{"id":155933,"date":"2018-10-11T06:30:37","date_gmt":"2018-10-10T22:30:37","guid":{"rendered":"\/\/m.iemloyee.com\/?p=155933"},"modified":"2018-10-10T14:44:14","modified_gmt":"2018-10-10T06:44:14","slug":"nano-lett-%ef%bc%9a%e6%b0%9f%e5%8c%96%e4%bd%8e%e8%81%9a%e4%b9%99%e7%83%af%e4%ba%9a%e8%83%ba%e7%ba%b3%e7%b1%b3%e7%bb%84%e8%a3%85%e4%bd%93%e5%9b%a0%e5%85%b6%e6%9c%89%e6%95%88%e7%9a%84%e5%86%85%e6%b6%b5","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=155933","title":{"rendered":"Nano Lett.\uff1a\u6c1f\u5316\u4f4e\u805a\u4e59\u70ef\u4e9a\u80fa\u7eb3\u7c73\u7ec4\u88c5\u4f53\u56e0\u5176\u6709\u6548\u7684\u5185\u6db5\u4f53\u9003\u9038\u53ef\u5b9e\u73b0\u8840\u6e05\u73af\u5883\u4e0b\u9ad8\u6548\u7684\u57fa\u56e0\u6c89\u9ed8"},"content":{"rendered":"

\u3010\u5f15\u8a00\u3011<\/strong><\/p>\n

\u5c0f\u5e72\u6270RNA\uff08siRNA\uff09\u4ecb\u5bfc\u7684\u57fa\u56e0\u6cbb\u7597\u5df2\u88ab\u8ba4\u4e3a\u662f\u591a\u79cd\u5148\u5929\u6027\u548c\u6076\u6027\u75be\u75c5\u7684\u4e00\u79cd\u9887\u5177\u524d\u666f\u7684\u6cbb\u7597\u65b9\u6cd5\u3002\u4e3a\u63d0\u9ad8\u8be5\u4f53\u7cfb\u5728\u7279\u5b9a\u7ec4\u7ec7\u7684\u6c89\u9ed8\u6548\u7387\uff0c\u76ee\u524d\u5df2\u5f00\u53d1\u51fa\u5305\u62ec\u91cd\u7ec4\u75c5\u6bd2\u548c\u5408\u6210\u8f7d\u4f53\u4e24\u5927\u7c7b\u57fa\u56e0\u8f7d\u4f53\u3002\u5c3d\u7ba1\u91cd\u7ec4\u75c5\u6bd2\u8868\u73b0\u51fa\u975e\u5e38\u9ad8\u7684siRNA\u9012\u9001\u6548\u7387\uff0c\u4f46\u662f\u7531\u4e8e\u5176\u6f5c\u5728\u7684\u514d\u75ab\u539f\u6027\u3001\u81f4\u764c\u6027\u4ee5\u53ca\u4e0d\u6613\u5927\u89c4\u6a21\u5236\u9020\u7b49\u56f0\u96be\uff0c\u5f88\u5927\u7a0b\u5ea6\u4e0a\u963b\u788d\u4e86\u5176\u5728\u4e34\u5e8a\u4e2d\u7684\u5e94\u7528\u3002\u76f8\u6bd4\u8d77\u6765\uff0c\u5408\u6210\u8f7d\u4f53\uff08\u5982\u9633\u79bb\u5b50\u805a\u5408\u7269\u548c\u8102\u8d28\u4f53\uff09\u5219\u5177\u6709\u514d\u75ab\u539f\u6027\u4f4e\u3001\u6613\u4e8e\u5408\u6210\u548c\u4fee\u9970\u7b49\u4f18\u52bf\u3002\u4f46\u662f\uff0c\u5408\u6210\u8f7d\u4f53\u5728\u542b\u6709\u86cb\u767d\u8d28\u7684\u751f\u7406\u73af\u5883\u91cc\u7684\u57fa\u56e0\u9012\u9001\u80fd\u529b\u5374\u4f1a\u5927\u6253\u6298\u6263\u3002\u56e0\u6b64\uff0c\u8feb\u5207\u9700\u8981\u5f00\u53d1\u80fd\u591f\u5728\u751f\u7406\u73af\u5883\u4e2d\u4ecd\u7136\u5177\u5907\u9ad8\u6548\u9012\u9001\u80fd\u529b\u7684\u5408\u6210\u8f7d\u4f53\u3002\u8fd1\u5e74\u6765\uff0c\u57fa\u4e8e\u5168\u6c1f\u5316\u78b3\u7684\u5408\u6210\u57fa\u56e0\u8f7d\u4f53\u6210\u4e3a\u57fa\u56e0\u9012\u9001\u9886\u57df\u7684\u660e\u661f\u8f7d\u4f53\uff0c\u4e3b\u8981\u662f\u56e0\u4e3a\u5b83\u4eec\u80fd\u591f\u5728\u542b\u6709\u8840\u6e05\u7684\u57f9\u517b\u57fa\u4e2d\u7ef4\u6301\u9ad8\u6548\u7684\u57fa\u56e0\u9012\u9001\u80fd\u529b\u3002\u7136\u800c\uff0c\u5bf9\u4e8e\u5168\u6c1f\u5316\u78b3\u57fa\u56e0\u8f7d\u4f53\u7684\u8fd9\u79cd\u72ec\u7279\u51fa\u4f17\u7684\u57fa\u56e0\u9012\u9001\u80fd\u529b\u7684\u6df1\u5165\u63a2\u7a76\u76ee\u524d\u9c9c\u6709\u62a5\u9053\u3002<\/p>\n

