{"id":123208,"date":"2018-01-30T06:30:20","date_gmt":"2018-01-29T22:30:20","guid":{"rendered":"\/\/m.iemloyee.com\/?p=123208"},"modified":"2018-01-29T11:06:19","modified_gmt":"2018-01-29T03:06:19","slug":"angew-chem-int-ed-%ef%bc%9a%e5%a4%9a%e5%8a%9f%e8%83%bdrna%e5%b9%b2%e6%89%b0%e7%ba%b3%e7%b1%b3%e8%8d%af%e7%89%a9%e7%94%a8%e4%ba%8e%e7%99%8c%e7%97%87%e6%b2%bb%e7%96%97","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=123208","title":{"rendered":"Angew. Chem. Int. Ed.\uff1a\u591a\u529f\u80fdRNA\u5e72\u6270\u7eb3\u7c73\u836f\u7269\u7528\u4e8e\u764c\u75c7\u6cbb\u7597"},"content":{"rendered":"

\u3010\u5f15\u8a00\u3011<\/strong><\/p>\n

\u7f3a\u6c27\u662f\u5b9e\u4f53\u7624\u91cd\u8981\u7684\u7279\u5f81\u3002\u5728\u7f3a\u6c27\u73af\u5883\u4e0b\uff0c\u7624\u4f53\u7ec4\u7ec7\u4ee5\u7cd6\u9175\u89e3\u7684\u65b9\u5f0f\u8fdb\u884c\u4ee3\u8c22\uff0c\u4ece\u800c\u4ea7\u751f\u5927\u91cf\u7684\u4e73\u9178\u548c\u7ec6\u80de\u7e41\u6b96\u6240\u9700\u7684\u80fd\u91cf\u3002\u540c\u65f6\uff0c\u5355\u7fa7\u9178\u8f6c\u8fd0\u86cb\u767d\u8868\u8fbe\u4e0a\u8c03\uff08MCTS\uff09\uff0c\u4ee5\u7ef4\u6301\u80de\u5185\u7684pH\u3002\u6d3b\u6027\u6c27\u7c07\uff08ROS\uff09\u7684\u9ad8\u8868\u8fbe\u4e5f\u662f\u5b9e\u4f53\u7624\u7684\u4e00\u4e2a\u91cd\u8981\u7279\u5f81\u3002\u4f8b\u5982\u7624\u4f53\u7ec6\u80de\u5185H2O2\u91cf\u4e00\u822c\u9ad8\u4e8e100\u03bcM\uff0c\u6b63\u5e38\u7ec6\u80deH2O2\u91cf\u7ea6\u572820nM\u3002\u672c\u6587\u5408\u6210\u4e86\u4e00\u79cd\u88c5\u8f7dsiRNA\u975e\u6676\u6c27\u5316\u94c1\uff08AIO\uff09\u7eb3\u7c73\u836f\u7269\uff08NP(siMCT4) or AIO RNAi NPs\uff09\u3002\u8be5\u836f\u7269\u80fd\u6291\u5236MCTs\u7684\u4e0a\u8c03\uff0c\u4f7f\u7ec6\u80de\u9178\u4e2d\u6bd2\uff0c\u540c\u65f6\u53d1\u751f\u7c7b\u82ac\u987f\u53cd\u5e94\uff08Feton-like\uff09\u50ac\u5316H2O2\u4ea7\u751f\u7f9f\u57fa\u81ea\u7531\u57fa\uff0c\u4ece\u800c\u6740\u6b7b\u80bf\u7624\u7ec6\u80de\uff0c\u6291\u5236\u80bf\u7624\u751f\u957f\u3002<\/p>\n

