{"id":204171,"date":"2020-06-29T06:38:10","date_gmt":"2020-06-28T22:38:10","guid":{"rendered":"\/\/m.iemloyee.com\/?p=204171"},"modified":"2020-06-28T21:38:28","modified_gmt":"2020-06-28T13:38:28","slug":"%e4%b8%8a%e4%ba%a4%e5%a4%a7%e4%b8%8a%e6%b5%b7%e5%ba%94%e7%94%a8%e7%89%a9%e7%90%86%e6%89%80-nat-commun-%ef%bc%9a%e5%88%a9%e7%94%a8dna%e6%8a%98%e7%ba%b8%e6%8a%80%e6%9c%af%e8%a1%a8%e4%bd%8d%e6%8d%95","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=204171","title":{"rendered":"\u4e0a\u4ea4\u5927&\u4e0a\u6d77\u5e94\u7528\u7269\u7406\u6240 Nat. Commun.\uff1a\u5229\u7528DNA\u6298\u7eb8\u6280\u672f\u8868\u4f4d\u6355\u83b7\u77ac\u65f6\u7684\u6297\u4f53\u6784\u8c61"},"content":{"rendered":"

\u3010\u80cc\u666f\u4ecb\u7ecd\u3011<\/strong><\/p>\n

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\u3010\u6210\u679c\u7b80\u4ecb\u3011<\/strong><\/p>\n

\u57fa\u4e8e\u6b64\uff0c\u4e0a\u6d77\u4ea4\u901a\u5927\u5b66\u7684\u6a0a\u6625\u6d77\u6559\u6388\u3001\u4e2d\u79d1\u9662\u4e0a\u6d77\u5e94\u7528\u7269\u7406\u7814\u7a76\u6240\u7684\u674e\u5bbe\u7814\u7a76\u5458\u548c\u80e1\u94a7\u7814\u7a76\u5458\uff08\u5171\u540c\u901a\u8baf\u4f5c\u8005\uff09\u7b49\u4eba<\/strong>\u8054\u5408\u62a5\u9053\u4e86\u4e00\u79cd\u4e09\u89d2\u5f62\u7684DNA\u6298\u7eb8\u9aa8\u67b6\uff0c\u5177\u6709\u7279\u5b9a\u4f4d\u7f6e\u7684\u951a\u5b9a\u548c\u7a7a\u95f4\u7ec4\u7ec7\u7684\u4eba\u5de5\u8868\u4f4d\uff0c\u53ef\u5728\u5ba4\u6e29\u4e0b\u6355\u83b7\u514d\u75ab\u7403\u86cb\u767dGs\uff08IgGs\uff09\u7684\u77ac\u65f6\u6784\u8c61\u3002\u5176\u4e2d\uff0cDNA\u6298\u7eb8\u8868\u4f4d\uff08DOEs\uff09\u5141\u8bb8\u8868\u4f4d\u77ac\u95f4\u7684\u7a0b\u5e8f\u5316\u7a7a\u95f4\u5206\u5e03\uff0c\u4ece\u800c\u53ef\u4ee5\u4f7f\u7528\u539f\u5b50\u529b\u663e\u5fae\u955c\uff08AFM\uff09\u5bf9\u529f\u80fd\u590d\u5408\u7269\u8fdb\u884c\u76f4\u63a5\u6210\u50cf\u3002\u5728\u672c\u6587\u4e2d\uff0c\u4f5c\u8005\u5efa\u7acb\u4e86IgG\u4eb2\u548c\u529b\u5bf9\u5355\u5206\u5b50\u6c34\u5e73\u4e0a3-20 nm\u5185\u8868\u4f4d\u6a2a\u5411\u8ddd\u79bb\u7684\u5173\u952e\u4f9d\u8d56\u6027\u3002\u77ac\u65f6\u6784\u8c61\u7684\u9ad8\u901fAFM\u6210\u50cf\u8fdb\u4e00\u6b65\u4e3a\u5728\u5355\u4e00\u4e8b\u4ef6\u4e2d\u4ece\u5355\u4ef7\u5230\u4e8c\u4ef7\u7684IgG\u4eb2\u548c\u529b\u63d0\u4f9b\u4e86\u7ed3\u6784\u548c\u52a8\u6001\u8bc1\u636e\uff0c\u8fd9\u4e3a\u5305\u62ec\u75c5\u6bd2\u4e2d\u548c\u3001\u8bca\u65ad\u68c0\u6d4b\u548c\u764c\u75c7\u514d\u75ab\u7597\u6cd5\u5728\u5185\u7684\u5404\u79cd\u5e94\u7528\u63d0\u4f9b\u4e86\u601d\u8def\u3002\u7814\u7a76\u6210\u679c\u4ee5\u9898\u4e3a\u201cCapturing transient antibody conformations with DNA origami epitopes\u201d<\/strong>\u53d1\u5e03\u5728\u56fd\u9645\u8457\u540d\u671f\u520aNat. Commun.<\/strong>\u4e0a\u3002<\/p>\n

