{"id":140976,"date":"2018-06-08T06:30:21","date_gmt":"2018-06-07T22:30:21","guid":{"rendered":"\/\/m.iemloyee.com\/?p=140976"},"modified":"2018-06-07T18:07:46","modified_gmt":"2018-06-07T10:07:46","slug":"%e6%b9%96%e5%8d%97%e5%a4%a7%e5%ad%a6%e8%b0%ad%e8%94%9a%e6%b3%93%e6%95%99%e6%8e%88j-am-chem-soc-%e8%b6%85%e5%88%86%e5%ad%90%e7%bb%84%e8%a3%85%e7%9a%84%e5%9c%86%e5%bd%a2%e4%ba%8c%e9%98%b6%e9%80%82","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=140976","title":{"rendered":"\u6e56\u5357\u5927\u5b66\u8c2d\u851a\u6cd3\u6559\u6388J. Am. Chem. Soc.: \u8d85\u5206\u5b50\u7ec4\u88c5\u7684\u5706\u5f62\u4e8c\u9636\u9002\u4f53\u7528\u4e8e\u589e\u5f3a\u529f\u80fd\u86cb\u767d\u8d28\u7684\u4f20\u9012"},"content":{"rendered":"

\u3010\u5f15\u8a00<\/b><\/strong>\u3011<\/p>\n

\u9002\u4f53\uff0c\u4e5f\u88ab\u79f0\u4e3a\u5316\u5b66\u6297\u4f53\uff0c\u662f\u4e00\u7c7b\u53ef\u4ee5\u9ad8\u5ea6\u9776\u5411\u751f\u7269\u5927\u5206\u5b50\u548c\u7ec6\u80de\u7684\u5be1\u805a\u6838\u82f7\u9178\u3002\u9002\u4f53\u51ed\u501f\u5176\u5353\u8d8a\u7684\u5206\u5b50\u8bc6\u522b\u6027\u8d28\u5728\u751f\u7269\u533b\u7528\u9886\u57df\u6709\u7740\u5e7f\u6cdb\u7684\u5e94\u7528\uff0c\u6bd4\u5982\uff0c\u4e0e\u5316\u5b66\u836f\u7269\u8fde\u63a5\u800c\u5b9e\u73b0\u9776\u5411\u764c\u75c7\u6cbb\u7597\u3002\u7136\u800c\uff0c\u9002\u4f53-\u836f\u7269\u5076\u8054\u7269\u7684\u5b9e\u9645\u5e94\u7528\u8981\u6c42\u5b9a\u5236\u5408\u6210\u9002\u4f53\u5076\u8054\u7269\u3002\u8fd9\u4e9b\u5076\u8054\u7269\u4e0d\u4ec5\u53d7\u5230\u57fa\u7840\u5408\u6210\u7684\u9650\u5236\uff0c\u540c\u65f6\u4e5f\u6709\u53ef\u80fd\u5f71\u54cd\u5230\u9002\u4f53\u7684\u9776\u5411\u80fd\u529b\u3002\u56e0\u6b64\uff0c\u65b0\u578b\u7684\u5316\u5b66\u65b9\u6cd5\u6765\u8c03\u6574\u9002\u4f53\u7ed3\u6784\uff0c\u63d0\u9ad8\u9002\u4f53\u7684\u7a33\u5b9a\u6027\u548c\u8bc6\u522b\u80fd\u529b\u662f\u6025\u9700\u7684\u3002<\/p>\n