\u3010\u6210\u679c\u7b80\u4ecb\u3011<\/strong><\/p>\n

\u8fd1\u65e5\uff0c\u54c8\u4f5b\u5927\u5b66\u533b\u5b66\u9662\u65bd\u8fdb\u519b\u6559\u6388\u56e2\u961f<\/strong>\u5728\u80bf\u7624\u6cbb\u7597\u65b9\u9762\u53d6\u5f97\u4e86\u4e00\u5b9a\u7684\u7814\u7a76\u8fdb\u5c55\u3002\u7814\u7a76\u4eba\u5458\u5408\u6210\u4e86\u4e00\u79cd\u88c5\u8f7dsiRNA\u975e\u6676\u6c27\u5316\u94c1\u7eb3\u7c73\u836f\u7269\uff0c\u5305\u88f9\u7684siRNA\u80fd\u6291\u5236MCT4\u7684\u8868\u8fbe\u5bfc\u81f4\u7ec6\u80de\u9178\u4e2d\u6bd2\uff0c\u9178\u6027\u6761\u4ef6\u4e0b\u975e\u6676\u6c27\u5316\u94c1\u80fd\u66f4\u597d\u7684\u53d1\u751f\u7c7b\u82ac\u987f\u53cd\u5e94\u4ea7\u751f\u7ec6\u80de\u6bd2\u6027\u3002\u6b64\u9879\u7814\u7a76\u6210\u679c\u4ee5\u201cEngineering Multifunctional RNAi Nanomedicine To Concurrently Target Cancer Hallmarks for Combinatorial Therapy<\/strong>\u201d\u4e3a\u9898\u53d1\u8868\u5728Angewandte Chemie International Edition<\/strong>\u4e0a\u3002<\/p>\n

\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/strong><\/p>\n

\u56fe1\uff1aAIO RNAi NPs\u8868\u5f81\u53ca\u7ec6\u80de\u5b9e\u9a8c\u3002<\/strong><\/p>\n

\"\"<\/p>\n

\uff08a\uff09AIO RNAi NPs\u7684\u900f\u5c04\u7535\u955c\u56fe\uff1b<\/p>\n

\uff08b\uff09AIO RNAi NPs\u7684X\u5c04\u7ebf\u6676\u4f53\u884d\u5c04\uff08XRD\uff09\uff1b<\/p>\n

\uff08c\uff09\u4e0d\u540c\u6d53\u5ea6\u7684H2O2\u6761\u4ef6\u4e0bAIO RNAi\u7eb3\u7c73\u7c92\u5b50\u7684ESR\u8c31\uff1b<\/p>\n

\uff08d\uff09Cy-5\u6807\u8bb0\u7684AIO RNAi NPs \u4e0eHela\u7ec6\u80de\u5171\u5b75\u80b2\u8367\u5149\u5171\u805a\u7126\u6210\u50cf\uff1b<\/p>\n

\uff08e\uff09\u8367\u5149\u5171\u805a\u7126\u76d1\u6d4b\u7ec6\u80de\u5185\u7f9f\u57fa\u81ea\u7531\u57fa\u4ea7\u751f\uff1b<\/p>\n

\uff08f\uff09\u7528\u56db\u7532\u5416\u5576\uff08AO\uff09\u5bf9\u4e0ePBS\u6216AIO NPs \u5171\u5b75\u80b2\u7684PC3\u7ec6\u80de\u67d3\u8272\u3002AIO NPs\u5904\u7406\u7ec6\u80de\u4e2d\u51cf\u5c11\u7684\u7ea2\u70b9\u8868\u660e\u6eb6\u9176\u4f53\/\u6eb6\u9176\u4f53\u7684\u819c\u5b8c\u6574\u6027\u4e27\u5931\uff1b<\/p>\n

\uff08g\uff09AIO RNAi NPs\u7684\u7ec4\u7ec7\u6e17\u900f\u80fd\u529b\u3002<\/p>\n

\u56fe2\uff1asiRNA\u8c03\u8282\u80de\u5185pH\u86cb\u767d\u4fc3\u8fdb\u7c7b\u82ac\u987f\u53cd\u5e94\u3002<\/strong><\/p>\n

\"\"
\n \uff08a\uff09NP(siControl)\u4e0eNP(siMCT4)\u5904\u7406\u540e\u7684PC3\u7ec6\u80de\u7684MCT4\u86cb\u767d\u8d28\u5370\u8ff9\u5206\u6790\uff1b<\/p>\n

\uff08b\uff09NP(siControl)\u4e0eNP(siMCT4)\u5904\u7406\u540e\u7684PC3\u7ec6\u80de\u7684MCT4\u514d\u75ab\u8367\u5149\u5206\u6790\uff1b<\/p>\n

\uff08c\uff09NP(siControl)\u4e0eNP(siMCT4)\u5904\u7406\u540e\u4e73\u9178\u542b\u91cf\u968f\u65f6\u95f4\u7684\u6539\u53d8\uff1b<\/p>\n

