{"id":100938,"date":"2017-09-12T14:00:39","date_gmt":"2017-09-12T06:00:39","guid":{"rendered":"\/\/m.iemloyee.com\/?p=100938"},"modified":"2017-09-10T14:20:18","modified_gmt":"2017-09-10T06:20:18","slug":"%e5%93%88%e5%b7%a5%e5%a4%a7%e6%9d%8e%e4%bf%9d%e5%bc%ba%e5%89%af%e6%95%99%e6%8e%88%ef%bc%9a%e5%9f%ba%e4%ba%8e%e7%ba%b3%e7%b1%b3%e7%a2%b3%e7%82%b9%e5%ae%9e%e6%97%b6%e6%97%a0%e5%88%9b%e5%8f%af%e8%a7%86","status":"publish","type":"post","link":"\/\/m.iemloyee.com\/?p=100938","title":{"rendered":"\u54c8\u5de5\u5927\u674e\u4fdd\u5f3a\u526f\u6559\u6388\uff1a\u57fa\u4e8e\u7eb3\u7c73\u78b3\u70b9\u5b9e\u65f6\u65e0\u521b\u53ef\u89c6\u5316\u76d1\u6d4b\u751f\u7269\u6750\u6599\u4f53\u5185\u964d\u89e3\u7814\u7a76\u53d6\u5f97\u7a81\u7834"},"content":{"rendered":"

\u3010\u6210\u679c\u7b80\u4ecb\u3011<\/strong><\/p>\n

\u54c8\u5de5\u5927\u6750\u6599\u5b66\u9662\u5468\u7389\u9662\u58eb\u56e2\u961f\u9aa8\u5e72\u6210\u5458\u674e\u4fdd\u5f3a\u526f\u6559\u6388\u5728\u57fa\u4e8e\u7eb3\u7c73\u78b3\u70b9\uff08CNDs\uff09\u5b9e\u65f6\u65e0\u521b\u53ef\u89c6\u5316\u76d1\u6d4b\u751f\u7269\u6750\u6599\u4f53\u5185\u964d\u89e3\u65b9\u9762\u53d6\u5f97\u91cd\u8981\u8fdb\u5c55\uff0c\u7814\u7a76\u6210\u679c\u4ee5\u201cVisual in vivo Degradation of Injectable Hydrogel by Real-time and Non-invasive Tracking Using Carbon Nanodots as Fluorescent Indicator\u201d\u4e3a\u9898\u53d1\u8868\u5728\u56fd\u9645\u751f\u7269\u6750\u6599\u9886\u57df\u7684\u6743\u5a01\u671f\u520aBiomaterials\u4e0a\uff08\u5f71\u54cd\u56e0\u5b508.40\uff0cDOI\uff1a10.1016\/j.biomaterials.2017.08.039\uff09\uff0c\u4e3a\u5b9e\u65f6\u65e0\u521b\u53ef\u89c6\u5316\u76d1\u6d4b\u751f\u7269\u6750\u6599\u4f53\u5185\u964d\u89e3\u63d0\u4f9b\u4e86\u65b0\u578b\u8367\u5149\u63a2\u9488\u3002\u8bba\u6587\u7b2c\u4e00\u4f5c\u8005\u4e3a2013\u7ea7\u535a\u58eb\u751f\u738b\u78ca\uff0c\u674e\u4fdd\u5f3a\u526f\u6559\u6388\u4e3a\u552f\u4e00\u901a\u8baf\u4f5c\u8005\uff0c\u54c8\u5de5\u5927\u4e3a\u7b2c\u4e00\u7f72\u540d\u5355\u4f4d\u3002<\/p>\n

\u3010\u56fe\u6587\u5bfc\u8bfb\u3011<\/strong><\/p>\n

\u56fe1. CNDs\u7684\u8868\u5f81\u548c\u4f53\u5916\u7ec6\u80de\u6bd2\u6027 <\/strong><\/p>\n

