{"id":248674,"date":"2023-03-26T06:11:02","date_gmt":"2023-03-25T22:11:02","guid":{"rendered":"\/\/m.iemloyee.com\/?p=248674"},"modified":"2023-03-22T15:13:02","modified_gmt":"2023-03-22T07:13:02","slug":"%e5%8c%97%e6%b8%85%e8%81%94%e6%89%8bnat-methods%ef%bc%9a%e8%b6%85%e5%b9%b3%e6%95%b4%e7%9f%b3%e5%a2%a8%e7%83%af%e5%88%b6%e5%a4%87%e5%9d%87%e5%8c%80%e7%9a%84%e8%96%84%e5%86%b0%e7%94%a8%e4%ba%8e%e9%ab%98","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=248674","title":{"rendered":"\u5317\u6e05\u8054\u624bNat Methods\uff1a\u8d85\u5e73\u6574\u77f3\u58a8\u70ef\u5236\u5907\u5747\u5300\u7684\u8584\u51b0\u7528\u4e8e\u9ad8\u5206\u8fa8\u7387\u51b7\u51bb\u7535\u955c\u7ed3\u6784\u5206\u6790"},"content":{"rendered":"<p>&nbsp;<\/p>\n<p style=\"text-align: center;\"><strong>\u4e00\u3001<\/strong> <strong>\u3010\u5bfc\u8bfb\u3011<\/strong><strong>\u00a0 <\/strong><\/p>\n<p>\u51b7\u51bb\u7535\u5b50\u663e\u5fae\u955c\uff08cryo-EM\uff09\u5df2\u7ecf\u6210\u4e3a\u7ed3\u6784\u751f\u7269\u5b66\u9886\u57df\u4e0d\u53ef\u6216\u7f3a\u7684\u5de5\u5177\u3002\u968f\u7740cryo-EM\u7ed3\u6784\u89e3\u6790\u5206\u8fa8\u7387\u7684\u4e0d\u65ad\u63d0\u9ad8\uff0c\u5236\u5907\u5747\u5300\u8584\u51b0\u6210\u4e3a\u5b9e\u73b0\u9ad8\u5206\u8fa8\u7387\u7ed3\u6784\u6d4b\u5b9a\u7684\u5173\u952e\u6b65\u9aa4\uff0c\u5c24\u5176\u662f\u5bf9\u4e8e\u5206\u5b50\u91cf\u5c0f\u4e8e100 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\u00c5\uff0c2.6 \u00c5\u548c2.2 \u00c5\u3002\u76f8\u5173\u6210\u679c\u4ee5\u201cUniform thin ice on ultraflat graphene for high-resolution cryo-EM\u201d\u53d1\u8868\u5728<em>Nature Methods<\/em>\u4e0a\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-248675\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2023\/03\/0-11.png\" alt=\"\" width=\"554\" height=\"248\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2023\/03\/0-11.png 554w, \/\/m.iemloyee.com\/wp-content\/uploads\/2023\/03\/0-11-300x134.png 300w\" sizes=\"(max-width: 554px) 100vw, 554px\" \/><\/p>\n<p style=\"text-align: center;\"><strong>\u00a0<\/strong><strong>\u4e09\u3001\u3010\u6838\u5fc3\u521b\u65b0\u70b9\u3011<\/strong><\/p>\n<p>\u5728\u672c\u6587\u4e2d\uff0c\u4f5c\u8005\u63d0\u51fa\u4e86\u4e00\u79cd\u65e0\u8936\u76b1\u7684\u9884\u62c9\u4f38\u8d85\u5e73\u6574\u77f3\u58a8\u70ef\uff08UFG\uff09\u4f5c\u4e3a\u5747\u5300\u8584\u51b0\u5236\u5907\u7684\u652f\u6491\u819c\u3002UFG\u7684\u5e73\u6574\u8868\u9762\u4f7f\u5f97\u6837\u54c1\u4e2d\u7684\u76ee\u6807\u9897\u7c92\u80fd\u591f\u5438\u9644\u5230\u5176\u8868\u9762\u4e0a\uff0c\u5904\u4e8e\u540c\u4e00\u5e73\u9762\uff0c\u4ece\u800c\u5c06\u5b83\u4eec\u4e0e\u7a7a\u6c14-\u6c34\u754c\u9762\u9694\u79bb\u5f00\u6765\uff0c\u5e76\u4e14\u4fdd\u8bc1\u4e86\u8584\u51b0\u7684\u5747\u5300\u6027\u3002<\/p>\n<p