{"id":240426,"date":"2022-07-30T07:22:34","date_gmt":"2022-07-29T23:22:34","guid":{"rendered":"\/\/m.iemloyee.com\/?p=240426"},"modified":"2022-07-29T16:38:17","modified_gmt":"2022-07-29T08:38:17","slug":"%e5%8d%97%e4%ba%ac%e5%a4%a7%e5%ad%a6%e9%bb%84%e7%a1%95%e6%9c%80%e6%96%b0nat-nanotechnol-%ef%bc%9a%e5%88%a9%e7%94%a8%e5%b7%a5%e7%a8%8b%e5%8c%96%e7%9a%84%e7%ba%b3%e7%b1%b3%e5%ad%94%e9%89%b4%e5%ae%9a","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=240426","title":{"rendered":"\u5357\u4eac\u5927\u5b66\u9ec4\u7855\u6700\u65b0Nat. Nanotechnol.\uff1a\u5229\u7528\u5de5\u7a0b\u5316\u7684\u7eb3\u7c73\u5b54\u9274\u5b9a\u6838\u82f7\u5355\u78f7\u9178\u53ca\u5176\u8868\u89c2\u9057\u4f20\u4fee\u9970"},"content":{"rendered":"<p>\u4e00\u3001\u3010\u5bfc\u8bfb\u3011<\/p>\n<p>RNA\u4fee\u9970\u5728\u5404\u79cd\u751f\u7269\u5b66\u8fc7\u7a0b\u7684\u8c03\u63a7\u4e2d\u8d77\u7740\u81f3\u5173\u91cd\u8981\u7684\u4f5c\u7528\uff0c\u5e76\u4e0e\u8bb8\u591a\u4eba\u7c7b\u75be\u75c5\u76f8\u5173\u8054\u3002\u7136\u800c\uff0c\u76f4\u63a5\u901a\u8fc7\u6d4b\u5e8f\u9274\u5b9aRNA\u7684\u4fee\u9970\u4ecd\u7136\u5177\u6709\u6311\u6218\u6027\u3002\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u5f88\u6709\u524d\u9014\uff0c\u4f46\u76ee\u524d\u7684\u7b56\u7565\u56e0\u5e8f\u5217\u89e3\u7801\u800c\u590d\u6742\u5316\u3002\u9176\u5207\u6838\u82f7\u5355\u78f7\u9178\u7684\u5e8f\u5217\u7eb3\u7c73\u5b54\u9274\u5b9a\u53ef\u4ee5\u540c\u65f6\u63d0\u4f9b\u51c6\u786e\u7684\u5e8f\u5217\u548c\u4fee\u9970\u4fe1\u606f\u3002<\/p>\n<p><strong><b>\u4e8c\u3001\u3010\u6210\u679c\u63a0\u5f71\u3011<\/b><\/strong><\/p>\n<p><strong><b>\u5357\u4eac\u5927\u5b66\u9ec4\u7855\u8bfe\u9898\u7ec4\u5c55\u793a\u4e86\u4e00\u79cd\u82ef\u787c\u9178<\/b><\/strong><strong><b>(P<\/b><\/strong><strong><b>BA<\/b><\/strong><strong><b>)<\/b><\/strong><strong><b>\u4fee\u9970\u7684\u5f02\u516b\u805a\u4f53\u803b\u57a2\u5206\u679d\u6746\u83cc\u5b54\u86cb\u767dA\u7eb3\u7c73\u5b54<\/b><\/strong><strong><b>\u00a0<\/b><\/strong><strong><b>(M<\/b><\/strong><strong><b>spA<\/b><\/strong><strong><b>)<\/b><\/strong>\uff0c\u901a\u8fc7\u8be5\u7eb3\u7c73\u5b54\u53ef\u4ee5\u76f4\u63a5\u533a\u5206\u7ecf\u5178\u6838\u82f7\u30015-\u7532\u57fa\u80de\u82f7\u3001N6-\u7532\u57fa\u817a\u82f7\u3001N7-\u7532\u57fa\u9e1f\u82f7\u3001N1-\u7532\u57fa\u817a\u82f7\u3001\u808c\u82f7\u3001\u5047\u5c3f\u82f7\u548c\u4e8c\u6c22\u5c3f\u82f7\u7684\u5355\u78f7\u9178\u76d0\u3002\u673a\u5668\u5b66\u4e60\u51c6\u786e\u7387\u9ad8\u8fbe0.996\u3002\u8be5\u65b9\u6cd5\u4e5f\u88ab\u5e94\u7528\u4e8emicroRNA\u548c\u5929\u7136tRNA\u4fee\u9970\u7684\u5b9a\u91cf\u5206\u6790\u3002\u5b83\u9002\u7528\u4e8e\u68c0\u6d4b\u5404\u79cd\u5176\u4ed6\u6838\u82f7\u6216\u6838\u82f7\u9178\u884d\u751f\u7269\uff0c\u5e76\u53ef\u80fd\u4e3aRNA\u8868\u89c2\u9057\u4f20\u6d4b\u5e8f\u5e26\u6765\u65b0\u7684\u89c1\u89e3\u3002\u76f8\u5173\u8bba\u6587\u4ee5\u9898\u4e3a\uff1a\u201c<em><i>Identification of nucleoside monophosphates and their epigenetic modifications using an engineered nanopore<\/i><\/em>\u201d\u53d1\u8868\u5728<em><i>Nature Nanotechnology<\/i><\/em>\u4e0a\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-240427\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/07\/\u56fe\u72471-4.png\" alt=\"\" width=\"744\" height=\"325\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/07\/\u56fe\u72471-4.png 744w, \/\/m.iemloyee.com\/wp-content\/uploads\/2022\/07\/\u56fe\u72471-4-300x131.png 300w\" sizes=\"(max-width: 744px) 100vw, 