{"id":190956,"date":"2019-12-04T06:30:09","date_gmt":"2019-12-03T22:30:09","guid":{"rendered":"\/\/m.iemloyee.com\/?p=190956"},"modified":"2019-12-03T20:51:22","modified_gmt":"2019-12-03T12:51:22","slug":"adv-mater-%e5%8f%8c%e9%94%81%e7%ba%b3%e7%b1%b3%e7%b2%92%e5%ad%90%e6%89%b0%e4%b9%b1pd-1-pd-l1%e9%80%9a%e8%b7%af%e7%94%a8%e4%ba%8e%e6%9c%89%e6%95%88%e8%82%bf%e7%98%a4%e5%85%8d%e7%96%ab%e7%96%97","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=190956","title":{"rendered":"Adv. Mater.: \u53cc\u9501\u7eb3\u7c73\u7c92\u5b50\u6270\u4e71PD-1\/PD-L1\u901a\u8def\u7528\u4e8e\u6709\u6548\u80bf\u7624\u514d\u75ab\u7597\u6cd5"},"content":{"rendered":"

\u3010\u7814\u7a76\u80cc\u666f\u4e0e\u7814\u7a76\u8fdb\u5c55\u3011<\/strong><\/p>\n

CRISPR\/CRISPR\u76f8\u5173\u9176(Cas13a)\u53ef\u4ee5\u5728\u9776\u5411\u8bc6\u522b\u540e\u53ef\u901a\u8fc7\u201c\u9644\u5e26\u6548\u5e94\u201d\u5bf9\u80bf\u7624\u7ec6\u80de\u8fdb\u884c\u9009\u62e9\u6027\u6740\u4f24\uff0c\u8fd9\u4f7f\u5f97\u5176\u5728\u80bf\u7624\u6cbb\u7597\u4e2d\u5177\u6709\u5e7f\u9614\u7684\u5e94\u7528\u524d\u666f\u3002\u7136\u800c\uff0c\u8fd9\u79cd\u201c\u9644\u5e26\u6548\u5e94\u201d\u5e76\u4e0d\u7279\u522b\u9488\u5bf9\u80bf\u7624\u7ec6\u80de\uff0c\u5f53\u5728\u5168\u8eab\u7ed9\u836f\u65f6\u53ef\u80fd\u4f1a\u5f15\u8d77\u5b89\u5168\u95ee\u9898\u3002\u8fd1\u65e5\uff0c\u5357\u5f00\u5927\u5b66\u5218\u9633\u6559\u6388\uff0c\u53f2\u6797\u542f\u6559\u6388\u548c\u5929\u6d25\u533b\u79d1\u5927\u5b66\u5eb7\u6625\u751f\u6559\u6388\uff08\u5171\u540c\u901a\u8baf\u4f5c\u8005\uff09\u5728\u56fd\u9645\u8457\u540d\u671f\u520aAdv. Mater.\u4e0a\u53d1\u8868\u76f8\u5173\u7814\u7a76\u6587\u7ae0\uff0c\u9898\u76ee\u4e3a\u201cDual-Locking Nanoparticles Disrupt the PD-1\/PD-L1 Pathway for Efficient Cancer Immunotherapy\u201d\u3002<\/p>\n

