{"id":118227,"date":"2017-12-28T06:30:57","date_gmt":"2017-12-27T22:30:57","guid":{"rendered":"\/\/m.iemloyee.com\/?p=118227"},"modified":"2017-12-28T09:00:04","modified_gmt":"2017-12-28T01:00:04","slug":"%e7%9b%98%e7%82%b92017-%e7%a5%9e%e5%99%a8-%e5%86%b7%e5%86%bb%e7%94%b5%e9%95%9c%e4%b9%b1%e5%85%a5%e6%9d%90%e6%96%99%e5%9c%88%ef%bc%9f","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=118227","title":{"rendered":"\u76d8\u70b92017 | 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style=\"color: #0000ff;\"><strong>1. \u4ec0\u4e48\u662f\u51b7\u51bb\u7535\u955c\uff1f<\/strong><\/span><\/p>\n<p>\u51b7\u51bb\u7535\u955c\uff0c\u5168\u79f0\u51b7\u51bb\u7535\u5b50\u663e\u5fae\u955c\u6280\u672f\uff08Cryo-electron microscopy, Cryo-EM\uff09\uff08\u6211\u5927\u6750\u6599\u7684\u5c0f\u4f19\u4f34\u4e5f\u5feb\u597d\u597d\u8bb0\u4f4f\u8fd9\u4e2a\u5355\u8bcd\uff0c\u76f8\u4fe1\u4e0d\u5c31\u7684\u5c06\u6765\u5c31\u4f1a\u6210\u4e3a\u68c0\u7d22\u6750\u6599\u5b66\u6587\u732e\u7684\u70ed\u95e8\u5173\u952e\u8bcd\uff09\uff0c\u662f\u6307\u5c06\u751f\u7269\u5927\u5206\u5b50\u5feb\u901f\u51b7\u51bb\u540e\uff0c\u5728\u4f4e\u6e29\u73af\u5883\u4e0b\u5229\u7528\u900f\u5c04\u7535\u5b50\u663e\u5fae\u955c\u5bf9\u6837\u54c1\u8fdb\u884c\u6210\u50cf\uff0c\u518d\u7ecf\u56fe\u50cf\u5904\u7406\u548c\u91cd\u6784\u8ba1\u7b97\u83b7\u5f97\u6837\u54c1\u4e09\u7ef4\u7ed3\u6784\u7684\u65b9\u6cd5<sup>[1]<\/sup>\u3002\u56fe1\u5c31\u662f\u4e2d\u79d1\u9662\u751f\u7269\u7269\u7406\u7814\u7a76\u6240\u7684FEI Titan Krios 300kV\u51b7\u51bb\u7535\u955c\uff0c\u636e\u8bf4\u5355\u53f0\u5e94\u8be5\u5728600\u4e07\u7f8e\u5143\u4ee5\u4e0a\u3002\u7ecf\u8fc730\u591a\u5e74\u7684\u53d1\u5c55\uff0c\u51b7\u51bb\u7535\u955c\u751a\u81f3\u8d85\u8d8a\u4e86X\u5c04\u7ebf\u6676\u4f53\u5b66\u3001\u6838\u78c1\u5171\u632f\uff08NMR\uff09\u652f\u6491\u8d77\u4e86\u9ad8\u5206\u8fa8\u7387\u7ed3\u6784\u751f\u7269\u5b66\u7814\u7a76\u7684\u57fa\u7840\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-118307\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pd1-1.jpg\" alt=\"\" width=\"600\" height=\"436\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pd1-1.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pd1-1-300x218.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p style=\"text-align: center;\"><strong>\u56fe1 FEI Titan Kiros 300kV \u51b7\u51bb\u7535\u955c\u5b9e\u7269\u56fe<\/strong><\/p>\n<p>\u90a3\u4e48\u4e3a\u4ec0\u4e48\u9700\u8981\u51b7\u51bb\u7535\u955c\u6280\u672f\uff1f\u4f17\u6240\u5468\u77e5\uff0cX\u5c04\u7ebf\u6676\u4f53\u5b66\u662f\u89e3\u6790\u7ed3\u6784\u7684\u7ecf\u5178\u65b9\u6cd5\uff0c\u7136\u800c\u5b83\u9700\u8981\u83b7\u5f97\u751f\u7269\u6837\u54c1\u5355\u6676\uff0c\u751f\u7269\u5927\u5206\u5b50\u7684\u6676\u4f53\u751f\u957f\u5374\u5341\u5206\u56f0\u96be\uff1b\u800c\u4e0e\u6b64\u540c\u65f6\uff0c\u6750\u6599\u5b66\u7814\u7a76\u4e2d\u65e9\u5df2\u4f7f\u7528\u7535\u955c\u76f4\u63a5\u89c2\u5bdf\u5230\u4e86\u539f\u5b50\u50cf<sup>[2]<\/sup>\uff08\u4f5c\u4e3a\u4e00\u540d\u6750\u6599\u6c6a\uff0cTEM\u3001SEM\u7684\u91cd\u8981\u6027\u6211\u60f3\u65e0\u9700\u8d58\u8a00\uff09\uff0c\u4e8e\u662f\u751f\u7269\u5b66\u5bb6\u4e5f\u60f3\u7528\u7535\u955c\u7ed9\u751f\u7269\u5927\u5206\u5b50\u62cd\u4e00\u5f20\u9ad8\u6e05\u7167\u7247\uff0c\u89e3\u6790\u5176\u7ed3\u6784\uff0c\u4ee5\u7406\u89e3\u5176\u751f\u5316\u53cd\u5e94\u673a\u5236\uff0c\u7136\u800c\u4e8b\u60c5\u6ca1\u6709\u90a3\u4e48\u7b80\u5355\uff0c\u7535\u5b50\u663e\u5fae\u955c\u5728\u751f\u7269\u9886\u57df\u7684\u5e94\u7528\u53d7\u5230\u4e86\u4e25\u91cd\u9650\u5236\uff1a\uff081\uff09\u751f\u7269\u6837\u54c1\u542b\u6709\u4e30\u5bcc\u7684\u6c34\uff0c\u800c\u900f\u5c04\u7535\u955c\u7684\u5de5\u4f5c\u6761\u4ef6\u662f\u9ad8\u5ea6\u771f\u7a7a\u7684\uff1b\uff082\uff09\u9ad8\u80fd\u7535\u5b50\u675f\u4f1a\u4e25\u91cd\u7834\u574f\u751f\u7269\u6837\u54c1\uff1b\uff083\uff09\u751f\u7269\u6837\u54c1\u4e3b\u8981\u662fC\u3001O\u3001N\u3001H\u7b49\u8f7b\u5143\u7d20\uff0c\u5bf9\u7535\u5b50\u7684\u53cd\u5c04\