{"id":218483,"date":"2021-03-02T06:07:54","date_gmt":"2021-03-01T22:07:54","guid":{"rendered":"\/\/m.iemloyee.com\/?p=218483"},"modified":"2021-03-01T21:08:04","modified_gmt":"2021-03-01T13:08:04","slug":"%e4%b8%ad%e5%9b%bd%e7%a7%91%e5%ad%a6%e6%8a%80%e6%9c%af%e5%a4%a7%e5%ad%a6%e9%82%93%e5%85%86%e7%a5%a5-j-am-chem-soc-%e9%97%aa%e9%80%9f%e5%90%88%e6%88%90dna%e5%af%86%e5%ba%a6%e6%9c%80%e9%ab%98","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=218483","title":{"rendered":"\u4e2d\u56fd\u79d1\u5b66\u6280\u672f\u5927\u5b66\u9093\u5146\u7965 J. Am. Chem. Soc.:\u95ea\u901f\u5408\u6210DNA\u5bc6\u5ea6\u6700\u9ad8\u7684\u7403\u5f62\u6838\u9178"},"content":{"rendered":"

\u3010\u5f15\u8a00<\/b><\/strong>\u3011<\/p>\n

\u7403\u5f62\u6838\u9178\uff08SNA\uff09\u5373DNA\u591a\u529f\u80fd\u5316\u7eb3\u7c73\u9897\u7c92\uff08NP\uff09\uff0c\u662fDNA\u76f8\u5173\u7ec4\u88c5\u3001\u4f20\u611f\u548c\u6cbb\u7597\u7b49\u5e94\u7528\u7684\u5173\u952e\u7eb3\u7c73\u5355\u5143\u3002SNA\u5177\u6709\u7d27\u5bc6\u5ac1\u63a5\u7684DNA\u94fe\u548c\u7531\u6b64\u5e26\u6765\u7684\u8bf8\u591a\u72ec\u7279\u6027\u8d28\uff0c\u5305\u62ec\u53ef\u8c03\u7684\u6742\u4ea4\u6d3b\u6027\u3001\u589e\u5f3a\u7684\/\u591a\u4ef7\u9776\u6807\u7ed3\u5408\u3001\u9661\u5ced\u7684\u7194\u89e3\u8f6c\u53d8\u66f2\u7ebf\u3001\u6297\u6838\u9178\u9176\/\u76d0\u7a33\u5b9a\u6027\u548c\u65e0\u8f7d\u4f53\u7ec6\u80de\u6444\u53d6\u7b49\u3002SNA\u5408\u6210\u901a\u5e38\u59cb\u4e8e\u6c34\u6eb6\u6db2\u4e2d\u5e26\u6709\u5def\u57fa\u672b\u7aef\u7684DNA\u4e0e\u91d1\u7eb3\u7c73\u9897\u7c92\uff08AuNP\uff09\u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u3002DNA\u548cAuNP\u4e0a\u7684\u8d1f\u7535\u8377\u4f1a\u5bfc\u81f4\u5f3a\u70c8\u7684\u5e93\u4ed1\u6392\u65a5\uff0c\u4e0d\u5229\u4e8eDNA\u63a5\u8fd1\u91d1\u8868\u9762\u3002\u4e3a\u4e86\u514b\u670d\u6b64\u969c\u788d\uff0c\u9700\u8981\u6dfb\u52a0\u76d0\u4ee5\u5c4f\u853d\u9759\u7535\u529b\uff0c\u4f46\u8fd9\u4f1a\u589e\u52a0NP\u56e2\u805a\u7684\u98ce\u9669\u3002Mirkin\u7b49\u4eba\u610f\u8bc6\u5230\u8fd9\u4e00\u70b9\uff0c\u91c7\u7528\u9010\u6b65\u52a0\u76d0\u548c\u5ef6\u957f\u9648\u5316\u65f6\u95f4\u4ee5\u6700\u5927\u5316DNA\u8d1f\u8f7d\u91cf\u3002\u540c\u6837\uff0c\u8868\u9762\u6d3b\u6027\u5242\u4e5f\u53ef\u7528\u4e8e\u7a33\u5b9aAuNP\u3002<\/p>\n