\u8be5\u7814\u7a76\u63cf\u8ff0\u4e86\u4e00\u79cd\u53ef\u9650\u5236CRISPR\/Cas13a\u5bf9\u80bf\u7624\u7ec4\u7ec7\u7279\u5f02\u6027\u6fc0\u6d3b\u7684\u53cc\u9501\u7eb3\u7c73\u9897\u7c92\uff08DLNP\uff09\u3002DLNP\u5177\u6709\u6838-\u58f3\u7ed3\u6784\uff0c\u5176\u4e2dCRISPR\/Cas13a\u7cfb\u7edf\uff08\u8d28\u7c92DNA\uff0cpDNA\uff09\u88ab\u5177\u6709\u53cc\u54cd\u5e94\u805a\u5408\u7269\u58f3\u5c42\u5305\u88f9\u5728\u6838\u5185\u3002\u8be5\u805a\u5408\u7269\u5c42\u8d4b\u4e88DLNP\u5728\u8840\u6db2\u5faa\u73af\u6216\u6b63\u5e38\u7ec4\u7ec7\u4e2d\u589e\u5f3a\u7684\u7a33\u5b9a\u6027\uff0c\u5e76\u4fc3\u8fdbCRISPR\/Cas13a\u7cfb\u7edf\u7684\u7ec6\u80de\u5185\u5316\u548c\u8fdb\u5165\u80bf\u7624\u7ec4\u7ec7\u65f6\u57fa\u56e0\u7f16\u8f91\u7684\u6fc0\u6d3b\u3002\u5728\u4ed4\u7ec6\u7b5b\u9009\u548c\u4f18\u5316\u4ee5\u7a0b\u5e8f\u6027\u6b7b\u4ea1\u914d\u4f531(PD-L1)\u4e3a\u9776\u70b9\u7684CRISPR RNA(crRNA)\u5e8f\u5217\u540e\uff0cDLNP\u663e\u793a\u4e86\u5bf9B16F10\u8377\u7624\u5c0f\u9f20T\u7ec6\u80de\u4ecb\u5bfc\u7684\u6297\u80bf\u7624\u514d\u75ab\u7684\u6709\u6548\u6fc0\u6d3b\u548c\u514d\u75ab\u6291\u5236\u80bf\u7624\u5fae\u73af\u5883\uff08TME\uff09\u7684\u91cd\u5851\uff0c\u4ece\u800c\u663e\u8457\u6291\u5236\u4e86\u80bf\u7624\u7684\u751f\u957f\uff0c\u63d0\u9ad8\u4e86\u8377\u7624\u5c0f\u9f20\u7684\u751f\u5b58\u7387\u3002<\/p>\n

\u3010\u56fe\u6587\u7b80\u4ecb\u3011<\/strong><\/p>\n

\u56fe1 DLNP<\/strong>\u5728\u6709\u6548\u80bf\u7624\u514d\u75ab\u6cbb\u7597\u4e2d\u7684\u793a\u610f\u56fe<\/strong><\/p>\n

\"\"<\/p>\n

\u7c7b\u4f3c\u4e8e\u4ec5\u5728\u4e24\u4e2a\u9501\u90fd\u89e3\u9501\u65f6\u624d\u80fd\u6253\u5f00\u7684\u53cc\u9501\u4fdd\u9669\u7bb1\uff0cDLNP\u53ea\u80fd\u5728\u4f4epHe\u548c\u9ad8H2<\/sub>O2<\/sub>\u6d53\u5ea6\u540c\u65f6\u5b58\u5728\u7684\u5fae\u73af\u5883\u4e2d\u91ca\u653eCRISPR \/ Cas13a\u7cfb\u7edf\u3002\u7528\u8fd9\u79cd\u57fa\u4e8eCRISPR\/Cas13a\u7684PD-1\/PD-L1\u901a\u8def\u9776\u5411\u5242\uff0cDLNP\u6709\u6548\u5730\u6307\u5bfc\u4e86T\u7ec6\u80de\u4ecb\u5bfc\u7684\u6297\u80bf\u7624\u514d\u75ab\u5e94\u7b54\uff0c\u5e76\u5bf9\u514d\u75ab\u6291\u5236\u7684TME\u8fdb\u884c\u4e86\u91cd\u5851\uff0c\u4f7f\u6297\u80bf\u7624\u6548\u679c\u548c\u751f\u5b58\u7387\u663e\u8457\u63d0\u9ad8\uff0c\u4e14\u5bf9\u5c0f\u9f20\u7684\u526f\u4f5c\u7528\u6781\u5c0f\u3002<\/p>\n

\u56fe2 DLNP<\/strong>\u3001H2<\/sub>O2<\/sub>-NP<\/strong>\u548cpH-NP<\/strong>\u7684\u7ed3\u6784\u8868\u5f81<\/strong><\/p>\n