u548c\u6563\u5c04\u4e0e\u80cc\u666f\u76f8\u4f3c\uff0c\u83b7\u5f97\u56fe\u50cf\u886c\u5ea6\u5f88\u4f4e\uff1b\uff084\uff09\u86cb\u767d\u8d28\u5206\u5b50\u4f1a\u6f02\u79fb\uff0c\u5bfc\u81f4\u56fe\u50cf\u6a21\u7cca\u3002\u7ecf\u8fc7\u4f17\u591a\u79d1\u5b66\u5bb6\u7684\u957f\u671f\u52aa\u529b\uff0c\u4e0d\u65ad\u514b\u670d\u79cd\u79cd\u56f0\u96be\uff0c\u51b7\u51bb\u7535\u955c\u6280\u672f\u7ec8\u4e8e\u53d1\u5c55\u4e86\u8d77\u6765\uff0c\u5b9e\u73b0\u4e86\u6eb6\u6db2\u91cc\u751f\u7269\u5206\u5b50\u9ad8\u5206\u8fa8\u7387\u7684\u7ed3\u6784\u89e3\u6790\uff0c\u4f7f\u5f97\u751f\u7269\u5316\u5b66\u8fdb\u5165\u4e86\u4e00\u4e2a\u65b0\u65f6\u4ee3\uff0c\u5176\u4e2d3\u4f4d\u6709\u5f00\u521b\u6027\u8d21\u732e\u7684\u79d1\u5b66\u5bb6\u56e0\u6b64\u8363\u81ba2017\u5e74\u8bfa\u8d1d\u5c14\u5316\u5b66\u5956\u3002\u4ed6\u4eec\u5206\u522b\u662f\uff1a\u745e\u58eb\u6d1b\u6851\u5927\u5b66<strong>Jacques Dubochet<\/strong>\u6559\u6388\u3001\u7f8e\u56fd\u54e5\u4f26\u6bd4\u4e9a\u5927\u5b66<strong>Joachim Frank<\/strong>\u6559\u6388\u548c\u82f1\u56fd\u5251\u6865\u5927\u5b66<strong>Richard Henderson<\/strong>\u6559\u6388\u3002\u51b7\u51bb\u7535\u955c\u6280\u672f\u7ed9\u51fa\u7684\u751f\u7269\u5927\u5206\u5b50\u9ad8\u6e05\u7167\u76f8\u7684\u65b9\u6848\u662f<sup>[1]<\/sup>\uff1a<strong>\u6837\u54c1\u51b7\u51bb\u2192\u4f4e\u5242\u91cf\u7535\u5b50\u51b7\u51bb\u6210\u50cf\u2192\u4e09\u7ef4\u91cd\u6784<\/strong>\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-118333\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pd2-2.jpg\" alt=\"\" width=\"600\" height=\"476\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pd2-2.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pd2-2-300x238.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p style=\"text-align: center;\"><strong>\u56fe2 \u83b7\u5f972017\u5e74\u8bfa\u8d1d\u5c14\u5316\u5b66\u5956\u76843\u4f4d\u79d1\u5b66\u5bb6<\/strong><\/p>\n<p><span style=\"color: #ff0000;\"><strong>\uff081\uff09\u6837\u54c1\u51b7\u51bb<\/strong><\/span><\/p>\n<p>\u6837\u54c1\u51b7\u51bb\u5176\u5b9e\u662f\u79d1\u5b66\u5bb6\u4eec\u5f88\u65e9\u5c31\u60f3\u5230\u7684\u601d\u8def\uff0c\u4f46\u662f\u51b7\u51bb\u4e4b\u540e\u6837\u54c1\u4e2d\u6c34\u5206\u5b50\u5f62\u6210\u51b0\u6676\uff0c\u4e0d\u4ec5\u4ea7\u751f\u5f3a\u70c8\u7535\u5b50\u884d\u5c04\u63a9\u76d6\u6837\u54c1\u4fe1\u53f7\uff0c\u8fd8\u4f1a\u6539\u53d8\u6837\u54c1\u7ed3\u6784\u3002\u76f4\u52301974\u5e74\uff0cKenneth A. Taylor\u548cRobert M. Glaeser\u5728-120\u2103\u89c2\u5bdf\u542b\u6c34\u751f\u7269\u6837\u54c1\u65f6\u672a\u53d1\u73b0\u51b0\u6676\u5f62\u6210\uff0c\u800c\u4e14\u53d1\u73b0\u51b7\u51bb\u6837\u54c1\u80fd\u591f\u8010\u53d7\u66f4\u5927\u5242\u91cf\u548c\u66f4\u957f\u65f6\u95f4\u7684\u7535\u5b50\u8f90\u7167\uff0c\u624d\u4e3a\u6837\u54c1\u51b7\u51bb\u5e26\u6765\u8f6c\u673a\u3002\u800c\u4e0a\u9762\u63d0\u5230\u7684Jacques Dubochet\u8001\u7237\u5b50\u5219\u66f4\u8fdb\u4e00\u6b65\uff0c\u53d1\u73b0\u4e86\u6c34\u7684\u73bb\u7483\u6001\uff0c\u6210\u529f\u89e3\u51b3\u4e86\u51b7\u51bb\u7535\u955c\u5236\u6837\u95ee\u9898\uff0c\u5982\u56fe3 \uff08a\uff09\u6240\u793a<sup>[1]<\/sup>\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-118334\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pd3.jpg\" alt=\"\" width=\"600\" height=\"467\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pd3.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pd3-300x234.