{"id":26376,"date":"2016-07-13T08:00:38","date_gmt":"2016-07-13T00:00:38","guid":{"rendered":"\/\/m.iemloyee.com\/?p=26376"},"modified":"2016-09-28T13:41:59","modified_gmt":"2016-09-28T05:41:59","slug":"%e5%ad%a6%e6%9c%af%e5%b9%b2%e8%b4%a7-%e8%8d%a7%e5%85%89%ef%bc%8c%e4%bd%a0%e6%89%80%e7%9f%a5%e9%81%93%e4%b8%8e%e6%89%80%e4%b8%8d%e7%9f%a5%e9%81%93%e7%9a%84%ef%bc%88%e9%99%84%e5%b8%a6%e8%8d%a7","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=26376","title":{"rendered":"\u5b66\u672f\u5e72\u8d27 | \u8367\u5149\uff0c\u4f60\u6240\u77e5\u9053\u4e0e\u6240\u4e0d\u77e5\u9053\u7684\uff08\u9644\u5e26\u8367\u5149\u6d4b\u8bd5\u5fc5\u5907\u5c0f\u77e5\u8bc6\uff09"},"content":{"rendered":"<p><strong><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-26378 size-full\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2016\/07\/1.jpeg\" alt=\"1\" width=\"600\" height=\"305\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2016\/07\/1.jpeg 600w, 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src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2016\/07\/3\u57fa\u672c\u539f\u7406.jpg\" alt=\"3\u57fa\u672c\u539f\u7406\" width=\"700\" height=\"1050\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2016\/07\/3\u57fa\u672c\u539f\u7406.jpg 700w, \/\/m.iemloyee.com\/wp-content\/uploads\/2016\/07\/3\u57fa\u672c\u539f\u7406-200x300.jpg 200w, \/\/m.iemloyee.com\/wp-content\/uploads\/2016\/07\/3\u57fa\u672c\u539f\u7406-683x1024.jpg 683w\" sizes=\"(max-width: 700px) 100vw, 700px\" \/><\/p>\n<h2><span style=\"color: #ff0000;\">\u8367\u5149\u7684\u57fa\u672c\u5e94\u7528<\/span><\/h2>\n<h3><strong>\u751f\u7269\u63a2\u9488<\/strong><\/h3>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-26381 size-full\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2016\/07\/4-24.jpg\" alt=\"4\" width=\"465\" height=\"465\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2016\/07\/4-24.jpg 465w, \/\/m.iemloyee.com\/wp-content\/uploads\/2016\/07\/4-24-150x150.jpg 150w, \/\/m.iemloyee.com\/wp-content\/uploads\/2016\/07\/4-24-300x300.jpg 300w\" sizes=\"(max-width: 465px) 100vw, 465px\" \/><\/p>\n<p>\u4e0a\u56fe\u663e\u793a\u7684\u662f\u901a\u8fc7\u8367\u5149\u80fd\u91cf\u5171\u632f\u8f6c\u79fb\uff08FRET\uff09\u73b0\u8c61\uff0c\u6807\u8bb0\u751f\u7269\u57fa\u56e0\u7684\u8868\u8fbe\u8fc7\u7a0b\u3002\u56fe\u4e2d\u7684\u76ee\u6807\u57fa\u56e0\u5728\u6ca1\u6709\u8868\u8fbe\u7684\u65f6\u5019\uff0c\u6807\u8bb0\u5728\u4e0d\u540cRNA\u5206\u652f\u4e0a\u7684\u4f9b\u4f53\u67d3\u6599\u548c\u53d7\u4f53\u67d3\u6599\u56e0\u4e3a\u6dec\u706d\u5242\u7684\u5b58\u5728\u4e0d\u4f1a\u53d1\u5149\uff0c\u800c\u5f53\u7ec6\u80de\u53d7\u5230\u523a\u6fc0\uff0c\u76f8\u5e94\u7684\u57fa\u56e0\u5f00\u59cb\u8868\u8fbe\u65f6\uff0c\u8f6c\u5f55\u51fa\u6765\u7684RNA\u4f1a\u4e0e\u6539\u9020\u540e\u7684RNA\u76f8\u63a5\u5904\uff0c\u8fdb\u800c\u4f7f\u5f97\u67d3\u6599\u53d1\u5149\u3002\uff08Santangelo PJ, Nix B, Tsourkas A, Bao G. 2004. Dual FRET molecularbeacons for mRNA detection in living cells. 