\"\"<\/p>\n

a\uff09\u53cc\u9501\u5b9a\u7eb3\u7c73\u9897\u7c92\uff08DLNP\uff09\u7684\u5408\u6210\u793a\u610f\u56fe\uff1b<\/p>\n

b\uff09DLNP\u7684\u5c3a\u5bf8\u5206\u5e03\u548cTEM\u56fe\u50cf\u3002\u6807\u5c3a\u4e3a200\u7eb3\u7c73\uff1b<\/p>\n

c\uff09\u4e0d\u540c\u6761\u4ef6\u4e0bDLNP\u3001H2<\/sub>O2<\/sub>-NP\u548cpH-NP\u7684Zeta\u7535\u4f4d\uff1b<\/p>\n

d\uff09\u572837\u00b0C\u7684FBS\u6eb6\u6db2\u4e2d\u57f9\u517b30\u5206\u949f\u540e\uff0cDLNP\u3001H2<\/sub>O2<\/sub>-NP\u548cpH-NP\u7684\u975e\u7279\u5f02\u6027\u86cb\u767d\u8d28\u5438\u9644\u91cf\uff1b<\/p>\n

e\uff09\u4e0d\u540c\u6761\u4ef6\u4e0b\u57f9\u517b\u540eDLNP\u7684\u8367\u5149\u5149\u8c31\u3002PEI-PBA\u548cPEI-HPBA\u7528Cy5\u9884\u6807\u8bb0\uff1bmPEG-b-PLys-SGD\/CA\u548cmPEG-b-PLys-SGD\/SA\u7528Cy3\u9884\u6807\u8bb0\u3002\u5728515nm\u7684\u6fc0\u53d1\u6ce2\u957f\u4e0b\u8bb0\u5f55\u5149\u8c31\uff1b<\/p>\n

f\uff09\u6d41\u5f0f\u7ec6\u80de\u4eea\u5b9a\u91cf\u5206\u6790DLNP\uff0cH2<\/sub>O2<\/sub>-NP\u548cpH-NP\u7684\u7ec6\u80de\u5185\u5728\u5316\u7a0b\u5ea6\uff1b<\/p>\n

g\uff09\u5c06DLNP\uff08a\uff09\uff0cH2O2-NP\uff08b\uff09\u548cpH-NP\uff08c\uff09\u5728\u4e0d\u540c\u6761\u4ef6\u4e0b\u4e0e\u80f6\u8d28\u7624\u7ec6\u80de\u5171\u57f9\u517b2 h\u7684\u8367\u5149\u663e\u5fae\u955c\u56fe\u7247\u3002\u7ec6\u80de\u9aa8\u67b6F-\u808c\u52a8\u86cb\u767d\u548c\u7ec6\u80de\u6838\u5206\u522b\u7528\u82e5\u4e39\u660e\u9b3c\u7b14\u73af\u80bd\uff08\u6a59\u8272\uff09\u548c4\uff0c6-\u4e8c\u6c28\u57fa-2-\u82ef\u57fa\u5432\u54da\uff08DAPI\uff09\uff08\u84dd\u8272\uff09\u590d\u67d3\u8272\u3002 \u6807\u5c3a\u4e3a40 \u00b5m\uff1b<\/p>\n

h\uff09\u5728\u542b\u67090\uff05\u621610\uff05\u8840\u6e05\u7684\u57f9\u517b\u57fa\u4e2d\uff0c\u4e0d\u540c\u6761\u4ef6\u4e0b\u7528DLNP\uff0cH2<\/sub>O2<\/sub>-NP\u548cpH-NP\u8f6c\u67d3\u7684U87MG\u7ec6\u80de\u7684\u8367\u5149\u663e\u5fae\u955c\u56fe\u50cf\u3002<\/p>\n

\u56fe3 DLNP<\/strong>\u3001H2<\/sub>O2<\/sub>-NP<\/strong>\u548cpH-NP<\/strong>\u7684\u751f\u7269\u529f\u80fd\u8868\u5f81<\/strong><\/p>\n

\"\"<\/p>\n

a\u2013c\uff09\u5728\u4e0d\u540c\u6761\u4ef6\u4e0b\u7528DLNP\u5904\u7406\u7684B16F10\uff08a\uff09\uff0cGL261\uff08b\uff09\u548c4T1\uff08c\uff09\u7ec6\u80de\u7684RNA\u53d8\u6027\u51dd\u80f6\u3002DLNP\u8d1f\u8f7dCas13a\/PD-L1\uff1b<\/p>\n

d, e\uff09\u5728\u4e0d\u540c\u6761\u4ef6\u4e0b\u7528DLNP\u5904\u7406\u7684B16F10\uff08a\uff09\uff0cGL261\u548c4T1\uff08b\uff09\u7684\u7ec6\u80de\u5b58\u6d3b\u7387\u3002<\/p>\n