\u3010\u6210\u679c\u7b80\u4ecb\u3011<\/strong><\/p>\n

\u8fd1\u65e5\uff0c\u56fd\u5bb6\u7eb3\u7c73\u79d1\u5b66\u4e2d\u5fc3\u6881\u5174\u6770\u7814\u7a76\u5458\u3001\u5e7f\u5dde\u533b\u79d1\u5927\u5b66<\/strong>\u90ed\u4f1f\u5723\u6559\u6388<\/strong>\u4e0e\u5929\u6d25\u5927\u5b66\u90a2\u91d1\u5cf0\u6559\u6388<\/strong>\u5408\u4f5c\uff0c\u5236\u5907\u4e86\u4e00\u7cfb\u5217\u5168\u6c1f\u8f9b\u9170\u6c2f\u4fee\u9970\u7684\u6c1f\u5316\u4f4e\u805a\u4e59\u70ef\u4e9a\u80fa\u5316\u5408\u7269\uff0c\u6240\u5f97\u5230\u7684\u7eb3\u7c73\u590d\u5408\u7269\u80fd\u591f\u5b9e\u73b0\u7ec6\u80de\u5185\u9ad8\u6548\u7684siRNA\u9012\u9001\u3002\u542b\u8840\u6e05\u57f9\u517b\u57fa\u4e2d\u7684\u57fa\u56e0\u6c89\u9ed8\u5b9e\u9a8c\u8868\u660e\uff0c\u7eb3\u7c73\u590d\u5408\u7269\u5728Luc-HeLa\u7ec6\u80de\u4e2d\u5b9e\u73b0\u4e8688.4%\u7684\u8367\u5149\u7d20\u9176\u6c89\u9ed8\uff0c\u5176\u6c89\u9ed8\u6548\u7387\u7ea6\u4e3a\u5546\u7528\u8f7d\u4f53Lipo 2000\u76842\u500d\u3002\u8fd9\u9879\u5de5\u4f5c\u4e0d\u4ec5\u63d0\u4f9b\u4e86\u4e00\u79cd\u65b0\u578b\u3001\u9ad8\u6548\u7684siRNA\u8f7d\u4f53\uff0c\u800c\u4e14\u8fd8\u5bf9\u6c1f\u5316\u57fa\u56e0\u8f7d\u4f53\u5728\u6709\u8840\u6e05\u6761\u4ef6\u4e0b\u7684\u57fa\u56e0\u9012\u9001\u884c\u4e3a\u548c\u6297\u8840\u6e05\u673a\u5236\u505a\u4e86\u6df1\u5165\u63a2\u7a76\u3002\u4e3a\u7814\u7a76\u6c1f\u5316\u57fa\u56e0\u8f7d\u4f53\u7684\u6297\u8840\u6e05\u673a\u5236\uff0c\u4f5c\u8005\u5f00\u53d1\u4e86\u4e24\u79cd\u70f7\u57fa\u5316\u8f7d\u4f53\uff1aaOEI-C8\u548caOEI-C12\uff0c\u5206\u522b\u4e0e\u6c1f\u5316\u8f7d\u4f53\u5177\u6709\u76f8\u540c\u7684\u5206\u5b50\u7ed3\u6784\u548c\u76f8\u4f3c\u7684\u758f\u6c34\u6bb5\u758f\u6c34\u6027\u3002\u7ec6\u80de\u6c34\u5e73\u7684\u57fa\u56e0\u6c89\u9ed8\u7ed3\u679c\u663e\u793a\uff0c\u4e24\u79cd\u70f7\u57fa\u5316\u8f7d\u4f53\u5728\u8840\u6e05\u5b58\u5728\u4e0b\u7684\u57fa\u56e0\u6c89\u9ed8\u6548\u7387\u5747\u5927\u5e45\u4e0b\u964d\uff0c\u800c\u6c1f\u5316\u8f7d\u4f53\u4ecd\u80fd\u4fdd\u6301\u8f83\u9ad8\u7684\u57fa\u56e0\u6c89\u9ed8\u6548\u7387\u3002\u8be5\u7ed3\u679c\u8bf4\u660e\u6c1f\u5316\u8f7d\u4f53\u7684\u6297\u8840\u6e05\u57fa\u56e0\u9012\u9001\u80fd\u529b\u4e0d\u80fd\u7b80\u5355\u5f52\u56e0\u4e8e\u5176\u5206\u5b50\u7ed3\u6784\u6216\u5206\u5b50\u758f\u6c34\u6027\u3002\u63a5\u4e0b\u6765\uff0c\u4e3a\u5bfb\u6c42\u6c1f\u5316\u8f7d\u4f53\u548c\u70f7\u57fa\u5316\u8f7d\u4f53\u6297\u8840\u6e05\u80fd\u529b\u5dee\u5f02\u7684\u539f\u56e0\uff0c\u4f5c\u8005\u7814\u7a76\u4e86\u5b83