style=\"text-align: center;\"><strong>\u00a0<\/strong><strong>\u56db\u3001\u3010\u6570\u636e\u6982\u89c8\u3011<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-248676\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2023\/03\/1-11.png\" alt=\"\" width=\"1269\" height=\"628\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2023\/03\/1-11.png 1269w, \/\/m.iemloyee.com\/wp-content\/uploads\/2023\/03\/1-11-300x148.png 300w, \/\/m.iemloyee.com\/wp-content\/uploads\/2023\/03\/1-11-768x380.png 768w, \/\/m.iemloyee.com\/wp-content\/uploads\/2023\/03\/1-11-1024x507.png 1024w\" sizes=\"(max-width: 1269px) 100vw, 1269px\" \/><\/p>\n<p>\u56fe1\uff1a\u77f3\u58a8\u70ef\u7684\u7c97\u7cd9\u5ea6\u5f71\u54cd\u4e86\u51b0\u539a\u5ea6\u7684\u5747\u5300\u6027\u3002\u00a9 2022 The Authors<\/p>\n<p>a.\u793a\u610f\u56fe\u663e\u793a\uff0c\u6709\u76b1\u8936\u4e0d\u5e73\u6574\u7684\u77f3\u58a8\u70ef\u4f1a\u5bfc\u81f4\u6ce2\u6d6a\u5f62\u7684\u51b0\u5c42\u3002\u51b0\u5c42\u539a\u5ea6\u4e0d\u4e00\u81f4\uff0c\u8fd9\u53ef\u80fd\u5bfc\u81f4\u9897\u7c92\u5206\u5e03\u4ea7\u751f10-20\u7eb3\u7c73\u7684\u9ad8\u5ea6\u5dee\u5f02\uff08\u0394H\uff09\u3002\u5728\u5bf9\u503e\u8f6c\u6837\u54c1\u6210\u50cf\u65f6\uff0c\u86cb\u767d\u8d28\u9897\u7c92\u4fe1\u53f7\u88ab\u6709\u76b1\u8936\u7684\u77f3\u58a8\u70ef\u6240\u906e\u6321\u3002b. \u793a\u610f\u56fe\u663e\u793a\uff0c\u8d85\u5e73\u6574\u77f3\u58a8\u70ef\uff08UFG\uff09\u4f1a\u5f62\u6210\u5747\u5300\u7684\u51b0\u5c42\uff0c\u5e76\u4f7f\u86cb\u767d\u8d28\u9897\u7c92\u5438\u9644\u5728UFG\u8868\u9762\uff0c\u5206\u5e03\u5728\u540c\u4e00\u9ad8\u5ea6\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-248677\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2023\/03\/2-12.png\" alt=\"\" width=\"1269\" height=\"1132\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2023\/03\/2-12.png 1269w, \/\/m.iemloyee.com\/wp-content\/uploads\/2023\/03\/2-12-300x268.png 300w, \/\/m.iemloyee.com\/wp-content\/uploads\/2023\/03\/2-12-768x685.png 768w, \/\/m.iemloyee.com\/wp-content\/uploads\/2023\/03\/2-12-1024x913.png 1024w\" sizes=\"(max-width: 1269px) 100vw, 1269px\" \/><\/p>\n<p>\u56fe2\uff1a\u8d85\u5e73\u6574\u77f3\u58a8\u70ef\u819c\u7684\u8bbe\u8ba1\u548c\u6279\u91cf\u5236\u5907\u3002\u00a9 2022 The Authors<\/p>\n<p>a\u3001\u94dc\u7b94\u4e0a\u751f\u957f\u7684\u7c97\u7cd9\u77f3\u58a8\u70ef\u819c\u7684\u7167\u7247\u3002 