{"id":208486,"date":"2020-09-02T06:48:06","date_gmt":"2020-09-01T22:48:06","guid":{"rendered":"\/\/m.iemloyee.com\/?p=208486"},"modified":"2020-09-01T22:48:55","modified_gmt":"2020-09-01T14:48:55","slug":"%e4%b8%9c%e5%8d%97%e5%a4%a7%e5%ad%a6%e5%88%98%e5%bf%85%e6%88%90%e5%92%8c%e5%90%95%e6%9e%97%e8%8e%89sci-adv-%ef%bc%9a%e7%bb%86%e8%83%9e%e5%a4%96%e5%9b%8a%e6%b3%a1%e5%8c%85%e8%a3%b9il-10%e4%bd%9c","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=208486","title":{"rendered":"\u4e1c\u5357\u5927\u5b66\u5218\u5fc5\u6210\u548c\u5415\u6797\u8389Sci. Adv.\uff1a\u7ec6\u80de\u5916\u56ca\u6ce1\u5305\u88f9IL-10\u4f5c\u4e3a\u7f3a\u8840\u6027AKI\u7684\u65b0\u578b\u7eb3\u7c73\u7597\u6cd5"},"content":{"rendered":"<p>\u3010<strong><b>\u5f15\u8a00<\/b><\/strong>\u3011<\/p>\n<p>\u6025\u6027\u80be\u635f\u4f24\uff08AKI\uff09\u662f\u7531\u591a\u79cd\u56e0\u7d20\u5f15\u8d77\u7684\u80be\u529f\u80fd\u5feb\u901f\u4e0b\u964d\u7684\u5371\u6025\u91cd\u75c7\uff0c\u5728\u4f4f\u9662\u60a3\u8005\u4e2d\u5177\u6709\u9ad8\u53d1\u75c5\u7387\u3001\u9ad8\u6b7b\u4ea1\u7387\u7b49\u7279\u70b9\u3002\u6b64\u5916\uff0cAKI\u5e78\u5b58\u8005\u8fdb\u5c55\u4e3a\u6162\u6027\u80be\u810f\u75c5\uff08CKD\uff09\u548c\u7ec8\u672b\u671f\u80be\u75c5\u7684\u98ce\u9669\u663e\u8457\u589e\u9ad8\u3002\u4f46\u662f\uff0c\u76ee\u524d\u5c1a\u65e0\u660e\u786e\u7684\u7597\u6cd5\u6765\u6cbb\u7597AKI\u6216\u963b\u6b62\u5176\u53d1\u5c55\u4e3aCKD\u3002\u767d\u4ecb\u7d2010\uff08IL-10\uff09\u662f\u4e00\u79cd\u529f\u80fd\u5f3a\u5927\u7684\u514d\u75ab\u8c03\u8282\u5242\uff0c\u5177\u6709\u5f3a\u5927\u7684\u6297\u708e\u548c\u7ec4\u7ec7\u518d\u751f\u80fd\u529b\u3002\u5728AKI\u4e2d\uff0c\u7814\u7a76\u8868\u660eIL-10\u53ef\u4ee5\u901a\u8fc7\u9650\u5236\u708e\u75c7\u7ec6\u80de\u56e0\u5b50\u7684\u4ea7\u751f\u548c\u514d\u75ab\u7ec6\u80de\u6d78\u6da6\u6765\u9884\u9632\u7f3a\u8840\uff0c\u987a\u94c2\u6216\u8f93\u5c3f\u7ba1\u6897\u963b\u5f15\u8d77\u7684\u80be\u635f\u4f24\uff0c\u8fd9\u8868\u660eIL-10\u53ef\u80fd\u6210\u4e3aAKI\u7684\u65b0\u578b\u7597\u6cd5\u3002\u7136\u800c\uff0c\u7ec6\u80de\u56e0\u5b50\u6613\u53d7\u5316\u5b66\u548c\u7269\u7406\u56e0\u7d20\u5f71\u54cd\u800c\u4e0d\u7a33\u5b9a\uff0c\u5e76\u4e14\u4e0d\u53ef\u907f\u514d\u5730\u6fc0\u6d3b\u5faa\u73af\u4e2d\u7684\u767d\u7ec6\u80de\uff0c\u8fd9\u53ef\u80fd\u5bfc\u81f4\u60a3\u8005\u4f24\u5bb3\u6216\u964d\u4f4e\u6cbb\u7597\u6548\u679c\u3002\u89e3\u51b3\u8be5\u95ee\u9898\u7684\u5173\u952e\u662f\u63d0\u9ad8IL-10\u7684\u7a33\u5b9a\u6027\u5e76\u9009\u62e9\u6027\u5730\u9776\u5411\u53d7\u635f\u7684\u80be\u810f\u3002\u7ec6\u80de\u5916\u56ca\u6ce1\uff08EVs\uff09\uff0c\u4f8b\u5982\u5916\u6ccc\u4f53\u548c\u5fae\u56ca\u6ce1\uff0c\u662f\u7ec6\u80de\u4ee5\u7ec4\u6210\u578b\u6216\u8bf1\u5bfc\u578b\u65b9\u5f0f\u5206\u6ccc\u7684\u5c0f\u819c\u9897\u7c92\uff08\u5927\u5c0f\u4e3a40\u81f31000 