\u4eec\u5728\u6709\u3001\u65e0\u8840\u6e05\u4e0b\u7684\u7ec6\u80de\u5185\u541e\u548c\u5185\u6db5\u4f53\u9003\u9038\u884c\u4e3a\u3002\u7ed3\u679c\u663e\u793a\uff0c\u70f7\u57fa\u5316\u8f7d\u4f53\u5728\u8840\u6e05\u5b58\u5728\u6761\u4ef6\u4e0b\uff0c\u5176\u7ec6\u80de\u5185\u541e\u6548\u7387\u867d\u7136\u6709\u6240\u63d0\u9ad8\uff0c\u4f46\u5176\u5185\u6db5\u4f53\u9003\u9038\u80fd\u529b\u663e\u8457\u6027\u964d\u4f4e\uff1b\u800c\u6c1f\u5316\u8f7d\u4f53\u80fd\u591f\u5728\u8840\u6e05\u5b58\u5728\u6761\u4ef6\u4e0b\u4ecd\u7136\u4fdd\u6301\u9ad8\u7684\u5185\u6db5\u4f53\u9003\u9038\u6548\u7387\u3002\u8be5\u7ed3\u679c\u8bf4\u660e\u6c1f\u5316\u8f7d\u4f53\u7684\u6297\u8840\u6e05\u57fa\u56e0\u8f6c\u67d3\u80fd\u529b\u7684\u4e3b\u8981\u539f\u56e0\u4e4b\u4e00\u662f\u5176\u5728\u8840\u6e05\u5b58\u5728\u4e0b\u6240\u4fdd\u6301\u7684\u8f83\u9ad8\u7684\u5185\u6db5\u4f53\u9003\u9038\u80fd\u529b\u3002\u7136\u540e\uff0c\u4f5c\u8005\u8fdb\u4e00\u6b65\u5bf9\u7eb3\u7c73\u7ec4\u88c5\u4f53\u7684\u6297\u86cb\u767d\u5438\u9644\u80fd\u529b\u4ee5\u53ca\u8840\u6e05\u86cb\u767d\u5bf9\u7eb3\u7c73\u7ec4\u88c5\u4f53\u7a33\u5b9a\u6027\u7684\u5f71\u54cd\u8fdb\u884c\u4e86\u7814\u7a76\u3002\u8be5\u7ed3\u679c\u8bf4\u660e\u5373\u4f7f\u6c1f\u5316\u8f7d\u4f53\u5728\u8840\u6e05\u86cb\u767d\u5b58\u5728\u6761\u4ef6\u4e0b\u6240\u4fdd\u6301\u7684\u8f83\u9ad8\u7684\u5185\u6db5\u4f53\u9003\u9038\u80fd\u529b\u662f\u5f97\u76ca\u4e8e\u5176\u4f18\u5f02\u7684\u6297\u86cb\u767d\u5438\u9644\u80fd\u529b\uff0c\u4ece\u800c\u4f7f\u5f97\u5176\u5728\u8840\u6e05\u73af\u5883\u91cc\u4ecd\u7136\u80fd\u591f\u4fdd\u6301\u826f\u597d\u7684\u7a33\u5b9a\u6027\u548c\u8d28\u5b50\u7f13\u51b2\u80fd\u529b\u3002\u6b64\u5916\uff0c\u4f53\u5185\u5b9e\u9a8c\u7ed3\u679c\u663e\u793a\u6c1f\u5316\u8f7d\u4f53\u80fd\u591f\u66f4\u52a0\u5b89\u5168\u6709\u6548\u5730\u6c89\u9ed8\u5c0f\u9f20\u809d\u90e8ApoB\u86cb\u767d\u7684\u8868\u8fbe\uff0c\u4ece\u800c\u964d\u4f4e\u5c0f\u9f20\u8840\u6db2\u4f4e\u5bc6\u5ea6\u8102\u86cb\u767d\u542b\u91cf\u3002\u8be5\u6210\u679c\u4ee5\u9898\u4e3a”Fluorinated Oligoethylenimine Nanoassemblies for Efficient siRNA-Mediated Gene Silencing in Serum-Containing Media by Effective Endosomal Escape<\/strong>“\u53d1\u8868\u5728\u56fd\u9645\u8457\u540d\u671f\u520aNano Letters<\/strong>\u4e0a\u3002\u8be5\u8bba\u6587\u7684\u7b2c\u4e00\u4f5c\u8005\u662f\u5929\u6d25\u5927\u5b66\u5f20\u5ef7\u658c\u535a\u58eb\u548c\u5317\u4eac\u7406\u5de5\u5927\u5b66\u9ec4\u6e0a\u4f59\u6559\u6388\u3002<\/p>\n

