{"id":144560,"date":"2018-07-04T14:00:49","date_gmt":"2018-07-04T06:00:49","guid":{"rendered":"\/\/m.iemloyee.com\/?p=144560"},"modified":"2018-07-02T14:57:11","modified_gmt":"2018-07-02T06:57:11","slug":"sci-adv-%e9%9d%9e%e4%ba%ba%e7%b1%bb%e7%81%b5%e9%95%bf%e7%b1%bb%e5%8a%a8%e7%89%a9%e4%bd%bf%e7%94%a8%e5%8f%af%e7%94%b5%e7%a6%bb%e7%9a%84%e4%bd%8e%e5%88%86%e5%ad%90%e9%87%8f%e8%81%9a%e5%90%88","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=144560","title":{"rendered":"Sci. Adv.:  \u975e\u4eba\u7c7b\u7075\u957f\u7c7b\u52a8\u7269\u4f7f\u7528\u53ef\u7535\u79bb\u7684\u4f4e\u5206\u5b50\u91cf\u805a\u5408\u7269\u7eb3\u7c73\u9897\u7c92\u7684\u5185\u76aesiRNA\u4f20\u9012"},"content":{"rendered":"<p><strong>\u3010\u5f15\u8a00\u3011<\/strong><\/p>\n<p>\u5065\u5eb7\u7684\u5185\u76ae\u7ec6\u80de\u5bf9\u4e8e\u91cd\u8981\u7684\u751f\u7406\u529f\u80fd\u662f\u5fc5\u4e0d\u53ef\u5c11\u7684\uff0c\u5305\u62ec\u6b62\u8840\u3001\u514d\u75ab\u53cd\u5e94\u3001\u7ec4\u7ec7\u751f\u957f\u548c\u8840\u7ba1\u8c03\u8282\u3002\u7136\u800c\uff0c\u529f\u80fd\u4e0d\u5168\u7684\u5185\u76ae\u662f\u8bb8\u591a\u75be\u75c5\u7684\u6807\u5fd7\uff0c\u5305\u62ec\u5468\u56f4\u8840\u7ba1\u75be\u75c5\u3001\u4e2d\u98ce\u3001\u9ad8\u8840\u538b\u3001\u52a8\u8109\u7ca5\u6837\u786c\u5316\u3001\u7cd6\u5c3f\u75c5\u3001\u5668\u5b98\u8870\u7aed\u3001\u67d0\u4e9b\u7c7b\u578b\u7684\u611f\u67d3\u548c\u764c\u75c7\u3002\u5bf9\u4e8e\u8fd9\u4e9b\u75be\u75c5\u4e2d\u7684\u8bb8\u591a\u6709\u6548\u7684\u4ee5\u5185\u76ae\u7ec6\u80de\u4e3a\u4e2d\u5fc3\u7684\u6cbb\u7597\u65b9\u6cd5\u53ef\u4ee5\u6539\u5584\u60a3\u8005\u7684\u75c5\u51b5\u3002\u5176\u4e2d\u4e00\u79cd\u65b9\u6cd5\u5c31\u662fRNA\u5e72\u6270(RNAi)\uff0c\u4e00\u79cd\u4f7f\u7528\u5c0f\u5e72\u6270RNA (siRNA)\u7684\u6280\u672f\u3002RNAi\u53ef\u7528\u4e8e\u6291\u5236\u5185\u76ae\u4e2d\u5f02\u5e38\u57fa\u56e0\u7684\u8868\u8fbe\u3002\u7136\u800c\uff0c\u8981\u5145\u5206\u53d1\u6325\u8fd9\u7c7b\u5e72\u9884\u7684\u6cbb\u7597\u6f5c\u529b\uff0c\u9996\u5148\u5fc5\u987b\u786e\u4fddsiRNA\u7684\u4f18\u5148\u8f93\u9001\u5230\u5185\u76ae\u7ec6\u80de\u3002\u5c3d\u7ba1\u6700\u8fd1\u7684\u4e00\u4e9b\u62a5\u544a\u663e\u793a\u4e86\u7eb3\u7c73\u9897\u7c92\u7684\u53d1\u5c55\u72b6\u51b5\uff0c\u4e14\u7eb3\u7c73\u9897\u7c92\u80fd\u591f\u5728\u975e\u4eba\u7c7b\u7075\u957f\u7c7b\u52a8\u7269\u548c\u4eba\u4f53\u5185\u7cfb\u7edf\u6ce8\u5c04siRNA\uff0c\u4f46\u662f\u8fd9\u4e9b\u7eb3\u7c73\u9897\u7c92\u4e3b\u8981\u5c06siRNA\u4f20\u9012\u7ed9\u809d\u7ec6\u80de\u548c\u9aa8\u9ad3\u7ec6\u80de\uff0c\u800c\u4e0d\u662f\u5185\u76ae\u7ec6\u80de\u3002\u6545\u800c\uff0c\u4e00\u79cd\u7eb3\u7c73\u7c92\u5b50\u7cfb\u7edf\u80fd\u591f\u5728\u5168\u8eab\u7528\u836f\u4e4b\u540e