{"id":240728,"date":"2022-08-06T06:52:51","date_gmt":"2022-08-05T22:52:51","guid":{"rendered":"\/\/m.iemloyee.com\/?p=240728"},"modified":"2022-08-05T16:53:59","modified_gmt":"2022-08-05T08:53:59","slug":"%e5%93%a5%e5%a4%a7nature-nanotech%ef%bc%9a%e5%8f%af%e7%94%a8%e4%ba%8ecovid-19%e5%bf%ab%e9%80%9f%e6%a3%80%e6%b5%8b%e7%9a%84%e6%96%b0%e5%9e%8b%e5%a4%9a%e9%87%8d%e9%80%86%e8%bd%ac%e5%bd%95%e9%85%b6","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=240728","title":{"rendered":"\u54e5\u5927Nature Nanotech\uff1a\u53ef\u7528\u4e8eCOVID-19\u5feb\u901f\u68c0\u6d4b\u7684\u65b0\u578b\u591a\u91cd\u9006\u8f6c\u5f55\u9176\u5b9a\u91cfPCR\u6280\u672f"},"content":{"rendered":"<p>\u3010\u5bfc\u8bfb\u3011<\/p>\n<p>\u5e8a\u65c1\u68c0\u6d4b\uff08POC\uff09\u4e5f\u79f0\u73b0\u573a\u68c0\u6d4b\uff0c\u6216\u8fd1\u60a3\u8005\u68c0\u6d4b\uff0c\u5b83\u662f\u4e00\u79cd\u53ef\u5728\u77ed\u65f6\u95f4\u5185\u5c06\u60a3\u8005\u63d0\u4f9b\u7684\u6837\u672c\u8fdb\u884c\u68c0\u6d4b\u7684\u65b0\u5174\u6280\u672f\uff0c\u56e0\u5176\u5177\u6709\u65e0\u9700\u6837\u54c1\u5904\u7406\uff0c\u68c0\u6d4b\u65f6\u95f4\u77ed\uff0c\u6837\u672c\u9700\u6c42\u91cf\u5c11\uff0c\u53ef\u5728\u60a3\u8005\u8fd1\u65c1\u8fdb\u884c\u5b9e\u65f6\u76d1\u6d4b\u7684\u4f18\u70b9\uff0c\u800c\u88ab\u5e7f\u6cdb\u5173\u6ce8\u3002\u7ecf\u8fc7\u6570\u5341\u5e74\u7684\u53d1\u5c55\uff0c\u5e8a\u65c1\u68c0\u6d4b\u6280\u672f\u5df2\u5e94\u7528\u4e8e\u624b\u672f\u5ba4\uff0c\u91cd\u75c7\u76d1\u62a4\u5ba4\u6216\u6025\u75c7\u5ba4\u7b49\u573a\u666f\uff0c\u5bf9\u60a3\u8005\u7684\u751f\u5316\u6307\u6807\u8fdb\u884c\u68c0\u6d4b\u3002<\/p>\n<p>\u968f\u7740\u6750\u6599\u548c\u6280\u672f\u7684\u4e0d\u65ad\u53d1\u5c55\uff0c\u7814\u7a76\u4eba\u5458\u5e0c\u671b\u80fd\u5c06\u5e8a\u65c1\u68c0\u6d4b\u6280\u672f\u5e94\u7528\u5230\u805a\u5408\u9176\u94fe\u5f0f\u53cd\u5e94\uff08PCR\uff09\u6280\u672f\u4e2d\uff0c\u4ee5\u5b9e\u73b0\u4fbf\u643a\u5730\uff0c\u5feb\u901f\u5730\u6838\u9178\u68c0\u6d4b\uff0c\u7279\u522b\u662f\u8fd1\u4e24\u5e74COVID-19\u5728\u4e16\u754c\u8303\u56f4\u5185\u7684\u6d41\u884c\uff0c\u66f4\u9700\u8981\u4e00\u79cd\u5feb\u901f\u7684\u68c0\u6d4b\u624b\u6bb5\u3002\u7136\u800c\uff0c\u4f20\u7edf\u7684PCR\u6d4b\u8bd5\u73af\u5883\u8981\u6c42\u9ad8\uff0c\u6d4b\u8bd5\u8bbe\u5907\u5e9e\u5927\uff0c\u7279