\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/strong><\/p>\n

\u56fe1 fx<\/sub>OEI NAs\u7684\u5408\u6210\u4e0e\u8868\u5f81<\/strong><\/p>\n

\"\"<\/p>\n

(a) fx<\/sub>OEIs\u3001fx<\/sub>OEI NAs\u4ee5\u53cafx<\/sub>OEI NAs\u4e0esiRNA\u590d\u5408\u7269\u5236\u5907\u7684\u793a\u610f\u56fe\uff1b<\/p>\n

(b) fx<\/sub>OEI NAs\u4ee5\u53cafx<\/sub>OEI NAs\u4e0esiRNA\u590d\u5408\u7269\u7684TEM\u5f62\u8c8c\u56fe\u7247\uff1b<\/p>\n

(c) \u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u6cd5\u6d4b\u5b9afx<\/sub>OEI NAs\u7684siRNA\u538b\u7f29\u80fd\u529b\u3002<\/p>\n

\u56fe2 fx<\/sub>OEI NAs\u7684\u4f53\u5916siRNA\u8f6c\u67d3<\/strong><\/p>\n

\"\"<\/p>\n

(a) \u4e0d\u540c\u6c1f\u5316\u5ea6\u7eb3\u7c73\u7ec4\u88c5\u4f53\u5728\u7a33\u5b9a\u8868\u8fbe\u8367\u5149\u7d20\u9176\u7684\u4eba\u5bab\u9888\u764cLuc-HeLa\u7ec6\u80de\u4e2d\u7684\u57fa\u56e0\u6c89\u9ed8\u6d4b\u5b9a\uff1b<\/p>\n

(b) \u6bd4\u8f83OEI\u3001Lipo 2000\u4ee5\u53caf0.7<\/sub>OEI NAs\u5728\u6709\u3001\u65e010% (v\/v) FBS\u6761\u4ef6\u4e0b\u6c89\u9ed8HeLa\u7ec6\u80de\u4e2d\u7684GFP\u7684\u80fd\u529b\uff1b<\/p>\n

