{"id":167726,"date":"2019-02-25T06:16:10","date_gmt":"2019-02-24T22:16:10","guid":{"rendered":"\/\/m.iemloyee.com\/?p=167726"},"modified":"2019-02-24T21:55:52","modified_gmt":"2019-02-24T13:55:52","slug":"%e3%80%90%e5%9b%bd%e5%a4%96%e6%9c%aa%e6%a0%a1%e7%a8%bf%e3%80%91%e4%b8%9c%e4%ba%ac%e5%a4%a7%e5%ad%a6%e4%b9%9d%e5%b7%9e%e5%a4%a7%e5%ad%a6%e5%b7%9d%e5%b4%8e%e5%b7%a5%e4%b8%9a%e6%8c%af%e5%85%b4","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=167726","title":{"rendered":"\u4e1c\u4eac\u5927\u5b66&\u4e5d\u5dde\u5927\u5b66&\u5ddd\u5d0e\u5de5\u4e1a\u632f\u5174\u7814\u7a76\u6240J. Am. Chem. Soc.\uff1a\u81ea\u7ec4\u88c5\u805a\u79bb\u5b50\u590d\u5408\u7269\u56ca\u6ce1\u7528\u4e8eRNAi\u548c\u5927\u5206\u5b50\u8d27\u7269\u7684\u5171\u540c\u9012\u9001"},"content":{"rendered":"

\u3010\u5f15\u8a00\u3011<\/strong><\/p>\n

\u805a\u5408\u7269\u56ca\u6ce1\u5177\u6709\u72ec\u7279\u4e2d\u7a7a\u7ed3\u6784\u548c\u53ef\u63a7\u8c03\u8282\u7684\u819c\u7ed3\u6784\uff0c\u56e0\u800c\u805a\u5408\u7269\u56ca\u6ce1\u5df2\u88ab\u8bbe\u8ba1\u7528\u4e8e\u7ec6\u80de\u6a21\u62df\u7269\u3001\u7eb3\u7c73\/\u5fae\u53cd\u5e94\u5668\u4ee5\u53ca\u836f\u7269\u9012\u9001\u8f7d\u4f53\u7b49\u3002\u76ee\u524d\uff0c\u758f\u6c34\u6027\u86cb\u767d\u8d28\u5982\u901a\u9053\u86cb\u767d\u8d28\u548c\u9176\u53ef\u4ee5\u5d4c\u5165\u758f\u6c34\u6027\u805a\u5408\u7269\u56ca\u6ce1\u819c\u4e2d\uff0c\u5206\u522b\u7528\u4e8e\u63a7\u5236\u819c\u6e17\u900f\u6027\u548c\u50ac\u5316\u53cd\u5e94\u3002\u7136\u800c\uff0c\u5728\u56ca\u6ce1\u819c\u4e2d\u6709\u6548\u4e14\u7a33\u5b9a\u5730\u5d4c\u5165\u5f3a\u4eb2\u6c34\u6027\u6216\u5e26\u7535\u8377\u7684\u751f\u7269\u6d3b\u6027\u5927\u5206\u5b50\u4ecd\u7136\u662f\u4e00\u4e2a\u5de8\u5927\u7684\u6311\u6218\u3002\u4e00\u79cd\u89e3\u51b3\u529e\u6cd5\u662f\u5c06\u751f\u7269\u6d3b\u6027\u5927\u5206\u5b50\u4f5c\u4e3a\u81ea\u7ec4\u88c5\u7684\u7ed3\u6784\u57fa\u5143\u800c\u4e0d\u662f\u7b80\u5355\u7684\u5305\u8f7d\u7269\u6765\u5236\u5907\u805a\u5408\u7269\u56ca\u6ce1\uff0c\u5c06\u6b64\u79cd\u65b9\u6cd5\u7528\u4e8e\u5e26\u7535\u751f\u7269\u6d3b\u6027\u5927\u5206\u5b50\u7684\u56ca\u6ce1\u81ea\u7ec4\u88c5\u662f\u975e\u5e38\u5408\u7406\u7684\uff0c\u4e0d\u4ec5\u80fd\u591f\u83b7\u5f97\u5177\u6709\u9ad8\u8d1f\u8f7d\u6548\u7387\u7684\u70ed\u529b\u5b66\u7a33\u5b9a\u7684\u56ca\u6ce1\uff0c\u8fd8\u53ef\u4ee5\u901a\u8fc7\u9002\u5f53\u8c03\u8282\u819c\u7a33\u5b9a\u6027\u5b9e\u73b0\u4f4d\u70b9\u7279\u5f02\u6027\u91ca\u653e\u3002\u5c0f\u5e72\u6270RNA\uff08siRNA\uff09\u662f\u4e00\u79cd\u5e38\u89c1\u7684\u5e26\u8d1f\u7535\u751f\u7269\u6d3b\u6027\u5927\u5206\u5b50\uff0c\u7531\u4e8e\u5176\u5e8f\u5217\u7279\u5f02\u6027\u57fa\u56e0\u6c89\u9ed8\u6d3b\u6027\u5373RNA\u5e72\u6270\uff08RNAi\uff09\uff0c\u8fd1\u51e0\u5341\u5e74\u6765\u53d7\u5230\u4e86\u5e7f\u6cdb\u7684\u7814\u7a76\u5173\u6ce8\u3002\u7136\u800c\u57fa\u4e8e\u5185\u5728\u8106\u5f31\u548c\u5e26\u8d1f\u7535\u6027\u8d28\u7684\u963b\u788d\uff0csiRNA\u7684\u7ec6\u80de\u5185\u5316\u9700\u8981\u8f7d\u4f53\u9012\u9001\u6765\u5b9e\u73b0\u3002<\/p>\n

