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Encoded Compound Library\uff0c\u7b80\u79f0DEL)\u662f\u5408\u6210\u5206\u5b50\u7684\u6df7\u5408\u7269\uff0c\u6bcf\u4e2a\u5408\u6210\u5206\u5b50\u7531\u5171\u4ef7\u8fde\u63a5\u7684DNA\u6807\u7b7e\u7f16\u7801\u3002\u81ea\u53d1\u73b0\u4ee5\u6765\uff0c\u8fd9\u9879\u6280\u672f\u5df2\u7ecf\u5f97\u5230\u5f88\u597d\u7684\u53d1\u5c55\u3002DEL\u53ef\u4ee5\u5728\u4e00\u79cd\u89e3\u51b3\u65b9\u6848\u4e2d\u5feb\u901f\u4e14\u4fbf\u5b9c\u5730\u540c\u65f6\u6d4b\u8bd5\u6574\u4e2a\u6587\u5e93\uff0c\u4ee5\u4fbf\u4e0e\u611f\u5174\u8da3\u7684\u76ee\u6807\u7ed3\u5408\uff0c\u5e76\u4e14\u53ea\u9700\u8981\u5c11\u91cf\u7684\u751f\u7269\u6750\u6599\uff08\u6bcf\u4e2a\u9009\u62e9\u7ea65-50\u03bcg\u7684\u5178\u578b\u76ee\u6807\u86cb\u767d\uff09\u3002DEL\u6280\u672f\u662f\u7ec4\u5408\u5316\u5b66\u548c\u5206\u5b50\u751f\u7269\u5b66\u7684\u5b8c\u7f8e\u7ed3\u6676\uff0c\u5e76\u5728\u9ad8\u901a\u91cf\u6d4b\u5e8f\u6280\u672f\u7684\u8fc5\u901f\u53d1\u5c55\u4e0b\u5f97\u5230\u4e86\u5de8\u5927\u7684\u63a8\u52a8\uff0c\u4f7f\u5f97\u5148\u5bfc\u5316\u5408\u7269\u7684\u7b5b\u9009\u53d8\u5f97\u524d\u6240\u672a\u6709\u7684\u5feb\u6377\u548c\u9ad8\u6548\u3002<\/p>\n<p>\u5c3d\u7ba1\u6709\u8fd9\u4e9b\u4f18\u52bf\uff0c\u4f46\u7edd\u5927\u591a\u6570DEL\u4ecd\u7136\u5c40\u9650\u4e8e\u5236\u836f\u516c\u53f8\uff0c\u56f4\u7ed5\u5176\u5728\u5de5\u4e1a\u4e2d\u7684\u5f00\u53d1\u548c\u4f7f\u7528\u7684\u8bb8\u591a\u7814\u7a76\u8fdb\u5c55\u4ecd\u672a\u516c\u5f00\u3002\u867d\u7136\u5df2\u7ecf\u62a5\u9053\u4e86\u4e00\u4e9b\u5408\u6210DEL\u7684\u521d\u59cb\u7b56\u7565\u3002\u5728\u4e00\u4e9b\u60c5\u51b5\u4e0b\uff0c\u8fd9\u4e9b\u65b9\u6cd5\u4f7f\u5f97\u6784\u5efa\u5316\u5408\u7269\u6570\u91cf\u9ad8\u8fbe\u6570\u767e\u4e07\u751a\u81f3\u6570\u5341\u4ebf\u7684\u6587\u5e93\u6210\u4e3a\u53ef\u80fd\u3002\u4f46\u4f34\u968f\u7740\u7684\u5374\u662f\u968f\u7740\u6587\u5e93\u5927\u5c0f\u7684\u589e\u52a0\uff0c\u53ef\u88ab\u786e\u8ba4\u7ecf\u5386\u9884\u671f\u53cd\u5e94\u9014\u5f84\u7684\u6587\u5e93\u6210\u5206\u5374\u51cf\u5c11\u3002\u91cd\u8981\u7684\u662f\uff0cDEL\u7684\u8d28\u91cf\u53d6\u51b3\u4e8e\u7531\u5176DNA\u6807\u7b7e\u7f16\u7801\u6b63\u786e\u5408\u6210\u7684\u5206\u5b50\u7684\u6bd4\u4f8b\uff0c\u800c\u6587\u5e93\u7684\u8d28\u91cf\u53c8\u76f4\u63a5\u5f71\u54cd\u9009\u62e9\u7ed3\u679c\u7684\u53ef\u9760\u6027\u3002\u53ef\u662f\uff0c\u5728\u5927\u591a\u6570\u60c5\u51b5\u4e0b\uff0c\u6bcf\u4e2a\u5316\u5b66\u5076\u8054\u6b65\u9aa4\u540e\u90fd\u8fdb\u884c\u4ea7\u7269\u7684\u7eaf\u5316\u662f\u4e0d\u53ef\u884c\u7684\uff0c\u8fd9\u5bfc\u81f4\u8fde\u63a5DNA\u6807\u7b7e\u7684\u622a\u77ed\u7684\u526f\u4ea7\u7269\u6c61\u67d3\u751a\u81f3\u63a7\u5236\u4e86\u6587\u5e93\u3002<\/p>\n<p>\u5f53\u4f7f\u7528\u4f4e\u6548\u79ef\u6728\u4f5c\u4e3a\u6587\u5e93\u8bbe\u8ba1\u7684\u4e00\u90e8\u5206\u6765\u6311\u6218\u8bf8\u5982\u5927\u73af\u5316\u6216\u5076\u8054\u53cd\u5e94\u7b49\u5316\u5b66\u8f6c\u5316\u65f6\uff0c\u8fd9\u79cd\u9650\u5236\u53ef\u80fd\u53d8\u5f97\u7279\u522b\u6210\u95ee\u9898\u3002\u56e0\u6b64\uff0c\u