\u56fe4 DLNP<\/strong>\u7684\u4f53\u5185\u6297\u80bf\u7624\u4f5c\u7528<\/strong><\/p>\n

\"\"<\/p>\n

a-c\uff09PBS\uff0cNull\uff0cPEI25k<\/sub>\/pDNA\uff0cpH-NP\uff0cH2<\/sub>O2<\/sub>-NP\u548cDLNP\u5904\u7406\u7684\u5c0f\u9f20\u7684\u5e73\u5747\u80bf\u7624\u751f\u957f\u52a8\u529b\u5b66a\uff09\uff0c\u8377\u7624\u5c0f\u9f20\u7684\u5b58\u6d3b\u7387b\uff09\uff0c\u4ee5\u53ca\u5c0f\u9f20\u7684\u4f53\u91cd\u53d8\u5316c\uff09\uff08n = 6\uff09\uff1b<\/p>\n

d-f\uff09PBS\uff0cNull\uff0cPEI25k<\/sub>\/pDNA\uff0cpH-NP\uff0cH2<\/sub>O2<\/sub>-NP\u548cDLNP\u5904\u7406\u7684\u5c0f\u9f20\u7684\u80bf\u7624\u4e2d\u80bf\u7624\u5185\u7684TNF-\u03b1\uff08a\uff09\uff0cIFN-\u03b3\uff08b\uff09\u548cIL-2\uff08c\uff09\u6c34\u5e73\uff1b<\/p>\n

g\uff09PBS\uff0cNull\uff0cPEI25k<\/sub>\uff0cpH-NP\uff0cH2<\/sub>O2<\/sub>-NP\u548cDLNP\u5904\u7406\u7684\u5c0f\u9f20\u4f53\u5185\u80bf\u7624\u5185CD8+ T\u7ec6\u80de\u7684\u6d41\u5f0f\u7ec6\u80de\u7edf\u8ba1\u5206\u6790\uff1b<\/p>\n

h\uff09PBS\uff0cNull\uff0cPEI25k<\/sub>\uff0cpH-NP\uff0cH2<\/sub>O2<\/sub>-NP\u548cDLNP\u5904\u7406\u7684\u5c0f\u9f20\u4f53\u5185\u80bf\u7624\u5185CD8+ T\u7ec6\u80de\u548cCD4+ T\u7ec6\u80de\u7684\u6d41\u5f0f\u7ec6\u80de\u4eea\u56fe\uff1b<\/p>\n

i\uff09\u80bf\u7624\u7684\u4ee3\u8868\u6027\u514d\u75ab\u8367\u5149\u56fe\u50cf\u663e\u793aCD8+ T\u7ec6\u80de\u548cCD4+ T\u7ec6\u80de\u6d78\u6da6\u3002\u6807\u5c3a\u4e3a100 \u03bcm\u3002<\/p>\n

\u56fe5 TME<\/strong>\u4e2dDLNP<\/strong>\u7684\u514d\u75ab\u53cd\u5e94\u5206\u6790<\/strong><\/p>\n

\"\"<\/p>\n

a\uff0cd\uff09PBS\uff0cNull\uff0cPEI25k<\/sub>\uff0cpH-NP\uff0cH2<\/sub>O2<\/sub>-NP\u548cDLNP\u5904\u7406\u7684\u5c0f\u9f20\u4f53\u5185\u80bf\u7624\u5185M2\u7ec6\u80de\u7684\u6d41\u5f0f\u7ec6\u80de\u4eea\u56fea\uff09\uff0c\u548c\u7edf\u8ba1\u7ed3\u679cd\uff09\uff1b<\/p>\n