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p style=\"text-align: center;\"><strong>\u56fe3 \u51b7\u51bb\u7535\u955c\u6837\u54c1\u5236\u5907\uff08a\uff09\u548c\u4e09\u7ef4\u91cd\u6784\uff08b\uff09\u793a\u610f\u56fe<\/strong><\/p>\n<p><span style=\"color: #ff0000;\"><strong>\uff082\uff09\u4f4e\u5242\u91cf\u7535\u5b50\u51b7\u51bb\u6210\u50cf<\/strong><\/span><\/p>\n<p>\u6750\u6599\u6c6a\u90fd\u77e5\u9053\u4e00\u822c\u505aTEM\u3001SEM\u7684\u65f6\u5019\uff0c\u6837\u54c1\u5bfc\u7535\u6027\u8d8a\u597d\uff0c\u7535\u5b50\u5242\u91cf\u8d8a\u9ad8\uff0c\u6210\u50cf\u8d28\u91cf\u8d8a\u597d\u3002\u7136\u800c\uff0c\u9ad8\u5242\u91cf\u7535\u5b50\u5bf9\u751f\u7269\u5927\u5206\u5b50\u5374\u662f\u6bc1\u706d\u6027\u7684\uff0c\u56e0\u6b64Richard Henderson\u6559\u6388\u63d0\u51fa\u5728\u4f4e\u6e29\u4e0b\u7528\u5c3d\u91cf\u4f4e\u7684\u7535\u5b50\u5242\u91cf\u6210\u50cf\u3002\u4ed6\u4e0e\u5176\u5408\u4f5c\u8005\u5148\u540e\u57281975\u5e74\u548c1990\u5e74\u91cd\u6784\u51fa\u4e86\u7c97\u7cd9\u7684\uff087\u00c5\uff09\u548c\u9ad8\u5206\u8fa8\u7387\uff083.5\u00c5\uff09\u7684\u7ec6\u83cc\u89c6\u7d2b\u7ea2\u8d28\u86cb\u767d\u7684\u6a21\u578b\uff0c\u5982\u56fe4\u6240\u793a\uff0c\u8bc1\u660e\u4e86\u51b7\u51bb\u7535\u955c\u7528\u4e8e\u751f\u7269\u5927\u5206\u5b50\u9ad8\u5206\u8fa8\u7387\u7ed3\u6784\u89e3\u6790\u7684\u53ef\u884c\u6027\u3002\u7136\u800c\uff0c\u8fd9\u4e2a\u5386\u65f615\u5e74\u7684\u8fdb\u6b65\u4e0e\u65e9\u57281984\u5e74\u5c31\u83b7\u5f97\u819c\u86cb\u767d3.0 \u00c5\u5206\u8fa8\u7387\u539f\u5b50\u6a21\u578b\u7684<strong>Hartmut Michel<\/strong>\u7b49\u4eba\uff081988\u5e74\u8bfa\u8d1d\u5c14\u5316\u5b66\u5956\u83b7\u5f97\u8005\uff09\u76f8\u6bd4\u4f3c\u4e4e\u4ecd\u663e\u900a\u8272\u3002\u5c3d\u7ba1\u60c5\u51b5\u4e0d\u5bb9\u4e50\u89c2\uff0c\u4f46\u662fHenderson\u6559\u6388\u4ecd\u4e0d\u65ad\u4ece\u7406\u8bba\u4e0a\u6307\u5bfc\u51b7\u51bb\u7535\u955c\u6280\u672f\u7684\u53d1\u5c55\u5e76\u9884\u8a00\uff1a\u968f\u7740\u7535\u955c\u6280\u672f\u548c\u5236\u6837\u6c34\u5e73\u7684\u53d1\u5c55\uff0c\u51b7\u51bb\u7535\u955c\u5fc5\u5c06\u6210\u4e3a\u7591\u96be\u6837\u54c1\u548c\u975e\u7ed3\u6676\u751f\u7269\u5927\u5206\u5b50\u7ed3\u6784\u89e3\u6790\u7684\u6709\u529b\u5de5\u5177\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-118335\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pd4.jpg\" alt=\"\" width=\"600\" height=\"347\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pd4.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pd4-300x174.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p style=\"text-align: center;\"><strong>\u56fe4\u00a0\u7ec6\u83cc\u89c6\u7d2b\u7ea2\u8d28\u86cb\u767d\u76843D\u7ed3\u6784\u6a21\u578b<\/strong><\/p>\n<p><span style=\"color: #ff0000;\"><strong>\uff083\uff09\u4e09\u7ef4\u91cd\u6784<\/strong><\/span><\/p>\n<p>\u505a\u8fc7TEM\u7684\u5c0f\u4f19\u4f34\u90fd\u77e5\u9053\uff0c\u900f\u5c04\u7535\u955c\u5f97\u5230\u7684\u662f\u4e8c\u7ef4\u6295\u5f71\u56fe\u50cf\uff0c\u8981\u5f97\u5230\u4e09\u7ef4\u7684\u7ed3\u6784\uff0c\u5c31\u8981\u901a\u8fc7\u4e00\u7cfb\u5217\u5efa\u6a21\u3001\u53d8\u6362\uff0c\u8fd9\u4e2a\u8fc7\u7a0b\u5c31\u662f\u4e09\u7ef4\u91cd\u6784\u3002\u4e0a\u9762\u63d0\u5230\u7684\u7b2c3\u4f4d\u8bfa\u5956\u5f97\u4e3bJoachim Frank\u5c31\u662f\u548c\u4ed6\u7684\u5408\u4f5c\u8005\u5efa\u7acb\u4e86\u975e\u5bf9\u79f0\u9897\u7c92\u4ece\u4e8c\u7ef4\u6295\u5f71\u5230\u4e09\u7ef4\u7ed3\u6784\u7684\u65b9\u6cd5\uff08\u968f\u673a\u5706\u9525\u503e\u659c\u6cd5\uff09\uff0c\u5960\u5b9a\u4e86\u51b7\u51bb\u7535\u955c\u5355\u9897\u7c92\u4e09\u7ef4\u91cd\u6784\u7684\u57fa\u672c\u539f\u7406\uff0c\u5982\u56fe3\uff08b\uff09\u6240\u793a<sup>[3, 4]<\/sup>\u3002\u968f\u540e\uff0c\u5f00\u53d1\u4e86SPIDER\u7a0b\u5e8f\u7528\u4e8e\u51b7\u51bb\u7535\u955c\u7ed3\u6784\u5206\u6790\uff0c\u5f97\u5230\u4e86\u5e7f\u6cdb\u5e94\u7528\u3002\u76ee\u524d\uff0c\u51b7\u51bb\u7535\u955c\u9886\u57df\u5e7f\u6cdb\u5e94\u7528\u7684\u4e09\u7ef4\u91cd\u6784\u8f6f\u4ef6\u662f\u4e0a\u9762\u5251\u6865\u5927\u5b66Richard Henderson\u8001\u7237\u5b50\u5b9e\u9a8c\u5ba4\u7684<strong>Sjors Scheres<\/strong>\u535a\u58eb\uff08\u636e\u8bf4\u5f53\u65f6Sjors Scheres\u535a\u58eb\u6ca1\u6709\u4e00\u7bc7NSC\u8bba\u6587\uff0c\u4f46Richard