4f7f\u7528\u4f20\u7edf\u7684\u65b9\u6cd5\u6765\u751f\u6210\u9ad8\u8d28\u91cf\u7684\u5927\u73af\u5316\u5408\u7269DEL\u53ef\u80fd\u662f\u4e00\u4e2a\u7279\u522b\u7684\u6311\u6218\uff0c\u9664\u975e\u652f\u67b6\u7684\u5927\u90e8\u5206\u662f\u9884\u5148\u5f62\u6210\u7684\uff0c\u800c\u7ec4\u5408\u53d8\u5316\u4ec5\u9650\u4e8e\u5f15\u5165\u53d6\u4ee3\u57fa\uff0c\u5b9e\u9645\u4e0a\u8fd9\u9650\u5236\u4e86\u6587\u5e93\u7ed3\u6784\u7684\u591a\u6837\u6027\u3002<\/p>\n<p>\u56e0\u6b64\uff0c\u5f00\u53d1\u6784\u5efa\u9ad8\u5ea6\u591a\u6837\u5316DNA\u7f16\u7801\u5927\u73af\u5316\u5408\u7269\u5e93\u7684\u65b9\u6cd5\u662f\u4e00\u4e2a\u5177\u6709\u6311\u6218\u6027\u7684\u76ee\u6807\uff0c\u53ef\u4ee5\u63d0\u4f9b\u6f5c\u5728\u7684\u5316\u5b66\u7a7a\u95f4\u3002 \u5927\u73af\u5206\u5b50\u7684\u751f\u7269\u533b\u5b66\u7279\u6027\u8fdb\u4e00\u6b65\u7a81\u51fa\u4e86\u8fd9\u4e9b\u6587\u5e93\u7684\u5e94\u7528\u6f5c\u529b\u3002 \u76ee\u524d\uff0c\u5927\u7ea670\u79cd\u5927\u73af\u7c7b\u836f\u7269\u5df2\u88ab\u6279\u51c6\u7528\u4e8e\u4eba\u7c7b\u4f7f\u7528\uff0c\u8d85\u8fc735\u79cd\u5927\u73af\u7c7b\u836f\u7269\u5904\u4e8e\u4e34\u5e8a\u5f00\u53d1\u7684\u4e0d\u540c\u9636\u6bb5\u3002\u901a\u5e38\u8ba4\u4e3a\u5927\u73af\u6bd4\u5176\u7ebf\u6027\u5bf9\u5e94\u7269\u5177\u6709\u66f4\u597d\u7684\u4f53\u5185\u7a33\u5b9a\u6027\uff0c\u5e76\u63d0\u4f9b\u4e86\u7075\u6d3b\u6027\u548c\u9884\u7ec4\u7ec7\u4e4b\u95f4\u7684\u5e73\u8861\uff0c\u4f7f\u5f97\u5927\u73af\u5316\u5408\u7269\u53ef\u4ee5\u8de8\u8d8a\u5ef6\u4f38\u7684\u86cb\u767d\u8d28\u7ed3\u5408\u4f4d\u70b9\u8fdb\u884c\u76f8\u4e92\u4f5c\u7528\uff0c\u71b5\u5904\u7406\u4f4e\u4e8e\u76f8\u5e94\u7684\u7ebf\u6027\u5206\u5b50\u3002 \u540e\u4e00\u4e2a\u7279\u5f81\u4f7f\u5b83\u4eec\u5728\u9776\u5411\u8868\u9762\u6216\u86cb\u767d\u8d28 &#8211; \u86cb\u767d\u8d28\u76f8\u4e92\u4f5c\u7528\u65b9\u9762\u5f88\u6709\u524d\uff0c\u800c\u8fd9\u4e9b\u76f8\u4e92\u4f5c\u7528\u53ef\u80fd\u96be\u4ee5\u7528\u5e38\u89c4\u5c0f\u5206\u5b50\u5e93\u8fdb\u884c\u9776\u5411\u3002<\/p>\n<p><strong>\u3010\u6210\u679c\u7b80\u4ecb\u3011<\/strong><\/p>\n<p>DNA\u7f16\u7801\u6587\u5e93\u5df2\u7ecf\u6210\u4e3a\u53d1\u73b0\u751f\u7269\u6d3b\u6027\u5c0f\u5206\u5b50\u7684\u5e7f\u6cdb\u4f7f\u7528\u7684\u8d44\u6e90\uff0c\u5e76\u4e14\u4e0e\u4f20\u7edf\u7684\u5c0f\u5206\u5b50\u6587\u5e93\u76f8\u6bd4\u5177\u6709\u663e\u7740\u7684\u4f18\u52bf\u3002\u8fd1\u65e5\uff0c<strong>\u54c8\u4f5b\u5927\u5b66\u7f8e\u56fd\u6770\u51fa\u5316\u5b66\u5bb6David R. Liu\uff08\u901a\u8baf\u4f5c\u8005\uff09<\/strong>\u5728<strong>Nature <\/strong><strong>Chemistry<\/strong>\u671f\u520a\u4e0a\u53d1\u8868\u4e86\u9898\u4e3a<strong>\u201cSecond-generation DNA-templated macrocycle\u00a0<\/strong><strong>libraries for the discovery of bioactive small