744px\" \/><\/p>\n<p><strong><b>\u4e09\u3001\u3010\u6838\u5fc3\u521b\u65b0\u70b9\u3011<\/b><\/strong><\/p>\n<ul>\n<li><b><\/b><strong><b>\u672c\u5de5\u4f5c<\/b><\/strong><strong><b>\u8868\u660e\uff0c<\/b><\/strong><strong><b>\u4ee5\u5916\u5207\u6d4b\u5e8f\u65b9\u5f0f\u5bf9RNA\u8fdb\u884c\u6d4b\u5e8f\u662f\u4e00\u79cd\u4e0d\u540c\u7684\u7b56\u7565\uff0c\u8be5\u7b56\u7565\u53ef\u4ee5\u901a\u8fc7\u7eb3\u7c73\u5b54\u987a\u5e8f\u8bfb\u53d6\u5916\u5207\u6838\u9178\u9176\u5206\u89e3\u7684\u6838\u82f7\u9178<\/b><\/strong>\u3002\u7136\u800c\uff0c\u8fd9\u9700\u8981\u5b58\u5728\u80fd\u591f\u660e\u786e\u8bc6\u522b\u6240\u6709\u6838\u82f7\u9178\u53ca\u5176\u4e3b\u8981\u4fee\u9970\u7684\u9ad8\u5206\u8fa8\u7387\u7eb3\u7c73\u5b54\u3002<\/li>\n<li>\u672c\u5de5\u4f5c\u901a\u8fc7\u62a5\u544a\u7cbe\u5ea6\u4e3a996\u7684\u81ea\u5b9a\u4e49\u673a\u5668\u5b66\u4e60\u7b97\u6cd5\u5bf9microRNA\u548c\u5929\u7136tRNA\u4fee\u9970\u8fdb\u884c\u5b9a\u91cf\u5206\u6790\u3002\u5b83\u9002\u7528\u4e8e\u68c0\u6d4b\u5404\u79cd\u5176\u4ed6\u6838\u82f7\u6216\u6838\u82f7\u9178\u884d\u751f\u7269\uff0c\u5e76\u53ef\u80fd\u4e3aRNA\u8868\u89c2\u9057\u4f20\u6d4b\u5e8f\u5e26\u6765\u65b0\u7684\u89c1\u89e3\u3002<\/li>\n<li><b><\/b><strong><b>\u672c\u5de5\u4f5c<\/b><\/strong><strong><b>\u8868\u660e\uff0c<\/b><\/strong><strong><b>\u8be5\u7b56\u7565\u539f\u5219\u4e0a<\/b><\/strong><strong><b>\u8fd8<\/b><\/strong><strong><b>\u9002\u7528\u4e8e\u68c0\u6d4b\u4e8c\u78f7\u9178\u6838\u82f7\u3001\u4e09\u78f7\u9178\u6838\u82f7\u3001\u5176\u4ed6\u6838\u82f7\u9178\u4fee\u9970\u3001\u6838\u82f7\u9178\u7cd6\u548c\u6838\u82f7\u836f\u7269<\/b><\/strong><strong><b>\uff0c\u53ea\u8981\u5176\u7ed3\u6784\u4e0a\u4ecd\u4fdd\u7559\u6838\u7cd6\u7684\u987a\u5f0f\u4e8c\u9187<\/b><\/strong>\u3002<\/li>\n<\/ul>\n<p style=\"text-align: center;\"><strong><b>\u56db\u3001\u3010\u6570\u636e\u6982\u89c8\u3011<\/b><\/strong><\/p>\n<ul>\n<li><b><\/b><strong><b>\u4f7f\u7528PBA\u4fee\u9970\u7684MspA\u8bc6\u522bNMP<\/b><\/strong><\/li>\n<\/ul>\n<p>\u4e3a\u4e86\u6784\u5efa\u5f02\u516b\u805a\u4f53MspA\uff0c\u672c\u5de5\u4f5c\u5206\u522b\u5b9a\u5236\u5408\u6210\u4e86\u7f16\u7801N90C MspA-H6\u548cM2\u00a0MspA-D16H6\u7684\u4e24\u4e2a\u57fa\u56e0\u3002 \u4e24\u4e2a\u57fa\u56e0\u540c\u65f6\u63d2\u5165 pETDuet-1\u5171\u8868\u8fbe\u8f7d\u4f53\u3002\u7531\u4e00\u4e2a\u5355\u5143\u7684N90C\u00a0MspA-H6\u548c\u4e03\u4e2a\u5355\u5143\u7684M2\u00a0MspA-D16H6\u7ec4\u6210\u7684\u5f02\u516b\u805a\u4f53MspA\u662f\u552f\u4e00\u9700\u8981\u7684MspA\u7ec4\u88c5\u4f53\uff0c\u7b80\u79f0(N90C)1(M2)7 (\u56fe1a)\u3002(N90C)1(M2)7\u4e0e\u5176\u4ed6MspA\u5f02\u6784\u4f53\u7ecf\u9ad8\u5206\u8fa8\u51dd\u80f6\u7535\u6cf3\u5206\u79bb\u540e\uff0c\u8fdb\u884c\u51dd\u80f6\u63d0\u53d6\u3002\u968f\u540e\uff0c3-(\u9a6c\u6765\u9170\u4e9a\u80fa)\u82ef\u57fa\u787c\u9178(MPBA)\u4e0e(N90C)1(M2)7\u7684\u552f\u4e00\u534a\u80f1\u6c28\u9178\u53cd\u5e94(\u56fe1b)\u3002\u57281.5\u2009M KCl\uff0c10\u2009mM MOPS\uff0cpH7.0\u7f13\u51b2\u6db2\u4e2d\uff0c\u901a\u8fc7\u5355\u901a\u9053\u8bb0\u5f55\uff0c\u5728\u5355\u5206\u5b50\u6c34\u5e73\u4e0a\u5bf9\u8be5\u53cd\u5e94\u8fdb\u884c\u4e86\u5b9e\u65f6\u89c2\u5bdf(\u56fe1c)\u3002NMPs\u7531\u6838\u7cd6\u3001\u78f7\u9178\u57fa\u56e2\u548c\u6838\u57fa\u7ec4\u6210\uff0c\u4f5c\u4e3aRNA\u7684\u5355\u4f53\u5355\u4f4d\u3002\u7531\u4e8e\u6838\u7cd6\u4e2d\u5b58\u5728\u987a\u5f0f\u4e8c\u9187\uff0cNMPs\u4e0ePBA\u5177\u6709\u4eb2\u548c\u529b\uff0c\u53ef\u80fd\u76f4\u63a5\u88abMspA-PBA\u68c0\u6d4b\u5230\u3002\u7ee7\u7eed\u65bd\u52a0+200 mV\u7684\u8de8\u819c\u7535\u4f4d\u3002\u4ee5\u817a\u560c\u5464\u5355\u6838\u82f7\u9178(AMP)\u3001\u9e1f\u560c\u5464\u5355\u6838\u82f7\u9178(GMP)\u3001\u80de\u5627\u5576\u5355\u6838\u82f7\u9178(CMP)\u548c\u5c3f\u5627\u5576\u5355\u6838\u82f7\u9178(UMP)\u56db\u79cd\u5178\u578b\u7684NMP\u4e3a\u5206\u6790\u7269(\u56fe1d)\u3002\u7acb\u5373\u89c2\u5bdf\u5230\u7531NMPs\u5f15\u8d77\u7684\u8fde\u7eed\u7535\u963b\u8109\u51b2(\u56fe1d)\u3002\u7136\u800c\uff0cM2 