{"id":227807,"date":"2021-09-26T06:30:03","date_gmt":"2021-09-25T22:30:03","guid":{"rendered":"\/\/m.iemloyee.com\/?p=227807"},"modified":"2021-09-25T22:18:29","modified_gmt":"2021-09-25T14:18:29","slug":"%e4%b8%ad%e7%a7%91%e9%99%a2%e5%a4%a9%e6%b4%a5%e5%b7%a5%e4%b8%9a%e7%94%9f%e7%89%a9%e6%8a%80%e6%9c%af%e7%a0%94%e7%a9%b6%e6%89%80science%ef%bc%9a%e4%ba%ba%e5%b7%a5%e5%90%88%e6%88%90%e6%b7%80%e7%b2%89","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=227807","title":{"rendered":"\u4e2d\u79d1\u9662\u5929\u6d25\u5de5\u4e1a\u751f\u7269\u6280\u672f\u7814\u7a76\u6240Science\uff1a\u4eba\u5de5\u5408\u6210\u6dc0\u7c89\u5b9e\u73b0\u96f6\u7a81\u7834"},"content":{"rendered":"<p><strong>\u80cc\u666f\u4ecb\u7ecd<\/strong><\/p>\n<p>\u6dc0\u7c89\u662f\u98df\u54c1\u548c\u52a8\u7269\u9972\u6599\u7684\u4e3b\u8981\u70ed\u80fd\u6210\u5206\uff0c\u4e5f\u662f\u91cd\u8981\u7684\u5de5\u4e1a\u539f\u6599\u3002\u690d\u7269\u4e2d\u6dc0\u7c89\u5408\u6210\u6d89\u53ca\u7ea660\u4e2a\u6b65\u9aa4\u548c\u590d\u6742\u7684\u8c03\u63a7\u8fc7\u7a0b\u3002\u7406\u8bba\u80fd\u91cf\u8f6c\u5316\u6548\u7387\u8f83\u4f4e\uff08\u7ea62%\uff09\u3002\u867d\u7136\u4eba\u4eec\u5728\u63d0\u9ad8\u690d\u7269\u6dc0\u7c89\u7684\u4ea7\u91cf\u65b9\u9762\u505a\u4e86\u8bb8\u591a\u52aa\u529b\uff0c\u4f46\u5149\u5408\u4f5c\u7528\u7684\u4f4e\u6548\u6027\u548c\u6dc0\u7c89\u751f\u7269\u5408\u6210\u7684\u590d\u6742\u6027\u6210\u4e3a\u4e3b\u8981\u7684\u969c\u788d\u3002\u76f8\u6bd4\u4e4b\u4e0b\uff0c\u5408\u6210\u751f\u7269\u5b66\u7684\u8fdb\u6b65\u4f7f\u5f97\u5927\u91cf\u5408\u6210\u6dc0\u7c89\u6210\u4e3a\u53ef\u80fd\u3002<\/p>\n<p><strong>\u6210\u679c\u7b80\u4ecb<\/strong><\/p>\n<p><strong>\u8fd1\u65e5\uff0c\u4e2d\u79d1\u9662\u5929\u6d25\u5de5\u4e1a\u751f\u7269\u6280\u672f\u7814\u7a76\u6240\u9a6c\u5ef6\u548c\u56e2\u961f<\/strong>\u4ee5\u4e8c\u6c27\u5316\u78b3\u548c\u6c22\u4f5c\u4e3a\u539f\u6599\uff0c\u91c7\u7528\u4e00\u79cd\u7c7b\u4f3c\u201c\u642d\u79ef\u6728\u201d\u7684\u539f\u7406\uff0c\u901a\u8fc7\u8026\u5408\u3001\u5316\u5b66\u50ac\u5316\u548c\u751f\u7269\u50ac\u5316\u6a21\u5757\u4f53\u7cfb\uff0c\u901a\u8fc711\u6b65\u53cd\u5e94\u5b9e\u73b0\u4e86\u4e8c\u6c27\u5316\u78b3\u5230\u6dc0\u7c89\u7684\u8f6c\u5316\u3002\u5728\u5316\u5b66\u9176\u7cfb\u7edf\u4e2d\uff0c\u4eba\u5de5\u6dc0\u7c89\u5408\u6210\u9014\u5f84\uff08ASAP\uff09\u5728\u6c22\u7684\u9a71\u52a8\u4e0b\uff0c\u4e8c\u6c27\u5316\u78b3\u4f1a\u4ee5\u6bcf\u5206\u949f22 