{"id":183740,"date":"2019-08-20T09:43:53","date_gmt":"2019-08-20T01:43:53","guid":{"rendered":"\/\/m.iemloyee.com\/?p=183740"},"modified":"2019-08-20T09:44:09","modified_gmt":"2019-08-20T01:44:09","slug":"%e3%80%90%e5%ae%a1%e7%a8%bf%e6%9c%aa%e5%9b%9e%e5%a4%8d%e3%80%91%e5%8d%8e%e4%b8%9c%e5%b8%88%e8%8c%83%e5%a4%a7%e5%ad%a6%e8%a3%b4%e6%b5%a9%e6%95%99%e6%8e%88angew-chem-int-ed-%e7%94%a8%e4%ba%8e","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=183740","title":{"rendered":"\u534e\u4e1c\u5e08\u8303\u5927\u5b66\u88f4\u6d69\u6559\u6388Angew. Chem. Int. Ed.:\u7528\u4e8e\u7ec6\u83cc\u8868\u578b\u9274\u5b9a\u7684DNA\u5206\u5b50\u63a2\u9488"},"content":{"rendered":"<p>\u3010<strong>\u5f15\u8a00<\/strong>\u3011<\/p>\n<p>\u8d85\u7075\u654f\u68c0\u6d4b\u548c\u75c5\u539f\u4f53\u7684\u5feb\u901f\u9274\u5b9a\u5bf9\u4e8e\u901a\u8fc7\u7528\u9002\u5f53\u7684\u6297\u751f\u7d20\u6cbb\u7597\u6765\u6539\u5584\u60a3\u6709\u7ec6\u83cc\u611f\u67d3\u7684\u60a3\u8005\u7684\u5b58\u6d3b\u7387\u662f\u81f3\u5173\u91cd\u8981\u7684\u3002\u76ee\u524d\u7ec6\u83cc\u8840\u6db2\u57f9\u517b\u7684\u4e34\u5e8a\u6807\u51c6\u9700\u8981\u5927\u91cf\u7684\u8840\u6db2\u6837\u672c\u548c\u7e41\u7410\u7684\u57f9\u517b\u548c\u5fae\u751f\u7269\u8868\u578b\u9274\u5b9a\uff0c\u5e76\u4e14\u6574\u4e2a\u9274\u5b9a\u8fc7\u7a0b\u975e\u5e38\u8017\u65f6\uff08\u51e0\u5929\u751a\u81f3\u51e0\u5468\uff09\u5e76\u4e14\u901a\u5e38\u53d6\u51b3\u4e8e\u64cd\u4f5c\u8005\u7684\u4e13\u4e1a\u77e5\u8bc6\u3002\u4f5c\u4e3a\u66ff\u4ee3\u65b9\u6848\uff0c\u4e00\u4e9b\u57fa\u56e0\u578b\u65b9\u6cd5\uff0c\u4f8b\u5982\u5b9e\u65f6\u805a\u5408\u9176\u94fe\u53cd\u5e94\uff08PCR\uff09\u548c\u8367\u5149\u539f\u4f4d\u6742\u4ea4\uff08FISH\uff09\u53ef\u4ee5\u6709\u6548\u5730\u5c06\u6d4b\u5b9a\u65f6\u95f4\u51cf\u5c11\u81f3\u8840\u57f9\u517b\u9633\u6027\u540e\u6570\u5c0f\u65f6\uff08\u7ea66-10\u5c0f\u65f6\uff09\u3002\u7136\u800c\uff0c\u6709\u9650\u7684\u68c0\u6d4b\u7075\u654f\u5ea6\u548c\u7279\u5f02\u6027\u4ee5\u53ca\u7531\u5927\u91cf\u5468\u56f4\u975e\u76ee\u6807\u7269\u79cd\u4ea7\u751f\u7684\u9ad8\u80cc\u666f\u4fe1\u53f7\u4ecd\u7136\u963b\u788d\u5b83\u4eec\u7684\u5e7f\u6cdb\u4f7f\u7528\u3002\u7136\u800c\uff0c\u6240\u6709\u8fd9\u4e9b\u5e38\u89c4\u65b9\u6cd5\u5728\u4e00\u5b9a\u7a0b\u5ea6\u4e0a\u6d89\u53ca\u590d\u6742\u548c\u8d39\u529b\u7684\u6837\u54c1\u5904\u7406\uff08\u4f8b\u5982\u7ec6\u80de\u57f9\u517b\uff0c\u7ec6\u80de\u88c2\u89e3\uff0c\u6838\u9178\u63d0\u53d6\u548cPCR\u6269\u589e\uff09\uff0c\u4f7f\u5f97\u4e34\u5e8a\u6837\u54c1\u7684\u9ad8\u901a\u91cf\u5206\u6790\u96be\u4ee5\u8fdb\u884c\u3002\u53e6\u5916\uff0c\u591a\u79cd\u7ec6\u83cc\u75c5\u539f\u4f