\u3010\u6210\u679c\u7b80\u4ecb\u3011<\/strong><\/p>\n

\u8fd1\u65e5\uff0c\u4e1c\u4eac\u5927\u5b66<\/strong>Kanjiro Miyata<\/strong>\u6559\u6388\u3001\u4e5d\u5dde\u5927\u5b66<\/strong>Akihiro Kishimura<\/strong>\u6559\u6388\u4e0e\u5ddd\u5d0e\u5de5\u4e1a\u632f\u5174\u7814\u7a76\u6240<\/strong>Kazunori Kataoka<\/strong>\u7814\u7a76\u5458<\/strong>\u5408\u4f5c\uff0c\u9996\u6b21\u4f7f\u7528\u5f3a\u7535\u8377\u3001\u521a\u6027siRNA\u4f5c\u4e3a\u81ea\u7ec4\u88c5\u7ed3\u6784\u57fa\u5143\u6765\u5236\u5907\u56ca\u6ce1\uff0c\u5e76\u901a\u8fc7\u5206\u6790\u56ca\u6ce1\u7684\u5927\u5c0f\u3001\u8868\u9762\u7535\u8377\u3001\u5f62\u8c8c\u548c\u79bb\u805a\u7269\u7ec4\u6210\u7b49\u65b9\u9762\u9a8c\u8bc1\u4e86\u57fa\u4e8esiRNA\u7684\u805a\u79bb\u5b50\u590d\u5408\u7269\u56ca\u6ce1\u7684\u5f62\u6210\u3002\u7136\u540e\u5e7f\u6cdb\u5730\u8003\u5bdf\u4e86siRNA\u57fa\u805a\u5408\u7269\u56ca\u6ce1\u7684\u7ed3\u6784\u7279\u5f81\uff0c\u8fd8\u8bc1\u660e\u4e86siRNA\u57fa\u805a\u5408\u7269\u56ca\u6ce1\u5411\u57f9\u517b\u7ec6\u80de\u9012\u9001siRNA\u7684\u6f5c\u529b\uff0c\u4e3asiRNA\u4e0e\u5176\u4ed6\u4eb2\u6c34\u6027\u751f\u7269\u6d3b\u6027\u5927\u5206\u5b50\u7684\u5171\u540c\u9012\u9001\u63d0\u4f9b\u4e86\u4e00\u4e2a\u591a\u529f\u80fd\u7684\u5e73\u53f0\u3002\u8be5\u6210\u679c\u4ee5\u9898\u4e3a” Self-Assembly of siRNA\/PEG<\/strong>\u2011<\/strong>b<\/strong>\u2011<\/strong>Catiomer at Integer Molar Ratio into 100 nm-Sized Vesicular Polyion Complexes (siRNAsomes) for RNAi and Codelivery of Cargo Macromolecules <\/strong>“\u53d1\u8868\u5728\u56fd\u9645\u8457\u540d\u671f\u520aJ. Am. Chem. Soc.<\/strong>\u4e0a\u3002<\/p>\n

\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/strong><\/p>\n

\u56fe1 \u805a\u79bb\u5b50\u590d\u5408\u7269\u56ca\u6ce1\u7684\u5f62\u6210\u548c\u8154\u4f53\u4e2d\u5927\u5206\u5b50\u8d27\u7269\u7684\u5305\u8986\u793a\u610f\u56fe<\/strong><\/p>\n

