{"id":255637,"date":"2023-11-06T06:52:52","date_gmt":"2023-11-05T22:52:52","guid":{"rendered":"\/\/m.iemloyee.com\/?p=255637"},"modified":"2023-11-05T22:53:02","modified_gmt":"2023-11-05T14:53:02","slug":"nature-methods%ef%bc%9a%e7%94%a8%e4%ba%8e%e6%94%b9%e5%8f%98%e4%bd%93%e6%84%9f%e7%9a%84%e6%8a%97%e7%96%b2%e5%8a%b3%e5%8f%af%e6%8b%89%e4%bc%b8%e6%b0%b4%e5%87%9d%e8%83%b6","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=255637","title":{"rendered":"Nature Methods\uff1a\u7528\u4e8e\u6539\u53d8\u4f53\u611f\u7684\u6297\u75b2\u52b3\u53ef\u62c9\u4f38\u6c34\u51dd\u80f6"},"content":{"rendered":"<p><strong>\u4e00\u3001\u3010\u5bfc\u8bfb\u3011<\/strong><\/p>\n<p>\u5149\u9057\u4f20\u5b66\u901a\u8fc7\u5bf9\u7279\u5b9a\u7ec6\u80de\u7fa4\u7684\u6d3b\u52a8\u8fdb\u884c\u7cbe\u786e\u63a7\u5236\uff0c\u6539\u53d8\u4e86\u7cfb\u7edf\u795e\u7ecf\u79d1\u5b66\u9886\u57df\u3002\u867d\u7136\u5149\u9057\u4f20\u5b66\u5df2\u88ab\u5e38\u89c4\u5e94\u7528\u4e8e\u5927\u8111\u56de\u8def\u7814\u7a76\uff0c\u4f46\u5c06\u5176\u5e94\u7528\u4e8e\u5267\u70c8\u8fd0\u52a8\u7684\u5916\u5468\u795e\u7ecf\u4ecd\u5177\u6709\u6311\u6218\u6027\u3002\u5149\u4f20\u8f93\u88c5\u7f6e\u7531\u5177\u6709GPa\u6a21\u91cf\u7684\u7845\u7ea4\u7ef4\u7b49\u521a\u6027\u6750\u6599\u7ec4\u6210\uff0c\u5728\u53cd\u590d\u53d8\u5f62\u548c\u4f4d\u79fb\u65f6\u53ef\u80fd\u4f1a\u635f\u574f\u6a21\u91cf\u5728kPa\u5230MPa\u8303\u56f4\u5185\u7684\u8f6f\u7ec4\u7ec7\u3002\u6b64\u5916\uff0c\u5c06\u521a\u6027\u88c5\u7f6e\u690d\u5165\u56db\u80a2\u53ef\u80fd\u4f1a\u59a8\u788d\u52a8\u7269\u7684\u81ea\u7136\u884c\u4e3a\u3002\u867d\u7136\u901a\u8fc7\u5fae\u52a0\u5de5\u5236\u9020\u7684\u5e26\u6709\u5fae\u578b\u53d1\u5149\u4e8c\u6781\u7ba1\u7684\u67d4\u6027\u88c5\u7f6e\u9002\u7528\u4e8e\u523a\u6fc0\u81ea\u7531\u6d3b\u52a8\u7684\u556e\u9f7f\u7c7b\u52a8\u7269\u7684\u5916\u5468\u795e\u7ecf\u548c\u810a\u9ad3\uff0c\u4f46\u5176\u590d\u6742\u7684\u8bbe\u8ba1\u7ed9\u4e13\u4e1a\u8bbe\u65bd\u4e4b\u5916\u7684\u5b9e\u65bd\u5e26\u6765\u4e86\u6311\u6218\u3002\u66f4\u503c\u5f97\u6ce8\u610f\u7684\u662f\uff0c\u8fd0\u52a8\u8fc7\u7a0b\u4e2d\u7684\u5267\u70c8\u548c\u53cd\u590d\u53d8\u5f62\u53ef\u80fd\u4f1a\u52a0\u901f\u5fae\u52a0\u5de5\u8bbe\u5907\u5185\u8f6f\u786c\u754c\u9762\u7684\u75b2\u52b3\u5931\u6548\uff0c\u5bfc\u81f4\u795e\u7ecf\u8c03\u63a7\u7684\u529f\u6548\u548c\u53ef\u9760\u6027\u968f\u7740\u65f6\u95f4\u7684\u63a8\u79fb\u800c\u964d\u4f4e\u3002<\/p>\n<p><strong>\u00a0<\/strong><strong>\u4e8c\u3001\u3010\u6210\u679c\u63a0\u5f71\u3011<\/strong><\/p>\n<p>\u8fd1\u65e5\uff0c<strong>\u7f8e\u56fd\u9ebb\u7701\u7406\u5de5\u5b66\u9662\u5218\u5fc3\u60a6\u535a\u58eb\u3001<\/strong><strong>Polina Anikeeva<\/strong><strong>\u6559\u6388\u3001\u8d75\u9009\u8d3a\u6559\u6388\u4ee5\u53ca\u9a6c\u8428\u8bf8\u585e\u5927\u5b66\u963f\u9ed8\u65af\u7279\u5206\u6821\u9976\u601d\u5706\u535a\u58eb<\/strong>\u7b49\u4eba\u5f00\u53d1\u4e86\u67d4\u8f6f\u4e14\u53ef\u62c9\u4f38\u7684\u6297\u75b2\u52b3\u6c34\u51dd\u80f6\u5149\u7ea4\uff0c\u53ef\u5728\u52a8\u7269\u6301\u7eed\u8fd0\u52a8\u65f6\u5bf9\u5176\u5916\u5468\u795e\u7ecf\u8fdb\u884c\u5149\u9057\u4f20\u8c03\u63a7\u3002\u901a\u8fc7\u5728\u6c34\u51dd\u80f6\u4e2d\u5f62\u6210\u9ad8\u5206\u5b50\u7eb3\u7c73\u6676\u57df\uff0c\u7ea4\u7ef4\u7684\u5149\u5b66\u635f\u8017\u4f4e\u81f31.07 dB cm<sup>-1<\/sup>\uff0c\u6768\u6c0f\u6a21\u91cf\u4e3a1.6 MPa\uff0c\u53ef\u62c9\u4f38200%\uff0c\u62c9\u4f3830000\u6b21\u540e\u7684\u75b2\u52b3\u5f3a\u5ea6\u4e3a1.4 MPa\u3002\u6c34\u51dd\u80f6\u7ea4\u7ef4\u53ef\u5c06\u5149\u4f20\u9012\u5230\u5750\u9aa8\u795e\u7ecf\uff0c\u5728\u4e3a\u671f6\u5468\u7684\u81ea\u4e3b\u8f66\u8f6e\u8dd1\u6b65\u8bd5\u9a8c\u4e2d\uff0c\u5149\u9057\u4f20\u6fc0\u6d3bThy1::ChR2\u5c0f\u9f20\u7684\u540e\u80a2\u808c\u8089\uff0c\u540c\u65f6\u7ecf\u5386\u53cd\u590d\u53d8\u5f62\u3002\u6b64\u5916\uff0c\u8fd9\u79cd\u7ea4\u7ef4\u8fd8\u80fd\u5728\u708e\u75c7\u6a21\u578b\u4e2d\u5bf9TRPV1::NpHR\u5c0f\u9f20\u8fdb\u884c\u4e3a\u671f8\u5468\u7684\u75bc\u75db\u8d85\u654f\u53cd\u5e94\u8fdb\u884c\u5149\u5b66\u6291\u5236\u3002\u5236\u5907\u7684\u6c34\u51dd\u80f6\u7ea4\u7ef4\u4e3a\u5916\u5468\u795e\u7ecf\u5149\u9057\u4f20\u5b66\u63d0\u4f9b\u4e86\u4e00\u79cd\u8fd0\u52a8\u9002\u5e94\u6027\u5f3a\u3001\u575a\u56fa\u8010\u7528\u7684\u89e3\u51b3\u65b9\u6848\uff0c\u6709\u52a9\u4e8e\u4f53\u611f\u7684\u7814\u7a76\u3002\u7814\u7a76\u6210\u679c\u4ee5\u9898\u4e3a\u201c<strong>Fatigue-resistant hydrogel optical fibers enable peripheral nerve optogenetics during locomotion<\/strong>\u201d\u53d1\u8868\u5728\u77e5\u540d\u671f\u520a<strong><em>Nature