53\u53ef\u4ee5\u5171\u5b58\uff0c\u4f46\u901a\u5e38\u5728\u8840\u6db2\u4e2d\u4ee5\u4f4e\u6c34\u5e73\u5b58\u5728\uff08\u4f8b\u5982\uff0c&lt;1-100CFU\/mL\uff09\u3002\u56e0\u6b64\uff0c\u975e\u5e38\u9700\u8981\u5f00\u53d1\u7528\u4e8e\u4e34\u5e8a\u8bca\u65ad\u4e2d\u75c5\u539f\u83cc\u7684\u9ad8\u901a\u91cf\u9274\u522b\u7684\u5feb\u901f\uff0c\u591a\u91cd\u548c\u8d85\u7075\u654f\u65b9\u6cd5\u3002<\/p>\n<p>\u57fa\u4e8e\u6db2\u6ef4\u7684\u5fae\u6d41\u4f53\u6280\u672f\u4e3a\u5355\u7ec6\u80de\u6c34\u5e73\u7684\u9ad8\u7075\u654f\u5ea6\u751f\u7269\u68c0\u6d4b\u548c\u5206\u6790\u63d0\u4f9b\u4e86\u4e00\u4e2a\u5f88\u6709\u524d\u9014\u7684\u5e73\u53f0\uff0c\u4f8b\u5982\u57fa\u56e0\u7ec4\u5206\u6790\uff0c\u5206\u6ccc\u8868\u578b\u7b5b\u9009\u548c\u591a\u91cd\u67d3\u8272\u8d28\u76f8\u4e92\u4f5c\u7528\u5206\u6790\u3002\u9274\u4e8e\u5176\u80fd\u591f\u901a\u8fc7\u7269\u7406\u548c\u5316\u5b66\u9694\u79bb\u5c06\u5c11\u91cf\u7ec6\u80de\u9650\u5236\u5728\u79bb\u6563\u6db2\u6ef4\u4e2d\uff0c\u8fd9\u79cd\u65b9\u6cd5\u4e5f\u9002\u7528\u4e8e\u7ec6\u83cc\u68c0\u6d4b\uff0c\u53ef\u4ee5\u76f4\u63a5\u4ece\u7a00\u91ca\u8840\u6db2\u4e2d\u5feb\u901f\u68c0\u6d4b\u7ec6\u83cc\uff0c\u5177\u6709\u5355\u7ec6\u83cc\u654f\u611f\u6027\u3002\u7136\u800c\uff0c\u7531\u4e8e\u7f3a\u4e4f\u6709\u6548\u7684\u4fe1\u53f7\u653e\u5927\uff0c\u8be5\u65b9\u6cd5\u4e0d\u5141\u8bb8\u68c0\u6d4b\u75c5\u539f\u4f53\u7684\u4f4e\u4e30\u5ea6\u751f\u7269\u6807\u8bb0\u7269\u3002\u6b64\u5916\uff0c\u5bf9\u4e8e\u591a\u79cd\u75c5\u539f\u4f53\u548c\u6613\u611f\u6027\u6a21\u5f0f\u7684\u540c\u65f6\u9274\u5b9a\u548c\u591a\u91cd\u68c0\u6d4b\u4ecd\u7136\u662f\u4e00\u4e2a\u5de8\u5927\u7684\u6311\u6218\u3002\u867d\u7136\u6807\u8bb0\u5177\u6709\u4e0d\u540c\u8367\u5149\u989c\u8272\u7684\u591a\u4e2a\u4f20\u611f\u5668\u5c06\u5141\u8bb8\u540c\u65f6\u591a\u8def\u68c0\u6d4b\u591a\u79cd\u75c5\u539f\u4f53\uff0c\u4f46\u662f\u5b83\u4eec\u7684\u591a\u8def\u590d\u7528\u80fd\u529b\u901a\u5e38\u53d7\u5230\u53ef\u5728\u6709\u6548\u8303\u56f4\u5185\u660e\u786e\u68c0\u6d4b\u5230\u7684\u6709\u9650\u6570\u91cf\u7684\u5149\u8c31\u989c\u8272\u7684\u9650\u5236\u3002\u56e0\u6b64\uff0c\u5f00\u53d1\u7528\u4e8e\u57fa\u4e8e\u6db2\u6ef4\u7684\u75c5\u539f\u4f53\u68c0\u6d4b\u7684\u8d85\u591a\u91cd\u7b56\u7565\u662f\u975e\u5e38\u9700\u8981\u7684\u3002<\/p>\n<p>\u3010<strong>\u6210\u679c\u7b80\u4ecb<\/strong>\u3011<\/p>\n<p>\u8fd1\u65e5\uff0c<strong>\u534e\u4e1c\u5e08\u8303\u5927\u5b66\u88f4\u6d69\u6559\u6388\u8bfe\u9898\u7ec4<\/strong>\u63d0\u51fa\u4e86\u4e00\u79cd\u5e73\u53f0\u6280\u672f\uff0c\u53ef\u7528\u4e8e\u8d85\u9ad8\u901a\u91cf\u7ec6\u83cc\u68c0\u6d4b\u7684\u4e00\u6b65\u6