nm\uff09\u3002\u91ca\u653e\u7684\u7ec6\u80de\u5916\u56ca\u6ce1\u901a\u8fc7\u5c06\u6838\u9178\u548c\u86cb\u767d\u8d28\u8f6c\u8fd0\u81f3\u8fdc\u7aef\u6216\u9644\u8fd1\u7684\u7ec6\u80de\u800c\u81ea\u7136\u5730\u5145\u5f53\u7ec6\u80de\u95f4\u7684\u4fe1\u4f7f\u3002\u6700\u8fd1\u7684\u7814\u7a76\u8868\u660e\uff0c\u7ec6\u80de\u5916\u56ca\u6ce1\u4f5c\u4e3a\u5f3a\u5927\u7684\u7eb3\u7c73\u8f7d\u4f53\u8fdb\u884c\u836f\u7269\u8f93\u9001\u5728\u5316\u5b66\u6cbb\u7597\u3001\u57fa\u56e0\u6cbb\u7597\u7b49\u9886\u57df\u5c55\u73b0\u51fa\u5de8\u5927\u7684\u5e94\u7528\u524d\u666f\u3002\u4e0e\u73b0\u6709\u7684\u9012\u9001\u7cfb\u7edf\u76f8\u6bd4\uff0c\u7ec6\u80de\u5916\u56ca\u6ce1\u7684\u72ec\u7279\u4f18\u52bf\u662f\u5176\u5929\u7136\u6765\u6e90\uff0c\u8fd9\u4f7f\u5176\u80fd\u591f\u9003\u907f\u541e\u566c\u4f5c\u7528\uff0c\u5ef6\u957f\u836f\u7269\u5faa\u73af\u534a\u8870\u671f\u5e76\u964d\u4f4e\u514d\u75ab\u539f\u6027\u3002\u56e0\u6b64\uff0c\u57fa\u4e8eEV\u7684\u836f\u7269\u9012\u9001\u7cfb\u7edf\u53ef\u80fd\u662f\u63a8\u52a8IL-10\u7528\u4e8eAKI\u6cbb\u7597\u7684\u65b0\u578b\u7ed9\u836f\u7b56\u7565\u3002<\/p>\n<p>\u3010<strong><b>\u6210\u679c\u7b80\u4ecb<\/b><\/strong>\u3011<\/p>\n<p><strong><b>\u4e1c\u5357\u5927\u5b66\u5218\u5fc5\u6210\u548c\u5415\u6797\u8389<\/b><\/strong>\u62a5\u9053\u4e86\u4e00\u79cd\u4ece\u5de8\u566c\u7ec6\u80de\u884d\u751f\u7684\u8d1f\u8f7dIL-10\u7684\u7ec6\u80de\u5916\u56ca\u6ce1\uff08IL-10+EV\uff09\u7684\u6709\u6548\u5236\u5907\u65b9\u6cd5\uff0c\u5e76\u7814\u7a76\u4e86IL-10+\u7ec6\u80de\u5916\u56ca\u6ce1\u5728\u7f3a\u8840\u6027AKI\u9f20\u6a21\u578b\u4e2d\u7684\u6cbb\u7597\u6548\u679c\u3002IL-10+EV\u80fd\u591f\u589e\u5f3aIL-10\u7684\u7a33\u5b9a\u6027\uff0c\u5e76\u6709\u6548\u5730\u9776\u5411\u635f\u4f24\u7684\u80be\u810f\uff0c\u8fd9\u662f\u7531\u4e8e\u5176\u8868\u9762\u5bcc\u542b\u7c98\u9644\u6210\u5206\u5305\u62ec\u6574\u8054\u86cb\u767d\u03b1<sub>4<\/sub>\u03b2<sub>1<\/sub>\uff0c\u03b1<sub>5<\/sub>\u03b2<sub>1<\/sub>\uff0c\u03b1<sub>L<\/sub>\u03b2<sub>2<\/sub>\u548c\u03b1<sub>M<\/sub>\u03b2<sub>2<\/sub>\u3002IL-10+EV\u7684\u6cbb\u7597\u4e0d\u4ec5\u53ef\u9776\u5411\u80be\u5c0f\u7ba1\u95f4\u8d28\u4e2d\u7684\u5de8\u566c\u7ec6\u80de\uff0c\u800c\u4e14\u53ef\u9776\u5411\u80be\u4e2d\u7684\u80be\u5c0f\u7ba1\u4e0a\u76ae\u7ec6\u80de\uff08TECs\uff09\uff0c\u53ef\u663e\u8457\u6539\u5584\u80be\u810f\u7f3a\u8840\/\u518d\u704c\u6ce8\uff08I\/R\uff09\u635f\u4f24\uff0c\u5e76\u9632\u6b62AKI\u5411CKD\u7684\u8f6c\u53d8\u3002\u5177\u4f53\u800c\u8a00\uff0c\u6587\u7ae0\u663e\u793aIL-10 + EV\u6291\u5236\u4e86\u54fa\u4e73\u52a8\u7269\u96f7\u5e15\u9709\u7d20\uff08mTOR\uff09\u7684\u9776\u6807\uff0c\u56e0\u6b64\u4fc3\u8fdb\u4e86\u7ebf\u7c92\u4f53\u4ee3\u8c22\u4ee5\u7ef4\u6301TEC\u4e2d\u7684\u7ebf\u7c92\u4f53\u7a33\u6001\u3002\u540c\u65f6\uff0cIL-10 + EV\u8fd8\u53ef\u8bf1\u5bfc\u5de8\u566c\u7ec6\u80de\u5411M2\u578b\u8f6c\u5316\uff0c\u4fc3\u8fdb\u80be\u810f\u4fee\u590d\u3002\u8fd9\u4e2a\u53d1\u73b0\u5f3a\u70c8\u652f\u6301\u5c06\u7ec6\u80de\u5916\u56ca\u6ce1\u7528\u4f5cIL-10\u7684\u591a\u529f\u80fd\u9012\u9001\u7cfb\u7edf\uff0c\u5c06\u5176\u4f5c\u4e3a\u6cbb\u7597\u7f3a\u8840\u6027AKI\u7684\u6709\u6548\u7b56\u7565\u3002\u8be5\u6210\u679c\u4ee5\u9898\u4e3a<strong><b>\u201cExtracellular vesicle\u2013encapsulated IL-10 as novel nanotherapeutics against ischemic AKI\u201d<\/b><\/strong>\u53d1\u8868\u5728<strong><b>Sci. Adv.<\/b><\/strong><strong><b>\u4e0a<\/b><\/strong>\u3002<\/p>\n<p><strong><b>\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/b><\/strong><\/p>\n<p><strong><b>\u56fe1.<\/b><\/strong><strong><b>IL-10+\u7ec6\u80de\u5916\u56ca\u6ce1\u7684\u5236\u5907\u548c\u8868\u5f81<\/b><\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-208487\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2020\/08\/r1-3.jpg\" alt=\"\" width=\"600\" height=\"616\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2020\/08\/r1-3.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2020\/08\/r1-3-292x300.jpg 292w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>\uff08A\uff09\u7528RFP\u6807\u8bb0\u7684IL-10\u8f6c\u67d3RAW\u7ec6\u80de\uff0c\u7136\u540e\u7528\u5730\u585e\u7c73\u677e\u523a\u6fc0<\/p>\n<p>\uff08B\uff09\u5728\u5de5\u7a0bRAW\u7ec6\u80de\u4e2d\u5bf9IL-10\uff08\u7eff\u8272\uff09\u548cEV\u6807\u8bb0CD63\uff08\u7ea2\u8272\uff09\u8fdb\u884c\u514d\u75ab\u67d3\u8272<\/p>\n<p>\uff08C\uff09\u5728IL-10+\u7ec6\u80de\u5916\u56ca\u6ce1\u4e2d\u5bf9EV\u76f8\u5173\u7684\uff08Alix\uff0cCD63\u548cCD81\uff09\u548c\u5de8\u566c\u7ec6\u80de\u76f8\u5173\u7684\u6807\u5fd7\u7269\uff08CD68\u548cCD206\uff09\u8fdb\u884c\u86cb\u767d\u8d28\u5370\u8ff9\u5206\u6790<\/p>\n<p>\uff08D\uff09IL-10+\u7ec6\u80de\u5916\u56ca\u6ce1\u7684\u5c3a\u5bf8\u5206\u5e03\u548c\u4ee3\u8868\u6027TEM\u56fe\u50cf<\/p>\n<p>\uff08E\uff09\u663e\u793a\u4ece\u6297\u4f53\u9635\u5217\u5206\u6790\u83b7\u5f97\u7684\u6bcf\u79cd\u7ec6\u80de\u56e0\u5b50\u7684\u86cb\u767d\u8d28\u6c34\u5e73<\/p>\n<p>\uff08F\uff09\u7ec6\u80de\u5916\u56ca\u6ce1\u4e2dIL-10\u7684ELISA\u5206\u6790\uff0c\u4ee5\u53caIL-10+\u7ec6\u80de\u5916\u56ca\u6ce1\u4e2d\u7684IL-10\u548cIL-10\u53d7\u4f53\u7684\u86cb\u767d\u8d28\u5370\u8ff9\u5206\u6790\uff08n = 3\u62166\uff09<\/p>\n<p>\uff08G\uff09\u901a\u8fc7\u5b9e\u65f6\u5b9a\u91cfPCR\u6d4b\u91cfIL-10+\u7ec6\u80de\u5916\u56ca\u6ce1\u4e2d\u7684IL-10 mRNA<\/p>\n<p>\uff08H-1\uff09\u6bd4\u8f83IL-10 +\u7ec6\u80de\u5916\u56ca\u6ce1\u548c\u6e38\u79bbIL-10\u5728\u4e0d\u540c\u6761\u4ef6\u4e0b\u7684\u7a33\u5b9a\u6027\uff0c\u5305\u62ec\u572837\u00b0C\u6216-80\u00b0C\u4e0b\u653e\u7f6e\u4e00\u5468\uff0c\u5728pH 5.5\u6eb6\u6db2\u4e2d12\u5c0f\u65f6<\/p>\n<p><strong><b>\u56fe2.<\/b><\/strong><strong><b>\u80be\u9776\u5411IL-10+EV<\/b><\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-208488\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2020\/08\/r2-3.jpg\" alt=\"\" width=\"600\" height=\"675\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2020\/08\/r2-3.