\u3010\u56fe\u6587\u89e3\u8bfb\u3011<\/strong><\/p>\n

\u56fe\u4e00\u3001\u57fa\u4e8eDOE\u7684IgGs\u6355\u83b7<\/strong>
\n \"\"\uff08a\uff09\u75c5\u6bd2\u9897\u7c92\u8868\u9762\u4e0a\u6297\u539f\u51b3\u5b9a\u7c07\u5cf0\u7684\u4e0d\u5747\u5300\u5206\u5e03\u7684\u793a\u610f\u56fe\uff1b<\/p>\n

\uff08b\uff09\u8bbe\u8ba1\u3001\u6a21\u4eff\u75c5\u6bd2\u7684DOEs\u7528\u4e8eIgG\u6355\u83b7\u548c\u7ed3\u5408\uff1b<\/p>\n

\uff08c\uff09\u5229\u7528PeakForce-AFM\u56fe\u50cf\u8fdb\u884c\u57fa\u4e8eDOEs\u7684IgG\u6355\u83b7\uff1b<\/p>\n

\uff08d\uff09\uff08c\uff09\u4e2d\u9ec4\u8272\u865a\u7ebf\u6b63\u65b9\u5f62\u7684\u653e\u5927\u56fe\uff1b<\/p>\n

\uff08e\uff09Fc\u548c\u7ed3\u5408IgG\u7684\u4e24\u4e2aFab\u7ed3\u6784\u57df\u7684\u9ad8\u5206\u8fa8\u7387HS-AFM\u56fe\u3001IgG\u7684\u539f\u5b50\u7ed3\u6784\u3001DOEs\u7ed3\u5408IgG\u4e2d\u4e09\u4e2a\u7ed3\u6784\u57df\u7684\u6a2a\u622a\u9762\u8f6e\u5ed3\u3002<\/p>\n

\u56fe\u4e8c\u3001HS-AFM\u8868\u5f81DOE\u9650\u5236\u7684IgG\u6784\u8c61\u7075\u6d3b\u6027\u548cFab-Fab\u8ddd\u79bb<\/strong>
\n \"\"\uff08a\uff09\u7531\u8bbe\u8ba1\u8ddd\u79bb\u5206\u522b\u4e3a5\u30018\u300110\u548c16 nm\u7684DOEs\u6355\u83b7\u7684\u5355\u4e2aIgGs\u4e0d\u540c\u6784\u8c61\u7684\u793a\u610f\u56fe\uff1b<\/p>\n

\uff08b\uff09\u4e24\u4e2aFab\u7ed3\u6784\u57df\u7684\u91cd\u5fc3\u4e4b\u95f4\u7684\u6d4b\u5f97\u8ddd\u79bb\u4ee5\u53ca\u914d\u5bf9\u8868\u4f4d\u7684\u8bbe\u8ba1\u8ddd\u79bb\u7684\u793a\u610f\u56fe\uff1b<\/p>\n

\uff08c\uff09\u8bbe\u8ba1\u7684digoxin\u8ddd\u79bb\u4e0eIgGs\u4e2dFab\u7684\u6d4b\u5f97\u8ddd\u79bb\u4e4b\u95f4\u7684\u5173\u7cfb\u3002<\/p>\n

\u56fe\u4e09\u3001IgG\u4e0eDOEs\u7ed3\u5408\u7684\u52a8\u529b\u5b66<\/strong>
\n \"\"\uff08a\uff09HS-AFM\u56fe\u50cf\u7528\u4e8e\u4e09\u4e2a\u9636\u6bb5\u7ed3\u5408\u8fc7\u7a0b\u7684\u65f6\u95f4\u6f14\u5316\uff0c\u4ece\u6f02\u79fb\u5230\u5355\u4ef7\u7ed3\u5408\uff0c\u518d\u5206\u522b\u662f\u8bbe\u8ba1\u8ddd\u79bb\u5206\u522b\u4e3a3\u300110\u548c16 nm DOEs\u7684\u4e8c\u4ef7\u7ed3\u5408\uff1b<\/p>\n

\uff08b\uff09\u4e09\u4e2a\u9636\u6bb5\u7ed1\u5b9a\u8fc7\u7a0b\u548c\u76f8\u5e94\u7684HS-AFM\u56fe\u50cf\u7684\u793a\u610f\u56fe\uff1b<\/p>\n

\uff08c\uff09\u5355\u5206\u5b50FRET\u8f68\u8ff9\u548c10 nm\u4f4d\u70b9\u7ed3\u5408\u7684hidden Markov\u6a21\u578b\u62df\u5408\u3002<\/p>\n

\u56fe\u56db\u3001\u5f15\u8d77IgG\u4eb2\u548c\u529b\u7684\u5de5\u7a0bDOEs<\/strong>
\n \"\"\uff08a\uff09IgG-DOE\u7684\u5355\u4ef7\u5230\u4e8c\u4ef7\u7ed3\u5408\u8dc3\u8fc1\u52a8\u529b\u5b66\u7684\u6563\u70b9\u56fe\uff0c\u4ee5\u53caIgG\u7ed3\u5408\u6548\u7387\u7684\u76f4\u65b9\u56fe\u968fDOEs\u4e0a\u8bbe\u8ba1\u8ddd\u79bb\u53d8\u5316\u800c\u53d8\u5316\uff1b<\/p>\n