\"\"<\/p>\n

\u56fe2 \u805a\u79bb\u5b50\u590d\u5408\u7269\u56ca\u6ce1\u7684\u6d41\u4f53\u52a8\u529b\u5b66\u76f4\u5f84\u548cZeta\u7535\u4f4d\u968fN\/Pfeed<\/sub>\u7684\u53d8\u5316<\/strong><\/p>\n

\"\"<\/p>\n

\u56fe3 \u57fa\u4e8esiRNA\u7684\u805a\u79bb\u5b50\u590d\u5408\u7269\u56ca\u6ce1\u7684\u6a2a\u622a\u9762TEM\u56fe\u50cf<\/strong><\/p>\n

\"\"<\/p>\n

(a) N\/Pfeed<\/sub>\u4e3a1.4\u65f6\u805a\u79bb\u5b50\u590d\u5408\u7269\u56ca\u6ce1\u7684\u6a2a\u622a\u9762TEM\u56fe\u50cf\uff1b<\/p>\n

(b) N\/Pfeed<\/sub>\u4e3a2.2\u65f6\u805a\u79bb\u5b50\u590d\u5408\u7269\u56ca\u6ce1\u7684\u6a2a\u622a\u9762TEM\u56fe\u50cf\u3002<\/p>\n

\u56fe4 \u5728\u4e24\u79cdsiRNAsomes\u72b6\u6001\u4e0b\u805a\u79bb\u5b50\u590d\u5408\u7269\u56ca\u6ce1\u819c\u7684\u793a\u610f\u56fe<\/strong><\/p>\n

\"\"<\/p>\n

\u56fe5 \u5728\u4e0d\u540c[GA]\/[NH2<\/sub>]\u6bd4\u4f8b\u4e0b\u4ea4\u8054siRNAsomes\u7684\u8840\u6e05\u8010\u53d7\u6027<\/strong><\/p>\n

\"\"<\/p>\n

\u56fe6 siRNA\u9012\u9001\u80fd\u529b\u8bc4\u4f30<\/strong><\/p>\n

\"\"<\/p>\n

(a) \u4e0e\u7531siLuc\u6216siScr\u5236\u5907\u5f97\u5230\u7684siRNAsome\u6837\u54c1\u5b75\u531648\u5c0f\u65f6\u540e\uff0cHuh-7-Luc\u7ec6\u80de\u7684\u76f8\u5bf9\u53d1\u5149\u5f3a\u5ea6\uff1b<\/p>\n

(b) \u4e0e\u7531Alexa488-siRNA\u5236\u5907\u5f97\u5230\u7684siRNAsome\u6837\u54c1\u5b75\u531648\u5c0f\u65f6\u540e\uff0cHuh-7-Luc\u7ec6\u80de\u7684\u76f8\u5bf9\u53d1\u5149\u5f3a\u5ea6\uff1b<\/p>\n

(c) \u901a\u8fc7\u5b9e\u65f6qRT-PCR\u6d4b\u5b9a\u5f97\u5230\u7684\uff0cA549\u7ec6\u80de\u4e2d\u4ea4\u8054siRNAsomes\u5728N\/Pfeed<\/sub>\u4e3a 2.2\u548c[GA]\/[NH2]\u4e3a0.4\u6761\u4ef6\u4e0b\u7684\u5185\u6e90\u57fa\u56e0\u6c89\u9ed8\u6548\u7387\u3002<\/p>\n

\u56fe7 siRNAsome\u5305\u8986\u4eb2\u6c34\u6027\u5927\u5206\u5b50\u53ca\u5176\u7ec6\u80de\u9012\u9001<\/strong><\/p>\n

\"\"<\/p>\n

(a) \u7531FCS\u786e\u5b9a\u7684TRITC-Dex\u7684\u81ea\u76f8\u5173\u66f2\u7ebf\uff1b<\/p>\n

(b) \u901a\u8fc7\u6d41\u5f0f\u7ec6\u80de\u672f\u5206\u6790\u786e\u5b9a\u7684A549\u7ec6\u80de\u4e2d\u6e38\u79bbTRITC-Dex\u548cDex@siRNAsomes\u7684\u7ec6\u80de\u6444\u53d6\uff1b<\/p>\n

(c) \u7528Dex @ siRNAsomes\u5b75\u5316\u7684A549\u7ec6\u80de\u7684CLSM\u56fe\u50cf\u3002<\/p>\n