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2020\/08\/r2-3-267x300.jpg 267w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>\uff08A\uff09\u4ee5\u4eb2\u4ee3RAW\u7ec6\u80de\u4e3a\u5bf9\u7167\u7684IL-10+EVs\u86cb\u767d\u8d28\u7ec4\u6210\u7684LC-MS\/MS\u5206\u6790<\/p>\n<p>\uff08B\uff09\u86cb\u767d\u8d28\u5370\u8ff9\u5206\u6790<\/p>\n<p>\uff08C-E\uff09\u4e3a\u4e86\u5206\u6790\u4f53\u5185\u5206\u5e03\uff0c\u7ed9\u5c0f\u9f20\u9759\u8109\u6ce8\u5c04DID\u6807\u8bb0\u7684IL-10+EV\uff08n=3\uff09<\/p>\n<p>\uff08C\uff09\u6ce8\u5c04\u540e6\u300112\u300124\u300148\u548c96\u5c0f\u65f6\uff08\u7f3a\u8840\u65f6\u95f435\u5206\u949f\uff09\u5bf9\u6307\u5b9a\u5668\u5b98\u7684\u8367\u5149\u5f3a\u5ea6\u6210\u50cf<\/p>\n<p>\uff08D\uff09\u7f3a\u884020\u5206\u949f\uff0c28\u5206\u949f\u548c35\u5206\u949f\u7684IRI\u80be\u810f\u572812\u5c0f\u65f6\u65f6\u7684\u8367\u5149\u5f3a\u5ea6\u6210\u50cf<\/p>\n<p>\uff08E\uff09\u4ee3\u8868\u6027\u5171\u805a\u7126\u56fe\u50cf\u663e\u793aDID\u6807\u8bb0\u7684IL-10+EV\u5728\u5c0f\u7ba1\uff08\u5305\u62ec\u8fd1\u7aef\u5c0f\u7ba1\u548c\u8fdc\u7aef\u5c0f\u7ba1\uff09\uff0c\u5185\u76ae\u7ec6\u80de\uff08CD31\uff09\u548c\u5de8\u566c\u7ec6\u80de\uff08CD68\uff09\u4e2d\u7684\u84c4\u79ef<\/p>\n<p>\uff08F-G\uff09H\/R\u8bf1\u5bfc\u7684TECs\u6444\u53d6PKH67\u6807\u8bb0\u7684IL-10+EV\uff08n = 3\uff09<\/p>\n<p><strong><b>\u56fe<\/b><\/strong><strong><b>3.<\/b><\/strong><strong><b>IL-10+EV\u53ef\u9884\u9632\u5c0f\u9f20\u7684\u80be\u810fI\/R\u635f\u4f24<\/b><\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-208489\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2020\/08\/r3-3.jpg\" alt=\"\" width=\"600\" height=\"949\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2020\/08\/r3-3.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2020\/08\/r3-3-190x300.jpg 190w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>\uff08A\uff09\u5b9e\u9a8c\u8bbe\u8ba1\u793a\u610f\u56fe<\/p>\n<p>\uff08B\uff09IL-10+EV\u5bf9\u8840\u6e05\u808c\u9150\u7684\u5f71\u54cd\uff08n = 10\uff09<\/p>\n<p>\uff08C\uff09\u57fa\u4e8eH\uff06E\u67d3\u8272\u7684\u80be\u5c0f\u7ba1\u635f\u4f24\u7684\u91cf\u5316\uff08n = 10\uff09<\/p>\n<p>\uff08D\uff09\u80be\u76ae\u8d28\u548c\u9ad3\u8d28\u7684H\uff06E\u67d3\u8272\u7684\u4ee3\u8868\u6027\u56fe\u50cf<\/p>\n<p>\uff08E-G\uff09TUNEL\u67d3\u8272\u548c\u51cb\u4ea1\u7ec6\u80de\u5b9a\u91cf\u7684\u4ee3\u8868\u6027\u56fe\u50cf\uff08n = 6\uff09<\/p>\n<p>\uff08F-H\uff09\u4ee3\u8868\u6027\u5171\u805a\u7126\u56fe\u50cf\u548cKIM-1+\u5c0f\u7ba1\u7684\u5b9a\u91cf\uff08n = 6\uff09<\/p>\n<p>\uff08I\uff09\u80be\u810f\u7ec4\u7ec7\u4e2dcaspase-3\u7684\u86cb\u767d\u8d28\u5370\u8ff9\u5206\u6790\uff08n = 3\uff09<\/p>\n<p>\uff08J\uff09\u5b9e\u65f6\u5b9a\u91cfPCR\u5206\u6790\u80be\u810f\u7ec4\u7ec7\u4e2d\u708e\u6027\u7ec6\u80de\u56e0\u5b50mRNA\u6c34\u5e73\uff08n = 6\uff09<\/p>\n<p><strong><b>\u56fe<\/b><\/strong><strong><b>4.