{"id":180661,"date":"2019-07-19T06:30:29","date_gmt":"2019-07-18T22:30:29","guid":{"rendered":"\/\/m.iemloyee.com\/?p=180661"},"modified":"2019-07-18T16:22:53","modified_gmt":"2019-07-18T08:22:53","slug":"%e3%80%90%e5%ae%a1%e7%a8%bf%e5%b7%b2%e5%9b%9e%e5%a4%8d%e3%80%91%e6%b5%99%e6%b1%9f%e5%a4%a7%e5%ad%a6%e5%88%98%e5%bb%ba%e9%92%8a%e7%a0%94%e7%a9%b6%e5%91%98%e8%af%be%e9%a2%98%e7%bb%84%e5%92%8c%e5%94%90","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=180661","title":{"rendered":"\u6d59\u5927\u5218\u5efa\u948a\u548c\u5510\u672c\u5fe0\u8bfe\u9898\u7ec4Chem. Commun.: \u901a\u8fc7\u8367\u5149\u548c\u8d28\u8c31\u8054\u7528\u7684\u8868\u5f81\u65b9\u6cd5\u5bf9\u7ec6\u80de\u5185RNA\u7684\u5408\u6210\u4e0e\u964d\u89e3\u8fdb\u884c\u53ef\u89c6\u5316\u548c\u5b9a\u91cf\u5206\u6790"},"content":{"rendered":"

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\u3010\u6210\u679c\u7b80\u4ecb<\/strong>\u3011<\/p>\n

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\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/strong><\/p>\n

Figure 1.<\/strong>\u5728<\/strong>p6A<\/strong>\u4ee3\u8c22\u6807\u8bb0\u7684<\/strong>RNA<\/strong>\u6210\u50cf\u4e2d\uff0c\u5177\u6709<\/strong>AIE<\/strong>\u6d3b\u6027\u7684<\/strong>SO3-TPE-N3<\/strong>\u4e0e\u5546\u4e1a\u67d3\u6599<\/strong>Alexa488-N3<\/strong>\u7684\u5149\u7269\u7406\u6027\u80fd\u7684\u6bd4\u8f83<\/strong><\/p>\n

\"\"<\/p>\n

\uff08A,B\uff09\u6d53\u5ea6\u4e3a10 \u03bcM\u7684Alexa488-N3<\/sub>\u548cSO3<\/sub>-TPE-N3<\/sub>\u7684\u7d2b\u5916\u5438\u6536\u5149\u8c31\u548c\u8367\u5149\u53d1\u5c04\u5149\u8c31<\/p>\n

\uff08C\uff09\u5206\u522b\u7528SO3<\/sub>-TPE-N3<\/sub>\u548cAlexa488-N3<\/sub>\u67d3\u8272\u540e\u7684HeLa\u7ec6\u80de\uff0c\u5728\u4e0d\u540c\u65f6\u95f4\u95f4\u9694\u6fc0\u5149\u626b\u63cf\u540e\uff0c\u7528\u5171\u805a\u7126\u6fc0\u5149\u626b\u63cf\u663e\u5fae\u955c\uff08CLSM\uff09\u6210\u50cf\u7684\u56fe\u50cf\u3002\u6fc0\u53d1\u6ce2\u957f\u5206\u522b\u4e3a405 nm\u548c488 nm; \u6bd4\u4f8b\u5c3a\u4e3a20 \u03bcm\uff1b\u63a2\u9488\u6d53\u5ea6\u4e3a10 \u03bcM<\/p>\n

\uff08D\uff09\u5728\u5171\u805a\u7126\u6fc0\u5149\u626b\u63cf\u663e\u5fae\u955c\u4e0b\u8bb0\u5f55\u7684\u7ec6\u80de\u56fe\u50cf\u7684PL\u5f3a\u5ea6\u4e0e\u626b\u63cf\u65f6\u95f4\u7684\u5173\u7cfb<\/p>\n