Methods<\/em><\/strong><strong><em>\u4e0a<\/em><\/strong>\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-255638\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2023\/11\/0-3.png\" alt=\"\" width=\"600\" height=\"350\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2023\/11\/0-3.png 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2023\/11\/0-3-300x175.png 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p><strong>\u4e09\u3001\u3010\u6838\u5fc3\u521b\u65b0\u70b9\u3011<\/strong><\/p>\n<p>1\u3001\u5f00\u53d1\u4e86\u4e00\u79cd\u67d4\u8f6f\u3001\u53ef\u53cd\u590d\u62c9\u4f38\u7684\u6c34\u51dd\u80f6\u5149\u7ea4\uff1b<\/p>\n<p>2\u3001\u5c06\u8fd9\u79cd\u67d4\u6027\u5149\u7ea4\u690d\u5165\u4f53\u5185\uff0c\u4e0d\u4ec5\u80fd\u591f\u6709\u6548\u7684\u628a\u5149\u4fe1\u53f7\u4f20\u8f93\u5230\u8fd0\u52a8\u60c5\u51b5\u590d\u6742\u7684\u5916\u5468\u795e\u7ecf\uff0c\u540c\u65f6\u4e0d\u5f71\u54cd\u5b9e\u9a8c\u52a8\u7269\u6301\u7eed\u7684\u6b63\u5e38\u8fd0\u52a8\uff1b<\/p>\n<p>3\u3001\u5728\u5b9e\u9a8c\u52a8\u7269\u6b63\u5e38\u81ea\u7136\u884c\u4e3a\u5b66\u7684\u72b6\u6001\u4e0b\uff0c\u901a\u8fc7\u5149\u9057\u4f20\u5b66\u5b9e\u73b0\u4e86\u5bf9\u5916\u5468\u795e\u7ecf\u523a\u6fc0\u548c\u6291\u5236\u3002<\/p>\n<p><strong>\u56db\u3001\u3010\u6570\u636e\u6982\u89c8\u3011<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-255639\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2023\/11\/1-2.png\" alt=\"\" width=\"600\" height=\"691\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2023\/11\/1-2.png 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2023\/11\/1-2-260x300.png 260w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p><strong>\u56fe<\/strong><strong>1\u00a0 <\/strong><strong>\u6c34\u51dd\u80f6\u5149\u7ea4\u7684\u8bbe\u8ba1\u548c\u673a\u7406<\/strong><strong> \u00a9 2023 Springer Nature<\/strong><\/p>\n<p>\uff08a\uff09\u690d\u5165\u76ae\u80a4\u4e0b\u7684\u6c34\u51dd\u80f6\u7ea4\u7ef4\u793a\u610f\u56fe\uff0c\u7528\u4e8e\u5c06\u5149\u4f20\u9012\u5230\u5916\u5468\u795e\u7ecf\uff0c\u4ee5\u53ca\u5b83\u4eec\u5728\u52a8\u7269\u8fd0\u52a8\u8fc7\u7a0b\u4e2d\u7ecf\u5386\u7684\u91cd\u590d\u62c9\u4f38\u3002<\/p>\n<p>\uff08b\uff09\u901a\u8fc7\u6c34\u51dd\u80f6\u7ea4\u7ef4\u5bf9\u5916\u5468\