\uff0c\u80fd\u5728\u591a\u4e2a\u975e\u4eba\u7c7b\u7075\u957f\u7c7b\u5668\u5b98(\u80ba\u810f\u3001\u5fc3\u810f\u3001\u80be\u810f\u548c\u89c6\u7f51\u819c)\u7684\u5185\u76ae\u7ec6\u80de\u4e2d\u83b7\u5f97\u9009\u62e9\u6027\u7684siRNA\u662f\u4e00\u4e2a\u597d\u7684\u7814\u7a76\u65b9\u5411\u3002<\/p>\n<p><strong>\u3010\u6210\u679c\u7b80\u4ecb\u3011<\/strong><\/p>\n<p>\u8fd1\u65e5\uff0c<strong>\u7f8e\u56fd\u7684\u6234\u7ef4\u00b7h\u00b7\u79d1\u8d6b\u7efc\u5408\u764c\u75c7\u7814\u7a76\u6240\u7684Piotr S. Kowalski\u6559\u6388\u548cJoshua C. Doloff\u6559\u6388\uff08\u5171\u540c\u901a\u8baf\u4f5c\u8005\uff09<\/strong>\u7b49\u62a5\u9053\u4e86\u901a\u8fc7\u5c06\u4f4e\u5206\u5b50\u3001\u53ef\u5f62\u6210\u7eb3\u7c73\u9897\u7c92\u7684\u53ef\u7535\u79bb\u805a\u5408\u72697C1\u4f20\u9012\u7ed9\u5c0f\u5e72\u6270RNA (siRNA)\uff0c\u5b9e\u73b0\u4e86\u975e\u4eba\u7c7b\u7075\u957f\u7c7b\u52a8\u7269\u5185\u76ae\u7ec6\u80de\u6709\u6548\u7684\u57fa\u56e0\u6c89\u9ed8\u3002\u5728\u6bcf\u516c\u65a4\u52a8\u7269\u9759\u8109\u6ce8\u5c041 mg siRNA\u540e\uff0c7C1\u7eb3\u7c73\u9897\u7c92\u9012\u9001Tie2 siRNA\u5bfc\u81f4\u80ba\u5185\u76ae\u4e2dTie2 mRNA\u7684\u6c34\u5e73\u4e0b\u964d\u4e86\u5927\u7ea680%\u3002\u5728\u5fc3\u810f\u3001\u89c6\u7f51\u819c\u3001\u80be\u810f\u3001\u80f0\u817a\u548c\u9aa8\u9abc\u4e2d\u4e5f\u53d1\u73b0\u4e86Tie2 mRNA\u7684\u663e\u8457\u4e0b\u964d\u3002\u6cbb\u7597\u524d\u540e\u8840\u6db2\u5316\u5b66\u53ca\u809d\u529f\u80fd\u5206\u6790\u5747\u663e\u793a\u86cb\u767d\u53ca\u9176\u6d53\u5ea6\u5747\u5728\u6b63\u5e38\u53c2\u8003\u503c\u8303\u56f4\u5185\u3002\u6b64\u5916\uff0c\u5728\u63a7\u5236\u4e86sirna\u7279\u5f02\u6027\u4f5c\u7528\u540e\uff0c\u8840\u6e05\u4e2d\u708e\u75c7\u7ec6\u80de\u56e0\u5b50\u6d53\u5ea6\u6ca1\u6709\u663e\u8457\u589e\u52a0\u3002\u540c\u6837\uff0c\u5728\u5bf9\u975e\u4eba\u7c7b\u7075\u957f\u7c7b\u7ec4\u7ec7\u8fdb\u884c\u7ec4\u7ec7\u5b66\u68c0\u67e5\u540e\uff0c\u6ca1\u6709\u53d1\u73b0\u4efb\u4f55\u4e25\u91cd\u7684\u75c5\u53d8\u6216\u660e\u663e\u7684\u6f5c\u5728\u75c5\u7406\u3002\u8fd9\u9879\u7814\u7a76\u662f\u9996\u6b21\u4f7f\u7528\u5168\u8eab\u7528\u836f\u7684siRNA\u7eb3\u7c73\u9897\u7c92\u5728\u591a\u4e2a\u975e\u4eba\u7075\u957f\u7c7b\u52a8\u7269\u5668\u5b98\u4e2d\u8bc1\u5b9e\u5185\u76ae\u57fa\u56e0\u6c89\u9ed8\uff0c\u5e76\u5f3a\u8c03\u4e86\u8fd9\u79cd\u65b9\u6cd5\u5728\u6cbb\u7597\u4eba\u7c7b\u75be\u75c5\u65b9\u9762\u7684\u6f5c\u529b\u3002\u7814\u7a76\u6210\u679c\u4ee5\u9898\u4e3a<strong>\u201cEndothelial siRNA delivery in nonhuman primates using ionizable low\u2013molecular weight polymeric nanoparticles\u201d<\/strong>\u53d1\u5e03\u5728\u56fd\u9645\u8457\u540d\u671f\u520a<strong>Sci. Adv.