{"id":147427,"date":"2018-07-26T06:30:20","date_gmt":"2018-07-25T22:30:20","guid":{"rendered":"\/\/m.iemloyee.com\/?p=147427"},"modified":"2018-07-31T23:31:55","modified_gmt":"2018-07-31T15:31:55","slug":"%e4%b8%ad%e5%9b%bd%e5%86%9c%e4%b8%9a%e5%a4%a7%e5%ad%a6%e7%bd%97%e4%ba%91%e6%b3%a2%e8%ae%b8%e6%96%87%e6%b6%9b%e5%9b%a2%e9%98%9fchemcommun%e5%86%85%e5%b0%81%e9%9d%a2%e6%96%87%e7%ab%a0%ef%bc%9a%e5%9f%ba","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=147427","title":{"rendered":"\u4e2d\u56fd\u519c\u4e1a\u5927\u5b66\u7f57\u4e91\u6ce2&\u8bb8\u6587\u6d9b\u56e2\u961fChemCommun\u5185\u5c01\u9762\u6587\u7ae0\uff1a\u57fa\u4e8e\u6838\u9178\u7eb3\u7c73\u9636\u68af\u548c\u6c27\u5316\u77f3\u58a8\u70ef\u7684Hg2+\u975e\u9176\u6052\u6e29\u5feb\u901f\u68c0\u6d4b\u6280\u672f"},"content":{"rendered":"

\"\"<\/b><\/strong><\/p>\n

\u3010\u5f15\u8a00\u3011<\/b><\/strong><\/p>\n

Hg2+<\/sup>\u662f\u4e00\u79cd\u5168\u7403\u6027\u91cd\u91d1\u5c5e\u6c61\u67d3\u7269\uff0c\u5b9e\u73b0Hg2+<\/sup>\u7684\u5feb\u901f\u3001\u7075\u654f\u68c0\u6d4b\u5bf9\u73af\u5883\u4fdd\u62a4\u548c\u4eba\u7c7b\u5065\u5eb7\u5177\u6709\u91cd\u8981\u610f\u4e49\u3002\u540c\u5176\u4ed6\u91d1\u5c5e\u79bb\u5b50\u4e00\u6837\uff0cHg2+<\/sup>\u7684\u68c0\u6d4b\u65b9\u6cd5\u4e5f\u7ecf\u5386\u4e86\u7531\u4eea\u5668\u5206\u6790\u5230\u4f20\u611f\u5668\u68c0\u6d4b\u7684\u8fc7\u7a0b\u3002\u4f20\u7edf\u7684\u4eea\u5668\u5206\u6790\u6cd5\u4f8b\u5982\u539f\u5b50\u5438\u6536\/\u53d1\u5c04\u5149\u8c31\u6cd5\uff0c\u539f\u5b50\u8367\u5149\u5149\u8c31\u6cd5\u548c\u8d28\u8c31\u6cd5\u7b49\u5728\u7075\u654f\u5ea6\u548c\u51c6\u786e\u6027\u65b9\u9762\u5177\u6709\u660e\u663e\u7684\u4f18\u52bf\uff0c\u4f46\u6602\u8d35\u7684\u8bbe\u5907\u548c\u7e41\u7410\u7684\u6837\u54c1\u5904\u7406\u8fc7\u7a0b\u4f7f\u5176\u96be\u4ee5\u5e7f\u6cdb\u5e94\u7528\u3002<\/p>\n

