{"id":221492,"date":"2021-05-10T06:19:44","date_gmt":"2021-05-09T22:19:44","guid":{"rendered":"\/\/m.iemloyee.com\/?p=221492"},"modified":"2021-05-09T19:20:12","modified_gmt":"2021-05-09T11:20:12","slug":"%e6%84%8f%e5%a4%a7%e5%88%a9%e7%90%86%e5%b7%a5%e5%ad%a6%e9%99%a2%e5%a4%a7%e5%ad%a6%e5%8d%a1%e8%80%90%e5%9f%ba%e6%a2%85%e9%9a%86%e5%a4%a7%e5%ad%a6sci-adv-%e7%9f%b3%e5%a2%a8%e7%83%af%e5%be%ae%e7%94%b5","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=221492","title":{"rendered":"\u610f\u5927\u5229\u7406\u5de5\u5b66\u9662\u5927\u5b66&#038;\u5361\u8010\u57fa\u6885\u9686\u5927\u5b66Sci. Adv.:\u77f3\u58a8\u70ef\u5fae\u7535\u6781\u76d1\u6d4b\u5fc3\u808c\u7ec6\u80de\u5185\u52a8\u4f5c\u7535\u4f4d"},"content":{"rendered":"<p>\u3010<strong><b>\u5f15\u8a00<\/b><\/strong>\u3011<\/p>\n<p>\u76d1\u6d4b\u795e\u7ecf\u5143\u548c\u5fc3\u808c\u7ec6\u80de\u7684\u7535\u6d3b\u52a8\u5bf9\u4e8e\u7814\u7a76\u5927\u8111\u548c\u5fc3\u810f\u7684\u7535\u751f\u7406\uff0c\u7814\u7a76\u795e\u7ecf\u9000\u884c\u6027\u75be\u75c5\u6216\u5fc3\u810f\u75be\u75c5\u4ee5\u53ca\u5236\u5b9a\u65b0\u7684\u6cbb\u7597\u7b56\u7565\u81f3\u5173\u91cd\u8981\u3002\u5fae\u7535\u6781\u9635\u5217\uff08MEA\uff09\u5e73\u53f0\u5df2\u88ab\u5e7f\u6cdb\u4f7f\u7528\uff0c\u56e0\u4e3a\u5b83\u4eec\u80fd\u591f\u901a\u8fc7\u540c\u65f6\u6d4b\u91cf\u6765\u81ea\u6210\u767e\u4e0a\u5343\u4e2a\u7ec6\u80de\u7684\u7535\u4fe1\u53f7\u6765\u76d1\u89c6\u5927\u578b\u7ec6\u80de\u96c6\u5408\u4f53\u3002\u5728\u4f53\u5916\u5e94\u7528\u4e2d\uff0c\u7531\u4e8e\u6700\u8fd1\u5b9e\u73b0\u4e86\u65b0\u9896\u7684\u4e8c\u7ef4\uff082D\uff09\u548c3D\u91d1\u5c5e\u7535\u6781\u914d\u7f6e\uff0cMEA\u65b9\u6cd5\u5728\u51c6\u786e\u5ea6\u548c\u9ad8\u901a\u91cf\u4e4b\u95f4\u5b9e\u73b0\u4e86\u7406\u60f3\u7684\u5e73\u8861\uff0c\u4ece\u800c\u53ef\u4ee5\u8bb0\u5f55\u7c7b\u7ec6\u80de\u5185\u52a8\u4f5c\u7535\u4f4d\uff08APs\uff09\u3002\u77f3\u58a8\u70ef\u7684\u975e\u51e1\u6027\u80fd\uff0c\u5982\u751f\u7269\u76f8\u5bb9\u6027\uff0c\u9ad8\u7535\u5bfc\u7387\u548c\u67d4\u97e7\u6027\uff0c\u4f7f\u5176\u6210\u4e3a\u5f00\u53d1MEA\u548c\u6676\u4f53\u7ba1\u7684\u7406\u60f3\u9009\u62e9\uff0c\u7528\u4ee5\u7814\u7a76\u53ef\u5174\u594b\u7ec6\u80de\u7684\u7535\u751f\u7406\u5b66\u3002\u4f46\u662f\uff0c\u8fc4\u4eca\u4e3a\u6b62\uff0c\u57fa\u4e8e\u77f3\u58a8\u70ef\u7684\u5668\u4ef6\u4ec5\u9650\u4e8e\u7ec6\u80de\u5916\u573a\u7535\u52bf\uff08FP\uff09\u8bb0\u5f55\uff0c\u8be5\u8bb0\u5f55