(c) \u6bd4\u8f83OEI\u3001Lipo 2000\u4ee5\u53caf0.7<\/sub>OEI NAs\u5728\u6709\u3001\u65e010% (v\/v) FBS\u6761\u4ef6\u4e0b\u6c89\u9ed8HeLa\u7ec6\u80de\u4e2d\u7684PLK1\u7684\u80fd\u529b\u3002<\/p>\n

\u56fe3\u70f7\u57fa\u5316\u548c\u6c1f\u5316NAs\u7684\u7269\u7406\u5316\u5b66\u6027\u8d28\u548c\u57fa\u56e0\u6c89\u9ed8\u6548\u7387<\/strong><\/p>\n

\"\"<\/p>\n

(a) aOEI-C8\u3001aOEI-C12\u548cf0.7<\/sub>OEI\u7684\u5316\u5b66\u7ed3\u6784\uff1b<\/p>\n

(b) aOEI-C8 NAs\u3001aOEI-C12 NAs\u548cf0.7<\/sub>OEI NAs\u5728\u6709\u3001\u65e0siRNA\u8d1f\u8f7d\u65f6\u7684TEM\u5f62\u8c8c\u56fe\u7247\uff1b<\/p>\n

(c) aOEI-C8 NAs\u3001aOEI-C12 NAs\u548cf0.7<\/sub>OEI NAs\u7684\u4e34\u754c\u80f6\u675f\u6d53\u5ea6\u548c\u03b6\u7535\u4f4d\u503c\uff1b<\/p>\n

(d) \u901a\u8fc7MTT\u6cd5\u6d4b\u5b9af0.7<\/sub>OEI\u3001aOEI-C8\u548caOEI-C12\u7684NAs\/siRNA\u7eb3\u7c73\u590d\u5408\u7269\u5728\u4e0d\u540cNAs\u6d53\u5ea6\u4e0b\u7684\u7ec6\u80de\u6bd2\u6027\uff1b<\/p>\n

(e) \u6bd4\u8f83aOEI-C8 NAs\u3001aOEI-C12 NAs\u548cf0.7<\/sub>OEI NAs\u5728Luc-HeLa\u7ec6\u80de\u4e2d\u9012\u9001\u9488\u5bf9\u8367\u5149\u7d20\u9176siRNA\u7684\u6548\u7387\u3002<\/p>\n

\u56fe4 \u8840\u6e05\u86cb\u767d\u5bf9\u70f7\u57fa\u5316\u548c\u6c1f\u5316NAs\u9012\u9001\u7684siRNA\u7ec6\u80de\u6444\u53d6\u548c\u5185\u4f53\u9003\u9038\u7684\u5f71\u54cd<\/strong><\/p>\n

\"\"<\/p>\n

(a) \u5229\u7528\u6d41\u5f0f\u7ec6\u80de\u4eea\u6d4b\u5b9aaOEI-C12 NAs\/siRNA\u548cf0.7<\/sub>OEI NAs\/siRNA\u5728\u4e0e\u7ec6\u80de\u5b75\u80b24h\u540e\u7684\u7ec6\u80de\u6444\u53d6\u60c5\u51b5\uff1b<\/p>\n

(b) \u4e0e\u56fea\u7ed3\u679c\u5bf9\u5e94\u7684\u4e0d\u540c\u7ec4\u7684\u5e73\u5747\u8367\u5149\u5f3a\u5ea6\uff1b<\/p>\n

(c)\u5229\u7528\u6fc0\u5149\u5171\u805a\u7126\u626b\u63cf\u663e\u5fae\u955c\uff08CLSM\uff09\u89c2\u5bdfaOEI-C12 NAs\/siRNA\u548cf0.7<\/sub>OEI NAs\/siRNA\u5728\u4e0e\u7ec6\u80de\u5b75\u80b28h\u540e\u7684\u7ec6\u80de\u5185\u5206\u5e03\uff1b<\/p>\n

(d) \u5229\u7528Image-Pro Plus 6.0\u8f6f\u4ef6\u8ba1\u7b97Cy5\u6807\u8bb0siRNA\u548cLysotracker green\u7684\u5171\u5b9a\u4f4d\u6bd4\u4f8b\uff1b<\/p>\n