cd5\uff0c\u5feb\u901f\u548c\u8d85\u591a\u91cd\u65b9\u6cd5\u3002SDwalkers\u5141\u8bb8\u5c06\u5177\u6709\u8367\u5149\u5f3a\u5ea6\u548c\u53d1\u5c04\u6ce2\u957f\u7684\u6db2\u6ef4\u7f16\u7801\u7528\u4e8e\u540c\u65f6\u8bc6\u522b\u548c\u591a\u91cd\u68c0\u6d4b\u75c5\u539f\u4f53\u7684\u4e8c\u7ef4\uff082D\uff09\u6761\u5f62\u7801\u3002\u8be5\u7b56\u7565\u5c06\u6781\u5927\u5730\u6269\u5c55\u57fa\u4e8e\u6db2\u6ef4\u5fae\u6d41\u4f53\u7684\u591a\u8def\u590d\u7528\u80fd\u529b\uff0c\u7528\u4e8e\u7ec6\u83cc\u68c0\u6d4b\u5e76\u6709\u76ca\u4e8e\u5176\u4ed6\u751f\u7269\u6d4b\u5b9a\u5e94\u7528\u3002\u6b64\u5916\uff0cSDwalkers\u7684\u9176\u9a71\u52a8\u7ea7\u8054\u53cd\u5e94\u63d0\u4f9b\u4e86\u517c\u5bb9\u7684\u4fe1\u53f7\u653e\u5927\u7a0b\u5e8f\uff0c\u5927\u5927\u63d0\u9ad8\u4e86\u68c0\u6d4b\u6d3b\u7ec6\u83cc\u7684\u7075\u654f\u5ea6\uff0c\u540c\u65f6\u5177\u6709\u5355\u7ec6\u83cc\u9002\u7528\u6027\uff0c\u800c\u4e0d\u4f1a\u727a\u7272\u591a\u91cd\u4fe1\u53f7\u653e\u5927\u7684\u8d28\u91cf\u3002\u503c\u5f97\u6ce8\u610f\u7684\u662f\uff0c\u4f5c\u8005\u6210\u529f\u5730\u5c06\u8fd9\u79cdSDwalker-Drop\u7b56\u7565\u4e0e\u5546\u4e1a\u6d41\u5f0f\u7ec6\u80de\u4eea\uff08FCM\uff09\u6574\u5408\u7528\u4e8e\u4e34\u5e8a\u6837\u54c1\u5206\u6790\uff0c\u5176\u7ed3\u5408\u4e86\u6db2\u6ef4\u5fae\u6d41\u4f53\u7528\u4e8e\u5feb\u901f\u68c0\u6d4b\u7684\u76ca\u5904\uff0c\u7528\u4e8e\u8d85\u591a\u91cd\u6db2\u6ef4\u7f16\u7801\u548c\u8d85\u7075\u654f\u751f\u7269\u6d4b\u5b9a\u7684SDwalkers\u4ee5\u53ca\u7528\u4e8e\u591a\u53c2\u6570FCM\u7684\u591a\u53c2\u6570FCM\u8d85\u9ad8\u901a\u91cf\u7b5b\u9009\u3002\u603b\u800c\u8a00\u4e4b\uff0cSDwalker-Drop\u5e73\u53f0\u4e3a\u591a\u91cd\u5206\u5b50\u68c0\u6d4b\u63d0\u4f9b\u4e86\u5f3a\u5927\u7684\u5de5\u5177\uff0c\u6709\u5229\u4e8e\u4e34\u5e8a\u8bca\u65ad\uff0c\u73af\u5883\u5206\u6790\u548c\u7cbe\u51c6\u533b\u5b66\u3002<strong>\u8be5\u6210\u679c\u4ee5\u9898\u4e3a\u201cStochastic DNA Walker in Droplets for Super-Multiplex Bacteria Phenotype Detection\u201d\u53d1\u8868\u5728Angew. Chem. Int. Ed.\u4e0a\u3002<\/strong><\/p>\n<p><strong>\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/strong><\/p>\n<p><strong>Figure 1.<\/strong><strong>\u7528\u4e8e\u8d85\u590d\u5408\u7ec6\u83cc\u8868\u578b\u7684<\/strong><strong>SDwalker-Drop<\/strong><strong>\u5e73\u53f0\u7684\u793a\u610f\u56fe<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-183741\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2019\/08\/revised1-2.jpg\" alt=\"\" width=\"600\" height=\"210\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2019\/08\/revised1-2.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2019\/08\/revised1-2-300x105.