{"id":167952,"date":"2019-03-01T06:19:48","date_gmt":"2019-02-28T22:19:48","guid":{"rendered":"\/\/m.iemloyee.com\/?p=167952"},"modified":"2019-02-28T15:20:15","modified_gmt":"2019-02-28T07:20:15","slug":"%e3%80%90%e5%ae%a1%e7%a8%bf%e5%b7%b2%e5%9b%9e%e5%a4%8d%e3%80%91%e5%a8%84%e7%ad%b1%e5%8f%ae%e3%80%81%e5%a4%8f%e5%b8%86%e6%95%99%e6%8e%88%e8%af%be%e9%a2%98%e7%bb%84angew-chem-int-ed-%e5%a4%9a","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=167952","title":{"rendered":"\u5a04\u7b71\u53ee\u3001\u590f\u5e06\u6559\u6388\u8bfe\u9898\u7ec4Angew. Chem. Int. Ed.: \u591a\u529f\u80fd\u6027\u591a\u80bd\u7c7b\u805a\u96c6\u8bf1\u5bfc\u53d1\u5149\u63a2\u9488\u5b9e\u73b0\u57fa\u56e0\u836f\u7269\u9ad8\u6548\u53ef\u63a7\u7684\u7ec6\u80de\u6838\u9776\u5411\u6027\u8fd0\u8f93\u548c\u5b9e\u65f6\u8ffd\u8e2a"},"content":{"rendered":"
\u3010\u5f15\u8a00<\/b><\/strong>\u3011<\/p>\n \u57fa\u56e0\u5e72\u6270\u7597\u6cd5\uff08\u4e5f\u79f0\u4e3a\u57fa\u4e8e\u5be1\u6838\u82f7\u9178\u7684\u7597\u6cd5\uff09\u53ef\u4ee5\u5728\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u4e2d\u7a33\u5b9a\u5730\u6c89\u9ed8\u6216\u6291\u5236\u5e8f\u5217\u7279\u5f02\u6027\u57fa\u56e0\u7684\u8868\u8fbe\u3002\u5176\u5728\u75c5\u539f\u4f53\u3001\u764c\u75c7\u548c\u4ee3\u8c22\u75be\u75c5\u7684\u6cbb\u7597\u4e2d\u83b7\u5f97\u4e86\u663e\u8457\u7684\u6210\u679c\u3002\u5728\u57fa\u4e8e\u5be1\u6838\u82f7\u9178\u7684\u7597\u6cd5\u4e2d\uff0c\u53cd\u4e49\u5355\u94feDNA\u5be1\u6838\u82f7\u9178\uff08ASO\uff09\u7597\u6cd5\u548cRNA\u5e72\u6270\uff08RNAi\uff09\u5728\u8fc7\u53bb\u51e0\u5341\u5e74\u4e2d\u5728\u80de\u8d28\u5185\u548c\u5185\u6838\u5e72\u6270\u65b9\u9762\u53d6\u5f97\u4e86\u663e\u8457\u6210\u679c\u3002\u7136\u800c\uff0c\u7531\u4e8e\u8d1f\u7535\u8377\u548c\u6838\u82f7\u9178\u6613\u4e8e\u9176\u4fc3\u964d\u89e3\u4ee5\u53ca\u751f\u7269\u4f53\u7684\u591a\u79cd\u751f\u7269\u5c4f\u969c\u7684\u9650\u5236\uff0c\u57fa\u4e8e\u5be1\u6838\u82f7\u9178\u7684\u7597\u6548\u53d7\u5230\u4e25\u91cd\u963b\u788d\u3002<\/p>\n \u4e3a\u4e86\u89e3\u51b3\u57fa\u4e8e\u5be1\u6838\u82f7\u9178\u7597\u6cd5\u7684\u5c40\u9650\u6027\uff0c\u591a\u79cd\u8f7d\u4f53\u88ab\u8fdb\u884c\u4e86\u6df1\u5165\u7814\u7a76\u3002\u5176\u4e2d\uff0c\u591a\u80bd\u8fde\u63a5\u7684\u8f7d\u4f53\u7531\u4e8e\u5176\u6709\u5229\u7684\u751f\u7269\u76f8\u5bb9\u6027\u548c\u591a\u529f\u80fd\u7ec4\u4efd\u800c\u88ab\u8ba4\u4e3a\u662f\u4f18\u826f\u7684\u57fa\u56e0\u9012