Henderson\u6559\u6388\u4ecd\u72ec\u5177\u6167\u773c\u5c06\u5176\u5f15\u8fdb\u5230\u5251\u6865MRC\u5206\u5b50\u751f\u7269\u5b66\u5b9e\u9a8c\u5ba4\uff09\u5f00\u53d1\u7684RELLION\u3002<\/p>\n<p>\u7136\u800c\uff0c\u5373\u4fbf\u6253\u901a\u4e86\u4efb\u7763\u4e8c\u8109\uff08\u4e0a\u8ff03\u4e2a\u5173\u952e\u6d41\u7a0b\uff09\uff0c\u51b7\u51bb\u7535\u955c\u5e76\u6ca1\u6709\u7acb\u5373\u83b7\u5f97\u4eca\u5929\u8fd9\u6837\u7684\u7206\u7ea2\u3002\u8fd9\u4e3b\u8981\u662f\u56e0\u4e3a\uff081\uff09\u51b7\u51bb\u7535\u955c\u7684\u4fe1\u566a\u6bd4\u4f4e\uff0c\uff082\uff09\u56fe\u50cf\u6444\u53d6\u65f6\u6f02\u79fb\uff0c\u4f7f\u5f97\u53ef\u4ee5\u83b7\u53d6\u7684\u4e8c\u7ef4\u6295\u5f71\u4ecd\u662f\u6a21\u7cca\u72b6\u6001\uff0c\u56e0\u6b64\u4ec5\u80fd\u5e94\u7528\u4e8e\u6709\u9650\u7684\u751f\u7269\u5927\u5206\u5b50\u5355\u9897\u7c92\u7684\u7ed3\u6784\u89e3\u6790\uff0c\u4e25\u91cd\u9650\u5236\u4e86\u5176\u5e94\u7528\u3002\u76f4\u52302013\u5e74\uff0c\u52a0\u5dde\u5927\u5b66\u65e7\u91d1\u5c71\u5206\u6821\uff08UCSF\uff09\u7684<strong>\u7a0b\u4ea6\u51e1<\/strong>\u6559\u6388\u7b49\u5c06\u76f4\u63a5\u7535\u5b50\u63a2\u6d4b\u5668\uff08DDD\uff09\u7528\u4e8e\u8bb0\u5f55\u51b7\u51bb\u7535\u955c\u7684\u5355\u9897\u7c92\u56fe\u50cf\uff0c\u5927\u5927\u63d0\u9ad8\u4e86\u4fe1\u566a\u6bd4\u4e0e\u5206\u8fa8\u7387\uff0c\u5b9e\u73b0\u4e86\u8fd1\u539f\u5b50\u5206\u8fa8\u7387\uff083.3 \u00c5\uff09\u7684\u819c\u86cb\u767d\u7ed3\u6784\u7684\u89e3\u6790\uff0c\u624d\u5f15\u8d77\u4e86\u4e1a\u754c\u7684\u8f70\u52a8\uff0c\u5982\u56fe5\u6240\u793a\u3002\u968f\u540e\uff0c\u51b7\u51bb\u7535\u955c\u6280\u672f\u5728\u751f\u7269\u5927\u5206\u5b503D\u7ed3\u6784\u89e3\u6790\u4e2d\u65e0\u5f80\u4e0d\u5229\uff0c\u582a\u79f0\u5c60\u9f99\u5b9d\u5200\u3002\u76ee\u524d\uff0c\u7f8e\u56fdNIH\u7684Subramaniam\u5b9e\u9a8c\u5ba4\u6210\u529f\u89e3\u6790\u4e86\u8c37\u6c28\u9178\u8131\u6c22\u9176\u7684\u7ed3\u6784\uff0c\u5206\u8fa8\u7387\u8fbe\u5230\u4e861.8 \u00c5\uff0c\u521b\u9020\u4e86\u6700\u9ad8\u5206\u8fa8\u7387\u7684\u4e16\u754c\u7eaa\u5f55\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-118336\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pd5.jpg\" alt=\"\" width=\"600\" height=\"387\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pd5.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pd5-300x194.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p style=\"text-align: center;\"><strong>\u56fe5 \u76f4\u63a5\u7535\u5b50\u63a2\u6d4b\u5668\u5e94\u7528\u524d\u540e\u7684\u5206\u8fa8\u7387\u5bf9\u6bd4<\/strong><\/p>\n<p>\u53ef\u89c1\uff0c\u6b63\u662f3\u4f4d\u8bfa\u5956\u79d1\u5b66\u5bb6\u5728\u5404\u81ea\u9886\u57df\u5185\u5b8c\u6210\u7a81\u7834\u6027\u7684\u5de5\u4f5c\uff1aJacques Dubochet\u7a81\u7834\u4e86\u51b7\u51bb\u6280\u672f\u7684\u74f6\u9888\uff0cJoachim Frank\u5728\u4e09\u7ef4\u91cd\u6784\u7b97\u6cd5\u4e0a\u505a\u51fa\u4e86\u539f\u521b\u6027\u8d21\u732e\uff0cRichard Henderson\u9996\u6b21\u4f7f\u7528\u4f4e\u7535\u5b50\u5242\u91cf\u6210\u50cf\u5b8c\u6210\u4e86\u751f\u7269\u5927\u5206\u5b503D\u7ed3\u6784\u7684\u89e3\u6790\u5e76\u4e00\u76f4\u5728\u7406\u8bba\u4e0a\u6307\u5bfc\u51b7\u51bb\u7535\u955c\u6280\u672f\u7684\u53d1\u5c55\uff0c\u6700\u7ec8\u5f62\u6210\u4e860\u52301\u7684\u98de\u8dc3\uff0c\u94f8\u9020\u4e86\u51b7\u51bb\u7535\u955c\u8fd9\u4e00\u628a\u5c60\u9f99\u5b9d\u5200\uff0c\u5f00\u521b\u4e86\u7ed3\u6784\u751f\u7269\u5b66\u7814\u7a76\u7684\u65b0\u5c40\u9762\u3002\u4e0a\u8ff03\u4f4d\u79d1\u5b66\u5bb6\u83b7\u5f97\u8bfa\u8d1d\u5c14\u5956\u7ae0\u53ef\u4ee5\u8bf4\u5f53\u4e4b\u65e0\u6127\uff01<\/p>\n<p><strong><span style=\"color: #0000ff;\">2. \u51b7\u51bb\u7535\u955c\u5728\u7ed3\u6784\u751f\u7269\u5b66\u4e2d\u7684\u6218\u7ee9<\/span><\/strong><\/p>\n<p>\u4eceNSC\u7b49\u9876\u520a\u7684\u53d1\u6587\u60c5\u51b5\u53ca\u6e90\u6e90\u4e0d\u65ad\u7684\u751f\u7269\u5927\u5206\u5b50\u7ed3\u6784\u88ab\u89e3\u6790\u51fa\u6765\uff0c\u51b7\u51bb\u7535\u955c\u5728\u7ed3\u6784\u751f\u7269\u5b66\u9886\u57df\u53d6\u5f97\u7684\u5de8\u5927\u6210\u529f\u65e0\u9700\u8d58\u8ff0\u3002\u5355\u5355\u4ee5\u4e2d\u56fd\u5927\u9646\u4e3a\u4f8b\uff0c\u57fa\u4e8e\u51b7\u51bb\u7535\u955c\u6280\u672f\u5728\u7ed3\u6784\u751f\u7269\u5b66\u9886\u57df\u53d6\u5f97\u7684\u91cd\u5927\u8fdb\u5c55\u5c31\u5341\u5206\u53ef\u89c2\uff0c\u5177\u4f53\u5982\u88681\u6240\u793a<sup>[5]<\/sup>\uff082016\u5e74\uff09\u3002\u800c\u968f\u7740\u51b7\u51bb\u7535\u955c\u6280\u672f\u7684\u5927\u70ed\uff0c\u56fd\u5185\u7684\u8bb8\u591a\u9ad8\u6821\u3001\u79d1\u7814\u9662\u6240\u7eb7\u7eb7\u82b1\u91cd\u91d1\u8d2d\u8fdb\u51b7\u51bb\u7535\u955c\u8bbe\u5907\uff0c\u5df2\u7ecf\u6709\u8d85\u8fc724\u5bb6\u72ec\u7acb\u5b9e\u9a8c\u5ba4\u5728\u91c7\u7528\u51b7\u51bb\u7535\u955c\u8fdb\u884c\u86cb\u767d\u8d28\u7b49\u751f\u7269\u5927\u5206\u5b50\u76843D\u7ed3\u6784\u89e3\u6790\u7814\u7a76\uff0c\u5982\u56fe6\u6240\u793a<sup>[5]<\/sup>\u3002<\/p>\n<p