\u4e0d\u80fd\u50cf\u4f7f\u7528\u819c\u7247\u94b3\u90a3\u6837\u6355\u83b7AP\u7684\u4e3b\u8981\u7279\u5f81\u3002\u8fd9\u6781\u5927\u5730\u9650\u5236\u4e86\u77f3\u58a8\u70ef\u5fae\u7535\u6781\u5728\u4f53\u5916\u7535\u751f\u7406\u4e2d\u7684\u9002\u7528\u6027\uff0c\u56e0\u4e3a\u51c6\u786e\u5730\u76d1\u6d4b\u7ec6\u80de\u5185AP\u7684\u80fd\u529b\u662f\u6bd2\u7406\u5b66\u7814\u7a76\u548c\u836f\u7269\u7b5b\u9009\u6d4b\u5b9a\u7684\u57fa\u672c\u8981\u6c42\u3002<\/p>\n<p>\u3010<strong><b>\u6210\u679c\u7b80\u4ecb<\/b><\/strong>\u3011<\/p>\n<p>\u77f3\u58a8\u70ef\u5177\u6709\u72ec\u7279\u7684\u7535\u6027\u80fd\uff0c\u662f\u78b3\u57fa\u751f\u7269\u4f20\u611f\u5668\uff08\u5982\u5fae\u7535\u6781\u548c\u573a\u6548\u5e94\u6676\u4f53\u7ba1\uff09\u7684\u5019\u9009\u6750\u6599\u3002\u8fd1\u5e74\u6765\uff0c\u77f3\u58a8\u70ef\u751f\u7269\u4f20\u611f\u5668\u5df2\u6210\u529f\u7528\u4e8e\u5728\u53ef\u5174\u594b\u7ec6\u80de\u4e2d\u8fdb\u884c\u52a8\u4f5c\u7535\u4f4d\u7684\u7ec6\u80de\u5916\u8bb0\u5f55\u3002\u7136\u800c\uff0c\u7531\u4e8e\u7f3a\u4e4f\u6709\u6548\u7684\u7ec6\u80de\u7a7f\u5b54\u65b9\u6cd5\uff0c\u7ec6\u80de\u5185\u8bb0\u5f55\u4ecd\u7136\u8d85\u51fa\u4e86\u5b83\u4eec\u76ee\u524d\u7684\u80fd\u529b\u3002<strong><b>\u610f\u5927\u5229\u7406\u5de5\u5b66\u9662\u5927\u5b66&amp;\u5361\u8010\u57fa\u6885\u9686\u5927\u5b66<\/b><\/strong>\u63d0\u51fa\u4e86\u4e00\u79cd\u7531\u5e73\u9762\u5916\u751f\u957f\u7684\u4e09\u7ef4\u6a21\u7cca\u77f3\u58a8\u70ef\uff083DFG\uff09\u7ec4\u6210\u7684\u5fae\u7535\u6781\u5e73\u53f0\uff0c\u8be5\u5e73\u53f0\u80fd\u591f\u8bb0\u5f55\u5177\u6709\u9ad8\u4fe1\u566a\u6bd4\u7684\u7ec6\u80de\u5185\u5fc3\u810f\u52a8\u4f5c\u7535\u4f4d\u3002\u4f5c\u8005\u5229\u7528\u8d85\u5feb\u8109\u51b2\u6fc0\u5149\u901a\u8fc7\u70ed\u8f7d\u6d41\u5b50\u7684\u4ea7\u751f\u6765\u7a7f\u5b54\u7ec6\u80de\u819c\uff0c\u5e76\u57283DFG\u7535\u6781\u548c\u7ec6\u80de\u5185\u533a\u57df\u4e4b\u95f4\u5efa\u7acb\u7d27\u5bc6\u7684\u63a5\u89e6\u3002\u8fd9\u79cd\u65b9\u6cd5\u4f7f\u80fd\u591f\u68c0\u6d4b\u836f\u7269\u5bf9\u4eba\u6e90\u6027\u5fc3\u808c\u7ec6\u80de\u52a8\u4f5c\u7535\u4f4d\u5f62\u72b6\u7684\u5f71\u54cd\u3002\u7ed3\u5408\u6fc0\u5149\u7a7f\u5b54\u76843DFG\u7535\u6781\u53ef\u7528\u4e8e\u5168\u78b3\u7ec6\u80de\u5185\u5fae\u7535\u6781\u9635\u5217\uff0c\u4ee5\u76d1\u6d4b\u7ec6\u80de\u7684\u7535\u751f\u7406\u72b6\u6001\u3002\u8be5\u6210\u679c\u4ee5\u9898\u4e3a<strong>\u201c<\/strong><strong>Intracellular action potential recordings from cardiomyocytes by ultrafast pulsed laser irradiation of fuzzy graphene microelectrodes<\/strong><strong>\u201d<\/strong>\u53d1\u8868\u5728<strong><b>Sci. Adv.