{"id":178765,"date":"2019-06-28T06:30:19","date_gmt":"2019-06-27T22:30:19","guid":{"rendered":"\/\/m.iemloyee.com\/?p=178765"},"modified":"2019-06-27T15:28:27","modified_gmt":"2019-06-27T07:28:27","slug":"%e3%80%90%e5%b7%b2%e6%a0%a1%e7%a8%bf%e3%80%91%e6%96%b0%e5%8a%a0%e5%9d%a1%e5%9b%bd%e7%ab%8b%e5%a4%a7%e5%ad%a6%e5%8d%97%e4%ba%ac%e5%b7%a5%e4%b8%9a%e5%a4%a7%e5%ad%a6angew-chem-int-ed-%e9%80%9a","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=178765","title":{"rendered":"\u5357\u4eac\u5de5\u4e1a\u5927\u5b66&\u65b0\u52a0\u5761\u56fd\u7acb\u5927\u5b66Angew. Chem. Int. Ed.:\u901a\u8fc7\u751f\u7269\u53ef\u964d\u89e3\u4e8c\u6c27\u5316\u7845\u7eb3\u7c73\u4f53\u7cfb\u5b9e\u73b0\u5929\u7136\u86cb\u767d\u8d28\u7684\u7ec6\u80de\u5185\u7ebf\u7c92\u4f53\u9776\u5411\u9012\u9001"},"content":{"rendered":"

\u3010\u5f15\u8a00\u3011<\/strong><\/p>\n

\u7ebf\u7c92\u4f53\u662f\u7ec6\u80de\u5185\u91cd\u8981\u7684\u7ec6\u80de\u5668\uff0c\u5b58\u5728\u4e8e\u5927\u591a\u6570\u7684\u771f\u6838\u751f\u7269\uff0c\u6709\u81ea\u8eab\u7684\u9057\u4f20\u7269\u8d28\u7ebf\u7c92\u4f53NDA\uff08mtDNA\uff09\u548c\u86cb\u767d\u8d28\u5408\u6210\u7cfb\u7edf\u3002\u7ebf\u7c92\u4f53\u5728\u7ec6\u80de\u751f\u957f\u548c\u5b58\u6d3b\u4e2d\u53d1\u6325\u91cd\u8981\u4f5c\u7528\uff0c\u80fd\u591f\u4e3a\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u63d0\u4f9b\u52a8\u529b\u548c\u80fd\u91cf\uff0c\u5e76\u53c2\u4e0e\u8bb8\u591a\u4fe1\u53f7\u4f20\u5bfc\u8fc7\u7a0b\u3002\u53e6\u4e00\u65b9\u9762\uff0c\u7ebf\u7c92\u4f53\u529f\u80fd\u969c\u788d\u4e0e\u75be\u75c5\u5bc6\u5207\u76f8\u5173\uff0c\u8bf8\u5982\u764c\u75c7\u3001\u5fc3\u8840\u7ba1\u75be\u75c5\u548c\u795e\u7ecf\u9000\u884c\u6027\u75be\u75c5\u7b49\u3002\u9776\u5411\u7ebf\u7c92\u4f53\u7684\u86cb\u767d\u8d28\u9012\u9001\u88ab\u8ba4\u4e3a\u662f\u6709\u524d\u666f\u7684\u6f5c\u5728\u6cbb\u7597\u7b56\u7565\uff0c\u4f46\u662f\u5411\u53cc\u819c\u7ec6\u80de\u5668\u9012\u9001\u836f\u5242\u662f\u6781\u5177\u6311\u6218\u6027\u7684\u3002\u591a\u5e74\u6765\uff0c\u5df2\u7ecf\u53d1\u5c55\u4e86\u5c06\u5c0f\u5206\u5b50\u548c\u7eb3\u7c73\u6750\u6599\u9012\u9001\u5230\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u7ebf\u7c92\u4f53\u4e2d\u7684\u8bf8\u591a\u65b9\u6cd5\uff0c\u7136\u800c\u8fd9\u4e9b\u65b9\u6cd5\u90fd\u4e0d\u9002\u5408\u4e8e\u9012\u9001\u6cbb\u7597\u6027\u86cb\u767d\u8d28\u548c\u6297\u4f53\u5206\u5b50\u3002\u76ee\u524d\u5927\u591a\u6570\u57fa\u4e8e\u6297\u4f53\u7684\u836f\u7269\u90fd\u9776\u5411\u7ec6\u80de\u8868\u9762\u6216\u5206\u6ccc\u7684\u86cb\u767d\u8d28\uff0c\u663e\u8457\u9650\u5236\u4e86\u5b83\u4eec\u7684\u6f5c\u5728\u5e94\u7528\u3002\u56e0\u6b64\uff0c\u57fa\u4e8e\u86cb\u767d\u8d28\/\u6297\u4f53\u7684\u7597\u6cd5\u4e3b\u8981\u6311\u6218\u5728\u4e8e\u53d1\u5c55\u9ad8\u6548\u7684\u7ec6\u80de\u5185\u86cb\u767d\u8d28\/\u6297\u4f53\u9012\u9001\u7b56\u7565\uff0c\u5b9e\u73b0\u7ec6\u80de\u5feb\u901f\u3001\u9ad8\u6548\u6444\u53d6\u548c\u907f\u514d\u5185\u4f53\u9003\u9038\uff0c\u4ee5\u53ca\u4e9a\u7ec6\u80de\u9776\u5411\u5177\u6709\u91cd\u8981\u7684\u7814\u7a76\u610f\u4e49\u3002\u622a\u6b62\u76ee\u524d\uff0c\u5c06\u5929\u7136\u5916\u6e90\u86cb\u767d\u8d28\u6216\u6297\u4f53\u9012\u9001\u5230\u54fa\u4e73\u52a8\u7269\u6d3b\u7ec6\u80de\u7ebf\u7c92\u4f53\u4e2d\u7684\u7814\u7a76\u8fd8\u672a\u89c1\u62a5\u9053\u3002<\/p>\n