molecules\u201d<\/strong>\u7684\u7814\u7a76\u8bba\u6587\u3002\u5728\u6b64\u9879\u7814\u7a76\u4e2d\u91cc\uff0c\u4f5c\u8005\u5f00\u53d1\u5e76\u7cbe\u7b80\u4e86DNA\u7f16\u7801\u548cDNA\u6a21\u677f\u5316\u6587\u5e93\u5408\u6210\u65b9\u6cd5\u7684\u591a\u4e2a\u57fa\u672c\u65b9\u9762\uff0c\u5305\u62ec20\u00d720\u00d720\u00d780\u6b63\u4ea4\u5bc6\u7801\u5b50\u7684\u8ba1\u7b97\u8bc6\u522b\u548c\u5b9e\u9a8c\u9a8c\u8bc1\uff0c\u589e\u5f3a\u7ed3\u6784\u7684\u5316\u5b66\u548c\u8ba1\u7b97\u5de5\u5177\u6587\u5e93\u6210\u5458\u7684\u591a\u6837\u6027\u548c\u836f\u7269\u76f8\u4f3c\u6027\uff0c\u9ad8\u6548\u805a\u5408\u9176\u4ecb\u5bfc\u7684\u6a21\u677f\u6587\u5e93\u7ec4\u88c5\u7b56\u7565\u4ee5\u53ca\u6587\u5e93\u5206\u79bb\u548c\u7eaf\u5316\u65b9\u6cd5\u3002\u4f5c\u8005\u53ca\u5176\u56e2\u961f\u6574\u5408\u4e86\u8fd9\u4e9b\u6539\u8fdb\u7684\u65b9\u6cd5\uff0c\u4ee5\u751f\u6210\u5177\u6709\u6539\u5584\u7684\u836f\u7269\u7269\u7406\u6027\u8d28\u7684\u7b2c\u4e8c\u4ee3DNA\u6a21\u677f\u5e93\uff0c\u5176\u4e2d\u5305\u542b256,000\u4e2a\u5c0f\u5206\u5b50\u5927\u73af\u5316\u5408\u7269\u3002\u8be5\u6587\u5e93\u4f53\u5916\u9009\u62e9\u80f0\u5c9b\u7d20\u964d\u89e3\u9176\u4eb2\u548c\u6027\u4ea7\u751f\u65b0\u9896\u7684\u80f0\u5c9b\u7d20\u964d\u89e3\u9176\u6291\u5236\u5242\uff0c\u5305\u62ec\u5f02\u5e38\u6548\u529b\u548c\u65b0\u578b\u5927\u73af\u5316\u5408\u7269\uff08IC<sub>50<\/sub> = 40nM\uff09\u4e4b\u4e00\u3002\u603b\u7684\u6765\u8bf4\uff0c\u8fd9\u4e9b\u53d1\u5c55\u4f7f\u5f97\u4ee5DNA\u4e3a\u6a21\u677f\u7684\u5c0f\u5206\u5b50\u6587\u5e93\u6210\u4e3a\u7528\u4e8e\u751f\u7269\u6d3b\u6027\u5c0f\u5206\u5b50\u53d1\u73b0\u7684\u66f4\u5f3a\u5927\uff0c\u53ef\u8bbf\u95ee\uff0c\u7b80\u5316\u548c\u5177\u6709\u6210\u672c\u6548\u76ca\u7684\u5de5\u5177<\/p>\n<p><strong>\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/strong><\/p>\n<p><strong>\u56fe1\uff1aDNA\u6a21\u677f\u7684\u5927\u73af\u5316\u5408\u7269\u6587\u5e93\u5408\u6210\u793a\u610f\u56fe<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-133781\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2018\/04\/1-19.jpg\" alt=\"\" width=\"600\" height=\"305\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2018\/04\/1-19.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2018\/04\/1-19-300x153.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>\u5c55\u793a\u4e86\u5148\u524d\u63cf\u8ff0\u7684\u7b2c\u4e00\u4ee3\uff08\u7070\u8272\uff09\u548c\u7b2c\u4e8c\u4ee3\uff08\u9ed1\u8272\uff0c\u5f69\u8272\uff09\u5e93\u5408\u6210\u7684\u5173\u952e\u65b9\u9762\u3002\u5728\u7b2c\u4e00\u6b65\u4e2d\uff0c\u8fde\u63a5\u5728\u6a21\u677f5&#8217;\u7aef\u7684\u652f\u67b6\u7ed3\u6784\u5355\u5143D\u4e0e\u7ed3\u6784\u5355\u5143A\u8fdb\u884c\u5076\u8054\uff0c\u7ed3\u6784\u5355\u5143A\u6700\u521d\u901a\u8fc7\u53ef\u88c2\u89e3\u7684 bis(2-(succinimidooxycarbonyloxy)ethyl) sulfone (BSOCOES) \u8fde\u63a5\u5b50\u8fde\u63a5\u5230\u76f8\u5e94\u7684&#8217;\u53cd\u5bc6\u7801\u5b50&#8217;DNA\u4e0a\u3002\u672a\u53cd\u5e94\u7684\u6a21\u677f\u7528\u4e59\u9178\u9150\u52a0\u5e3d\u5c01\u7aef\u3002\u8fde\u63a5\u5b50\u5728\u9ad8pH\u4e0b\u88ab\u88c2\u89e3\uff0c\u91ca\u653e\u7ed3\u6784\u5355\u5143A\u7684\u6c28\u57fa\uff0c\u968f\u540e\u8fdb\u884c\u6b65\u9aa42\u4e0e\u6784\u4ef6B\u5076\u8054\uff0c\u968f\u540e\u52a0\u5e3d\u548c\u63a5\u5934\u88c2\u89e3\u3002\u5728\u4e0e\u751f\u7269\u7d20\u6216