<\/b><\/strong><strong>\u4e0a<\/strong>\u3002<\/p>\n<p><strong><b>\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/b><\/strong><\/p>\n<p><strong><b>\u56fe<\/b><\/strong><strong>1<\/strong><strong>.<\/strong><strong>3DFG SEM\u6210\u50cf\u548c\u5149\u5b66\u8868\u5f81<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-221494\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2021\/05\/r1-1.jpg\" alt=\"\" width=\"600\" height=\"388\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2021\/05\/r1-1.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2021\/05\/r1-1-300x194.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>\uff08A\uff095-\u03bcm\u00a03DFG\u7535\u6781\u7684SEM\u56fe\u50cf\u00a0\uff08B\uff09\u7194\u878d\u77f3\u82f1\uff08\u7070\u8272\uff09\uff0c\u5728800\u00b0C\u5408\u621010\u5206\u949f\uff08\u7ea2\u8272\uff09\u76843DFG\u548c\u5728800\u00a0\u00b0C\u5408\u621030\u5206\u949f\uff08\u84dd\u8272\uff09\u76843DFG\u7684UV-vis\u5438\u5149\u5ea6\u4e0e\u6ce2\u957f\u7684\u5173\u7cfb \uff08C\uff09\u5728\u53ef\u89c1\u5149\u548c\u8fd1\u7ea2\u5916\u8303\u56f4\u51853DFG\u4ecb\u7535\u5e38\u6570\u7684\u5b9e\u90e8\u548c\u865a\u90e8 \uff08D\uff09\u57281064 nm\u7684\u8d85\u5feb\uff08\u76ae\u79d2\uff09\u8109\u51b2\u6fc0\u5149\u6fc0\u53d1\u4e0b\uff0c\u57283DFG\u7535\u6781\u548cPBS\u4e4b\u95f4\u7684\u754c\u9762\u4e0a\u4ee5\u4e0d\u540c\u7684\u6fc0\u5149\u5f3a\u5ea6\u4ea7\u751f\u7684\u5149\u7535\u6d41 \uff08E\uff09\u6fc0\u5149\u6fc0\u53d1\u4ea7\u751f\u7684\u5149\u7535\u6d41\u7684\u7535\u5bb9\u548c\u6cd5\u62c9\u7b2c\u7535\u6d41\u5206\u91cf<\/p>\n<p><strong><b>\u56fe<\/b><\/strong><strong>2.3DFG ME<\/strong><strong>A-<\/strong><strong>\u5fc3\u808c\u7ec6\u80de\u754c\u9762<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-221495\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2021\/05\/r2-1.jpg\" alt=\"\" width=\"600\" height=\"273\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2021\/05\/r2-1.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2021\/05\/r2-1-300x137.