MspA\u6d4b\u8bd5\u65f6\u6ca1\u6709\u89c2\u5bdf\u5230\u4efb\u4f55\u4e8b\u4ef6\uff0c\u8bc1\u5b9e\u4f4d\u4e8e\u5b54\u9699\u6536\u7f29\u5904\u7684PBA\u5728NMP\u4f20\u611f\u4e8b\u4ef6\u7684\u4ea7\u751f\u4e2d\u81f3\u5173\u91cd\u8981\u3002\u4f7f\u7528MspA-PBA\uff0c\u8131\u6c27\u6838\u82f7\u5355\u78f7\u9178(dNMP)\u65e0\u6cd5\u62a5\u544a\u4efb\u4f55\u4e8b\u4ef6\u3002\u8fd9\u662f\u9884\u671f\u7684\uff0c\u56e0\u4e3adNMPs\u6ca1\u6709\u4f20\u611f\u6240\u5fc5\u9700\u7684\u987a\u4e8c\u9187\u7ed3\u6784\u3002<strong><b>\u672c\u5de5\u4f5c\u8fd8\u5229\u7528MspA-PBA\u5bf9CMP\u3001UMP\u3001AMP\u548cGMP\u8fdb\u884c\u4e86\u540c\u65f6\u4f20\u611f(\u56fe1f)\uff0c\u6839\u636e\u4e0d\u540c\u7684\u963b\u585e\u7279\u6027\u53ef\u4ee5\u76f4\u63a5\u9274\u5b9a\u4e0d\u540c\u7684NMP\u8eab\u4efd<\/b><\/strong>\u3002\u636e\u6211\u4eec\u6240\u77e5\uff0c\u5728\u4e8b\u4ef6\u5206\u5e03\u4e0a\u6ca1\u6709\u4efb\u4f55\u91cd\u53e0\u7684\u5178\u578bNMP\u4e4b\u95f4\u7684\u7eb3\u7c73\u5b54\u533a\u5206\u5ea6\u4ee5\u524d\u4ece\u672a\u6709\u8fc7\u62a5\u9053\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-240428\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/07\/\u56fe\u72472-4.png\" alt=\"\" width=\"652\" height=\"594\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/07\/\u56fe\u72472-4.png 652w, \/\/m.iemloyee.com\/wp-content\/uploads\/2022\/07\/\u56fe\u72472-4-300x273.png 300w\" sizes=\"(max-width: 652px) 100vw, 652px\" \/><\/p>\n<p>\u56fe1. \u4f7f\u7528PBA\u4fee\u9970\u7684MspA\u533a\u5206\u7ecf\u5178\u7684NMP\u00a9 2022 Springer Nature Limited<\/p>\n<ul>\n<li><b><\/b><strong><b>\u533a\u5206\u8868\u89c2\u9057\u4f20NMP<\/b><\/strong><\/li>\n<\/ul>\n<p>\u636e\u6587\u732e\u62a5\u9053\uff0c~170\u4e2a\u8868\u89c2\u9057\u4f20\u5b66NMPs\u65e9\u5df2\u88ab\u53d1\u73b0\u3002\u8fd9\u4e9b\u8868\u89c2\u9057\u4f20NMPs\u5177\u6709\u6781\u5176\u5fae\u5c0f\u7684\u7ed3\u6784\u5dee\u5f02\uff0c\u5bf9\u76f4\u63a5\u9274\u5b9a\u63d0\u51fa\u4e86\u6781\u5927\u7684\u6311\u6218\u3002\u5f53\u8868\u89c2\u9057\u4f20NMPs\u4e0eMspA-PBA\u7ed3\u5408\u65f6\uff0c\u53ef\u4ee5\u901a\u8fc7\u76f4\u63a5\u76d1\u6d4b\u7eb3\u7c73\u5b54\u8bfb\u51fa\u7684\u4e8b\u4ef6\u7279\u5f81\u6765\u89e3\u51b3\u8fd9\u4e00\u6311\u6218\u3002\u8fd9\u91cc\u6d4b\u8bd5\u4e867\u4e2d\u4e3b\u8981\u7684\u8868\u89c2\u9057\u4f20NMPs\uff0c \u4ed6\u4eec\u6db5\u76d6\u4e86\u7532\u57fa\u5316\u3001\u8131\u6c28\u3001\u5f02\u6784\u5316\u548c\u8fd8\u539f\u7b49\u4fee\u9970\u7c7b\u578b\u3002\u5b83\u4eec\u7684\u78b1\u57fa\u7ec4\u6210\u5982\u56fe2a\u6240\u793a\u3002\u8868\u89c2\u9057\u4f20NMPs\u4e8b\u4ef6\u5177\u6709\u660e\u663e\u4e0d\u540c\u7684\u963b\u65ad\u5e45\u5ea6\u3002\u4e3a\u4e86\u5145\u5206\u5bf9\u6bd4\u76ee\u524d\u6d4b\u8bd5\u7684\u6240\u6709NMP\uff0c\u5728\u5c0f\u63d0\u7434\u56fe\u4e2d\u663e\u793a\u4e86\u6bcf\u4e2aNMP\u7684%Ib\u5206\u5e03\uff0c\u8bf4\u660e\u51e0\u4e4e\u6240\u6709\u7684NMP\u90fd\u5df2\u7ecf\u5b8c\u5168\u53ef\u4ee5\u901a\u8fc7\u5206\u6790\u5176%Ib\u6765\u533a\u5206\uff0c\u5c3d\u7ba1UMP\u548cm5C\u7684\u4e8b\u4ef6\u5206\u5e03\u4ecd\u7136\u5b58\u5728\u4e00\u5b9a\u7684\u91cd\u53e0(\u56fe2b)\u3002\u901a\u8fc7\u7ed8\u5236\u5305\u542b11\u4e2a\u4e0d\u540c\u5206\u6790\u7269NMP\u7ed3\u5408\u4e8b\u4ef6\u7684% Ib\u4e0eSD\u7684\u6563\u70b9\u56fe\uff0c\u751f\u621011\u4e2a\u5b8c\u5168\u72ec\u7acb\u7684\u4e8b\u4ef6\u79cd\u7fa4 (\u56fe2c)\u3002\u8fd9\u8bc1\u5b9e\u4e86\u8fd9\u79cd\u68c0\u6d4b\u6a21\u5f0f\u53ef\u4ee5\u5e94\u7528\u4e8e\u8868\u89c2\u9057\u4f20NMPs\u3002\u7136\u800c\uff0c\u4ee5\u524d\u4ece\u672a\u62a5\u9053\u8fc7\u5229\u7528\u7eb3\u7c73\u5b54\u540c\u65f6\u76f4\u63a5\u533a\u5206\u5982\u6b64\u591a\u7684\u6838\u82f7\u9178\u4fee\u9970\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-240429\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/07\/\u56fe\u72473-4.png\" alt=\"\" width=\"654\" height=\"472\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/07\/\u56fe\u72473-4.png 654w, \/\/m.iemloyee.com\/wp-content\/uploads\/2022\/07\/\u56fe\u72473-4-300x217.png 300w\" sizes=\"(max-width: 654px) 100vw, 654px\" \/><\/p>\n<p>\u56fe2. MspA-PBA\u9274\u5b9a\u7684\u8868\u89c2\u9057\u4f20NMP\u00a9 2022 Springer Nature