(e) \u6c2f\u55b9\u548c\u5df4\u5f17\u6d1b\u9709\u7d20A1\u5bf9aOEI-C12 NAs\u548cf0.7<\/sub>OEI NAs\u5728\u6709\u3001\u65e010% (v\/v) FBS\u6761\u4ef6\u4e0b\u6c89\u9ed8Luc-HeLa\u7ec6\u80de\u4e2d\u8367\u5149\u7d20\u9176\u6548\u7387\u7684\u5f71\u54cd\uff1b<\/p>\n

(f) \u5229\u7528\u6fc0\u5149\u5171\u805a\u7126\u626b\u63cf\u663e\u5fae\u955c\uff08CLSM\uff09\u89c2\u5bdfaOEI-C12 NAs\/siRNA\u548cf0.7<\/sub>OEI NAs\/siRNA\u5728\u6709\u3001\u65e0\u6c2f\u55b9\u548c\u5df4\u5f17\u6d1b\u9709\u7d20A1\u60c5\u51b5\u4e0b\u4e0e\u7ec6\u80de\u5b75\u80b28h\u540e\u7684\u7ec6\u80de\u5185\u5206\u5e03\u3002<\/p>\n

\u56fe5 \u8840\u6e05\u86cb\u767d\u5bf9\u70f7\u57fa\u5316\u548c\u6c1f\u5316NAs\u7a33\u5b9a\u6027\u7684\u5f71\u54cd<\/strong><\/p>\n

\"\"<\/p>\n

(a) \u901a\u8fc7BCA\u6cd5\u6d4b\u5b9aaOEI-C12 NAs\u548cf0.7<\/sub>OEI NAs\u5438\u9644\u86cb\u767d\u8d28\u7684\u80fd\u529b\uff1b<\/p>\n

(b) \u901a\u8fc7\u805a\u4e19\u70ef\u9170\u80fa\u51dd\u80f6\u7535\u6cf3\u6d4b\u5b9aaOEI-C12 NAs\u548cf0.7<\/sub>OEI NAs\u5438\u9644\u86cb\u767d\u8d28\u7684\u80fd\u529b\uff1b<\/p>\n

(c) QCM\u6cd5\u6bd4\u8f83aOEI-C12 NAs\u548cf0.7<\/sub>OEI NAs\u5438\u9644BSA\u7684\u80fd\u529b\uff1b<\/p>\n

(d) \u5229\u7528QCM\u5b9e\u65f6\u6d4b\u5b9aBSA\u4e0eaOEI-C12 NAs\u548cf0.7<\/sub>OEI NAs\u7684\u76f8\u4e92\u4f5c\u7528\uff1b<\/p>\n

(e) \u5728\u6709\u3001\u65e010% (v\/ v) FBS\u7684\u57f9\u517b\u57fa\u4e2daOEI-C12 NAs\u548cf0.7<\/sub>OEI NAs\u7684\u80f6\u4f53\u7a33\u5b9a\u6027\uff1b<\/p>\n

(f) \u5728\u6dfb\u52a0FBS\u4e4b\u524d\u548c\u4e0eFBS\u5b75\u5316120\u5206\u949f\u540eaOEI-C12 NAs (1)\u548cf0.7<\/sub>OEI NAs (2)\u6eb6\u6db2\u7684\u56fe\u50cf\uff1b<\/p>\n

(g) \u8840\u6e05\u86cb\u767d\u5728aOEI-C12 NAs\u548cf0.7<\/sub>OEI NAs\u8868\u9762\u4e0a\u5438\u9644\u6548\u7387\u7684\u793a\u610f\u56fe\u3002<\/p>\n

\u56fe6 \u8d1f\u8f7dsiRNA\u7684\u70f7\u57fa\u5316\u548c\u6c1f\u5316NAs\u7684\u4f53\u5185\u5206\u5e03<\/strong><\/p>\n

\"\"<\/p>\n

(a) \u5728C57BL\/6\u5c0f\u9f20\u4e2d\u9759\u8109\u5185\u7ed9\u836fNAs\u540e\u4e0d\u540c\u65f6\u95f4\u70b9\u7684\u5168\u8eab\u56fe\u50cf\uff1b<\/p>\n

