{"id":184839,"date":"2019-09-02T06:30:27","date_gmt":"2019-09-01T22:30:27","guid":{"rendered":"\/\/m.iemloyee.com\/?p=184839"},"modified":"2019-09-01T20:38:12","modified_gmt":"2019-09-01T12:38:12","slug":"%e3%80%90%e5%ae%a1%e7%a8%bf%e5%b7%b2%e5%9b%9e%e5%a4%8d%e3%80%91%e6%96%b0%e5%8a%a0%e5%9d%a1%e5%9b%bd%e7%ab%8b%e5%a4%a7%e5%ad%a6%e5%88%98%e6%96%8c%e6%95%99%e6%8e%88angew-chem-int-ed-%e9%80%9a","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=184839","title":{"rendered":"\u65b0\u52a0\u5761\u56fd\u7acb\u5927\u5b66\u5218\u658c\u6559\u6388Angew. Chem. Int. Ed.:\u901a\u8fc7\u4ee3\u8c22\u6807\u8bb0\u5b9e\u73b0\u7ec6\u80de\u5185\u7ec6\u83cc\u75c5\u539f\u4f53\u53ef\u89c6\u5316\u548c\u539f\u4f4d\u6e05\u9664"},"content":{"rendered":"<p>\u3010<strong>\u5f15\u8a00<\/strong>\u3011<\/p>\n<p>\u5de8\u566c\u7ec6\u80de\u901a\u8fc7\u8bc6\u522b\u3001\u6444\u53d6\u548c\u6d88\u5316\u4fb5\u5165\u6027\u7ec6\u83cc\u75c5\u539f\u4f53\uff0c\u6210\u4e3a\u62b5\u5fa1\u7ec6\u83cc\u611f\u67d3\u7684\u7b2c\u4e00\u9053\u9632\u7ebf\u3002\u7136\u800c\uff0c\u7279\u5b9a\u7ec6\u80de\u5185\u7ec6\u83cc\u7684\u5206\u6ccc\u7cfb\u7edf\u53ef\u4ee5\u4f7f\u5de8\u566c\u7ec6\u80de\u4e2d\u75c5\u539f\u4f53\u6301\u7eed\u5b58\u6d3b\u3002\u5728\u5bbf\u4e3b\u5de8\u566c\u7ec6\u80de\u4e2d\u5b58\u6d3b\uff0c\u7ec6\u80de\u5185\u7ec6\u83cc\u53ef\u514d\u53d7\u5176\u4ed6\u514d\u75ab\u653b\u51fb\u3002\u5728\u8fd9\u79cd\u60c5\u51b5\u4e0b\uff0c\u5de8\u566c\u7ec6\u80de\u4e0d\u4ec5\u4e0d\u80fd\u6d88\u9664\u7ec6\u83cc\uff0c\u4e5f\u53ef\u80fd\u6210\u4e3a\u7ec6\u83cc\u7e41\u6b96\u7684\u6765\u6e90\uff0c\u8fd9\u53ef\u80fd\u5bfc\u81f4\u4e25\u91cd\u7684\u4f20\u67d3\u75c5\uff0c\u5982\u7ed3\u6838\u75c5\u3002\u6b64\u5916\uff0c\u5bbf\u4e3b\u5de8\u566c\u7ec6\u80de\u7684\u5c4f\u853d\u4f5c\u7528\u663e\u8457\u963b\u788d\u4e86\u7ec6\u80de\u5185\u7ec6\u83cc\u7684\u68c0\u6d4b\u548c\u6d88\u878d\u3002\u8fd9\u589e\u52a0\u4e86\u7ec6\u80de\u5185\u7ec6\u83cc\u7684\u884c\u4e3a\u7814\u7a76\u96be\u5ea6\uff0c\u5e76\u964d\u4f4e\u4e86\u6297\u751f\u7d20\u5bf9\u7ec6\u80de\u5185\u7ec6\u83cc\u76f8\u5173\u75be\u75c5\u7684\u6cbb\u7597\u6548\u7387\u3002<\/p>\n<p>\u57fa\u4e8e\u5bf9\u7ec6\u83cc\u80bd\u805a\u7cd6\u7684\u6210\u50cf\uff0c\u751f\u7269\u6b63\u4ea4\u6807\u8bb0\u5df2\u6210\u4e3a\u7528\u4e8e\u68c0\u6d4b\u7ec6\u83cc\u7684\u6709\u6548\u7b56\u7565\u3002\u80bd\u805a\u7cd6\u662f\u91cd\u590d\u5355\u5143\u4e3aN-\u4e59\u9170\u8461\u7cd6\u80fa\uff08GlcNAc\uff09\u548cN-\u4e59\u9170\u80de\u58c1\u9178\uff08MurNAc\uff09\u7684\u4ea4\u8054\u7f51\u72b6\u4f53\u3002\u5728\u7ec6\u83cc\u4e0e\u4ee3\u8c22\u524d\u4f53\u6e29\u80b2\u540e\uff0c\u7ec6\u83cc\u7ec6\u80de\u58c1\u4e0a\u8868\u8fbe\u53cd\u5e94\u6027\u5316\u5b66\u57fa\u56e2\uff08\u4f8b\u5982\u53e0\u6c