\u3010\u6210\u679c\u7b80\u4ecb\u3011<\/strong><\/p>\n

\u8fd1\u65e5\uff0c\u5357\u4eac\u5de5\u4e1a\u5927\u5b66\u674e\u6797\u6559\u6388<\/strong>\u4e0e\u65b0\u52a0\u5761\u56fd\u7acb\u5927\u5b66\u59da\u5c11\u94a6\u6559\u6388<\/strong>\u5408\u4f5c\u62a5\u9053\u4e86\u5229\u7528\u53ef\u751f\u7269\u964d\u89e3\u7684\u4e8c\u6c27\u5316\u7845\u7eb3\u7c73\u4f53\u7cfb\u5411\u7ebf\u7c92\u4f53\u9012\u9001\u5929\u7136\u86cb\u767d\u8d28\u548c\u6297\u4f53\u3002\u7814\u7a76\u53d1\u73b0\uff0c\u7528\u80fd\u591f\u7a7f\u900f\u7ec6\u80de\u819c\u7684\u805a\u4e8c\u786b\uff08CPD\uff09\u548c\u4e09\u82ef\u57fa\u78f7\uff08TPP\uff09\u4fee\u9970\u7eb3\u7c73\u9897\u7c92\u8868\u9762\u80fd\u591f\u907f\u514d\u6eb6\u9176\u4f53\u7684\u80de\u541e\uff0c\u5927\u5927\u4fc3\u8fdb\u7ec6\u80de\u7684\u6444\u53d6\u548c\u7ebf\u7c92\u4f53\u9776\u5411\u80fd\u529b\uff0c\u4ece\u800c\u4e3a\u6709\u6548\u7684\u7ebf\u7c92\u4f53\u5b9a\u4f4d\u3001\u8c37\u80f1\u7518\u80bd\uff08GSH\uff09\u8bf1\u53d1\u7684\u751f\u7269\u964d\u89e3\u548c\u5929\u7136\u529f\u80fd\u86cb\u767d\u8fdb\u5165\u7ebf\u7c92\u4f53\u63d0\u4f9b\u4e86\u8db3\u591f\u7684\u65f6\u95f4\uff0c\u4e3a\u9776\u5411\u9012\u9001\u751f\u7269\u5927\u5206\u5b50\uff08\u86cb\u767d\u8d28\/\u6297\u4f53\u836f\u7269\uff09\u5230\u7ebf\u7c92\u4f53\u63d0\u4f9b\u4e86\u65b0\u7b56\u7565\u3002\u8be5\u6210\u679c\u4ee5\u9898\u4e3a” Mitochondria-Targeting, Intracellular Delivery of Native Proteins Using Biodegradable Silica Nanoparticles<\/strong>“\u53d1\u8868\u5728\u56fd\u9645\u8457\u540d\u671f\u520aAngew. Chem. Int. Ed.<\/strong>\u4e0a\u3002<\/p>\n