PEG\u6807\u8bb0\u7684Wittig\u8bd5\u5242\u7ed3\u6784\u5355\u5143C\u5076\u8054\u540e\uff0c\u7528\u6297\u751f\u86cb\u767d\u94fe\u83cc\u7d20\u6807\u8bb0\u7684beads\uff08\u7b2c\u4e00\u4ee3\u65b9\u6cd5\uff09\u6216\u51dd\u80f6\u7eaf\u5316\uff08\u7b2c\u4e8c\u4ee3\u65b9\u6cd5\uff09\u4e0b\u62c9\u53ef\u4ee5\u5206\u79bb\u5728\u6240\u6709\u4e09\u4e2a\u6b65\u9aa4\u4e2d\u6210\u529f\u53cd\u5e94\u7684\u90a3\u4e9b\u6a21\u677f\u3002\u9ad8\u7898\u9178\u76d0\u5904\u7406\u88c2\u89e3\u4e19\u9170\u80fa\u90e8\u5206\u7684\u4e8c\u9187\u7247\u6bb5\u4ee5\u63d0\u4f9b\u4e59\u919b\u9170\u57fa\uff0c\u5176\u5728\u6e29\u548c\u7684\u78b1\u6027\u6761\u4ef6\u4e0b\u7ecf\u5386Wittig\u73af\u5316\u3002\u6210\u73af\u4ea7\u7269\u5728\u73af\u5316\uff08\u7b2c\u4e00\u4ee3\u7a0b\u5e8f\uff09\u65f6\u4ecebeads\u4e0a\u6d17\u8131\u4e0b\u6765\u6216\u5728\u805a\u4e19\u70ef\u9170\u80fa\u51dd\u80f6\u4e0a\u7eaf\u5316\uff08\u7b2c\u4e8c\u4ee3\u7a0b\u5e8f\uff09\u3002 EDC\uff0cN-\uff083-\u4e8c\u7532\u6c28\u57fa\u4e19\u57fa\uff09-N&#8217;-\u4e59\u57fa\u78b3\u5316\u4e8c\u4e9a\u80fa\u76d0\u9178\u76d0; sNHS\uff0cN-\u7f9f\u57fa\u78fa\u57fa\u7425\u73c0\u9170\u4e9a\u80fa\u94a0\u76d0\u3002<\/p>\n<p><strong>\u56fe2\uff1a\u7b2c\u4e8c\u4ee3DNA\u6a21\u677f\u6587\u5e93\u7684\u6b63\u4ea4\u5bc6\u7801\u5b50\u96c6\u7684\u9274\u5b9a<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-133782\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2018\/04\/2-18.jpg\" alt=\"\" width=\"600\" height=\"503\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2018\/04\/2-18.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2018\/04\/2-18-300x252.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-133787\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2018\/04\/table1.jpg\" alt=\"\" width=\"600\" height=\"307\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2018\/04\/table1.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2018\/04\/table1-300x154.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>a\uff09\u7b2c\u4e8c\u4ee3\u6a21\u677f\u5e93\u7684\u4e00\u822c\u4f53\u7cfb\u7ed3\u6784\u3002\u8fde\u7eed\u7684Ns\u4e0d\u4ee3\u8868\u968f\u673a\u5e8f\u5217\uff0c\u4f46\u8868\u793a\u5404\u4e2a\u5bc6\u7801\u5b50\u7684\u4f4d\u7f6e\uff1b<\/p>\n<p>b\uff09\u7b2c\u4e8c\u4ee3\u56fe\u4e66\u9986\u7684\u7f16\u7801\u7cfb\u7edf\uff1b<\/p>\n<p>c\uff09\u7528\u4e8e\u8ba1\u7b97\u6b63\u4ea4\u5bc6\u7801\u5b50\u96c6\u7684DNA\u6a21\u677f\u7684\u5efa\u8bae\u6a21\u578b\uff1b 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\uff082\uff09\u548c\uff085\uff09\u8868\u793a\u76f8\u5e94\u7684DTS\u53cd\u5e94\uff08\u03b1\uff0c\u03b2\uff0c\u03b3\uff09\u7684\u8868\u89c2\u8f6c\u6362\u3002<\/p>\n<p><strong>\u56fe3\uff1a\u7b2c\u4e8c\u4ee3DNA\u6a21\u677f\u5316\u5927\u73af\u5316\u5408\u7269\u5e93\u7684\u6784\u5efa\u6a21\u5757<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-133783\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2018\/04\/3-16.jpg\" alt=\"\" width=\"600\" height=\"674\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2018\/04\/3-16.