{"id":187583,"date":"2019-10-19T06:30:25","date_gmt":"2019-10-18T22:30:25","guid":{"rendered":"\/\/m.iemloyee.com\/?p=187583"},"modified":"2019-10-19T18:10:40","modified_gmt":"2019-10-19T10:10:40","slug":"nature-reviews-materials-%e7%bb%bc%e8%bf%b0%ef%bc%9a%e5%9f%ba%e5%9b%a0%e7%bb%84%e7%bc%96%e8%be%91%e6%9c%ba%e5%99%a8%e4%bd%93%e5%86%85%e9%80%92%e9%80%81%e7%9a%84%e5%b7%a5%e7%a8%8b%e6%9d%90%e6%96%99","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=187583","title":{"rendered":"Nature Reviews Materials \u7efc\u8ff0\uff1a\u57fa\u56e0\u7ec4\u7f16\u8f91\u673a\u5668\u4f53\u5185\u9012\u9001\u7684\u5de5\u7a0b\u6750\u6599"},"content":{"rendered":"

Nature Reviews Materials <\/strong>\u7efc\u8ff0\uff1a\u57fa\u56e0\u7ec4\u7f16\u8f91\u673a\u5668\u4f53\u5185\u9012\u9001\u7684\u5de5\u7a0b\u6750\u6599<\/strong><\/p>\n

\u3010\u80cc\u666f\u4e0e\u7814\u7a76\u8fdb\u5c55\u3011<\/strong><\/p>\n

\u57fa\u56e0\u7ec4\u7f16\u8f91\u6280\u672f\uff0c\u5982CRISPR-CAS9\uff0c\u6709\u671b\u6cbb\u7597\u8bb8\u591a\u65e0\u6cd5\u6cbb\u6108\u7684\u9057\u4f20\u75be\u75c5\u3002\u76ee\u524d\u79bb\u4f53\u57fa\u56e0\u7ec4\u7f16\u8f91\u5df2\u7ecf\u53d6\u5f97\u4e86\u5f88\u5927\u7684\u8fdb\u5c55\u3002\u4f46\u662f\uff0c\u6709\u6548\u3001\u5b89\u5168\u3001\u53ef\u9776\u5411\u7684\u4f53\u5185\u4f20\u9012\u7cfb\u7edf\u7684\u5f00\u53d1\u4ecd\u7136\u5b58\u5728\u74f6\u9888\u3002\u5728\u4f53\u5185\u9ad8\u6548\u5b89\u5168\u7684\u8fdb\u884c\u53ef\u63a7\u57fa\u56e0\u7ec4\u7f16\u8f91\u9700\u8981\u65b0\u7684\u6750\u6599\u3001\u4f20\u9012\u65b9\u6cd5\u548c\u63a7\u5236\u673a\u5236\u3002\u56e0\u6b64\uff0c\u521b\u65b0\u7814\u53d1\u4f53\u5185\u9012\u9001\u7cfb\u7edf\u4ee5\u514b\u670d\u76ee\u524d\u7f16\u8f91\u6548\u7387\u4f4e\u3001\u975e\u9776\u5411\u6027\u3001\u4f4e\u5b89\u5168\u6027\u4ee5\u53ca\u7f3a\u4e4f\u7ec6\u80de\u548c\u7ec4\u7ec7\u7279\u5f02\u6027\u7b49\u95ee\u9898\u662f\u8be5\u9886\u57df\u7684\u91cd\u8981\u8bfe\u9898\u3002<\/p>\n

\u8fd1\u65e5\uff0c\u83b1\u65af\u5927\u5b66\u751f\u7269\u5de5\u7a0b\u7cfb\u5305\u521a\u6559\u6388\u5728Nature Reviews Materials\u4e0a\u53d1\u8868\u9898\u4e3a\u201cEngineered materials for in\u00a0vivo delivery of genome-editing machinery\u201d\u7684\u7efc\u8ff0\u6587\u7ae0\u3002\u8be5\u6587\u7ae0\u603b\u7ed3\u4e86\u76ee\u524d\u5404\u79cd\u57fa\u56e0\u7ec4\u7f16\u8f91\u673a\u5668\u7684\u4f53\u5185<\/strong>\u4f20\u9012\u65b9\u6cd5\uff0c\u5e76\u5bf9\u76ee\u524d\u8be5\u9886\u57df\u9762\u4e34\u7684\u6311\u6218\u548c\u89e3\u51b3\u65b9\u6848\u63d0\u51fa\u4e86\u5c55\u671b\u3002<\/p>\n