b\u3001\u7c97\u7cd9\u77f3\u58a8\u70ef\u7684\u539f\u5b50\u529b\u663e\u5fae\u955c\uff08AFM\uff09\u56fe\u50cf\uff08\u4e0a\uff09\u548c\u76f8\u5e94\u7684\u793a\u610f\u56fe\uff08\u4e0b\uff09\uff0c\u663e\u793a\u94dc\u7b94\u8868\u9762\u7684\u7c97\u7cd9\u77f3\u58a8\u70ef\u5177\u6709\u5bc6\u96c6\u7684\u8936\u76b1\u3002 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\/\/m.iemloyee.com\/wp-content\/uploads\/2023\/03\/3-13-768x734.png 768w, \/\/m.iemloyee.com\/wp-content\/uploads\/2023\/03\/3-13-1024x978.png 1024w\" sizes=\"(max-width: 1269px) 100vw, 1269px\" \/><\/p>\n<p>\u56fe3\uff1a\u9884\u62c9\u4f38\u7684\u8d85\u5e73\u6574\u77f3\u58a8\u70ef\u4f7f\u51b0\u5c42\u539a\u5ea6\u66f4\u5747\u5300\u3002\u00a9 2022 The Authors<\/p>\n<p>a, \u7eb3\u7c73\u538b\u75d5\u5b9e\u9a8c\u7684\u793a\u610f\u56fe\u3002b\u3001c\uff0c\u8d85\u5e73\u6574\u77f3\u58a8\u70ef\u548c\u7c97\u7cd9\u77f3\u58a8\u70ef\u7684\u529b\u5b66\u5f3a\u5ea6(b)\u548c\u9884\u5f20\u529b(c)\u7684\u7edf\u8ba1\u76f4\u65b9\u56fe\u3002\u8d85\u5e73\u6574\u77f3\u58a8\u70ef\u5177\u67090.2N\/m\u9884\u5f20\u529b\u3002d\u3001e, 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loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-248679\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2023\/03\/4-10.png\" alt=\"\" width=\"1269\" height=\"1155\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2023\/03\/4-10.png 1269w, \/\/m.iemloyee.com\/wp-content\/uploads\/2023\/03\/4-10-300x273.png 300w, \/\/m.iemloyee.com\/wp-content\/uploads\/2023\/03\/4-10-768x699.png 768w, \/\/m.iemloyee.com\/wp-content\/uploads\/2023\/03\/4-10-1024x932.png 1024w\" sizes=\"(max-width: 1269px) 100vw, 1269px\" \/><\/p>\n<p>\u56fe4\uff1a\u8d85\u5e73\u6574\u77f3\u58a8\u70ef\u548c\u7c97\u7cd9\u77f3\u58a8\u70ef\u5236\u5907\u7684\u51b7\u51bb\u6837\u54c1\u7684\u7535\u955c\u6210\u50cf\u6548\u679c\u7684\u8868\u5f81\u3002\u00a0 \u00a9 2022 The 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\u7c97\u7cd9\u77f3\u58a8\u70ef\u652f\u6491\u7684\u51b7\u51bb\u6837\u54c1\u548c\u8d85\u5e73\u6574\u77f3\u58a8\u70ef\u652f\u6491\u7684\u51b7\u51bb\u6837\u54c1\u7684\u9897\u7c92\u6b20\u7126\u503c\u5206\u5e03\u4e0d\u540c\uff0c\u8bf4\u660e\u7c97\u7cd9\u77f3\u58a8\u70ef\u652f\u6491\u7684\u51b7\u51bb\u6837\u54c1\u4e2d\u9897\u7c92\u7684\u9ad8\u5ea6\u5206\u5e03\u5dee\u5f02\u66f4\u5927\u3002j. \u6837\u54c1\u9897\u7c92\u968f\u7740\u6210\u50cf\u5242\u91cf\u79ef\u7d2f\u800c\u4ea7\u751f\u6f02\u79fb\u73b0\u8c61\uff0c\u4f46\u9897\u7c92\u5728\u8d85\u5e73\u6574\u77f3\u58a8\u70ef\u652f\u6491\u7684\u51b7\u51bb\u6837\u54c1\u4e2d\u79fb\u52a8\u66f4\u5c0f\u3002k. Guinier\u56fe\u8868\u660e\uff0c\u8d85\u5e73\u6574\u77f3\u58a8\u70ef\u652f\u6491\u7684\u51b7\u51bb\u6837\u54c1\u7684\u56fe\u50cf\u8d28\u91cf\u66f4\u597d\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-248680\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2023\/03\/5-5.png\" alt=\"\" width=\"1269\" height=\"1267\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2023\/03\/5-5.png 