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>\uff08A\uff09\u57283DFG-MEA\u4e0a\u4f53\u5916\u57f9\u517b7\u5929\u7684hiPSC-CM\u7684\u660e\u573a\uff08I\u548cIII\uff09\u548c\u514d\u75ab\u8367\u5149\u56fe\u50cf\uff08II\u548cIV\uff09\uff08B\uff09\u57283DFG MEA\u4e0a\u7684hiPSC-CM\u7684\u4f2a\u5f69\u8272SEM\u56fe\u50cf \uff08C\uff093DFG\u4e0aHL-1\u7ec6\u80de\u7684\u6a2a\u622a\u9762SEM\u56fe\u50cf\u3002 \u53f3\u56fe\u663e\u793a\u7eff\u8272\u7684\u7ec6\u80de\u6838\uff0c\u84dd\u8272\u7684\u7ec6\u80de\u8d28\u548c\u7ea2\u8272\u76843DFG<\/p>\n<p><strong>\u56fe<\/strong><strong><b>3<\/b><\/strong><strong>.<\/strong><strong>\u7535\u751f\u7406\u8bb0\u5f55<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-221496\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2021\/05\/r3-1.jpg\" alt=\"\" width=\"600\" height=\"511\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2021\/05\/r3-1.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2021\/05\/r3-1-300x256.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>\uff08A\uff09\u4f7f\u75283DFG-MEA\u548c50-\u03bcm\u7535\u6781\uff08n = 80\u4e2a\u7535\u6781\uff09\u5bf9hiPSC-CM\u8fdb\u884c\u4ee3\u8868\u6027\u7684\u7ec6\u80de\u5916FP\u8bb0\u5f55\uff08B\uff09\u6e17\u900f\u540e\u5728\u5177\u670950\u03bcm\u7535\u6781\u76843DFG-MEA\u4e0a\u8bb0\u5f55\u4ee3\u8868\u6027\u7ec6\u80de\u5185AP\uff08n = 70\u4e2a\u7535\u6781\uff09\uff08C\uff09\u7ec6\u80de\u819c\u91cd\u5851\u548c\u7ec6\u80de\u5916FP\u53e0\u52a0\u540e\u7ec6\u80de\u5185\u5076\u8054\u7684\u65f6\u95f4\u7a33\u5b9a\u6027<\/p>\n<p><strong><b>\u56fe<\/b><\/strong><strong>4.<\/strong><strong>\u5bf9\u5fc3\u808c\u7ec6\u80de\u7684\u590d\u5408\u4f5c\u7528<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-221497\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2021\/05\/r4-1.jpg\" alt=\"\" width=\"600\" height=\"721\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2021\/05\/r4-1.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2021\/05\/r4-1-250x300.jpg 250w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>\uff08A\uff09\u65bd\u75282\u03bcME-4031\u540e\u7684\u4ee3\u8868\u6027\u5fc3\u810fFP\u548cAP\uff08B\uff09\u5404\u79cd\u6d53\u5ea6\u7684\u785d\u82ef\u5730\u5e73\u7ed9\u836f\u524d\u540e\u7684\u4ee3\u8868\u6027\u5fc3\u810fAP \uff08C\uff09\u65bd\u7528100 