\"\"<\/p>\n

A. CNDs\u7684TEM\u56fe\u50cf(\u5e73\u5747\u76f4\u5f84= 5.1\u00b11.2 nm)\u548c\u9ad8\u500d\u7387HR-TEM\u56fe\u7247\uff1b\u63d2\u56fe\u4e3a\u5feb\u901f\u5085\u91cc\u53f6\u53d8\u6362\u884d\u5c04\u6a21\u5f0f\uff1b\u4ee5\u53ca\u5728\u7845\u57fa\u7247\u4e0a\u7684CNDs\u7684AFM\u56fe\u50cf\uff1b <\/p>\n

B. CNDs\u7a00\u91ca\u60ac\u6d4a\u6db2\u7684\u7d2b\u5916\u53ef\u89c1\u5438\u6536\u5149\u8c31\u3002\u5de6\u4fa7\u63d2\u56fe\u4e3aCNDs\u60ac\u6d4a\u6db2\u5728\u65e5\u5149\u4e0b\u7684\u7167\u7247\uff0c\u53f3\u4fa7\u63d2\u56fe\u4e3aCNDs\u60ac\u6d4a\u6db2\u5728\u6ce2\u957f\u4e3a365 nm\u7684\u7d2b\u5916\u7ebf\u4e0b\u7684\u7167\u7247\uff1b<\/p>\n

C. CNDs\u7684\u6fc0\u52b1\u4f9d\u8d56\u5149\u81f4\u53d1\u5149\u5149\u8c31\u3002\u5f53\u6fc0\u52b1\u6ce2\u957f\u4ece300nm\u589e\u52a0\u5230460nm \u65f6\uff0cPL\u53d1\u5c04\u6ce2\u957f\u8f6c\u6362\u4e3a\u66f4\u957f\u7684\u6ce2\u957f(430-580nm)\u3002\u5f53\u4f7f\u7528375nm\u7684\u6fc0\u52b1\u6ce2\u957f\u65f6\uff0c\u6700\u957f\u7684\u53d1\u5c04\u6ce2\u957f\u662f450nm\uff1b<\/p>\n

D. \u5728\u4e0d\u540c\u6d53\u5ea6\u7684CNDs\u4e2d\u5b55\u80b224\u300136\u548c48\u5c0f\u65f6\u540e\uff0cNIH\/3T3\u7ec6\u80de\u7684\u7ec6\u80de\u6d3b\u529b\u3002\u7531MTT\u6bd4\u8272\u6cd5\u6d4b\u5b9a\u3002\u5728\u4e0d\u540c\u6d53\u5ea6\u7684CNDs\u4e2d\u7684\u7ec6\u80de\u90fd\u670990%\u4ee5\u4e0a\u7684\u7ec6\u80de\u6d3b\u529b\uff1b<\/p>\n

E. \u7528CNDs\u5904\u7406\u8fc7\u7684NIH \/ 3T3\u7ec6\u80de\u7684\u8367\u5149\u533a\u548c\u4eae\u533a\u7684\u5408\u6210\u56fe\u50cf\uff1b<\/p>\n

\u56fe2. CNDs\u7684\u4f53\u5185\u6bd2\u6027\u8bc4\u4f30<\/strong><\/p>\n

\"\"<\/p>\n

A. \u5404\u79cd\u5668\u5b98(\u5305\u62ec\u809d\u3001\u813e\u3001\u80be\u3001\u5fc3\u3001\u80ba)\u5728\u7b2c1\u5929\u548c\u7b2c14\u5929\u7684\u8367\u5149\u6210\u50cf\u3002\u7528\u751f\u7406\u76d0\u6c34(\u65e0CNDs)\u5904\u7406\u7684\u5c0f\u9f20\u4e3a\u5bf9\u7167\u7ec4\uff1b<\/p>\n

B. \u5404\u79cd\u5668\u5b98(\u5305\u62ec\u809d\u3001\u813e\u3001\u80be\u3001\u5fc3\u3001\u80ba)\u7684\u8367\u5149\u5f3a\u5ea6\u7684\u91cf\u5316\uff1b<\/p>\n