nM\u7684\u901f\u5ea6\u8f6c\u5316\u4e3a\u6dc0\u7c89\uff0c\u6bd4\u7389\u7c73\u4e2d\u5408\u6210\u6dc0\u7c89\u7684\u901f\u5ea6\u9ad8\u7ea68.5\u500d\u3002\u8fd9\u4e00\u9014\u5f84\u4e3a\u4eca\u540e\u5229\u7528CO<sub>2<\/sub>\u5408\u6210\u5316\u5b66-\u751f\u7269\u6742\u5316\u6dc0\u7c89\u5f00\u8f9f\u4e86\u9053\u8def\u3002<strong>\u76f8\u5173\u6210\u679c\u4ee5\u9898\u4e3a<\/strong><strong>\u201cCell-free chemoenzymatic starch synthesis from carbon dioxide\u201d<\/strong><strong>\u53d1\u8868\u5728\u4e86<\/strong><strong>Science<\/strong><strong>\u4e0a\u3002<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-227808\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2021\/09\/1-9.png\" alt=\"\" width=\"600\" height=\"245\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2021\/09\/1-9.png 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2021\/09\/1-9-300x123.png 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p><strong>\u56fe\u6587\u89e3\u6790<\/strong><\/p>\n<p><strong>\u4e00\u3001\u4eba\u5de5\u6dc0\u7c89\u5408\u6210\u9014\u5f84\u7684\u8bbe\u8ba1\u539f\u7406\u4e0e\u6a21\u5757\u5316\u7ec4\u88c5\u3002<\/strong><\/p>\n<p>\u672c\u5de5\u4f5c\u91c7\u7528\u4e00\u79cd\u7c7b\u4f3c\u201c\u642d\u79ef\u6728\u201d\u7684\u65b9\u5f0f\uff0c\u901a\u8fc7\u6a21\u5757\u5316\u7ec4\u88c5\u548c\u66ff\u6362\u7684\u7b56\u7565\uff0c\u5229\u7528\u5316\u5b66\u50ac\u5316\u5242\u5c06\u9ad8\u6d53\u5ea6CO<sub>2<\/sub>\u5728\u9ad8\u5bc6\u5ea6\u6c22\u80fd\u73af\u5883\u4e0b\u8fd8\u539f\u4e3a\u4e00\u78b3\uff08C1\uff09\u5316\u5408\u7269\uff1b\u7136\u540e\u6839\u636e\u5316\u5b66\u805a\u7cd6\u53cd\u5e94\u539f\u7406\u8bbe\u8ba1\u4e86\u78b3\u4e00\u805a\u5408\u65b0\u9176\uff0c\u5c06\u4e00\u78b3\u5316\u5408\u7269\u805a\u5408\u6210\u4e09\u78b3\uff08C3\uff09\u5316\u5408\u7269\uff1b\u6700\u540e\u901a\u8fc7\u4f18\u5316\u751f\u7269\u9014\u5f84\uff0c\u5c06\u4e09\u78b3\u5316\u5408\u7269\u805a\u5408\u6210\u516d\u78b3\uff08C6\uff09\u5316\u5408\u7269\uff0c\u518d\u8fdb\u4e00\u6b65\u5408\u6210\u76f4\u94fe\u548c\u652f\u94fe\u6dc0\u7c89\uff08Cn\u5316\u5408\u7269\uff09\uff0c\u5171\u8ba111\u6b65\u53cd\u5e94\u5b9e\u73b0\u4e86\u4e8c\u6c27\u5316\u78b3\u5230\u6dc0\u7c89\u7684\u8f6c\u5316\u3002\u4e0e\u6b64\u540c\u65f6\uff0c\u672c\u5de5\u4f5c\u8fd8\u901a\u8fc7\u5bf931\u4e2a\u751f\u7269\u4f53\u768462\u79cd\u9176\u768411\u4e2a\u6a21\u5757\u8fdb\u884c\u7ec4\u88c5\u548c\u66ff\u6362\uff0c\u5efa\u7acb\u4e86\u4ee5\u7532\u9187\u4e3a\u8d77\u59cb\u539f\u6599\u768410\u4e2a\u9176\u4fc3\u53cd\u5e94\u7684\u4eba\u5de5\u6dc0\u7c89\u5408\u6210\u9014\u5f84(ASAP) 