{"id":244174,"date":"2022-11-18T06:02:41","date_gmt":"2022-11-17T22:02:41","guid":{"rendered":"\/\/m.iemloyee.com\/?p=244174"},"modified":"2022-11-17T19:02:49","modified_gmt":"2022-11-17T11:02:49","slug":"%e6%b8%85%e5%8c%97%e8%81%94%e5%90%88nature-commun-%ef%bc%9a%e6%96%b0%e5%9e%8b%e5%8a%9f%e8%83%bd%e5%8c%96%e7%9f%b3%e5%a2%a8%e7%83%af%e6%94%b9%e5%96%84%e5%86%b7%e5%86%bb%e7%94%b5%e9%95%9c%e4%bc%98","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=244174","title":{"rendered":"\u6e05\u5317\u8054\u5408Nature Commun.\uff1a\u65b0\u578b\u529f\u80fd\u5316\u77f3\u58a8\u70ef\u6539\u5584\u51b7\u51bb\u7535\u955c\u4f18\u52bf\u53d6\u5411"},"content":{"rendered":"<p><strong>\u4e00\u3001\u3010\u5bfc\u8bfb\u3011<\/strong><\/p>\n<p>2017\u5e74\u8bfa\u8d1d\u5c14\u5316\u5b66\u5956\u6388\u4e88Jacques Dubochet, Joachim Frank \u548cRichard Henderson\uff0c\u4ee5\u8868\u5f70\u4ed6\u4eec\u5bf9\u201c\u51b7\u51bb\u7535\u955c\u6280\u672f\u201d\u9886\u57df\u7684\u8d21\u732e\uff0c\u8be5\u5956\u88ab\u620f\u79f0\u4e3a\u201c\u4e00\u4e2a\u53d1\u7ed9\u4e86\u7269\u7406\u5b66\u5bb6\u7684\u8bfa\u8d1d\u5c14\u5316\u5b66\u5956\uff0c\u5956\u52b1\u4ed6\u4eec\u5e2e\u52a9\u4e86\u751f\u7269\u5b66\u5bb6\u201d\u3002\u65e9\u572820\u4e16\u7eaa70\u5e74\u4ee3\u79d1\u5b66\u5bb6\u4eec\u5c31\u5229\u7528\u51b7\u51bb\u7535\u955c\uff08Cryo-EM\uff09\u7814\u7a76\u75c5\u6bd2\u5206\u5b50\u7684\u7ed3\u6784\uff0c\u9996\u6b21\u63d0\u51fa\u4e86\u51b7\u51bb\u7535\u955c\u6280\u672f\u7684\u539f\u7406\u3001\u65b9\u6cd5\u4ee5\u53ca\u6d41\u7a0b\u7684\u6982\u5ff5\u3002\u5355\u9897\u7c92Cryo-EM\u4e09\u7ef4\u91cd\u6784\u6280\u672f\u662f\u76ee\u524d\u7528\u4e8e\u89e3\u6790\u751f\u7269\u5927\u5206\u5b50\u9ad8\u5206\u8fa8\u7387\u7ed3\u6784\u7684\u4e3b\u6d41\u624b\u6bb5\u4e4b\u4e00\u3002\u7136\u800c\uff0c\u9ad8\u8d28\u91cf\u7684Cryo-EM\u6837\u54c1\u5236\u5907\u4ecd\u7136\u9762\u4e34\u5f88\u591a\u6311\u6218\uff0c\u5982\u6c14\u6db2\u754c\u9762\u3001\u4f18\u52bf\u53d6\u5411\u548c\u80cc\u666f\u566a\u97f3\u7b49\uff0c\u6781\u5927\u5730\u9650\u5236\u4e86\u7ed3\u6784\u89e3\u6790\u6548\u7387\u3002<\/p>\n<p><strong>\u4e8c\u3001\u3010\u6210\u679c\u63a0\u5f71\u3011<\/strong><\/p>\n<p>\u8fd1\u65e5\uff0c<strong>\u6e05\u534e\u5927\u5b66\u738b\u5b8f\u4f1f\u6559\u6388\u3001\u9976\u71cf\u6559\u6388\u3001\u5218\u6960\u535a\u58eb\u548c\u5317\u4eac\u5927\u5b66\u5f6d\u6d77\u7433\u6559\u6388<\/strong>\u7b49\u4eba\u5408\u4f5c\u5728\u77e5\u540d\u671f\u520a<strong><em>Nature Communications<\/em><\/strong>\u4e0a\u4ee5\u9898\u4e3a\u201c<strong>Functionalized graphene grids with various charges for single-particle