\u522b\u662f\u70ed\u5faa\u73af\u4eea\u7b49\uff0c\u9700\u8981\u5145\u8db3\u7684\u65f6\u95f4\u8fdb\u884c\u5347\u6e29\u548c\u964d\u6e29\uff0c\u56e0\u6b64\uff0c\u6025\u9700\u5f00\u53d1\u4e00\u79cd\u73b0\u573a\u68c0\u6d4b\u6838\u9178\u5e76\u5feb\u901f\u5f97\u5230\u7ed3\u679c\u7684\u6280\u672f\u3002<\/p>\n<p>\u3010\u6210\u679c\u63a0\u5f71\u3011<\/p>\n<p>\u8fd1\u65e5\uff0c\u6765\u81ea\u54e5\u4f26\u6bd4\u4e9a\u5927\u5b66\u7684Samuel K. Sia\u6559\u6388\u548c\u5176\u56e2\u961f\u6210\u5458\u5728\u81ea\u7136\u00b7\u7eb3\u7c73\u6280\u672f\uff08<em>Nature Nanotechnology<\/em>\uff09\u671f\u520a\u4e0a\u4ee5\u201cMultiplexed reverse-transcriptase quantitative polymerase chain reaction using plasmonic nanoparticles for point-of-care COVID-19 diagnosis\u201d\u4e3a\u9898\u62a5\u9053\u4e86\u4e00\u79cd\u53ef\u4ece\u4eba\u7c7b\u553e\u6db2\u6216\u9f3b\u8154\u6837\u672c\u4e2d\u5feb\u901f\u68c0\u6d4bSARS-CoV-2\u7684RNA\u7684\u5c0f\u578b\u5149\u5b66\u5668\u4ef6\u3002\u57fa\u4e8e\u8367\u5149\u63a2\u9488\u7684\u6d4b\u8bd5\u65b9\u6cd5\u548c\u7b49\u79bb\u5b50\u4f53\u7eb3\u7c73\u9897\u7c92\u7684\u589e\u5f3a\u4f5c\u7528\uff0c\u8be5\u5668\u4ef6\u53ef\u5728\u5355\u4e2a\u53cd\u5e94\u5bb9\u5668\u4e2d\u5b9e\u73b0\u9006\u8f6c\u5f55\u9176\u5b9a\u91cfPCR\uff08RT-qPCR\uff09\uff0c\u5e76\u5177\u6709\u7b49\u79bb\u5b50\u4f53\u70ed\u5faa\u73af\u671f\u95f4\u591a\u91cd\u8367\u5149\u76d1\u6d4b\u7684\u80fd\u529b\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-240729\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/08\/0-3.png\" alt=\"\" width=\"858\" height=\"514\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/08\/0-3.png 858w, \/\/m.iemloyee.com\/wp-content\/uploads\/2022\/08\/0-3-300x180.png 300w, \/\/m.iemloyee.com\/wp-content\/uploads\/2022\/08\/0-3-768x460.png 768w\" sizes=\"(max-width: 858px) 100vw, 858px\" \/><\/p>\n<p>\u3010\u6838\u5fc3\u521b\u65b0\u70b9\u3011<\/p>\n<ol>\n<li>\u8be5\u5de5\u4f5c\u9996\u521b\u6027\u7684\u5c06\u7528\u4e8e\u6cbb\u7597\u7684\u7b49\u79bb\u5b50\u4f53\u52a0\u70ed\u6280\u672f\u670d\u52a1\u4e8ePCR\u7684\u52a0\u70ed\u73af\u8282\uff0c\u89e3\u51b3\u4e86\u4f20\u7edfPCR\u6280\u672f\u6d4b\u8bd5\u65f6\u95f4\u957f\uff0c\u4eea\u5668\u5927\u7684\u96be\u9898\u3002<\/li>\n<li>\u8be5\u5de5\u4f5c\u91c7\u7528\u8367\u5149\u68c0\u6d4b\u