C. \u5728\u7b2c1\u5929\u548c\u7b2c14\u5929\u6ce8\u5c04CNDs\u6eb6\u6db2(\u5242\u91cf23 mg\/ kg)\u7684\u5c0f\u9f20\u7684H&E\u67d3\u8272\u7ec4\u7ec7\u5207\u7247(\u809d\u3001\u813e\u3001\u80be\u3001\u5fc3\u3001\u80ba) \uff1b<\/p>\n

\u56fe3. CNDs\u4f5c\u4e3aCNDs\u6742\u5316\u6c34\u51dd\u80f6\u7684\u53ef\u89c6\u5316\u8367\u5149\u6307\u793a\u5242<\/strong><\/p>\n

\"\"<\/p>\n

A. CNDs\u6742\u5316\u6c34\u51dd\u80f6\u4e0eCNDs\u6d53\u5ea6\u7684\u5b9a\u91cf\u8367\u5149\u5f3a\u5ea6\u5bf9\u6bd4\u56fe\u3002\u63d2\u56fe\u4e3a\u4e0d\u540cCNDs\u6d53\u5ea6\u6c34\u51dd\u80f6\u7684\u8367\u5149\u4f2a\u5f69\u8272\u56fe\u50cf\uff1b<\/p>\n

B. \u540c\u5fc3\u73af\u56fe\u5f62(\u5de6)\u548c\u51f8\u9762\u4f53\u56fe\u5f62(\u53f3) \u7684CNDs\u6742\u5316\u5fae\u51dd\u80f6\u7684\u8367\u5149\u56fe\u50cf\uff0c\u5728\u4e0d\u540c\u6fc0\u52b1\u6ce2\u957f(460 ~ 490nm\u548c540 ~ 560 nm)\u4e0b\u5206\u522b\u663e\u793a\u7eff\u8272\u548c\u7ea2\u8272\u8367\u5149\uff1b<\/p>\n

C. CNDs\u6742\u5316\u6c34\u51dd\u80f6\u7684\u5149\u6f02\u767d\u5b9e\u9a8c\u3002\u6bcf\u96945\u5206\u949f\u62cd\u6444\u7684CNDs\u6742\u5316\u6c34\u51dd\u80f6\u7684\u5149\u6f02\u767d\u548c\u66b4\u9732\u4e8e100W\u6c19\u6fc0\u53d1\u6e90\u65f6\u7684FITC\u6742\u5316\u6c34\u51dd\u80f6\u7684\u8367\u5149\u4f2a\u5f69\u8272\u56fe\u50cf\uff1b <\/p>\n

D. \u9009\u62e9\u4e00\u4e2a\u7279\u5b9a\u7684\u533a\u57df(ROI)\u8fdb\u884c\u8367\u5149\u5f3a\u5ea6\u5b9a\u91cf\u8ba1\u7b97\u3002CNDs\u4f5c\u4e3a\u8367\u5149\u6307\u793a\u5242\uff0c\u6bd4\u8367\u5149\u67d3\u6599(\u5982FITC\u548cRhB)\u66f4\u8010\u5149\uff0c\u800c\u4e14\u66f4\u9002\u5408\u4f53\u5185\u53ef\u89c6\u5316\u7814\u7a76\uff1b<\/p>\n

E. CNDs\u6742\u5316\u6c34\u51dd\u80f6\u7684\u5171\u7126\u663e\u5fae\u955c\u56fe\u50cf\uff1a\u5178\u578b\u7684x – z\u5e73\u9762\u56fe\uff0c\u5178\u578b\u7684x – y\u5e73\u9762\u56fe\u548c\u4e0d\u540c\u6df1\u5ea6\u7684\u8367\u5149\u5f3a\u5ea6\u5256\u9762\u3002\u7528568nm\u7684\u6fc0\u5149\u6fc0\u52b1\u7ea2\u8272\u533a\u57df\uff0c\u7136\u540e\u7528605nm\u7684\u5e26\u901a\u6ee4\u6ce2\u5668\u8fc7\u6ee4\u6fc0\u52b1\u6ce2\u957f\u3002<\/p>\n