style=\"text-align: center;\"><strong>\u88681 2016\u5e74\u4e2d\u56fd\u5927\u9646\u57fa\u4e8e\u51b7\u51bb\u7535\u955c\u53d6\u5f97\u7684\u6807\u5fd7\u6027\u6210\u679c<\/strong><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-118338\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pdb1.jpg\" alt=\"\" width=\"600\" height=\"636\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pdb1.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pdb1-283x300.jpg 283w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-118337\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pd6.jpg\" alt=\"\" width=\"600\" height=\"495\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pd6.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pd6-300x248.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p style=\"text-align: center;\"><strong>\u56fe6 \u56fd\u5185\u4e3b\u8981\u7684\u51b7\u51bb\u7535\u955c\u5206\u5e03\u56fe<\/strong><\/p>\n<p><span style=\"color: #0000ff;\"><strong>3. \u51b7\u51bb\u7535\u955c\u5728\u6750\u6599\u79d1\u5b66\u4e2d\u5d2d\u9732\u5934\u89d2<\/strong><\/span><\/p>\n<p>\u5c0f\u7f16\u6ca1\u6709\u67e5\u5230\u5728\u5d14\u5c79\u6559\u6388\u4e4b\u524d\u5c06\u51b7\u51bb\u7535\u955c\u6280\u672f\u5e94\u7528\u5230\u6750\u6599\u79d1\u5b66\u9886\u57df\u7684\u62a5\u9053\uff0c\u4f46\u662f\u4e0d\u7ba1\u6709\u6ca1\u6709\uff0c\u4ee5Stanford\u7684<strong>\u5d14\u5c79\u6559\u6388<\/strong>2017\u5e7410\u670827\u65e5\u5728\u7ebf\u53d1\u8868\u5728<strong>Science<\/strong>\u8fd9\u7bc7\u9898\u4e3a\u201c<strong>Atomic structure of sensitive battery materials and interfaces revealed by cryo-electron microscopy<\/strong>\u201d\u7684\u7814\u7a76\u8bba\u6587<sup> [6]<\/sup>\u4f5c\u4e3a\u51b7\u51bb\u7535\u955c\u5728\u6750\u6599\u5b66\u7814\u7a76\u4e2d\u7684\u4e00\u4e2a\u5f00\u7aef\uff0c\u5c0f\u7f16\u8ba4\u4e3a\u662f\u5408\u9002\u7684\uff0c\u6bd5\u7adf\u5b83\u662f\u201c\u4ed6\u5c71\u4e4b\u77f3\uff0c\u53ef\u4ee5\u653b\u7389\u201d\u7684\u4e00\u4e2a\u5178\u8303\uff0c\u53ef\u4ee5\u8bf4\u5f00\u542f\u4e86\u6750\u6599\u79d1\u5b66\u7814\u7a76\u7684\u4e00\u4e2a\u65b0\u4e16\u754c\u3002<\/p>\n<p>\u505a\u9502\u7535\u7684\u5c0f\u4f19\u4f34\u90fd\u77e5\u9053\uff0c\u9502\u679d\u6676\u662f\u9502\u7535\u4e2d\u6700\u5927\u7684\u5b89\u5168\u9690\u60a3\uff0cSamsung\u3001Apple\u4ea7\u54c1\u65f6\u4e0d\u65f6\u51fa\u73b0\u7684\u81ea\u71c3\u4e8b\u6545\u548c\u5b83\u4e0d\u65e0\u5173\u7cfb\u3002\u65f6\u81f3\u4eca\u65e5\uff0c\u679d\u6676\u7684\u4ea7\u751f\u3001\u751f\u957f\u4ee5\u53ca\u523a\u7a7f\u9694\u819c\u9020\u6210\u7535\u6c60\u5185\u90e8\u77ed\u8def\uff0c\u90fd\u662f\u7535\u6c60\u4e13\u5bb6\u4eec\u4e0d\u5f97\u4e0d\u76f4\u9762\u7684\u95ee\u9898\uff0c\u4e5f\u662f\u6750\u6599\u9886\u57df\u201c\u6301\u7eed\u9ad8\u6e29\u201d\u7684\u7814\u7a76\u65b9\u5411\u3002\u7136\u800c\uff0c\u4f17\u6240\u5468\u77e5\uff0c\u9502\u5143\u7d20\u975e\u5e38\u6d3b\u6cfc\uff0c\u5bf9\u73af\u5883\u6781\u5176\u654f\u611f\uff0c\u5982\u4f55\u4ece\u539f\u5b50\u5c42\u9762\u53bb\u7814\u7a76\u9502\u679d\u6676\u7684\u5f62\u6210\u548c\u751f\u957f\uff0c\u6781\u5177\u6311\u6218\u3002\u4f20\u7edf\u7684\u9ad8\u5206\u8fa8TEM\u7535\u5b50\u675f\u80fd\u91cf\u5f88\u9ad8\uff0c\u4f1a\u4e25\u91cd\u635f\u574f\u679d\u6676\u7ed3\u6784\u751a\u81f3\u7194\u6bc1\uff1b\u800c\u4f4e\u5206\u8fa8\u7684TEM\u3001\u76f4\u63a5\u6210\u50cf\u3001\u8868\u9762\u63a2\u9488\u7b49\u6280\u672f\u83b7\u5f97\u7684\u4fe1\u606f\u53c8\u5341\u5206\u6709\u9650\u3002\u5728\u8fd9\u7bc7Science\u8bba\u6587\u4e2d\uff0