\u3010\u56fe\u6587\u7b80\u4ecb\u3011<\/strong><\/p>\n

\u56fe<\/strong>1 CRISPR-Cas9<\/strong>\u4e0e\u57fa\u56e0\u7ec4\u7f16\u8f91\u673a\u5236<\/strong><\/p>\n

\"\"<\/p>\n

\u56fe<\/strong>2 CRISPR-Cas9<\/strong>\u7684\u9012\u9001\u548c\u8868\u8fbe\u65b9\u6cd5<\/strong><\/p>\n

\"\"<\/p>\n

\u56fe<\/strong>3 <\/strong>\u4ece\u4f53\u5185\u7cfb\u7edf\u5faa\u73af\u5230\u7ec6\u80de\u6838\u4f20\u9012\u7684\u751f\u7269\u5c4f\u969c<\/strong><\/p>\n

\"\"<\/p>\n

\u56fe<\/strong>4<\/strong>\u4ee5\u75c5\u6bd2\u4e3a\u8f7d\u4f53\u7684\u57fa\u56e0\u7ec4\u7f16\u8f91\u673a\u5668\u4f53\u5185\u9012\u9001<\/strong><\/p>\n

\"\"<\/p>\n

\u56fe<\/strong>5<\/strong>\u57fa\u4e8e\u5de5\u7a0b\u6750\u6599\u7684\u57fa\u56e0\u7ec4\u7f16\u8f91\u673a\u5668\u4f53\u5185\u9012\u9001\u5b9e\u4f8b<\/strong><\/p>\n

\"\"<\/p>\n

\u56fe<\/strong>6 <\/strong>\u5b9e\u73b0\u57fa\u56e0\u7ec4\u7f16\u8f91\u673a\u5668\u4f53\u5185\u9012\u9001\u9ad8\u6548\u7387\u3001\u9ad8\u7279\u5f02\u6027\u548c\u5b89\u5168\u6027\u7684\u7efc\u5408\u6027\u7b56\u7565<\/strong><\/p>\n