cryo-EM<\/strong>\u201d\u62a5\u544a\u901a\u8fc7\u91cd\u6c2e\u76d0\u5206\u5b50\u5bf9CVD\u751f\u957f\u7684\u77f3\u58a8\u70ef\u819c\u8fdb\u884c\u529f\u80fd\u5316\u4fee\u9970\u5f00\u53d1\u4e86\u5177\u6709\u4e0d\u540c\u7535\u8377\u6027\u8d28\u57fa\u56e2\u7684\u529f\u80fd\u5316\u77f3\u58a8\u70ef\u652f\u6491\u819c\uff0c\u7528\u4e8eCryo-EM\u6837\u54c1\u5236\u5907\u3002\u4e0e\u805a\u5408\u7269\u8f6c\u79fb\u6cd5\u76f8\u6bd4\uff0c\u77f3\u58a8\u70ef\u652f\u6491\u819c\u4ee5\u77f3\u8721\u4f5c\u4e3a\u8f6c\u79fb\u4ecb\u8d28\u53ef\u4ee5\u6d01\u51c0\u5730\u8f6c\u79fb\u5230\u7535\u955c\u8f7d\u7f51\u4e0a\uff0c\u5176\u6c61\u67d3\u66f4\u5c11\u3002\u901a\u8fc7\u5e94\u7528\u4e8e\u4e09\u79cd\u4e0d\u540c\u7c7b\u578b\u7684\u751f\u7269\u5927\u5206\u5b50\uff0c\u8bc1\u660e\u4e86\u9ad8\u4ea7\u7387\u529f\u80fd\u5316\u77f3\u58a8\u70ef\u7535\u955c\u8f7d\u7f51\u53ef\u4f7f\u5927\u5206\u5b50\u8fdc\u79bb\u6c14\u6db2\u754c\u9762\uff08AWI\uff09\uff0c\u5e76\u5b9e\u73b0\u4e86\u53ef\u8c03\u8282\u7684\u7c92\u5b50\u53d6\u5411\u5206\u5e03\uff0c\u4ee5\u5b9e\u73b0\u66f4\u7a33\u5065\u7684\u5355\u9897\u7c92\u51b7\u51bb\u7535\u955c\u7ed3\u6784\u6d4b\u5b9a\u3002\u7814\u7a76\u8868\u660e\uff0c\u8fd9\u79cd\u529f\u80fd\u5316\u77f3\u58a8\u70ef\u652f\u6491\u819c\u63d0\u4f9b\u4e86\u4e00\u79cd\u8f83\u4e3a\u53cb\u597d\u7684\u4f5c\u7528\u754c\u9762\uff0c\u6709\u52a9\u4e8e\u4fdd\u62a4\u751f\u7269\u5927\u5206\u5b50\u7684\u4e09\u7ef4\u7ed3\u6784\u3002\u6e05\u534e\u5927\u5b66\u751f\u547d\u79d1\u5b66\u5b66\u9662\u535a\u58eb\u751f\u9646\u53f6\u3001\u535a\u58eb\u540e\u5218\u6960\uff0c\u836f\u5b66\u9662\u535a\u58eb\u751f\u5218\u6c38\u6ce2\u4ee5\u53ca\u5317\u4eac\u5927\u5b66\u5316\u5b66\u4e0e\u5206\u5b50\u5de5\u7a0b\u5b66\u9662\u535a\u58eb\u6bd5\u4e1a\u751f\u90d1\u9ece\u660e\u4e3a\u672c\u6587\u5171\u540c\u7b2c\u4e00\u4f5c\u8005\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-244175\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/11\/0-8.png\" alt=\"\" width=\"600\" height=\"345\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/11\/0-8.png 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2022\/11\/0-8-300x173.png 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p><strong>\u4e09\u3001\u3010\u6838\u5fc3\u521b\u65b0\u70b9\u3011<\/strong><\/p>\n<p>\u5f00\u53d1\u4e86\u5177\u6709\u4e0d\u540c\u7535\u8377\u57fa\u56e2\u7684\u529f\u80fd\u5316\u77f3\u58a8\u70ef\u652f\u6491\u819c\uff0c\u7528\u4e8eCryo-EM\u6837\u54c1\u5236\u5907\uff0c\u53ef\u4f7f\u751f\u7269\u5927\u5206\u5b50\u8fdc\u79bbAWI\uff0c\u5e76\u5b9e\u73b0\u4e86\u53ef\u8c03\u8282\u7684\u7c92\u5b50\u53d6\u5411\u5206\u5e03\uff0c\u4ee5\u5b9e\u73b0\u66f4\u7a33\u5065\u7684\u5355\u9897\u7c92\u51b7\u51bb\u7535\u955c\u7ed3\u6784\u6d4b\u5b9a\u3002<\/p>\n<p><strong>\u56db\u3001\u3010\u8bba\u6587\u63a0\u5f71\u3011<\/strong><\/p>\n<p><strong>\u00a0<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-244176\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/11\/1-16.png\" alt=\"\" width=\"600\" height=\"485\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/11\/1-16.png 