Figure 2.<\/strong>\u5728\u4e0d\u540c\u5b9e\u9a8c\u5904\u7406\u6761\u4ef6\u4e0b\uff0c\u8367\u5149\u63a2\u9488\u6807\u8bb0<\/strong>HeLa<\/strong>\u7ec6\u80de\u5185<\/strong>RNA<\/strong>\u7684<\/strong>CLSM<\/strong>\u56fe\u50cf<\/strong><\/p>\n

\"\"<\/p>\n

\uff08A-C\uff09\u5c06\u7ec6\u80de\u4e0ep6<\/sup>A\uff081 mM\uff09\u4e00\u8d77\u57f9\u80b2\uff0c\u7136\u540e\u5229\u7528\u7094-\u53e0\u6c2e\u5316\u7269\u70b9\u51fb\u5316\u5b66\u7528SO3<\/sub>-TPE-N3<\/sub>\u5bf9\u7ec6\u80de\u67d3\u8272<\/p>\n

\uff08D-F\uff09\u5c06\u7ec6\u80de\u7528p6<\/sup>A\uff081 mM\uff09\u548c\u8f6c\u5f55\u6291\u5236\u5242\u653e\u7ebf\u83cc\u7d20D\uff085 \u00b5g\/ mL\uff09\u5b75\u80b26\u5c0f\u65f6\uff0c\u7136\u540e\u8fdb\u884c\u4e0eA-C\u76f8\u540c\u7684\u8367\u5149\u67d3\u8272\u65b9\u6cd5<\/p>\n

\uff08G-I\uff09\u5c06\u7ec6\u80de\u4e0ehydrourea\uff0810 mM\uff09\u4e00\u8d77\u6e29\u80b26\u5c0f\u65f6\uff0c\u5e76\u5e94\u7528\u76f8\u540c\u7684\u67d3\u8272\u65b9\u6cd5\u3002\u84dd\u8272\u901a\u9053\uff1a\u6fc0\u53d1\u6ce2\u957f\uff1a405 nm; \u6536\u96c6\u6ce2\u957f\uff1a420-440 nm; \u9ec4\u8272\u901a\u9053\uff1a\u6fc0\u53d1\u6ce2\u957f\uff1a405 nm; \u91c7\u96c6\u6ce2\u957f\uff1a520-560 nm\u3002\u6bd4\u4f8b\u5c3a\u4e3a20 \u00b5m<\/p>\n

Figure 3.<\/strong>\u4f7f\u7528<\/strong>p6<\/sup>A<\/strong>\u4ee3\u8c22\u6807\u8bb0\u548c<\/strong>SO3<\/sub>-TPE-N3<\/sub><\/strong>\u8367\u5149\u6210\u50cf\u7b56\u7565\u5bf9<\/strong>HeLa<\/strong>\u7ec6\u80de\u4e2d<\/strong>RNA<\/strong>\u5408\u6210\u8fc7\u7a0b\u7684\u7814\u7a76<\/strong><\/p>\n

\"\"<\/p>\n

\uff08A-L\uff09\u5c06HeLa\u7ec6\u80de\u4e0ep6<\/sup>A\uff081 mM\uff09\u4e00\u8d77\u6e29\u80b20, 2, 4\u548c6\u5c0f\u65f6\uff0c\u7136\u540e\u5229\u7528\u53e0\u6c2e\u5316\u7269-\u7094\u70c3\u70b9\u51fb\u5316\u5b66\u7684\u65b9\u6cd5\u7528SO3<\/sub>-TPE-N3<\/sub>\u5bf9p6<\/sup>A\u6807\u8bb0\u7684\u7ec6\u80de\u8fdb\u884c\u67d3\u8272\u3002\u84dd\u8272\u901a\u9053\uff1a\u6fc0\u53d1\u6ce2\u957f\uff1a405 nm; \u91c7\u96c6\u6ce2\u957f\uff1a420-440 nm; \u9ec4\u8272\u901a\u9053\uff1a\u6fc0\u53d1\u6ce2\u957f\uff1a405 nm; \u91c7\u96c6\u6ce2\u957f\uff1a520-560 nm\u3002\u6bd4\u4f8b\u5c3a\u4e3a20 \u00b5m<\/p>\n

