684\u6761\u4ef6\u4e0b\u8fdb\u884cPET\u7684\u9176\u50ac\u5316\u6c34\u89e3\uff0c\u751f\u6210\u53cc(2-\u7f9f\u4e59\u57fa)\u5bf9\u82ef\u4e8c\u7532\u9178\u916f\uff08BHET\uff09\u4f5c\u4e3a\u4e3b\u8981\u4ea7\u7269\uff0c\u6d53\u5ea6\u53ef\u8fbe\u6570\u5341\u6beb\u6469\u5c14\u3002\u4ee5\u201cLandscape profiling of PET depolymerases using a natural sequence cluster framework<\/strong>\u201d\u4e3a\u9898\u53d1\u8868\u5728\u56fd\u9645\u9876\u7ea7\u671f\u520aScience<\/strong>\u4e0a\uff0c\u5f15\u8d77\u4e86\u76f8\u5173\u9886\u57df\u7814\u7a76\u4eba\u5458\u70ed\u8bae\u3002<\/p>\n

\"\"<\/strong><\/p>\n

\u4e09\u3001\u3010\u79d1\u5b66\u542f\u8fea\u3011<\/strong><\/p>\n

\u7efc\u4e0a\u6240\u8ff0\uff0c\u672c\u6587\u901a\u8fc7\u5f00\u53d1\u4e00\u79cd\u57fa\u4e8e\u81ea\u7136\u5e8f\u5217\u805a\u7c7b\u6846\u67b6\u7684\u666f\u89c2\u5206\u6790\u65b9\u6cd5\uff0c\u7cfb\u7edf\u5730\u8bc4\u4f30\u548c\u7b5b\u9009\u4e86\u5177\u6709\u805a\u5bf9\u82ef\u4e8c\u7532\u9178\u4e59\u4e8c\u9187\u916f\uff08PET\uff09\u964d\u89e3\u80fd\u529b\u7684\u9176\u3002\u7814\u7a76\u8005\u6784\u5efa\u4e86\u4e00\u4e2a\u5305\u542b170\u4e2a\u805a\u7c7b\u548c1894\u4e2a\u8282\u70b9\u7684\u8bed\u4e49\u7f51\u7edc\uff0c\u6db5\u76d6\u4e86\u5df2\u77e5\u7684\u5927\u591a\u6570PET\u964d\u89e3\u9176\u3002\u901a\u8fc7\u4e24\u8f6e\u7b5b\u9009\uff0c\u53d1\u73b0\u4e86\u4e09\u4e2a\u5177\u6709\u9ad8PET\u964d\u89e3\u6f5c\u529b\u7684\u9176\u7cfb\u8c31\uff08C3\u3001C25\u548cC158\uff09\uff0c\u5e76\u4ece\u4e2d\u8bc6\u522b\u51fa\u4e24\u79cd\u5177\u6709\u9ad8\u6d3b\u6027\u7684PET\u964d\u89e3\u9176\uff1aMipa-P\u548cKubu-P\u3002\u8fdb\u4e00\u6b65\u7684\u7406\u6027\u5de5\u7a0b\u6539\u9020\u751f\u6210\u4e86Mipa-PM19\u548cKubu-PM12\u53d8\u4f53\uff0c\u5176\u4e2dKubu-PM12\u5728\u9ad8\u5e95\u7269\u8d1f\u8377\u548c\u5de5\u4e1a\u6761\u4ef6\u4e0b\u7684PET\u964d\u89e3\u6027\u80fd\u8d85\u8fc7\u4e86\u73b0\u6709\u7684\u5de5\u7a0b\u57fa\u51c6\u9176\u3002\u8fd9\u4e9b\u53d1\u73b0\u4e0d\u4ec5\u4e3aPET\u7684\u751f\u7269\u964d\u89e3\u63d0\u4f9b\u4e86\u65b0\u7684\u9176\u8d44\u6e90\uff0c\u8fd8\u4e3a\u5de5\u4e1a\u5e94\u7528\u4e2dPET\u7684\u56de\u6536\u5229\u7528\u63d0\u4f9b\u4e86\u6f5c\u5728\u7684\u6280\u672f\u652f\u6301\u3002<\/p>\n

 <\/p>\n

\u539f\u6587\u8be6\u60c5<\/strong>\uff1aSeo, H., Hong, H., Park, J., Lee, S. H., Ki, D., Ryu, A., Sagong, H.-Y., & Kim, K.-J. (2025). Landscape profiling of PET depolymerases using a natural sequence cluster framework. Science, 387(6701), eadp5637. https:\/\/doi.org\/10.1126\/science.adp5637<\/p>\n

 <\/p>\n

\u672c\u6587\u7531\u666f\u884c\u64b0\u7a3f<\/strong><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u4e00\u3001\u3010\u79d1\u5b66\u80cc\u666f\u3011\u00a0 \u805a\u5bf9\u82ef\u4e8c\u7532\u9178\u4e59\u4e8c\u9187\u916f\uff08PET\uff09\u662f\u4e00\u79cd\u5e7f\u6cdb\u4f7f\u7528\u7684\u5851\u6599\u6750\u6599\uff0c\u56e0\u5176\u8010\u4e45\u6027\u548c\u5e7f\u6cdb\u7684\u5e94\u7528\u800c\u96be\u4ee5\u81ea\u7136\u964d\u89e3\uff0c\u5bfc\u81f4\u4e25\u91cd\u7684\u73af\u5883\u6c61\u67d3\u95ee\u9898\u3002\u8fd1\u5e74\u6765\uff0c\u79d1\u5b66\u5bb6\u4eec\u81f4\u529b\u4e8e…<\/p>\n","protected":false},"author":36004,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[67],"tags":[],"class_list":["post-264377","post","type-post","status-publish","format-standard","hentry","category-zhoubao"],"_links":{"self":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/264377"}],"collection":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/users\/36004"}],"replies":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=264377"}],"version-history":[{"count":1,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/264377\/revisions"}],"predecessor-version":[{"id":264385,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/264377\/revisions\/264385"}],"wp:attachment":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=264377"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=264377"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=264377"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}