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2022\/11\/1-16-300x243.png 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/strong><\/p>\n<p><strong>\u56fe<\/strong><strong>1<\/strong><strong>\u3001\u77f3\u8721\u8f85\u52a9\u77f3\u58a8\u70ef\u5728<\/strong><strong>EM<\/strong><strong>\u652f\u6491\u819c\u4e0a\u8f6c\u79fb<\/strong><strong> \u00a92022 Hongwei Wang<\/strong><\/p>\n<p>\uff08a\uff09\u77f3\u8721\u8f85\u52a9\u7684\u77f3\u58a8\u70ef\u8f6c\u79fb\u5230EM\u652f\u6491\u819c\u4e0a\u7684\u793a\u610f\u56fe\u3002<\/p>\n<p>\uff08b\uff09\u77f3\u8721\u8f6c\u79fb\u7684\u77f3\u58a8\u70ef\u652f\u6491\u819c\u7684\u9ad8\u500d\u7387TEM\u56fe\u50cf\u3002<\/p>\n<p>\uff08c\uff09\u9ad8\u500d\u7387\u7684AFM\u56fe\u50cf\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-244177\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/11\/2-15.png\" alt=\"\" width=\"600\" height=\"545\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/11\/2-15.png 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2022\/11\/2-15-300x273.png 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p><strong>\u56fe\u4e8c\u3001\u529f\u80fd\u5316\u77f3\u58a8\u70ef\u652f\u6491\u819c\u7684\u8868\u5f81<\/strong><strong> \u00a92022 Hongwei Wang<\/strong><\/p>\n<p>\uff08a\uff09\u7528\u4e8e\u51b7\u51bb\u7535\u955c\u5206\u6790\u7684\u529f\u80fd\u5316\u77f3\u58a8\u70ef\u7684\u793a\u610f\u56fe\u3002<\/p>\n<p>\uff08b\uff09\u60ac\u6d6e\u529f\u80fd\u5316\u77f3\u58a8\u70ef\u819c\u7684\u4ee3\u8868\u6027TEM\u663e\u5fae\u7167\u7247\u3002<\/p>\n<p>\uff08c\uff09\u77f3\u58a8\u70ef\uff08\u7ea2\u8272\uff09\u548c\u529f\u80fd\u5316\u77f3\u58a8\u70ef\uff08\u84dd\u8272\uff09\u7684\u62c9\u66fc\u5149\u8c31\u3002<\/p>\n<p>\uff08d\uff09\u77f3\u58a8\u70ef\uff08\u7ea2\u8272\uff09\u548c\u5b98\u80fd\u5316\u77f3\u58a8\u70ef\uff08\u84dd\u8272\uff09\u7684\u79ef\u5206\u5e03\u62c9\u683c\u53cd\u5c04\u76f8\u5bf9\u5f3a\u5ea6\u3002<\/p>\n<p>\uff08e\uff09NFG\u548cSFG\u7684X\u5c04\u7ebf\u5149\u7535\u5b50\u5149\u8c31\u3002<\/p>\n<p>\uff08f\uff09\u77f3\u58a8\u70ef\u3001NFG\u548cSFG\u7684\u6c34\u63a5\u89e6\u89d2\uff08WAC\uff09\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-244178\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/11\/3-15.png\" alt=\"\" width=\"600\" height=\"467\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/11\/3-15.png 