{"id":127312,"date":"2018-03-04T14:00:00","date_gmt":"2018-03-04T06:00:00","guid":{"rendered":"\/\/m.iemloyee.com\/?p=127312"},"modified":"2018-02-28T20:13:16","modified_gmt":"2018-02-28T12:13:16","slug":"%e4%b8%8a%e6%b5%b7%e4%ba%a4%e5%a4%a7angew-chem-int-ed-%ef%bc%9a%e4%ba%a4%e8%81%94%e7%9a%84%e6%a0%b8%e9%85%b8%e7%ba%b3%e7%b1%b3%e5%87%9d%e8%83%b6%e7%94%a8%e4%ba%8e%e6%9c%89%e6%95%88%e7%9a%84sirna","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=127312","title":{"rendered":"\u4e0a\u6d77\u4ea4\u5927Angew. Chem. Int. Ed.\uff1a\u4ea4\u8054\u7684\u6838\u9178\u7eb3\u7c73\u51dd\u80f6\u7528\u4e8e\u6709\u6548\u7684siRNA\u9012\u9001\u548c\u6297\u80bf\u7624\u6cbb\u7597"},"content":{"rendered":"

\u3010\u5f15\u8a00\u3011<\/strong><\/p>\n

RNA\u5e72\u6270\uff08RNAi\uff09\u6280\u672f\u662f\u901a\u8fc7\u6539\u53d8siRNA\u5e8f\u5217\u6765\u6291\u5236\u57fa\u56e0\u8868\u8fbe\u5e76\u6cbb\u7597\u96be\u6cbb\u6027\u75be\u75c5\u7684\u5de5\u5177\u3002\u7136\u800c\uff0c\u65e0\u6bd2\u6709\u6548\u7684siRNA\u9012\u9001\u8f7d\u4f53\u7684\u7f3a\u4e4f\u963b\u788d\u4e86RNAi\u7597\u6cd5\u7684\u4e34\u5e8a\u5e94\u7528\u3002\u5728\u8fc7\u53bb\u7684\u51e0\u5341\u5e74\u4e2d\uff0c\u57fa\u56e0\u9012\u9001\u5728\u5f00\u53d1\u75c5\u6bd2\u548c\u975e\u75c5\u6bd2\u8f7d\u4f53\u65b9\u9762\u53d6\u5f97\u4e86\u5de8\u5927\u8fdb\u5c55\uff0c\u4f46\u4ecd\u7136\u5b58\u5728\u91cd\u5927\u6311\u6218\u3002\u75c5\u6bd2\u8f7d\u4f53\u53ef\u4ee5\u6709\u6548\u5730\u8f6c\u67d3\u5e26\u6709\u9776\u57fa\u56e0\u7684\u7ec6\u80de\uff0c\u4f46\u5176\u514d\u75ab\u539f\u6027\u548c\u81f4\u7a81\u53d8\u6bd2\u6027\u5b58\u5728\u6781\u5927\u7684\u9690\u60a3\u3002\u975e\u75c5\u6bd2\u8f7d\u4f53\uff08\u4f8b\u5982\uff1a\u80f6\u675f\u3001\u8102\u8d28\u4f53\u3001\u548c\u65e0\u673a\u7eb3\u7c73\u7c92\u5b50\uff09\u53ef\u4ee5\u5728\u4f53\u5916\u548c\u4f53\u5185\u4f20\u9012siRNAs\uff0c\u5374\u4e5f\u9762\u4e34\u7740\u6548\u7387\u548c\u5b89\u5168\u6027\u95ee\u9898\u3002\u4f5c\u4e3a\u6700\u5e38\u7528\u7684\u975e\u75c5\u6bd2\u8f7d\u4f53\uff0c\u80f6\u675f\u548c\u8102\u8d28\u4f53\u7531\u9633\u79bb\u5b50\u805a\u5408\u7269\u548c\u8102\u8d28\u7ec4\u6210\uff0c\u53ef\u4ee5\u901a\u8fc7\u9759\u7535\u76f8\u4e92\u4f5c\u7528\u6709\u6548\u5730\u538b\u7f29siRNA\u5e76\u5c06\u5b83\u4eec\u9012\u9001\u5230\u9776\u7ec6\u80de\u4e2d\uff0c\u6240\u83b7\u5f97\u7684\u57fa\u56e0\u6c89\u9ed8\u529f\u6548\u4e0e\u8fd9\u4e9b\u8f7d\u4f53\u7684\u9633\u79bb\u5b50\u6027\u8d28\u5bc6\u5207\u76f8\u5173\u3002\u8f7d\u4f53\u7684\u9633\u79bb\u5b50\u7535\u8377\u8d8a\u9ad8\uff0c\u8d8a\u80fd\u6709\u6548\u5730\u5c06siRNA\u7f29\u5408\u5e76\u968f\u540e\u4f7f\u9776\u57fa\u56e0\u7684\u8868\u8fbe\u6c89\u9ed8\uff0c\u4f46\u662f\u968f\u7740\u9633\u79bb\u5b50\u6750\u6599\u7684\u6bd2\u6027\u589e\u5f3a\u4e5f\u4f1a\u51fa\u73b0\u4e25\u91cd\u7684\u526f\u4f5c\u7528\u3002\u6b64\u5916\uff0c\u9633\u79bb\u5b50\u7eb3\u7c73\u9897\u7c92\u53ef\u4e0e\u8840\u6d46\u4e2d\u5e26\u8d1f\u7535\u8377\u7684\u86cb\u767d\u8d28\u4ea7\u751f\u5f3a\u70c8\u7684\u76f8\u4e92\u4f5c\u7528\uff0c\u5bfc\u81f4\u7eb3\u7c73\u8f7d\u4f53\u4e0d\u7a33\u5b9a\u548c\u5faa\u73af\u4e2d\u7684\u5feb\u901f\u6e05\u9664\uff0c\u964d\u4f4e\u4e86RNAi\u7684\u6cbb\u7597\u529f\u6548\u3002\u56e0\u6b64\uff0c\u5bfb\u6c42siRNA\u9012\u9001\u8f7d\u4f53\u8fd8\u6709\u6f2b\u957f\u7684\u9053\u8def\u3002<\/p>\n