\u9001\u4f53\u7cfb\u4e4b\u4e00\u3002\u8fd9\u4e9b\u590d\u5408\u7269\u901a\u5e38\u901a\u8fc7\u9759\u7535\u76f8\u4e92\u4f5c\u7528\uff0c\u5206\u5b50\u81ea\u7ec4\u88c5\u6216\u5171\u4ef7\u7ed3\u5408\u5f62\u6210\u7a33\u5b9a\u7684\u7eb3\u7c73\u9897\u7c92\u3002\u6b64\u5916\uff0c\u901a\u8fc7\u5229\u7528\u5404\u79cd\u5408\u6210\u80bd\uff0c\u5df2\u7ecf\u5f00\u53d1\u51fa\u7528\u4e8e\u4e9a\u7ec6\u80de\u5668\u7684\u6709\u6548\u57fa\u56e0\u4f20\u9001\u7cfb\u7edf\u3002Tseng\u53ca\u5176\u540c\u4e8b\u4f7f\u7528\u5177\u6709\u591a\u6b65\u4fee\u9970\u7684\u81ea\u7ec4\u88c5\u8d85\u5206\u5b50\u7cfb\u7edf\uff0c\u5305\u542b\u4e0eDNA\u8d28\u7c92\u590d\u5408\u7684\u8f6c\u5f55\u56e0\u5b50\uff0c\u7ec6\u80de\u7a7f\u900f\u80bd\uff0c\u6574\u5408\u578b\u80bd\uff0c\u805a\u4e59\u70ef\u4e9a\u80fa\uff0c\u805a\u4e59\u4e8c\u9187\uff0c\u805a\u9170\u80fa\u80fa\u6811\u679d\u72b6\u5927\u5206\u5b50\uff0c\u80fd\u591f\u5c06DNA\u8d28\u7c92\u4ee5\u7cbe\u786e\u548c\u9ad8\u6548\u7684\u65b9\u5f0f\u4f20\u9012\u5230\u7ec6\u80de\u6838\u3002\u6b64\u5916\uff0c\u5df2\u7ecf\u8bc1\u5b9e\u5177\u6709\u590d\u6742\u548c\u591a\u529f\u80fd\u80bd\u7684\u591a\u7ed3\u6784\u57df\u8bbe\u8ba1\u8f7d\u4f53\u53ef\u4ee5\u901a\u8fc7\u5408\u7406\u6392\u5217\u4fdd\u7559\u5176\u6210\u5206\u7684\u4e2a\u4f53\u529f\u80fd\u3002Hilvert\u53ca\u5176\u540c\u4e8b\u5f00\u53d1\u4e86\u4e00\u79cd\u7b80\u5355\u6709\u6548\u7684\u6a21\u5757\u5316\u7cfb\u7edf\uff0c\u7528\u4e8e\u4f53\u5185\u5305\u88f9RNA\u3002\u7136\u800c\u4e3a\u4e86\u5b9e\u73b0\u9ad8\u6548\u548c\u51c6\u786e\u7684\u6cbb\u7597\uff0c\u5be1\u6838\u82f7\u9178\u7597\u6cd5\u5e94\u8be5\u5c06\u4e9a\u7ec6\u80de\u5668\u9776\u5411\u3001\u6a21\u5757\u5316\u8bbe\u8ba1\u548c\u5b9e\u65f6\u957f\u671f\u8ddf\u8e2a\u4e00\u8d77\u8003\u8651\u3002\u56e0\u6b64\uff0c\u53ef\u5b9e\u73b0\u6709\u6548\u57fa\u56e0\u4f20\u9012\u548c\u5b9e\u65f6\u957f\u671f\u8ffd\u8e2a\u7684\u591a\u529f\u80fd\u591a\u80bd\u8fde\u63a5\u7684\u8f7d\u4f53\u5728\u6cbb\u7597\u4e2d\u5f88\u6709\u9700\u6c42\u3002<\/p>\n \u3010\u6210\u679c\u7b80\u4ecb<\/b><\/strong>\u3011<\/p>\n \u8fd1\u65e5\uff0c\u4e2d\u56fd\u5730\u8d28\u5927\u5b66\uff08\u6b66\u6c49\uff09\u5a04\u7b71\u53ee\u3001\u590f\u5e06\u6559\u6388\u8bfe\u9898\u7ec4<\/b><\/strong>\u62a5\u5bfc\u4e86\u4e00\u79cd\u591a\u529f\u80fd\u7684\u57fa\u56e0\u4f20\u9012\u7b56\u7565\uff0c\u53ef\u4ee5\u4ee5\u9ad8\u6548\u548c\u53ef\u89c6\u5316\u7684\u65b9\u5f0f\u5c06\u6cbb\u7597\u57fa\u56e0\u4f20\u9012\u5230\u