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2018\/04\/3-16-267x300.jpg 267w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>a\uff09\u80fd\u591f\u5c06\u65b0\u7684\u652f\u67b6\u7ed3\u6784\u5e76\u5165DNA\u6a21\u677f\u7684\u5408\u6210\u8def\u7ebf\uff0c\u4f8b\u5982\u652f\u67b64I\u548c4L\uff1b<\/p>\n<p>b\uff09\u811a\u624b\u67b6\u5728\u7b2c\u4e8c\u4ee3\u5927\u578b\u5faa\u73af\u5e93\u4e2d\u9a8c\u8bc1\u548c\u4f7f\u7528\uff0c\u7ea2\u8272\u548c\u7eff\u8272\u7403\u4f53\u5206\u522b\u4ee3\u8868\u6784\u4ef61\u548c3\u7684\u8054\u7cfb\uff1b\u5728\u7b2c\u4e00\u4ee3\u6587\u5e93\u4e2d\u4f7f\u7528\u652f\u67b64A-4H\uff08\u865a\u7ebf\u6846\uff09\uff1b <\/p>\n<p>c\uff09\u7ecf\u8fed\u4ee3\u9009\u62e9\u7684\u6784\u5efa\u6a21\u5757\u6700\u5927\u9650\u5ea6\u5730\u63d0\u9ad8\u4e86\u5e93\u4e0eKihlberg\u7684\u53c2\u6570\u7a7a\u95f4\u7684\u91cd\u53e0\uff0c\u7528\u4e8e\u53e3\u670d\u751f\u7269\u53ef\u7528\u5206\u5b50\u3002 DCM\uff0c\u4e8c\u6c2f\u7532\u70f7; DIPEA\uff0cN\uff0cN-\u4e8c\u5f02\u4e19\u57fa\u4e59\u80fa; DMF\uff0cN\uff0cN-\u4e8c\u7532\u57fa\u7532\u9170\u80fa; HBTU\uff0c2-\uff081H-\u82ef\u5e76\u4e09\u5511-1-\u57fa\uff09-1,1,3,3-\u56db\u7532\u57fa\u8132\u516d\u6c1f\u78f7\u9178\u76d0; TFA\uff0c\u4e09\u6c1f\u4e59\u9178\u3002<\/p>\n<p><strong>\u56fe4\uff1a\u6765\u81ea\u7b2c\u4e8c\u4ee3\u5927\u73af\u6587\u5e93\u548c\u7b2c\u4e00\u4ee3\u6587\u5e93\u7684\u6210\u5458\u7684\u7269\u7406\u53c2\u6570\u5206\u5e03<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-133784\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2018\/04\/4-15.jpg\" alt=\"\" width=\"600\" height=\"275\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2018\/04\/4-15.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2018\/04\/4-15-300x138.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>\u6765\u81ea\u7b2c\u4e8c\u4ee3\u5927\u73af\u6587\u5e93\u7684\u7269\u7406\u53c2\u6570\u663e\u793a\u5728x\u8f74\u7684\u4e0a\u65b9\uff0c\u800c\u6765\u81ea\u7b2c\u4e00\u4ee3\u6587\u5e93\u7684\u7269\u7406\u53c2\u6570\u663e\u793a\u5728x\u8f74\u7684\u4e0b\u65b9\u3002 \u989c\u8272\u8868\u793a\u5728\u53c2\u8003\u6587\u732e\u4e2d\u6240\u63cf\u8ff0\u7684\u7406\u60f3\u7684\u201cbeyond rule of five\u201d\uff08bRo5\uff09\u53c2\u6570\u8303\u56f4\u5185\u90e8\uff08\u84dd\u8272\uff09\u6216\u5916\u90e8\uff08\u7ea2\u8272\uff09\u7684\u503c\u3002<\/p>\n<p><strong>\u56fe5\uff1aDNA\u6a21\u677f\u5e93\u7684\u7ec4\u88c5\u65b9\u6cd5<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-133785\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2018\/04\/5-13.jpg\" alt=\"\" width=\"600\" height=\"432\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2018\/04\/5-13.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2018\/04\/5-13-300x216.