{"id":138818,"date":"2018-05-21T14:00:01","date_gmt":"2018-05-21T06:00:01","guid":{"rendered":"\/\/m.iemloyee.com\/?p=138818"},"modified":"2018-05-20T21:30:48","modified_gmt":"2018-05-20T13:30:48","slug":"%e5%9b%bd%e5%ae%b6%e7%ba%b3%e7%b1%b3%e4%b8%ad%e5%bf%83%e4%b8%81%e5%ae%9d%e5%85%a8%e6%95%99%e6%8e%88nano-letters-%e4%b8%80%e7%a7%8d%e5%9f%ba%e4%ba%8edna%e7%9a%84%e7%ba%b3%e7%b1%b3%e8%bd%bd%e4%bd%93","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=138818","title":{"rendered":"\u56fd\u5bb6\u7eb3\u7c73\u4e2d\u5fc3\u4e01\u5b9d\u5168\u6559\u6388Nano Letters: \u4e00\u79cd\u57fa\u4e8eDNA\u7684\u7eb3\u7c73\u8f7d\u4f53\u5b9e\u73b0\u6709\u6548\u7684\u57fa\u56e0\u4f20\u9012\u548c\u764c\u75c7\u6cbb\u7597"},"content":{"rendered":"

\u3010\u5f15\u8a00<\/b><\/strong>\u3011<\/p>\n

\u57fa\u56e0\u7597\u6cd5\u662f\u4e00\u79cd\u80fd\u591f\u6cbb\u7597\u8bb8\u591a\u57fa\u56e0\u76f8\u5173\u75be\u75c5\u7684\u6cbb\u7597\u65b9\u6cd5\u3002\u57fa\u4e8e\u75c5\u6bd2\u8f7d\u4f53\u7684\u57fa\u56e0\u6cbb\u7597\u836f\u7269\uff0c\u6bd4\u5982\u6cbb\u7597\u8102\u86cb\u767d\u8102\u9176\u7f3a\u5931\u7684\u963f\u5229\u6cfc\u91d1\u548c\u6cbb\u7597\u817a\u82f7\u8131\u6c28\u9176\u7f3a\u4e4f\u6027\u91cd\u5ea6\u8054\u5408\u514d\u75ab\u7f3a\u9677\u75c7\u7684strimvelis\u90fd\u5df2\u7ecf\u88ab\u6279\u51c6\u4e0a\u5e02\u3002\u53e6\u5916\u4e00\u79cd\u6709\u6548\u7684\u764c\u75c7\u57fa\u56e0\u7597\u6cd5\u662f\u589e\u52a0\u80bf\u7624\u6291\u5236\u57fa\u56e0\u7684\u8868\u8fbe\uff0c\u56e0\u6b64\uff0cp53\u4f5c\u4e3a\u4e00\u79cd\u91cd\u8981\u7684\u80bf\u7624\u6291\u5236\u57fa\u56e0\u5728\u764c\u75c7\u6cbb\u7597\u4e2d\u5341\u5206\u5f15\u4eba\u77a9\u76ee\u3002\u76ee\u524d\uff0c\u5f88\u591a\u7684\u7814\u7a76\u7ed3\u679c\u53d1\u73b0p53\u7684\u9ad8\u8868\u8fbe\u53ef\u4ee5\u63d0\u9ad8\u591a\u79cd\u6297\u836f\u80bf\u7624\u5bf9\u836f\u7269\u6cbb\u7597\u7684\u7075\u654f\u5ea6\u3002\u57fa\u4e8e\u6b64\uff0c\u5f88\u591a\u7814\u7a76\u90fd\u5c06\u5173\u6ce8\u70b9\u653e\u5728\u4e86p53\u57fa\u56e0\u6cbb\u7597\u548c\u5316\u5b66\u6cbb\u7597\u76f8\u7ed3\u5408\u7684\u65b9\u6cd5\u6765\u63d0\u9ad8\u6297\u80bf\u7624\u6548\u679c\u3002<\/p>\n