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Shedding light onto live moleculartargets. Nature Med 9(1):123\u2013128.\uff09<\/p>\n<h3><strong>\u5355\u5206\u5b50\u68c0\u6d4b<\/strong><\/h3>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-26384 size-full\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2016\/07\/7-15.jpg\" alt=\"7\" width=\"465\" height=\"567\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2016\/07\/7-15.jpg 465w, \/\/m.iemloyee.com\/wp-content\/uploads\/2016\/07\/7-15-246x300.jpg 246w\" sizes=\"(max-width: 465px) 100vw, 465px\" \/><\/p>\n<p>\u5355\u5206\u5b50\u8367\u5149\u68c0\u6d4b\u5728\u5316\u5b66\u5206\u6790\u3001\u7eb3\u7c73\u6750\u6599\u5206\u6790\u3001\u533b\u5b66\u8bca\u65ad\u3001\u6cd5\u533b\u5206\u6790\u3001\u751f\u7269\u6750\u6599\u7814\u7a76\u7b49\u65b9\u9762\u90fd\u5177\u6709\u72ec\u7279\u7684\u5e94\u7528\u4ef7\u503c\uff0c\u5bf9\u8bb8\u591a\u5b66\u79d1\u9886\u57df\u7684\u53d1\u5c55\u4ea7\u751f\u4e86\u548c\u6b63\u5728\u4ea7\u751f\u7740\u6df1\u8fdc\u7684\u5f71\u54cd\u3002\u800c\u53bb\u5e74\u83b7\u5f97\u8bfa\u8d1d\u5c14\u5316\u5b66\u5956\u7684\u8d85\u5206\u8fa8\u8367\u5149\u663e\u5fae\u6280\u672f\u5728\u8be5\u9886\u57df\u6709\u7740\u5341\u5206\u91cd\u8981\u7684\u5e94\u7528\u3002<\/p>\n<h3><strong>\u7a00\u571f\u53d1\u5149\u6750\u6599<\/strong><\/h3>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-26385 size-full\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2016\/07\/8-11.jpg\" alt=\"8\" width=\"653\" height=\"467\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2016\/07\/8-11.jpg 653w, \/\/m.iemloyee.com\/wp-content\/uploads\/2016\/07\/8-11-300x215.jpg 300w\" sizes=\"(max-width: 653px) 100vw, 653px\" \/><\/p>\n<p>\u76f8\u6bd4\u4e8e\u6709\u673a\u5206\u5b50\uff0c\u8367\u5149\u7a00\u571f\u5143\u7d20\u5728\u8367\u5149\u6027\u8d28\u65b9\u9762\u5177\u6709\u5f88\u5927\u7684\u4f18\u52bf\uff0c\u5982\u8272\u5ea6\u7eaf\u9ad8\u3001\u5bff\u547d\u957f\u7b49\u4f18\u70b9\u3002\u4ece\u4e0a\u56fe\u67d0\u542b\u7a00\u571f\u94d5\u5143\u7d20\u7684\u6837\u54c1\u7684\u8367\u5149\u53d1\u5c04\u5149\u8c31\u4e2d\u53ef\u4ee5\u770b\u51fa\uff0c\u8be5\u6837\u54c1\u7684\u8367\u5149\u53d1\u5c04\u5cf0\u8f83\u7a84\uff0c\u8fd9\u6b63\u662f\u5176\u8272\u7eaf\u5ea6\u9ad8\u7684\u4f53\u73b0\u3002\u800c\u5408\u7406\u8bbe\u8ba1\u7a00\u571f\u5143\u7d20\u7684\u7ec4\u6210\uff0c\u8fd8\u53ef\u4ee5\u5408\u6210\u51fa\u4e0a\u8f6c\u5316\u53d1\u5149\u6750\u6599\u3002\u4e0a\u8f6c\u6362\u6750\u6599\u662f\u4e00\u79cd\u7ea2\u5916\u5149\u6fc0\u53d1\u4e0b\u80fd\u53d1\u51fa\u53ef\u89c1\u5149\u7684\u53d1\u5149\u6750\u6599,\u5373\u5c06\u7ea2\u5916\u5149\u8f6c\u6362\u6210\u53ef\u89c1\u5149\u7684\u6750\u6599\uff0c\u8fd9\u4e5f\u662f\u662f\u6240\u8c13\u591c\u89c6\u955c\u7684\u57fa\u672c\u539f\u7406\u3002\uff08Tang, S.; Babai, A.; Mudring, A. V.2008. Europium-based ionic liquids as luminescent soft materials. Angew. Chem., Int. Ed 47 (40)\uff1a7631-7634.