Limited<\/p>\n<ul>\n<li><b><\/b><strong><b>\u901a\u8fc7\u673a\u5668\u5b66\u4e60\u8bc6\u522bNMP<\/b><\/strong><\/li>\n<\/ul>\n<p><strong><b>\u672c\u5de5\u4f5c<\/b><\/strong><strong><b>\u5efa\u7acb\u4e86\u4e00\u79cd\u673a\u5668\u5b66\u4e60\u7b97\u6cd5\u6765\u81ea\u52a8\u8bc6\u522bNMPs<\/b><\/strong>\u3002\u6574\u4e2a\u8bad\u7ec3\u8fc7\u7a0b\u5305\u62ec\u6570\u636e\u96c6\u8f93\u5165\u3001\u7279\u5f81\u63d0\u53d6\u548c\u6a21\u578b\u5efa\u7acb(\u56fe3a)\u3002\u6570\u636e\u96c6\u4e2d\u7684\u6240\u6709\u4e8b\u4ef6\u90fd\u6709\u5df2\u77e5\u7684\u6807\u7b7e\u3002\u5229\u7528MATLAB\u81ea\u52a8\u63d0\u53d6\u6bcf\u4e2a\u4e8b\u4ef6\u7684%Ib\u548cSD\uff0c\u5f62\u6210\u7279\u5f81\u77e9\u9635\u3002\u5bf9\u4e3b\u6d41\u6a21\u578b\u8fdb\u884c\u4e86\u8bc4\u4f30\uff0c\u5b83\u4eec\u90fd\u663e\u793a\u4e86\u4ee4\u4eba\u6ee1\u610f\u7684\u9a8c\u8bc1\u7cbe\u5ea6\uff0c\u8868\u660e\u8f93\u5165\u6570\u636e\u8d28\u91cf\u8f83\u9ad8\u3002\u5177\u4f53\u6765\u8bf4\uff0c\u6734\u7d20\u8d1d\u53f6\u65af\u6a21\u578b\u548c\u7ebf\u6027\u652f\u6301\u5411\u91cf\u673a(SVM)\u6a21\u578b\u7684\u6700\u9ad8\u51c6\u786e\u7387\u5f97\u5206\u4e3a0.996\u3002\u7efc\u5408\u8003\u8651\u6d4b\u8bd5\u96c6\u7cbe\u5ea6\uff0c\u7ebf\u6027SVM\u6a21\u578b\u8868\u73b0\u6700\u4f18\u3002\u57fa\u4e8e\u7ebf\u6027SVM\u6a21\u578b\u6d4b\u8bd5\u96c6\u7684\u6df7\u6dc6\u77e9\u9635\u7ed3\u679c\u5982\u56fe3b\u6240\u793a\uff0c\u5176\u4e2d\u5927\u90e8\u5206NMP\u68c0\u6d4b\u7ed3\u679c\u7684\u51c6\u786e\u7387\u4e3a99%\u6216100%\u3002\u56fe3c\u4e2d\u8fd8\u5c55\u793a\u4e86\u7ebf\u6027SVM\u6a21\u578b\u751f\u6210\u7684\u51b3\u7b56\u8fb9\u754c\u56fe\u3002\u4e3a\u4e86\u4ece\u6df7\u5408\u7269\u4e2d\u663e\u793a\u4e8b\u4ef6\u6807\u8bc6\uff0c\u56fe3d\u5c55\u793a\u4e86\u5305\u542b\u6765\u81ea11\u4e2a\u4e0d\u540cNMP\u7684\u4e8b\u4ef6\u7684\u4ee3\u8868\u6027\u7535\u6d41\u66f2\u7ebf\u3002\u4ece\u4e2d\u53ef\u4ee5\u8bc6\u522b\u4e0d\u540c\u7684NMP\u7c7b\u578b\uff0c\u5e76\u5728\u66f2\u7ebf\u7684\u4e0a\u65b9\u6807\u6ce8\u673a\u5668\u5b66\u4e60\u9884\u6d4b\u7684\u76f8\u5e94\u6807\u7b7e\u3002\u8fd9\u6709\u6548\u7684\u5e2e\u52a9\u4e86<strong><b>\u5b9e\u9645\u6d4b\u91cf\u573a\u666f\u4e2d\u4e0d\u540cNMP\u7684\u81ea\u52a8\u7eb3\u7c73\u5b54<\/b><\/strong><strong><b>\u8bc6\u522b<\/b><\/strong>\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-240430\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/07\/\u56fe\u72474-4.png\" alt=\"\" width=\"771\" height=\"392\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/07\/\u56fe\u72474-4.png 771w, \/\/m.iemloyee.com\/wp-content\/uploads\/2022\/07\/\u56fe\u72474-4-300x153.png 300w, \/\/m.iemloyee.com\/wp-content\/uploads\/2022\/07\/\u56fe\u72474-4-768x390.png 768w\" sizes=\"(max-width: 771px) 100vw, 771px\" \/><\/p>\n<p>\u56fe3. \u673a\u5668\u5b66\u4e60\u8f85\u52a9NMP\u8bc6\u522b\u00a9 2022 Springer Nature Limited<\/p>\n<ul>\n<li><b><\/b><strong><b>\u4ece<\/b><\/strong><strong><b>\u7532\u57fa\u5316microRNA<\/b><\/strong><strong><b>\u4e2d\u68c0\u6d4b<\/b><\/strong><strong><b>\u8868\u89c2\u9057\u4f20NMP<\/b><\/strong><\/li>\n<\/ul>\n<p>\u6211\u4eec\u8fdb\u4e00\u6b65\u8bd5\u56fe\u5bf9RNA\u4e2d\u8868\u89c2\u9057\u4f20NMP\u76f4\u63a5\u68c0\u6d4b\uff08\u56fe4a\uff09\u3002\u901a\u8fc7S1\u6838\u9178\u9176\u5904\u7406\uff0cRNA\u9996\u5148\u88ab\u9176\u5206\u89e3\u6210NMP\uff0c\u7136\u540e\u88abMspA-PBA\u4f20\u611f\u3002\u89c2\u5bdf\u5230\u7684\u7eb3\u7c73\u5b54\u4e8b\u4ef6\u7531\u5148\u524d\u8bad\u7ec3\u7684\u673a\u5668\u5b66\u4e60\u6a21\u578b\u8bc6\u522b\u3002\u5e94\u7528\u4e86\u4e24\u79cd\u5df2\u77e5\u7532\u57fa\u5316\u4f4d\u70b9\u7684microRNA\uff0c\u5305\u62echsa-miR-21\u548chsa-miR-17\u3002\u5177\u4f53\u800c\u8a00\uff0chsa-miR-21\u5728\u4f4d\u7f6e9\u5904\u542b\u6709m5C\uff0chsa-miR-17\u5728\u4f4d\u7f6e13\u5904\u542b\u6709m6C\u3002<strong><b>\u6ca1\u6709\u4efb\u4f55\u9176\u5904\u7406\uff0chsa-miR-21\u548chsa-miR-17\u88abMspA-PBA<\/b><\/strong>\u4f20\u611f\u65f6\u4ec5\u89c2\u5bdf\u5230\u5177\u6709\u6df1\u6d45\u4e0d\u4e00\u632f\u5e45\u7684\u6bdb\u523a\u4e8b\u4ef6\uff0c\u8868\u660e\u8fd9\u79cd\u4f20\u611f\u673a\u5236\u5bf9\u6a21\u677fRNA\u672c\u8eab\u4e0d\u654f\u611f\u3002\u4e3a\u51cf\u5c11\u7518\u6cb9\u5bf9S1\u6838\u9178\u9176\u539f\u6db2\u7684\u5e72\u6270\uff0c\u91c7\u7528\u8d85\u6ee4\u6cd5\u5bf9S1\u6838\u9178\u9176\u8fdb\u884c\u9884\u5904\u7406\uff0c\u53bb\u9664\u7518\u6cb9\u3002\u7136\u540e\u7528\u9884\u5904\u7406\u540e\u7684S1\u6838\u9178\u9176\u572823\u2103\u4e0b\u6d88\u5316microRNA 