1269w, \/\/m.iemloyee.com\/wp-content\/uploads\/2023\/03\/5-5-150x150.png 150w, \/\/m.iemloyee.com\/wp-content\/uploads\/2023\/03\/5-5-300x300.png 300w, \/\/m.iemloyee.com\/wp-content\/uploads\/2023\/03\/5-5-768x767.png 768w, \/\/m.iemloyee.com\/wp-content\/uploads\/2023\/03\/5-5-1024x1022.png 1024w\" sizes=\"(max-width: 1269px) 100vw, 1269px\" \/><\/p>\n<p>\u56fe5\uff1a\u5355\u9897\u7c92\u51b7\u51bb\u7535\u955c\u5bf9\u4e09\u4e2a\u5206\u5b50\u91cf\u8f83\u5c0f\u7684\u751f\u7269\u6837\u54c1\u8fdb\u884c\u9ad8\u5206\u8fa8\u7387\u7ed3\u6784\u6d4b\u5b9a\u3002\u00a9 2022 The Authors<\/p>\n<p>a\u3001d\u3001g\uff1a\u8d85\u5e73\u6574\u77f3\u58a8\u70ef\u652f\u6491\u7684\u8840\u7ea2\u86cb\u767d(a)\u3001\u03b1-\u80ce\u86cb\u767d(d)\u548c\u94fe\u9709\u4eb2\u548c\u7d20(g)\u7684\u51b7\u51bb\u7535\u955c\u56fe\u50cf\u3002\u63d2\u56fe\u663e\u793a\u4e86\u9897\u7c92\u7684\u4e8c\u7ef4\u5206\u7c7b\u5e73\u5747\u7ed3\u679c\u3002\u6bd4\u4f8b\u5c3a\u4e3a20\u2009nm\u3002b\u3001e\u3001h\uff1a\u6700\u7ec8\u91cd\u6784\u5206\u8fa8\u7387\u4e3a3.5 \u00c5\u7684\u8840\u7ea2\u86cb\u767d\u5bc6\u5ea6\u56fe (b)\uff0c2.6 \u00c5\u5206\u8fa8\u7387\u7684\u03b1-\u80ce\u86cb\u767d(e)\uff0c2.2 \u00c5\u5206\u8fa8\u7387\u7684\u94fe\u9709\u4eb2\u548c\u7d20(h)\u3002c\u3001f\u3001i\uff1a\u4ece\u8840\u7ea2\u86cb\u767d\u5bc6\u5ea6(c)\u548c\u03b1-\u80ce\u86cb\u767d\u5bc6\u5ea6(f)\u4e2d\u9009\u62e9\u51fa\u6765\u7684\u5c40\u90e8\u7684\u5bc6\u5ea6\u4e0e\u76f8\u5e94\u7684\u539f\u5b50\u6a21\u578b\u642d\u5efa\u7ed3\u679c\uff1b\u4ece2.2 \u00c5\u94fe\u9709\u4eb2\u548c\u7d20\u5bc6\u5ea6\u4e2d\u63d0\u53d6\u7684\u4ee3\u8868\u6027\u6c28\u57fa\u9178\u6b8b\u57fa\u7684\u5bc6\u5ea6\u4e0e\u76f8\u5e94\u7684\u539f\u5b50\u6a21\u578b(i)\u3002<\/p>\n<p style=\"text-align: center;\"><strong>\u4e94\u3001\u3010\u6210\u679c\u542f\u793a\u3011<\/strong><\/p>\n<p>\u603b\u8a00\u4e4b\uff0c\u672c\u6587\u4ecb\u7ecd\u4e86\u4f7f\u7528\u5728Cu\uff08111\uff09\/\u84dd\u5b9d\u77f3\u6676\u7247\u4e0a\u751f\u957f\u7684\u8d85\u5e73\u6574\u77f3\u58a8\u70ef\uff0c\u901a\u8fc7\u9762\u5bf9\u9762\u8f6c\u79fb\u65b9\u6cd5\u6279\u91cf\u5316\u5236\u5907\u7684\u7535\u955c\u8f7d\u7f51\u3002\u4e0e\u5e38\u7528\u7684\u94dc\u7b94\u751f\u957f\u7684\u77f3\u58a8\u70ef\u652f\u6491\u819c\u76f8\u6bd4\uff0c\u8d85