\uff08b\uff09\u8fc7\u7a0b\u7c92\u5ea6\u7684\u5206\u5b50\u52a8\u529b\u5b66\u8ba1\u7b97\uff1b<\/p>\n

\uff08c\uff09\u4f7f\u7528\u8fc7\u7a0b\u7c92\u5ea6\u5206\u5b50\u52a8\u529b\u5b66\u8ba1\u7b97\u548cDOE\u4e0a\u4e8c\u4ef7\u7ed3\u5408IgG\u7684\u76f8\u5e94HS-AFM\u56fe\u50cf\u3001\u5178\u578b\u7684\u6a21\u62df\u6e38\u79bbIgGs\u6784\u8c61\u3002<\/p>\n

\u3010\u5c0f\u7ed3\u3011<\/strong><\/p>\n

\u7efc\u4e0a\u6240\u8ff0\uff0c\u4f5c\u8005\u8bbe\u8ba1\u4e86DOEs\uff0c\u901a\u8fc7\u5229\u7528DNA\u6298\u7eb8\u6280\u672f\u7684\u7a7a\u95f4\u53ef\u5bfb\u6027\u6765\u6a21\u62df\u8868\u4f4d\u5728\u75c5\u6bd2\u9897\u7c92\u4e0a\u7684\u8ddd\u79bb\u5206\u5e03\u3002DOEs\u7684\u5b9a\u4f4d\u80fd\u529b\u548c\u521a\u5ea6\u4f7fIgG\u80fd\u591f\u5728\u5ba4\u6e29\u4e0b\u51bb\u4f4f\uff0c\u4ee5\u4fbf\u5728\u5355\u5206\u5b50\u6c34\u5e73\u5bf9\u77ac\u65f6\u7684\u529f\u80fd\u6027IgG\u7ed3\u5408\u6784\u8c61\u8fdb\u884c\u9ad8\u5206\u8fa8\u7387\u6210\u50cf\u3002\u901a\u8fc7\u5bf9DOEs\u4e0a\u6297\u539f\u51b3\u5b9a\u7c07\u6a2a\u5411\u8ddd\u79bb\u7684\u53ef\u7f16\u7a0b\u63a7\u5236\uff0c\u53ef\u4ee5\u7cbe\u786e\u786e\u5b9a\u6297\u539f\u51b3\u5b9a\u7c07\u4e0e\u6297\u539f\u51b3\u5b9a\u7c07\u4e4b\u95f4\u7684\u4eb2\u548c\u529b\u3002\u6b64\u5916\uff0c\u8be5DOE\u5e73\u53f0\u8fd8\u652f\u6301HS-AFM\u548csmFRET\u5206\u6790\uff0c\u4ee5\u63a2\u6d4bDOE\u4e0a\u5355\u4e2aIgG\u7ed3\u5408\u7684\u52a8\u529b\u5b66\u3002\u7814\u7a76\u53d1\u73b0IgGs\u53ef\u4ee5\u54cd\u5e94\u4ece\u77ed\u5230\u957f\u7684\u8868\u4f4d\u8ddd\u79bb\uff0c\u91c7\u53d6\u4ece\u9ad8\u7d27\u5bc6\u5ea6\u5230\u8fdc\u8ddd\u79bb\u4f38\u5c55\u7684\u7075\u6d3b\u6784\u8c61\u3002\u91cd\u8981\u7684\u662f\uff0c\u5f53\u4e24\u4e2a\u8868\u4f4d\u95f4\u9694\u7ea610 nm\u65f6\uff0c\u4e8c\u4ef7IgG\u7684\u7ed3\u5408\u52a8\u529b\u5b66\u548c\u6548\u7387\u6700\u9ad8\u3002\u603b\u4e4b\uff0cDOEs\u7684\u53ef\u8bbe\u8ba1\u6027\u548c\u53ef\u7f16\u7a0b\u6027\u63d0\u4f9b\u4e86\u4e00\u79cd\u76f4\u89c2\u7684\u65b9\u6cd5\u6765\u6a21\u4eff\u75c5\u6bd2\u8868\u4f4d\u7684\u5206\u5e03\u3002\u56e0\u6b64\uff0cDOEs\u4e0d\u4ec5\u589e\u52a0\u4e86\u5728\u5355\u5206\u5b50\u6c34\u5e73\u4e0a\u7406\u89e3Ab-Ab\u76f8\u4e92\u4f5c\u7528\u7684\u8bbe\u8ba1\u7a7a\u95f4\uff0c\u800c\u4e14\u4e3a\u514d\u75ab\u5de5\u7a0b\u63d0\u4f9b\u4e86\u6f5c\u5728\u7684\u5f3a\u5927\u5e73\u53f0\u3002<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1aCapturing transient antibody conformations with DNA origami epitopes<\/a><\/strong>.\uff08Nat. Commun.<\/em>, 2020<\/strong>, DOI: 10.1038\/s41467-020-16949-4\uff09<\/p>\n

\u672c\u6587\u7531CQR\u7f16\u8bd1\u3002<\/p>\n

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