8bc6\u522b\u7279\u5b9a\u6838\u9178\u7684\u65b9\u6cd5\uff0c\u53d6\u6d88\u4e86\u4ece\u6837\u672c\u5bb9\u5668\u4e2d\u53bb\u9664\u91d1\u7eb3\u7c73\u9897\u7c92\u7684\u73af\u8282\uff0c\u7f29\u77ed\u4e86\u6d4b\u8bd5\u65f6\u95f4\uff0c\u63d0\u9ad8\u4e86\u68c0\u6d4b\u6280\u672f\u7684\u751f\u7269\u5b89\u5168\u6027\u3002<\/li>\n<\/ol>\n<p>\u3010\u6570\u636e\u6982\u89c8\u3011<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-240730\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/08\/1-4.png\" alt=\"\" width=\"1029\" height=\"722\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/08\/1-4.png 1029w, \/\/m.iemloyee.com\/wp-content\/uploads\/2022\/08\/1-4-300x210.png 300w, \/\/m.iemloyee.com\/wp-content\/uploads\/2022\/08\/1-4-768x539.png 768w, \/\/m.iemloyee.com\/wp-content\/uploads\/2022\/08\/1-4-1024x718.png 1024w\" sizes=\"(max-width: 1029px) 100vw, 1029px\" \/><\/p>\n<p>\u56fe1. \u591a\u91cd\u5b9e\u65f6\u7b49\u79bb\u5b50\u4f53RT-PCR\u7684\u6d4b\u8bd5\u539f\u7406\u548c\u8bbe\u8ba1\u3002a\uff09\u591a\u91cd\u5b9e\u65f6\u7b49\u79bb\u5b50\u4f53RT-PCR\u7684\u793a\u610f\u56fe\uff0c\u91d1\u7eb3\u7c73\u9897\u7c92\u60ac\u6d6e\u57280.2ml PCR\u7ba1\u4e2d\u7684\u6eb6\u6db2\u4e2d\uff0c\u5176\u5feb\u901f\u5438\u6536LED\u7684\u5149\u5e76\u5c06\u5176\u8f6c\u5316\u4e3a\u70ed\u91cf\uff0c\u4ece\u800c\u5b9e\u73b0\u5feb\u901fPCR\u70ed\u5faa\u73af\uff1bb\uff09\u4eea\u5668\u793a\u610f\u56fe\uff1bc\uff09\u8367\u5149\u68c0\u6d4b\u7cfb\u7edf\u793a\u610f\u56fe\uff1bd\uff09\u7cfb\u7edf\u5185\u5404\u7ec4\u5206\u975e\u91cd\u53e0\u5149\u5b66\u5149\u8c31\u3002@2022 Springer Nature Limited<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-240731\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/08\/2-5.png\" alt=\"\" width=\"1028\" height=\"700\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/08\/2-5.png 1028w, \/\/m.iemloyee.com\/wp-content\/uploads\/2022\/08\/2-5-300x204.png 300w, \/\/m.iemloyee.com\/wp-content\/uploads\/2022\/08\/2-5-768x523.png 768w, \/\/m.iemloyee.com\/wp-content\/uploads\/2022\/08\/2-5-1024x697.png 1024w\" sizes=\"(max-width: 1028px) 100vw, 