\uff08M\uff09\u5168\u7ec6\u80de\u8367\u5149\u5f3a\u5ea6\u4e0e\u4e0d\u540cp6<\/sup>A\u5b75\u80b2\u65f6\u95f4\u4e4b\u95f4\u7684\u51fd\u6570\u5173\u7cfb<\/p>\n

\uff08N\uff09\u4f7f\u7528UPHLC-QQQ-MS\/MS\u6d4b\u5b9a\u7684HeLa\u7ec6\u80de\u4e2d\u5728p6<\/sup>A\u5b58\u5728\u4e0b\u5bf9\u603bRNA p6<\/sup>A\u6444\u5165\u542b\u91cf\u4e0e\u4e0d\u540c\u5b75\u80b2\u65f6\u95f4\u7684\u5b9a\u91cf\u5206\u6790<\/p>\n

\uff08O\uff09p6<\/sup>A\u4e0e\u7ec6\u80de\u57f9\u80b2\u4e0d\u540c\u65f6\u95f4\u540e\u7684UPHLC-QQQ-MS\/MS\u6d4b\u5b9a\uff0c\u68c0\u6d4b\u901a\u9053\u5bf9\u4e8e\u5177\u6709\u8d28\u91cf\u8f6c\u53d8\uff08306\u81f3174\uff09\u7684p6<\/sup>A\u662f\u7279\u5f02\u6027\u7684<\/p>\n

\uff08P\uff09\u5168\u7ec6\u80de\u8367\u5149\u5f3a\u5ea6\u4e0ep6<\/sup>A\/A\u7684\u7ebf\u6027\u76f8\u5173\u6027<\/p>\n

Figure 4.<\/strong>\u4f7f\u7528<\/strong>p6<\/sup>A<\/strong>\u4ee3\u8c22\u6807\u8bb0\u548c<\/strong>SO3<\/sub>-TPE-N3<\/sub><\/strong>\u8367\u5149\u6210\u50cf\u7b56\u7565\u5bf9<\/strong>HeLa<\/strong>\u7ec6\u80de\u4e2d<\/strong>RNA<\/strong>\u964d\u89e3\u8fc7\u7a0b\u7684\u7814\u7a76<\/strong><\/p>\n

\"\"<\/p>\n

\uff08A-L\uff09\u5c06\u7528p6<\/sup>A\uff081 mM\uff09\u9884\u5904\u74066\u5c0f\u65f6\u7684\u7ec6\u80de\u4e0eRNA\u5408\u6210\u6291\u5236\u5242\u4e00\u8d77\u5b75\u80b20, 2, 4\u548c6\u5c0f\u65f6\uff0c\u7136\u540e\u5229\u7528\u53e0\u6c2e\u5316\u7269-\u7094\u70c3\u70b9\u51fb\u5316\u5b66\u7684\u65b9\u6cd5\u7528SO3<\/sub>-TPE-N3<\/sub>\u5bf9p6<\/sup>A\u6807\u8bb0\u7684\u7ec6\u80de\u8fdb\u884c\u67d3\u8272\u3002\u84dd\u8272\u901a\u9053\uff1a\u6fc0\u53d1\u6ce2\u957f\uff1a405 nm; \u91c7\u96c6\u6ce2\u957f\uff1a420-440 nm; \u9ec4\u8272\u901a\u9053\uff1a\u6fc0\u53d1\u6ce2\u957f\uff1a405 nm; \u91c7\u96c6\u6ce2\u957f\uff1a520-560 nm\u3002\u6bd4\u4f8b\u5c3a\u4e3a20 \u00b5m<\/p>\n

\uff08M\uff09\u5168\u7ec6\u80de\u8367\u5149\u5f3a\u5ea6\u4e0e\u4e0d\u540cRNA\u5408\u6210\u6291\u5236\u5242\u5b75\u80b2\u65f6\u95f4\u4e4b\u95f4\u7684\u51fd\u6570\u5173\u7cfb<\/p>\n