\u3010\u6210\u679c\u7b80\u4ecb<\/b><\/strong>\u3011<\/p>\n

\u8fd1\u65e5\uff0c\u6e56\u5357\u5927\u5b66<\/b><\/strong>\u7684\u8c2d\u851a\u6cd3\u6559\u6388<\/b><\/strong>\u56e2\u961f\u8bbe\u8ba1\u4e86\u4e00\u79cd\u03b2-\u73af\u7cca\u7cbe\u4fee\u9970\u7684\u5706\u5f62\u4e8c\u9636\u9002\u4f53(cb-apt-\u03b2CD)\uff0c\u5176\u53ef\u4ee5\u901a\u8fc7\u4e3b\u5ba2\u4f53\u5316\u5b66\u7684\u8d85\u5206\u5b50\u4f5c\u7528\u6765\u5b9e\u73b0\u9776\u5411\u5206\u5b50\u5185\u6cbb\u7597\u3002\u8fd9\u79cd\u8d85\u5206\u5b50\u7ec4\u88c5\u4f53\u548c\u5355\u4e00\u7684\u9002\u4f53\u76f8\u6bd4\uff0c\u5177\u6709\u8f83\u9ad8\u7684\u8840\u6e05\u7a33\u5b9a\u6027\u548c\u589e\u5f3a\u7684\u7ec6\u80de\u5185\u4f20\u9012\u6548\u7387\u3002\u805a\u96c6\u4f53cb-apt-\u03b2CD\u53ef\u4ee5\u9776\u5411\u5411\u7ec6\u80de\u4f20\u9012\u7eff\u8272\u8367\u5149\u86cb\u767d\uff0c\u4f20\u9012\u6548\u7387\u9ad8\u8fbe80%\u3002\u8fd9\u79cd\u5c06\u03b2CD\u8fde\u63a5\u5230cb-apt\uff0c\u5e76\u4e14\u901a\u8fc7\u8d85\u5206\u5b50\u5316\u5b66\u6765\u8c03\u8282cb-apt-\u03b2CD\u7684\u7b56\u7565\uff0c\u4e3a\u540e\u7eed\u6269\u5c55\u548c\u4e30\u5bcc\u9002\u4f53\u7684\u529f\u80fd\u63d0\u4f9b\u4e86\u4e00\u4e2a\u5e73\u53f0\u3002\u8be5\u6210\u679c\u4ee5\u9898\u4e3a”Supramolecularly Engineered Circular Bivalent Aptamer for Enhanced Functional Protein Delivery<\/b><\/strong>“\u53d1\u8868\u5728J. Am. Chem. Soc.<\/b><\/strong>\u4e0a\u3002<\/p>\n

\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/b><\/strong><\/p>\n

\u56fe<\/b><\/strong>1<\/b><\/strong>.<\/b><\/strong>\u00a0<\/b><\/strong>\u03b2CD\u4fee\u9970\u7684\u5355\u5206\u5b50\u9002\u4f53\u7684\u5408\u6210\u53ca\u8868\u5f81<\/b><\/strong><\/p>\n

\"\"<\/p>\n

(a).\u901a\u8fc7\u70b9\u51fb\u53cd\u5e94\u5408\u6210\u03b2CD\u4fee\u9970\u7684\u5355\u5206\u5b50\u9002\u4f53<\/p>\n

(b).RP-HPLC\u8ffd\u8e2a\u6c28\u57fa\uff0cDBCO\u548c\u03b2CD\u4fee\u9970\u7684sgc8\u9002\u4f53<\/p>\n

(c).cb-apt-\u03b2CD\u7684PAGE\u5206\u6790<\/p>\n

\u56fe<\/b><\/strong>2<\/b><\/strong>. <\/b><\/strong>GFP-AdA\u7684\u7ed3\u6784\u548c\u5149\u8c31\u6027\u8d28<\/b><\/strong><\/p>\n

\"\"<\/b><\/strong><\/p>\n

(a).GFP-AdA\u7684\u5316\u5b66\u7ed3\u6784<\/p>\n

(b).GFP-AdA, GFP-AdA\u548cTMR-apt, GFP-AdA\u548cTMR-cb-apt-\u03b2CD\u5728PBS\u4e2d\u7684\u8367\u5149\u5149\u8c31<\/p>\n