\uff09<\/p>\n<h2><span style=\"color: #ff0000;\">\u8367\u5149\u6d4b\u8bd5\u5fc5\u5907\u5c0f\u77e5\u8bc6<\/span><\/h2>\n<ul>\n<li>\u5bf9\u4e8e\u672a\u77e5\u6837\u54c1\uff0c\u5efa\u8bae\u5927\u5bb6\u5148\u6d4b\u4e00\u4e0b\u6837\u54c1\u7684\u5438\u6536\u8c31\uff0c\u5e76\u4ece\u4e2d\u627e\u51fa\u611f\u5174\u8da3\u7684\u5438\u6536\u5cf0\u548c\u7279\u6027\uff0c\u5728\u8367\u5149\u6d4b\u8bd5\uff08\u6fc0\u53d1\u548c\u53d1\u5c04\u5149\u8c31\u6d4b\u8bd5\uff09\u65f6\u4ee5\u4fbf\u53c2\u8003\u3002<\/li>\n<li><strong>\u53d1\u5c04\u5149\u8c31<\/strong>\uff1a\u6839\u636e\u5438\u6536\u8c31\u4e2d\u611f\u5174\u8da3\u7684\u5cf0\u786e\u5b9a\u6fc0\u53d1\u6ce2\u957f\uff0c\u9009\u62e9\u5408\u9002\u7684<strong>\u53d1\u5c04\u8c31\u6ce2\u957f\u8303\u56f4<\/strong>\u3001\u6ee4\u5149\u7247\u3001\u5149\u8def\u72ed\u7f1d\u3001\u626b\u63cf\u901f\u5ea6\u7b49\u8fdb\u884c\u53d1\u5c04\u8c31\u7684\u626b\u63cf\u3002<\/li>\n<li><strong>\u6fc0\u53d1\u5149\u8c31<\/strong>\uff1a\u6839\u636e\u53d1\u5c04\u8c31\u4e2d\u611f\u5174\u8da3\u7684\u5cf0\u786e\u5b9a\u53d1\u5c04\u6ce2\u957f\uff0c\u9009\u62e9\u5408\u9002\u7684<strong>\u6fc0\u53d1\u8c31\u6ce2\u957f\u8303\u56f4<\/strong>\u3001\u6ee4\u5149\u7247\u3001\u5149\u8def\u72ed\u7f1d\u3001\u626b\u63cf\u901f\u5ea6\u7b49\u8fdb\u884c\u6fc0\u53d1\u8c31\u7684\u626b\u63cf\u3002<\/li>\n<li><strong>\u6ee4\u5149\u7247\u7684\u9009\u62e9<\/strong>\uff1a\u53d1\u5c04\u8c31\u626b\u63cf\u8fc7\u7a0b\u4e2d\uff0c\u901a\u5e38\u9009\u62e9\u6bd4\u6fc0\u53d1\u6ce2\u957f\u957f\uff0c\u6bd4\u53d1\u5c04\u8c31\u6700\u77ed\u6ce2\u957f\u77ed\u7684\u957f\u6ce2\u901a\u578b\u622a\u6b62\u6ee4\u5149\u7247\u3002\u6fc0\u53d1\u8c31\u626b\u63cf\u8fc7\u7a0b\u4e2d\uff0c\u901a\u5e38\u9009\u62e9\u6bd4\u53d1\u5c04\u6ce2\u957f\u77ed\uff0c\u6bd4\u6fc0\u53d1\u8c31\u6700\u957f\u6ce2\u957f\u957f\u7684\u957f\u6ce2\u901a\u578b\u622a\u6b62\u6ee4\u5149\u7247\u3002<\/li>\n<li><strong>\u72ed\u7f1d\u7684\u9009\u62e9\uff1a<\/strong>\u5982\u679c\u5149\u8def\u72ed\u7f1d\u592a\u5927\uff0c\u8367\u5149\u4fe1\u53f7\u592a\u5f3a\uff0c\u5bb9\u6613\u8d85\u51fa\u4eea\u5668\u68c0\u6d4b\u8303\u56f4\uff0c\u635f\u4f24\u4eea\u5668\uff1b\u5982\u679c\u72ed\u7f1d\u5f00\u7684\u592a\u5c0f\uff0c\u8367\u5149\u4fe1\u53f7\u53c8\u592a\u5f31\uff0c\u68c0\u6d4b\u6bd4\u8f83\u56f0\u96be\uff0c\u6240\u4ee5\u8981\u9009\u62e9\u5927\u5c0f\u5408\u9002\u7684\u72ed\u7f1d\u3002<\/li>\n<li><strong>\u626b\u63cf\u901f\u5ea6\u7684\u9009\u62e9\uff1a<\/strong>\u5982\u679c\u626b\u63cf\u592a\u5feb\uff0c\u5bb9\u6613\u8df3\u5cf0\uff0c\u5ffd\u7565\u7279\u5f81\u6027\u7684\u5cf0\u4fe1\u53f7\uff1b\u626b\u63cf\u592a\u6162\u5219\u6d6a\u8d39\u65f6\u95f4\u3002\u6240\u4ee5\u53ea\u8981\u80fd\u626b\u63cf\u5f97\u5230\u5e73\u6ed1\u7684\u5149\u8c31\u66f2\u7ebf\u5c31\u53ef\u4ee5\u4e86\u3002<\/li>\n<\/ul>\n<p>\u53c2\u8003\u8d44\u6599\uff1a<\/p>\n<p>[1] Joseph R. Lakowicz,Principles of Fluorescence Spectroscopy, Third Edition.