4\u2009h \u3002<strong><b>\u4ece\u51dd\u80f6\u7535\u6cf3\u7ed3\u679c\u6765\u770b\uff0c\u4e24\u79cdmicroRNA\u5747\u88ab\u5f7b\u5e95\u5206\u89e3<\/b><\/strong>\u3002\u9176\u5904\u7406\u4ea7\u7269\u7ecf\u8d85\u6ee4\u9664\u53bbS1\u6838\u9178\u9176\u3002\u5728\u7eb3\u7c73\u5b54\u68c0\u6d4b\u4e2d\uff0c\u5c06hsa-miR-21\u9176\u5207\u4ea7\u7269\u52a0\u5165cis\u4e2d\uff0c\u7ec8\u6d53\u5ea6\u4e3a100 ng \u03bcl<sup>-1<\/sup>\u3002\u56fe4b\u663e\u793a\u4e86\u4e00\u4e2a\u5177\u6709\u4ee3\u8868\u6027\u7684\u7535\u6d41\u66f2\u7ebf\uff0c\u5176\u4e2d\u89c2\u5bdf\u5230\u8bb8\u591aNMP\u7ed3\u5408\u4e8b\u4ef6\uff0c<strong><b>\u8868\u660eMspA-PBA\u5f88\u597d\u5730\u68c0\u6d4b\u5230\u4e86\u751f\u6210\u7684NMP<\/b><\/strong>\u3002\u4f7f\u7528\u673a\u5668\u5b66\u4e60\u7b97\u6cd5\u5bf9\u4e8b\u4ef6\u8fdb\u884c\u8bc6\u522b\uff0c\u6240\u83b7\u5f97\u7684NMP\u7ec4\u6210\u4e0e\u5e8f\u5217\u5b8c\u5168\u4e00\u81f4\u3002\u4e3a\u4e86\u68c0\u9a8c\u5176\u901a\u7528\u6027\uff0chsa-miR-17\u4e5fhas\u8fdb\u884c\u4e86\u76f8\u540c\u7684\u68c0\u9a8c\u3002\u56fe4d\u5c55\u793a\u4e86hsa-miR-17\u9176\u5207\u4ea7\u7269\u4e2d\u5177\u6709\u4ee3\u8868\u6027\u7684\u4f20\u611f\u4e8b\u4ef6\u3002\u6563\u70b9\u56fe\u7ed3\u679c\u663e\u793a5\u4e2aNMP\u4e8b\u4ef6\u5206\u5e03\uff0c\u5206\u522b\u5bf9\u5e94CMP\u3001UMP\u3001AMP\u3001GMP\u548cm6A (\u56fe4e)\uff0c\u4e0ehsa-miR-17\u7684\u5e8f\u5217\u7ec4\u6210\u4e00\u81f4\u3002<strong><b>\u5b9a\u91cf\u5206\u6790\u663e\u793a\uff0cm6A\u4f4d\u70b9\u4e2a\u6570\u4e3a1.08\uff0c\u8868\u660ehsa-miR-17\u4e2d\u4ec5\u5b58\u57281\u4e2am6A\u4f4d\u70b9\uff0c\u4e5f\u4e0e\u9884\u671f\u76f8\u7b26<\/b><\/strong>\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-240431\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/07\/\u56fe\u72475-4.png\" alt=\"\" width=\"778\" height=\"439\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/07\/\u56fe\u72475-4.png 778w, \/\/m.iemloyee.com\/wp-content\/uploads\/2022\/07\/\u56fe\u72475-4-300x169.png 300w, \/\/m.iemloyee.com\/wp-content\/uploads\/2022\/07\/\u56fe\u72475-4-768x433.png 768w\" sizes=\"(max-width: 778px) 100vw, 778px\" \/><\/p>\n<p>\u56fe4. \u68c0\u6d4bRNA\u7684\u8868\u89c2\u9057\u4f20\u4fee\u9970\u00a9 2022 Springer Nature Limited<\/p>\n<ul>\n<li><b><\/b><strong><b>\u4ece\u9175\u6bcdtRNAPhe\u4e2d\u68c0\u6d4b\u8868\u89c2\u9057\u4f20NMP<\/b><\/strong><\/li>\n<\/ul>\n<p>\u8f6c\u8fd0RNA (tRNA)\u662f\u4e00\u79cd\u4f4e\u5206\u5b50\u91cfRNA\uff0c\u7528\u4e8e\u8fde\u63a5mRNA\u5e8f\u5217\u548c\u6c28\u57fa\u9178\u5e8f\u5217\u3002\u6210\u719f\u7684tRNA\u8fd8\u5305\u542b\u4e30\u5bcc\u7684\u5316\u5b66\u4fee\u9970\u3002\u636e\u62a5\u9053\uff0c\u5df2\u5728tRNA\u4e2d\u53d1\u73b0\u4e8690\u591a\u79cd\u7c7b\u578b\u7684\u4fee\u9970\u3002\u56e0\u6b64\uff0c\u5b83\u662f\u8bc4\u4f30MspA-PBA\u5728\u9274\u5b9a\u5929\u7136\u6837\u54c1\u8868\u89c2\u9057\u4f20\u4fee\u9970\u4e2d\u6027\u80fd\u7684\u7406\u60f3RNA\u3002\u5564\u9152\u9175\u6bcd\u82ef\u4e19\u6c28\u9178\u7279\u5f02\u6027 tRNA(\u9175\u6bcdtRNA<sup>Phe<\/sup>)\u88ab\u7528\u4f5c\u6a21\u578bRNA\u4ee5\u6d4b\u8bd5\u5176\u53ef\u884c\u6027\u3002MspA-PBA\u539f\u5219\u4e0a\u53ef\u68c0\u6d4b\u5230m2G,m<sup>2<\/sup><sub>2<\/sub>G\u3001T\u548cY\u7684\u5355\u78f7\u9178\u916f\uff0c\u6709\u671b\u89c2\u5bdf\u5230\u65b0\u7684\u4e8b\u4ef6\u7c07\u3002\u4f46\u662f\uff0c\u7531\u4e8e\u7f3a\u4e4f\u76f8\u5e94\u7684\u7eaf\u5316\u5408\u7269\u6765\u4ea7\u751f\u7528\u4e8e\u8bad\u7ec3\u7684\u4e8b\u4ef6\uff0c\u76f8\u5e94\u7684\u7eb3\u7c73\u5b54\u4e8b\u4ef6\u662f\u53ef\u68c0\u6d4b\u7684\u4f46\u65e0\u6cd5\u8bc6\u522b\u7684\u3002\u7f3a\u5c11\u987a\u5f0f\u4e8c\u5143\u9187\u7684Cm\u548cGm\u539f\u5219\u4e0a\u662fMspA-PBA\u65e0\u6cd5\u68c0\u6d4b\u7684\u3002tRNAPhe\u9996\u5148\u572823\u00b0C\u4e0b\u7528S1\u6838\u9178\u9176\u5904\u740615 