\u3010\u7814\u7a76\u5185\u5bb9\u3011<\/strong><\/p>\n

\u57fa\u4e8e\u8367\u5149\u6cd5\u53ef\u89c6\u5316\u76d1\u6d4b\u6c34\u51dd\u80f6\u4f53\u5185\u964d\u89e3\u662f\u5b9e\u65f6\u65e0\u521b\u5b9a\u91cf\u8868\u5f81\u6c34\u51dd\u80f6\u964d\u89e3\u7684\u91cd\u8981\u624b\u6bb5\u3002\u7136\u800c\uff0c\u73b0\u6709\u7684\u8367\u5149\u6307\u793a\u964d\u89e3\u65b9\u6cd5\u5b58\u5728\u5c40\u9650\u6027\uff0c\u5982\u6709\u673a\u8367\u5149\u63a2\u9488\u7684\u5149\u6f02\u767d\u548c\u56e0\u8367\u5149\u57fa\u56e2\u5f15\u5165\u5bfc\u81f4\u5e72\u6270\u9ad8\u5206\u5b50\u7684\u964d\u89e3\u884c\u4e3a\u3002\u4e3a\u4e86\u89e3\u51b3\u4ee5\u4e0a\u95ee\u9898\uff0c\u674e\u4fdd\u5f3a\u526f\u6559\u6388\u7b49\u4eba\u5408\u6210\u5e76\u5229\u7528\u4f4e\u5149\u6f02\u767d\u3001\u53d1\u5c04\u7ea2\u5149\u548c\u826f\u597d\u751f\u7269\u76f8\u5bb9\u6027\u7684\u7eb3\u7c73\u78b3\u70b9\u4e3a\u8367\u5149\u6307\u793a\u5242\uff0c\u5b9e\u73b0\u4e86\u5b9e\u65f6\u65e0\u521b\u76d1\u6d4b\u53ef\u6ce8\u5c04\u6c34\u51dd\u80f6\u7684\u4f53\u5185\u4e0e\u4f53\u5916\u964d\u89e3\uff0c\u7cfb\u7edf\u8bc4\u4ef7\u4e86\u7eb3\u7c73\u78b3\u70b9\u7684\u751f\u7269\u76f8\u5bb9\u6027\uff0c\u5efa\u7acb\u4e86\u5b9e\u65f6\u65e0\u521b\u53ef\u89c6\u5316\u5b9a\u91cf\u8bc4\u4ef7\u6c34\u51dd\u80f6\u964d\u89e3\u7684\u6570\u5b66\u6a21\u578b\u3002\u4f53\u5916\u4e0e\u4f53\u5185\u6bd2\u6027\u6d4b\u8bd5\u8868\u660e\u7eb3\u7c73\u78b3\u70b9\u5bf9\u7ec6\u80de\u548c\u5468\u56f4\u7ec4\u7ec7\u65e0\u6bd2\uff0c\u5e76\u5177\u6709\u826f\u597d\u751f\u7269\u76f8\u5bb9\u6027\u3002\u5f53\u51dd\u80f6\u4e0d\u964d\u89e3\u65f6\uff0c\u5305\u57cb\u5728\u6c34\u51dd\u80f6\u7684\u7eb3\u7c73\u78b3\u70b9\u4e0d\u4f1a\u91ca\u653e\uff1b\u5f53\u51dd\u80f6\u964d\u89e3\u65f6\uff0c\u57fa\u4e8e\u91cd\u91cf\u6cd5\u548c\u57fa\u4e8e\u7eb3\u7c73\u78b3\u70b9\u7684\u8367\u5149\u53ef\u89c6\u5316\u6cd5\u5448\u73b0\u76f8\u4f3c\u964d\u89e3\u52a8\u529b\u5b66\u66f2\u7ebf\uff0c\u5e76\u5efa\u7acb\u4e86\u5b9a\u91cf\u8868\u5f81\u6c34\u51dd\u80f6\u4f53\u5916\u964d\u89e3\u7684\u6570\u5b66\u6a21\u578b\u4ee5\u4fbf\u4e8e\u9884\u6d4b\u6c34\u51dd\u80f6\u7684\u4f53\u5185\u964d\u89e3\u884c\u4e3a\uff0c\u6700\u7ec8\u5b9e\u73b0\u4e86\u5b9e\u65f6\u65e0\u521b\u53ef\u89c6\u5316\u5b9a\u91cf\u8868\u5f81\u751f\u7269\u6750\u6599\u4f53\u5185\u964d\u89e3\u3002\u57fa\u4e8e\u7eb3\u7c73\u78b3\u70b9\u5b9e\u65f6\u65e0\u521b\u53ef\u89c6\u5316\u76d1\u6d4b\u751f\u7269\u6750\u6599\u964d\u89e3\u65b9\u6cd5\u4e3a\u7ec4\u7ec7\u5de5\u7a0b\u4e2d\u7684\u751f\u7269\u6750\u6599\u53ef\u63a7\u964d\u89e3\u548c\u4fbf\u6377\u7b5b\u9009\u4f53\u5185\u964d\u89e3\u63d0\u4f9b\u4e86\u65b0\u7684\u7814\u7a76\u65b9\u6cd5\u3002<\/p>\n