\u4f20\u611f\u5668\u68c0\u6d4b\u6cd5\u53ef\u5f25\u8865\u4eea\u5668\u5206\u6790\u6cd5\u7684\u4e0d\u8db3\uff0c\u5177\u6709\u7b80\u5355\u3001\u5feb\u901f\u3001\u4e0d\u9700\u8981\u5927\u578b\u4eea\u5668\u7b49\u4f18\u52bf\u3002\u8fd1\u5e74\u6765\uff0c\u57fa\u4e8e\u529f\u80fd\u6838\u9178\u548c\u7eb3\u7c73\u6750\u6599\u7684\u751f\u7269\u4f20\u611f\u5668\u88ab\u8d8a\u6765\u8d8a\u591a\u7684\u5e94\u7528\u5230Hg2+<\/sup>\u7b49\u91cd\u91d1\u5c5e\u79bb\u5b50\u7684\u5feb\u901f\u68c0\u6d4b\u4e0a\u3002\u8fd9\u7c7b\u4f20\u611f\u5668\u7684\u57fa\u672c\u539f\u7406\u662f\u4fe1\u53f7\u8f6c\u5bfc\uff0c\u91cd\u91d1\u5c5e\u79bb\u5b50\u7684\u5316\u5b66\u4fe1\u53f7\u4f5c\u4e3a\u4fe1\u53f7\u8f93\u5165\uff0c\u5728\u6838\u9178\u3001\u5c0f\u5206\u5b50\u3001\u7535\u5316\u5b66\u6750\u6599\u3001\u91d1\u5c5e\u7eb3\u7c73\u7c92\u5b50\u548c\u86cb\u767d\u9176\u7b49\u7684\u8f85\u52a9\u4e0b\u53ef\u4ee5\u88ab\u8f6c\u5316\u4e3a\u5176\u4ed6\u4fe1\u53f7\u88ab\u8f93\u51fa\uff0c\u4f8b\u5982\u5149\u5b66\u4fe1\u53f7\u548c\u7535\u5316\u5b66\u4fe1\u53f7\u7b49\u3002\u4f46\u662f\u5927\u591a\u6570\u57fa\u4e8e\u5206\u5b50\u751f\u7269\u5b66\u7684\u65b9\u6cd5\u4ecd\u5177\u6709\u4e00\u5b9a\u7684\u5c40\u9650\u6027\uff0c\u56e0\u4e3a\u4f1a\u6d89\u53ca\u5206\u5b50\u4fee\u9970\u6216\u4e00\u4e9b\u9700\u8981\u4f9d\u8d56\u86cb\u767d\u9176\u7684\u8fc7\u7a0b\u3002\u56e0\u6b64\uff0c\u4ecd\u9700\u8981\u66f4\u7b80\u5355\u3001\u66f4\u76f4\u63a5\u7684\u4f20\u611f\u5668\u65b9\u6cd5\u4ee5\u5b9e\u73b0Hg2+<\/sup>\u7684\u5feb\u901f\u68c0\u6d4b\u3002<\/p>\n

\u3010 \u6210\u679c\u7b80\u4ecb\u3011<\/b><\/strong><\/p>\n

\u8fd1\u65e5\uff0c\u4e2d\u56fd\u519c\u4e1a\u5927\u5b66\u7f57\u4e91\u6ce2\u6559\u6388\u548c\u8bb8\u6587\u6d9b\u526f\u6559\u6388\uff08\u5171\u540c\u901a\u8baf\u4f5c\u8005\uff09<\/b><\/strong>\u56e2\u961f\u5728Chemical Communications<\/b><\/strong>\u6742\u5fd7\u5728\u7ebf\u53d1\u8868\u4e86\u4e00\u7bc7\u9898\u4e3a\u201cMercury nanoladders: a new method for DNA amplification, signal identification and their application in the detection of Hg(II) ions<\/b><\/strong>\u201d\u7684\u6587\u7ae0\uff0c\u5efa\u7acb\u4e86\u4e00\u79cd\u57fa\u4e8eHg<\/b><\/strong>2+<\/b><\/sup><\/strong>\u7eb3\u7c73\u9636\u68af<\/b><\/strong>\u548c\u6c27\u5316\u77f3\u58a8\u70ef\uff08GO\uff09<\/b><\/strong>\u7eb3\u7c73\u6750\u6599\u7684Hg2+<\/sup>\u5feb\u901f\u68c0\u6d4b\u7684\u65b9\u6cd5\u3002Hg2+<\/sup>\u7eb3\u7c73\u9636\u68af\u662f\u5efa\u7acb\u5728DNA\u8d85\u5939\u5fc3\u7ed3\u6784\u4e0a\u7684\u4e00\u79cd\u65b0\u7684\u6838\u9178\u7ed3\u6784\u3002\u672c\u7814\u7a76\u4f9d\u9760T-Hg-T\u7ed3\u6784\u5bf9\u8d85\u5939\u5fc3DNA\u8fdb\u884c\u4e86\u6539\u9020\uff0c\u5f62\u6210\u4e00\u79cdHg2+<\/sup>\u62c9\u529b\u8bf1\u5bfc\u7684\u65b0\u578bHg2+<\/sup>\u7eb3\u7c73\u9636\u68af\u7ed3\u6784\u3002\u8fd9\u79cd\u65b0\u578bHg2+<\/sup>\u4f9d\u8d56\u578b\u7ec4\u88c5\u5143\u4ef6\uff0c\u8f85\u4ee5\u8367\u5149\u57fa\u56e2\u4fee\u9970\u8f85\u52a9\uff0c\u9664\u4fe1\u53f7\u653e\u5927\u7684\u4f5c\u7528\u5916\uff0c\u53ef\u4ee5\u4f5c\u4e3aHg2+<\/sup>\u7684\u9776\u6807\u5206\u5b50\u8bc6\u522b\u548c\u4fe1\u53f7\u8f6c\u5316\u5143\u4ef6\u3002<\/p>\n