2e\u5316\u7269\uff0c\u7094\u70c3\uff09\u3002\u968f\u540e\u5f15\u5165\u76f8\u5e94\u7684\u751f\u7269\u6b63\u4ea4\u57fa\u56e2\u4fee\u9970\u7684\u8367\u5149\u56e2\u53ef\u4ee5\u901a\u8fc7\u8fde\u63a5\u53cd\u5e94\u6709\u6548\u5730\u6807\u8bb0\u7ec6\u83cc\u80bd\u805a\u7cd6\u3002\u7531\u4e8e\u5bbf\u4e3b\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u4ec5\u4f7f\u7528\u4e00\u7ec4\u786e\u5b9a\u7684L-\u6c28\u57fa\u9178\u8fdb\u884c\u751f\u7269\u5408\u6210\uff0c\u56e0\u6b64\u5f15\u5165\u7684D-\u6c28\u57fa\u9178\u53ef\u4ee5\u7279\u5f02\u6027\u5730\u6807\u8bb0\u7ec6\u83cc\u7684\u80bd\u805a\u7cd6\uff0c\u800c\u4e0d\u80fd\u6807\u8bb0\u5bbf\u4e3b\u7ec6\u80de\u3002\u56e0\u6b64\uff0c\u8be5\u7b56\u7565\u6709\u671b\u8ddf\u8e2a\u5bbf\u4e3b\u7ec6\u80de\u4e2d\u7684\u7ec6\u83cc\u3002\u4e0d\u5e78\u7684\u662f\uff0c\u7531\u4e8e\u76ee\u524d\u7684\u751f\u7269\u6b63\u4ea4\u53cd\u5e94\u901a\u5e38\u9700\u8981\u4e24\u6b65\u64cd\u4f5c\uff0c\u751a\u81f3\u53ef\u80fd\u9700\u8981\u590d\u5408\u50ac\u5316\u5242\u8fdb\u884c\u8fde\u63a5\u53cd\u5e94\uff0c\u5230\u76ee\u524d\u4e3a\u6b62\uff0c\u7ec6\u80de\u5185\u7ec6\u83cc\u7684\u6807\u8bb0\u8fc7\u7a0b\u9700\u8981\u56fa\u5b9a\u611f\u67d3\u6027\u5bbf\u4e3b\u7ec6\u80de\u6216\u7528\u5bbf\u4e3b\u7ec6\u80de\u5916\u7684\u4ee3\u8c22\u524d\u4f53\u9884\u5904\u7406\u7ec6\u83cc\u3002\u76ee\u524d\u6ca1\u6709\u53ef\u4ee5\u5728\u6d3b\u7ec6\u80de\u4e2d\u5b9e\u73b0\u7ec6\u80de\u5185\u7ec6\u83cc\u7684\u5b9e\u65f6\u8ffd\u8e2a\u7684\u62a5\u9053\uff0c\u66f4\u4e0d\u7528\u8bf4\u5bf9\u7ec6\u83cc\u8fdb\u884c\u539f\u4f4d\u6d88\u878d\u3002<\/p>\n<p>\u3010<strong>\u6210\u679c\u7b80\u4ecb<\/strong>\u3011<\/p>\n<p>\u8fd1\u65e5\uff0c<strong>\u65b0\u52a0\u5761\u56fd\u7acb\u5927\u5b66\u5218\u658c\u6559\u6388\u8bfe\u9898\u7ec4<\/strong>\u6210\u529f\u5f00\u53d1\u4e86\u4e00\u79cd\u7ec6\u83cc\u53ef\u4ee3\u8c22\u7684\u53cc\u529f\u80fd\u63a2\u9488TPEPy-D-Ala\uff0c\u5b83\u57fa\u4e8eD-\u4e19\u6c28\u9178\u548c\u4e00\u79cd\u5177\u6709\u805a\u96c6\u8bf1\u5bfc\u53d1\u5149\u7279\u6027\u7684\u5149\u654f\u5242\uff0c\u7528\u4e8e\u6d3b\u4f53\u5bbf\u4e3b\u7ec6\u80de\u4e2d\u7ec6\u80de\u5185\u7ec6\u83cc\u7684\u8367\u5149\u70b9\u4eae\u6210\u50cf\u548c\u5149\u52a8\u529b\u5b66\u6d88\u878d\u3002\u4e00\u65e6\u4ee3\u8c22\u7ed3\u5408\u5230\u7ec6\u83cc\u80bd\u805a\u7cd6\u4e2d\uff0cTPEPy-D-Ala\u7684\u5206\u5b50\u5185\u8fd0\u52a8\u88ab\u6291\u5236\u4ee5\u589e\u5f3a\u8367\u5149\u4fe1\u53f7\uff0c\u4ece\u800c\u63d0\u4f9b\u7ec6\u80de\u5185\u7ec6\u83cc\u7684\u6e05\u6670\u6210\u50cf\u3002\u6b64\u5916\uff0cTPEPy-D-Ala\u53ef\u4ee5\u901a\u8fc7\u4e0e\u80bd\u805a\u7cd6\u7684\u5171\u4ef7\u8fde\u63a5\u539f\u4f4d\u6709\u6548\u5730\u6d88\u878d\u6807\u8bb0\u7ec6\u80de\u5185\u7684\u7ec6\u83cc\uff0c\u4ea7\u751f20\u00b10.5\u03bcg\/mL\u7684\u4f4e\u7ec6\u80de\u5185\u6700\u5c0f\u6291\u5236\u6d53\u5ea6\uff08MIC\uff09\uff0c\u6bd4\u901a\u5e38\u4f7f\u7528\u6297\u751f\u7d20\u3001\u4e07\u53e4\u9709\u7d20\u66f4\u52a0\u6709\u6548\u3002<strong>\u8be5\u6210\u679c\u4ee5\u9898\u4e3a\u201cVisualization