<\/b><\/strong><strong><b>IL-10+EVs\u6291\u5236mTOR\u4fe1\u53f7\u4f20\u5bfc\u5e76\u8bf1\u5bfc\u7ebf\u7c92\u4f53\u4fdd\u6301\u7ebf\u7c92\u4f53\u7a33\u6001<\/b><\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-208490\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2020\/08\/r4-3.jpg\" alt=\"\" width=\"600\" height=\"822\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2020\/08\/r4-3.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2020\/08\/r4-3-219x300.jpg 219w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>\uff08A\uff09\u80be\u7ec4\u7ec7\u4e2dmTOR\u4fe1\u53f7\u548cLC3\u7684\u86cb\u767d\u8d28\u5370\u8ff9\u5206\u6790\uff08n = 3\uff09<\/p>\n<p>\uff08B\uff09\u80be\u5c0f\u7ba1\u81ea\u566c\u4e8b\u4ef6\u7684\u4ee3\u8868\u6027TEM\u56fe\u50cf<\/p>\n<p>\uff08C-E\uff09\u8bc4\u4f30IL-10 + EV\u5bf9\u57f9\u517b\u7684TEC\u4e2d\u7ebf\u7c92\u4f53\u529f\u80fd\u7684\u5f71\u54cd\uff08n = 3\uff09<\/p>\n<p>\uff08F\uff09\u80be\u5c0f\u7ba1\u4e2d\u7ebf\u7c92\u4f53\u7684\u4ee3\u8868\u6027TEM\u56fe\u50cf<\/p>\n<p>\uff08G\uff09\u80be\u810f\u5207\u7247\u4e2d\u7ec6\u80de\u8272\u7d20C\u6c27\u5316\u9176\u4e9a\u57faI\uff08MT-CO1\uff09\u8868\u8fbe\u7684\u514d\u75ab\u7ec4\u7ec7\u5316\u5b66\u5206\u6790<\/p>\n<p>\uff08H\uff09\u7ebf\u7c92\u4f53\u547c\u5438\u94fe\u590d\u5408\u4f53I\u7684\u9176\u6d3b\u6027\uff08n = 6\uff09<\/p>\n<p><strong><b>\u56fe<\/b><\/strong><strong><b>5<\/b><\/strong><strong><b>.<\/b><\/strong><strong><b>IL-10+EVs\u5f15\u8d77\u80be\u5de8\u566c\u7ec6\u80de\u8868\u578b\u8f6c\u53d8<\/b><\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-208491\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2020\/08\/r5-3.jpg\" alt=\"\" width=\"600\" height=\"514\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2020\/08\/r5-3.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2020\/08\/r5-3-300x257.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>\uff08AB\uff09\u5206\u522b\u7528LPS\uff0cIL-4+IL-13\u6216\u4e0d\u540c\u5242\u91cf\u7684IL-10+EV\u523a\u6fc0BMDM 48\u5c0f\u65f6\uff08 n = 3\uff09<\/p>\n<p>\uff08C-E\uff09IRI\u7ec4\u4e0eIL-10+EV\u7ec4\u4e4b\u95f4\u80be\u5de8\u566c\u7ec6\u80de\u8868\u578b\u53d8\u5316\u7684\u63a2\u8ba8<\/p>\n<p><strong><b>\u56fe<\/b><\/strong><strong><b>6<\/b><\/strong><strong><b>.<\/b><\/strong><strong><b>IL-10+EV\u6291\u5236AKI-CKD\u8f6c\u53d8<\/b><\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-208492\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2020\/08\/r6-2.jpg\" alt=\"\" width=\"600\" height=\"865\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2020\/08\/r6-2.