\u7814\u7a76\u8868\u660e\uff0cGO\u7684\u5927\u03c0\u5171\u8f6d\u7ed3\u6784\u548cDNA\u4e2d\u7684\u78b1\u57fa\u5177\u6709\u5f88\u5f3a\u7684\u03c0-\u03c0\u76f8\u4e92\u4f5c\u7528\uff0c\u56e0\u800c\u5355\u94feDNA\u53ef\u4ee5\u9ad8\u6548\u5438\u9644\u5728GO\u7684\u8868\u9762\uff0c\u4f46\u5bf9\u4e8e\u53cc\u94feDNA\uff0c\u7531\u4e8e\u5177\u6709\u82b3\u9999\u7ed3\u6784\u7684\u78b1\u57fa\u5904\u4e8eDNA\u53cc\u87ba\u65cb\u7ed3\u6784\u7684\u5185\u4fa7\uff0c\u5916\u4fa7\u5e26\u8d1f\u7535\u6027\u7684\u78f7\u9178\u9aa8\u67b6\u5bf9\u5176\u6709\u5c4f\u853d\u4f5c\u7528\uff0c\u4f7f\u5176\u4e0e\u540c\u6837\u5e26\u8d1f\u7535\u6027\u7684GO\u5f7c\u6b64\u53d1\u751f\u9759\u7535\u6392\u65a5\uff0c\u6240\u4ee5\u53cc\u94feDNA\u4e0d\u80fd\u88ab\u7a33\u5b9a\u7684\u5438\u9644\u5728GO\u8868\u9762\u3002\u5229\u7528GO\u5bf9\u5355\u53cc\u94fe\u5438\u9644\u80fd\u529b\u7684\u5dee\u5f02\u6027\u8d28\uff0c\u7ed3\u5408\u8367\u5149\u7d20\u5206\u5b50\u4e0eGO\u4e4b\u95f4\u7684\u8367\u5149\u5171\u632f\u80fd\u91cf\u8f6c\u79fb\uff0c\u5efa\u7acb\u4e86\u201cturn-on\u201d\u578b\u8367\u5149\u4f20\u611f\u5668\u3002\u5728\u65e0Hg2+<\/sup>\u5b58\u5728\u7684\u60c5\u51b5\u4e0b\uff0c\u6ca1\u6709Hg2+<\/sup>\u7684\u8bf1\u5bfc\uff0c\u4e0d\u80fd\u5f62\u6210T-Hg-T\u7ed3\u6784\uff0c\u7eb3\u7c73\u9636\u68af\u4e0d\u80fd\u5f62\u6210\uff0cGO\u5c06\u6dec\u706d\u6807\u8bb0\u6709\u8367\u5149\u5206\u5b50\u7684\u5355\u94fe\u5f15\u7269\u7684\u8367\u5149\u4fe1\u53f7\u3002\u5728Hg2+<\/sup>\u5b58\u5728\u7684\u60c5\u51b5\u4e0b\uff0cHg2+<\/sup>\u53ef\u4ee5\u6210\u529f\u8bf1\u5bfc\u7eb3\u7c73\u9636\u68af\u7684\u5f62\u6210\uff0cGO\u4e0d\u80fd\u6dec\u706d\u751f\u6210\u7684\u7eb3\u7c73\u9636\u68af\u4e0a\u7684\u8367\u5149\u5206\u5b50\u7684\u8367\u5149\u4fe1\u53f7\uff0c\u5b9e\u73b0Hg2+<\/sup>\u7684\u5feb\u901f\u9ad8\u7075\u654f\u68c0\u6d4b\u3002\u4e0e\u4ee5\u524d\u62a5\u9053\u7684\u6280\u672f\u76f8\u6bd4\uff0cHg2+<\/sup>\u7eb3\u7c73\u9636\u68af-GO\u4f20\u611f\u5668\u80fd\u591f\u572840 min<\/b><\/strong>\u4e4b\u5185\u5b9e\u73b0Hg2+<\/sup>\u7684\u5b9a\u91cf\u68c0\u6d4b\u3002<\/p>\n