1028px\" \/><\/p>\n<p>\u56fe2. \u5feb\u901f\u3001\u591a\u91cd\u3001\u7b49\u79bb\u5b50\u4f53RT-PCR\u6d4b\u8bd5\u7ed3\u679c\u3002a\uff09\u63a7\u5236\u826f\u597d\u7684\u6e29\u5ea6\u5e8f\u5217\uff0c\u572815\u5206\u949f\u5185\u663e\u793a\u5b8c\u6574\u7684RT-PCR\uff1bb\uff09\u5bf9a\u6240\u793a\u7684\u6570\u636e\u8fdb\u4e00\u6b65\u5206\u6790\uff0c\u6574\u4e2a45\u4e2a\u5468\u671f\u5185\u5b9e\u73b0\u4e00\u81f4\u7684\u52a0\u70ed\u548c\u51b7\u5374\u901f\u7387\uff0c\u5e73\u5747\u52a0\u70ed\u901f\u7387\u4e3a6.7\u00b10.2\u00b0C\/s\uff0c\u5e73\u5747\u51b7\u5374\u901f\u7387\u4e3a \u20134.7 \u00b1 0.1 \u00b0C\/s\uff1bc\uff09\u7b49\u79bb\u5b50\u4f53RT-PCR\u7cfb\u7edf\u7684\u7ec8\u70b9\u8367\u5149\u6d4b\u91cf\u7684\u521d\u59cb\u9633\u6027\u6269\u589e\u7ed3\u679c\uff1bd\uff09\u4f7f\u7528\u7eaf\u5316\u7684RNA\u548c\u5feb\u901f\u6269\u589e\u7684\u521d\u59cb\u5149\u70ed\u6269\u589eLoD\u6570\u636e\uff08&lt;16\u5206\u949f\uff09\u3002@2022 Springer Nature Limited<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-240732\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/08\/3-5.png\" alt=\"\" width=\"675\" height=\"952\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/08\/3-5.png 675w, \/\/m.iemloyee.com\/wp-content\/uploads\/2022\/08\/3-5-213x300.png 213w\" sizes=\"(max-width: 675px) 100vw, 675px\" \/><\/p>\n<p>\u56fe3. \u96c6\u6210\u5b9e\u65f6\u8367\u5149\u68c0\u6d4b\uff0c\u65e0\u9700\u53bb\u9664AuNR\u3002a\uff09\u5728RT-PCR\u53cd\u5e94\u4e2d\u6dfb\u52a0AuNR\uff08OD\u4e3a18\uff09\u53ef\u6dec\u706d\u539f\u59cb\u8367\u5149\u4fe1\u53f7\u5bf9\u6bd4\u56fe\uff1bb\uff09\u52a0\u5165AuNR\u524d\u540e\u7684Ct\u503c\u5bf9\u6bd4\uff1bc\uff09\u4f7f\u7528\u5355\u4e2a\u6fc0\u53d1\u6fc0\u5149\u5668\u548c\u5149\u8c31\u4eea\u5b9e\u73b0\u591a\u5149\u8c31\u68c0\u6d4b\uff0c\u591a\u5143\u7ebf\u6027\u56de\u5f52\u5b9e\u73b0\u5149\u8c31\u53cd\u5377\u79ef\uff1bd\uff09\u5b9e\u65f6\u653e\u5927\u548c\u68c0\u6d4b\uff1be\uff09\u53cd\u5377\u79ef\u8367\u5149\u503c\uff08\u9488\u5bf9\u4e09\u4e2a\u5355\u72ec\u7684\u9776\u6807\/\u989c\u8272\uff09\u4e0e\u5468\u671f\u6570\u7684\u5bf9\u6bd4\uff0c\u4ee5\u8ba1\u7b97Ct\u503c\uff1bf\uff09AuNR OD\u5bf9\u52a0\u70ed\u901f\u7387\u7684\u5f71\u54cd\uff1bg\uff09AuNR OD\u5bf9\u8367\u5149\u76d1\u6d4b\u7684\u5f71\u54cd\u3002@2022 Springer Nature Limited<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-240733\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/08\/4-4.png\" alt=\"\" width=\"577\" height=\"976\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/08\/4-4.png 