\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/strong><\/p>\n

\u56fe1 CPD-TPP-protein@BS-NPs\u7684\u5236\u5907\u53ca\u5176\u5728\u7ec6\u80de\u7ebf\u7c92\u4f53\u9776\u5411\u9012\u9001\u548cGSH\u523a\u6fc0\u836f\u7269\u91ca\u653e\u7684\u793a\u610f\u56fe<\/strong><\/p>\n

\"\"<\/p>\n

\u56fe2 CPD-TPP-protein@BS-NPs\u751f\u7269\u964d\u89e3\u524d\u5728\u7ec6\u80de\u7ebf\u7c92\u4f53\u4e2d\u7684\u5206\u5e03\u53ca\u5176\u4f53\u5916\u523a\u6fc0\u91ca\u653e<\/strong><\/p>\n

\"\"<\/p>\n

(A) \u5229\u7528MitoTracker\u9884\u5904\u7406\u7136\u540e\u7528\u4e0d\u540c\u7684RB<\/sup>BSA@BS-NPs\u5b75\u531624\u5c0f\u65f6\u7684HeLa\u7ec6\u80de\u7684CLSM\u56fe\u50cf\uff1b<\/p>\n

(B) \u5229\u7528CPD-TPP-RB<\/sup>BSA@BS-NP\u5904\u7406\u7684HeLa\u7ec6\u80de\u7684\u751f\u7269TEM\u56fe\u50cf\uff0810 mg\/mL\uff1b\u5b75\u531624\u621672 h\uff09\uff1b<\/p>\n

(C) \u5229\u7528MitoTracker\u9884\u5904\u7406\u7136\u540e\u4e0eCPD-TPP-FL<\/sup>IgG@BS-NP\uff0810 mg\/mL\uff09\u5b75\u531624\u5c0f\u65f6\u7684HeLa\u7ec6\u80de\u7684CLSM\u56fe\u50cf\uff1b<\/p>\n

(D) \u5728PBS\u4e2d\u5229\u75285 mM GSH\u5b75\u5316\u4e0d\u540c\u65f6\u95f4\uff080\u81f372 h\uff09\u540eFL<\/sup>BSA@BS-NP\u4e0a\u6e05\u6db2\u7684\u8367\u5149\u5149\u8c31\uff1b<\/p>\n

(E) \u4ece\u4e0d\u540cFL<\/sup>BSA@BS-NPs\uff0850 mg\/mL, 72 h\uff09\u5904\u7406\u7684HeLa\u7ec6\u80de\u4e2d\u5206\u79bb\u7684\u7ebf\u7c92\u4f53\u7ea7\u5206\u7684\u8367\u5149\u5149\u8c31\u3002<\/p>\n

\u56fe3 \u7eb3\u7c73\u4f53\u7cfb\u6210\u529f\u9776\u5411\u5230\u7ebf\u7c92\u4f53\u540e\u91ca\u653e\u86cb\u767d\u8d28\/\u6297\u4f53\u7684\u529f\u80fd\u7814\u7a76<\/strong><\/p>\n

\"\"<\/p>\n

(A) MAOA@BS-NP\u7684TEM\u56fe\u50cf\uff1b<\/p>\n

(B) \u7528\u4e0d\u540cMAOA@BS-NPs\uff0850 mg\/mL, 72 h\uff09\u5904\u7406\u7684HepG2\u7ec6\u80de\u7684\u7ebf\u7c92\u4f53\u7ec4\u5206\u7684WB\u5206\u6790\uff1b<\/p>\n

(C) CLSM\u56fe\u50cf\u663e\u793a\u901a\u8fc7\u5df2\u77e5\u5c0f\u5206\u5b50\u63a2\u9488\u68c0\u6d4b\u5230\u7684HepG2\u6216SH-SY5Y\u7ec6\u80de\u4e2d\u7684\u5185\u6e90MAO-A\uff1b<\/p>\n

(D) CLSM\u56fe\u50cf\u663e\u793a\u901a\u8fc7CPD-TPP-MAOA@BS-NP\uff0810 mg\/mL; 72 h\uff09\u5904\u7406\u7ec6\u80de\u7136\u540e\u4e0eMAO-A\u63a2\u9488\u4e00\u8d77\u5b75\u53163\u5c0f\u65f6\u5b9e\u73b0MAO-A\u7684\u6210\u529f\u9012\u9001\u540eHepG2\u7ec6\u80de\u4e2d\u7684\u7ebf\u7c92\u4f53MAO-A\u6d3b\u6027\uff1b<\/p>\n