9776\u5411\u7684\u7ec6\u80de\u6838\u4e2d\u3002\u4f5c\u8005\u5f00\u53d1\u4e86\u4e00\u79cd\u6574\u5408\u7d20\u9776\u5411\u3001\u7ec6\u80de\u6e17\u900f\u6027\u548c\u6838\u8d28\u4f53\u8fd0\u8f93\u80bd\u8fde\u63a5\u7684AIEgen\uff08TDNCP\uff09\uff0c\u7528\u4e8e\u6709\u6548\u548c\u987a\u5e8f\u9776\u5411\u4f20\u9012\u53cd\u4e49\u5355\u94feDNA\u5be1\u6838\u82f7\u9178\uff08ASO\uff09\uff0c\u5e76\u8fdb\u884c\u5b9e\u65f6\u8ffd\u8e2a\u3002\u4e0eTDNCP\/siRNA-NPs\u76f8\u6bd4\uff0cTDNCP\/ASO-NPs\u8868\u73b0\u51fa\u66f4\u597d\u5730\u5e72\u6270\u6548\u5e94\uff0c\u8fdb\u4e00\u6b65\u8bc1\u660eTDNCP\u662f\u7ec6\u80de\u6838\u9776\u5411\u8f7d\u4f53\u3002\u6b64\u5916\uff0cTDNCP\/ASO-NPs\u5728\u4f53\u5185\u663e\u793a\u51fa\u826f\u597d\u7684\u80bf\u7624\u6291\u5236\u4f5c\u7528\uff0c\u8bc1\u660e\u591a\u529f\u80fd\u80bd\u8fde\u63a5\u7684AIEgen\u53ef\u7528\u4e8e\u987a\u5e8f\u9776\u5411\u57fa\u56e0\u4f20\u9012\uff0c\u5e76\u5b9e\u73b0\u6709\u6548\u7684\u57fa\u56e0\u5e72\u6270\u6cbb\u7597\u3002\u8be5\u6210\u679c\u4ee5\u9898\u4e3a\u201dA Multifunctional Peptide-Conjugated AIEgen for Efficient and Sequential Targeted Gene Delivery into the Nucleus<\/b><\/strong>\u201d\u53d1\u8868\u5728Angew. Chem. Int. Ed.<\/b><\/strong>\u4e0a\u3002<\/p>\n \u534e\u4e2d\u79d1\u6280\u5927\u5b66\u535a\u58eb\u540e\u7a0b\u52c7\u548c\u5b59\u6625\u4e3d\u4ee5\u53ca\u4e2d\u56fd\u5730\u8d28\u5927\u5b66\uff08\u6b66\u6c49\uff09\u535a\u58eb\u540e\u5218\u745e\u4e3a\u8bba\u6587\u7684\u5171\u540c\u7b2c\u4e00\u4f5c\u8005\uff0c\u4e2d\u56fd\u5730\u8d28\u5927\u5b66\uff08\u6b66\u6c49\uff09\u5a04\u7b71\u53ee\u6559\u6388\u548c\u590f\u5e06\u6559\u6388\u4e3a\u8bba\u6587\u7684\u5171\u540c\u901a\u8baf\u4f5c\u8005\u3002\u8be5\u7814\u7a76\u5de5\u4f5c\u5f97\u5230\u4e86\u56fd\u5bb6\u91cd\u70b9\u7814\u53d1\u8ba1\u5212\uff082017YFA0208000, 2016YFF0100800\uff09\u56fd\u5bb6\u81ea\u7136\u79d1\u5b66\u57fa\u91d1\uff0821525523, 21722507, 21574048, 21605053\uff09\u4e2d\u56fd\u535a\u58eb\u540e\u79d1\u5b66\u57fa\u91d1\uff082017M620309, 2017M610492\uff09\u7b49\u9879\u76ee\u7684\u8d44\u52a9\u3002\u00a0<\/b><\/strong><\/p>\n \u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/b><\/strong><\/p>\n Scheme<\/b><\/strong>\u00a0<\/b><\/strong>1<\/b><\/strong>.<\/b><\/strong>TNCP\u548cTNCP\/ASO\u81ea\u7ec4\u88c5\u7684\u7eb3\u7c73\u7c92\u5b50\u7684\u793a\u610f\u56fe<\/b><\/strong><\/p>\n (a).TNCP\u5206\u5b50\u7ed3\u6784<\/p>\n (b).TNCP\/ASO-NPs\u7528\u4e8e\u5b9e\u65f6\u8ffd\u8e2a\u548c\u9776\u5411\u4f20\u9012<\/p>\n Figure <\/b><\/strong>1<\/b><\/strong>.