h\uff0c\u5f97\u5230NMPs\u3002\u6839\u636e\u51dd\u80f6\u7535\u6cf3\u7ed3\u679c\uff0c\u8bc1\u5b9etRNAPhe\u5df2\u88ab\u5f7b\u5e95\u5206\u89e3(\u56fe5b)\u3002\u5c06\u9176\u5904\u7406\u4ea7\u7269\u8d85\u6ee4\u819c\u53bb\u9664S1\u6838\u9178\u9176\uff0c\u5e76\u7528\u4e8e\u540e\u7eed\u7684\u7eb3\u7c73\u5b54\u68c0\u6d4b\u3002<\/p>\n<p>\u9175\u6bcdtRNA<sup>Phe<\/sup>\u7684\u4fee\u9970\u56fe\u8c31\u7ed3\u679c\u5982\u56fe5c\u6240\u793a\u3002\u6210\u529f\u68c0\u6d4b\u51faD\u3001\u03c8\u3001m<sup>5<\/sup>C\u3001m<sup>7<\/sup>G\u548cm<sup>1<\/sup>A\uff0c\u4e0e\u524d\u671f\u8bad\u7ec3\u7ed3\u679c\u548c\u6587\u732e\u62a5\u9053\u4e00\u81f4\u3002\u89c2\u5bdf\u5230\u5c11\u91cfm6A\u4e8b\u4ef6\uff0c\u53ef\u80fd\u662f\u6765\u81ea\u80cc\u666f\u4e8b\u4ef6\u3002\u5b9a\u91cf\u5206\u6790\u8868\u660e\uff0c\u9175\u6bcdtRNA<sup>Phe<\/sup>\u4e2dNMP\u76f8\u5bf9\u7ec4\u6210\u4e3a17.53 GMP\u300116.36 AMP\u300116.19 CMP\u300112.06 UMP\u30013.24\u03c8\u30012.17 D\u30011.53 m5C\u30010.40 m7G\u30010.37 m1A\u30010.11 m6A\u548c0.04 I\uff0c\u4e0e\u5b9e\u9645\u503c\u76f8\u7b26(\u56fe5d)\u3002\u8fd8\u8fdb\u884c\u4e86\u4e09\u4e2a\u72ec\u7acb\u8bd5\u9a8c\uff0c\u5f97\u51fa\u4e86\u76f8\u540c\u7684\u7ed3\u8bba\uff0c\u8bc1\u5b9e\u4e86\u8fd9\u9879\u6280\u672f\u7684\u91cd\u590d\u6027\u3002\u9175\u6bcdtRNA<sup>Phe<\/sup>\u6d88\u5316\u4ea7\u7269\u4e2d\u542b\u6709\u4e8b\u4ef6\u7684\u4ee3\u8868\u6027\u7535\u6d41\u66f2\u7ebf\u5982\u56fe5e\u6240\u793a\u3002<strong><b>\u4e0a\u8ff0\u7ed3\u679c<\/b><\/strong><strong><b>\u5f88\u597d\u7684\u9a8c\u8bc1\u4e86<\/b><\/strong><strong><b>MspA-PBA\u4ece\u5929\u7136RNA\u4e2d<\/b><\/strong><strong><b>\u68c0\u6d4b<\/b><\/strong><strong><b>NMPs\u53ca\u5176\u8868\u89c2\u9057\u4f20\u4fee\u9970\u7684\u80fd\u529b<\/b><\/strong>\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-240432\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/07\/\u56fe\u72476-2.png\" alt=\"\" width=\"773\" height=\"485\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2022\/07\/\u56fe\u72476-2.png 773w, \/\/m.iemloyee.com\/wp-content\/uploads\/2022\/07\/\u56fe\u72476-2-300x188.png 300w, \/\/m.iemloyee.com\/wp-content\/uploads\/2022\/07\/\u56fe\u72476-2-768x482.png 768w\" sizes=\"(max-width: 773px) 100vw, 773px\" \/><\/p>\n<p>\u56fe5. \u9175\u6bcdtRNA<sup>Phe<\/sup>\u8868\u89c2\u9057\u4f20\u4fee\u9970\u7684\u5b9a\u91cf\u68c0\u6d4b\u00a9 2022 Springer Nature Limited<\/p>\n<p style=\"text-align: center;\"><strong><b>\u4e94\u3001\u3010\u6210\u679c\u542f\u793a\u3011<\/b><\/strong><\/p>\n<p>\u636e\u62a5\u9053\uff0c\u542b\u6709\u552f\u4e00PBA\u7684\u5f02\u516b\u805a\u4f53MspA\u80fd\u4f20\u611fNMPs\u3002\u5341\u4e00\u79cd\u7c7b\u578b\u7684NMP\u662f\u5b8c\u5168\u533a\u5206\u7684\uff0c\u6548\u679c\u8fdc\u4f18\u4e8e\u03b1-HL\u6216\u56fa\u6001\u7eb3\u7c73\u5b54\u3002\u672c\u5de5\u4f5c\u6784\u5efa\u4e86\u673a\u5668\u5b66\u4e60\u7b97\u6cd5\uff0c\u62a5\u544a\u4e860.996\u7684\u7cbe\u5ea6\u3002\u552f\u4e00\u7684\u9650\u5236\u662f\uff0c\u5f53\u524d\u7684\u4f20\u611f\u7b56\u7565\u65e0\u6cd5\u68c0\u6d4b\u6838\u7cd6\u4fee\u9970\u7684NMPs\uff0c\u5982Cm\u548cGm\u3002\u4e0e\u8d28\u8c31\u76f8\u6bd4\uff0c\u6211\u4eec\u7684\u65b9\u6cd5\u63d0\u4f9b\u4e86\u66f4\u9ad8\u7684\u5206\u8fa8\u7387\uff0c\u7279\u522b\u662f\u5728\u533a\u5206RNA\u4f4d\u7f6e\u5f02\u6784\u4f53\u65b9\u9762\u3002<strong><b>\u56e0\u6b64\uff0c\u5b83\u66f4\u9002\u7528\u4e8e\u6df7\u5408\u548c<\/b><\/strong><strong><b>\u5929\u7136<\/b><\/strong><strong><b>\u6837\u54c1\u7684RNA\u4fee\u9970\u68c0\u6d4b\uff0c\u65e0\u9700\u8026\u5408\u4efb\u4f55\u8272\u8c31\u5206\u79bb\u6280\u672f\u548c\u590d\u6742\u7684\u6570\u636e\u89e3\u91ca<\/b><\/strong>\u3002\u8be5\u4f20\u611f\u7b56\u7565\u4e5f\u88ab\u5e94\u7528\u4e8e\u9274\u5b9a\u5929\u7136RNA\u6837\u54c1\u4e2d\u9176\u5207\u7684NMPs\uff0c\u8868\u660e\u5229\u7528\u9176\u5076\u8054\u7684MspA-PBA\u8fdb\u884c\u5916\u5207\u6d4b\u5e8f\u7684\u53ef\u884c\u6027\u3002\u867d\u7136\u6ca1\u6709\u8bba\u8bc1\uff0c\u4