(E) CLSM\u56fe\u50cf\u663e\u793a\u7528\u6297MTCO2\u6297\u4f53\uff08\u4e0a\u56fe\uff09\u6216\u9634\u6027\u5bf9\u7167\uff08\u4e0b\u56fe\uff1b\u65e0\u6297MTCO2\uff09\u5bf9HeLa\u7ec6\u80de\u8fdb\u884c\u514d\u75ab\u8367\u5149\u67d3\u8272\uff1b<\/p>\n

(F) \u5728\u7528FITC\u6807\u8bb0\u7684\u5c71\u7f8a\u6297\u5154IgG\uff08H + L\uff09\u4e8c\u6297\u56fa\u5b9a\u548c\u5b75\u5316\u524d\uff0c\u7528CPD-TPP-anti-MTCO@BS-NPs\uff0810 mg\/mL\uff0c72 h\uff09\u5904\u7406\u7684\u6d3bHeLa\u7ec6\u80de\u7684CLSM\u56fe\u50cf\u3002<\/p>\n

\u3010\u5c0f\u7ed3\u3011<\/strong><\/p>\n

\u672c\u6587\u4e2d\uff0c\u4f5c\u8005\u4f7f\u7528\u53ef\u751f\u7269\u964d\u89e3\u7684\u4e8c\u6c27\u5316\u7845\u7eb3\u7c73\u4f53\u7cfb\u5b9e\u73b0\u4e86\u5929\u7136\u86cb\u767d\u8d28\/\u6297\u4f53\u7684\u7ec6\u80de\u5185\u7ebf\u7c92\u4f53\u9776\u5411\u9012\u9001\u3002\u8fd9\u4e9b\u8d1f\u8f7d\u86cb\u767d\u8d28\u7684\u7eb3\u7c73\u7c92\u5b50\u80fd\u591f\u88ab\u7ec6\u80de\u5feb\u901f\u3001\u9ad8\u6548\u5730\u6444\u53d6\uff0c\u5728\u9776\u5411\u5bcc\u96c6\u5230\u7ebf\u7c92\u4f53\u540e\uff0c\u8fd9\u4e9b\u4e8c\u6c27\u5316\u7845\u7eb3\u7c73\u7c92\u5b50\u80fd\u591f\u5728GSH\u5b58\u5728\u65f6\u88ab\u964d\u89e3\uff0c\u4ece\u800c\u5b9e\u73b0\u5929\u7136\u529f\u80fd\u6027\u751f\u7269\u5927\u5206\u5b50\u7684\u91ca\u653e\u3002\u5229\u7528\u8fd9\u4e9b\u7ec6\u80de\u5185\u91ca\u653e\u7684\u5927\u5206\u5b50\u53ef\u4ee5\u8fdb\u884c\u66f4\u52a0\u6df1\u5165\u7684\u529f\u80fd\u7814\u7a76\uff0c\u4f7f\u5f97\u8be5\u7b56\u7565\u6709\u671b\u7528\u4e8e\u9488\u5bf9\u4e9a\u7ec6\u80de\u9776\u6807\u7684\u86cb\u767d\u8d28\u6216\u6297\u4f53\u836f\u7269\u7597\u6cd5\u3002<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1aMitochondria-Targeting, Intracellular Delivery of Native Proteins Using Biodegradable Silica Nanoparticles<\/a>\u00a0(Angew. Chem. Int. Ed., 2019, DOI: 10.1002\/anie.201901699)<\/strong><\/p>\n

 <\/p>\n

\u672c\u6587\u7531biotech\u4f9b\u7a3f\uff0c\u6750\u6599\u725b\u5ba1\u6838\u6574\u7406\u3002<\/p>\n

\u6b22\u8fce\u5927\u5bb6\u5230\u6750\u6599\u4eba\u5ba3\u4f20\u79d1\u6280\u6210\u679c\u5e76\u5bf9\u6587\u732e\u8fdb\u884c\u6df1\u5165\u89e3\u8bfb\uff0c\u6295\u7a3f\u90ae\u7bb1tougao@cailiaoren.com\u3002<\/p>\n

\u6295\u7a3f\u53ca\u5185\u5bb9\u5408\u4f5c\u53ef\u52a0\u7f16\u8f91\u5fae\u4fe1\uff1acailiaorenkefu\u3002<\/strong>\u00a0<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

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