1.0\u3002\u901a\u8fc7\u540c\u4f4d\u7d20<sup>13<\/sup>C\u6807\u8bb0\u5b9e\u9a8c\u68c0\u6d4bASAP 1.0\u7684\u4e3b\u8981\u4e2d\u95f4\u4f53\u548c\u76ee\u6807\u4ea7\u7269\uff0c\u9a8c\u8bc1\u5176\u5bf9\u7532\u9187\u5408\u6210\u6dc0\u7c89\u7684\u5168\u90e8\u529f\u80fd\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-227809\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2021\/09\/2-9.png\" alt=\"\" width=\"600\" height=\"521\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2021\/09\/2-9.png 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2021\/09\/2-9-300x261.png 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/> <\/p>\n<p>\u56fe1. \u4eba\u5de5\u6dc0\u7c89\u5408\u6210\u9014\u5f84\u7684\u8bbe\u8ba1\u548c\u6a21\u5757\u7ec4\u88c5<\/p>\n<p><strong>\u4e8c\u3001ASAP<\/strong><strong>\u7684\u4e3b\u8981\u74f6\u9888\u3002<\/strong><\/p>\n<p>\u5728\u5efa\u7acbASAP 1.0\u4e4b\u540e\uff0c\u672c\u5de5\u4f5c\u8bd5\u56fe\u901a\u8fc7\u89e3\u51b3\u6f5c\u5728\u7684\u74f6\u9888\u6765\u4f18\u5316\u8be5\u9014\u5f84\u3002\u9996\u5148\uff0c\u7531\u4e8e\u5176\u52a8\u529b\u5b66\u6d3b\u6027\u8f83\u4f4e\uff0c\u5728ASAP 1.0\u4e2d\u9176fls\u5360\u603b\u86cb\u767d\u8d28\u5242\u91cf\u7684\u7ea686%\uff0c\u4ee5\u7ef4\u6301\u4ee3\u8c22\u901a\u91cf\u5e76\u5c06\u6709\u6bd2\u7532\u919b\u4fdd\u6301\u5728\u975e\u5e38\u4f4e\u7684\u6c34\u5e73\u3002\u5b9a\u5411\u8fdb\u5316\u589e\u52a0\u4e86fls\u50ac\u5316\u6d3b\u6027\uff0c\u4ea7\u751f\u4e86\u53d8\u4f53fls-M3\uff0c\u5176\u6d3b\u6027\u63d0\u9ad8\u4e864.7\u500d\uff0c\u4e14\u4ee5\u4e8c\u7f9f\u57fa\u4e19\u916e(DHA)\u4e3a\u4e3b\u3002\u5c3d\u7ba1ATP\u548cADP\u5728\u518d\u751f\u7cfb\u7edf\u7684\u534f\u52a9\u4e0b\u7ef4\u6301\u57281mM\u7684\u4f4e\u6c34\u5e73\uff0c\u4f46ATP\u548cADP\u4ecd\u53ef\u80fd\u90e8\u5206\u6291\u5236\u5927\u80a0\u6746\u83ccfbp\u7684\u529f\u80fd\uff0c\u800c5\u2032-\u5355\u78f7\u9178\u817a\u82f7\u5177\u6709\u4fc3\u6392\u4f5c\u7528\u3002\u4f5c\u8005\u53d1\u73b0\u542b\u6709AMP\u53d8\u6784\u4f4d\u70b92\u4e2a\u7a81\u53d8\u7684\u53d8\u5f02\u4f53fbp-A<sup>R<\/sup>\u7f13\u89e3\u4e86ADP\u6291\u5236\uff0c\u5927\u5e45\u63d0\u9ad8\u4e86DHA\u7684G-6-P\u4ea7\u91cf\u3002\u4e09\u79cd\u6838\u82f7\u9178\u5bf9fbp\u548cfbp-A<sup>R<\/sup>\u7684\u6291\u5236\u6a21\u5f0f\u5206\u6790\u8868\u660eATP\u6216ADP\u662f\u7cfb\u7edf\u6291\u5236\u7684