c\u5d14\u5c79\u6559\u6388\u7b49\u53d7\u201c\u51b7\u51bb\u7535\u955c\u53ef\u4ee5\u83b7\u5f97\u8106\u5f31\u7684\u751f\u7269\u5927\u5206\u5b50\u539f\u5b50\u7ea7\u522b\u7ed3\u6784\u201d\u7684\u542f\u53d1\uff0c\u521b\u9020\u6027\u5730\u5c06\u51b7\u51bb\u7535\u955c\u6280\u672f\u5f15\u5165\u5230\u4e86\u654f\u611f\u6027\u7535\u6c60\u6750\u6599\u548c\u754c\u9762\u7cbe\u7ec6\u7ed3\u6784\u7684\u7814\u7a76\u4e2d\uff0c\u514b\u670d\u4e86\u7535\u6c60\u6750\u6599\u51b7\u51bb\u5236\u6837\u7684\u79cd\u79cd\u96be\u9898\uff0c\u9996\u6b21\u83b7\u5f97\u4e86\u9502\u679d\u6676\u539f\u5b50\u5206\u8fa8\u7387\u7ea7\u522b\u7684\u7ed3\u6784\u56fe\u50cf\u3002\u7ed3\u679c\u663e\u793a\uff0c\u51b7\u51bb\u7535\u955c\u6280\u672f\u5b8c\u6574\u5730\u4fdd\u7559\u4e86\u679d\u6676\u7684\u539f\u59cb\u5f62\u8c8c\u53ca\u76f8\u5173\u7ed3\u6784\u3001\u5316\u5b66\u4fe1\u606f\uff0c\u5728\u6301\u7eed10min\u7684\u7535\u5b50\u675f\u8f70\u51fb\u4e0b\u4ecd\u7136\u4fdd\u6301\u5b8c\u597d\u3002\u9ad8\u5206\u8fa8\u7684Cryo-EM\u7167\u7247\u8868\u660e\u9502\u679d\u6676\u662f\u5448\u957f\u6761\u72b6\u7684\u5b8c\u7f8e\u516d\u9762\u6676\u4f53\uff0c\u5b8c\u5168\u8fe5\u5f02\u4e8e\u4f20\u7edf\u7535\u955c\u89c2\u5bdf\u5230\u7684\u4e0d\u89c4\u5219\u5f62\u72b6\uff1b\u800c\u5176\u751f\u957f\u884c\u4e3a\u663e\u793a\u5176\u6709\u660e\u663e\u7684&lt;111&gt;\u4f18\u5148\u53d6\u5411\uff0c\u751f\u957f\u8fc7\u7a0b\u4e2d\u53ef\u80fd\u53d1\u751f\u201c\u62d0\u5f2f\u201d\uff0c\u4f46\u662f\u5e76\u6ca1\u6709\u5f62\u6210\u6676\u4f53\u7f3a\u9677\uff0c\u4e0d\u5f71\u54cd\u5176\u5b8c\u7f8e\u6676\u4f53\u7ed3\u6784\u3002\u53e6\u5916\uff0c\u7814\u7a76\u7ed3\u679c\u8fd8\u5305\u542b\u56fa\u6001\u7535\u89e3\u8d28\u754c\u9762\uff08SEI\uff09\u7684\u7ec4\u6210\u4e0e\u7ed3\u6784\u3002\u5d14\u5c79\u6559\u6388\u8868\u793a\uff0c\u7814\u7a76\u7ed3\u679c\u5341\u5206\u4ee4\u4eba\u5174\u594b\uff0c\u8bc1\u660e\u4e86Cryo-EM\u53ef\u4ee5\u6709\u6548\u5730\u5bf9\u90a3\u4e9b\u8106\u5f31\u3001\u4e0d\u7a33\u5b9a\u7684\u7535\u6c60\u6750\u6599\u8fdb\u884c\u9ad8\u5206\u8fa8\u7387\u8868\u5f81\uff0c\u4f8b\u5982\u9502\u7845\u3001\u786b\u7b49\uff0c\u5e76\u4e14\u4fdd\u6301\u5b83\u4eec\u5728\u771f\u5b9e\u7535\u6c60\u4e2d\u7684\u539f\u59cb\u72b6\u6001\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-118401\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pd7.jpg\" alt=\"\" width=\"600\" height=\"276\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pd7.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pd7-300x138.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p style=\"text-align: center;\"><strong>\u56fe7 \u901a\u8fc7Cryo-EM\u4fdd\u5b58\u548c\u7a33\u5b9a\u9502\u91d1\u5c5e<\/strong><\/p>\n<p>\u5c0f\u7f16\u5728\u67e5\u9605\u6587\u732e\u8fc7\u7a0b\u4e2d\uff0c\u4e5f\u53d1\u73b0\u4e86\u53e6\u4e00\u7bc7\u91c7\u7528Cryo-EM\u7814\u7a76\u9502\u7535\u6c60\u7684\u8bba\u6587\u201c<strong>New Insights on the Structure of Electrochemically Deposited Lithium Metal and Its Solid Electrolyte Interphases via Cryogenic TEM<\/strong>\u201d\uff0c\u7531\u7f8e\u56fd<strong>\u52a0\u5dde\u5927\u5b66\u5723\u5730\u4e9a\u54e5\u5206\u6821<\/strong>\uff08UCSD\uff09\u7684<strong>\u5b5f\u9896\u6559\u6388<\/strong>\uff08Ying Shirley Meng\uff09\u7b49\u4eba\u53d1\u8868\u5728<strong>Nano Lett.<\/strong>\u4e0a\uff0c\u53d1\u8868\u65f6\u95f4\u4e3a2017\u5e7411\u67081\u65e5\uff0c\u5728\u7ebf\u53d1\u8868\u4ec5\u6bd4\u5d14\u6559\u6388\u7684Science\u5927\u4f5c\u665a4\u5929\u3002\u6587\u7ae0<sup>[9]<\/sup>\u540c\u6837\u662f\u91c7\u53d6\u4e86\u51b7\u51bb\u7535\u955c\u6280\u672f\u7a33\u5b9a\u4e86\u7535\u5316\u5b66\u6c89\u79ef\u7684\u6d3b\u6cfc\u7684\u9502\u91d1\u5c5e\uff0c\u540c\u65f6\u51cf\u5c11\u7535\u5b50\u675f\u5e26\u6765\u7684\u635f\u4f24\uff0c\u7136\u540e\u5bf9\u5176\u7eb3\u7c73\u7ed3\u6784\u3001\u5316\u5b66\u7ec4\u6210\u4ee5\u53ca\u56fa\u6001\u7535\u89e3\u8d28\u754c\u9762\u8fdb\u884c\u4e86\u7814\u7a76\u3002\u53ef\u4ee5\u8bf4\u548c\u5d14\u5c79\u6559\u6388\u5f02\u66f2\u540c\u5de5\uff0c\u8bc1\u660e\u4e86Cryo-EM\u662f\u7814\u7a76\u5bf9\u7535\u5b50\u675f\u3001\u70ed\u654f\u611f\u7684\u7535\u6c60\u6750\u6599\u5f3a\u6709\u529b\u7684\u5de5\u5177\uff0c\u80fd\u591f\u4ece\u6700\u57fa\u7840\u7684\u5c42\u9762\u83b7\u5f97\u76f8\u5173\u4fe1\u606f\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-118402\" src=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pd8.jpg\" alt=\"\" width=\"600\" height=\"181\" srcset=\"\/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pd8.jpg 600w, \/\/m.iemloyee.com\/wp-content\/uploads\/2017\/12\/pd8-300x91.