<\/p>\n<p>[2] Santangelo PJ, Nix B, Tsourkas A, Bao G. 2004. Dual FRET molecularbeacons for mRNA detection in living cells. Nucleic Acids Res32(6):e57.<\/p>\n<p>[3] Weissleder R, Ntziachristos V. 2003. Shedding light onto live moleculartargets. Nature Med 9(1):123\u2013128.<\/p>\n<p>[4] Tang, S.; Babai, A.; Mudring, A. V.2008. Europium-based ionic liquids as luminescent soft materials. Angew. Chem., Int. Ed 47 (40)\uff1a7631-7634.<\/p>\n<p>\u672c\u6587\u7531\u6750\u6599\u4eba\u7f16\u8f91\u90e8\u4f55\u946b\u4f9b\u7a3f\uff0c\u6750\u6599\u725b\u7f16\u8f91\u6574\u7406\u3002<\/p>\n<p>\u6b22\u8fce\u4f18\u79c0\u7855\u3001\u535a\u58eb\u751f\u52a0\u5165\u6750\u6599\u4eba\u7f16\u8f91\u90e8\u5b66\u672f\u5e72\u8d27\u5c0f\u7ec4\uff0c\u4e00\u8d77\u4f20\u64ad\u5b66\u672f\u77e5\u8bc6\uff0c\u5c55\u73b0\u4f60\u7684\u624d\u534e\uff0c\u52a9\u529b\u6750\u6599\u79d1\u7814\uff0c\u4f18\u79c0\u7a3f\u4ef6\u4e00\u7ecf\u5f55\u7528\uff0c\u6211\u4eec\u4f1a\u5949\u4e0a\u7a3f\u916c\uff0c\u8d76\u5feb\u79c1\u4fe1\u7ba1\u7406\u5458\u8d1f\u8d23\u4eba\u201c\u6de1\u5e74\u534e\uff08QQ:601416321\uff09\u201d\u62a5\u540d\u5427\uff01<\/p>\n<p>\u6b22\u8fce\u5404\u5927\u8bfe\u9898\u7ec4\u5230\u6750\u6599\u4eba\u5ba3\u4f20\u79d1\u6280\u6210\u679c\u5e76\u5bf9\u6587\u732e\u8fdb\u884c\u6df1\u5165\u89e3\u8bfb\uff0c\u6295\u7a3f\u90ae\u7bb1<a href=\"mailto:tougao@cailiaoren.com\" target=\"_blank\">tougao@cailiaoren.com<\/a>\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u5f15\u5b50 \u8c08\u53ca\u8367\u5149\uff0c\u5c0f\u7f16\u7684\u8111\u6d77\u4e2d\u9996\u5148\u6d6e\u73b0\u51fa\u4e00\u4f4d\u7537\u795e\u2014\u2014\u94b1\u6c38\u5065\u5148\u751f\u3002\u56e0\u4e3a\u5176\u5728\u7eff\u8272\u8367\u5149\u86cb\u767d\u65b9\u9762\u7684\u7a81\u7834\u6027\u7814\u7a76\uff0c\u94b1\u6c38\u5065\u5148\u751f\u4e0e\u5176\u4ed6\u4e24\u4f4d\u79d1\u5b66\u5bb6\u5171\u540c\u5206\u4eab\u4e862008\u5e74\u7684\u8bfa\u8d1d\u5c14\u5316\u5b66\u5956&#8230;<\/p>\n","protected":false},"author":575,"featured_media":26378,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"class_list":["post-26376","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-ganhuo"],"_links":{"self":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/26376"}],"collection":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/users\/575"}],"replies":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26376"}],"version-history":[{"count":4,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/26376\/revisions"}],"predecessor-version":[{"id":26538,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/26376\/revisions\/26538"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/media\/26378"}],"wp:attachment":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26376"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26376"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26376"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}