\u3010\u5c0f\u7ed3\u3011<\/strong><\/p>\n

\u672c\u6587\u4e2d\uff0c\u4f5c\u8005\u9996\u6b21\u6210\u529f\u83b7\u5f97\u4e86100\u7eb3\u7c73\u5927\u5c0f\u3001\u5355\u5206\u6563\u4e14\u5176\u819c\u7ed3\u6784\u4e2d\u5305\u8f7d\u4e86siRNA\u7684\u805a\u5408\u7269\u56ca\u6ce1\u3002\u6709\u8da3\u7684\u662f\uff0c\u5728\u8868\u9762\u7535\u8377\u548c\u819c\u539a\u5ea6\u65b9\u9762\uff0c\u53ef\u4ee5\u6e05\u695a\u5730\u89c2\u5bdf\u5230\u4e24\u79cd\u72b6\u6001\u7684siRNA\u57fa\u805a\u5408\u7269\u56ca\u6ce1\uff0c\u5e76\u8fdb\u800c\u8bc1\u5b9e\u5bf9\u4e8e\u7531\u5355\u5206\u6563\u3001\u521a\u6027\u5927\u5206\u5b50\u548c\u67d4\u6027\u5d4c\u6bb5\u5171\u805a\u7269\u5236\u5907\u7684\u805a\u5408\u7269\u56ca\u6ce1\uff0c\u53ef\u4ee5\u8c03\u8282\u5e26\u7535\u5bf9\u7684\u6574\u6570\u6469\u5c14\u6bd4\u6765\u83b7\u5f97\u671f\u671b\u7ed3\u6784\u3002\u800c\u4e14\uff0csiRNA\u57fa\u805a\u5408\u7269\u56ca\u6ce1\u88ab\u8bc1\u660e\u80fd\u591f\u5728\u5176\u8154\u5185\u6355\u83b7\u4eb2\u6c34\u6027\u5927\u5206\u5b50\uff08TRITC-Dex\uff09\u5e76\u7a33\u5b9a\u5730\u5c06\u5b83\u4eec\u4fdd\u6301\u5728\u751f\u7406\u6761\u4ef6\u4e0b\uff0c\u540c\u65f6\u5c06\u5b83\u4eec\u4e0esiRNAs\u9012\u9001\u8fdb\u5165\u57f9\u517b\u7ec6\u80de\u3002\u57fa\u4e8e\u8fd9\u4e9b\u72ec\u7279\u7684\u7279\u6027\uff0csiRNA\u57fa\u805a\u5408\u7269\u56ca\u6ce1\u53ef\u4ee5\u7528\u4e8e\u5728\u819c\u7ed3\u6784\u548c\u5185\u8154\u4e2d\u5305\u8f7d\u5404\u79cd\u7c7b\u578b\u7684\u751f\u7269\u5927\u5206\u5b50\uff0c\u8fd8\u6709\u671b\u4f5c\u4e3a\u591a\u529f\u80fd\u7eb3\u7c73\u8f7d\u4f53\u7528\u4e8e\u8bf8\u5982\u591a\u6a21\u6001\u6cbb\u7597\u548c\u6cbb\u7597\u8bca\u65ad\u7b49\u65b9\u9762\u3002<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1aSelf-Assembly of siRNA\/PEG\u2011b\u2011Catiomer at Integer Molar Ratio into 100 nm-Sized Vesicular Polyion Complexes (siRNAsomes) for RNAi and Codelivery of Cargo Macromolecules<\/a>\u00a0(J. Am. Chem. Soc. 2019, DOI: 10.1021\/jacs.8b13641)<\/strong><\/p>\n

 <\/p>\n

\u672c\u6587\u7531\u6750\u6599\u4eba\u751f\u7269\u5b66\u672f\u7ec4biotech\u4f9b\u7a3f\uff0c\u6750\u6599\u725b\u5ba1\u6838\u6574\u7406\u3002<\/p>\n

\u6b22\u8fce\u5927\u5bb6\u5230\u6750\u6599\u4eba\u5ba3\u4f20\u79d1\u6280\u6210\u679c\u5e76\u5bf9\u6587\u732e\u8fdb\u884c\u6df1\u5165\u89e3\u8bfb\uff0c\u6295\u7a3f\u90ae\u7bb1tougao@cailiaoren.com\u3002<\/p>\n

\u6295\u7a3f\u53ca\u5185\u5bb9\u5408\u4f5c\u53ef\u52a0\u7f16\u8f91\u5fae\u4fe1\uff1acailiaorenkefu\u3002<\/strong>\u00a0<\/p>\n","protected":false},"excerpt":{"rendered":"

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