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2022\/11\/3-15-300x234.png 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p><strong>\u56fe\u4e09\u3001\u4e0d\u540c\u7535\u6027\u4fee\u9970\u7684\u77f3\u58a8\u70ef\u7528\u4e8e<\/strong><strong>Cryo-EM<\/strong><strong>\u5206\u6790<\/strong><strong> \u00a92022 Hongwei Wang<\/strong><\/p>\n<p>\uff08a\uff09cryo-ET\u91cd\u5efa\u63ed\u793a\u4e8620S\u86cb\u767d\u9176\u4f53\u5728NFG\u819c\u4e0a\u7684\u5206\u5e03\u3002<\/p>\n<p>\uff08b\uff09\u4eceNFG\u652f\u6491\u7684\u6837\u54c1\u7684\u4f4e\u6e29\u5c42\u6790\u56fe\u4e2d\u63d0\u53d6\u7684\u77f3\u58a8\u70ef\u8868\u9762\u5256\u9762\u3002<\/p>\n<p>\uff08c\uff0920S\u86cb\u767d\u9176\u4f53\u5728NFG\u4e0a\u7684\u6b27\u62c9\u89d2\u5206\u5e03\u3002<\/p>\n<p>\uff08d\uff09cryo-ET\u91cd\u5efa\u663e\u793a20S\u86cb\u767d\u9176\u4f53\u5728SFG\u819c\u4e0a\u7684\u5206\u5e03\u3002<\/p>\n<p>\uff08e\uff09\u4eceSFG\u652f\u6491\u7684\u6837\u54c1\u7684\u4f4e\u6e29\u5c42\u6790\u56fe\u4e2d\u63d0\u53d6\u7684\u77f3\u58a8\u70ef\u8868\u9762\u5256\u9762\u3002<\/p>\n<p>\uff08f\uff0920S\u86cb\u767d\u9176\u4f53\u5728SFG\u4e0a\u7684\u6b27\u62c9\u89d2\u5206\u5e03\u3002<\/p>\n<p>\uff08g\uff0950S\u6838\u7cd6\u4f53\u5728NFG\u4e0a\u7684\u6b27\u62c9\u89d2\u5206\u5e03\u3002<\/p>\n<p>\uff08h\uff0950S\u6838\u7cd6\u4f53\u5728NFG\u4e0a\u7684\u9996\u9009\u89c6\u56fe\u3002<\/p>\n<p>\uff08i\uff0950S\u6838\u7cd6\u4f53\u5728SFG\u4e0a\u7684\u6b27\u62c9\u89d2\u5206\u5e03\u3002<\/p>\n<p>\uff08j\uff0950S\u6838\u7cd6\u4f53\u5728SFG\u4e0a\u7684\u9996\u9009\u89c6\u56fe\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-244179\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/11\/4-15.png\" alt=\"\" width=\"600\" height=\"410\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/11\/4-15.png 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2022\/11\/4-15-300x205.png 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p><strong>\u56fe\u56db\u3001\u5177\u6709\u4e0d\u540c\u7535\u8377\u7684\u77f3\u58a8\u70ef\u89e3\u51b3<\/strong><strong>L1 LtrB RNP<\/strong><strong>\u7684\u4f18\u5148\u53d6\u5411\u95ee\u9898<\/strong><strong>\u00a92022 Hongwei Wang<\/strong><\/p>\n<p>\uff08a-d\uff09L1.LtrB RNP\u590d\u5408\u7269\u5728NFG\u548cSFG\u4e0a\u7684\u9897\u7c92\u53d6\u5411\u5206\u5e03\u3002<\/p>\n<p>\uff08e\uff09\u4f7f\u7528NFG\u548cSFG\u7684\u7ec4\u5408\u9897\u7c92\u91cd\u5efa\u7684L1.LtrB RNP\u590d\u5408\u7269\u7684\u4e09\u7ef4\u91cd\u6784\u5bc6\u5ea6\u56fe\u3002<\/p>\n<p>\uff08f\uff09L1.LtrB RNP\u7684DV\u5bc6\u5ea6\u4e0e\u76f8\u5e94\u7684\u6a21\u578b\u5bf9\u63a5\uff08PDB: 