\u00a0<\/b><\/strong>\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/b><\/strong><\/p>\n

\u00a0<\/b><\/strong>\u56fe1. Hg<\/b><\/strong>2+<\/b><\/sup><\/strong>\u7eb3\u7c73\u53caGO\u7684\u8868\u5f81<\/b><\/strong>\u00a0<\/b><\/strong><\/p>\n

\"\"<\/p>\n

\uff08a\uff09\u7528\u5706\u4e8c\u8272\u8c31\uff08CD\uff09\u8868\u5f81\u6709\u65e0Hg2+<\/sup>\u65f6Hg2+<\/sup>\u00a0\u7eb3\u7c73\u9636\u68af\u4e8c\u7ea7\u7ed3\u6784\u7684\u53d8\u5316\uff0c\u5176\u4e2d\u84dd\u8272\u7684\u66f2\u7ebf\u4ee3\u8868\u6ca1\u6709Hg2+<\/sup>\u5b58\u5728\uff1b\u7ea2\u8272\u7684\u66f2\u7ebf\u4ee3\u8868\u6709Hg2+<\/sup>\u5b58\u5728\uff1b\u7eff\u8272\u7684\u66f2\u7ebf\u4ee3\u8868Tris-HCl Buffer\u3002<\/p>\n

\uff08b\uff09GO\u5728\u539f\u5b50\u529b\u663e\u5fae\u955c\uff08AFM\uff09\u4e0b\u7684\u8868\u5f81\uff0c\u6807\u5c3a500nm\u3002<\/p>\n

\u00a0<\/b><\/strong>\u56fe2. Hg<\/b><\/strong>2+<\/b><\/sup><\/strong>\u7eb3\u7c73\u9636\u68af\u7f13\u51b2\u6db2\u4f18\u5316<\/b><\/strong><\/p>\n

\"\"<\/p>\n

\uff08a\uff09\u7f13\u51b2\u6db2\u79cd\u7c7b\u4f18\u5316\uff1b<\/p>\n

\uff08b\uff09Tris-HCl Buffer \u4e2dTris\u542b\u91cf\u4f18\u5316\uff1b<\/p>\n

\uff08c\uff09Tris-HCl Buffer \u4e2dNaCl\u542b\u91cf\u4f18\u5316\uff1b<\/p>\n

\uff08d\uff09Tris-HCl Buffer \u4e2dMg2+<\/sup>\u542b\u91cf\u4f18\u5316\u3002<\/p>\n

\u56fe3. Hg<\/b><\/strong>2+<\/b><\/sup><\/strong>\u7eb3\u7c73\u9636\u68af\u5b75\u80b2\u65f6\u95f4\u53ca\u5f15\u7269P1\u6d53\u5ea6\u4f18\u5316<\/b><\/strong><\/p>\n

\"\"<\/p>\n

\uff08a-d\uff09GO\u5bf9\u4e0d\u540c\u5b75\u80b2\u65f6\u95f4\u3001\u4e0d\u540c\u6d53\u5ea6\u5f15\u7269P1\u642d\u8f7d\u7684\u7eb3\u7c73\u9636\u68af10 nM (a) 20 nM (b) 50 nM (c)\u548c100 nM (d)\u7684\u8367\u5149\u6dec\u706d\u6548\u7387\u66f2\u7ebf\u56fe\uff0c\u5176\u4e2d\u84dd\u8272\u7684\u66f2\u7ebf\u4ee3\u8868P1+ P2\uff1b\u7ea2\u8272\u7684\u66f2\u7ebf\u4ee3\u8868P1+ P2 +Hg2+<\/sup>,\uff1b\u7eff\u8272\u7684\u66f2\u7ebf\u4ee3\u8868P1 +T1\u3002<\/p>\n