\u3010\u6210\u679c\u7b80\u4ecb<\/b><\/strong>\u3011<\/p>\n

\u4fee\u9970\u6709\u9ad8\u5bc6\u5ea6DNA\u94fe\u7684\u7eb3\u7c73\u7c92\u5b50\uff08NP\uff09\uff0c\u4e5f\u79f0\u4e3a\u7403\u5f62\u6838\u9178\uff08SNA\uff09\uff0c\u5e7f\u6cdb\u7528\u4e8e\u53ef\u7f16\u7a0b\u7684DNA\u7ec4\u88c5\u3001\u4f20\u611f\u3001\u6210\u50cf\u548c\u6cbb\u7597\u3002\u5e38\u89c4\u7684SNA\u5408\u6210\u8fc7\u7a0b\u975e\u5e38\u8017\u65f6\uff0c\u4e14\u9700\u8981\u683c\u5916\u5c0f\u5fc3\u4ee5\u907f\u514dNP\u805a\u96c6\u3002\u4e2d\u56fd\u79d1\u5b66\u6280\u672f\u5927\u5b66\u9093\u5146\u7965\u7b49\u4eba<\/b><\/strong>\u62a5\u9053\u4e86\u4e00\u79cd\u6781\u5176\u7b80\u5355\u3001\u5feb\u901f\u3001\u9ad8\u6548\u4e14\u53ef\u671b\u89c4\u6a21\u5316\u5236\u5907\u7684\u8fc7\u7a0b\uff0c\u4f5c\u8005\u79f0\u4e4b\u4e3a\u201cINDEBT\u201d(Instantaneous Dehydration in Butanol)\uff0c\u53ef\u77ac\u95f4\u751f\u6210\u5177\u6709\u521b\u7eaa\u5f55\u7684\u9ad8DNA\u5bc6\u5ea6SNA\u3002\u8be5\u65b9\u6cd5\u4f9d\u8d56\u4e8eDNA\/NP\u6df7\u5408\u7269\u548c\u4e01\u9187\u76f8\u63a5\u89e6\u65f6\u7684\u5feb\u901f\u9664\u6c34\u3002\u8be5\u8fc7\u7a0b\u4ea7\u751f\u5747\u5300\u6df7\u5408\u7684DNA\/NP\u8131\u6c34\u201c\u56fa\u6eb6\u4f53\u201d\uff0c\u5927\u5927\u52a0\u901f\u4e86DNA\u5728NP\u8868\u9762\u7684Au-S\u952e\u951a\u5b9a\u53cd\u5e94\u3002\u4e0e\u6587\u732e\u4e2d\u6700\u5148\u8fdb\u7684DNA\u4fee\u9970\u7b56\u7565\u76f8\u6bd4\uff0c\u4e01\u9187\u4e2d\u77ac\u95f4\u8131\u6c34\uff08INDEBT\uff09\u6700\u9ad8\u80fd\u4f7fDNA\u5bc6\u5ea6\u589e\u52a0\u81f33\u500d\u5de6\u53f3\u3002\u8fd9\u79cd\u8d85\u81f4\u5bc6\u7684DNA\u5ac1\u63a5\u53cd\u5e94\u5728\u51e0\u79d2\u949f\u5185\u5373\u53ef\u5b8c\u6210\uff0c\u6240\u5f97SNA\u53ef\u7528\u4e8e\u9ad8\u5bc6\u5ea6\u6838-\u536b\u661f\u7ec4\u88c5\u4f53\u7684\u6784\u5efa\u3002\u8fd9\u4e00\u5de5\u4f5c\u4f7fSNA\u5408\u6210\u53d8\u5f97\u8f7b\u800c\u6613\u4e3e\uff0c\u5e76\u4f7f\u672a\u6765\u80fd\u591f\u63a2\u7d22\u5177\u6709\u8d85\u9ad8DNA\u5bc6\u5ea6SNA\u7684\u7269\u7406\u3001\u5316\u5b66\u548c\u751f\u7269\u5b66\u6548\u5e94\u3002\u8be5\u6210\u679c\u4ee5\u201c<\/b><\/strong>Flash Synthesis of Spherical Nucleic Acids with Record DNA Density<\/b><\/strong>\u201d<\/b><\/strong>\u4e3a\u9898\u53d1\u8868\u5728J. Am. Chem. Soc.<\/b><\/strong>\u4e0a<\/b><\/strong>\u3002<\/p>\n