\u3010\u6210\u679c\u7b80\u4ecb<\/b><\/strong>\u3011<\/p>\n

\u8fd1\u65e5\uff0c\u56fd\u5bb6\u7eb3\u7c73\u4e2d\u5fc3<\/b><\/strong>\u7684\u4e01\u5b9d\u5168\u6559\u6388<\/b><\/strong>\u8bfe\u9898\u7ec4\u62a5\u9053\u4e86\u4e00\u79cd\u7b80\u5355\u4e14\u901a\u7528\u7684\u7528\u6765\u6784\u5efa\u57fa\u4e8eDNA\u7eb3\u7c73\u7ed3\u6784\u7684\u836f\u7269\u4f20\u9012\u7cfb\u7edf\u3002\u8fd9\u4e2a\u4f53\u7cfb\u5305\u542b\u7ebf\u6027\u7684\u80bf\u7624\u6cbb\u7597\u57fa\u56e0p53\u548c\u5316\u5b66\u6cbb\u7597\u836f\u7269\u963f\u9709\u7d20\uff0c\u53ef\u5b9e\u73b0\u591a\u79cd\u6297\u836f\u80bf\u7624\u7684\u8054\u5408\u6cbb\u7597\u3002\u8fd9\u79cd\u7ed3\u6784\u65b0\u9896\u7684\u8f7d\u836f\u4f53\u7cfb\u53ef\u5b9e\u73b0\u6709\u6548\u7684\u57fa\u56e0\u4f20\u9012\uff0c\u5e76\u5728\u4f53\u5916\u548c\u4f53\u5185\u5b9e\u9a8c\u4e2d\u5747\u53d6\u5f97\u4e86\u6709\u6548\u7684\u80bf\u7624\u6291\u5236\u6548\u679c\u3002\u8fd9\u79cd\u8f7d\u836f\u4f53\u7cfb\u4e3a\u57fa\u56e0\u6cbb\u7597\u7684\u53d1\u5c55\u63d0\u4f9b\u4e86\u65b0\u7684\u60f3\u6cd5\u3002\u8be5\u6210\u679c\u4ee5\u9898\u4e3a”A DNA-Based Nanocarrier for Efficient Gene Delivery and Combined Cancer Therapy<\/b><\/strong>“\u53d1\u8868\u5728Nano Letters<\/b><\/strong>\u4e0a\u3002<\/p>\n

\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/b><\/strong><\/p>\n

\u56fe<\/b><\/strong>1<\/b><\/strong>.<\/b><\/strong>\u00a0<\/b><\/strong>\u56fe\u793a\u57fa\u4e8eDNA\u7eb3\u7c73\u7ed3\u6784\u7684\u8f7d\u836f\u4f53\u7cfb\u901a\u8fc7\u7ed3\u5408\u57fa\u56e0\u7597\u6cd5\u548c\u5316\u5b66\u7597\u6cd5\u5728\u6297\u836f\u80bf\u7624\u6cbb\u7597\u4e2d\u7684\u5e94\u7528<\/b><\/strong><\/p>\n

\"\"<\/b><\/strong><\/p>\n

\u56fe<\/b><\/strong>2<\/b><\/strong>. <\/b><\/strong>\u57fa\u4e8eDNA\u7684\u7eb3\u7c73\u8f7d\u4f53\u7684\u5236\u5907\u53ca\u8868\u5f81<\/b><\/strong><\/p>\n

\"\"<\/b><\/strong><\/p>\n

(a).\u51dd\u80f6\u7535\u6cf3\u5206\u6790\u56fe<\/p>\n

(b).\u65f6\u95f4\u4f9d\u8d56\u7684\u836f\u7269\u8d1f\u8f7d<\/p>\n

(c).AFM\u8868\u5f81<\/p>\n

\u56fe<\/b><\/strong>3<\/b><\/strong>. <\/b><\/strong>\u4ece\u7ec6\u80de\u6c34\u5e73\u5206\u6790\u57fa\u4e8eDNA\u7eb3\u7c73\u7ed3\u6784\u7684\u836f\u7269\u5171\u4f20\u9012\u4f53\u7cfb<\/b><\/strong><\/p>\n