(b) \u5728C57BL\/6\u5c0f\u9f20\u4e2d\u9759\u8109\u5185\u7ed9\u836fNAs\u540e\u4e0d\u540c\u65f6\u95f4\u70b9\u7684\u79bb\u4f53\u5668\u5b98\u56fe\u50cf\uff1b<\/p>\n

(c) \u4e0d\u540c\u65f6\u95f4\u70b9\u4e0d\u540c\u7ec4\u6837\u54c1\u5728\u809d\u90e8\u805a\u96c6\u7684\u5e73\u5747\u8367\u5149\u5f3a\u5ea6\uff1b<\/p>\n

(d) \u7ed9\u836f10\u5c0f\u65f6\u540e\u4e3b\u8981\u79bb\u4f53\u7ec4\u7ec7\u7684\u5e73\u5747\u8367\u5149\u5f3a\u5ea6\uff1b<\/p>\n

(e) \u7ed9\u836f30\u5c0f\u65f6\u540e\u4e3b\u8981\u79bb\u4f53\u7ec4\u7ec7\u7684\u5e73\u5747\u8367\u5149\u5f3a\u5ea6\uff1b<\/p>\n

(f) \u7ed9\u836f72\u5c0f\u65f6\u540e\u4e3b\u8981\u79bb\u4f53\u7ec4\u7ec7\u7684\u5e73\u5747\u8367\u5149\u5f3a\u5ea6\u3002<\/p>\n

\u56fe7 \u7528\u8d1f\u8f7d\u6297siApoB\u7684\u70f7\u57fa\u5316\u548c\u6c1f\u5316NAs\u5904\u7406\u7684\u5c0f\u9f20\u7684\u57fa\u56e0\u6c89\u9ed8\u548c\u8840\u6e05\u751f\u5316<\/strong><\/p>\n

\"\"<\/p>\n

(a) \u7528PBS\u3001aOEI-C12\/siApoB NAs\u548cf0.7<\/sub>OEI\/siApoB NAs\u5904\u7406\u540e\u5c0f\u9f20\u809d\u810fApoB mRNA\u6c34\u5e73\u7684\u6d4b\u5b9a\uff1b<\/p>\n

(b) \u7528PBS\u3001aOEI-C12\/siApoB NAs\u548cf0.7<\/sub>OEI\/siApoB NAs\u5904\u7406\u540e\u5c0f\u9f20\u8840\u6e05\u4e2dCHO\u6c34\u5e73\u7684\u6d4b\u5b9a\u7ed3\u679c;<\/p>\n

(c) \u7528PBS\u3001aOEI-C12\/siApoB NAs\u548cf0.7<\/sub>OEI\/siApoB NAs\u5904\u7406\u540e\u5c0f\u9f20\u8840\u6e05\u4e2dLDL-c\u6c34\u5e73\u7684\u6d4b\u5b9a\u7ed3\u679c\uff1b<\/p>\n

(d) \u7528PBS\u3001aOEI-C12\/siApoB NAs\u548cf0.7<\/sub>OEI\/siApoB NAs\u5904\u7406\u540e\u5c0f\u9f20\u8840\u6e05\u4e2dHDL-c\u6c34\u5e73\u7684\u6d4b\u5b9a\u7ed3\u679c;<\/p>\n

(e-h) PBS\u53caaOEI-C12\/siRNA NAs\u548cf0.7<\/sub>OEI\/siRNA NAs\u5728siRNA\u5242\u91cf\u4e3a1\u62163 mg\/kg\u7ec4\u5904\u7406\u5c0f\u9f20\u540e\u7684\u8840\u751f\u5316\u5206\u6790\u7ed3\u679c\u3002<\/p>\n