<\/strong>\u4e0a\u3002<\/p>\n<p><strong>\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/strong><\/p>\n<p><strong>\u56fe\u4e00\u30017C1\u7684\u63cf\u8ff0\u53ca\u5728\u975e\u4eba\u7075\u957f\u7c7b\u52a8\u7269\u4f53\u5185\u7597\u6548<\/strong><\/p>\n<p><strong><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-144561\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2018\/07\/1-2.jpg\" alt=\"\" width=\"600\" height=\"1014\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2018\/07\/1-2.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2018\/07\/1-2-178x300.jpg 178w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/strong><\/p>\n<p>\u00a0\uff08a\uff09 7C1 siRNA\u7eb3\u7c73\u9897\u7c92\u7684\u793a\u610f\u56fe\uff1b<\/p>\n<p>\uff08b\uff09 \u5b9a\u91cf\u57fa\u56e02.0\u6cd5\u6d4b\u5b9a7C1-Tie2 siRNA\u7eb3\u7c73\u9897\u7c92\u9759\u8109\u6ce8\u5c04\u540e48 h\uff0c\u975e\u4eba\u7c7b\u7075\u957f\u7c7b\u7ec4\u7ec7Tie2 mRNA\u6c34\u5e73\u4e3a1 mg\/kg\uff1b<\/p>\n<p>\uff08c\uff09 CD31<sup>+<\/sup>(\u5185\u76ae\u7ec6\u80de)\u4e2dTie2<sup>+<\/sup>\u7684\u767e\u5206\u6bd4\u3002<\/p>\n<p><strong>\u56fe\u4e8c\u30017C1 siRNA\u6cbb\u7597\u524d\u540e\u7684\u975e\u4eba\u7c7b\u7075\u957f\u7c7b\u52a8\u7269\u8840\u6db2\u5316\u5b66\u548c\u809d\u529f\u80fd\u6570\u636e\u00a0<\/strong><\/p>\n<p><strong><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-144562\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2018\/07\/2-2.jpg\" alt=\"\" width=\"600\" height=\"332\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2018\/07\/2-2.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2018\/07\/2-2-300x166.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/strong><\/p>\n<p><strong>\u56fe\u4e09\u30017C1 siRNA\u7eb3\u7c73\u9897\u7c92\u5904\u7406\u540e\u975e\u4eba\u7c7b\u7075\u957f\u7c7b\u52a8\u7269\u8840\u6e05\u4e2d\u708e\u75c7\u7ec6\u80de\u56e0\u5b50\u6d53\u5ea6\u7684\u53d8\u5316\u00a0<\/strong><\/p>\n<p><strong><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-144563\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2018\/07\/3-2.jpg\" alt=\"\" width=\"600\" height=\"800\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2018\/07\/3-2.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2018\/07\/3-2-225x300.jpg 225w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/strong><\/p>\n<p>\uff08a\uff09 Tie2 siRNA\u6cbb\u7597\u540e\uff0c\u4e0d\u540c\u65f6\u95f4\u70b9\u7684\u60c5\u51b5\uff1b<\/p>\n<p>\uff08b\uff09 \u7528Luc\u63a7\u5236siRNA\u6cbb\u7597\u3002<\/p>\n<p><strong>\u56fe\u56db\u3001\u975e\u4eba\u7c7b\u7075\u957f\u7c7b\u7ec4\u7ec7\u4e2d\u7684\u4e00\u4e2a\u5b50\u96c6\u7684\u4ee3\u8868\u6027\u7684\u540e\u6cbb\u7597\u7ec4\u7ec7\u5b66\u56fe\u50cf\u00a0<\/strong><\/p>\n<p><strong><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-144564\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2018\/07\/4-2.jpg\" alt=\"\" width=\"600\" height=\"1097\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2018\/07\/4-2.