\"\"<\/p>\n

\u3010\u5c55\u671b\u3011<\/strong><\/p>\n

CRISPR-Cas9\u5df2\u7ecf\u5927\u5927\u6539\u53d8\u4e86\u57fa\u56e0\u7ec4\u7f16\u8f91\u9886\u57df\uff0c\u5e76\u6709\u671b\u5728\u751f\u7269\u533b\u5b66\u8bf8\u9886\u57df\u6380\u8d77\u4e00\u573a\u9769\u547d\u3002\u7136\u800c\uff0c\u57fa\u56e0\u7ec4\u7f16\u8f91\u673a\u5668\u7684\u4f53\u5185\u4f20\u9012\u4ecd\u7136\u662f\u4e00\u4e2a\u6311\u6218\uff0c\u9700\u8981\u65b0\u7684\u65b9\u6cd5\u6765\u5b9e\u73b0\u4f53\u5185\u57fa\u56e0\u7ec4\u7f16\u8f91\u7684\u9ad8\u4f20\u9012\u6548\u7387\u3001\u7ec4\u7ec7\u7279\u5f02\u6027\u548c\u5b89\u5168\u6027\u3002\u4e3a\u6b64\uff0c\u6211\u4eec\u53ef\u4ee5\u7efc\u5408\u8003\u8651\u4e00\u4e9b\u91cd\u8981\u7684\u8bbe\u8ba1\u7279\u6027\u3002\u4f8b\u5982\uff0c\u4e3a\u4e86\u5b9e\u73b0\u9ad8\u6548\u7387\u57fa\u56e0\u7ec4\u7f16\u8f91\uff0c\u4f20\u9012\u8f7d\u4f53\u9700\u8981\u5728\u7cfb\u7edf\u5faa\u73af\u671f\u95f4\u663e\u8457\u51cf\u5c11\u6216\u9632\u6b62CRISPR-Cas9\u7cfb\u7edf\u7684\u9176\u964d\u89e3\u3002\u8fd9\u53ef\u4ee5\u901a\u8fc7\u5305\u88c5\u8f7d\u4f53\u4ee5\u63d0\u9ad8\u5176\u5728\u8840\u6db2\u4e2d\u7684\u7a33\u5b9a\u6027\uff0c\u53ca\u5bf9gRNA\u548cDNA\u6a21\u677f\u8fdb\u884c\u5316\u5b66\u4fee\u9970\u6765\u5b9e\u73b0\u3002\u8f7d\u4f53\u5e94\u907f\u514d\u88ab\u541e\u566c\u7cfb\u7edf\u4ece\u4f53\u5185\u7684\u5feb\u901f\u6e05\u9664\u3002\u8fd9\u53ef\u4ee5\u901a\u8fc7\u5728\u7528\u751f\u7269\u76f8\u5bb9\u6027\u597d\u7684\u805a\u5408\u7269\uff08\u5982\u805a\u4e59\u4e8c\u9187\uff09\u6d82\u5c42\u548c\u4e0e\u80fd\u591f\u6d82\u5c42\u4e0a\u52a0\u88c5\u907f\u514d\u514d\u75ab\u53cd\u5e94\u9003\u7684\u914d\u4f53\u7ed3\u5408\u6765\u5b9e\u73b0\u3002\u8f7d\u4f53\u4e5f\u5e94\u8be5\u80fd\u591f\u4ece\u8840\u7ba1\u4e2d\u6e17\u51fa\u3002\u7814\u7a76\u8868\u660e\uff0c\u901a\u8fc7\u9776\u5411\u7ec4\u7ec7\u7279\u5f02\u6027\u914d\u4f53\uff0c\u53ef\u4ee5\u5728\u809d\u548c\u80bf\u7624\u4ee5\u5916\u7684\u7ec4\u7ec7\u4e2d\u5b9e\u73b0\u8f6c\u8fd0\u8f7d\u4f53\u7684\u5916\u6e17\u3002\u5728\u95f4\u8d28\u7a7a\u95f4\uff0c \u8f7d\u4f53\u9700\u8981\u6709\u6548\u5730\u8fc1\u79fb\u4ee5\u5230\u8fbe\u7ec4\u7ec7\u4e2d\u7684\u9776\u7ec6\u80de\u3002\u901a\u8fc7\u4f18\u5316\u7eb3\u7c73\u9897\u7c92\u7684\u5927\u5c0f\u548c\u6d82\u5c42\u548c\/\u6216\u901a\u8fc7\u9176\u964d\u89e3\u4fee\u9970\u7ec6\u80de\u5916\u57fa\u8d28\u53ef\u4ee5\u6539\u5584\u95f4\u8d28\u8f6c\u8fd0\u3002\u901a\u8fc7\u9776\u5411\u6fc0\u6d3b\u5185\u541e\u4f5c\u7528\u7684\u7ec6\u80de\u8868\u9762\u53d7\u4f53\uff0c\u53ef\u4ee5\u589e\u5f3a\u7eb3\u7c73\u7c92\u7684\u7ec6\u80de\u6444\u53d6\u3002\u5728\u7ec6\u80de\u5185\u541e\u8fc7\u7a0b\u4e2d\uff0c \u8f7d\u4f53\u9700\u8981\u901a\u8fc7\u7528\u5e26\u6b63\u7535\u8377\u7684\u805a\u5408\u7269\u7b49\u8bd5\u5242\u7834\u574f\u5185\u4f53\u819c\u7684\u7a33\u5b9a\u6027\uff0c\u4ee5\u5c06\u57fa\u56e0\u7ec4\u7f16\u8f91\u673a\u5668\u8d27\u7269\u91ca\u653e\u5230\u80de\u6d46\u4e2d\u3002 \u57fa\u4e8e\u9664\u4e86\u63d0\u4f9b\u57fa\u673a\u5236\u5916\uff0c\u63d0\u4f9b\u8bd5\u5242\u7684\u75c5\u6bd2\u6216\u975e\u75c5\u6bd2\u7684\u4f20\u9012\u7cfb\u7edf\u8fd8\u53ef\u4ee5\u63d0\u9ad8\u57fa\u56e0\u7ec4\u4e2d\u76ee\u6807\u57fa\u56e0\u5ea7\u7684\u53ef\u63a5\u8fd1\u6027\uff0c\u6216\u8d4b\u4e88\u7f16\u8f91\u7ec6\u80de\u9009\u62e9\u6027\u4f18\u52bf\uff08\u4f7f\u5176\u6bd4\u672a\u7f16\u8f91\u7ec6\u80de\u5b58\u6d3b\u548c\u751f\u957f\u66f4\u597d\uff09\uff0c\u4ece\u800c\u63d0\u9ad8\u603b\u4f53\u57fa\u56e0\u7f16\u8f91\u6548\u7387\u3002<\/p>\n