\uff08N\uff09\u4f7f\u7528UPHLC-QQQ-MS\/MS\u6d4b\u5b9a\u7684HeLa\u7ec6\u80de\u4e2d\u5728p6<\/sup>A\u5b58\u5728\u4e0b\u5bf9\u603bRNA p6<\/sup>A\u6444\u5165\u542b\u91cf\u4e0e\u4e0d\u540cRNA\u5408\u6210\u6291\u5236\u5242\u5b75\u80b2\u65f6\u95f4\u7684\u5b9a\u91cf\u5206\u6790<\/p>\n

\uff08O\uff09p6<\/sup>A\u4e0eRNA\u5408\u6210\u6291\u5236\u5242\u57f9\u80b2\u4e0d\u540c\u65f6\u95f4\u540e\u7684UPHLC-QQQ-MS\/MS\u6d4b\u5b9a\uff0c\u68c0\u6d4b\u901a\u9053\u5bf9\u4e8e\u5177\u6709\u8d28\u91cf\u8f6c\u53d8\uff08306\u81f3174\uff09\u7684p6<\/sup>A\u662f\u7279\u5f02\u6027\u7684<\/p>\n

\uff08P\uff09\u5168\u7ec6\u80de\u8367\u5149\u5f3a\u5ea6\u4e0ep6<\/sup>A\/A\u7684\u7ebf\u6027\u76f8\u5173\u6027<\/p>\n

\u3010\u5c0f\u7ed3\u3011<\/strong><\/p>\n

\u5728\u8fd9\u4e2a\u5de5\u4f5c\u4e2d\uff0c\u6211\u4eec\u62a5\u5bfc\u4e86\u4e00\u79cd\u8367\u5149\u6210\u50cf\u4e0e\u8d28\u8c31\u68c0\u6d4b\u7ed3\u5408\u7684\u65b9\u6cd5\u7528\u4e8e\u5b9e\u73b0\u7ec6\u80de\u5185RNA\u751f\u7269\u5408\u6210\u4e0e\u964d\u89e3\u7684\u53ef\u89c6\u5316\u548c\u5b9a\u91cf\u5206\u6790\u3002\u8fd9\u79cd\u65b9\u6cd5\u4e3a\u751f\u7269\u5b66\u7814\u7a76RNA\u4ee3\u8c22\u63d0\u4f9b\u4e86\u4e00\u79cd\u7b80\u5355\u3001\u4f4e\u6210\u672c\u7684\u65b9\u6cd5\uff0c\u53ef\u4ee5\u53ca\u65f6\u7814\u7a76\u539f\u4f4dRNA\u7684\u52a8\u6001\u3002\u6807\u8bb0\u7269p6<\/sup>A\u53ef\u4ee5\u901a\u8fc7\u4ee3\u8c22\u8fdb\u5165\u7ec6\u80deRNA\u4e2d\uff0c\u5e76\u53ef\u4ee5\u901a\u8fc7\u8d28\u8c31\u5b9a\u91cf\u68c0\u6d4b\u5176\u542b\u91cf\u3002\u9664\u6b64\u4e4b\u5916\uff0cp6<\/sup>A\u7684\u78b3\u78b3\u4e09\u952e\uff0c\u53ef\u4ee5\u4e0e\u8367\u5149\u63a2\u9488\u4e2d\u7684\u53e0\u6c2e\u57fa\u56e2\u8fdb\u884c\u70b9\u51fb\u53cd\u5e94\uff0c\u5b9e\u73b0\u7ec6\u80de\u5185\u65b0\u5408\u6210\u7684RNA\u7684\u8367\u5149\u6807\u8bb0\u3002\u4e0e\u6613\u4e8e\u5149\u6f02\u767d\u7684\u5546\u4e1aACQ\u63a2\u9488\u76f8\u6bd4\uff0c\u6211\u4eec\u65b0\u5408\u6210\u7684AIE\u8367\u5149\u63a2\u9488SO3<\/sub>-TPE-N3<\/sub>\uff0c\u5728\u751f\u7269\u6c34\u73af\u5883\u4e2d\u5177\u6709\u8f83\u9ad8\u7684\u4eae\u5ea6\u3001\u4f18\u5f02\u7684\u5149\u7a33\u5b9a\u6027\u548c\u8f83\u5927\u7684\u65af\u6258\u514b\u65af\u4f4d\u79fb\u3002\u901a\u8fc7\u9ad8\u7075\u654f\u5ea6\u7684\u8d28\u8c31\u5b9a\u91cf\u9a8c\u8bc1\uff0c\u6211\u4eec\u53d1\u73b0\u5728\u7ec6\u80deRNA\u751f\u7269\u5408\u6210\u548c\u964d\u89e3\u8fc7\u7a0b\u4e2d\u7684\u7ec6\u80de\u8367\u5149\u4fe1\u53f7\u4e0e\u7ec6\u80de\u5185p6<\/sup>A\u6807\u8bb0\u7684RNA\u542b\u91cf\u5448\u8f83\u597d\u7684\u7ebf\u6027\u5173\u7cfb\u3002\u56e0\u6b64\uff0c\u6211\u4eec\u8ba4\u4e3a\u8fd9\u79cd\u8367\u5149\u6210\u50cf\u4e0e\u8d28\u8c31\u7ed3\u5408\u7684\u65b9\u6cd5\u53ef\u4ee5\u5b9e\u73b0\u7ec6\u80de\u5185RNA\u4ee3\u8c22\u7684\u53ef\u89c6\u5316\u548c\u91cf\u5316\u3002<\/p>\n