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2018\/07\/4-2-164x300.jpg 164w, \/\/m.iemloyee.com\/wp-content\/uploads\/2018\/07\/4-2-560x1024.jpg 560w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/strong><br \/>\n <strong>\u3010\u5c0f\u7ed3\u3011<\/strong><\/p>\n<p>\u5728\u975e\u4eba\u7c7b\u7075\u957f\u7c7b\u52a8\u7269\u7684\u65e9\u671f\u7684\u8f6c\u5316\u7814\u7a76\u8868\u660e\uff0c\u7531\u53ef\u7535\u79bb\u7684\u4f4e\u5206\u5b50\u91cf\u805a\u5408\u72697C1\u548csiRNAs\u5236\u6210\u7684\u7eb3\u7c73\u9897\u7c92\u53ef\u4ee5\u8bf1\u5bfc\u591a\u5668\u5b98\u5185\u76ae\u4e2d\u7684RNAi\u3002\u6700\u5927\u6570\u91cf\u7684\u57fa\u56e0\u6572\u9664\u5728\u80ba\u548c\u5fc3\u810f\u88ab\u89c2\u5bdf\u5230\uff0c\u8fd9\u4e0e\u4ee5\u524d\u7684\u556e\u9f7f\u52a8\u7269\u7814\u7a76\u5f88\u4e00\u81f4\u3002\u6b64\u5916\uff0c\u5728\u89c6\u7f51\u819c\u3001\u80be\u810f\u3001\u80f0\u817a\u548c\u9aa8\u9abc\u7684\u8109\u7ba1\u7cfb\u7edf\u4e2d\u53d1\u73b0\u4e86\u6709\u6548\u7684\u57fa\u56e0\u6572\u9664\uff0c\u6709\u53ef\u80fd\u4e3a\u8fd9\u4e9b\u5668\u5b98\u548c\u7ec4\u7ec7\u521b\u9020\u65b0\u7684\u4ee5\u8840\u7ba1\u4e3a\u4e2d\u5fc3\u7684\u3001\u57fa\u4e8eRNA\u7684\u6cbb\u7597\u65b9\u6cd5\u3002\u6b64\u5916\uff0c\u57281 mg\/kg\u7684\u5242\u91cf\u4e0b\uff0c\u5728\u809d\u810f\u5185\u76ae\u7ec6\u80de\u4e2d\u6ca1\u6709\u89c2\u5bdf\u5230\u6709\u7edf\u8ba1\u5b66\u610f\u4e49\u7684\u6572\u9664\uff0c\u6709\u52a9\u4e8e\u907f\u514d\u809d\u76f8\u5173\u7684\u9776\u5916\u6548\u5e94\u3002\u6b64\u5916\uff0c\u5728\u8fd9\u4e9b\u7814\u7a76\u8fc7\u7a0b\u4e2d\u6ca1\u6709\u53d1\u73b0\u6025\u6027\u6bd2\u6027\u7684\u8ff9\u8c61,\u4e14\u6b64\u6b21\u7814\u7a76\u63d0\u4f9b\u4e86\u65b0\u7684\u8bc1\u636e\uff0c\u8bc1\u660e\u4f7f\u7528\u5168\u8eab\u7528\u836f\u7684siRNA\u7eb3\u7c73\u9897\u7c92\uff0c\u5728\u5927\u578b\u975e\u4eba\u7075\u957f\u7c7b\u52a8\u7269\u4e2d\uff0c\u6709\u5185\u76ae\u57fa\u56e0\u6c89\u9ed8\u73b0\u8c61\u3002<\/p>\n<p><strong>\u6587\u732e\u94fe\u63a5\uff1a<\/strong><a href=\"http:\/\/advances.sciencemag.org\/content\/4\/6\/eaar8409\" target=\"_blank\" rel=\"noopener\">Endothelial siRNA delivery in nonhuman primates using ionizable low\u2013molecular weight polymeric nanoparticles<\/a>\uff08Sci. Adv.,<strong> 2018<\/strong>, DOI: 