u795e\u7ecf\u4e2d\u8868\u8fbe\u89c6\u86cb\u767d\uff08\u4f8b\u5982ChR2\u548cNpHR\uff09\u7684\u8fd0\u52a8\u548c\u611f\u89c9\u795e\u7ecf\u5143\u7684\u5149\u9057\u4f20\u5b66\u6fc0\u6d3b\u6216\u6291\u5236\u3002<\/p>\n<p>\uff08c\uff09\u6c34\u51dd\u80f6\u7ea4\u7ef4\u793a\u610f\u56fe\uff0c\u7531\u9676\u74f7\u5149\u5b66\u5957\u5708\u4e2d\u7684\u77ed\u4e8c\u6c27\u5316\u7845\u7ea4\u7ef4\u3001\u6c34\u51dd\u80f6\u82af\u3001\u6c34\u51dd\u80f6\u5305\u5c42\u548c\u7ec4\u7ec7\u6db2\u4e2d\u7684\u6c34\u51dd\u80f6\u5957\u7ec4\u6210\u3002<\/p>\n<p>\uff08d\uff09\u6c34\u51dd\u80f6\u82af\u6750\u6599\u7c98\u9644\u5728\u77ed\u4e8c\u6c27\u5316\u7845\u7ea4\u7ef4\u4e0a\uff0c\u5b9e\u73b0\u5957\u5708\u548c\u7ea4\u7ef4\u4e4b\u95f4\u7684\u6297\u75b2\u52b3\u8fde\u63a5\u3002<\/p>\n<p>\uff08e\uff09\u6c34\u51dd\u80f6\u8896\u5e26\u5305\u88f9\u5728\u76ee\u6807\u795e\u7ecf\u5468\u56f4\uff0c\u5b9e\u73b0\u795e\u7ecf\u548c\u7ea4\u7ef4\u4e4b\u95f4\u7684\u8fd0\u52a8\u9002\u5e94\u6027\u8fde\u63a5\u3002<\/p>\n<p>\uff08f\uff09\u6c34\u51dd\u80f6\u82af\u6750\u6599\u5305\u542b\u6bd4\u5149\u6ce2\u957f\u5c0f\u5f97\u591a\u7684\u7eb3\u7c73\u6676\u7574\uff0c\u80fd\u591f\u6700\u5927\u9650\u5ea6\u5730\u51cf\u5c11\u5149\u5438\u6536\u548c\u6563\u5c04\u3002<\/p>\n<p>\uff08g\uff09\u6c34\u51dd\u80f6\u6838\u5fc3\u6750\u6599\u548c\u6c34\u51dd\u80f6\u5305\u5c42\u6750\u6599\u5728 473 nm\u548c 589 nm\u6ce2\u957f\u5904\u7684\u5f52\u4e00\u5316\u5438\u5149\u5ea6\u3002<\/p>\n<p>\uff08h\uff09\u6c34\u51dd\u80f6\u82af\u6750\u6599\u8868\u73b0\u51fa\u6bd4\u6c34\u51dd\u80f6\u5305\u5c42\u6750\u6599\u548c\u7ec4\u7ec7\u6db2\u66f4\u9ad8\u7684\u6298\u5c04\u7387\uff0c\u80fd\u591f\u6700\u5927\u9650\u5ea6\u5730\u51cf\u5c11\u82af-\u5305\u5c42\u754c\u9762\u7684\u5149\u6cc4\u6f0f\u3002<\/p>\n<p>\uff08i\uff09\u6c34\u51dd\u80f6\u82af\u548c\u5305\u5c42\u6750\u6599\u7684\u6298\u5c04\u7387\u3002<\/p>\n<p>\uff08j-k\uff09473 nm\u548c589 nm\u5904\u5f52\u4e00\u5316\u5149\u5f3a\u4e0e\u5149\u7ea4\u957f\u5ea6\u7684\u5173\u7cfb\u56fe\u3002<\/p>\n<p>\uff08l\uff09\u84dd\u5149\u901a\u8fc7\u53d8\u5f62\u7684\u6c34\u51dd\u80f6\u7ea4\u7ef4\u4f20\u8f93\u7684\u4ee3\u8868\u6027\u56fe\u50cf\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-255640\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2023\/11\/2-3.png\" alt=\"\" width=\"600\" height=\"366\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2023\/11\/2-3.png 