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<p style=\"text-align: center;\"><strong>\u56fe8 Cryo-EM\u7528\u4e8e\u7535\u6c89\u79efLi\u91d1\u5c5e\u7684\u7814\u7a76<\/strong><\/p>\n<p>\u9664\u4e86\u4e0a\u8ff0\u4e24\u7bc7\u5c06Cryo-EM\u7528\u4e8e\u9502\u7535\u6c60\u4e2d\u654f\u611f\u7535\u6c60\u6750\u6599\u53caSEI\u7814\u7a76\u7684\u8bba\u6587\u5916\uff0c\u5c31\u5c0f\u7f16\u6709\u9650\u7684\u8ba4\u77e5\uff0c\u53ef\u80fd\u5728\u6709\u673a\/\u65e0\u673a\u6742\u5316\u9499\u949b\u77ff\u6750\u6599\u3001\u67d0\u4e9b\u9ad8\u5206\u5b50\u6750\u6599\u3001\u6c34\u51dd\u80f6\u3001\u91cf\u5b50\u70b9\u7b49\u7cbe\u7ec6\u7ed3\u6784\u3001\u4e2d\u95f4\u6001\u7684\u8868\u5f81\u4e2d\uff0cCryo-EM\u5c06\u5177\u6709\u7684\u4f18\u52bf\u4e5f\u662f\u4e0d\u8a00\u800c\u55bb\u7684\u3002\u53ef\u4ee5\u9884\u89c1\uff0c\u4e0d\u4e45\u7684\u5c06\u6765\uff0c\u8fd9\u4e9b\u5bf9\u7535\u5b50\u675f\u3001\u70ed\u654f\u611f\u7684\u6d3b\u6cfc\u6750\u6599\u7684\u539f\u5b50\u7ea7\u522b\u7684\u8868\u5f81\u53ef\u80fd\u4f1a\u662fCryo-EM\u5728\u6750\u6599\u9886\u57df\u5e94\u7528\u7684\u6f5c\u529b\u65b9\u5411\u3002<\/p>\n<p><strong><span style=\"color: #0000ff;\">4. \u603b\u7ed3<\/span><\/strong><\/p>\n<p>2017\u5e7410\u67084\u65e5\uff0c\u8bfa\u8d1d\u5c14\u5316\u5b66\u5956\u4e00\u516c\u5e03\u5c31\u5f15\u8d77\u4e86\u670b\u53cb\u5708\u7684\u75af\u72c2\u8c03\u4f83\uff0c\u8ba4\u4e3a\uff1a\u51b7\u51bb\u7535\u955c\u662f\u6388\u4e88\u7269\u7406\u5b66\u5bb6\u7684\u5316\u5b66\u5956\u4ee5\u5956\u52b1\u4ed6\u4eec\u5bf9\u751f\u7269\u9886\u57df\u7684\u6770\u51fa\u8d21\u732e\uff0c\u8ba9\u4f17\u591a\u5316\u5b66\u6c6a\u6df1\u4ee5\u4e3a\u7136\u3002\u73b0\u5728\uff0c\u5d14\u5c79\u6559\u6388\u7684Science\u8bba\u6587\u548c\u5b5f\u9896\u6559\u6388\u7684Nano Lett.\u8bba\u6587\u7ec8\u4e8e\u8ba9\u8fd9\u4e2a\u5316\u5b66\u5956\u591a\u4e86\u4e00\u4e9b\u5316\u5b66\u7684\u610f\u601d\u4e86\u3002\u8fd9\u79cd\u9694\u58c1\u751f\u7269\u5bb6\u7684\u5b69\u5b50\u201c\u8de8\u754c\u201d\u6750\u6599\u5708\u5b8c\u6210\u7684\u4ee4\u4eba\u5174\u594b\u7684\u5de5\u4f5c\u5df2\u7ecf\u9690\u9690\u6709\u64ac\u52a8\u6750\u6599\u5b66\u76f8\u5173\u7814\u7a76\u7684\u82d7\u5934\u4e86\u3002\u5c0f\u7f16\u76f8\u4fe1\uff0c\u8fd9\u9879\u5f15\u9886\u751f\u7269\u5316\u5b66\u7814\u7a76\u8fdb\u5165\u65b0\u65f6\u4ee3\u7684\u6280\u672f\uff0c\u6405\u52a8\u6211\u5927\u6750\u6599\u6c5f\u6e56\u4e5f\u662f\u6307\u65e5\u53ef\u671f\uff01<\/p>\n<p><span style=\"color: #0000ff;\"><strong>\u53c2\u8003\u6587\u732e<\/strong><\/span><\/p>\n<p>[1] \u6768\u6167, \u674e\u614e\u6d9b, \u859b\u51b0. \u51b7\u51bb\u7535\u955c\u6280\u672f: \u4ece\u539f\u5b50\u5c3a\u5ea6\u770b\u751f\u547d[J]. \u9996\u90fd\u533b\u79d1\u5927\u5b66\u5b66\u62a5, 2017, 38(5): 770-776.<\/p>\n<p>[2] \u67f3\u6b63, \u5f20\u666f\u5f3a. \u7ed3\u6784\u751f\u7269\u5b66\u7814\u7a76\u65b9\u6cd5\u7684\u91cd\u5927\u7a81\u7834\u2014\u2014\u7535\u5b50\u76f4\u63a5\u63a2\u6d4b\u76f8\u673a\u5728\u51b7\u51bb\u7535\u955c\u4e2d\u7684\u5e94\u7528[J]. \u751f\u7269\u7269\u7406\u5b66\u62a5, 2014, 30(6): 405-415.<\/p>\n<p>[3] \u5c39\u957f\u57ce. \u541b\u6b32\u5584\u5176\u4e8b\uff0c\u5fc5\u5148\u5229\u5176\u5668\uff01\u2014\u20142017\u5e74\u8bfa\u8d1d\u5c14\u5316\u5b66\u5956\u8bc4\u4ecb[J]. \u4e2d\u56fd\u751f\u7269\u5316\u5b66\u4e0e\u5206\u5b50\u751f\u7269\u5b66\u62a5, 2017, 33(10): 979-984.<\/p>\n<p>[4] Milne J L S, Borgnia M J, Bartesaghi A, et al. Cryo-electron microscopy-a primer for the non-microscopist[J]. FEBS Journal, 2013, 280: 28-45.