10.1126\/sciadv.aar8409\uff09<\/p>\n<p><strong>\u672c\u6587\u7531\u6750\u6599\u4eba\u751f\u7269\u6750\u6599\u7ec4\u5c0f\u80d6\u7eb8\u7f16\u8bd1\u3002<\/strong><\/p>\n<p>\u6750\u6599\u725b\u7f51\u4e13\u6ce8\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u53ca\u884c\u4e1a\u8fdb\u5c55\uff0c\u8fd9\u91cc\u6c47\u96c6\u4e86\u5404\u5927\u9ad8\u6821\u7855\u535a\u751f\u3001\u4e00\u7ebf\u79d1\u7814\u4eba\u5458\u4ee5\u53ca\u884c\u4e1a\u4ece\u4e1a\u8005\uff0c\u5982\u679c\u60a8\u5bf9\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u8fdb\u5c55\uff0c\u89e3\u8bfb\u9ad8\u6c34\u5e73\u6587\u7ae0\u6216\u662f\u8bc4\u8ff0\u884c\u4e1a\u6709\u5174\u8da3\uff0c<a href=\"\/\/m.iemloyee.com\/131937.html\" target=\"_blank\" rel=\"noopener\">\u70b9\u6211\u52a0\u5165\u6750\u6599\u4eba\u7f16\u8f91\u90e8<\/a>\u3002<\/p>\n<p>\u6b22\u8fce\u5927\u5bb6\u5230\u6750\u6599\u4eba\u5ba3\u4f20\u79d1\u6280\u6210\u679c\u5e76\u5bf9\u6587\u732e\u8fdb\u884c\u6df1\u5165\u89e3\u8bfb\uff0c\u6295\u7a3f\u90ae\u7bb1tougao@cailiaoren.com.<\/p>\n<p>\u6295\u7a3f\u4ee5\u53ca\u5185\u5bb9\u5408\u4f5c\u53ef\u52a0\u7f16\u8f91\u5fae\u4fe1\uff1acailiaokefu.<\/p>\n<p>\u6750\u6599\u6d4b\u8bd5\uff0c\u6570\u636e\u5206\u6790\uff0c\u4e0a<a href=\"http:\/\/www.ceshigu.com\/\" target=\"_blank\" rel=\"noopener\">\u6d4b\u8bd5\u8c37<\/a>\uff01<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u3010\u5f15\u8a00\u3011 \u5065\u5eb7\u7684\u5185\u76ae\u7ec6\u80de\u5bf9\u4e8e\u91cd\u8981\u7684\u751f\u7406\u529f\u80fd\u662f\u5fc5\u4e0d\u53ef\u5c11\u7684\uff0c\u5305\u62ec\u6b62\u8840\u3001\u514d\u75ab\u53cd\u5e94\u3001\u7ec4\u7ec7\u751f\u957f\u548c\u8840\u7ba1\u8c03\u8282\u3002\u7136\u800c\uff0c\u529f\u80fd\u4e0d\u5168\u7684\u5185\u76ae\u662f\u8bb8\u591a\u75be\u75c5\u7684\u6807\u5fd7\uff0c\u5305\u62ec\u5468\u56f4\u8840\u7ba1\u75be\u75c5\u3001\u4e2d\u98ce\u3001\u9ad8&#8230;<\/p>\n","protected":false},"author":7770,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[215,2],"tags":[3371,3372],"class_list":["post-144560","post","type-post","status-publish","format-standard","hentry","category-jiedu","category-keji","tag-7c1","tag-sirna"],"_links":{"self":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/144560"}],"collection":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/users\/7770"}],"replies":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=144560"}],"version-history":[{"count":5,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/144560\/revisions"}],"predecessor-version":[{"id":144621,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/144560\/revisions\/144621"}],"wp:attachment":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=144560"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=144560"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=144560"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}