\uff08d\uff09NP(siControl)\u4e0eNP(siMCT4)\u5904\u7406\u540eROS\u7684\u53d8\u5316\uff1b<\/p>\n

\uff08e\uff09PBS\u3001NP(siControl)\u3001NP(siMCT4)\u5904\u7406\u540e\u7684\u7ec6\u80de\u589e\u6b96\uff1b<\/p>\n

\uff08f\uff09\u6d41\u5f0f\u7ec6\u80de\u4eea\u5206\u6790PBS\u5904\u7406\u540ePC3\u7ec6\u80de\u51cb\u4ea1\u60c5\u51b5\uff1b<\/p>\n

\uff08g\uff09\u6d41\u5f0f\u7ec6\u80de\u4eea\u5206\u6790NP(siControl)\u5904\u7406\u540ePC3\u7ec6\u80de\u51cb\u4ea1\u60c5\u51b5\uff1b<\/p>\n

\uff08h\uff09\u6d41\u5f0f\u7ec6\u80de\u4eea\u5206\u6790NP(siMCT4)\u5904\u7406\u540ePC3\u7ec6\u80de\u51cb\u4ea1\u60c5\u51b5\uff1b<\/p>\n

\u00a0 \u8f7d\u6709\u5177\u6709\u6c89\u9ed8MCT4\u86cb\u767d\u7684siRNA\u7684\u7eb3\u7c73\u836f\u7269\u4e0e\u7ec6\u80de\u5171\u5b75\u80b2\u540e\u3002\u7ec6\u80de\u5185\u4e73\u9178\u589e\u52a0\uff0c\u4fc3\u8fdb\u7c7b\u82ac\u987f\u53cd\u5e94\u7684\u53d1\u751f\uff0c\u4ea7\u751f\u66f4\u591a\u7684ROS\u3002<\/p>\n

\u56fe3\uff1a\u5c0f\u9f20\u4ee3\u8c22\u5b9e\u9a8c\u3002<\/strong><\/p>\n

\"\"
\n \uff08a\uff09AIO RNAi NPs\u7684\u836f\u4ee3\u52a8\u529b\u5b66\u66f2\u7ebf\uff1b<\/p>\n

\uff08b\uff09AIO RNAi NPs\u7684\u4ee3\u8c22\u751f\u7269\u5206\u5e03\uff1b<\/p>\n

\uff08c\uff09\u524d\u5217\u817a\u764c\u8377\u7624\u5c0f\u9f20\u9759\u8109\u6ce8\u5c04Cy-5\u6807\u8bb0\u7684AIO RNAi NPs 24 h\u540e\u5668\u5b98\u8367\u5149\u6210\u50cf\uff1b<\/p>\n

\uff08d\uff09\u524d\u5217\u817a\u764c\u8377\u7624\u5c0f\u9f20\u6ce8\u5c04Cy-5\u6807\u8bb0\u7684AIO RNAi NPs 4\u5c0f\u65f6\u540e\u7684\u80bf\u7624\u5207\u7247\u7684\u8367\u5149\u56fe\u50cf\u3002<\/p>\n

\u56fe4\uff1a \u6d3b\u4f53\u5b9e\u9a8c\u3002<\/strong><\/p>\n

\"\"
\n \uff08a\uff09\u524d\u5217\u817a\u764c\u8377\u7624\u5c0f\u9f20\u9759\u8109\u6ce8\u5c04NP(siControl)\u4e0eNP(siMCT4)\u540e\u8fdb\u884cMCT4\u86cb\u767d\u8d28\u5370\u8ff9\u5206\u6790\uff1b<\/p>\n

\uff08b\uff09PBS\u3001NP(siControl)\u3001NP(siMCT4)\u6cbb\u7597\u540e\u5f02\u79cd\u79fb\u690dPC3\u7ec6\u80de\u7684\u5c0f\u9f20\u80bf\u7624\u751f\u957f\u60c5\u51b5\uff1b<\/p>\n

\uff08c\uff09PBS\u3001NP(siControl)\u3001NP(siMCT4)\u6cbb\u7597\u540e\u7684\u8377\u7624\u9f20\u53ca\u80bf\u7624\u7684\u4ee3\u8868\u6027\u56fe\u7247\uff1b<\/p>\n