(c).0-1.5 \u03bcM TMR-apt-\u03b2CD\u6ef4\u5b9a\u5230GFP-AdA\u4e2d\u7684\u8367\u5149\u5149\u8c31<\/p>\n

\u56fe<\/b><\/strong>3<\/b><\/strong>. <\/b><\/strong>Cb-apt-\u03b2CD\u8c03\u8282\u7684\u589e\u5f3aGFP\u7ec6\u80de\u4f20\u9012<\/b><\/strong><\/p>\n

\"\"<\/b><\/strong><\/p>\n

(a).GFP-AdA\u4e0ecb-apt-\u03b2CD\u7684\u914d\u5408\u7269\u4ee5\u53ca\u5355\u5206\u5b50apt-\u03b2CD\u7684\u7ec6\u80de\u6210\u50cf\u56fe<\/p>\n

(b).PBS, GFP-AdA, \u5355\u5206\u5b50\u9002\u4f53\/ GFP-AdA\u548c\u00a0cb-apt\/GFP-AdA\u5904\u7406\u540e\u7684\u7ec6\u80de\u7684\u6d41\u5f0f\u7ec6\u80de\u4eea\u6570\u636e<\/p>\n

\u56fe<\/b><\/strong>4<\/b><\/strong>. <\/b><\/strong>\u4e0d\u540capt-\u03b2CD-\u7682\u8349\u7d20\u914d\u5408\u7269\u5904\u7406\u8fc7\u7684\u7ec6\u80de\u7684\u5b58\u6d3b\u7387<\/b><\/strong><\/p>\n

\"\"<\/b><\/strong><\/p>\n

\u3010\u5c0f\u7ed3\u3011<\/b><\/strong><\/p>\n

\u5728\u8fd9\u4e2a\u5de5\u4f5c\u4e2d\uff0c\u4f5c\u8005\u8bbe\u8ba1\u4e86\u4e00\u79cd\u03b2-\u73af\u7cca\u7cbe\u4fee\u9970\u7684\u5706\u5f62\u4e8c\u9636\u9002\u4f53(cb-apt-\u03b2CD)\uff0c\u5176\u53ef\u4ee5\u901a\u8fc7\u4e3b\u5ba2\u4f53\u5316\u5b66\u7684\u8d85\u5206\u5b50\u4f5c\u7528\u6765\u5b9e\u73b0\u9776\u5411\u5206\u5b50\u5185\u6cbb\u7597\u3002\u8fd9\u79cd\u8d85\u5206\u5b50\u7ec4\u88c5\u4f53\u548c\u5355\u4e00\u7684\u9002\u4f53\u76f8\u6bd4\uff0c\u5177\u6709\u8f83\u9ad8\u7684\u8840\u6e05\u7a33\u5b9a\u6027\u548c\u589e\u5f3a\u7684\u7ec6\u80de\u5185\u4f20\u9012\u6548\u7387\u3002\u805a\u96c6\u4f53cb-apt-\u03b2CD\u53ef\u4ee5\u9776\u5411\u5411\u7ec6\u80de\u4f20\u9012\u7eff\u8272\u8367\u5149\u86cb\u767d\uff0c\u4f20\u9012\u6548\u7387\u9ad8\u8fbe80%\u3002\u8fd9\u79cd\u5c06\u03b2CD\u8fde\u63a5\u5230cb-apt\uff0c\u5e76\u4e14\u901a\u8fc7\u8d85\u5206\u5b50\u5316\u5b66\u6765\u8c03\u8282cb-apt-\u03b2CD\u7684\u7b56\u7565\uff0c\u4e3a\u540e\u7eed\u6269\u5c55\u548c\u4e30\u5bcc\u9002\u4f53\u7684\u529f\u80fd\u63d0\u4f9b\u4e86\u4e00\u4e2a\u5e73\u53f0\u3002<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1a<\/strong>Supramolecularly Engineered Circular Bivalent Aptamer for Enhanced Functional Protein Delivery<\/a>\u00a0(J. Am. Chem. Soc., 2018, DOI: 10. 1021\/jacs.8b03442)<\/p>\n