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>a\uff09\u7ec4\u88c5\u7b2c\u4e00\u4ee3DNA\u6a21\u677f\u5e93\uff1b<\/p>\n<p>b\uff09\u7528\u4e8e\u7b2c\u4e8c\u4ee3DTS\u5e93\u7684\u5939\u677f\u8fde\u63a5\u7ec4\u88c5\u7684\u4fee\u6539\u7248\u672c\u3002\u5c06\u8fde\u63a5\u7247\u6bb5\u7684\u6570\u91cf\u4ece\u4e24\u4e2a\u589e\u52a0\u5230\u4e09\u4e2a\uff0c\u5927\u5927\u51cf\u5c11\u4e86\u6240\u9700\u7684\u5be1\u6838\u82f7\u9178\u5408\u6210\u7684\u6570\u91cf\uff1b<\/p>\n<p>c\uff09\u901a\u8fc7\u9884\u5907\u7684\u9176\u4fc3\u5f15\u7269\u5ef6\u4f38\u7684\u6a21\u677f\u5e93\u88c5\u914d\u7b56\u7565\u3002<\/p>\n<p><strong>\u56fe6\uff1a\u4f53\u5916\u9009\u62e9256,000\u5143DNA-\u6a21\u677f\u5316\u7684\u5927\u73af\u5316\u5408\u7269\u5e93\u7528\u4e8e\u7ed3\u5408IDe<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-133786\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2018\/04\/6-10.jpg\" alt=\"\" width=\"600\" height=\"532\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2018\/04\/6-10.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2018\/04\/6-10-300x266.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p>a\uff0cb\uff09\u5728\u8ba1\u7b97\u8fc7\u6ee4\u4e5d\u4e2a\u7ed3\u6784\u76f8\u4f3c\u7684\u758f\u6c34\u7ed3\u6784\u5355\u5143\uff081J\uff0c1L\uff0c1M\uff0c1N\uff0c1T\uff0c3E\uff0c3H\uff0c3L\uff0c3R\uff09\u4e4b\u524d\uff08a\uff09\u548c\u4e4b\u540e\uff08b\uff09\u7684IDE\u9009\u62e9\u7ed3\u679c\u3002\u9664\u53bb\u5927\u91cf\u7684\u975e\u7279\u5f02\u6027\u566a\u97f3\u66f4\u7a81\u663e\u51fa\u4e30\u5bcc\u7684DJP *\u7cfb\u5217\u5927\u73af\u5316\u5408\u7269\u3002\u5316\u5408\u7269trans-DJPM\u548ccis-DJIR\u662f\u5728\u65e0DNA\u4e0b\u5316\u5b66\u5408\u6210\u7684\uff0c\u5e76\u4e14\u53d1\u73b0\u5b83\u4eec\u4e0e\u7b2c\u4e00\u4ee3DNA\u6a21\u677f\u5316\u6587\u5e93\u4e2d\u53d1\u73b0\u7684\u53cd\u5f0f-6bK\u548c\u53cd\u5f0f-6bA\u5927\u73af\u7ed3\u6784\u76f8\u5f53\u3002\u786e\u5b9a\u7684\u547d\u4e2d\u8fd8\u5305\u62ec\u4e00\u4e2a\u65e0\u5173\u7684\u3001\u65b0\u7ed3\u6784\u5bb6\u65cf\u7684CODVV\u5927\u73af\u3002 R =\uff08CH<sub>2<\/sub><span style=\"font-size: medium;\"><span style=\"line-height: 24px;\">\uff09<\/span><\/span><sub>2<\/sub>O\uff08CH <sub>2<\/sub>\uff09<sub>2<\/sub>NH<sub>2<\/sub>\uff1b<\/p>\n<p>c\uff09\u901a\u8fc7\u8367\u5149\u5341\u80bd\u5207\u5272\u6d4b\u5b9a\u786e\u5b9a\u7684\u5927\u73af\u547d\u4e2d\u7684\u6d53\u5ea6\u4f9d\u8d56\u6027IDE\u6291\u5236\u66f2\u7ebf\u3002\u8bef\u5dee\u7ebf\u8868\u793a\u5e73\u5747\u503c\u7684\u6807\u51c6\u5dee\u3002\u6bcf\u6b21\u547d\u4e2d\u7684\u987a\u5f0f\u548c\u53cd\u5f0f\u5f02\u6784\u4f53\u7684\u56fe\u5177\u6709\u76f8\u540c\u7684\u989c\u8272\u548c\u6807\u8bb0\u5f62\u72b6\uff0c\u4f46\u53cd\u5f0f\u5f02\u6784\u4f53\u4e3a\u586b\u5145\u7684\u7b26\u53f7\u548c\u987a\u5f0f\u5f02\u6784\u4f53\u4e3a\u7a7a\u5fc3\u7b26\u53f7\u3002 DJPM\u53cd\u5f0f\u5f02\u6784\u4f53\u6bd4\u987a\u5f0f\u5f02\u6784\u4f53\u66f4\u6709\u6548\uff0c\u4f46\u6d4b\u8bd5\u7684\u5176\u4ed6\u547d\u4e2d\u5374\u89c2\u5bdf\u5230\u76f8\u53cd\u7684\u8d8b\u52bf\u3002<\/p>\n<p><strong>\u3010\u5c0f\u7ed3\u3011<\/strong><\/p>\n<p>\u672c\u6587\u5f00\u53d1\u5e76\u5408\u6210\u4e86\u7b2c\u4e8c\u4ee3DNA\u6a21\u677f\u5316\u548cDNA\u7f16\u7801\u7684256,000\u4e2a\u5927\u73af\u5316\u5408\u7269\u6587\u5e93\uff0c\u9002\u7528\u4e8e\u4f53\u5916\u9009\u62e9\u548c\u9ad8\u901a\u91cfDNA\u6d4b\u5e8f\u3002\u5728\u6587\u5e93\u5408\u6210