\u3010\u6210\u679c\u7b80\u4ecb\u3011<\/strong><\/p>\n

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\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/strong><\/p>\n

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\"\"<\/p>\n

A\uff09\u901a\u8fc70.5\uff05\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u8868\u5f81\u7eb3\u7c73\u51dd\u80f6\uff1b<\/p>\n

B\uff09\u7eb3\u7c73\u51dd\u80f6\u7684AFM\u56fe\u50cf\uff1b<\/p>\n

C\uff09\u4e0d\u540c\u5c3a\u5bf8\u7684\u7eb3\u7c73\u51dd\u80f6\u7684DLS\u5206\u6790\uff1b<\/p>\n

D\uff09\u5728\u4e0d\u540c\u57f9\u517b\u65f6\u95f4\u4e0b\uff0cFAM\u6807\u8bb0\u7684siRNA-L\u548c\u7eb3\u7c73\u51dd\u80f6\u7684\u7ec6\u80de\u6444\u53d6\u7684\u6d41\u5f0f\u7ec6\u80de\u672f\u5206\u6790\uff08FAM\u6d53\u5ea6\uff1a3\u03bcm\uff09\uff1b<\/p>\n

E\uff09\u5728\u4e0d\u540c\u65f6\u95f4\u7528FAM\u6807\u8bb0\u7684siRNA-L\u57f9\u517b\u7684HeLa\u7ec6\u80de\u548c\u7eb3\u7c73\u51dd\u80f6\u7684CLSM\u56fe\u50cf\uff0c\u7ec6\u80de\u6838\u7528DAPI\u67d3\u8272\uff08\u6bd4\u4f8b\u5c3a\u4e3a30 \u03bcm\uff1bFAM\u6d53\u5ea6\uff1a3\u03bcm\uff09\u3002<\/p>\n