f46\u8fd9\u79cd\u7b56\u7565\u539f\u5219\u4e0a\u9002\u7528\u4e8e\u4f20\u611f\u6838\u82f7\u4e8c\u78f7\u9178\u76d0\u7c7b\u3001\u6838\u82f7\u4e09\u78f7\u9178\u3001\u5176\u4ed6\u6838\u82f7\u9178\u4fee\u9970\u3001\u6838\u82f7\u9178\u7cd6\u548c\u6838\u82f7\u7c7b\u836f\u7269\uff0c\u53ea\u8981\u6838\u7cd6\u7684\u987a\u5f0f\u4e8c\u9187\u4ecd\u7136\u4fdd\u7559\u3002<\/p>\n<p>\u7b2c\u4e00\u4f5c\u8005\uff1a\u738b\u7389\u7434,\u00a0\u5f20\u5584\u96e8, \u8d3e\u6587\u4e1c<\/p>\n<p>\u901a\u8baf\u4f5c\u8005\uff1a\u9ec4\u7855<\/p>\n<p>\u901a\u8baf\u5355\u4f4d\uff1a\u5357\u4eac\u5927\u5b66<\/p>\n<p>\u8bba\u6587doi\uff1a<\/p>\n<p>https:\/\/doi.org\/10.1038\/s41565-022-01169-2<\/p>\n<p><strong>\u516d\u3001\u3010\u56e2\u961f\u4ecb\u7ecd\u3011<\/strong><\/p>\n<p>\u672c\u8bfe\u9898\u7ec4\u96b6\u5c5e\u4e8e\u5357\u4eac\u5927\u5b66\u5316\u5b66\u5316\u5de5\u5b66\u9662\u751f\u547d\u5206\u6790\u5316\u5b66\u56fd\u5bb6\u91cd\u70b9\u5b9e\u9a8c\u5ba4\u3001\u5357\u4eac\u5927\u5b66\u5316\u5b66\u548c\u751f\u7269\u533b\u836f\u521b\u65b0\u7814\u7a76\u9662\uff0c\u4e3b\u8981\u7814\u7a76\u65b9\u5411\u4e3a\u5355\u5206\u5b50\u751f\u7269\u4f20\u611f\u5668\u4ef6\u548c\u5355\u5206\u5b50\u751f\u7269\u7269\u7406\uff0c\u540c\u65f6\u517c\u987e\u9762\u5411\u7406\u89e3\u751f\u547d\u8fc7\u7a0b\u7684\u5316\u5b66\u672c\u8d28\u7684\u57fa\u7840\u7814\u7a76\u548c\u5177\u6709\u5b9e\u7528\u610f\u4e49\u7684\u65b0\u578b\u533b\u7597\u8bca\u65ad\u5668\u4ef6\u7684\u7814\u53d1\u3002\u8bfe\u9898\u7ec4PI\u9ec4\u7855\u6559\u6388\u957f\u671f\u4ece\u4e8b\u5355\u5206\u5b50\u751f\u7269\u7269\u7406\u548c\u5355\u5206\u5b50\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u6280\u672f\u7684\u65b0\u4eea\u5668\uff0c\u65b0\u65b9\u6cd5\u7814\u7a76\uff0c\u5148\u540e\u5e08\u4ece\u4e8eStuart Lindsay\u00a0\u6559\u6388\uff08\u4e9a\u5229\u6851\u90a3\u5dde\u7acb\u5927\u5b66\u6559\u6388\uff0cAgilent\u00a0\u516c\u53f8Molecular Imaging\u521b\u59cb\u4eba\uff09 \u548cHagan Bayley\u6559\u6388 \uff08\u725b\u6d25\u5927\u5b66\u6559\u6388\uff0c\u82f1\u56fd\u7687\u5bb6\u79d1\u5b66\u9662\u9662\u58eb\uff0cOxford Nanopore\u521b\u59cb\u4eba\uff09\u5e76\u5728Nature Nanotechnology, Angewandte Chemie International Edition, Chemical Science, Nano Letters, Journal of Physical Chemistry C, Nanotechnology\u7b49\u9ad8\u6c34\u5e73\u671f\u520a\u53d1\u8868\u591a\u7bc7\u8bba\u6587\uff0c\u5176\u4e2d\u4ee5\u7b2c\u4e00\u4f5c\u8005\u8eab\u4efd\u53d1\u8868Nature Nanotechnology\u6742\u5fd7\u4e24\u7bc7\uff0c\u5e76\u6709\u56fd\u9645\u6388\u6743\u4e13\u52292\u9879\uff0c\u5747\u8f6c\u8ba9\u4e8e\u56fd\u9645\u77e5\u540d\u4f01\u4e1a\u3002\u4e8e2015\u5e747\u6708\u52a0\u76df\u5357\u4eac\u5927\u5b66\u751f\u547d\u5206\u6790\u5316\u5b66\u56fd\u5bb6\u91cd\u70b9\u5b9e\u9a8c\u5ba4\u5f00\u5c55\u72ec\u7acb\u8bfe\u9898\u7814\u7a76\u3002\u81ea2015\u5e747\u6708\u5165\u804c\u5357\u4eac\u5927\u5b66\uff0c\u62e5\u6709\u72ec\u7acb\u5b9e\u9a8c\u5ba42\u95f4\uff0c\u7ec6\u80de\u95f41\u95f4\uff0c\u7ea6\u8ba1160\u5e73\u3002\u73b0\u6709\u535a\u58eb\u540e1\u4eba\uff0c\u535a\u58eb\u7814\u7a76\u751f8\u4eba\uff0c\u7855\u58eb\u7814\u7a76\u751f5\u4eba\uff0c\u672c\u79d1\u751f1\u4eba\uff0c\u5171\u8ba114\u540d\u5b66\u751f\uff0c\u7ecf\u8fc7\u516d\u5e74\u591a\u7684\u5b9e\u9a8c\u5ba4\u5efa\u8bbe,\u5df2\u5b8c\u6210\u5b9e\u9a8c\u5ba4\u7684\u4eea\u5668\u642d\u5efa\u53ca\u56e2\u961f\u7684\u7ec4\u5efa\u5de5\u4f5c\uff0c\u79d1\u7814\u5de5\u4f5c\u6709\u5e8f\u5f00\u5c55\u3002 \u8fd1\u4e00\u5e74\u6765\u5171\u8ba1\u53d1\u8868\u9ad8\u6c34\u5e73\u8bba\u6587\u8fbe17\u7bc7\uff0c\u5176\u4e2d\u5305\u62ecNature Nanotechnology 1\u7bc7\u3001JACS 2\u7bc7\u3001Angewandte Chemie\u00a03\u7bc7\u3001Nature Communications 2\u7bc7\u3001Nano Lett 2\u7bc7\u3001ACS Nano 