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p><strong>Figure 2.<\/strong><strong>\u57fa\u4e8e<\/strong><strong>SDwalker<\/strong><strong>\u7684\u4f20\u611f\u5668\u7528\u4e8e\u7ec6\u83cc\u68c0\u6d4b<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-183742\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2019\/08\/revised2-2.jpg\" alt=\"\" width=\"600\" height=\"534\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2019\/08\/revised2-2.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2019\/08\/revised2-2-300x267.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>\uff08a\uff09SDwalker\u4f20\u611f\u5668\u7684\u5de5\u4f5c\u539f\u7406<\/p>\n<p>\uff08b\uff09\u901a\u8fc7TIRF\u5b9e\u65f6\u76d1\u6d4b\u5355\u4e2aSDwalker\u4f20\u611f\u5668<\/p>\n<p>\uff08c\uff09SDwalker\u5728\u4e0d\u540c\u6d53\u5ea6\u7684MRSA\u5b58\u5728\u4e0b\u7684\u8367\u5149\u54cd\u5e94<\/p>\n<p>\uff08d\uff09SDwalker\u5bf9\u4e0d\u540c\u7ec6\u83cc\u7684\u8367\u5149\u54cd\u5e94<\/p>\n<p><strong>Figure 3.<\/strong><strong>\u4f7f\u7528\u57fa\u4e8e<\/strong><strong>SDwalker-Drop<\/strong><strong>\u7684\u989c\u8272\u6216\u5f3a\u5ea6\u7f16\u7801\u6761\u5f62\u7801\u5bf9\u7ec6\u83cc\u8fdb\u884c\u591a\u91cd\u5206\u6790<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-183743\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2019\/08\/revised3-2.jpg\" alt=\"\" width=\"600\" height=\"789\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2019\/08\/revised3-2.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2019\/08\/revised3-2-228x300.jpg 228w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>\uff08a\uff09\u5206\u522b\u8bb0\u5f55\u5728\u84dd\u8272\uff0c\u7eff\u8272\u548c\u7ea2\u8272\u901a\u9053\u4e2d\u7684\u989c\u8272\u7f16\u7801\u6761\u5f62\u7801\u7684\u8367\u5149\u5149\u8c31\u548c\u56fe\u50cf<\/p>\n<p>\uff08b\uff09\u7528\u4e8e\u7ec6\u83cc\u7684\u8d85\u591a\u91cd\u5206\u6790\u7684\u5f3a\u5ea6\u7f16\u7801\u6761\u5f62\u7801\u7684\u793a\u610f\u56fe<\/p>\n<p>\uff08c\uff09\u901a\u8fc7\u5f3a\u5ea6\u7f16\u7801\u6761\u5f62\u7801\u53ef\u89c6\u5316\u5206\u6790\u7ed3\u679c\uff0c\u4ee5\u9274\u5b9a\u542b\u6709\u4e0d\u540c\u7ec6\u83cc\u7c7b\u578b\u7684\u6df7\u5408\u7269<\/p>\n<p>\uff08d\uff09\u6837\u54c1S1-S7\u7684\u8367\u5149\u5f3a\u5ea6<\/p>\n<p><strong>Figure 4.<\/strong> <strong>\u4f7f\u7528\u57fa\u4e8e<\/strong><strong>SDwalker-Drop<\/strong><strong>\u7684\u989c\u8272\u548c\u5f3a\u5ea6\u7f16\u7801\u6761\u5f62\u7801\u8fdb\u884c\u5b9e\u9645\u6837\u54c1\u5206\u6790<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-183744\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2019\/08\/revised4-2.jpg\" alt=\"\" width=\"600\" height=\"413\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2019\/08\/revised4-2.