\uff08d\uff09PBS\u3001NP(siControl)\u3001NP(siMCT4)\u6cbb\u7597\u540e\u7684\u8377\u7624\u9f20\u7684\u6838\u78c1\u6210\u50cf\u3002<\/p>\n

\u3010\u5c0f\u7ed3\u3011<\/strong><\/p>\n

\u672c\u6587\u6210\u529f\u5730\u8bbe\u8ba1\u5e76\u6784\u5efa\u4e86\u4e00\u79cd\u591a\u529f\u80fd\u7684\u7684\u590d\u5408\u7eb3\u7c73\u836f\u7269\u88c5\u8f7dsiRNA\u975e\u6676\u6c27\u5316\u94c1\uff08AIO\uff09\u7eb3\u7c73\u836f\u7269\uff08NP(siMCT4) or AIO RNAi NPs\uff09\u3002\u8be5\u836f\u7269\u5177\u6709\u5de7\u5999\u7684\u534f\u4f5c\u6548\u679c\uff0csiRNA\u6c89\u9ed8MCT4\u86cb\u767d\u8868\u8fbe\uff0c\u4ece\u800c\u589e\u52a0\u4e86\u4e73\u9178\u5728\u7ec6\u80de\u5185\u6ede\u7559\u5bfc\u81f4\u7ec6\u80de\u9178\u4e2d\u6bd2\u3002pH\u503c\u7684\u964d\u4f4e\u8fdb\u4e00\u6b65\u4fc3\u8fdb\u975e\u6676\u6c27\u5316\u94c1\u4e0e\u764c\u7ec6\u80de\u8fc7\u91cf\u8868\u8fbe\u7684H2O2\u53d1\u751f\u7c7b\u82ac\u987f\u53cd\u5e94\uff0c\u4ea7\u751f\u5927\u91cf\u7684\u7f9f\u57fa\u81ea\u7531\u57fa\u3002<\/p>\n

\u6587\u732e\u8fde\u63a5\uff1aEngineering Multifunctional RNAi Nanomedicine To Concurrently Target Cancer Hallmarks for Combinatorial Therapy<\/a><\/strong>\uff08Angew. Chem. Int. Ed. 2018, DOI: 10.1002\/anie.201710144\uff09<\/p>\n

\u672c\u6587\u7531\u6750\u6599\u4eba\u7f16\u8f91\u90e8\u5f20\u96ea\u8c6a\u6574\u7406\u7f16\u8bd1\uff0c\u70b9\u6211\u52a0\u5165\u6750\u6599\u4eba\u7f16\u8f91\u90e8<\/a>\u3002<\/p>\n

\u6750\u6599\u6d4b\u8bd5\uff0c\u6570\u636e\u5206\u6790\uff0c\u4e0a\u6d4b\u8bd5\u8c37<\/a>\uff01<\/p>\n","protected":false},"excerpt":{"rendered":"

\u3010\u5f15\u8a00\u3011 \u7f3a\u6c27\u662f\u5b9e\u4f53\u7624\u91cd\u8981\u7684\u7279\u5f81\u3002\u5728\u7f3a\u6c27\u73af\u5883\u4e0b\uff0c\u7624\u4f53\u7ec4\u7ec7\u4ee5\u7cd6\u9175\u89e3\u7684\u65b9\u5f0f\u8fdb\u884c\u4ee3\u8c22\uff0c\u4ece\u800c\u4ea7\u751f\u5927\u91cf\u7684\u4e73\u9178\u548c\u7ec6\u80de\u7e41\u6b96\u6240\u9700\u7684\u80fd\u91cf\u3002\u540c\u65f6\uff0c\u5355\u7fa7\u9178\u8f6c\u8fd0\u86cb\u767d\u8868\u8fbe\u4e0a\u8c03\uff08MCTS\uff09…<\/p>\n","protected":false},"author":6468,"featured_media":123215,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[215,2],"tags":[116,75,33],"class_list":["post-123208","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-jiedu","category-keji","tag-catalyst","tag-biomaterial","tag-nano"],"_links":{"self":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/123208"}],"collection":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/users\/6468"}],"replies":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=123208"}],"version-history":[{"count":3,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/123208\/revisions"}],"predecessor-version":[{"id":123632,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/123208\/revisions\/123632"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/media\/123215"}],"wp:attachment":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=123208"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=123208"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=123208"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}