\u4e3a\u4e86\u63d0\u9ad8\u7ec4\u7ec7\u7279\u5f02\u6027\uff0c\u8f7d\u4f53\u53ef\u4ee5\u4e0e\u7ec6\u80de\u7279\u5f02\u6027\u9776\u5411\u914d\u4f53\u7ed3\u5408\u3002\u7ec4\u7ec7\u7279\u5f02\u6027\u4e5f\u53ef\u4ee5\u901a\u8fc7\u8bbe\u8ba1\u8fd0\u8f7d\u5de5\u5177\u6765\u5b9e\u73b0\uff0c\u8be5\u8fd0\u8f7d\u5de5\u5177\u53ef\u4ee5\u88ab\u5916\u90e8\u5149\u3001\u70ed\u6216\u78c1\u573a\u5c40\u90e8\u6fc0\u6d3b\uff0c\u6216\u8005\u5bf9\u7ec4\u7ec7\u7279\u5f02\u6027\u5fae\u73af\u5883\u4f5c\u51fa\u53cd\u5e94\uff0c\u4f8b\u5982PH\u503c\u548c\u9176\u6d3b\u6027\u3002\u4e3a\u4e86\u63d0\u9ad8\u5b89\u5168\u6027\uff0c\u53ef\u4ee5\u901a\u8fc7\u5c40\u90e8\u6fc0\u6d3b\u6765\u9632\u6b62CRISPR-Cas9\u7684\u7cfb\u7edf\u6027\u4f20\u9012\u3002\u6b64\u5916\uff0c\u81ea\u6211\u5220\u9664\u7684AAV\u7cfb\u7edf\u6216\u5177\u6709\u81ea\u6211\u5220\u9664\u529f\u80fd\u7684\u5176\u4ed6\u7c7b\u578b\u7684\u75c5\u6bd2\u8f7d\u4f53\u53ef\u7528\u6765\u51cf\u5c11\u8131\u9776\u53ca\u514d\u75ab\u53cd\u5e94\uff0c\u4ece\u800c\u63d0\u9ad8\u5b89\u5168\u6027\uff0c\u540c\u65f6\u4fdd\u6301\u5176\u9884\u5b9a\u7684\u7f16\u8f91\u529f\u80fd\u3002\u901a\u8fc7\u4f18\u5316\u5242\u91cf\u548c\/\u6216\u9650\u5236CAS9\u6d3b\u6027\u7684\u6301\u7eed\u65f6\u95f4\uff0c\u53ef\u4ee5\u51cf\u5c11\u6216\u6700\u5c0f\u5316CRISPR-Cas9\u7684\u975e\u9776\u5411\u6d3b\u6027\u3002\u9664\u4e86\u901a\u8fc7\u4f20\u9012Cas9 mRNA\u6216Cas9\u2013gRNA RNP\u6765\u90e8\u5206\u5b9e\u73b0\u5bf9Cas9\u6d3b\u6027\u7684\u65f6\u95f4\u63a7\u5236\uff0c\u4e5f\u53ef\u4ee5\u4f7f\u7528\u5176\u4ed6\u66f4\u7cbe\u51c6\u7684\u673a\u5236\uff0c\u4f8b\u5982\u4f7f\u7528\u5c0f\u5206\u5b50Cas9\u6291\u5236\u5242\u6216\u5149\u3002\u6b64\u5916\uff0c\u53ef\u7528\u751f\u7269\u76f8\u5bb9\u6027\u597d\u548c\u514d\u75ab\u53cd\u5e94\u4f4e\u7684\u6750\u6599\uff0c\u4ee5\u5c3d\u91cf\u51cf\u5c11\u5c40\u90e8\u708e\u75c7\u53cd\u5e94\u3002\u6700\u540e\uff0c\u9700\u8981\u6839\u636e\u6cbb\u7597\u9776\u70b9\u3001\u75be\u75c5\u72b6\u6001\u548c\u9776\u7ec4\u7ec7\u7279\u6027\u8bbe\u8ba1\u57fa\u56e0\u7ec4\u7f16\u8f91\u673a\u5668\u7684\u4f53\u5185\u9012\u9001\u3002<\/p>\n