<\/b><\/strong>TNCP\u53ca\u5176\u884d\u751f\u7269\u7684\u8868\u5f81<\/b><\/strong><\/p>\n (a).\u591a\u80bd\u5e8f\u5217<\/p>\n (b).\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\u8868\u5f81<\/p>\n (c,e).DGRKRRRR\u548cRGDKRRRR\u5bf9\u6574\u5408\u7d20\u7684\u5206\u5b50\u7ed3\u5408\u80fd\u529b\u4e0e\u6c22\u952e\u4f5c\u7528<\/p>\n (d,f).\u4e0d\u540c\u63a2\u9488\u4e0e\u6574\u5408\u7d20\u7ed3\u5408\u7684\u8367\u5149\u53d8\u5316<\/p>\n (g,h).\u7528TNCP\u5904\u7406\u7ec6\u80de\u540e\u7684\u7ec6\u80de\u8367\u5149\u5f3a\u5ea6<\/p>\n Figure <\/b><\/strong>2<\/b><\/strong>.<\/b><\/strong>\u7ec6\u80de\u5b9e\u9a8c<\/b><\/strong><\/p>\n (a-c).TNCP\u7684ASO\u8d1f\u8f7d\u80fd\u529b<\/p>\n (d).\u7ec6\u80de\u6210\u50cf<\/p>\n (e).\u7ec6\u80de\u7684\u5e73\u5747\u8367\u5149\u5f3a\u5ea6\u548cPearson\u76f8\u5173\u7cfb\u6570<\/p>\n (f).western blot\u5206\u6790<\/p>\n (g).Bio-TEM\u56fe\u50cf<\/p>\n \u56fe<\/b><\/strong>3<\/b><\/strong>.<\/b><\/strong>\u52a8\u7269\u5b9e\u9a8c<\/b><\/strong><\/p>\n (a).\u5c0f\u9f20\u4f53\u91cd\u53d8\u5316<\/p>\n (b).\u5c0f\u9f20\u80bf\u7624\u4f53\u79ef\u53d8\u5316<\/p>\n (c).Bcl-2\u57fa\u56e0\u5e72\u6270\u6548\u7387<\/p>\n \u3010\u5c0f\u7ed3\u3011<\/b><\/strong><\/p>\n \u5728\u8fd9\u4e2a\u5de5\u4f5c\u4e2d\uff0c\u4f5c\u8005\u5229\u7528\u591a\u529f\u80fd\u80bd\u548cAIEgen\u6765\u5b9e\u73b0\u6709\u6548\u548c\u987a\u5e8f\u9776\u5411\u9012\u9001\u4ee5\u53ca\u8ddf\u8e2a\u7ec6\u80de\u6838\u3002\u901a\u8fc7\u6574\u5408\u7d20\u9776\u5411\u80bd\u3001\u7ec6\u80de\u6e17\u900f\u6027\u80bd\u548c\u6838\u5b9a\u4f4d\u5e8f\u5217\u7684\u7ec4\u5408\uff0cTDNCP\u6bd4\u5176\u4ed6\u884d\u751f\u7269\u663e\u793a\u51fa\u66f4\u4f18\u5f02\u7684\u751f\u7269\u76f8\u5bb9\u6027\u3001\u6838\u9009\u62e9\u6027\u6210\u50cf\u548c\u5149\u7a33\u5b9a\u6027\u3002\u4e0eTDNCP\/siRNA-NPs\uff0clipo\/ASONPs\u548clipo\/siRNA-NPs\u76f8\u6bd4\uff0cTDNCP\/ASO-NPs\u5728\u6574\u5408\u7d20\u8fc7\u8868\u8fbe\u7684\u7ec6\u80de\u7cfb\u4e2d\u663e\u793a\u51fa\u4f18\u5f02\u7684\u57fa\u56e0\u5e72\u6270\u6548\u5e94\u3002\u7531\u4e8e\u987a\u5e8f\u548c\u9776\u5411\u7ec6\u80de\u7a7f\u900f\uff0cTDNCP\/ASO-NPs\u901a\u8fc7\u7624\u5185\u6ce8\u5c04\u663e\u793a\u51fa\u6bd4\u4f53\u5185\u9759\u8109\u6ce8\u5c04\u66f4\u597d\u7684\u5e72\u6270\u6548\u679c\u3002<\/p>\n<\/p>\n
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