<\/p>\n<p>[5] Wang H, Lei J, Shi Y. Biological cryo-electron microscopy in China[J]. Protein Science, 2017, 26: 16-31.<\/p>\n<p>[6] Li Y, Li Y, Pei A, et al. Atomic structure of sensitive battery materials and interfaces revealed by cryo-electron microscopy[J]. Science, 2017, 358: 506-510.<\/p>\n<p>[7] \u6750\u6599\u725b: \u5d14\u5c79\u6700\u65b0science:\u73a9\u8f6c\u51b7\u51bb\u7535\u955c\u2014\u2014\u63ed\u5bc6\u7535\u6c60\u6750\u6599\u548c\u754c\u9762\u539f\u5b50\u7ed3\u6784\uff0c\/\/m.iemloyee.com\/108826.html.<\/p>\n<p>[8] X-MOL: \u5316\u5b66\u8bfa\u5956\u51b7\u51bb\u7535\u955c\u518d\u653e\u5f02\u5f69\uff0c\u5d14\u5c79\u56e2\u961f\u5e26\u6765\u91cd\u78c5Science\uff0chttp:\/\/www.x-mol.com:8081\/news\/9696.<\/p>\n<p>[9] Wang X, Zhang M, Alvarado J, et al. New Insights on the Structure of Electrochemically Deposited Lithium Metal and Its Solid Electrolyte Interphases via Cryogenic TEM[J]. Nano Letter, 2017, 17: 7606-7612.<\/p>\n<p>\u672c\u6587\u7531<strong>\u6750\u6599\u4eba\u7f16\u8f91\u90e8\u7eb3\u7c73\u5b66\u672f\u7ec4Roay<\/strong>\u4f9b\u7a3f\u3002<\/p>\n<p>\u6750\u6599\u4eba\u7f51\u4e13\u6ce8\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u53ca\u884c\u4e1a\u8fdb\u5c55\uff0c\u8fd9\u91cc\u6c47\u96c6\u4e86\u5404\u5927\u9ad8\u6821\u7855\u535a\u751f\u3001\u4e00\u7ebf\u79d1\u7814\u4eba\u5458\u4ee5\u53ca\u884c\u4e1a\u4ece\u4e1a\u8005\uff0c\u5982\u679c\u60a8\u5bf9\u4e8e\u8ddf\u8e2a\u6750\u6599\u9886\u57df\u79d1\u6280\u8fdb\u5c55\uff0c\u89e3\u8bfb\u9ad8\u6c34\u5e73\u6587\u7ae0\u6216\u662f\u8bc4\u8ff0\u884c\u4e1a\u6709\u5174\u8da3\uff0c<a href=\"\/\/m.iemloyee.com\/25127.html\" target=\"_blank\" rel=\"noopener noreferrer\">\u70b9\u6211\u52a0\u5165\u7f16\u8f91\u90e8<\/a>\u3002<\/p>\n<p>\u6b22\u8fce\u5927\u5bb6\u5230\u6750\u6599\u4eba\u5ba3\u4f20\u79d1\u6280\u6210\u679c\u5e76\u5bf9\u6587\u732e\u8fdb\u884c\u6df1\u5165\u89e3\u8bfb\uff0c\u6295\u7a3f\u90ae\u7bb1tougao@cailiaoren.com\u3002<\/p>\n<p><strong>\u6295\u7a3f\u4ee5\u53ca\u5185\u5bb9\u5408\u4f5c\u53ef\u52a0\u7f16\u8f91\u5fae\u4fe1\uff1aRDD-2011-CHERISH\uff0c\u4efb\u4e39\u4e39\uff0c\u6211\u4eec\u4f1a\u9080\u8bf7\u5404\u4f4d\u8001\u5e08\u52a0\u5165\u4e13\u5bb6\u7fa4\u3002<\/strong><\/p>\n<p><strong>\u6750\u6599\u6d4b\u8bd5\u3001\u6570\u636e\u5206\u6790\uff0c\u4e0a<a href=\"http:\/\/www.ceshigu.com\/\" target=\"_blank\" rel=\"noopener noreferrer\">\u6d4b\u8bd5\u8c37<\/a>\uff01<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u8bf4\u8d77\u51b7\u51bb\u7535\u955c\uff0c\u5c0f\u7f16\u60f3\u4e0d\u7ba1\u662f\u7814\u7a76\u751f\u8fd8\u662f\u6559\u6388\u5927\u5496\uff0c\u53ef\u80fd\u548c\u79d1\u7814\u6709\u90a3\u4e48\u4e00\u4e01\u70b9\u8054\u7cfb\u7684\u4eba\u5bf9\u8fd9\u4e2a\u540d\u5b57\u90fd\u4e0d\u4f1a\u964c\u751f\uff0c\u56e0\u4e3a\u5b83\u5b9e\u5728\u592a\u51fa\u540d\u4e86\uff01\u57fa\u4e8e\u51b7\u51bb\u7535\u955c\u4ea7\u51fa\u7684\u79d1\u7814\u6210\u679c\u5f88\u591a\u90fd\u53d1\u8868\u5728Na&#8230;<\/p>\n","protected":false},"author":3727,"featured_media":118309,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[155,2482,981,129],"class_list":["post-118227","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-ganhuo","tag-science","tag-2482","tag-test","tag-battery"],"_links":{"self":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/118227"}],"collection":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/users\/3727"}],"replies":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=118227"}],"version-history":[{"count":35,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/118227\/revisions"}],"predecessor-version":[{"id":119275,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/118227\/revisions\/119275"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/media\/118309"}],"wp:attachment":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=118227"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=118227"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=118227"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}