\u00a0 \u4e3a\u4e86\u4f7f\u57fa\u4e8e\u5de5\u7a0b\u6750\u6599\u7684\u8f7d\u4f53\u5728\u4f53\u5185\u57fa\u56e0\u7ec4\u7f16\u8f91\u4e2d\u5b9e\u73b0\u9ad8\u6548\u3001\u7ec4\u7ec7\u7279\u5f02\u6027\u548c\u5b89\u5168\u6027\uff0c\u5efa\u6a21\u3001\u6a21\u62df\u548c\u8ba1\u7b97\u5206\u6790\u53ef\u5e2e\u52a9\u5176\u8bbe\u8ba1\u3001\u8bc4\u4f30\u548c\u4f18\u5316\u3002\u6b64\u5916\uff0c\u7cfb\u7edf\u5de5\u7a0b\u7684\u65b9\u6cd5\u53ef\u4ee5\u5e2e\u52a9\u6574\u5408\u8f7d\u4f53\u7684\u4e0d\u540c\u529f\u80fd\u3002\u4f8b\u5982\uff0c\u53ef\u4ee5\u8bbe\u8ba1\u4e00\u79cd\u53ef\u6d3b\u5316\u4e14\u751f\u7269\u76f8\u5bb9\u7684\u6d82\u5c42\uff0c\u4ee5\u4fdd\u62a4\u57fa\u56e0\u7ec4\u7f16\u8f91\u673a\u5668\uff0c\u51cf\u5c11\u86cb\u767d\u8d28\u5438\u9644\uff0c\u5e76\u6839\u636e\u5916\u90e8\u6216\u5185\u90e8\u4fe1\u53f7\u91ca\u653e\u57fa\u56e0\u7ec4\u7f16\u8f91\u673a\u5668\u3002\u591a\u4e2a\u914d\u4f53\u53ef\u4ee5\u7ed3\u5408\u5230\u7eb3\u7c73\u9897\u7c92\u8f7d\u4f53\u7684\u8868\u9762\uff0c\u4ee5\u540c\u65f6\u6539\u5584\u7ec4\u7ec7\u7279\u5f02\u6027\u9012\u9001\u3001\u8bf1\u5bfc\u7ec6\u80de\u8f6c\u67d3\u548c\u51cf\u5c11\u514d\u75ab\u7cfb\u7edf\u7684\u8bc6\u522b\u3002<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1a<\/strong>Engineered materials for in\u00a0vivo delivery of genome-editing machinery<\/a>, 2019, Nature Reviews Materials, DOI: 10.1038\/s41578-019-0145-9.<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Nature Reviews Materials \u7efc\u8ff0\uff1a\u57fa\u56e0\u7ec4\u7f16\u8f91\u673a\u5668\u4f53\u5185\u9012\u9001\u7684\u5de5\u7a0b\u6750\u6599 \u3010\u80cc\u666f\u4e0e\u7814\u7a76\u8fdb\u5c55\u3011 \u57fa\u56e0\u7ec4\u7f16\u8f91\u6280\u672f\uff0c\u5982CRISPR-CAS9\uff0c\u6709\u671b\u6cbb\u7597…<\/p>\n","protected":false},"author":2114,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[533],"tags":[],"class_list":["post-187583","post","type-post","status-publish","format-standard","hentry","category-zongshu"],"_links":{"self":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/187583"}],"collection":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/users\/2114"}],"replies":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=187583"}],"version-history":[{"count":3,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/187583\/revisions"}],"predecessor-version":[{"id":187869,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/187583\/revisions\/187869"}],"wp:attachment":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=187583"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=187583"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=187583"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}