b\uff0ce\uff09PBS\uff0cNull\uff0cPEI25k<\/sub>\uff0cpH-NP\uff0cH2<\/sub>O2<\/sub>-NP\u548cDLNP\u5904\u7406\u7684\u5c0f\u9f20\u4f53\u5185\u80bf\u7624\u5185M1\u7ec6\u80de\u7684\u6d41\u5f0f\u7ec6\u80de\u4eea\u56feb\uff09\uff0c\u548c\u7edf\u8ba1\u7ed3\u679ce\uff09\uff1b<\/p>\n

c\uff0cf\uff09PBS\uff0cNull\uff0cPEI25k<\/sub>\uff0cpH-NP\uff0cH2<\/sub>O2<\/sub>-NP\u548cDLNP\u5904\u7406\u7684\u5c0f\u9f20\u4f53\u5185\u80bf\u7624\u5185\u9ad3\u7cfb\u6291\u5236\u7ec6\u80de\u7684\u6d41\u5f0f\u7ec6\u80de\u4eea\u56fec\uff09\uff0c\u548c\u7edf\u8ba1\u7ed3\u679cf\uff09\uff1b<\/p>\n

g\uff09PBS\uff0cNull\uff0cPEI25k<\/sub>\/pDNA\uff0cpH-NP\uff0cH2<\/sub>O2<\/sub>-NP\u548cDLNP\u5904\u7406\u7684\u5c0f\u9f20\u7684\u80bf\u7624\u4e2d\u80bf\u7624\u5185\u7684IL-12\u6c34\u5e73\u3002<\/p>\n

\u56fe6 DLNP<\/strong>\u5bf9CD8+T<\/strong>\u7ec6\u80de\u7f3a\u5931\u7684B16F10<\/strong>\u8377\u7624\u5c0f\u9f20\u548c4T1\/B16F10<\/strong>\u5171\u8377\u7624\u5c0f\u9f20\u4f53\u5185\u6297\u80bf\u7624\u4f5c\u7528\u7684\u7814\u7a76<\/strong><\/p>\n

\"\"<\/p>\n

a-d\uff09 DLNP\u5904\u7406\u7684\u6b63\u5e38\u8377\u7624\u5c0f\u9f20\u53caCD8+\u963b\u65ad\u8377\u7624\u5c0f\u9f20\u7684\u5e73\u5747\u80bf\u7624\u751f\u957f\u52a8\u529b\u5b66a\uff09\uff0c\u5b58\u6d3b\u7387b\uff09\uff0c\u4ee5\u53ca\u5c0f\u9f20\u7684\u4f53\u91cd\u53d8\u5316d\uff09\uff1b<\/p>\n

c\uff09\u80bf\u7624\u7684\u4ee3\u8868\u6027\u514d\u75ab\u8367\u5149\u56fe\u50cf\u663e\u793aCD8 + T\u7ec6\u80de\u548cCD4 + T\u7ec6\u80de\u6d78\u6da6\u3002 \u6bd4\u4f8b\u5c3a\u4e3a100 \u03bcm;<\/p>\n

e\uff094T1\uff08\u53f3\uff09\u548cB16F10\uff08\u5de6\uff09\u5171\u80b2C57BL\/6\u5c0f\u9f20\u793a\u610f\u56fe\uff1b<\/p>\n

f\uff09\u5171\u8d1f\u8f7d\u5c0f\u9f20\u7684\u80bf\u7624\u751f\u957f\u66f2\u7ebf\uff1b<\/p>\n

g\uff09\u5206\u522b\u7528PBS\u548cDLNP\u5904\u7406\u7684\u5c0f\u9f204T1\u548cB16F10\u80bf\u7624\u4e2dCD8+T\u7ec6\u80de\u548cCD4+T\u7ec6\u80de\u7684\u4ee3\u8868\u6027\u6d41\u5f0f\u7ec6\u80de\u4eea\u56fe\u3002<\/p>\n