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2023\/11\/2-3-300x183.png 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p><strong>\u56fe<\/strong><strong>2\u00a0 <\/strong><strong>\u6c34\u51dd\u80f6\u5149\u7ea4\u7684\u673a\u68b0\u575a\u56fa\u6027\u548c\u7075\u6d3b\u6027<\/strong><strong> \u00a9 2023 Springer Nature<\/strong><\/p>\n<p>\uff08a\uff09\u6c34\u51dd\u80f6\u7ea4\u7ef4\u5728\u5355\u5faa\u73af\u62c9\u4f38\u8bd5\u9a8c\u4e0b\u7684\u6807\u79f0\u5e94\u529b\u4e0e\u5e94\u53d8\u7684\u4ee3\u8868\u56fe\u3002<\/p>\n<p>\uff08b\uff09\u5faa\u73af\u5265\u79bb\u8bd5\u9a8c\u4e0b\u6c34\u51dd\u80f6\u2014SiO<sub>2<\/sub>\u754c\u9762\u7684\u88c2\u7eb9\u6269\u5c55\u901f\u7387\u4e0e\u80fd\u91cf\u91ca\u653e\u901f\u7387\u7684\u5173\u7cfb\u3002<\/p>\n<p>\uff08c\uff09\u5faa\u73af\u62c9\u4f38\u8bd5\u9a8c\u4e0b\u6c34\u51dd\u80f6\u7ea4\u7ef4\u7684\u5e94\u53d8\u4e0e\u5faa\u73af\u6b21\u6570\u7684\u5173\u7cfb\u56fe\u3002<\/p>\n<p>\uff08d\uff09\u5faa\u73af\u62c9\u4f38\u8bd5\u9a8c\u4e2d\u4e0d\u540c\u62c9\u4f38\u5e94\u529b\u4e0b\u6c34\u51dd\u80f6\u7ea4\u7ef4\u7684\u663e\u5fae\u56fe\u50cf\u3002<\/p>\n<p>\uff08e-f\uff09\u6c34\u51dd\u80f6\u7ea4\u7ef4\u5728\u690d\u5165\u524d\u548c\u690d\u51658\u5468\u540e\uff0c\u5faa\u73af\u62c9\u4f38\u8bd5\u9a8c\u4e0b\u7684\u5e94\u529b\u4e0e\u5faa\u73af\u6b21\u6570\u7684\u5173\u7cfb\u3002<\/p>\n<p>\uff08g\uff09\u4e24\u7aef\u5206\u522b\u8fde\u63a5\u5149\u6e90\u548c\u5149\u7535\u63a2\u6d4b\u5668\u7684\u6c34\u51dd\u80f6\u5149\u7ea4\u5728\u4e0d\u540c\u62c9\u4f38\u5e94\u53d8\u4e0b\u7684\u56fe\u50cf\u3002<\/p>\n<p>\uff08h\uff09\u6c34\u51dd\u80f6\u7ea4\u7ef4\u7684\u5e94\u53d8\u548c\u5f52\u4e00\u5316\u5149\u5f3a\u5ea6\u4e0e\u5faa\u73af\u6b21\u6570\u7684\u4ee3\u8868\u56fe\u3002<\/p>\n<p>\uff08i\uff09\u4e8c\u6c27\u5316\u7845\u3001PDMS\u548c\u6c34\u51dd\u80f6\u7ea4\u7ef4\u5728PBS\u4e2d\u7684\u5f52\u4e00\u5316\u5149\u5f3a\u5ea6\u4e0e\u62c9\u4f38\u5e94\u53d8\u7684\u5173\u7cfb\u3002<\/p>\n<p>\uff08j\uff09\u6c34\u51dd\u80f6\u7ea4\u7ef4\u4e0e\u5404\u79cd\u900f\u660e\u6750\u6599\u7684\u8870\u51cf\u7cfb\u6570\u548c\u6768\u6c0f\u6a21\u91cf\u7684\u5bf9\u6bd4\u3002<\/p>\n<p>\uff08k\uff09\u65e0\u7ea4\u7ef4\u7684\u732