8H2H\uff09\u3002<\/p>\n<p><strong>\u4e94\u3001\u3010\u524d\u666f\u5c55\u671b\u3011<\/strong><\/p>\n<p>\u7efc\u4e0a\u6240\u8ff0\uff0c\u7814\u7a76\u4eba\u5458\u5f00\u53d1\u4e86\u4e00\u79cd\u6709\u6548\u7684\u6e05\u6d01\u8f6c\u79fb\u7b56\u7565\uff0c\u4f7f\u7528\u77f3\u8721\u4f5c\u4e3a\u8f6c\u79fb\u5a92\u4ecb\u6765\u5236\u9020\u5e26\u7535\u7684\u77f3\u58a8\u70ef\u819c\uff0c\u7136\u540e\u901a\u8fc7\u91cd\u6c2e\u76d0\u5206\u5b50\u5bf9CVD\u751f\u957f\u7684\u77f3\u58a8\u70ef\u819c\u8fdb\u884c\u529f\u80fd\u5316\u4fee\u9970\u3002\u5e26\u7535\u7684\u77f3\u58a8\u70ef\u652f\u6491\u819c\u6839\u636e\u76ee\u6807\u5206\u5b50\u7684\u8868\u9762\u7535\u8377\u7279\u6027\uff0c\u4e3a\u86cb\u767d\u8d28\u5206\u5b50\u6216\u5927\u5206\u5b50\u590d\u5408\u7269\u63d0\u4f9b\u4e86\u4e0d\u540c\u6027\u8d28\u7684\u8868\u9762\uff0c\u4ee5\u4fbf\u4e0e\u4e4b\u76f8\u4e92\u4f5c\u7528\u3002\u56e0\u6b64\u80fd\u591f\u8c03\u63a7\u53d6\u5411\u5206\u5e03\uff0c\u907f\u514d\u4e86AWI\u5e26\u6765\u7684\u8d1f\u9762\u5f71\u54cd\uff0c\u6210\u529f\u89e3\u51b3\u4e86\u4f18\u52bf\u53d6\u5411\u95ee\u9898\u3002<\/p>\n<p>\u6587\u732e\u94fe\u63a5\uff1a<a href=\"https:\/\/www.nature.com\/articles\/s41467-022-34579-w\" target=\"_blank\" rel=\"noopener\">Functionalized graphene grids with various charges for single-particle cryo-EM<\/a> (<em>Nat. Commun., <\/em><strong>2022<\/strong>, <em>13<\/em>, 6718)<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u4e00\u3001\u3010\u5bfc\u8bfb\u3011 2017\u5e74\u8bfa\u8d1d\u5c14\u5316\u5b66\u5956\u6388\u4e88Jacques Dubochet, Joachim Frank \u548cRichard Henderson\uff0c\u4ee5\u8868\u5f70\u4ed6\u4eec\u5bf9\u201c\u51b7\u51bb&#8230;<\/p>\n","protected":false},"author":1004,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[215],"tags":[4962],"class_list":["post-244174","post","type-post","status-publish","format-standard","hentry","category-jiedu","tag-4962"],"_links":{"self":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/244174"}],"collection":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/users\/1004"}],"replies":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=244174"}],"version-history":[{"count":1,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/244174\/revisions"}],"predecessor-version":[{"id":244180,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/244174\/revisions\/244180"}],"wp:attachment":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=244174"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=244174"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=244174"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}