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2019\/08\/revised4-2-300x207.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>\uff08a\uff09\u7528\u4e8e\u5b9e\u9645\u6837\u672c\u5206\u6790\u7684SDwalker-Drop\u65b9\u6848<\/p>\n<p>\uff08b\uff09\u516d\u4e2a\u5b9e\u9645\u6837\u54c1\u7684\u8367\u5149\u5f3a\u5ea6\u7684\u6563\u70b9\u56fe<\/p>\n<p><strong>\u3010\u5c0f\u7ed3\u3011<\/strong><\/p>\n<p>\u5728\u8fd9\u4e2a\u5de5\u4f5c\u4e2d\uff0c\u4f5c\u8005\u5f00\u53d1\u4e86\u4e00\u79cd\u57fa\u4e8eSDwalker-Drops\u7684\u8d85\u7ea7\u591a\u8def\u590d\u7528\u7f16\u7801\u6280\u672f\u3002\u8fd9\u79cd\u7528\u4e8e\u7ec6\u83cc\u5206\u6790\u7684\u65b9\u6cd5\u5177\u6709\u72ec\u7279\u7684\u4f18\u70b9\uff1a\uff08i\uff09\u7531\u4e8e\u9176\u4fe1\u53f7\u653e\u5927\uff0c\u9176\u9a71\u52a8\u7684SDwalker\u80fd\u591f\u4ee5\u9ad8\u7075\u654f\u5ea6\u8fdb\u884c\u7ec6\u83cc\u68c0\u6d4b\u3002\uff08ii\uff09\u8fd9\u79cd\u4e0e\u5fae\u6d41\u4f53\u6280\u672f\u96c6\u6210\u7684SDwalker\u5b9e\u73b0\u4e86\u9ad8\u5ea6\u53ef\u7f16\u7a0b\u548c\u53ef\u89c6\u5316\u7684\u6761\u5f62\u7801\u7f16\u7801\u65b9\u6cd5\u3002\uff08iii\uff09\u989c\u8272\u548c\u5f3a\u5ea6\u7f16\u7801\u6761\u5f62\u7801\u662f\u901a\u8fc7\u8c03\u6574\u67d3\u6599\u7684\u989c\u8272\u548c\u8c03\u8282\u54cd\u5e94\u76ee\u6807\u7684SDwalkers\u79cd\u7c7b\u7684\u6570\u91cf\u6765\u6784\u5efa\u7684\uff0c\u8fd9\u514b\u670d\u4e86\u4f20\u7edf\u989c\u8272\u7f16\u7801\u65b9\u6cd5\u7684\u5149\u8c31\u91cd\u53e0\uff0c\u5e76\u7528\u7406\u8bba\u7f16\u7801\u5b9e\u73b0\u7ec6\u83cc\u7684\u8d85\u591a\u91cd\u5206\u6790\uff0c\u7ec6\u83cc\u8868\u578b\u68c0\u6d4b\u548c\u9274\u5b9a\u670920\u79cd\u4e0d\u540c\u7684\u6a21\u5f0f\u3002\u6b64\u5916\uff0c\u901a\u8fc7\u4e0e\u5546\u4e1aFCM\u7684\u6574\u5408\uff0c\u6211\u4eec\u8bc1\u660e\u4e86\u8fd9\u79cd\u65b9\u6cd5\u7684\u53ef\u884c\u6027\uff0c\u4ee5\u53ca\u5728\u5b9e\u9645\u6837\u54c1\u5206\u6790\u4e2d\u7684\u9ad8\u7279\u5f02\u6027\u548c\u8bc6\u522b\u51c6\u786e\u6027\u3002\u56e0\u6b64\uff0c\u8bbe\u60f3\u8be5SDwalker-Drop\u5728\u751f\u7269\u533b\u5b66\u7814\u7a76\u548c\u4e34\u5e8a\u8bca\u65ad\u4e2d\u5177\u6709\u6709\u5e0c\u671b\u7684\u5e94\u7528\u3002<\/p>\n<p><a href=\"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ange.201906438\" target=\"_blank\" rel=\"noopener\">Stochastic DNA Walker in Droplets for Super-Multiplex Bacteria Phenotype Detection<\/a><\/p>\n<p>(Angew. Chem. Int. Ed., 2019, DOI: 