and in-situ Ablation of Intracellular Bacterial Pathogen through Metabolic Labeling\u201d\u53d1\u8868\u5728Angew. Chem. Int. Ed.\u4e0a\u3002<\/strong><\/p>\n<p><strong>\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/strong><\/p>\n<p><strong>Figure 1.<\/strong><strong>\u63a2\u9488\u5206\u5b50\u7684\u5149\u7269\u7406\u6027\u8d28\u8868\u5f81<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-184840\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2019\/09\/r1.jpg\" alt=\"\" width=\"600\" height=\"587\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2019\/09\/r1.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2019\/09\/r1-300x294.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>\uff08a\uff09TPEPy-D-Ala\u548cTPEPy-L-Ala\u7684\u5316\u5b66\u7ed3\u6784\u548c\u5408\u6210\u9014\u5f84<\/p>\n<p>\uff08b\uff09TPEPy-D-Ala\u548cTPEPy-\u4e01\u7094\u5728PBS\u4e2d\u7684\u5438\u6536\u548c\u8367\u5149\u5149\u8c31<\/p>\n<p>\uff08c\uff09TPEPy-D-Ala\u5728\u4e0d\u540cTHF \/\u919a\u6df7\u5408\u7269\u4e2d\u7684\u8367\u5149\u5149\u8c31<\/p>\n<p>\uff08d\uff09\u5728PBS\u4e2d\u6216\u5728\u4e0eBSA\uff080.01mg\/mL\uff09\uff0c\u78f7\u8102\uff080.01mg\/mL\uff09\uff0c\u5de8\u566c\u7ec6\u80de\u88c2\u89e3\u7269\u548cMRSA\u5b75\u80b2\u540e\u5728650nm\u5904TPEPy-D-Ala\u7684\u76f8\u5bf9\u8367\u5149\u5f3a\u5ea6;<\/p>\n<p>\uff08e\uff09\u5728\u4e0d\u540c\u7684\u767d\u5149\u7167\u5c04\uff0860mW\/cm<sup>2<\/sup>\uff09\u65f6\u95f4\u4e0bABDA\u548cTPEPy-D-Ala\u6df7\u5408\u7269\u7684\u5438\u6536\u5149\u8c31<\/p>\n<p><strong>Figure 2.TPEPy-D-Ala<\/strong><strong>\u5728\u80bd\u805a\u7cd6\u751f\u7269\u5408\u6210\u4e2d\u7684\u751f\u7269\u6210\u50cf<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-184841\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2019\/09\/r2.jpg\" alt=\"\" width=\"600\" height=\"651\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2019\/09\/r2.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2019\/09\/r2-276x300.jpg 276w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>\uff08a\uff09\u63a2\u9488\u5206\u5b50\u7684\u6210\u50cf\u673a\u7406\u793a\u610f\u56fe<\/p>\n<p>\uff08b\uff09\u63a2\u9488\u5206\u5b50\u5bf9MRSA\u7684\u8367\u5149\u6210\u50cf<\/p>\n<p>\uff08c\uff09\u63a2\u9488\u5206\u5b50\u5bf9\u5927\u80a0\u6746\u83cc\u7684\u8367\u5149\u6210\u50cf<\/p>\n<p><strong>Figure 3.