a\u52a8\u8109\u3001\u9644\u7740\u4e8c\u6c27\u5316\u7845\u7ea4\u7ef4\u7684\u52a8\u8109\u548c\u9644\u7740\u6c34\u51dd\u80f6\u7ea4\u7ef4\u7684\u52a8\u8109\u7684\u56fe\u50cf\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-255641\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2023\/11\/3-3.png\" alt=\"\" width=\"600\" height=\"407\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2023\/11\/3-3.png 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2023\/11\/3-3-300x204.png 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p><strong>\u56fe<\/strong><strong>3\u00a0 <\/strong><strong>\u901a\u8fc7\u6c34\u51dd\u80f6\u5149\u7ea4\u53ef\u4ee5\u523a\u6fc0<\/strong><strong>ChR2<\/strong><strong>\u8001\u9f20\u7684\u5750\u9aa8\u795e\u7ecf<\/strong><strong> \u00a9 2023 Springer Nature<\/strong><\/p>\n<p>\uff08a\uff09\u5728Thy1:\uff1aChR2 EYFP\u8f6c\u57fa\u56e0\u5c0f\u9f20\u4e2d\u7528\u6c34\u51dd\u80f6\u7ea4\u7ef4\u5bf9\u540e\u80a2\u808c\u8089\u7684\u5149\u9057\u4f20\u5b66\u523a\u6fc0\u7684\u793a\u610f\u56fe\u3002<\/p>\n<p>\uff08b\uff09ChR2 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<\/strong><strong>\u901a\u8fc7\u6c34\u51dd\u80f6\u5149\u7ea4\u6291\u5236<\/strong><strong>NpHR<\/strong><strong>\u8001\u9f20\u7684\u6162\u6027\u75bc\u75db<\/strong><strong> \u00a9 2023 Springer Nature<\/strong><\/p>\n<p>\uff08a\uff09\u5229\u7528\u57fa\u56e0\u6742\u4ea4\u65b9\u6cd5\u4ea7\u751fTRPV1::NpHR-EYFP\u8f6c\u57fa\u56e0\u5c0f\u9f20\u7684\u793a\u610f\u56fe\u3002<\/p>\n<p>\uff08b\uff09pHR-EYFP\u8868\u8fbe\u7684DRG\u548c\u5750\u9aa8\u795e\u7ecf\u6a2a\u622a\u9762\u5171\u805a\u7126\u56fe\u50cf\u3002<\/p>\n<p>\uff08c\uff09\u4f53\u5185\u7814\u7a76\u7684\u8bbe\u8ba1\u548c8\u5468\u65f6\u95f4\u5b89\u6392\uff0c\u5305\u62ec\u690d\u5165\u3001\u6ce8\u5c04CFA\u548cIFA\u8bf1\u5bfc\u708e\u6027\u75bc\u75db\u3001\u75db\u89c9\u6d4b\u8bd5\u3001\u5149\u9057\u4f20\u5b66\u6291\u5236\u548c\u7ec4\u7ec7\u6536\u96c6\u3002<\/p>\n<p>\uff08d\uff09\u690d\u5165\u524d\uff08Baseline\uff09\u3001\u690d\u5165\u540e\u7b2c6\u5929CFA\u6ce8\u5c04\u524d\uff08Pre-CFA\u524d\uff09\u548c\u690d\u5165\u540e\u7b2c7\u5929CFA\u540e\uff08Post-CFA\uff09\u540c\u4fa7\u540e\u722a\u9000\u7f29\u7684\u4f24\u5bb3\u611f\u53d7\u9608\u503c\uff0c\u901a\u8fc7von