10.1002\/anie.201906438)<\/p>\n<p>\u672c\u6587\u7531tt\u4f9b\u7a3f\uff0c\u6750\u6599\u725b\u6574\u7406\u7f16\u8f91\u3002\u00a0<\/p>\n<p>\u6750\u6599\u725b\u7f51\u4e13\u6ce8\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u53ca\u884c\u4e1a\u8fdb\u5c55\uff0c\u8fd9\u91cc\u6c47\u96c6\u4e86\u5404\u5927\u9ad8\u6821\u7855\u535a\u751f\u3001\u4e00\u7ebf\u79d1\u7814\u4eba\u5458\u4ee5\u53ca\u884c\u4e1a\u4ece\u4e1a\u8005\uff0c\u5982\u679c\u60a8\u5bf9\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u8fdb\u5c55\uff0c\u89e3\u8bfb\u9ad8\u6c34\u5e73\u6587\u7ae0\u6216\u662f\u8bc4\u8ff0\u884c\u4e1a\u6709\u5174\u8da3\uff0c\u70b9\u6211\u52a0\u5165\u7f16\u8f91\u90e8\u5927\u5bb6\u5ead\u3002\u00a0<\/p>\n<p>\u6b22\u8fce\u5927\u5bb6\u5230\u6750\u6599\u4eba\u5ba3\u4f20\u79d1\u6280\u6210\u679c\u5e76\u5bf9\u6587\u732e\u8fdb\u884c\u6df1\u5165\u89e3\u8bfb\uff0c\u6295\u7a3f\u90ae\u7bb1tougao@cailiaoren.com\u3002<\/p>\n<p>\u6295\u7a3f\u4ee5\u53ca\u5185\u5bb9\u5408\u4f5c\u53ef\u52a0\u7f16\u8f91\u5fae\u4fe1\uff1acailiaokefu\uff0c\u6211\u4eec\u4f1a\u9080\u8bf7\u5404\u4f4d\u8001\u5e08\u52a0\u5165\u4e13\u5bb6\u7fa4\u3002\u00a0<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u3010\u5f15\u8a00\u3011 \u8d85\u7075\u654f\u68c0\u6d4b\u548c\u75c5\u539f\u4f53\u7684\u5feb\u901f\u9274\u5b9a\u5bf9\u4e8e\u901a\u8fc7\u7528\u9002\u5f53\u7684\u6297\u751f\u7d20\u6cbb\u7597\u6765\u6539\u5584\u60a3\u6709\u7ec6\u83cc\u611f\u67d3\u7684\u60a3\u8005\u7684\u5b58\u6d3b\u7387\u662f\u81f3\u5173\u91cd\u8981\u7684\u3002\u76ee\u524d\u7ec6\u83cc\u8840\u6db2\u57f9\u517b\u7684\u4e34\u5e8a\u6807\u51c6\u9700\u8981\u5927\u91cf\u7684\u8840\u6db2\u6837\u672c\u548c\u7e41\u7410&#8230;<\/p>\n","protected":false},"author":7336,"featured_media":183741,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[215],"tags":[2783],"class_list":["post-183740","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-jiedu","tag-2783"],"_links":{"self":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/183740"}],"collection":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/users\/7336"}],"replies":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=183740"}],"version-history":[{"count":3,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/183740\/revisions"}],"predecessor-version":[{"id":183918,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/183740\/revisions\/183918"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/media\/183741"}],"wp:attachment":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=183740"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=183740"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=183740"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}