577w, \/\/m.iemloyee.com\/wp-content\/uploads\/2022\/08\/4-4-177x300.png 177w\" sizes=\"(max-width: 577px) 100vw, 577px\" \/><\/p>\n<p>\u56fe4. \u4f7f\u7528\u591a\u91cd\u7b49\u79bb\u5b50\u4f53RT-qPCR\u68c0\u6d4bSARS-CoV-2\u7684\u706d\u6d3b\u75c5\u6bd2\u548c\u4e34\u5e8a\u6807\u672c\u3002a\uff09\u7ec8\u70b9\u8367\u5149\u5149\u8c31\u68c0\u6d4b\uff1bb\uff09\u7b49\u79bb\u5b50\u4f53RT-qPCR\u8fd0\u884c\u7684\u5b9e\u65f6\u6269\u589e\u66f2\u7ebf\uff1bc\uff09N1\u548cRP\u7684Ct\u503c\uff1bd\uff09N1\uff0cN2\u548cRP\u9776\u6807\u7684\u989d\u5916\u6d53\u5ea6\u7684C<sub>t<\/sub>\u503c\uff1be\uff09N1 Ct\u503c\u4e0eQuantStudio\uff08QS\uff09\u6bd4\u8f83\uff1bf\uff09\u4e34\u5e8a\u553e\u6db2\u6807\u672c\u68c0\u6d4b\u5341\u4e2a\u9633\u6027\u548c\u4e5d\u4e2a\u9634\u6027\u6807\u672c\uff0c\u663e\u793a100%\u654f\u611f\u6027\u548c100%\u7279\u5f02\u6027\uff1bg\uff09\u63a5\u6536\u5668\u5de5\u4f5c\u7279\u6027\uff08ROC\uff09\u66f2\u7ebf\uff1bh\uff09\u4eea\u5668\u4e0eQuantStudio\u7684N1 C<sub>t<\/sub>\u503c\u7684\u6bd4\u8f83\uff1bi\uff09\u5bf9\u9776\u6807N1\u768420\u4e2a\u9633\u6027\u548c29\u4e2a\u9634\u6027\u6837\u672c\u8fdb\u884c\u4e34\u5e8a\u9f3b\u8154\u6807\u672c\u68c0\u6d4b\uff0c\u663e\u793a100%\u7684\u654f\u611f\u6027\u548c100%\u7684\u7279\u5f02\u6027\uff1bj\uff09\u5728i\u4e2d\u6d4b\u8bd5\u7684\u8bd5\u6837\u7684ROC\u66f2\u7ebf\uff1bk\uff09\u7528\u7b49\u79bb\u5b50\u4f53\u6d4b\u8bd5\u7684\u9f3b\u8154\u4e34\u5e8a\u6807\u672c\u4e0eQuantStudio\u6d4b\u8bd5\u7684N1 C<sub>t<\/sub>\u503c\u7684\u6bd4\u8f83\uff1bl\uff09\u4e34\u5e8a\u9f3b\u8154\u6807\u672c\u68c0\u6d4b20\u4f8b\u9633\u6027\u548c29\u4f8b\u9634\u6027\u6837\u672c\u7684\u9776N\u57fa\u56e0\uff0c\u654f\u611f\u6027\u4e3a100%\uff0c\u7279\u5f02\u6027\u4e3a100%\uff1bm\uff09\u5728l\u4e2d\u6d4b\u8bd5\u7684\u8bd5\u6837\u7684ROC\u66f2\u7ebf\uff1bn\uff09\u7528\u7b49\u79bb\u5b50\u4f53\u4e0eQuantStudio\u6d4b\u8bd5\u7684\u9f3b\u8154\u4e34\u5e8a\u6807\u672c\u7684N\u57fa\u56e0Ct\u503c\u7684\u6bd4\u8f83\uff1bo\uff09\u7ed8\u5236\u4e34\u5e8a\u6807\u672c\u8fde\u7eed\u7a00\u91ca\u7684N1 C<sub>t<\/sub>\u503c\u7684\u6807\u51c6\u66f2\u7ebf\uff1bp\uff09\u5728\u7b49\u79bb\u5b50\u4f53\u4eea\u5668\u4e0a\u6d4b\u8bd5B.1.1.7\u53d8\u4f53\u7684\u8fde\u7eed\u7a00\u91ca\u6837\u672c\uff1bq\uff09\u5bf9\u4e8e\u9488\u5bf9\u4e2d\u4e1c\u547c\u5438\u7efc\u5408\u5f81\uff08n = 23\uff09\u548c\u4eba\u7c7b\u51a0\u72b6\u75c5\u6bd2NL63\uff08n = 27\uff09\u8fdb\u884c\u6d4b\u8bd5\u3002@2022 Springer Nature Limited<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-240734\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/08\/5-2.png\" alt=\"\" width=\"849\" height=\"889\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/08\/5-2.png 849w, \/\/m.iemloyee.com\/wp-content\/uploads\/2022\/08\/5-2-287x300.png 287w, \/\/m.iemloyee.com\/wp-content\/uploads\/2022\/08\/5-2-768x804.png 768w\" sizes=\"(max-width: 849px) 100vw, 849px\" \/><\/p>\n<p>\u56fe5. 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Limited<\/p>\n<p>\u3010\u6210\u679c\u5c55\u793a\u3011<\/p>\n<p>\u8be5\u5de5\u4f5c\u5f00\u53d1\u4e86\u4e00\u79cd\u7b49\u79bb\u5b50\u4f53RT-qPCR\u6280\u672f\uff0c\u5728\u8bca\u65adCOVID-19\u548c\u5176\u4ed6\u4f20\u67d3\u75c5\u65b9\u9762\u5177\u6709\u4f17\u591a\u4f18\u70b9\uff1a1\uff09\u8be5\u6280\u672f\u65e0\u9700\u53bb\u9664\u6837\u672c\u5bb9\u5668\u5185\u7684\u91d1\u7eb3\u7c73\u9897\u7c92\uff0c\u786e\u4fdd\u4e86\u5176\u7528\u4e8e\u5e8a\u65c1\u68c0\u6d4b\u7684\u751f\u7269\u5b89\u5168\u6027\uff0c2\uff09\u8be5\u68c0\u6d4b\u6280\u672f\u5177\u6709\u8f83\u5f3a\u7684\u7279\u5f02\u6027\u53cd\u5e94\uff0c\u53ef\u4ee5\u5728\u65e9\u671f\u68c0\u6d4b\u51fa\u4f4eC<sub>t<\/sub>\uff08\u5224\u65ad\u9608\u503c\uff09\u7684\u5f3a\u9633\u6027\u6837\u672c\uff0c3\uff09\u8be5\u6280\u672f\u8fd8\u53ef\u7528\u4e8e\u591a\u9776\u6807\u75c5\u6bd2\u7684\u9776\u5411\u68c0\u6d4b\uff0c\u5176\u5728SARS-CoV-2\u53d8\u79cd\u8d8a\u6765\u8d8a\u591a\u7684\u6761\u4ef6\u4e0b\uff0c\u5177\u6709\u91cd\u8981\u610f\u4e49\u3002\u603b\u800c\u8a00\u4e4b\uff0c\u8be5\u5de5\u4f5c\u6240\u505a\u7684\u52aa\u529b\u6781\u5927\u7684\u4fc3\u8fdb\u4e86\u5e8a\u65c1\u68c0\u6d4bPCR\u6280\u672f\u7684\u53d1\u5c55\uff0c\u5e76\u4e3a\u5f53\u4e0bCOVID-19\u7684\u5feb\u901f\u68c0\u6d4b\u5e26\u6765\u4e86\u65b0\u7684\u89e3\u51b3\u65b9\u6848\u3002<\/p>\n<p>\u6587\u732e\u94fe\u63a5\uff1a<\/p>\n<p>DOI\uff1a10.1038\/s41565-022-01175-4<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u3010\u5bfc\u8bfb\u3011 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