\u56fe4. Hg<\/b><\/strong>2+<\/b><\/sup><\/strong>\u7684\u5b9a\u91cf\u4e0e\u7279\u5f02\u6027\u5206\u6790<\/b><\/strong><\/p>\n

\"\"<\/p>\n

\uff08a\uff09Hg2+<\/sup>\u7eb3\u7c73\u9636\u68af-GO\u4f20\u611f\u5668\u68c0\u6d4b\u533a\u95f4\u53ca\u6807\u51c6\u66f2\u7ebf\uff1b<\/p>\n

\uff08b\uff09Hg2+<\/sup>\u7eb3\u7c73\u9636\u68af-GO\u4f20\u611f\u5668\u7279\u5f02\u6027\u5206\u6790\u3002<\/p>\n

\u3010\u5c0f\u7ed3\u3011<\/b><\/strong><\/p>\n

\uff081\uff09 \u672c\u7814\u7a76\u901a\u8fc7\u5f15\u5165\u529f\u80fd\u6838\u9178\u6750\u6599Hg2+<\/sup>\u7eb3\u7c73\u9636\u68af\uff0c\u7ed3\u5408\u7eb3\u7c73\u6750\u6599\u6c27\u5316\u77f3\u58a8\u70ef\uff08GO\uff09\uff0c\u5efa\u7acb\u4e86\u80fd\u591f\u5728\u65e0\u9176\u3001\u6052\u6e29\u6761\u4ef6\u4e0b\u5b9e\u73b0Hg2+<\/sup>\u5b9a\u91cf\u68c0\u6d4b\u7684\u751f\u7269\u4f20\u611f\u5668\u3002<\/p>\n

\uff082\uff09 \u901a\u8fc7\u5bf9Hg2+<\/sup>\u7eb3\u7c73\u9636\u68af\u5e8f\u5217\u3001\u7f13\u51b2\u73af\u5883\u3001\u5f15\u7269\u6d53\u5ea6\u7b49\u56e0\u7d20\u7684\u8c03\u6574\u548c\u4f18\u5316\uff0c\u8bba\u8bc1\u4e86Hg2+<\/sup>\u7eb3\u7c73\u9636\u68af\u7ed3\u6784\u76f8\u8f83\u4e8e\u4f20\u7edf\u53cc\u94fe\u7ed3\u6784\u5728GO\u4f53\u7cfb\u4e2d\u5177\u6709\u66f4\u9ad8\u7684\u7075\u654f\u5ea6\uff0c\u8bba\u8bc1\u4e86\u642d\u8f7dHg2+<\/sup>\u7eb3\u7c73\u9636\u68af\u7684\u5fc5\u8981\u6027\u3002<\/p>\n

\uff083\uff09 \u8be5\u751f\u7269\u4f20\u611f\u5668\u5b9e\u73b0\u4e86Hg2+<\/sup>\u7684\u5feb\u901f\u68c0\u6d4b\uff0c\u68c0\u6d4b\u65f6\u957f40 min\u3002\u5b9a\u91cf\u68c0\u6d4b\u9650\u4e3a1.5 nM\uff0c\u68c0\u6d4b\u533a\u95f4\u4e3a0~20 nM\uff0c\u5177\u6709\u826f\u597d\u7684\u7279\u5f02\u6027\uff0c\u4e14\u80fd\u591f\u7528\u4e8e\u5b9e\u9645\u6837\u54c1\u7684\u68c0\u6d4b\u3002<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1a<\/strong>Mercury nanoladders: a new method for DNA amplification, signal identification and their application in the detection of Hg (II) ions (Chem. Commun.<\/i><\/em>, 2018, 54, 8036, DOI: 10.1039\/c8cc03851a)<\/p>\n

\u3010\u901a\u8baf\u4f5c\u8005\u53ca\u56e2\u961f\u7b80\u4ecb\u3011<\/b><\/strong><\/p>\n

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