\u51b3\u5b9a\u56e0\u7d20\u3002\u901a\u8fc7\u5c06fbp-A<sup>R<\/sup>\u4e0e\u62a5\u9053\u7684\u5bf9G-6-P\u5177\u6709\u6297\u6027\u7684\u53d8\u4f53\u6574\u5408\uff0c\u7ec4\u5408\u53d8\u4f53fbp-AG<sup>R<\/sup>\u5b9e\u73b0\u4e86\u8fdb\u4e00\u6b65\u7684\u6539\u8fdb\u3002<strong>\u672c\u5de5\u4f5c\u5229\u7528\u8fd9<\/strong><strong>3<\/strong><strong>\u79cd\u5de5\u7a0b\u9176<\/strong><strong>( fls-M3<\/strong><strong>\u3001<\/strong><strong>fbp-AGR<\/strong><strong>\u548c<\/strong><strong>agp-M3 )<\/strong><strong>\u6784\u5efa<\/strong><strong>ASAP 2.0<\/strong><strong>\uff0c\u8be5\u9176\u5728<\/strong><strong>20 m<\/strong><strong>\u7532\u9187\u4e2d<\/strong><strong>10 h<\/strong><strong>\u5185\u4ea7\u751f<\/strong><strong>~ 230 mg L-1<\/strong><strong>\u76f4\u94fe\u6dc0\u7c89\u3002\u4e0e<\/strong><strong>ASAP 1.0<\/strong><strong>\u76f8\u6bd4\uff0c<\/strong><strong>ASAP 2.0<\/strong><strong>\u7684\u6dc0\u7c89\u751f\u4ea7\u7387\u63d0\u9ad8\u4e86<\/strong><strong>7.6<\/strong><strong>\u500d\u3002<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-227810\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2021\/09\/3-7.png\" alt=\"\" width=\"600\" height=\"417\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2021\/09\/3-7.png 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2021\/09\/3-7-300x209.png 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>\u56fe2. ASAP\u4e2d\u7684\u4e3b\u8981\u74f6\u9888\u7684\u89e3\u51b3<\/p>\n<p><strong>\u4e09\u3001\u7531<\/strong><strong>CO<sub>2<\/sub><\/strong><strong>\u7ecf<\/strong><strong>ASAP<\/strong><strong>\u5408\u6210\u6dc0\u7c89\u3002<\/strong><\/p>\n<p>\u7531\u4e8e\u4e8c\u6c27\u5316\u78b3\u52a0\u6c22\u7684\u4e0d\u5229\u6761\u4ef6\uff0c\u672c\u5de5\u4f5c\u5728ASAP 3.0\u4e2d\u5f00\u53d1\u4e86\u5177\u6709\u5316\u5b66\u53cd\u5e94\u5355\u5143\u548c\u9176\u50ac\u5316\u53cd\u5e94\u5355\u5143\u7684\u5316\u5b66\u9176\u7ea7\u8054\u7cfb\u7edf\u3002\u4e3a\u4e86\u6ee1\u8db3fls\u5bf9\u9ad8\u6d53\u5ea6\u7532\u919b\u7684\u9700\u6c42\uff0c\u5e76\u907f\u514d\u7532\u919b\u5bf9\u5176\u4ed6\u9176\u7684\u6bd2\u6027\uff0c\u672c\u5de5\u4f5c\u8fdb\u4e00\u6b65\u5bf9\u9176\u5355\u5143\u8fdb\u884c\u4e86\u4e24\u6b65\u64cd\u4f5c\u3002\u5728\u5316\u5b66\u53cd\u5e94\u5355\u5143\u4e2d\uff0cCO<sub>2<\/sub>\u4ee5~ 0.25 g