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2020\/08\/r6-2-208x300.jpg 208w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>\uff08A\uff09PAS\u67d3\u8272\u7684\u4ee3\u8868\u6027\u56fe\u50cf\uff08n = 6\uff09<\/p>\n<p>\uff08B\uff09\u77e2\u72b6\u9762Masson\u4e09\u8272\u67d3\u8272\u7684\u4ee3\u8868\u6027\u56fe\u50cf\uff08n = 6\uff09<\/p>\n<p>\uff08C\uff09\u80be\u7ec4\u7ec7\u4e2d\u80f6\u539f\u86cb\u767dI\u548c\u03b1-\u5e73\u6ed1\u808c\u808c\u52a8\u86cb\u767d\uff08\u03b2-SMA\uff09\u7684\u86cb\u767d\u8d28\u5370\u8ff9\u5206\u6790\uff08n = 4\uff09<\/p>\n<p>\uff08D\uff09\u80be\u810f\u5207\u7247\u4e2dCD68<sup>+<\/sup>\u5de8\u566c\u7ec6\u80de\u548cCD3<sup>+<\/sup>T\u7ec6\u80de\u7684\u4ee3\u8868\u6027\u5171\u805a\u7126\u56fe\u50cf\uff08n = 6\uff09<\/p>\n<p>\uff08E\uff09\u7528\u4e8e\u6cbb\u7597\u7f3a\u8840\u6027AKI\u7684IL-10+EV\u5236\u5242\u7684\u793a\u610f\u56fe<\/p>\n<p><strong><b>\u3010<\/b><\/strong><strong><b>\u5c0f\u7ed3<\/b><\/strong><strong><b>\u3011<\/b><\/strong><\/p>\n<p>\u7ec6\u80de\u5916\u56ca\u6ce1\uff08EVs\uff09\u7528\u4f5c\u5929\u7136\u836f\u7269\u9012\u9001\u7cfb\u7edf\u5df2\u5f15\u8d77\u5f3a\u70c8\u7684\u7814\u7a76\u5174\u8da3\u3002\u4f5c\u8005\u62a5\u544a\u4e86\u4e00\u79cd\u901a\u8fc7\u5de5\u7a0b\u5de8\u566c\u7ec6\u80de\u6765\u5236\u9020\u8d1f\u8f7d\u767d\u4ecb\u7d2010\uff08IL-10\uff09\u7684\u7ec6\u80de\u5916\u56ca\u6ce1\uff08IL-10+EV\uff09\u7684\u65b9\u6cd5\uff0c\u7528\u4e8e\u6cbb\u7597\u7f3a\u8840\u6027\u6025\u6027\u80be\u635f\u4f24\uff08AKI\uff09\u3002\u7ec6\u80de\u5916\u56ca\u6ce1\u8f93\u9001\u4e0d\u4ec5\u589e\u5f3a\u4e86IL-10\u7684\u7a33\u5b9a\u6027\uff0c\u800c\u4e14\u8fd8\u63d0\u9ad8\u4e86\u5176\u5bf9\u80be\u810f\u7684\u9776\u5411\u6027\u3002IL-10+EV\u7684\u6cbb\u7597\u53ef\u663e\u8457\u6539\u5584\u7531\u7f3a\u8840\/\u518d\u704c\u6ce8\u635f\u4f24\u5f15\u8d77\u7684\u80be\u5c0f\u7ba1\u635f\u4f24\u548c\u708e\u75c7\uff0c\u5e76\u6709\u6548\u5730\u9632\u6b62\u4e86\u5411\u6162\u6027\u80be\u810f\u75c5\u7684\u8f6c\u53d8\u3002\u4ece\u673a\u5236\u4e0a\u8bb2\uff0cIL-10+EV\u9776\u5411\u80be\u5c0f\u7ba1\u4e0a\u76ae\u7ec6\u80de\uff0c\u5e76\u6291\u5236\u4e86\u96f7\u5e15\u9709\u7d20\u4fe1\u53f7\u4f20\u5bfc\u7684\u54fa\u4e73\u52a8\u7269\u9776\u6807\uff0c\u4ece\u800c\u4fc3\u8fdb\u7ebf\u7c92\u4f53\u81ea\u566c\uff0c\u7ef4\u6301\u7ebf\u7c92\u4f53\u7a33\u6001\u3002\u6b64\u5916\uff0cIL-10+EV\u53ef\u4ee5\u901a\u8fc7\u9776\u5411\u80be\u5c0f\u7ba1\u95f4\u8d28\u4e2d\u7684\u5de8\u566c\u7ec6\u80de\u6709\u6548\u5730\u9a71\u52a8M2\u5de8\u566c\u7ec6\u80de\u6781\u5316\u3002\u7814\u7a76\u8868\u660e\uff0c\u7ec6\u80de\u5916\u56ca\u6ce1\u4f5c\u4e3a\u8f7d\u4f53\u9012\u9001IL-10\u7684\u65b0\u578b\u7eb3\u7c73\u7597\u6cd5\u5728AKI\u6cbb\u7597\u4e2d\u5177\u6709\u5e7f\u9614\u7684\u5e94\u7528\u524d\u666f\u3002<\/p>\n<p><strong><b>\u6587\u732e\u94fe\u63a5<\/b><\/strong>\uff1aExtracellular vesicle\u2013encapsulated IL-10 as novel nanotherapeutics against ischemic AKI. <em><i>Sci. Adv.