\u5e73\u6574\u77f3\u58a8\u70ef\u5177\u6709\u4f18\u5f02\u7684\u673a\u68b0\u5f3a\u5ea6\uff0c\u80fd\u591f\u66f4\u597d\u5730\u63a7\u5236\u5747\u5300\u8584\u51b0\uff0c\u63d0\u9ad8\u51b7\u51bb\u7535\u955c\u56fe\u50cf\u8d28\u91cf\u3002\u5229\u7528\u8d85\u5e73\u6574\u77f3\u58a8\u70ef\u8f7d\u7f51\uff0c\u4f5c\u8005\u89e3\u6790\u51fa\u8840\u7ea2\u86cb\u767d\u3001\u03b1-\u80ce\u86cb\u767d\u548c\u94fe\u9709\u4eb2\u548c\u7d20\u7684\u8f83\u9ad8\u5206\u8fa8\u7387\u7684\u51b7\u51bb\u7535\u955c\u7ed3\u6784\u3002\u53e6\u5916\uff0c\u8d85\u5e73\u6574\u77f3\u58a8\u70ef\u5c06\u53ef\u4ee5\u5b9e\u73b0\u66f4\u53ef\u63a7\u7684\u751f\u7269\u6d3b\u6027\u529f\u80fd\u5316\u4fee\u9970\uff0c\u9ad8\u4eb2\u548c\u529b\u548c\u751f\u7269\u53cb\u597d\u6027\u5730\u8bc6\u522b\u76ee\u6807\u751f\u7269\u5206\u5b50\u3002\u8fd9\u9879\u5de5\u4f5c\u4e3a\u5176\u4ed6\u4e8c\u7ef4\u6750\u6599\u5728\u7ed3\u6784\u751f\u7269\u5b66\u4e2d\u7684\u5e94\u7528\u63d0\u4f9b\u4e86\u542f\u793a\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u539f\u6587\u8be6\u60c5<\/strong>\uff1aZheng, L., Liu, N., Gao, X. et al. Uniform thin ice on ultraflat graphene for high-resolution cryo-EM. Nat Methods 20, 123\u2013130 (2023).<\/p>\n<p>https:\/\/doi.org\/10.1038\/s41592-022-01693-y<\/p>\n","protected":false},"excerpt":{"rendered":"<p>&nbsp; \u4e00\u3001 \u3010\u5bfc\u8bfb\u3011\u00a0 \u51b7\u51bb\u7535\u5b50\u663e\u5fae\u955c\uff08cryo-EM\uff09\u5df2\u7ecf\u6210\u4e3a\u7ed3\u6784\u751f\u7269\u5b66\u9886\u57df\u4e0d\u53ef\u6216\u7f3a\u7684\u5de5\u5177\u3002\u968f\u7740cryo-EM\u7ed3\u6784\u89e3\u6790\u5206\u8fa8\u7387\u7684\u4e0d\u65ad\u63d0\u9ad8\uff0c\u5236\u5907\u5747\u5300\u8584\u51b0\u6210&#8230;<\/p>\n","protected":false},"author":48614,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[],"class_list":["post-248674","post","type-post","status-publish","format-standard","hentry","category-keji"],"_links":{"self":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/248674"}],"collection":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/users\/48614"}],"replies":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=248674"}],"version-history":[{"count":3,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/248674\/revisions"}],"predecessor-version":[{"id":249101,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/248674\/revisions\/249101"}],"wp:attachment":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=248674"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=248674"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=248674"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}