h<sup>-1<\/sup>g<sup>-1<\/sup>\u50ac\u5316\u5242\u7684\u901f\u7387\u5316\u5b66\u52a0\u6c22\u751f\u6210\u7532\u9187\uff0c\u751f\u6210\u7684\u7532\u9187\u5728\u7b2c\u4e00\u4e2a\u5c0f\u65f6\u5185\u4e0d\u65ad\u51b7\u51dd\u5e76\u901a\u5165\u9176\u5355\u5143\u6700\u7ec8\u6d53\u5ea6~ 100 mM\u3002\u5728\u9176\u5355\u5143\u4e2d\uff0c\u5148\u8865\u51452\u79cd\u6838\u5fc3\u9176\u548c\u8f85\u52a9\u8fc7\u6c27\u5316\u6c22\u9176(cat)\uff0c\u4f7f\u7532\u9187\u518d\u8f6c\u5316\u4e3a~ 22.5 mM C3\u4e2d\u95f4DHA\uff0c\u518d\u8865\u5145\u5176\u4f598\u79cd\u6838\u5fc3\u9176\u548c\u8f85\u52a9\u7ec4\u5206\uff0c\u5728\u968f\u540e2 h\u8f6c\u5316\u4e3a~1.6\u514b\u5347\u76f4\u94fe\u6dc0\u7c89\u3002\u5728\u7898\u6eb6\u6db2\u5b58\u5728\u4e0b\uff0c\u5408\u6210\u7684\u76f4\u94fe\u6dc0\u7c89\u5177\u6709\u4e0e\u6807\u51c6\u76f4\u94fe\u6dc0\u7c89\u76f8\u540c\u7684\u6df1\u84dd\u8272\u989c\u8272\u548c\u6700\u5927\u5438\u6536\u503c\u3002\u5408\u6210\u7684\u652f\u94fe\u6dc0\u7c89\u5448\u7ea2\u68d5\u8272\uff0c\u7898\u5904\u7406\u540e\u7684\u5438\u6536\u5cf0\u4e0e\u6807\u51c6\u652f\u94fe\u6dc0\u7c89\u76f8\u5f53\u3002\u5408\u6210\u7684\u76f4\u94fe\u6dc0\u7c89\u548c\u652f\u94fe\u6dc0\u7c89\u7684\u8868\u73b0\u51fa\u4e0e\u6807\u51c6\u6837\u54c1\u76f8\u540c\u76841~6\u4e2a\u8d28\u5b50\u6838\u78c1\u5171\u632f\u4fe1\u53f7\u3002\u901a\u8fc7\u7a7a\u95f4\u548c\u65f6\u95f4\u5206\u6b65\u5206\u79bb\uff0cASAP 3.0\u5728CO<sub>2<\/sub>\u6d53\u5ea6\u4e3a410 mg L<sup>-1<\/sup>h<sup>-1<\/sup>\u7684\u6761\u4ef6\u4e0b\u83b7\u5f97\u4e86\u8f83\u9ad8\u7684\u6dc0\u7c89\u4ea7\u7387\u3002\u8be5\u5316\u5b66\u9176\u9014\u5f84\u7684\u6dc0\u7c89\u5408\u6210\u901f\u7387\u8fbe\u5230\u603b\u50ac\u5316\u5242\u548c\u86cb\u767d\u8d28\u768422 nmol min<sup>-1<\/sup>mg<sup>-1<\/sup>\uff0c\u6bd4\u7389\u7c73\u4e2d\u5408\u6210\u6dc0\u7c89\u7684\u901f\u5ea6\u9ad8\u7ea68.5\u500d\u3002ASAP \u4ee5\u65e0\u7ec6\u80de\u3001\u5316\u5b66\u9176\u4fc3\u548c\u9ad8\u6548\u7684\u65b9\u5f0f\u4eceCO<sub>2<\/sub>\u5408\u6210\u6dc0\u7c89\uff0c\u4e3a\u6dc0\u7c89\u7684\u5de5\u4e1a\u751f\u7269\u5236\u9020\u7b49\u5e94\u7528\u63d0\u4f9b\u4e86\u91cd\u8981\u7684\u8d77\u70b9\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-227811\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2021\/09\/4-6.png\" alt=\"\" width=\"600\" height=\"430\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2021\/09\/4-6.png 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2021\/09\/4-6-300x215.png 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>\u56fe3. 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