1\u7bc7\u7b49\u3002\u83b7\u5f97\u56fd\u5bb6\u7ea7\u91cd\u5927\u4eba\u624d\u5de5\u7a0bA\u7c7b\uff08\u9752\u5e74\u9879\u76ee\uff09\u3001\u56fd\u5bb6\u81ea\u7136\u57fa\u91d1\u3001\u6c5f\u82cf\u7701\u201c\u53cc\u521b\u4eba\u624d\u201d\u9879\u76ee\u3001\u6c5f\u82cf\u7701\u201c\u53cc\u521b\u56e2\u961f\u201d\u9879\u76ee\u3001\u6c5f\u82cf\u7701\u201c\u6770\u51fa\u9752\u5e74\u201d\u57fa\u91d1\u3001\u4e2d\u592e\u9ad8\u6821\u57fa\u672c\u4e1a\u52a1\u8d39\u7b49\u7ecf\u8d39\u8d44\u52a9\u3002<\/p>\n<p><strong>\u4e03\u3001\u3010\u56e2\u961f\u5728\u8be5\u9886\u57df\u7684\u5de5\u4f5c\u6c47\u603b\u3011<\/strong><\/p>\n<p>\u672c\u8bfe\u9898\u7ec4\u5728\u53d1\u5c55\u8fc7\u7a0b\u4e2d\u9010\u6e10\u5f62\u6210\u56db\u4e2a\u4e3b\u8981\u7684\u7814\u7a76\u65b9\u5411\uff0c\u5305\u62ec\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u3001\u5355\u5206\u5b50\u5316\u5b66\u3001\u751f\u7269\u5927\u5206\u5b50\u7ed3\u6784\u89e3\u6790\u548c\u7eb3\u7c73\u5b54\u6210\u50cf\u3002<\/p>\n<p>\u5728\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u65b9\u5411\uff0c\u672c\u8bfe\u9898\u7ec4\u63d0\u51fa\u4e86\u4e00\u79cd\u65b0\u578b\u4e14\u901a\u7528\u7684\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u65b9\u6cd5\u5373\u9519\u4f4d\u6d4b\u5e8f\u65b9\u6cd5\uff08Nanopore-Induced Phase-Shift Sequencing\uff0cNIPSS\uff09\u3002\u5229\u7528\u751f\u7269\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u4f53\u7cfb\u4e2d\u7684\u9519\u4f4d\u6548\u5e94\uff0c\u9996\u6b21\u5b9e\u73b0\u4e86\u9488\u5bf9\u975e\u5929\u7136\u6838\u9178FANA\uff08<em>Chem. Sci.<\/em>, 2019, 10, 3110\uff09\uff0cmiRNA\uff08<em>iScience<\/em>, 2020, 23, 100916\uff09\u548c\u591a\u80bd\u7684\u76f4\u63a5\u6d4b\u5e8f\uff08<em>Nano Lett.<\/em>, 2021, 21, 6703\u20136710\uff09\u3002\u5176\u4e2d, \u591a\u80bd\u7684\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u65b9\u6cd5\u4ea6\u83b7\u5f97\u4e86\u300a\u79d1\u6280\u65e5\u62a5\u300b\u7684\u4e13\u8bbf\u62a5\u9053\uff0c\u5728\u56fd\u5185\u5916\u83b7\u5f97\u4e86\u5e7f\u6cdb\u7684\u5173\u6ce8\u3002<\/p>\n<p>\u9488\u5bf9\u5927\u5206\u5b50\u7684\u7ed3\u6784\u89e3\u6790\uff0c\u672c\u8bfe\u9898\u7ec4\u5de7\u5999\u5229\u7528MspA\u7eb3\u7c73\u5b54\u4e00\u5934\u5927\u4e00\u5934\u5c0f\u7684\u7ed3\u6784\u7279\u70b9\uff0c\u4ee5\u201c\u534a\u963b\u5b54\u201d\u68c0\u6d4b\u7b56\u7565\u56de\u907f\u4e86\u6539\u9020\u6574\u4f53\u7ed3\u6784\u5bbd\u5927\u7684\u751f\u7269\u7eb3\u7c73\u5b54\u7684\u9700\u8981\uff0c\u5bf9RNA\uff08<em>Nat. Commun<\/em>., 2021, 12, 3368\uff09\u548c\u591a\u79cd\u86cb\u767d\u8d28\uff08<em>Angew. Chem. Int. Ed.<\/em>, 2021, 60, 23863\u201323870;\u00a0<em>JACS,<\/em>\u00a02022,144, 757\u2013768\uff09\u5b9e\u73b0\u4e86\u5355\u5206\u5b50\u5206\u6790\u3002<\/p>\n<p>\u5728\u5355\u5206\u5b50\u5316\u5b66\u65b9\u5411\uff0c\u672c\u8bfe\u9898\u7ec4\u9996\u6b21\u901a\u8fc7\u6539\u9020\u5185\u8154\u7ed3\u6784\u66f4\u52a0\u9525\u5f62\u7684MspA\u7eb3\u7c73\u5b54\u5b9e\u73b0\u4e86\u7eb3\u7c73\u5b54\u5355\u5206\u5b50\u5316\u5b66\u68c0\u6d4b\u6027\u8d28\u7684\u5927\u5e45\u4f18\u5316\uff0c\u63d0\u51fa\u4e86\u91c7\u7528MspA\u7eb3\u7c73\u5b54\u5355\u5206\u5b50\u53cd\u5e94\u5668\u7684\u65b0\u65b9\u5411\uff08<em>Nat. Commun.<\/em>, 2019, 10, 5668; <em>Chem. Sci.<\/em>, 2020, 11, 879-887\uff09\u3002\u8fdb\u800c\u91c7\u7528\u6838\u9178\u5de5\u7a0b\u66ff\u4ee3\u86cb\u767d\u8d28\u5de5\u7a0b\uff0c\u63d0\u51fa\u4e86\u4e00\u4e2a\u5168\u65b0\u7684\u7eb3\u7c73\u5b54\u5355\u5206\u5b50\u53cd\u5e94\u5668\u6784\u5efa\u7b56\u7565\uff0c\u5e76\u5c06\u8be5\u6280\u672f\u547d\u540d\u4e3aProgrammable Nano Reactor for Stochastic Sensing\uff08PNRSS\uff09\uff08<em>Nat. Commun.<\/em>, 2021, 12, 5811\uff09\uff0c\u53ef\u4ee5\u5b9e\u73b0\u5b54\u9053\u5185\u4efb\u610f\u5316\u5b66\u53cd\u5e94\u7684\u6784\u5efa\u3002\u901a\u8fc7\u66f4\u6df1\u5ea6\u7684\u86cb\u767d\u8d28\u6539\u9020\uff0c\u672c\u8bfe\u9898\u7ec4\u5b9e\u73b0\u4e86\u4e16\u754c\u9996\u4e2a\u5f02\u8d28MspA\u7684\u6784\u5efa\uff0c\u8fdb\u800c\u5b9e\u73b0\u4e86\u6838\u9178\u8868\u89c2\u9057\u4f20\u5b66\u4fee\u9970\uff08<em>Nat<\/em><em>.<\/em><em>\u00a0Nanotechnol.<\/em><em>\uff0c<\/em>\u00a02022, DOI:10.1038\/s41565-022-01169-2\uff09\u3001\u5355\u7cd6(<em>Angew. Chem. Int. 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