\u3010\u5c0f\u7ed3\u3011<\/strong><\/p>\n

\u8be5\u7814\u7a76\u5f00\u53d1\u4e86\u4e00\u79cd\u53cc\u9501\u7eb3\u7c73\u7c92\u5b50\uff0c\u5b83\u53ef\u4ee5\u9650\u5236CRISPR\/Cas13a\u5728\u80bf\u7624\u7ec4\u7ec7\u4e2d\u7684\u6fc0\u6d3b\u3002\u91c7\u7528\u805a\u4e59\u4e8c\u9187\uff08PEG\uff09\u4e3a\u57fa\u7684\u805a\u5408\u7269\u5916\u58f3\uff0c\u7ecf\u9759\u8109\u6ce8\u5c04\u540e\uff0cDLNP\u5177\u6709\u826f\u597d\u7684\u8840\u6db2\u5faa\u73af\u7a33\u5b9a\u6027\u3002\u66f4\u91cd\u8981\u7684\u662f\uff0cDLNP\u53ea\u80fd\u5728\u4f4eph\u548c\u9ad8H2<\/sub>O2<\/sub>\u6d53\u5ea6\u7684\u5fae\u73af\u5883\u4e2d\u91ca\u653eCRISPR\/Cas13a\u7cfb\u7edf\u3002\u8fd9\u4e00\u7279\u6027\u663e\u8457\u6539\u5584\u4e86CRISPR\/Cas13a\u7cfb\u7edf\u5728\u80bf\u7624\u90e8\u4f4d\u7684\u5bcc\u96c6\uff0c\u63d0\u9ad8\u4e86CRISPR\/Cas13a\u7cfb\u7edf\u7684\u57fa\u56e0\u7f16\u8f91\u6548\u7387\uff0c\u51cf\u5c11\u4e86CRISPR\/Cas13a\u5728\u6b63\u5e38\u7ec4\u7ec7\u4e2d\u7684\u610f\u5916\u6fc0\u6d3b\u6240\u5f15\u8d77\u7684\u526f\u4f5c\u7528\u3002\u901a\u8fc7DLNP\u5bf9CRISPR\/Cas13a\u6d3b\u5316\u7684\u7cbe\u786e\u63a7\u5236\uff0c\u9996\u6b21\u5b9e\u73b0\u4e86CRISPR\/Cas13a\u7cfb\u7edf\u5728\u80bf\u7624\u514d\u75ab\u6cbb\u7597\u4e2d\u7684\u5e94\u7528\u3002<\/p>\n

\u5168\u8eab\u7ed9\u836fDLNP\/Cas13a\/PD-L1\u540e\uff0cB16F10\u8377\u7624\u5c0f\u9f20\u7684\u80bf\u7624\u751f\u957f\u901f\u7387\u663e\u8457\u51cf\u7f13\uff0c\u751f\u5b58\u7387\u660e\u663e\u63d0\u9ad8\u3002\u56e0\u6b64\uff0c\u6211\u4eec\u7684\u7814\u7a76\u4e3a\u7cbe\u786e\u63a7\u5236CRISPR\/Cas13a\u7cfb\u7edf\u6fc0\u6d3b\u6291\u5236\u80bf\u7624\u751f\u957f\u63d0\u4f9b\u4e86\u4e00\u79cd\u5b89\u5168\u6709\u6548\u7684\u65b9\u6cd5\u3002\u8003\u8651\u5230\u514d\u75ab\u6548\u5e94\u7ec6\u80de\u6291\u5236\u9014\u5f84\u7684\u591a\u6837\u6027\uff0c\u53ef\u4ee5\u901a\u8fc7\u66ff\u6362\u7279\u5f02\u6027crRNA\u7684\u9776\u5411\u57fa\u56e0\u800c\u8fdb\u884c\u7684\u8fdb\u4e00\u6b65\u5f00\u53d1\u53ef\u80fd\u4f7fDLNP\u6210\u4e3a\u5feb\u901f\u5f00\u53d1\u5b89\u5168\u6709\u6548\u7684\u764c\u75c7\u514d\u75ab\u7597\u6cd5\u7684\u901a\u7528\u5e73\u53f0\u3002<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1a<\/strong>Dual-Locking Nanoparticles Disrupt the PD-1\/PD-L1 Pathway for Efficient Cancer Immunotherapy, 2019, Adv. Mater. DOI: 10.1002\/adma.201905751.<\/p>\n