\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/b><\/strong><\/p>\n

\u56fe1<\/b><\/strong>. <\/b><\/strong>\u5178\u578b\u7684INDEBT\u8fc7\u7a0b<\/b><\/strong><\/p>\n

\"\"<\/p>\n

\uff08A\uff09\u64cd\u4f5c\u6d41\u7a0b\uff1b\uff08B-C\uff09\u51dd\u80f6\u7535\u6cf3\u7ed3\u679c\u663e\u793a\uff0c\u968f\u4e01\u9187\uff1aH2<\/sub>O\u4f53\u79ef\u6bd4\u589e\u52a0\uff0c\u67e0\u6aac\u9178\u76d0\uff08B\uff09\u548c\u78fa\u5316\u4e09\u82ef\u57fa\u81a6\uff08C\uff09\u4fdd\u62a4\u768415 nm AuNP\u8868\u9762DNA\u4fee\u9970\u91cf\u5148\u7f13\u6162\u589e\u52a0\uff08B\uff09\u6216\u51e0\u4e4e\u4e0d\u53d8\uff08C\uff09\uff0c\u53d1\u751f\u8131\u6c34\uff08INDEBT\uff09\u65f6DNA\u63a5\u679d\u91cf\u6025\u5267\u589e\u52a0\u3002<\/p>\n

\u56fe2.<\/b><\/strong>\u00a0DNA\u63a5\u679d\u91cf\u7684\u6d4b\u5b9a<\/b><\/strong><\/p>\n

\"\"<\/p>\n

\uff08A-D\uff09\u901a\u8fc7\u51b7\u51bb\u548cINDEBT\u83b7\u5f97\u7684\u7cfb\u5217\u5c3a\u5bf8\uff085-45 nm AuNP\uff0c21-89b DNA\uff09SNA\u7684\u7535\u6cf3\u56fe\u548c\u8367\u5149DNA\u5b9a\u91cf\uff1b\uff08E\uff09\u4e0d\u540c\u65b9\u6cd5\u5236\u5907\u7684SNA\u7684DNA\u4fee\u9970\u5bc6\u5ea6\u5bf9\u6bd4\u3002<\/p>\n

\u56fe<\/b><\/strong>3.<\/b><\/strong>\u00a0\u51b7\u51bb-INDEBT\u5206\u6b65\u4fee\u9970\u53ca\u7eb3\u7c73\u8367\u5149\u63a2\u9488\u5236\u5907<\/b><\/strong><\/p>\n

\"\"<\/p>\n

\uff08A\uff09\u901a\u8fc7\u51b7\u51bb-INDEBT\u5206\u6b65\u8fc7\u7a0b\u83b7\u5f97\u7684DNA\u8f7d\u91cf\uff1b\uff08B\uff09\u53cc\u94feDNA\u4fee\u9970\uff1b\uff08C\uff09\u7eb3\u7c73\u63a2\u9488\u8868\u9762\u5e26FAM\u8367\u5149\u6807\u7b7eDNA\u7684\u91ca\u653e\u91cf\uff1b\uff08D\uff09\u76ee\u6807DNA\u8bf1\u5bfc\u5e26FAM\u6807\u7b7eDNA\u91ca\u653e\u8fc7\u7a0b\u7684\u8367\u5149\u76d1\u6d4b<\/p>\n

\u56fe<\/b><\/strong>4. INDEBT\u83b7\u5f97\u7684SNA\u7528\u4e8e\u9ad8\u5bc6\u5ea6\u6838-\u536b\u661f\u7ec4\u88c5<\/b><\/strong><\/p>\n