\"\"<\/b><\/strong><\/p>\n

(a,b).DOX, TOD, TOP\u548cTODP\u57f9\u80b2MCF-7\u7ec6\u80de12\u5c0f\u65f6\u540e\u7684\u6fc0\u5149\u5171\u805a\u7126\u56fe\u50cf\u548c\u6d41\u5f0f\u7ec6\u80de\u4eea\u5206\u6790\uff1b<\/p>\n

(c).\u81ea\u7531\u7ebf\u6027EGFP\u57fa\u56e0\uff0clipo\u8f6c\u67d3\u7684\u7ebf\u6027EGFP\u57fa\u56e0\u548c\u4e09\u89d2DNA\u8d1f\u8f7d\u7684\u7ebf\u6027EGFP\u57fa\u56e0\u57f9\u80b2MCF-7\u7ec6\u80de48\u5c0f\u65f6\u540e\u7684\u6d41\u5f0f\u7ec6\u80de\u4eea\u5206\u6790\uff1b<\/p>\n

(d).PBS, TOD, TOP\u548cTODP\u57f9\u80b2MCF-7\u7ec6\u80de48\u5c0f\u65f6\u540e\u7684\u86cb\u767d\u5206\u6790\uff1b<\/p>\n

(e).TO, TOP, TOD\u548cTODP\u57f9\u80b2MCF-7\u7ec6\u80de48\u5c0f\u65f6\u540e\u7684\u7ec6\u80de\u5b58\u6d3b\u7387\uff1b<\/p>\n

\u56fe<\/b><\/strong>4<\/b><\/strong>. <\/b><\/strong>\u6240\u6784\u5efa\u7684\u836f\u7269\u4f20\u8f93\u4f53\u7cfb\u7684\u751f\u7269\u5206\u5e03\u548c\u514d\u75ab\u54cd\u5e94<\/b><\/strong><\/p>\n

\"\"<\/b><\/strong><\/p>\n

(a).Cy5.5\u6807\u8bb0\u7684\u5355\u94feDNA\uff0c\u65e0\u9002\u914d\u5b50\u7684TOP\u548c\u9002\u914d\u5b50\u7ec4\u88c5\u7684TOP\u5728\u542b\u6709\u80bf\u7624\u5c0f\u9f20\u4f53\u5185\u7684\u52a8\u6001\u5206\u5e03\uff1b<\/p>\n

(b).\u80bf\u7624\u4e0e\u4e3b\u8981\u5668\u5b98\u7684\u8367\u5149\u6210\u50cf\u548c\u5e73\u5747\u8367\u5149\u5f3a\u5ea6\uff1b<\/p>\n

(c).\u514d\u75ab\u7ec4\u7ec7\u5316\u5b66\u5206\u6790\uff1b<\/p>\n

(d).\u4e0d\u540c\u5904\u7406\u540e\u7684\u5c0f\u9f20\u7684\u514d\u75ab\u54cd\u5e94\uff1b<\/p>\n

\u56fe<\/b><\/strong>5<\/b><\/strong>. <\/b><\/strong>\u6d3b\u4f53\u5b9e\u9a8c<\/b><\/strong><\/p>\n

\"\"<\/p>\n

(a).\u7ecf\u8fc7\u4e0d\u540c\u5904\u7406\u540e\uff0c\u80bf\u7624\u7684\u4f53\u79ef\u53d8\u5316\uff1b<\/p>\n

(b).\u7ecf\u8fc7\u4e0d\u540c\u5904\u7406\u540e\uff0c\u79bb\u4f53\u7684\u80bf\u7624\u7167\u7247\uff1b<\/p>\n