\u8fc7\u7a0b\u4e2d\uff0c\u4f5c\u8005\u5f00\u53d1\u5e76\u5e7f\u6cdb\u4f18\u5316\u4e86DNA\u7f16\u7801\u548cDNA\u6a21\u677f\u5e93\u6280\u672f\u7684\u8bb8\u591a\u57fa\u672c\u65b9\u9762\u3002\u8fd9\u4e9b\u8fdb\u5c55\u5305\u62ec\u4ee5\u4e0b\u5185\u5bb9\uff1a<\/p>\n<p>\uff081\uff09\u63d0\u51fa\u5e76\u5b9e\u9a8c\u9a8c\u8bc1\u4e86\u7528\u4e8e\u9274\u5b9aDTS\u6587\u5e93\u5408\u6210\u7684\u6b63\u4ea4\u5bc6\u7801\u5b50\u7684\u65b0\u6a21\u578b\uff0c\u5176\u5bfc\u81f420\u00d720\u00d720\u00d780\u5bc6\u7801\u5b50\u96c6\u8db3\u4ee5\u652f\u6301\u591a\u8fbe640,000\u4e2a\u6210\u5458\u7684\u6587\u5e93\u3002<\/p>\n<p>\uff082\uff09\u9664\u4e86\u589e\u52a0\u811a\u624b\u67b6\u5bc6\u7801\u5b50\u7684\u6570\u91cf\uff08\u4ece8\u4e2a\u589e\u52a0\u523032\u4e2a\u621680\u4e2a\uff09\u5916\uff0c\u6211\u4eec\u8fd8\u5f00\u53d1\u4e86\u4e00\u79cd\u9ad8\u6548\u7684DNA\u517c\u5bb9\u7684beads\u4e0aBoc\u53bb\u4fdd\u62a4\u65b9\u6cd5\uff0c\u5e76\u9a8c\u8bc1\u4e86\u8bb8\u591a\u65b0\u7684\u6784\u5efa\u6a21\u5757\uff0c\u8fd9\u4e9b\u6784\u5efa\u6a21\u5757\u5171\u540c\u5145\u5206\u6269\u5c55\u4e86\u811a\u624b\u67b6\uff0c\u6784\u5efa\u6a21\u5757\u5316\u5927\u73af\u7684\u591a\u6837\u6027\u3002<\/p>\n<p>\uff083\uff09\u5f00\u53d1\u4e86\u8ba1\u7b97\u673a\u811a\u672c\u4ee5\u5e2e\u52a9\u751f\u6210\u5316\u5408\u7269\u6587\u5e93\u7684\u8ba1\u7b97\u673a\u6570\u636e\u5e93\uff0c\u5e76\u9009\u62e9\u80fd\u591f\u63d0\u9ad8\u6240\u5f97\u5206\u5b50\u9884\u6d4b\u751f\u7269\u5229\u7528\u5ea6\u7684\u6784\u5efa\u6a21\u5757\u3002 <\/p>\n<p>\uff084\uff09\u4e3aDNA\u8fde\u63a5\u7684\u5c0f\u5206\u5b50\u5f00\u53d1\u4e86\u65b0\u7684\u5206\u79bb\u548c\u7eaf\u5316\u65b9\u6cd5\uff0c\u4f7f\u5f97DTS\u6587\u5e93\u66f4\u53ef\u9760\uff0c\u53ef\u6269\u5c55\uff0c\u9ad8\u6536\u76ca\u548c\u6210\u672c\u6548\u76ca\u66f4\u9ad8\uff0c\u5e76\u4e14\u5728\u9009\u62e9\u540e\u80fd\u591f\u56de\u6536\u548c\u56de\u6536\u6587\u5e93\u3002 <\/p>\n<p>\uff085\uff09\u5f00\u53d1\u4e86\u65b0\u7684\u805a\u5408\u9176\u8f85\u52a9\u65b9\u6cd5\u6765\u5408\u6210\u5177\u67095&#8217;\u5316\u5b66\u4fee\u9970\u7684DNA\u6a21\u677f\u6587\u5e93\u3002\u8fd9\u4e9b\u65b9\u6cd5\u63d0\u4f9b\u4e86\u5bf9\u6587\u5e93\u8d28\u91cf\u7684\u66f4\u7cbe\u786e\u63a7\u5236\uff0c\u6d88\u9664\u4e86\u4e0e\u5be1\u6838\u82f7\u9178\u6df7\u5408\u7269\u8fdb\u884c\u53cd\u5e94\u7684\u5fc5\u8981\u6027\uff0c\u5e76\u4e14\u901a\u8fc7\u5728\u94fe\u9709\u6297\u751f\u7269\u7d20\u86cb\u767d\u8fde\u63a5\u7684beads\u4e0a\u4e0d\u53ef\u9760\u7684\u56fa\u5b9a\u548c\u4ece\u6807\u51c6\u6c89\u6dc0\u65b9\u6cd5\u7684\u4e0d\u826f\u6062\u590d\u800c\u4f7f\u6750\u6599\u635f\u5931\u6700\u5c0f\u5316\u3002 <\/p>\n<p>\uff086\uff09\u6700\u540e\uff0c\u901a\u8fc7\u9488\u5bf9IDE\u7684\u4f53\u5916\u9009\u62e9\u9a8c\u8bc1\u4e86\u65b0\u7684\u6587\u5e93\u5408\u6210\u65b9\u6848\uff0c\u5bfc\u81f4\u53d1\u73b0\u5bf9\u5148\u524d\u4f18\u5316\u7684IDE\u6291\u5236\u52426bK\uff08IC<sub>50<\/sub> = 50nM\uff09\u7b49\u6548\u7684\u5927\u73aftrans DJPM\uff0c\u5e76\u4e14\u53d1\u73b0cis-DJIR\uff08IC<sub>50<\/sub> = 40 