<\/i><\/em><em><i>, <\/i><\/em><strong><b>2020<\/b><\/strong>, DOI: 10.1126\/sciadv.aaz0748<\/p>\n<p>\u672c\u6587\u7531tt\u4f9b\u7a3f\u7f16\u8f91\u3002<\/p>\n<p>\u5218\u5fc5\u6210\uff0c\u4e1c\u5357\u5927\u5b66\u533b\u5b66\u9662\u9662\u957f\uff0c\u6c5f\u82cf\u7701\u80be\u810f\u75c5\u4e34\u5e8a\u7814\u7a76\u4e2d\u5fc3\u4e3b\u4efb\uff0c\u4e1c\u5357\u5927\u5b66\u80be\u810f\u75c5\u7814\u7a76\u6240\u6240\u957f\uff0c\u4e1c\u5357\u5927\u5b66\u9996\u5e2d\u6559\u6388(\u4e8c\u7ea7)\u3001\u4e3b\u4efb\u533b\u5e08\uff0c\u535a\u58eb\uff08\u540e\uff09\u7814\u7a76\u751f\u5bfc\u5e08\uff0c\u517c\u4efb\u4e2d\u534e\u533b\u5b66\u4f1a\u80be\u810f\u75c5\u5b66\u4f1a\u526f\u4e3b\u4efb\u59d4\u5458\uff0c\u4e2d\u56fd\u80be\u810f\u751f\u7406\u5b66\u4f1a\u526f\u4e3b\u4efb\u59d4\u5458\u7b49\u3002\u8fd1\u5e74\u6765\uff0c\u5218\u5fc5\u6210\u6559\u6388\u56e2\u961f\u4e3b\u8981\u81f4\u529b\u4e8e\u7ec6\u80de\u5916\u56ca\u6ce1\u5728\u80be\u810f\u75be\u75c5\u4e2d\u7684\u57fa\u7840\u4e0e\u5e94\u7528\u7814\u7a76\uff0c\u53d6\u5f97\u4e86\u4e00\u7cfb\u5217\u5177\u6709\u56fd\u9645\u5f71\u54cd\u529b\u7684\u539f\u521b\u6027\u6210\u679c\uff1a\uff081\uff09\u53d1\u73b0\u4e86\u7ec6\u80de\u5916\u56ca\u6ce1\u4ecb\u5bfc\u7684\u7ec6\u80de\u95f4\u901a\u8baf\u5728\u80be\u5c0f\u7ba1\u95f4\u8d28\u708e\u75c7\u5f62\u6210\u4e2d\u7684\u91cd\u8981\u4f5c\u7528 [1-4]\uff1b\uff082\uff09\u5f00\u5c55\u4e86\u57fa\u4e8e\u7ec6\u80de\u5916\u56ca\u6ce1\u7684\u80be\u810f\u65b0\u578b\u9776\u5411\u6cbb\u7597\u7814\u7a76 [5-8]\u3002\u8bfe\u9898\u7ec4\u5148\u540e\u5f00\u53d1\u4e86\u5229\u7528\u5de8\u566c\u7ec6\u80de\u6e90\u7ec6\u80de\u5916\u56ca\u6ce1\u9012\u9001\u5730\u585e\u7c73\u677e\u548c\u767d\u4ecb\u7d20-10\u7684\u65b0\u578b\u7eb3\u7c73\u7597\u6cd5\u7528\u4e8e\u80be\u810f\u708e\u75c7\u7684\u6cbb\u7597\u3002\u540c\u65f6\u8fd8\u62a5\u9053\u4e86\u5229\u7528\u4e2d\u7a7a\u7ea4\u7ef4\u7cfb\u7edf\u5927\u89c4\u6a21\u5236\u5907\u95f4\u5145\u8d28\u5e72\u7ec6\u80de\u6e90\u5916\u6ccc\u4f53\u7684\u65b0\u65b9\u6cd5\u3002\u76f8\u5173\u5de5\u4f5c\u5df2\u7533\u8bf7\u56fd\u5185\u56fd\u9645\u4e13\u52293\u9879\uff0c\u76ee\u524d\u6388\u67431\u9879\uff0c\u76f8\u5173\u8f6c\u5316\u53ca\u63a8\u5e7f\u6b63\u5728\u8fdb\u884c\u4e2d\u3002<\/p>\n<ul>\n<li>Lv LL, Feng Y, Wu M, Wang B, Li ZL, Zhong X, Wu WJ, Chen J, Ni HF, Tang TT, Tang RN, Lan HY, Liu BC. Exosomal miRNA-19b-3p of tubular epithelial cells promotes M1 macrophage activation in kidney injury. Cell Death Differ. 2020;27(1):210-226.<\/li>\n<li>Lv LL, Feng Y, Wen Y, Wu WJ, Ni HF, Li ZL, Zhou LT, Wang B, Zhang JD, Crowley SD, Liu BC. Exosomal CCL2 from Tubular Epithelial Cells Is Critical for Albumin-Induced Tubulointerstitial Inflammation. J Am Soc Nephrol. 2018;29(3):919-935.<\/li>\n<li>Li ZL, Lv LL, Tang TT, Wang B, Feng Y, Zhou LT, Cao JY, Tang RN, Wu M, Liu H, Crowley SD, Liu BC. HIF-1\u03b1 inducing exosomal microRNA-23a expression mediates the cross-talk between tubular epithelial cells and macrophages in tubulointerstitial inflammation. Kidney Int. 2019;95(2):388-404.