(c).\u80bf\u7624\u7ec4\u7ec7\u5185p53\u7684mRNA\u6c34\u5e73\uff1b<\/p>\n

(d).\u7ecf\u8fc7\u4e0d\u540c\u5904\u7406\u540e\uff0c\u5c0f\u9f20\u4f53\u91cd\u53d8\u5316\uff1b<\/p>\n

(e).\u7ecf\u8fc7\u4e0d\u540c\u5904\u7406\u540e\uff0c\u5c0f\u9f20\u5668\u5b98\u7684\u5207\u7247\u56fe\uff1b<\/p>\n

\u3010\u5c0f\u7ed3\u3011<\/b><\/strong><\/p>\n

\u5728\u8fd9\u4e2a\u5de5\u4f5c\u4e2d\uff0c\u4f5c\u8005\u4ecb\u7ecd\u4e86\u4e00\u79cd\u7b80\u5355\u4e14\u901a\u7528\u7684\u7528\u6765\u6784\u5efa\u57fa\u4e8eDNA\u7eb3\u7c73\u7ed3\u6784\u7684\u836f\u7269\u4f20\u9012\u7cfb\u7edf\u3002\u8fd9\u4e2a\u4f53\u7cfb\u5305\u542b\u7ebf\u6027\u7684\u80bf\u7624\u6cbb\u7597\u57fa\u56e0p53\u548c\u5316\u5b66\u6cbb\u7597\u836f\u7269\u963f\u9709\u7d20\uff0c\u53ef\u5b9e\u73b0\u591a\u79cd\u6297\u836f\u80bf\u7624\u7684\u8054\u5408\u6cbb\u7597\u3002\u8fd9\u79cd\u7ed3\u6784\u65b0\u9896\u7684\u8f7d\u836f\u4f53\u7cfb\u53ef\u5b9e\u73b0\u6709\u6548\u7684\u57fa\u56e0\u4f20\u9012\uff0c\u5e76\u5728\u4f53\u5916\u548c\u4f53\u5185\u5b9e\u9a8c\u4e2d\u5747\u53d6\u5f97\u4e86\u6709\u6548\u7684\u80bf\u7624\u6291\u5236\u6548\u679c\u3002<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1a<\/b><\/strong>A DNA-Based Nanocarrier for Efficient Gene Delivery and Combined Cancer Therapy<\/a>(Nano Lett., 2018, DOI: 10. 1021\/acs.nanolett.7b04812)<\/p>\n

\u3010\u76f8\u5173\u4ecb\u7ecd\u3011<\/strong><\/p>\n

Nano Letters<\/b><\/strong>:<\/b><\/strong>\u00a0<\/b><\/strong>\u4e00\u79cd\u57fa\u4e8e<\/b><\/strong>DNA<\/b><\/strong>\u7684\u7eb3\u7c73\u8f7d\u4f53\u5b9e\u73b0\u6709\u6548\u7684\u57fa\u56e0\u4f20\u9012\u548c\u764c\u75c7\u6cbb\u7597<\/b><\/strong><\/p>\n

\u4f5c\u8005\uff1a\u5218\u5efa\u5175\uff0c\u5b8b\u7433\u7433\uff0c\u5218\u5c11\u5229\uff0c\u848b\u4e54\uff0c\u5218\u6e05\uff0c\u674e\u5a1c\uff0c\u738b\u632f\u521a\uff0c\u4e01\u5b9d\u5168<\/b><\/strong><\/p>\n