Frey\u8bd5\u9a8c\u786e\u8ba4CFA\u8bf1\u5bfc\u7684\u708e\u75c7\u3002<\/p>\n<p>\uff08e\uff09\u6c34\u51dd\u80f6\u5149\u7ea4\u5c0f\u9f20\u70ed\u677f\u8bd5\u9a8c\u793a\u610f\u56fe\u3002<\/p>\n<p>\uff08f\uff09\u901a\u8fc7\u70ed\u677f\u8bd5\u9a8c\u8bc4\u4f30 TRPV1::NpHR-EYFP\u548cTRPV1::Cre\u5c0f\u9f20\u7b2c3\u5468\u81f3\u7b2c8\u5468\u7684\u70ed\u75db\u89c9\u654f\u611f\u6027\u65f6\uff0c\u540c\u4fa7\u548c\u5bf9\u4fa7\u540e\u722a\u5728\u6291\u5236\u8fc7\u7a0b\u4e2d\u9996\u6b21\u6536\u7f29\u7684\u5ef6\u8fdf\u65f6\u95f4\u3002<\/p>\n<p>\uff08g\uff09TRPV1::NpHR-EYFP\u5c0f\u9f20\u5728\u70ed\u677f\u8bd5\u9a8c\u4e2d\u9996\u6b21\u540c\u4fa7\u540e\u722a\u6536\u7f29\u7684\u6f5c\u4f0f\u671f\u968f\u690d\u5165\u540e\u65f6\u95f4\u7684\u53d8\u5316\u3002<\/p>\n<p>\uff08h\uff09\u4f7f\u7528\u6c34\u51dd\u80f6\u5149\u7ea4\u7684\u5c0f\u9f20\u5728von Frey\u8bd5\u9a8c\u4e2d\u7684\u793a\u610f\u56fe\u3002<\/p>\n<p>\uff08i\uff09\u7b2c3\u5468\u81f3\u7b2c8\u5468\uff0c\u901a\u8fc7von 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Frey\u8bd5\u9a8c\u4e2d\u540c\u4fa7\u540e\u722a\u6536\u7f29\u7684\u75db\u89c9\u9608\u503c\u3002<\/p>\n<p><strong>\u00a0<\/strong><strong>\u4e94\u3001\u3010\u6210\u679c\u542f\u793a\u3011<\/strong><\/p>\n<p>\u603b\u4e4b\uff0c\u6297\u75b2\u52b3\u6c34\u51dd\u80f6\u5c55\u793a\u4e86\u5728\u8eab\u4f53\u8fd0\u52a8\u8fc7\u7a0b\u4e2d\u5411\u5916\u5468\u795e\u7ecf\u4f20\u5bfc\u5149\u7684\u4e00\u79cd\u53ef\u80fd\u3002\u5728\u672a\u6765\uff0c\u4f5c\u8005\u8fd8\u5728\u63a2\u7d22\u5176\u4ed6\u7684\u6750\u6599\u8bbe\u8ba1\u548c\u529f\u80fd\u3002\u6bd4\u5982\uff0c\u5c06\u8fd9\u79cd\u6c34\u51dd\u80f6\u5149\u7ea4\u548c\u7535\u751f\u7406\u5b66\u7684\u67d4\u6027\u7535\u6781\u7ed3\u5408\uff0c\u5b9e\u73b0\u5149\u5b66\u63a7\u5236\u548c\u7535\u5b66\u6d4b\u91cf\u3002\u53e6\u5916\uff0c\u8fd9\u79cd\u5149\u7ea4\u4e0d\u4ec5\u53ef\u4ee5\u5c06\u5149\u4f20\u9001\u5230\u7279\u5b9a\u795e\u7ecf\u8fdb\u884c\u5149\u9057\u4f20\u5b66\u523a\u6fc0\uff0c\u8fd8\u53ef\u4ee5\u901a\u8fc7\u4f7f\u7528\u79bb\u5b50\u548c\u795e\u7ecf\u9012\u8d28\u7684\u8367\u5149\u6307\u793a\u5242\u4ece\u795e\u7ecf\u4e2d\u63d0\u53d6\u5149\u8fdb\u884c\u5149\u5b66\u8bb0\u5f55\u3002\u8fd9\u79cd\u6c34\u51dd\u80f6\u5149\u7ea4\u548c\u5149\u9057\u4f20\u5b66\u8054\u7528\u7684\u65b9\u6cd5\u4e5f\u53ef\u4ee5\u7528\u4e8