\u56e2\u961f\u4ecb\u7ecd<\/strong>\uff1a<\/p>\n

\u5218\u9633\u535a\u58eb\uff0c\u7814\u7a76\u5458\uff0c2006\u5e74\u6bd5\u4e1a\u4e8e\u5357\u5f00\u5927\u5b66\u5316\u5b66\u4e13\u4e1a\uff0c\u83b7\u5b66\u58eb\u5b66\u4f4d\u3002\u540c\u5e74\u8003\u5165\u5357\u5f00\u5927\u5b66\u9ad8\u5206\u5b50\u5316\u5b66\u7814\u7a76\u6240\uff0c2011\u5e74\u6bd5\u4e1a\uff0c\u83b7\u9ad8\u5206\u5b50\u5316\u5b66\u535a\u58eb\u5b66\u4f4d\u3002\u6bd5\u4e1a\u540e\u8d74\u7f8e\u5728\u52a0\u5dde\u5927\u5b66\u6d1b\u6749\u77f6\u5206\u6821\u5316\u5b66\u5de5\u7a0b\u4e0e\u5206\u5b50\u751f\u7269\u5de5\u7a0b\u7cfb\u6df1\u9020\uff0c\u4e8e2016\u5e74\u83b7\u5316\u5b66\u5de5\u7a0b\u535a\u58eb\u5b66\u4f4d\u3002\u4e3b\u8981\u4ece\u4e8b\u9ad8\u5206\u5b50\u7eb3\u7c73\u836f\u7269\u8f7d\u4f53\u53ca\u751f\u7269\u6d3b\u6027\u7eb3\u7c73\u6750\u6599\u7684\u7814\u7a76\u3002\u4ee3\u8868\u6027\u7814\u7a76\u6210\u679c\u5305\u62ec\uff1a\u5efa\u7acb\u4e86\u7eb3\u7c73\u590d\u5408\u9176\u7684\u53ef\u63a7\u6784\u5efa\u65b9\u6cd5\uff0c\u5b9e\u73b0\u4e86\u591a\u79cd\u86cb\u767d\u4f53\u5185\u548c\u80de\u5185\u7684\u534f\u540c\u9012\u9001\uff1b\u5efa\u7acb\u4e86\u591a\u79cd\u53ef\u52a8\u6001\u9002\u5e94\u4f53\u5185\u73af\u5883\u7684\u7eb3\u7c73\u8f7d\u4f53\uff0c\u663e\u8457\u63d0\u5347\u4e86\u836f\u7269\u5bf9\u80bf\u7624\u7684\u5bcc\u96c6\u6548\u7387\uff1b\u63d0\u51fa\u901a\u8fc7\u7eb3\u7c73-\u751f\u7269\u754c\u9762\u76f8\u4e92\u4f5c\u7528\u76f4\u63a5\u5e72\u9884\u751f\u7269\u8fc7\u7a0b\u7684\u65b0\u601d\u8def\uff0c\u6784\u5efa\u591a\u79cd\u5177\u6709\u751f\u7269\u6d3b\u6027\u8868\u9762\u7684\u7eb3\u7c73\u9897\u7c92\uff0c\u4e3a\u764c\u75c7\u514d\u75ab\u6cbb\u7597\u53ca\u963f\u5c14\u5179\u6d77\u9ed8\u75c7\u7684\u6cbb\u7597\u63d0\u4f9b\u4e86\u5168\u65b0\u7684\u601d\u8def\u3002\u76f8\u5173\u6210\u679c\u5728Nat. Nanotechnol. (1), Adv. Mater. (3), Nat. Commun. (1), Angew. Chem. Int. Ed. (2), Nano Lett. (2), Adv. Sci. (1) \u7b49\u671f\u520a\u53d1\u8868\u8bba\u658740\u4f59\u7bc7\u30022016\u5e74\u5165\u9009\u4e2d\u7ec4\u90e8\u201c\u9752\u5e74\u5343\u4eba\u201d\uff0c2018\u5e74\u4ee5\u9996\u5e2d\u79d1\u5b66\u5bb6\u8eab\u4efd\u627f\u62c5\u79d1\u6280\u90e8\u91cd\u70b9\u7814\u53d1\u8ba1\u5212\u201c\u7eb3\u7c73\u79d1\u6280\u201d\u9752\u5e74\u9879\u76ee\u3002<\/p>\n

\u63a8\u8350\u6587\u732e<\/strong>\uff1a<\/p>\n

    \n
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