nM\uff09\uff0c\u8fd9\u662f\u4e00\u79cd\u610f\u60f3\u4e0d\u5230\u7684IDE\u987a\u5f0f\u5927\u73af\u9aa8\u67b6\u914d\u4f53IDE\u6291\u5236\u5242\uff0c\u4ee3\u8868\u4e86\u4e00\u79cd\u65b0\u578b\u7684\u7ed3\u5408IDE\u7684\u5927\u73af\u5316\u5408\u7269\u3002<\/p>\n<p><strong>\u6587\u732e\u94fe\u63a5\uff1a<a href=\"https:\/\/www.nature.com\/articles\/s41557-018-0033-8\" target=\"_blank\" rel=\"noopener\">Second-generation DNA-templated macrocycle\u00a0libraries for the discovery of bioactive small molecules.<\/a>\u00a0(Nat. Chem., 2018, DOI:\u00a010.1038\/s41557-018-0033-8\uff09<\/strong><\/p>\n<p>\u672c\u6587\u7531\u6750\u6599\u4eba\u751f\u7269\u7ec4Jing\u4f9b\u7a3f\u3002<\/p>\n<p>\u6750\u6599\u725b\u7f51\u4e13\u6ce8\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u53ca\u884c\u4e1a\u8fdb\u5c55\uff0c\u8fd9\u91cc\u6c47\u96c6\u4e86\u5404\u5927\u9ad8\u6821\u7855\u535a\u751f\u3001\u4e00\u7ebf\u79d1\u7814\u4eba\u5458\u4ee5\u53ca\u884c\u4e1a\u4ece\u4e1a\u8005\uff0c\u5982\u679c\u60a8\u5bf9\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u8fdb\u5c55\uff0c\u89e3\u8bfb\u9ad8\u6c34\u5e73\u6587\u7ae0\u6216\u662f\u8bc4\u8ff0\u884c\u4e1a\u6709\u5174\u8da3\uff0c<a href=\"\/\/m.iemloyee.com\/25127.html\" target=\"_blank\" rel=\"noopener\">\u70b9\u6211\u52a0\u5165\u6750\u6599\u4eba\u7f16\u8f91\u90e8<\/a>\u3002<\/p>\n<p><strong>\u6750\u6599\u6d4b\u8bd5\u3001\u6570\u636e\u5206\u6790\uff0c\u4e0a<\/strong><strong><a href=\"http:\/\/www.ceshigu.com\/\" target=\"_blank\" rel=\"noopener\">\u6d4b\u8bd5\u8c37<\/a><\/strong><strong>\uff01<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u3010\u5f15\u8a00\u3011 \u65b0\u7684\u751f\u7269\u6d3b\u6027\u5c0f\u5206\u5b50\u914d\u4f53\u7684\u53d1\u73b0\u4ecd\u7136\u662f\u751f\u547d\u79d1\u5b66\u7814\u7a76\u754c\u7684\u6838\u5fc3\u52aa\u529b\u65b9\u5411\u3002\u5e38\u89c1\u7684\u5c0f\u5206\u5b50\u53d1\u73b0\u65b9\u6cd5\u4f9d\u8d56\u4e8e\u7b5b\u9009\u5316\u5b66\u5316\u5408\u7269\u7684\u5927\u578b\u96c6\u5408\uff08\u5e93\uff09\u3002\u5728\u5178\u578b\u7684\u7b5b\u67e5\u6d3b\u52a8\u4e2d\uff0c\u6587\u5e93\u6210\u5458&#8230;<\/p>\n","protected":false},"author":4588,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[215,2],"tags":[3044,3042,3043],"class_list":["post-133779","post","type-post","status-publish","format-standard","hentry","category-jiedu","category-keji","tag-del","tag-dna","tag-3043"],"_links":{"self":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/133779"}],"collection":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/users\/4588"}],"replies":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=133779"}],"version-history":[{"count":7,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/133779\/revisions"}],"predecessor-version":[{"id":134857,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/133779\/revisions\/134857"}],"wp:attachment":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=133779"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=133779"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=133779"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}