e\u5916\u5468\u795e\u7ecf\u4ee5\u5916\u7684\u5176\u4ed6\u5668\u5b98\uff0c\u6bd4\u5982\u5fc3\u810f\u548c\u80a0\u9053\u3002\u67d4\u6027\u5149\u7ea4\u6f5c\u5728\u4e5f\u6709\u975e\u5e38\u5e7f\u6cdb\u7684\u4e34\u5e8a\u5e94\u7528\uff0c\u6bd4\u5982\u6cbb\u7597\u611f\u89c9\u969c\u788d\uff0c\u6162\u6027\u75bc\u75db\u548c\u4e2d\u98ce\u540e\u8fd0\u52a8\u6062\u590d\u7b49\u3002<\/p>\n<p><strong>\u539f\u6587\u8be6\u60c5<\/strong>\uff1a<a href=\"https:\/\/www.nature.com\/articles\/s41592-023-02020-9\" target=\"_blank\" rel=\"noopener\">Fatigue-resistant hydrogel optical fibers enable peripheral nerve optogenetics during locomotion<\/a> (<em>Nat. Methods <\/em><strong>2023<\/strong>, <em>120<\/em>, DOI: 10.1038\/s41592-023-02020-9)<\/p>\n<p>\u672c\u6587\u7531\u5927\u5175\u54e5\u4f9b\u7a3f\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u4e00\u3001\u3010\u5bfc\u8bfb\u3011 \u5149\u9057\u4f20\u5b66\u901a\u8fc7\u5bf9\u7279\u5b9a\u7ec6\u80de\u7fa4\u7684\u6d3b\u52a8\u8fdb\u884c\u7cbe\u786e\u63a7\u5236\uff0c\u6539\u53d8\u4e86\u7cfb\u7edf\u795e\u7ecf\u79d1\u5b66\u9886\u57df\u3002\u867d\u7136\u5149\u9057\u4f20\u5b66\u5df2\u88ab\u5e38\u89c4\u5e94\u7528\u4e8e\u5927\u8111\u56de\u8def\u7814\u7a76\uff0c\u4f46\u5c06\u5176\u5e94\u7528\u4e8e\u5267\u70c8\u8fd0\u52a8\u7684\u5916\u5468\u795e\u7ecf\u4ecd\u5177\u6709\u6311&#8230;<\/p>\n","protected":false},"author":1004,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[215],"tags":[],"class_list":["post-255637","post","type-post","status-publish","format-standard","hentry","category-jiedu"],"_links":{"self":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/255637"}],"collection":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/users\/1004"}],"replies":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=255637"}],"version-history":[{"count":1,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/255637\/revisions"}],"predecessor-version":[{"id":255643,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/255637\/revisions\/255643"}],"wp:attachment":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=255637"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=255637"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=255637"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}