\u672c\u6587\u7531\u6750\u6599\u4eba\u5b66\u672f\u7ec4gaxy\u4f9b\u7a3f\uff0c\u6750\u6599\u725b\u6574\u7406\u7f16\u8f91\u3002 \u00a0<\/p>\n

\u6750\u6599\u725b\u7f51\u4e13\u6ce8\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u53ca\u884c\u4e1a\u8fdb\u5c55\uff0c\u8fd9\u91cc\u6c47\u96c6\u4e86\u5404\u5927\u9ad8\u6821\u7855\u535a\u751f\u3001\u4e00\u7ebf\u79d1\u7814\u4eba\u5458\u4ee5\u53ca\u884c\u4e1a\u4ece\u4e1a\u8005\uff0c\u5982\u679c\u60a8\u5bf9\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u8fdb\u5c55\uff0c\u89e3\u8bfb\u9ad8\u6c34\u5e73\u6587\u7ae0\u6216\u662f\u8bc4\u8ff0\u884c\u4e1a\u6709\u5174\u8da3\uff0c\u70b9\u6211\u52a0\u5165\u7f16\u8f91\u90e8\u5927\u5bb6\u5ead<\/a>\u3002 \u00a0<\/p>\n

\u6b22\u8fce\u5927\u5bb6\u5230\u6750\u6599\u4eba\u5ba3\u4f20\u79d1\u6280\u6210\u679c\u5e76\u5bf9\u6587\u732e\u8fdb\u884c\u6df1\u5165\u89e3\u8bfb\uff0c\u6295\u7a3f\u90ae\u7bb1tougao@cailiaoren.com\u3002<\/p>\n

\u6295\u7a3f\u4ee5\u53ca\u5185\u5bb9\u5408\u4f5c\u53ef\u52a0\u7f16\u8f91\u5fae\u4fe1\uff1acailiaokefu\uff0c\u6211\u4eec\u4f1a\u9080\u8bf7\u5404\u4f4d\u8001\u5e08\u52a0\u5165\u4e13\u5bb6\u7fa4\u3002 \u00a0<\/p>\n

\u6750\u6599\u6d4b\u8bd5\uff0c\u6570\u636e\u5206\u6790\uff0c\u4e0a\u6d4b\u8bd5\u8c37<\/a>\uff01<\/b><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u3010\u5f15\u8a00\u3011 \u9002\u4f53\uff0c\u4e5f\u88ab\u79f0\u4e3a\u5316\u5b66\u6297\u4f53\uff0c\u662f\u4e00\u7c7b\u53ef\u4ee5\u9ad8\u5ea6\u9776\u5411\u751f\u7269\u5927\u5206\u5b50\u548c\u7ec6\u80de\u7684\u5be1\u805a\u6838\u82f7\u9178\u3002\u9002\u4f53\u51ed\u501f\u5176\u5353\u8d8a\u7684\u5206\u5b50\u8bc6\u522b\u6027\u8d28\u5728\u751f\u7269\u533b\u7528\u9886\u57df\u6709\u7740\u5e7f\u6cdb\u7684\u5e94\u7528\uff0c\u6bd4\u5982\uff0c\u4e0e\u5316\u5b66\u836f\u7269\u8fde\u63a5…<\/p>\n","protected":false},"author":7336,"featured_media":140977,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[215],"tags":[75,33],"class_list":["post-140976","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-jiedu","tag-biomaterial","tag-nano"],"_links":{"self":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/140976"}],"collection":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/users\/7336"}],"replies":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=140976"}],"version-history":[{"count":2,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/140976\/revisions"}],"predecessor-version":[{"id":141746,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/140976\/revisions\/141746"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/media\/140977"}],"wp:attachment":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=140976"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=140976"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=140976"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}