<\/li>\n<li>Wang B, Wang ZM, Ji JL, Gan W, Zhang A, Shi HJ, Wang H, Lv L, Li Z, Tang T, Du J, Wang XH, Liu BC. Macrophage-Derived Exosomal Mir-155 Regulating Cardiomyocyte Pyroptosis and Hypertrophy in Uremic Cardiomyopathy. JACC Basic Transl Sci. 2020;5(2):148-166.<\/li>\n<li>Tang TT, Wang B, Wu M, Li ZL, Feng Y, Cao JY, Yin D, Liu H, Tang RN, Crowley SD, Lv LL, Liu BC, Extracellular vesicle\u2013encapsulated IL-10 as novel nanotherapeutics against ischemic AKI. Sci Adv. 2020;6:eaaz0748.<\/li>\n<li>Tang TT, Lv LL, Wang B, Cao JY, Feng Y, Li ZL, Wu M, Wang FM, Wen Y, Zhou LT, Ni HF, Chen PS, Gu N, Crowley SD, Liu BC. Employing Macrophage-Derived Microvesicle for Kidney-Targeted Delivery of Dexamethasone: An Efficient Therapeutic Strategy against Renal Inflammation and Fibrosis. Theranostics. 2019;9(16):4740-4755.<\/li>\n<li>Tang TT, Wang B, Lv LL, Liu BC. Extracellular vesicle-based Nanotherapeutics: Emerging frontiers in anti-inflammatory therapy. Theranostics. 2020;10(18):8111-8129.<\/li>\n<li>Cao J, Wang B, Tang T, Lv L, Ding Z, Li Z, Hu R, Wei Q, Shen A, Fu Y, Liu B. Three-dimensional culture of MSCs produces exosomes with improved yield and enhanced therapeutic efficacy for cisplatin-induced acute kidney injury. Stem Cell Res Ther. 2020;11(1):206.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>\u3010\u5f15\u8a00\u3011 \u6025\u6027\u80be\u635f\u4f24\uff08AKI\uff09\u662f\u7531\u591a\u79cd\u56e0\u7d20\u5f15\u8d77\u7684\u80be\u529f\u80fd\u5feb\u901f\u4e0b\u964d\u7684\u5371\u6025\u91cd\u75c7\uff0c\u5728\u4f4f\u9662\u60a3\u8005\u4e2d\u5177\u6709\u9ad8\u53d1\u75c5\u7387\u3001\u9ad8\u6b7b\u4ea1\u7387\u7b49\u7279\u70b9\u3002\u6b64\u5916\uff0cAKI\u5e78\u5b58\u8005\u8fdb\u5c55\u4e3a\u6162\u6027\u80be\u810f\u75c5\uff08CKD\uff09&#8230;<\/p>\n","protected":false},"author":7336,"featured_media":208487,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[215],"tags":[4344],"class_list":["post-208486","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-jiedu","tag-4344"],"_links":{"self":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/208486"}],"collection":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/users\/7336"}],"replies":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=208486"}],"version-history":[{"count":2,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/208486\/revisions"}],"predecessor-version":[{"id":208674,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/208486\/revisions\/208674"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/media\/208487"}],"wp:attachment":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=208486"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=208486"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=208486"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}