\u56fe2. \u4f53\u5916\u57fa\u56e0\u6c89\u9ed8<\/strong><\/p>\n

\"\"<\/p>\n

A\uff09\u7528\u4e0d\u540c\u5242\u91cf\u7684nanogel-siEGFP\u57f9\u517b\u7684U2OS\u22c5EGFP\u7ec6\u80de\u7684\u6d41\u5f0f\u7ec6\u80de\u8367\u5149\u5f3a\u5ea6\uff1b<\/p>\n

B\uff09\u7528\u4e0d\u540c\u5242\u91cf\u7684\u7eb3\u7c73\u51dd\u80f6-siEGFP\u8f6c\u67d3\u7684U2OS\u22c5EGFP\u7ec6\u80de\u7684\u8367\u5149\u663e\u5fae\u955c\u56fe\u50cf\uff08\u6bd4\u4f8b\u5c3a\u4e3a100 \u03bcm\uff09\uff1b<\/p>\n

C\uff09\u7528\u542b\u6297EGFP siRNA\uff08\u6d53\u5ea6\u4e3a100nm\uff09\u7684\u4e0d\u540c\u5236\u5242\u5904\u7406\u540e\uff0cU2OS\u22c5EGFP\u7ec6\u80de\u4e2dEGFP\u8868\u8fbe\u7684\u86cb\u767d\u8d28\u5370\u8ff9\u5206\u6790\uff1b<\/p>\n

D\uff09\u7528\u542b\u6297PLK1 siRNA\uff08\u6d53\u5ea6\u4e3a100nm\uff09\u7684\u4e0d\u540c\u5236\u5242\u5904\u7406\u540e\uff0cMDA-MB-231\u7ec6\u80de\u4e2dPLK1\u8868\u8fbe\u7684\u86cb\u767d\u8d28\u5370\u8ff9\u5206\u6790\u3002<\/p>\n

\u56fe3.\u4f53\u5185\u57fa\u56e0\u6c89\u9ed8\u548c\u4f53\u5185\u6297\u80bf\u7624\u6d3b\u6027<\/strong><\/p>\n

\"\"<\/p>\n

A\uff09\u9759\u8109\u6ce8\u5c04\u6297PLK1 siRNA\u7684\u4e0d\u540c\u5236\u5242\u540e\uff0c\u8bb0\u5f55MDA-MB-231\u5f02\u79cd\u79fb\u690d\u8377\u7624\u5c0f\u9f20\u7684\u80bf\u7624\u4f53\u79ef\uff08\u6bcf\u7ec4n = 3\uff09\uff1b<\/p>\n

B\uff09\u5728\u7b2c12\u5929\u6536\u83b7\u7684\u80bf\u7624\u7684\u56fe\u50cf\u30021-5\u5206\u522b\u4e3a\uff1a\u76d0\u6c34\u3001\u88f8\u9732\u7684siPLK1\u3001\u7eb3\u7c73\u51dd\u80f6-SiScram\u3001lipo2000-siPLK1\u3001\u7eb3\u7c73\u51dd\u80f6-SiPLK1\uff08\u6bd4\u4f8b\u5c3a\u4e3a1cm\uff09\uff1b<\/p>\n

C\uff09\u6700\u540e\u4e00\u6b21\u6ce8\u5c04\u540e24\u5c0f\u65f6\u6536\u96c6\u80bf\u7624\u7ec4\u7ec7\uff0c\u80bf\u7624\u7ec4\u7ec7\u4e2dPLK1\u86cb\u767d\u8d28\u8868\u8fbe\u7684\u86cb\u767d\u8d28\u5370\u8ff9\u5206\u6790\uff1b<\/p>\n

D\uff09\u88f8\u4f53siPLK1\u3001Lipo2000-siPLK1\u3001Nanogel-siPLK1\u7684\u836f\u4ee3\u52a8\u529b\u5b66\uff0c\u7528FAM\u6807\u8bb0siRNA\u3002<\/p>\n