<\/strong><strong>MRSA<\/strong><strong>\u611f\u67d3\u7684<\/strong><strong>RAW 264.7<\/strong><strong>\u7ec6\u80de\u7684\u6210\u50cf<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-184842\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2019\/09\/r3.jpg\" alt=\"\" width=\"600\" height=\"445\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2019\/09\/r3.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2019\/09\/r3-300x223.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>\u7528TPEPy-D-Ala\uff08a\uff09\u6216TPEPy-L-Ala\u4e0eHoechst 33342\uff08b\uff09\u5904\u7406\u7684MRSA\u611f\u67d3\u7684RAW 264.7\u7ec6\u80de\u7684\u6210\u50cf<\/p>\n<p><strong>Figure 4.<\/strong><strong>\u5149\u52a8\u529b\u5b66\u6cbb\u7597<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-184843\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2019\/09\/r4.jpg\" alt=\"\" width=\"600\" height=\"245\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2019\/09\/r4.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2019\/09\/r4-300x123.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>\uff08a\uff09\u5728\u5149\u7167\u5c04\u4e0b\u7528\u4e0d\u540c\u6d53\u5ea6\u7684TPEPy-D-Ala\u5904\u7406\u7ec6\u80de\u5185MRSA\u7684CFU<\/p>\n<p>\uff08b\uff09\u5728\u9ed1\u6697\u6761\u4ef6\u4e0b\u6216\u5149\u7167\u5c04\u4e0b\u7528\u4e0d\u540c\u6d53\u5ea6\u7684TPEPy-D-Ala\u5904\u7406\u7684RAW 264.7\u7ec6\u80de\u7684\u7ec6\u80de\u6d3b\u529b<\/p>\n<p><strong>\u3010\u5c0f\u7ed3\u3011<\/strong><\/p>\n<p>\u5728\u8fd9\u4e2a\u5de5\u4f5c\u4e2d\uff0c\u4f5c\u8005\u5f00\u53d1\u4e86\u4e00\u79cd\u5b9e\u65f6\u7ec6\u80de\u5185\u7ec6\u83cc\u6807\u8bb0\u7684\u70b9\u4eae\u63a2\u9488\u548c\u539f\u4f4d\u5149\u52a8\u529b\u5b66\u6d88\u878d\u3002\u4e00\u65e6\u4ee3\u8c22\u7ed3\u5408\u5230\u80bd\u805a\u7cd6\u4e2d\uff0c\u63a2\u9488\u53ef\u4ee5\u6e05\u695a\u5730\u8ffd\u8e2a\u9690\u85cf\u5728\u6d3b\u5de8\u566c\u7ec6\u80de\u4e2d\u7684\u7ec6\u80de\u5185\u7ec6\u83cc\u3002\u5728\u5b9a\u4f4d\u8fd9\u4e9b\u7ec6\u80de\u5185\u7ec6\u83cc\u540e\uff0c\u63a2\u9488\u53ef\u4ee5\u5728\u5149\u7167\u5c04\u4e0b\u6709\u6548\u5730\u6d88\u878d\u5b83\u4eec\u3002\u8fd9\u4e2a\u5de5\u4f5c\u9996\u6b21\u5b9e\u73b0\u4e86\u6d3b\u7ec6\u80de\u4e2d\u7ec6\u80de\u5185\u7ec6\u83cc\u7684\u53ef\u89c6\u5316\u548c\u6d88\u9664\u3002\u4f5c\u8005\u6240\u5f00\u53d1\u7684\u7b56\u7565\u5c06\u6709\u76ca\u4e8e\u7ec6\u80de\u5185\u7ec6\u83cc\u76f8\u5173\u75be\u75c5\u7684\u5b9e\u65f6\u68c0\u6d4b\u548c\u6cbb\u7597\u3002<\/p>\n<p><a href=\"https:\/\/onlinelibrary.wiley.com\/doi\/abs\/10.1002\/anie.201910187\" target=\"_blank\" rel=\"noopener\">Visualization and in-situ Ablation of Intracellular Bacterial Pathogen through Metabolic Labeling<\/a><\/p>\n<p>(Angew. Chem. Int. 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