nM\u591a\u82ac\u5229\u7279\uff08DOF\uff09\u524d\u540e\u7684\u4ee3\u8868\u6027\u5fc3\u810fAP<\/p>\n<p><strong><b>\u3010<\/b><\/strong><strong><b>\u5c0f\u7ed3<\/b><\/strong><strong><b>\u3011<\/b><\/strong><\/p>\n<p>\u5728\u8fd9\u9879\u5de5\u4f5c\u4e2d\uff0c\u4f5c\u8005\u4ecb\u7ecd\u4e86\u57fa\u4e8e3DFG\u7684MEA\u5e73\u53f0\uff0c\u8be5\u5e73\u53f0\u80fd\u591f\u8bb0\u5f55\u4eba\u6e90\u6027\u5fc3\u808c\u7ec6\u80de\u7684\u7ec6\u80de\u5185\u7535\u6d3b\u52a8\u3002\u901a\u8fc73DFG\u72ec\u7279\u7684\u5f62\u6001\uff0c\u5149\u5b66\u548c\u7535\u5316\u5b66\u7279\u6027\u7684\u7ec4\u5408\uff0c\u53ef\u4ee5\u8fdb\u884cAP\u8bb0\u5f55\u30023DFG\u5728\u8d85\u5feb\u8109\u51b2\u6fc0\u5149\u7167\u5c04\u4e0b\u5bf9\u7ec6\u80de\u819c\u7684\u62ee\u6297\u4f5c\u7528\u5f52\u56e0\u4e8e\u6750\u6599\u4e2d\u70ed\u8f7d\u6d41\u5b50\u7684\u4ea7\u751f\u30023DFG MEA\u5728\u6d4b\u91cf\u5fc3\u808c\u7ec6\u80de\u57f9\u517b\u7269\u4e2d\u7684\u79bb\u5b50\u7535\u6d41\u65b9\u9762\u663e\u793a\u51fa\u6700\u4f73\u6027\u80fd\uff0c\u53ef\u4e3a\u7ec6\u80de\u5185AP\u63d0\u4f9b\u9ad8SNR\uff0c\u5e76\u5141\u8bb8\u51c6\u786e\u68c0\u6d4b\u836f\u7269\u5bf9\u5fc3\u810f\u79bb\u5b50\u901a\u9053\u7684\u5f71\u54cd\u30023DFG\u4e0a\u7684\u7ec6\u80de\u7a7f\u5b54\u8fc7\u7a0b\u663e\u793a\u51fa\u6781\u4f4e\u7684\u4fb5\u88ad\u6027\uff0c\u56e0\u4e3a\u5b83\u53ef\u4ee5\u5728\u540c\u4e00\u7ec6\u80de\u4e0a\u91cd\u590d\u8fdb\u884c\u800c\u4e0d\u4f1a\u5f71\u54cd\u5176\u5065\u5eb7\u6216\u81ea\u53d1\u7684\u7535\u6d3b\u52a8\u3002\u4f7f\u7528\u77f3\u58a8\u70ef\u7535\u6781\u8fdb\u884c\u7ec6\u80de\u5185AP\u8bb0\u5f55\u7684\u53ef\u80fd\u6027\u4e3a\u9ad8\u6027\u80fd\uff0c\u5168\u78b3MEA\u751f\u7269\u4f20\u611f\u5668\u4ef6\u7684\u5b9e\u73b0\u94fa\u5e73\u4e86\u9053\u8def\u3002\u8fd9\u4e9b\u751f\u7269\u7535\u5b50\u5b66\u53ef\u4ee5\u63d0\u4f9b\u4e00\u4e9b\u57fa\u672c\u529f\u80fd\uff0c\u4f8b\u5982\u7528\u4e8e\u4f53\u5185\u5e94\u7528\u7684\u9ad8\u673a\u68b0\u7075\u6d3b\u6027\uff0c\u7528\u4e8e\u957f\u671f\u5b9e\u9a8c\u7684\u9ad8\u751f\u7269\u76f8\u5bb9\u6027\uff0c\u7528\u4e8e\u836f\u7269\u7b5b\u9009\u5e94\u7528\u7684\u4f4e\u6210\u672c\u4ee5\u53ca\u51fa\u4e8e\u73af\u5883\u8003\u8651\u800c\u6ca1\u6709\u8d35\u91d1\u5c5e\u6216\u6c61\u67d3\u6750\u6599\u7684\u60c5\u51b5\u3002\u672a\u6765\u7684\u5de5\u4f5c\u5c06\u5305\u62ec\u901a\u8fc7\u5bfc\u7ebf\u6216\u7ba1\u7684\u6a21\u677f\u751f\u957f\u6216\u8868\u9762\u5316\u5b66\u4fee\u9970\u6765\u7a33\u5b9a\u7ec6\u80de\u5185\u7684\u8bb0\u5f55\u3002<\/p>\n<p><strong><b>\u6587\u732e\u94fe\u63a5<\/b><\/strong>\uff1aIntracellular action potential recordings from cardiomyocytes by ultrafast pulsed laser irradiation of fuzzy graphene microelectrodes. Sci. 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