E\uff09\u5206\u522b\u7528Cy5.5\u6807\u8bb0\u7684\u88f8\u9732siPLK1\u3001Lipo2000-siPLK1\u3001Nanogel-siPLK1\u5904\u7406\u7684\u5c0f\u9f20\uff0c\u6ce8\u5c04\u540e24\u5c0f\u65f6\u5c0f\u9f20\u4e0d\u540c\u7ec4\u7ec7\u7684\u8367\u5149\u56fe\u50cf\u3002 1-6\u5206\u522b\u4e3a\uff1a\u80bf\u7624\u3001\u5fc3\u810f\u3001\u809d\u810f\u3001\u813e\u810f\u3001\u80ba\u810f\u3001\u80be\u810f\u3002<\/p>\n

\u3010\u5c0f\u7ed3\u3011<\/strong><\/p>\n

\u7814\u7a76\u901a\u8fc7\u5c06DNA\u8fde\u63a5\u5230PCL\u9aa8\u67b6\u4e0a\u5408\u6210\u5b8c\u5168\u6c34\u6eb6\u6027\u7684DNA-g-PCL\u5237\uff0c\u901a\u8fc7\u6838\u9178\u6742\u4ea4\u8fdb\u4e00\u6b65\u7ec4\u88c5\u4ea4\u8054\u7684\u7eb3\u7c73\u51dd\u80f6\u3002\u5b83\u5177\u6709\u53ef\u8c03\u6574\u7684\u5c3a\u5bf8\u3001\u826f\u597d\u7684\u751f\u7406\u7a33\u5b9a\u6027\uff0c\u5305\u88absiRNA\u7684\u7eb3\u7c73\u51dd\u80f6\u8868\u73b0\u51fa\u5bf9RNA\u9176\u964d\u89e3\u7684\u62b5\u6297\u529b\uff0c\u53ef\u589e\u5f3a\u7684\u7ec6\u80de\u6444\u53d6\u4ee5\u53ca\u66f4\u6709\u6548\u5730\u5c06siRNA\u9012\u9001\u81f3\u80bf\u7624\u4f4d\u70b9\uff0c\u5b9e\u73b0\u4f53\u5916\u548c\u4f53\u5185\u6709\u6548\u7684\u57fa\u56e0\u6c89\u9ed8\u3002\u9664\u4e86PLK1\u4e4b\u5916\uff0c\u901a\u8fc7\u7b80\u5355\u5730\u6539\u53d8siRNA\u5e8f\u5217\uff0c\u53ef\u4ee5\u4f7f\u7528\u8fd9\u79cd\u65b0\u7684\u9012\u9001\u7cfb\u7edf\u6765\u8c03\u8282\u5176\u4ed6\u75be\u75c5\u76f8\u5173\u57fa\u56e0\u3002\u6b64\u5916\uff0c\u8fd8\u53ef\u5c06\u529f\u80fd\u6027\u6838\u9178\uff08\u4f8b\u5982\uff0cmiRNA\uff0cmRNA\uff0c\u957f\u975e\u7f16\u7801RNA\uff09\uff0c\u86cb\u767d\u8d28\u548c\u6d3b\u6027\u9776\u5411\u914d\u4f53\u5f15\u5165\u4ea4\u8054\u7eb3\u7c73\u51dd\u80f6\u4e2d\u4ee5\u6269\u5c55\u5176\u80fd\u529b\u6216\u6539\u5584\u751f\u7269\u533b\u5b66\u5e94\u7528\u3002<\/p>\n

\u6587\u732e\u94fe\u63a5\uff1a<\/strong>A Crosslinked Nucleic Acid Nanogel for Effective siRNA Delivery and Antitumor Therapy<\/a>\u00a0\uff08Angew. Chem. Int. Ed.\uff0c2018\uff0cDOI: 10.1002\/anie.201711242\uff09<\/p>\n

\u672c\u6587\u7531\u6750\u6599\u4eba\u7f16\u8f91\u90e8\u751f\u7269\u6750\u6599\u7ec4Meadow\u4f9b\u7a3f\uff0c\u6750\u6599\u725b\u6574\u7406\u7f16\u8f91\u3002<\/p>\n

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