\u539f\u6587\u94fe\u63a5\uff1ahttp:\/\/news.hit.edu.cn\/cd\/b9\/c416a183737\/page.htm<\/a><\/p>\n

\u6587\u732e\u94fe\u63a5\uff1aVisual in vivo Degradation of Injectable Hydrogel by Real-time and Non-invasive Tracking Using Carbon Nanodots as Fluorescent Indicator<\/a>\uff08Biomaterials\uff0c2017\uff0cDOI\uff1a10.1016\/j.biomaterials.2017.08.039\uff09<\/p>\n

\u672c\u6587\u7531\u6750\u6599\u4eba\u7f16\u8f91\u90e8\u5b5f\u4ee4\u6653\u7f16\u8f91\uff0c\u70b9\u6211\u52a0\u5165\u6750\u6599\u4eba\u7f16\u8f91\u90e8<\/a>\u3002<\/p>\n

\u6750\u6599\u6d4b\u8bd5\uff0c\u6570\u636e\u5206\u6790\uff0c\u4e0a\u6d4b\u8bd5\u8c37<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\u3010\u6210\u679c\u7b80\u4ecb\u3011 \u54c8\u5de5\u5927\u6750\u6599\u5b66\u9662\u5468\u7389\u9662\u58eb\u56e2\u961f\u9aa8\u5e72\u6210\u5458\u674e\u4fdd\u5f3a\u526f\u6559\u6388\u5728\u57fa\u4e8e\u7eb3\u7c73\u78b3\u70b9\uff08CNDs\uff09\u5b9e\u65f6\u65e0\u521b\u53ef\u89c6\u5316\u76d1\u6d4b\u751f\u7269\u6750\u6599\u4f53\u5185\u964d\u89e3\u65b9\u9762\u53d6\u5f97\u91cd\u8981\u8fdb\u5c55\uff0c\u7814\u7a76\u6210\u679c\u4ee5\u201cVisua…<\/p>\n","protected":false},"author":608,"featured_media":100940,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2],"tags":[75,33],"class_list":["post-100938","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-keji","tag-biomaterial","tag-nano"],"_links":{"self":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/100938"}],"collection":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/users\/608"}],"replies":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=100938"}],"version-history":[{"count":7,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/100938\/revisions"}],"predecessor-version":[{"id":101271,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/posts\/100938\/revisions\/101271"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=\/wp\/v2\/media\/100940"}],"wp:attachment":[{"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=100938"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=100938"},{"taxonomy":"post_tag","embeddable":true,"href":"\/\/m.iemloyee.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=100938"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}