\u3010\u5c0f\u7ed3\u3011<\/strong><\/p>\n

\u5728\u672c\u6587\u4e2d\uff0c\u4f5c\u8005\u5f00\u53d1\u4e86\u4e00\u7cfb\u5217\u6c1f\u5316\u4f4e\u805a\u4e59\u70ef\u4e9a\u80fa\uff0c\u5176\u80fd\u591f\u5f88\u5bb9\u6613\u5730\u5f62\u6210\u7eb3\u7c73\u7ec4\u88c5\u4f53\uff08fOEI NAs\uff09\uff0c\u5e76\u4e14\u80fd\u591f\u5728\u7ec6\u80de\u4e2d\u6709\u6548\u7684\u9012\u9001siRNA\u3002\u5728\u6ca1\u6709FBS\u7684\u60c5\u51b5\u4e0b\uff0cf0.7<\/sub>OEI NAs\u8868\u73b0\u51fa\u6709\u6548\u7684\u7ec6\u80de\u6444\u53d6\u548c\u5185\u4f53\u9003\u9038\uff0c\u5e76\u4e14\u5373\u4f7f\u5728FBS\u5b58\u5728\u4e0b\u4e5f\u80fd\u591f\u4fdd\u6301\u8fd9\u4e9b\u7279\u6027\u3002\u4e0e\u70f7\u57fa\u5316\u7684NAs\u76f8\u6bd4\uff0cf0.7<\/sub>OEI NAs\u5728\u542b\u6709FBS\u7684\u57f9\u517b\u57fa\u4e2d\u663e\u793a\u51fa\u826f\u597d\u7684\u80f6\u4f53\u7a33\u5b9a\u6027\u548c\u8840\u6e05\u86cb\u767d\u5438\u9644\u6297\u6027\u3002\u6b64\u5916\uff0c\u4e0e\u70f7\u57fa\u5316NAs\u76f8\u6bd4\uff0c\u8f7d\u6709siRNA\u7684f0.7<\/sub>OEI NAs\u5728\u809d\u810f\u4e2d\u5b9e\u73b0\u4e86\u5bf9ApoB\u8868\u8fbe\u7684\u6709\u6548\u6291\u5236\u3002<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1aFluorinated Oligoethylenimine Nanoassemblies for Efficient siRNA-Mediated Gene Silencing in Serum-Containing Media by Effective Endosomal Escape<\/a><\/strong>\u00a0(Nano Lett. 2018, DOI: 10.1021\/acs.nanolett.8b02553)<\/p>\n

 <\/p>\n

\u672c\u6587\u7531\u6750\u6599\u4eba\u751f\u7269\u5b66\u672f\u7ec4biotech\u4f9b\u7a3f\uff0c\u6750\u6599\u725b\u5ba1\u6838\u6574\u7406\u3002<\/p>\n

\u6b22\u8fce\u5927\u5bb6\u5230\u6750\u6599\u4eba\u5ba3\u4f20\u79d1\u6280\u6210\u679c\u5e76\u5bf9\u6587\u732e\u8fdb\u884c\u6df1\u5165\u89e3\u8bfb\uff0c\u6295\u7a3f\u90ae\u7bb1tougao@cailiaoren.com\u3002<\/p>\n

\u6295\u7a3f\u53ca\u5185\u5bb9\u5408\u4f5c\u53ef\u52a0\u7f16\u8f91\u5fae\u4fe1\uff1acailiaorenkefu\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u3010\u5f15\u8a00\u3011 \u5c0f\u5e72\u6270RNA\uff08siRNA\uff09\u4ecb\u5bfc\u7684\u57fa\u56e0\u6cbb\u7597\u5df2\u88ab\u8ba4\u4e3a\u662f\u591a\u79cd\u5148\u5929\u6027\u548c\u6076\u6027\u75be\u75c5\u7684\u4e00\u79cd\u9887\u5177\u524d\u666f\u7684\u6cbb\u7597\u65b9\u6cd5\u3002\u4e3a\u63d0\u9ad8\u8be5\u4f53\u7cfb\u5728\u7279\u5b9a\u7ec4\u7ec7\u7684\u6c89\u9ed8\u6548\u7387\uff0c\u76ee\u524d\u5df2\u5f00\u53d1\u51fa\u5305\u62ec\u91cd\u7ec4…<\/p>\n","protected":false},"author":7746,"featured_media":155934,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[215],"tags":[3611,3610],"class_list":["post-155933","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-jiedu","tag-3611","tag-3610"],"_links":{"self":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/155933"}],"collection":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/users\/7746"}],"replies":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=155933"}],"version-history":[{"count":2,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/155933\/revisions"}],"predecessor-version":[{"id":155949,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/155933\/revisions\/155949"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/media\/155934"}],"wp:attachment":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=155933"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=155933"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=155933"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}