\"\"<\/p>\n

\uff08A\uff09\u7ec4\u88c5\u7ed3\u679c\u7684\u51dd\u80f6\u7535\u6cf3\u8868\u5f81\uff1b\uff08B\uff09\u57fa\u4e8eTEM\u7684\u7ec4\u88c5\u4ea7\u7269\u536b\u661f\u8ba1\u6570\uff1b\uff08C-D\uff09\u4ee5\u51b7\u51bb\u548cINDEBT\u5236\u5907\u7684DNA\u529f\u80fd\u5316AuNP\u548cAuNR\uff08\u91d1\u7eb3\u7c73\u68d2\uff09\u4e3a\u6838\u7ec4\u88c5\u5f97\u5230\u7684\u6838-\u536b\u661f\u7ed3\u6784TEM\u56fe\u50cf<\/p>\n

\u3010<\/b><\/strong>\u5c0f\u7ed3<\/b><\/strong>\u3011<\/b><\/strong><\/p>\n

\u6587\u7ae0\u5229\u7528\u4e01\u9187\u5f15\u8d77\u7684\u77ac\u95f4\u8131\u6c34\uff08INDEBT\uff09\u5b9e\u73b0SNA\u7684\u79d2\u901f\u751f\u6210\u3002\u8be5\u65b9\u6cd5\u4e0d\u9700\u8981\u7279\u6b8a\u7684\u8bbe\u5907\u3001\u82db\u523b\u7684\u6761\u4ef6\u548c\u96be\u4ee5\u638c\u63e1\u7684\u6280\u5de7\uff0c\u56e0\u800c\u53ef\u671b\u8fdb\u4e00\u6b65\u6269\u5927\u5236\u5907\u89c4\u6a21\u3002\u65b0\u7b56\u7565\u4e0d\u4ec5\u51cf\u5c0f\u4e86SNA\u5236\u5907\u8fc7\u7a0b\u7684\u65f6\u95f4\u548c\u5931\u8d25\u7387\uff0c\u800c\u4e14\u4f7fDNA\u63a5\u679d\u5bc6\u5ea6\u8fbe\u5230\u4e86\u65b0\u8bb0\u5f55\u3002\u8fd9\u4e00\u8fdb\u5c55\u5bf9\u4fc3\u8fdbDNA\u7eb3\u7c73\u6280\u672f\u7684\u5feb\u901f\u53d1\u5c55\u548cSNA\u7684\u5e7f\u6cdb\u5e94\u7528\u5341\u5206\u53ca\u65f6\u3002\u8be5\u5de5\u4f5c\u6240\u5b9e\u73b0\u7684\u5177\u6709\u6781\u9ad8DNA\u5bc6\u5ea6\u7684SNA\u5c06\u9f13\u52b1\u4eba\u4eec\u8fdb\u4e00\u6b65\u63a2\u7d22\u5176\u72ec\u7279\u7684\u5316\u5b66\u548c\u751f\u7269\u5b66\u6548\u5e94\u3002\u4e01\u9187\u8403\u53d6\u662f\u5206\u5b50\u751f\u7269\u5b66\u5b9e\u9a8c\u5ba4\u7684\u5e38\u89c4\u6280\u672f\uff0c\u5176\u5728SNA\u5408\u6210\u4e2d\u5c55\u793a\u7684\u65b0\u7528\u9014\u9884\u793a\u4e86INDEBT\u5728\u5316\u5b66\/\u7eb3\u7c73\u6280\u672f\u4e2d\u8fdb\u4e00\u6b65\u5e94\u7528\u7684\u53ef\u80fd\u6027\u3002<\/p>\n

 <\/p>\n

\u672c\u6587\u7b2c\u4e00\u4f5c\u8005\u4e3a\u4e2d\u56fd\u79d1\u5b66\u6280\u672f\u5927\u5b66\u5728\u8bfb\u535a\u58eb\u751f\u90dd\u8273\u3002<\/p>\n

 <\/p>\n

\u6587\u732e\u94fe\u63a5<\/b><\/strong>\uff1aFlash Synthesis of Spherical Nucleic Acids with Record DNA Density, J. Am. Chem. Soc.<\/b><\/strong>,\u00a0<\/i><\/em>2021, DOI:10.1021\/jacs.1c00568<\/p>\n

\u672c\u6587\u7531\u6750\u6599\u4eba\u5b66\u672f\u7ec4tt\u4f9b\u7a3f\uff0c\u6750\u6599\u725b\u6574\u7406\u7f16\u8f91\u3002<\/p>\n

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