2012\u5e74\u4ee5\u6765\uff0c\u56fd\u5bb6\u7eb3\u7c73\u79d1\u5b66\u4e2d\u5fc3\u7684\u4e01\u5b9d\u5168\u8bfe\u9898\u7ec4\u4ee5\u81ea\u7ec4\u88c5\u7684\u591a\u529f\u80fd\u6838\u9178\u7eb3\u7c73\u7ed3\u6784\u4e3a\u57fa\u7840\uff0c\u6784\u5efa\u4e86\u4e00\u7cfb\u5217\u836f\u7269\u4f20\u9012\u8f7d\u4f53\u3002\u6240\u4f20\u9012\u7684\u836f\u7269\u7ec4\u5206\u6d89\u53ca\u5230\u5316\u7597\u5c0f\u5206\u5b50\u836f\u7269\uff08J. Am. Chem. Soc. 2012, 134, 13396; ACS Nano 2014, 8, 6633\uff09\uff0c\u5149\u70ed\u6cbb\u7597\u7eb3\u7c73\u9897\u7c92\u836f\u7269\uff08Small 2015, 11, 5134; Adv. Mater. 2016, 28, 10000\uff09\uff0c\u6838\u9178\u514d\u75ab\u836f\u7269\uff08ACS Appl. Mater. Interfaces 2017, 9, 20324\uff09\uff0c\u529f\u80fd\u6027\u86cb\u767d\u7c7b\u836f\u7269\uff08Nat. Biotechnol. 2018, 36, 258\uff09\u3002\u8be5\u7c7b\u591a\u529f\u80fd\u6838\u9178\u7eb3\u7c73\u836f\u7269\u4f20\u9012\u4f53\u7cfb\u8868\u73b0\u51fa\u975e\u5e38\u597d\u7684\u80bf\u7624\u9776\u5411\u6027\u548c\u751f\u7269\u76f8\u5bb9\u6027\uff0c\u53ef\u4f5c\u4e3a\u5168\u65b0\u7684\u75be\u75c5\u8bca\u65ad\u548c\u6cbb\u7597\u5e73\u53f0\u3002<\/p>\n

\u672c\u6587\u7531\u6750\u6599\u4eba\u5b66\u672f\u7ec4gaxy\u4f9b\u7a3f\uff0c\u6750\u6599\u725b\u6574\u7406\u7f16\u8f91\u3002 \u00a0<\/p>\n

\u6750\u6599\u725b\u7f51\u4e13\u6ce8\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u53ca\u884c\u4e1a\u8fdb\u5c55\uff0c\u8fd9\u91cc\u6c47\u96c6\u4e86\u5404\u5927\u9ad8\u6821\u7855\u535a\u751f\u3001\u4e00\u7ebf\u79d1\u7814\u4eba\u5458\u4ee5\u53ca\u884c\u4e1a\u4ece\u4e1a\u8005\uff0c\u5982\u679c\u60a8\u5bf9\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u8fdb\u5c55\uff0c\u89e3\u8bfb\u9ad8\u6c34\u5e73\u6587\u7ae0\u6216\u662f\u8bc4\u8ff0\u884c\u4e1a\u6709\u5174\u8da3\uff0c\u70b9\u6211\u52a0\u5165\u6750\u6599\u4eba\u7f16\u8f91\u90e8<\/a>\u3002<\/p>\n

\u6b22\u8fce\u5927\u5bb6\u5230\u6750\u6599\u4eba\u5ba3\u4f20\u79d1\u6280\u6210\u679c\u5e76\u5bf9\u6587\u732e\u8fdb\u884c\u6df1\u5165\u89e3\u8bfb\uff0c\u6295\u7a3f\u90ae\u7bb1tougao@cailiaoren.com\u3002<\/p>\n

\u6295\u7a3f\u4ee5\u53ca\u5185\u5bb9\u5408\u4f5c\u53ef\u52a0\u7f16\u8f91\u5fae\u4fe1\uff1acailiaokefu\uff0c\u6211\u4eec\u4f1a\u9080\u8bf7\u5404\u4f4d\u8001\u5e08\u52a0\u5165\u4e13\u5bb6\u7fa4\u3002 \u00a0<\/p>\n

\u6750\u6599\u6d4b\u8bd5\u3001\u6570\u636e\u5206\u6790\uff0c\u4e0a\u6d4b\u8bd5\u8c37<\/a>\uff01<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

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