Visualization and quantification of cellular RNA production and degradation using a combined fluorescence and mass spectrometry characterization assay<\/a><\/p>\n

(Chem. Commun., 2019, DOI: 10.1039\/c9cc03923f)<\/p>\n

\u672c\u6587\u7531\u6750\u6599\u4eba\u5b66\u672f\u7ec4tt\u4f9b\u7a3f\uff0c\u6750\u6599\u725b\u6574\u7406\u7f16\u8f91\u3002\u00a0<\/p>\n

\u6750\u6599\u725b\u7f51\u4e13\u6ce8\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u53ca\u884c\u4e1a\u8fdb\u5c55\uff0c\u8fd9\u91cc\u6c47\u96c6\u4e86\u5404\u5927\u9ad8\u6821\u7855\u535a\u751f\u3001\u4e00\u7ebf\u79d1\u7814\u4eba\u5458\u4ee5\u53ca\u884c\u4e1a\u4ece\u4e1a\u8005\uff0c\u5982\u679c\u60a8\u5bf9\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u8fdb\u5c55\uff0c\u89e3\u8bfb\u9ad8\u6c34\u5e73\u6587\u7ae0\u6216\u662f\u8bc4\u8ff0\u884c\u4e1a\u6709\u5174\u8da3\uff0c\u70b9\u6211\u52a0\u5165\u7f16\u8f91\u90e8\u5927\u5bb6\u5ead\u3002\u00a0<\/p>\n

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\u3010\u5f15\u8a00\u3011 RNA\u5728\u8c03\u8282\u57fa\u56e0\u8868\u8fbe\u4e2d\u8d77\u7740\u81f3\u5173\u91cd\u8981\u7684\u4f5c\u7528\uff0c\u5305\u62ec\u4f20\u9012\u9057\u4f20\u4fe1\u606f\u3001\u611f\u77e5\u6216\u4f20\u9012\u5bf9\u7ec6\u80de\u4fe1\u53f7\u7684\u53cd\u5e94\uff0c\u4ee5\u53ca\u50ac\u5316\u751f\u7269\u53cd\u5e94\u548c\u8868\u89c2\u9057\u4f20\u4fee\u9970\u3002\u7ec6\u80de\u5185RNA\u6c34\u5e73\u7531RNA\u7684\u5408\u6210…<\/p>\n","protected":false},"author":7336,"featured_media":180662,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[215],"tags":[2757],"class_list":["post-180661","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-jiedu","tag-2757"],"_links":{"self":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/180661"}],"collection":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/users\/7336"}],"replies":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=180661"}],"version-history":[{"count":3,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/180661\/revisions"}],"predecessor-version":[{"id":180897,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/180661\/revisions\/180897"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/media\/180662"}],"wp:attachment":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=180661"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=180661"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=180661"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}